CN102993147B - 4-chromone and 4-chromanone compound and purposes - Google Patents

Abstract

The invention belongs to the technical field of medicine, and relates to the medical application of chromanone compounds and their analogues. The chromanone compounds and their analogs and pharmaceutically applicable acid addition salts are used as platelet aggregation inhibitors, and their general structural formula is as follows. The application of the new compound of the present invention as an effective anti-platelet drug, especially in the preparation of drugs for the prevention or treatment of coronary syndrome, myocardial infarction, myocardial ischemia, cardiovascular and cerebrovascular diseases caused by platelet aggregation, and treatment of thrombus Formation, ischemia, stroke, restenosis or inflammation, ie administering an effective amount of a compound of the invention.

Description

4-chromone and 4-chromanone compound and application

technical field

The invention belongs to the technical field of medicine, and relates to the medical application of chromanone compounds and their analogues, which are novel anti-platelet aggregation compounds.

Background technique

Thrombosis can lead to acute myocardial infarction, stroke, pulmonary embolism and other heart, brain, and pulmonary circulation diseases. It is also a common complication in surgery and a factor of reocclusion after interventional angioplasty. Since platelet aggregation plays an important role in thrombus formation, antiplatelet drugs have become the main drugs for the prevention and treatment of these diseases. Antiplatelet drugs, also known as platelet function inhibitors, can inhibit the adhesion, aggregation and release of platelets, inhibit thrombus formation, and prolong the shortened platelet survival in patients with thrombotic diseases. As an antiplatelet drug, it should be required to have no significant effect on the body's bleeding and coagulation process at a clinically applied dose, that is, not cause bleeding and other adverse reactions.

At present, it is found that chromone, chromanone and chroman compounds have certain activity in anti-platelet aggregation, such as 2-amino-4-chromone, 3-dimethylaminomethyl-4-chromone, 3-aminomethyl Base-4-chromanone, etc. On this basis, we have obtained the compound of the present invention, which has strong anti-platelet aggregation activity.

Contents of the invention

The purpose of the present invention is to provide a class of novel substituted ether chromones, chromanones and chroman derivatives and their preparation methods.

The present invention includes a composition for inhibiting the loss of platelets in mammals, inhibiting the formation of platelet aggregates, inhibiting the formation of fibrin, inhibiting thrombus formation, and inhibiting the formation of emboli, the composition comprising the compound of the present invention and the pharmaceutically acceptable carrier. These compositions may optionally include anticoagulants, antiplatelet agents and thrombolytic agents. The composition can be added to blood, blood products or mammalian organs to achieve the desired inhibitory effect.

The invention also provides methods of inhibiting or treating abnormal proteolysis, and methods for the treatment of: myocardial infarction; unstable angina; stroke; restenosis; deep vein thrombosis; disseminated intravascular coagulation; hemodialysis; cardiopulmonary bypass; adult respiratory distress; endotoxic shock; rheumatoid arthritis; ulcerative colitis; induration; metastasis; hypercoagulability during chemotherapy; asen Haimer's disease; Dawn's syndrome; intraocular fibrin formation and wound healing. Other uses of the compounds of the invention are as anticoagulants, embedded in or physically attached to materials used in the manufacture of devices for blood collection, blood circulation and blood storage, such as catheters, hemodialyzers, blood collection syringes With test tube, blood line and stent.

The invention also includes a method for reducing thrombus formation on a surface in a mammal by covalently or non-covalently binding a compound of the invention to the surface.

In another aspect, the invention includes compositions useful for imaging thrombus in vivo in mammals, the compositions comprising a compound of the invention capable of being detected in vitro. Preferred compositions comprise a compound of the invention and a detectable label, such as a radioactive or paramagnetic atom.

In another aspect, the present invention provides a diagnostic composition useful for imaging thrombi in mammals, the composition comprising a pharmaceutically acceptable carrier and a diagnostically effective amount of a compound or composition of the present invention.

In another aspect, the invention includes methods useful for imaging thrombi in mammals.

The present invention includes stereoisomers as well as optical isomers, such as mixtures of enantiomers as well as individual enantiomers and diastereomers, which arise due to structural asymmetries in the selected series of compounds. The compounds of the present invention may also have polymorphic forms, all polymorphic forms are also included in the present invention.

The compounds of the invention may also be in the form of solvates, especially hydrates. Hydration can occur during manufacture of the compound or a composition comprising the compound, or over time due to the hygroscopic nature of the compound.

Certain of the compounds of the present invention are derivatives known as prodrugs.

Pharmaceutically acceptable salts of the compounds of the present invention (in the form of water-soluble or oil-soluble products or dispersible products) include conventional non-toxic salts or quaternary ammonium salts, which are formed, for example, from inorganic or organic acids or bases. Examples of acid addition salts include acetate, adipate, alginate, aspartate, benzoate, benzenesulfonate, bisulfate, butyrate, citrate, camphoric acid Salt, camphorsulfonate, cypionate, gluconate, lauryl sulfate, ethanesulfonate, fumarate, glycerophosphate, hemisulfate, heptanoate, hexanoate, Hydrochloride, hydrobromide, hydroiodide, 2-hydroxyethanesulfonate, lactate, maleate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, Oxalate, pamoate, pectinate, persulfate, 3-phenylpropionate, picrate, pivalate, propionate, succinate, sulfate, tartrate, sulfur Cyanate, Tosylate and Undecanoate. Alkali salts include ammonium salts, alkali metal salts such as sodium and potassium, alkaline earth metal salts such as calcium and magnesium, salts of organic bases such as dicyclohexylamine, N-methyl-D-glucamine Salts, and salts of amino acids, such as arginine, lysine, etc., and basic nitrogen-containing groups can be quaternized with such reagents, such as lower alkyl halides, such as methyl, ethyl, propyl and butyl chloride, bromine and iodide; dialkyl sulfates such as dimethyl sulfate, diethyl sulfate, dibutyl sulfate and dipentyl sulfate; long-chain halides such as decyl, lauryl, Myristyl and stearyl chlorine, bromine and iodide; aralkyl halides, such as benzyl and phenethyl bromide, etc. Preferred acids for the formation of acid addition salts include hydrochloric acid and acetic acid.

