CN103004533A - Method for preventing cyclamen wilt disease - Google Patents
Method for preventing cyclamen wilt disease Download PDFInfo
- Publication number
- CN103004533A CN103004533A CN2012104916041A CN201210491604A CN103004533A CN 103004533 A CN103004533 A CN 103004533A CN 2012104916041 A CN2012104916041 A CN 2012104916041A CN 201210491604 A CN201210491604 A CN 201210491604A CN 103004533 A CN103004533 A CN 103004533A
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- CN
- China
- Prior art keywords
- cyclamen
- bacterium
- bacteria
- preventing
- ivyleaf cyclamen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 11
- 241000612152 Cyclamen hederifolium Species 0.000 title claims description 27
- 201000010099 disease Diseases 0.000 title abstract description 12
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title abstract description 12
- 229930186364 cyclamen Natural products 0.000 title abstract 4
- 241000894006 Bacteria Species 0.000 claims abstract description 30
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 239000002689 soil Substances 0.000 claims abstract description 5
- 230000000694 effects Effects 0.000 claims abstract description 4
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims description 14
- 241000223218 Fusarium Species 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 230000005764 inhibitory process Effects 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 abstract description 3
- 230000001580 bacterial effect Effects 0.000 abstract description 2
- 239000013043 chemical agent Substances 0.000 abstract description 2
- 241000612153 Cyclamen Species 0.000 abstract 3
- 241000223221 Fusarium oxysporum Species 0.000 abstract 3
- 239000012880 LB liquid culture medium Substances 0.000 abstract 1
- 230000003385 bacteriostatic effect Effects 0.000 abstract 1
- 230000000443 biocontrol Effects 0.000 abstract 1
- 230000007547 defect Effects 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 9
- 241000196324 Embryophyta Species 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000000575 pesticide Substances 0.000 description 4
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000012137 tryptone Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 239000006391 Luria-Bertani Medium Substances 0.000 description 1
- 241000206591 Peptococcus Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000009363 floriculture Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 230000028744 lysogeny Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for preventing a cyclamen wilt disease. The cyclamen is irrigated by 100-fold fermentation liquid of bacteria A with confrontation effect with fusarium oxysporum schlecht every two weeks. The bacterial liquid A is prepared by the following steps: (1) screening out one part of bacteria from soil and performing confrontation cultivation on the bacteria and the fusarium oxysporum schlecht respectively so as to obtain an obvious bacteriostatic ring of the bacteria A on the fusarium oxysporum schlecht; and (2) shaking the bacteria A on an LB liquid culture medium in advance, inoculating into a PS culture medium in a ratio of 1:1,000, and cultivating in a shaker at the temperature of 37 DEG C and at the rotating speed of 170 rpm/min for 48 h. The invention provides a biocontrol technology for cyclamen so as to overcome the defect of high pollution of chemical agents.
Description
Technical field
The invention belongs to field of floriculture methods, particularly in a kind of ivyleaf cyclamen cultivation process, prevent the method for fusarium wilt.
Background technology
On producing; ivyleaf cyclamen often can be subject to the serious threat of fusarium wilt; the fusarium wilt of ivyleaf cyclamen is a kind of soil-borne disease; the symptom initial stage from plant apart from ground near blade to begin flavescence withered, later on gradually to vertical spread, the blackening of root bead is rotted; sooner or later normal; wilt noon, and like the lack of water shape, its cause of disease is sharp Fusariumsp.
The fusarium wilt of ivyleaf cyclamen can only prevent its expansion in case morbidity is difficult to cure, and therefore, anti-ratio is controlled more important.For a long time, chemical pesticide is the Main Means of this disease of control always, but along with a large amount of uses of chemical pesticide, the series of problems such as environmental pollution, pesticide resistance and residue of pesticide are troubling.Seek new diseases prevention means, effectively control the harm of ivyleaf cyclamen fusarium wilt, become problem in the urgent need to address in the production practices.
Summary of the invention
The purpose of this invention is to provide a kind of method of preventing the ivyleaf cyclamen fusarium wilt, effectively prevent the generation of ivyleaf cyclamen fusarium wilt.
Realize that above-mentioned purpose adopts following technical proposals: a kind of method of preventing the ivyleaf cyclamen fusarium wilt, using in per two weeks of ivyleaf cyclamen with sharp Fusariumsp has 100 times of zymotic fluids of the A bacterium of face-off effect to water.
Further, described A bacterium liquid obtains by following steps:
(1) from soil, screen a part of bacterium, with these bacteriums respectively with the cultivation that stands facing each other of sharp Fusariumsp, thereby obtain a kind of bacterium A sharp Fusariumsp is produced obvious inhibition zone;
(2) A bacterium liquid is shaken on the LB liquid nutrient medium in advance, then with the ratio access PS medium of 1:1000, the rotating speed with 170rpm/min in 37 ℃ of shaking tables is cultivated 48h.
The LB medium generally is interpreted as the Luria-Bertani medium, and its invention people is Bel's tower Buddhist nun (Giuseppe Bertani), and this name derives from the lysogeny broth of English, instant bacterial context soup.
The prescription of LB medium is as follows:
Tryptone (Tryptone) 10g/L; Yeast extract (Yeast extract) 5g/L; Sodium chloride (NaCl) 10g/L.
The PS medium is used for the cultivation of peptococcus, and composition consists of:
The present invention is intended to remedy the heavy-polluted defective of chemical agent, and the biological and ecological methods to prevent plant disease, pests, and erosion technology of a kind of ivyleaf cyclamen is provided.With the present invention the fusarium wilt of ivyleaf cyclamen is prevented, long run test is 3 years in the greenhouse of plantation ivyleaf cyclamen, and effect is very obvious, and the fusarium wilt of ivyleaf cyclamen can be controlled well, and the plant strain growth everyway is very healthy, can supply according to schedule launch.