For their use, the potency of the enzyme inhibitory properties and other biochemical parameters of the compounds of the invention are determined according to standard biochemical procedures well known in the art. The actual dosage range employed will depend on the nature and severity of the disease state in the patient or animal being treated, as will be determined by the attending diagnostician. It is expected that a useful dosage range will be about 0.01-10 mg/kg per day to achieve an effective therapeutic effect.

The compounds of the invention can be used for a variety of therapeutic purposes. The compounds of the present invention are useful in the treatment or prevention of conditions characterized by abnormal venous or arterial thrombosis involving the generation or action of thrombin, including but not limited to deep vein thrombosis; occurring in septic shock, viral infections and cancer processes disseminated intravascular coagulation; myocardial infarction; stroke; coronary artery bypass; intraocular fibrin formation; hip reduction; thrombosis resulting from thrombolytic therapy or percutaneous transluminal coronary angioplasty. Other uses include use as an anticoagulant, embedded in or physically attached to materials used in the manufacture of devices for blood collection, blood circulation and blood storage, such as catheters, hemodialysis machines, blood collection syringes and test tubes and blood lines. The compounds of the invention may also be used as anticoagulants in extracorporeal blood circulation.

Metal stents were shown to reduce restenosis, but were also thrombogenic. One strategy to reduce stent thrombosis is to coat, embed, adsorb or covalently covalently thrombin inhibitors on the stent surface. The compounds of the invention are useful for this purpose. Compounds of the invention may be incorporated or embedded in soluble and/or biodegradable polymers, which are then applied to the stent material. Such polymers may include polyvinylpyrrolidone, polyhydroxy-propylmethacrylamide-phenol, polyhydroxyethyl-aspartamide-phenol, polyethylene oxide-polylysine substituted with palmitoyl residues, Polylactic acid, polyglycolic acid, copolymers of polylactic acid and polyglycolic acid, polyεcaprolactone, polyhydroxybutyrate, polyorthoester, polyacetal, polydihydropyran, polycyanoacrylate, and water Gels of cross-linked or amphiphilic block copolymers.

Additional utility for the compounds of the present invention has been found by virtue of platelet action on host cell types such as smooth muscle cells, endothelial cells and neutrophils for adult respiratory distress syndrome; inflammatory response; wound healing; reperfusion injury; atherosclerosis and treatment or prevention of restenosis secondary to injury such as balloon angioplasty, atherectomy, and arterial stent placement.

When used as a platelet aggregation inhibitor, the effective dose of the compound of the present invention can be administered in the dosage range of about 0.1-500 mg/kg body weight, preferably in the range of 0.1-10 mg/kg body weight, and the dosage regimen is once a day or 2 -4 times.

When used as platelet aggregation inhibitors, the compounds of the invention may be used in combination with thrombolytic agents such as tissue plasminogen activator, streptokinase and urokinase. Additionally, the compounds of the present invention may be used in combination with other antithrombotic or anticoagulant agents, such as, but not limited to, fibrinogen antagonists and thromboxane receptor antagonists.

Platelet aggregation inhibitors can be coupled to soluble polymers which serve as targetable drug carriers. Such polymers may include polyvinylpyrrolidone, pyran copolymer, polyhydroxy-propylmethacrylamide-phenol, polyhydroxyethyl-aspartamide-phenol, or polyethylene oxide substituted with palmitoyl residues. - Polylysine. Moreover, thrombin inhibitors can be coupled with a class of biodegradable polymers that can be used to achieve controlled drug release, such as polylactic acid, polyglycolic acid, copolymers of polylactic acid and polyglycolic acid, polyεcaprolact Crosslinked or amphiphilic block copolymers of esters, polyhydroxybutyrate, polyorthoesters, polyacetals, polydihydropyrans, polycyanoacrylates, and hydrogels.

The pharmaceutical composition of the present invention can be administered by any means. For example, administration can be by parenteral, subcutaneous, intravenous, intramuscular, intraperitoneal, transdermal, buccal or ocular routes. Alternatively, concurrent administration may be by oral route. The dosage administered will depend on the age, health and weight of the subject, the type of concurrent therapy (if any), frequency of treatment, and the nature of the desired effect.

Besides the pharmaceutically active compound, the pharmaceutical preparations may also contain suitable pharmaceutically acceptable carriers comprising excipients and auxiliaries which facilitate processing of the active compound into preparations which can be used pharmaceutically.

The pharmaceutical formulations of the present invention are prepared by conventional mixing, granulating, pelletizing, dissolving, or freeze-drying processes. Thus, pharmaceutical preparations for oral administration can be obtained by mixing the active compound with a solid excipient, adding suitable auxiliaries, then grinding the resulting mixture, and processing the mixture of granules into tablets or dragee cores.

Suitable excipients are especially fillers, for example sugars, such as lactose or sucrose, mannitol or sorbitol, cellulose preparations and/or calcium phosphates, such as tricalcium phosphate or calcium hydrogen phosphate, and binders, For example starch pastes such as corn starch, wheat starch, rice starch, potato starch, gelatin, tragacanth, methylcellulose, hydroxypropylmethylcellulose, sodium carboxymethylcellulose, and/or polyvinylpyrrolidone. It is also possible to add disintegrants, such as the aforementioned starch and carboxymethyl starch, cross-linked polyvinylpyrrolidone, agar, alginic acid or its salts, such as sodium alginate. Auxiliaries are especially glidants and lubricants, for example silica, talc, stearic acid or its salts, such as magnesium stearate or calcium stearate, and/or polyethylene glycol. Optionally containing gum arabic, talc, polyvinylpyrrolidone, polyethylene glycol and/or titanium dioxide, lacquer solutions and suitable organic solvents or solvent mixtures. To form coatings resistant to gastric acid, solutions of suitable cellulose preparations, such as cellulose acetate phthalate or hydroxypropylmethylcellulose phthalate, are used. Dyestuffs or pigments may be added to the tablets or dragee coatings, eg, for identification or to delineate combinations of active compound(s).

Other pharmaceutical preparations that can be used orally include push-fit capsules made of gelatin, and soft, sealed capsules made of gelatin and a plasticizer such as glycerin or sorbitol. The push-fit capsules can contain the active compounds in granule form which may be admixed with filler such as lactose, binders such as starches, and/or lubricants such as talc or magnesium stearate and, optionally, stabilizers. In soft capsules, the active compounds are preferably dissolved or suspended in suitable liquids, such as fatty oils, or liquid paraffin. In addition, stabilizers may also be added.

Formulations suitable for parenteral administration include aqueous solutions of water-soluble active compounds, such as water-soluble salts, alkaline solutions and cyclodextrin inclusion complexes. Especially preferred base salts are ammonium salts prepared, for example, from Tris, choline hydroxide, Bis-Tris propane, N-methylglucamine or arginine. One or more modified or unmodified cyclodextrins may be used to stabilize and increase the water solubility of the compounds of the invention.

Alternatively, the active compounds may be administered as appropriate oily injection suspensions. Suitable lipophilic solvents or vehicles include fatty oils, such as sesame oil, or synthetic fatty acid esters, such as ethyl oleate or triglycerides or polyethylene glycol-400 (the compound is soluble in PEG-400). Aqueous injection suspensions may contain substances which increase the viscosity of the suspension, such as sodium carboxymethyl cellulose, sorbitol and/or dextran. Optionally, the suspension may also contain stabilizers.

Compounds of the invention can be labeled with radioactive iodine using an exchange reaction. Hot iodine was exchanged for cold iodine as is well known in the art.

The invention also includes compositions useful for imaging thrombi in vivo, wherein the composition consists of a compound of the invention complexed to a radioactive atom, or consists of a compound of the invention complexed to a paramagnetic atom. obtained using complexation techniques well known in the art.

The invention also includes diagnostic compositions useful for imaging thrombi in vivo, comprising a pharmaceutically acceptable carrier and a diagnostically effective amount of a compound of the invention.

The "diagnostically effective amount" of the composition required per dose will depend on the route of administration, the type of treatment being treated and the particular physical characteristic in question. These factors and their relationship to determining dosage are well known to those skilled in the art of medical diagnostics. Furthermore, the diagnostically effective amount and method of administration can be adjusted to achieve an optimal therapeutic effect, but will also depend on factors such as body weight, diet, concomitant drug therapy and other factors that will be considered by those skilled in the medical arts. In any aspect, the dose used for imaging should be sufficient to detect the presence of the imaging agent in the targeted thrombus site. Typically, radiographic imaging requires that the pharmaceutical compositions of the present invention provide doses of about 5-20 μCi, preferably about 10 μCi. Magnetic resonance imaging requires a dose of about 0.001-5 mmol/kg, preferably about 0.005-0.5 mmol/kg, of the compound of the invention complexed with a paramagnetic atom be provided. In both cases, it is known in the art that the actual dosage will depend on the location of the thrombus.

"Pharmaceutically acceptable carriers" for use in vivo are well known in the field of pharmacy. The pharmaceutical composition of the present invention can be formulated with a pharmaceutically acceptable carrier as sterile solution or suspension for injection, solid form suitable for solution or suspension in liquid prior to injection, or emulsion. Suitable excipients are, for example, water, saline, dextrose, mannitol, lactose, lecithin, albumin, sodium glutamate, cysteine hydrochloride and the like. In addition, injectable pharmaceutical compositions may contain small amounts of non-toxic auxiliary substances such as wetting agents, pH buffering agents and the like. Absorption enhancement (eg liposomes) may be employed if desired.

The invention also encompasses diagnostic compositions prepared for storage or administration which additionally contain preservatives, stabilizers and dyes. For example, sodium benzoate, esters of sorbic acid and p-hydroxybenzoic acid may be added as preservatives. In addition, antioxidants and suspending agents may be used.

The in vivo imaging method of the present invention also offers several advantages over previous imaging techniques for detecting or monitoring the presence, size, regression or growth of thrombus. In particular, the compounds, compositions and diagnostic compositions provided herein have been designed to bind very tightly to thrombin, which is associated with thrombus, thereby reducing the generation of circulating radioactivity or parasitic activity by unbound imaging agents. Magnetic "background". Furthermore, in vivo imaging by intracoronary injection of compounds, compositions or diagnostic compositions of the invention is expected to be nearly instantaneous, since these imaging agents immediately saturate thrombin bound to the thrombus.

Accordingly, the invention also includes a method for imaging thrombi in vivo comprising the steps of: 1. administering a diagnostically acceptable amount of a compound, composition or diagnostic composition of the invention, and 2. detecting intravascular of thrombus. Methods used to monitor thrombus size, location and number, and thrombus dissolution or growth. Where the method uses the compound, composition or diagnostic composition in vivo, "administering" is effected parenterally, either in a systemic or locally directed manner. Systemic administration is achieved by injecting a compound, composition or diagnostic composition of the invention into a suitable and available vein or artery. Locally directed administration is accomplished by injecting a compound, composition or diagnostic composition of the invention at a rate close to blood flow into a vein or artery suspected of containing a thrombus remote from the injection site. This includes, but is not limited to, injection directly into the coronary vasculature to image a coronary thrombus, injection directly into the carotid artery to image a thrombus in the cerebral vasculature, or injection directly into a foot vein to image deep leg Imaging of venous thrombosis.

The mode of release of the composition of the invention to the site of the thrombus is also contemplated by the term "administration". For example, a compound to which a chelating unit is attached can be injected into the body, followed by infusion of a radioactive atom, thereby forming a composition comprising the compound complexed to the radioactive atom in vivo at the thrombus site. Alternatively, a composition comprising a compound complexed with a radioactive atom can be injected into the body.

As previously stated, a "diagnostically effective amount" of a compound, composition or diagnostic composition used in the methods of the invention will depend on the route of administration, the type being treated and the particular medical characteristic being treated. These factors and their relationship to determining dosage are well known to those skilled in the art of medical diagnostics. In any aspect, the dose used for in vivo imaging should be sufficient to detect the presence of the imaging dose in the targeted thrombus site. Typically, radiographic imaging requires that the pharmaceutical composition of the present invention be provided at a dose of about 5-20 μCi, preferably about 10 μCi. Magnetic resonance imaging requires a dose of about 0.001-5 mmol/kg, preferably about 0.005-0.5 mmol/kg, of the compound of the invention complexed with a paramagnetic atom be provided. In both cases, it is known in the art that the actual dosage will depend on the location of the thrombus.