Embodiment
Embodiment 1
At first, from soil, screen a part of bacterium, with these bacteriums respectively with the cultivation that stands facing each other of sharp Fusariumsp, thereby obtain a kind of bacterium A sharp Fusariumsp is produced obvious inhibition zone.Then, A bacterium liquid is shaken on the LB liquid nutrient medium in advance, then with the ratio access PS medium of 1:1000, the rotating speed with 170rpm/min in 37 ℃ of shaking tables is cultivated 48h.
Get 200 ivyleaf cyclamen seedlings that upgrowth situation is similar, carry out maintenance with the matrix of carrying wilt, wherein 100 ivyleaf cyclamen per two all 100 times of zymotic fluids with the A bacterium water, all the other 100 are watered with clear water, other environmental conditions, identical such as illumination, humidity, temperature, liquid manure etc., observe statistics incidence of disease discovery after 6 months, be 16% with the ivyleaf cyclamen incidence of disease after the A ferment product pouring, and be 87% with the incidence of disease of the ivyleaf cyclamen of clear water pouring.Thereby reach a conclusion, this bacterium has certain preventive and therapeutic effect to the fusarium wilt of ivyleaf cyclamen.
Embodiment 2
At first, from soil, screen a part of bacterium, with these bacteriums respectively with the cultivation that stands facing each other of sharp Fusariumsp, thereby obtain a kind of bacterium A sharp Fusariumsp is produced obvious inhibition zone.Then, A bacterium liquid is shaken on the LB liquid nutrient medium in advance, then with the ratio access PS medium of 1:1000, the rotating speed with 170rpm/min in 37 ℃ of shaking tables is cultivated 48h.
In same greenhouse, plant 2000 same batch ivyleaf cyclamen seedling, wherein 1000 per two all A bacterium liquid with 100 times water once per month, and other 1000 be left intact, found that, 1000 ivyleaf cyclamen incidences of disease that water with 100 times of bacterium liquid are 0.24%, and the incidence of disease of 1000 ivyleaf cyclamens that are left intact is 7.6%, and, with the ivyleaf cyclamen plant of 100 times of bacterium liquid pourings, blade is intact without unusual, and the flower of leaving is also vivid without unusual.
Claims (2)
1. method of preventing the ivyleaf cyclamen fusarium wilt, using in per two weeks of ivyleaf cyclamen with sharp Fusariumsp has 100 times of zymotic fluids of the A bacterium of face-off effect to water.
2. a kind of method of preventing the ivyleaf cyclamen fusarium wilt according to claim 1 is characterized in that, described A bacterium liquid obtains by following steps:
(1) from soil, screen a part of bacterium, with these bacteriums respectively with the cultivation that stands facing each other of sharp Fusariumsp, thereby obtain a kind of bacterium A sharp Fusariumsp is produced obvious inhibition zone;
(2) A bacterium liquid is shaken on the LB liquid nutrient medium in advance, then with the ratio access PS medium of 1:1000, the rotating speed with 170rpm/min in 37 ℃ of shaking tables is cultivated 48h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104916041A CN103004533A (en) | 2012-11-27 | 2012-11-27 | Method for preventing cyclamen wilt disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104916041A CN103004533A (en) | 2012-11-27 | 2012-11-27 | Method for preventing cyclamen wilt disease |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103004533A true CN103004533A (en) | 2013-04-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012104916041A Pending CN103004533A (en) | 2012-11-27 | 2012-11-27 | Method for preventing cyclamen wilt disease |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103004533A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114107071A (en) * | 2021-12-14 | 2022-03-01 | 山西省农业科学院园艺研究所 | A kind of separation and purification method of cyclamen fusarium wilt pathogen |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20050072341A (en) * | 2004-01-06 | 2005-07-11 | 학교법인고려중앙학원 | Streptomyces roseoflavus ls-a24 strain effective against plant pathogenic fungi and fungicides produced from it |
| CN101250495A (en) * | 2008-03-03 | 2008-08-27 | 中国热带农业科学院环境与植物保护研究所 | Plant pathogenic fungus antagonistic bacterium and application thereof in preventing and treating plant diseases |
| CN103146586A (en) * | 2013-03-11 | 2013-06-12 | 赵斌 | Trichoderma harzianum strain for controlling plant fungus diseases and application thereof |
| US8652813B2 (en) * | 2009-11-25 | 2014-02-18 | Codexis, Inc. | Recombinant Thermoascus aurantiacus β-glucosidase variants |
-
2012
- 2012-11-27 CN CN2012104916041A patent/CN103004533A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20050072341A (en) * | 2004-01-06 | 2005-07-11 | 학교법인고려중앙학원 | Streptomyces roseoflavus ls-a24 strain effective against plant pathogenic fungi and fungicides produced from it |
| CN101250495A (en) * | 2008-03-03 | 2008-08-27 | 中国热带农业科学院环境与植物保护研究所 | Plant pathogenic fungus antagonistic bacterium and application thereof in preventing and treating plant diseases |
| US8652813B2 (en) * | 2009-11-25 | 2014-02-18 | Codexis, Inc. | Recombinant Thermoascus aurantiacus β-glucosidase variants |
| CN103146586A (en) * | 2013-03-11 | 2013-06-12 | 赵斌 | Trichoderma harzianum strain for controlling plant fungus diseases and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 周秀华等: "三种拮抗菌对苗木立枯病病原菌的影响", 《北方园艺》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114107071A (en) * | 2021-12-14 | 2022-03-01 | 山西省农业科学院园艺研究所 | A kind of separation and purification method of cyclamen fusarium wilt pathogen |
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Application publication date: 20130403 |