The application of the new compound of the present invention as an effective anti-platelet drug, especially in the preparation of drugs for the prevention or treatment of coronary syndrome, myocardial infarction, myocardial ischemia, cardiovascular and cerebrovascular diseases caused by platelet aggregation, and treatment of thrombus Formation, ischemia, stroke, restenosis or inflammation, ie administering an effective amount of a compound of the invention.

Detailed ways

The following examples illustrate, but do not limit, the methods and compositions of the invention. Other appropriate modifications and adjustments of different conditions and parameters are also normal and obvious to those skilled in the art, and are also within the scope of the present invention.

The synthetic steps for preparing the compounds of the invention are outlined below.

Example 1: 7-[2-(1-pyrrolidinyl)ethoxy]-4-chromone

Take 10mmol 7-hydroxy-4-chromone, add 10mmol 1-chloroethylpyrrolidine hydrochloride, 10mmol K ₂ CO ₃ , and 1mmol KI, add an appropriate amount of acetone, reflux for 12 hours, filter with suction, and discard Inorganic salt, after concentrating the solution, recrystallize the obtained solid to obtain white crystal 7-[2-(1-pyrrolidinyl)ethoxy]-4-chromone with a yield of 51%. MS m/z (M): 259.12; ¹ HNMR (CDCl ₃ ): δ1.59 (4H, m), 2.25 (4H, q), 2.78 (2H, m), 4.04 (2H, m), 6.35 (1H , d, J=9Hz), 6.43(1H, d, J=3Hz), 6.52(1H, dd, J1=9Hz d, J2=3Hz), 7.20(1H, d, J=9Hz), 7.53(1H, d, J=9Hz).

Example 2: 7-[2-(1-piperidinyl)ethoxy]-4-chromone

According to the method of Example 1, white crystal 7-[2-(1-piperidinyl)ethoxy]-4-chromone was obtained with a yield of 56%. MS m/z (M): 273.33; ¹ HNMR (CDCl ₃ ): δ1.50 (6H, m), 2.24 (4H, m), 2.78 (2H, m), 4.04 (2H, m), 6.35 (1H , d, J=9Hz), 6.43(1H, d, J=3Hz), 6.52(1H, dd, J1=9Hz d, J2=3Hz), 7.20(1H, d, J=9Hz), 7.53(1H, d, J=9Hz).

Example 3: 7-[3-(diethylamino)propoxy]-4-chromone

Take 10mmol 7-hydroxy-4-chromone, add 10mmol 3-chloropropyldiethylamine hydrochloride, and 10mmol K ₂ CO ₃ , and 1mmol KI, add an appropriate amount of acetone, reflux for 12 hours, filter with suction, discard Inorganic salts were removed, and the obtained solid was recrystallized after concentrating the solution to obtain white crystal 7-[3-(diethylamino)propoxy]-4-chromone with a yield of 33%. MS m/z (M): 275.34; ¹ HNMR (CDCl ₃ ): δ1.00 (6H, t), 1.81 (1H, m), 2.36 (2H, m), 2.40 (2H, q), 3.94 (2H , m), 6.35 (1H, d, J=9Hz), 6.43 (1H, d, J=3Hz), 6.52 (1H, dd, J1=9Hz d, J2=3Hz), 7.20 (1H, d, J=3Hz) 9Hz), 7.53 (1H, d, J=9Hz).

Example 4: 7-[3-(1-pyrrolidinyl)propoxy]-4-chromone

According to the method of Example 3, white crystal 7-[3-(1-pyrrolidinyl)propoxy]-4-chromone was obtained with a yield of 36%. MS m/z (M): 273.12; ¹ HNMR (CDCl ₃ ): δ1.59 (4H, m), 1.81 (2H, m), 2.25 (4H, q), 2.36 (2H, m), 3.94 (2H , m), 6.35 (1H, d, J=9Hz), 6.43 (1H, d, J=3Hz), 6.52 (1H, dd, J1=9Hz d, J2=3Hz), 7.20 (1H, d, J=3Hz) 9Hz), 7.53 (1H, d, J=9Hz).

Example 5: 7-[3-(1-piperidinyl)propoxy]-4-chromone

According to the method of Example 3, white crystal 7-[3-(1-piperidinyl)propoxy]-4-chromone was obtained with a yield of 38%. MS m/z (M): 287.15; ¹ HNMR (CDCl ₃ ): δ1.50 (6H, m), 1.81 (2H, m), 2.24 (4H, m), 2.36 (2H, m), 3.94 (2H , m), 6.35 (1H, d, J=9Hz), 6.43 (1H, d, J=3Hz), 6.52 (1H, dd, J1=9Hz d, J2=3Hz), 7.20 (1H, d, J=3Hz) 9Hz), 7.53 (1H, d, J=9Hz).

Example 6: 7-[3-(4-morpholino)propoxy]-4-chromone

According to the method of Example 3, white crystal 7-[3-(4-morpholino)propoxy]-4-chromone was obtained with a yield of 40%. MS m/z (M): 289.13; ¹ HNMR (CDCl ₃ ): δ1.81 (2H, m), 2.37 (6H, m), 3.67 (4H, m), 3.94 (2H, m), 6.35 (1H , d, J=9Hz), 6.43(1H, d, J=3Hz), 6.52(1H, dd, J1=9Hz d, J2=3Hz), 7.20(1H, d, J=9Hz), 7.53(1H, d, J=9Hz).

Example 7: 7-[4-(dimethylamino)butoxy]-4-chromone

Take 10mmol of 7-hydroxy-4-chromone, add 10mmol of chlorobutyldimethylamine hydrochloride, 10mmol of K ₂ CO ₃ , and 1mmol of KI, add an appropriate amount of acetone, reflux for 12 hours, filter with suction, and discard the inorganic Salt, after concentrating the solution, the obtained solid was recrystallized to obtain white crystal 7-[4-(dimethylamino)butoxy]-4-chromone with a yield of 47%. MS m/z (M): 261.12; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.71 (2H, m), 2.37 (6H, m), 2.36 (2H, m), 3.94 (2H , m), 6.35 (1H, d, J=9Hz), 6.43 (1H, d, J=3Hz), 6.52 (1H, dd, J1=9Hz d, J2=3Hz), 7.20 (1H, d, J=3Hz) 9Hz), 7.53 (1H, d, J=9Hz).

Example 8: 7-[4-(diethylamino)butoxy]-4-chromone

According to the method in Example 7, white crystal 7-[4-(diethylamino)butoxy]-4-chromone was obtained with a yield of 39%. MS m/z (M): 289.14; ¹ HNMR (CDCl ₃ ): δ1.00 (6H, t), 1.39 (2H, m), 1.71 (2H, m), 2.40 (2H, q), 3.94 (2H , m), 6.35 (1H, d, J=9Hz), 6.43 (1H, d, J=3Hz), 6.52 (1H, dd, J1=9Hz d, J2=3Hz), 7.20 (1H, d, J=3Hz) 9Hz), 7.53 (1H, d, J=9Hz).

Example 9: 7-[4-(1-pyrrolidinyl)butoxy]-4-chromone

According to the method in Example 7, white crystal 7-[4-(1-pyrrolidinyl)butoxy]-4-chromone was obtained with a yield of 28%. MS m/z (M): 287.15; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.59 (4H, m), 1.71 (2H, m), 2.25 (4H, q), 2.36 (2H , m), 3.94 (2H, m), 6.35 (1H, d, J=9Hz), 6.43 (1H, d, J=3Hz), 6.52 (1H, dd, J1=9Hz d, J2=3Hz), 7.20 (1H, d, J=9Hz), 7.53 (1H, d, J=9Hz).

Example 10: 7-[4-(1-piperidinyl)butoxy]-4-chromone

According to the method of Example 7, white crystal 7-[4-(1-piperidinyl)butoxy]-4-chromone was obtained with a yield of 45%. MS m/z (M): 301.17; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.50 (6H, m), 1.71 (2H, m), 2.24 (4H, m), 2.36 (2H , m), 3.94 (2H, m), 6.35 (1H, d, J=9Hz), 6.43 (1H, d, J=3Hz), 6.52 (1H, dd, J1=9Hz d, J2=3Hz), 7.20 (1H, d, J=9Hz), 7.53 (1H, d, J=9Hz).

Example 11: 7-[4-(4-morpholinyl)butoxy]-4-chromone

According to the method in Example 7, white crystal 7-[4-(4-morpholinyl)butoxy]-4-chromone was obtained with a yield of 38%. MS m/z (M): 303.13; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.71 (2H, m), 2.37 (6H, m), 3.67 (4H, m), 3.94 (2H , m), 6.35 (1H, d, J=9Hz), 6.43 (1H, d, J=3Hz), 6.52 (1H, dd, J1=9Hz d, J2=3Hz), 7.20 (1H, d, J=3Hz) 9Hz), 7.53 (1H, d, J=9Hz).

Example 12: 6-[2-(1-pyrrolidinyl)ethoxy]-4-chromone

Take 10mmol of 6-hydroxy-4-chromone, add 10mmol of chloroethylpyrrolidinyl hydrochloride, 10mmol of K ₂ CO ₃ , and 1mmol of KI, add an appropriate amount of acetone, reflux for 12 hours, filter with suction, and discard the inorganic salt, and after concentrating the solution, recrystallize the obtained solid to obtain white crystal 6-[2-(1-pyrrolidinyl)ethoxy]-4-chromone with a yield of 50%. MS m/z (M): 259.12; ¹ HNMR (CDCl ₃ ): δ1.59 (4H, m), 2.25 (4H, q), 2.78 (2H, m), 4.04 (2H, m), 6.35 (1H , d, J=9Hz), 6.81(1H, d, J=9Hz), 6.88(1H, dd, J1=9Hz d, J2=3Hz), 7.15(1H, d, J=3Hz), 7.20(1H, d, J=9Hz).

Example 13: 6-[2-(1-piperidinyl)ethoxy]-4-chromone

According to the method of Example 12, white crystal 6-[2-(1-piperidinyl)ethoxy]-4-chromone was obtained with a yield of 38%. MS m/z (M): 273.33; ¹ HNMR (CDCl ₃ ): δ1.50 (6H, m), 2.24 (4H, m), 2.78 (2H, m), 4.04 (2H, m), 6.35 (1H , d, J=9Hz), 6.81(1H, d, J=9Hz), 6.88(1H, dd, J1=9Hz d, J2=3Hz), 7.15(1H, d, J=3Hz), 7.20(1H, d, J=9Hz).

Example 14: 6-[3-(dimethylamino)propoxy]-4-chromone

Take 10mmol of 6-hydroxy-4-chromone, add 10mmol of chloropropyldimethylamine hydrochloride, 10mmol of K ₂ CO ₃ , and 1mmol of KI, add an appropriate amount of acetone, reflux for 12 hours, filter with suction, and discard the inorganic Salt, after concentrating the solution, recrystallize the obtained solid to obtain white crystal 6-[3-(dimethylamino)propoxy]-4-chromone with a yield of 42%. MS m/z (M): 247.12; ¹ HNMR (CDCl ₃ ): δ1.81 (2H, m), 2.27 (6H, s), 2.36 (2H, m), 3.94 (2H, m), 6.35 (1H , d, J=9Hz), 6.81(1H, d, J=9Hz), 6.88(1H, dd, J1=9Hz d, J2=3Hz), 7.15(1H, d, J=3Hz), 7.20(1H, d, J=9Hz).

Example 15: 6-[3-(diethylamino)propoxy]-4-chromone

According to the method of Example 14, white crystal 6-[3-(diethylamino)propoxy]-4-chromone was obtained with a yield of 39%. MS m/z (M): 275.34; ¹ HNMR (CDCl ₃ ): δ1.00 (6H, t), 1.81 (1H, m), 2.36 (2H, m), 2.40 (2H, q), 3.94 (2H , m), 6.35 (1H, d, J=9Hz), 6.81 (1H, d, J=9Hz), 6.88 (1H, dd, J1=9Hz d, J2=3Hz), 7.15 (1H, d, J= 3Hz), 7.20 (1H, d, J=9Hz).

Example 16: 6-[3-(1-pyrrolidinyl)propoxy]-4-chromone

According to the method of Example 14, white crystal 6-[3-(1-pyridolyl)propoxy]-4-chromone was obtained with a yield of 47%. MS m/z (M): 273.12; ¹ HNMR (CDCl ₃ ): δ1.59 (4H, m), 1.81 (2H, m), 2.25 (4H, q), 2.36 (2H, m), 3.94 (2H , m), 6.35 (1H, d, J=9Hz), 6.81 (1H, d, J=9Hz), 6.88 (1H, dd, J1=9Hz d, J2=3Hz), 7.15 (1H, d, J= 3Hz), 7.20 (1H, d, J=9Hz).

Example 17: 6-[3-(1-piperidinyl)propoxy]-4-chromone

According to the method of Example 14, white crystal 6-[3-(1-piperidinyl)propoxy]-4-chromone was obtained with a yield of 46%. MS m/z (M): 287.15; ¹ HNMR (CDCl ₃ ): δ1.50 (6H, m), 1.81 (2H, m), 2.24 (4H, m), 2.36 (2H, m), 3.94 (2H , m), 6.35 (1H, d, J=9Hz), 6.81 (1H, d, J=9Hz), 6.88 (1H, dd, J1=9Hz d, J2=3Hz), 7.15 (1H, d, J= 3Hz), 7.20 (1H, d, J=9Hz).

Example 18: 6-[3-(4-morpholinyl)propoxy]-4-chromone

According to the method of Example 14, white crystal 6-[3-(4-morpholino)propoxy]-4-chromone was obtained with a yield of 51%. MS m/z (M): 289.13; ¹ HNMR (CDCl ₃ ): δ1.81 (2H, m), 2.37 (6H, m), 3.67 (4H, m), 3.94 (2H, m), 6.35 (1H , d, J=9Hz), 6.81(1H, d, J=9Hz), 6.88(1H, dd, J1=9Hz d, J2=3Hz), 7.15(1H, d, J=3Hz), 7.20(1H, d, J=9Hz).

Example 19: 6-[4-(dimethylamino)butoxy]-4-chromone

Take 10mmol 6-hydroxy-4-chromone, add 10mmol chlorobutyldimethylamine hydrochloride, 10mmol K ₂ CO ₃ , and 1mmol KI, add an appropriate amount of acetone, reflux for 12 hours, filter with suction, and discard the inorganic Salt, after concentrating the solution, the obtained solid was recrystallized to obtain white crystal 6-[4-(dimethylamino)butoxy]-4-chromone with a yield of 45%. MS m/z (M): 261.12; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.71 (2H, m), 2.37 (6H, m), 2.36 (2H, m), 3.94 (2H , m), 6.35 (1H, d, J=9Hz), 6.81 (1H, d, J=9Hz), 6.88 (1H, dd, J1=9Hz d, J2=3Hz), 7.15 (1H, d, J= 3Hz), 7.20 (1H, d, J=9Hz).

Example 20: 6-[4-(diethylamino)butoxy]-4-chromone

According to the method of Example 19, white crystal 6-[4-(diethylamino)butoxy]-4-chromone was obtained with a yield of 42%. MS m/z (M): 289.14; ¹ HNMR (CDCl ₃ ): δ1.00 (6H, t), 1.39 (2H, m), 1.71 (2H, m), 2.40 (2H, q), 3.94 (2H , m), 6.35 (1H, d, J=9Hz), 6.81 (1H, d, J=9Hz), 6.88 (1H, dd, J1=9Hz d, J2=3Hz), 7.15 (1H, d, J= 3Hz), 7.20 (1H, d, J=9Hz).

Example 21: 6-[4-(1-pyrrolidinyl)butoxy]-4-chromone

According to the method in Example 19, white crystals of 6-[4-(1-pyridolyl)butoxy]-4-chromone were obtained with a yield of 47%. MS m/z (M): 287.15; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.59 (4H, m), 1.71 (2H, m), 2.25 (4H, q), 2.36 (2H , m), 3.94 (2H, m), 6.35 (1H, d, J=9Hz), 6.81 (1H, d, J=9Hz), 6.88 (1H, dd, J1=9Hz d, J2=3Hz), 7.15 (1H, d, J=3Hz), 7.20 (1H, d, J=9Hz).

Example 22: 6-[4-(1-piperidinyl)butoxy]-4-chromone

According to the method in Example 19, white crystals of 6-[4-(1-piperidinyl)butoxy]-4-chromone were obtained with a yield of 38%. MS m/z (M): 301.17; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.50 (6H, m), 1.71 (2H, m), 2.24 (4H, m), 2.36 (2H , m), 3.94 (2H, m), 6.35 (1H, d, J=9Hz), 6.81 (1H, d, J=9Hz), 6.88 (1H, dd, J1=9Hz d, J2=3Hz), 7.15 (1H, d, J=3Hz), 7.20 (1H, d, J=9Hz).

Example 23: 6-[4-(4-morpholinyl)butoxy]-4-chromone

According to the method in Example 19, white crystals of 6-[4-(4-morpholinyl)butoxy]-4-chromone were obtained with a yield of 32%. MS m/z (M): 303.13; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.71 (2H, m), 2.37 (6H, m), 3.67 (4H, m), 3.94 (2H , m), 6.35 (1H, d, J=9Hz), 6.81 (1H, d, J=9Hz), 6.88 (1H, dd, J1=9Hz d, J2=3Hz), 7.15 (1H, d, J= 3Hz), 7.20 (1H, d, J=9Hz).

Example 24: 5-[2-(Dimethylamino)ethoxy]-4-chromone

Take 10mmol of 5-hydroxy-4-chromone, add 10mmol of chloroethyldimethylamine hydrochloride, 10mmol of K ₂ CO ₃ , and 1mmol of KI, add an appropriate amount of acetone, reflux for 12 hours, filter with suction, and discard the inorganic Salt, after concentrating the solution, recrystallize the obtained solid to obtain white crystal 5-[2-(dimethylamino)ethoxy]-4-chromone with a yield of 44%. MS m/z (M): 233.37; ¹ HNMR (CDCl ₃ ): δ2.27 (6H, s), 2.78 (2H, m), 4.04 (2H, m), 6.35 (1H, d, J=9Hz) , 6.48 (1H, m), 6.52 (1H, m), 7.20 (1H, m), 7.26 (1H, m).

Example 25: 5-[2-(Diethylamino)ethoxy]-4-chromone

According to the method of Example 24, white crystal 5-[2-(diethylamino)ethoxy]-4-chromone was obtained with a yield of 43%. MS m/z (M): 261.37; ¹ HNMR (CDCl ₃ ): δ1.00 (6H, t), 2.27 (4H, q), 2.78 (2H, m), 4.04 (2H, m), 6.35 (1H , d, J=9Hz), 6.48 (1H, m), 6.52 (1H, m), 7.20 (1H, m), 7.26 (1H, m).

Example 26: 5-[2-(1-pyrrolidinyl)ethoxy]-4-chromone

According to the method of Example 24, white crystal 5-[2-(1-pyrrolidinyl)ethoxy]-4-chromone was obtained with a yield of 45%. MS m/z (M): 259.12; ¹ HNMR (CDCl ₃ ): δ1.59 (4H, m), 2.25 (4H, q), 2.78 (2H, m), 4.04 (2H, m), 6.35 (1H , d, J=9Hz), 6.48 (1H, m), 6.52 (1H, m), 7.20 (1H, m), 7.26 (1H, m).

Example 27: 5-[2-(1-piperidinyl)ethoxy]-4-chromone

According to the method of Example 24, white crystal 5-[2-(1-piperidinyl)ethoxy]-4-chromone was obtained with a yield of 48%. MS m/z (M): 273.33; ¹ HNMR (CDCl ₃ ): δ1.50 (6H, m), 2.24 (4H, m), 2.78 (2H, m), 4.04 (2H, m), 6.35 (1H , d, J=9Hz), 6.48 (1H, m), 6.52 (1H, m), 7.20 (1H, m), 7.26 (1H, m).

Example 28: 5-[2-(4-morpholinyl)ethoxy]-4-chromone

According to the method of Example 24, white crystal 5-[2-(4-morpholinyl)ethoxy]-4-chromone was obtained with a yield of 44%. MS m/z (M): 275.12; ¹ HNMR (CDCl ₃ ): δ2.37 (4H, m), 2.78 (2H, m), 3.67 (4H, m), 4.04 (2H, m), 6.35 (1H , d, J=9Hz), 6.48 (1H, m), 6.52 (1H, m), 7.20 (1H, m), 7.26 (1H, m).

Example 29: 5-[3-(Dimethylamino)propoxy]-4-chromone

Take 10mmol of 5-hydroxy-4-chromone, add 10mmol of chloropropyldimethylamine hydrochloride, 10mmol of K ₂ CO ₃ , and 1mmol of KI, add an appropriate amount of acetone, reflux for 12 hours, filter with suction, and discard the inorganic Salt, after concentrating the solution, recrystallize the obtained solid to obtain white crystal 5-[3-(dimethylamino)propoxy]-4-chromone with a yield of 32%. MS m/z (M): 247.12; ¹ HNMR (CDCl ₃ ): δ1.81 (2H, m), 2.27 (6H, s), 2.36 (2H, m), 3.94 (2H, m), 6.35 (1H , d, J=9Hz), 6.48 (1H, m), 6.52 (1H, m), 7.20 (1H, m), 7.26 (1H, m).

Example 30: 5-[3-(Diethylamino)propoxy]-4-chromone

According to the method of Example 29, white crystal 5-[3-(diethylamino)propoxy]-4-chromone was obtained with a yield of 36%. MS m/z (M): 275.34; ¹ HNMR (CDCl ₃ ): δ1.00 (6H, t), 1.81 (1H, m), 2.36 (2H, m), 2.40 (2H, q), 3.94 (2H , m), 6.35 (1H, d, J=9Hz), 6.48 (1H, m), 6.52 (1H, m), 7.20 (1H, m), 7.26 (1H, m).

Example 31: 5-[3-(1-Pyrrolidinyl)propoxy]-4-chromone

According to the method in Example 29, white crystal 5-[3-(1-pyrrolidinyl)propoxy]-4-chromone was obtained with a yield of 41%. MS m/z (M): 273.12; ¹ HNMR (CDCl ₃ ): δ1.59 (4H, m), 1.81 (2H, m), 2.25 (4H, q), 2.36 (2H, m), 3.94 (2H , m), 6.35 (1H, d, J=9Hz), 6.48 (1H, m), 6.52 (1H, m), 7.20 (1H, m), 7.26 (1H, m).

Example 32: 5-[3-(1-piperidinyl)propoxy]-4-chromone

According to the method in Example 29, white crystal 5-[3-(1-piperidinyl)propoxy]-4-chromone was obtained with a yield of 44%. MS m/z (M): 287.15; ¹ HNMR (CDCl ₃ ): δ1.50 (6H, m), 1.81 (2H, m), 2.24 (4H, m), 2.36 (2H, m), 3.94 (2H , m), 6.35 (1H, d, J=9Hz), 6.48 (1H, m), 6.52 (1H, m), 7.20 (1H, m), 7.26 (1H, m).

Example 33: 5-[3-(4-Morpholinyl)propoxy]-4-chromone

According to the method in Example 29, white crystal 5-[3-(4-morpholinyl)propoxy]-4-chromone was obtained with a yield of 49%. MS m/z (M): 289.13; ¹ HNMR (CDCl ₃ ): δ1.81 (2H, m), 2.37 (6H, m), 3.67 (4H, m), 3.94 (2H, m), 6.35 (1H , d, J=9Hz), 6.48 (1H, m), 6.52 (1H, m), 7.20 (1H, m), 7.26 (1H, m).

Example 34: 5-[4-(Dimethylamino)butoxy]-4-chromone

Take 10mmol of 5-hydroxy-4-chromone, add 10mmol of chlorobutyldimethylamine hydrochloride, 10mmol of K ₂ CO ₃ , and 1mmol of KI, add an appropriate amount of acetone, reflux for 12 hours, filter with suction, and discard the inorganic Salt, after concentrating the solution, the obtained solid was recrystallized to obtain white crystal 5-[4-(dimethylamino)butoxy]-4-chromone with a yield of 28%. MS m/z (M): 261.12; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.71 (2H, m), 2.37 (6H, m), 2.36 (2H, m), 3.94 (2H , m), 6.35 (1H, d, J=9Hz), 6.48 (1H, m), 6.52 (1H, m), 7.20 (1H, m), 7.26 (1H, m).

Example 35: 5-[4-(Diethylamino)butoxy]-4-chromone

According to the method of Example 34, white crystal 5-[4-(diethylamino)butoxy]-4-chromone was obtained with a yield of 26%. MS m/z (M): 289.14; ¹ HNMR (CDCl ₃ ): δ1.00 (6H, t), 1.39 (2H, m), 1.71 (2H, m), 2.40 (2H, q), 3.94 (2H , m), 6.35 (1H, d, J=9Hz), 6.48 (1H, m), 6.52 (1H, m), 7.20 (1H, m), 7.26 (1H, m).

Example 36: 5-[4-(1-Pyrrolidinyl)butoxy]-4-chromone

According to the method of Example 34, white crystal 5-[4-(1-pyrrolidinyl)butoxy]-4-chromone was obtained with a yield of 34%. MS m/z (M): 287.15; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.59 (4H, m), 1.71 (2H, m), 2.25 (4H, q), 2.36 (2H , m), 3.94 (2H, m), 6.35 (1H, d, J=9Hz), 6.48 (1H, m), 6.52 (1H, m), 7.20 (1H, m), 7.26 (1H, m).

Example 37: 5-[4-(1-piperidinyl)butoxy]-4-chromone

According to the method of Example 34, white crystal 5-[4-(1-piperidinyl)butoxy]-4-chromone was obtained with a yield of 38%. MS m/z (M): 301.17; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.50 (6H, m), 1.71 (2H, m), 2.24 (4H, m), 2.36 (2H , m), 3.94 (2H, m), 6.35 (1H, d, J=9Hz), 6.48 (1H, m), 6.52 (1H, m), 7.20 (1H, m), 7.26 (1H, m).

Example 38: 5-[4-(4-Morpholinyl)butoxy]-4-chromone

According to the method of Example 34, white crystal 5-[4-(4-morpholinyl)butoxy]-4-chromone was obtained with a yield of 41%. MS m/z (M): 303.13; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.71 (2H, m), 2.37 (6H, m), 3.67 (4H, m), 3.94 (2H , m), 6.35 (1H, d, J=9Hz), 6.48 (1H, m), 6.52 (1H, m), 7.20 (1H, m), 7.26 (1H, m).

Example 39: 7-[2-(Dimethylamino)ethoxy]-4-chromanone

Take 10mmol 7-hydroxy-4-chromanone, add 10mmol chloroethyldimethylamine hydrochloride, 10mmol K ₂ CO ₃ , and 1mmol KI, add an appropriate amount of acetone, reflux for 12 hours, suction filter, and discard Inorganic salt, after concentrating the solution, recrystallize the obtained solid to obtain white crystal 7-[2-(dimethylamino)ethoxy]-4-chromanone with a yield of 30%. MS m/z (M): 235.12; ¹ HNMR (CDCl ₃ ): δ2.27 (6H, s), 2.78 (2H, m), 2.97 (2H, m), 4.04 (2H, m), 4.13 (2H , m), 6.36 (1H, d, J=3Hz), 6.41 (1H, dd, J1=9Hz d, J2=3Hz), 7.67 (1H, d, J=9Hz).

Example 40: 7-[2-(1-Pyrrolidinyl)ethoxy]-4-chromanone

According to the method in Example 39, white crystals of 7-[2-(1-pyrrolidinyl)ethoxy]-4-chromanone were obtained with a yield of 43%. MS m/z (M): 261.12; ¹ HNMR (CDCl ₃ ): δ1.59 (4H, m), 2.25 (4H, q), 2.78 (2H, m), 2.97 (2H, m), 4.04 (2H , m), 4.13 (2H, m), 6.36 (1H, d, J=3Hz), 6.41 (1H, dd, J1=9Hz d, J2=3Hz), 7.67 (1H, d, J=9Hz).

Example 41: 7-[2-(1-piperidinyl)ethoxy]-4-chromanone

According to the method in Example 39, white crystals of 7-[2-(1-piperidinyl)ethoxy]-4-chromanone were obtained with a yield of 47%. MS m/z (M): 275.33; ¹ HNMR (CDCl ₃ ): δ1.50 (6H, m), 2.24 (4H, m), 2.78 (2H, m), 2.97 (2H, m), 4.04 (2H , m), 4.13 (2H, m), 6.36 (1H, d, J=3Hz), 6.41 (1H, dd, J1=9Hz d, J2=3Hz), 7.67 (1H, d, J=9Hz).

Example 42: 7-[2-(4-Morpholinyl)ethoxy]-4-chromanone

According to the method in Example 39, white crystals of 7-[2-(4-morpholinyl)ethoxy]-4-chromanone were obtained with a yield of 50%. MS m/z (M): 277.12; ¹ HNMR (CDCl ₃ ): δ2.37 (4H, m), 2.78 (2H, m), 2.97 (2H, m), 3.67 (4H, m), 4.04 (2H , m), 4.13 (2H, m), 6.36 (1H, d, J=3Hz), 6.41 (1H, dd, J1=9Hz d, J2=3Hz), 7.67 (1H, d, J=9Hz).

Example 43: 7-[3-(Dimethylamino)propoxy]-4-chromanone

Take 10mmol of 7-hydroxy-4-chromanone, add 10mmol of chloropropyldimethylamine hydrochloride, 10mmol of K ₂ CO ₃ , and 1mmol of KI, add an appropriate amount of acetone, reflux for 12 hours, filter with suction, and discard Inorganic salt, after concentrating the solution, recrystallize the obtained solid to obtain white crystal 7-[3-(dimethylamino)propoxy]-4-chromanone with a yield of 33%. MS m/z (M): 249.12; ¹ HNMR (CDCl ₃ ): δ1.81 (2H, m), 2.27 (6H, s), 2.36 (2H, m), 2.97 (2H, m), 3.94 (2H , m), 4.13 (2H, m), 6.36 (1H, d, J=3Hz), 6.41 (1H, dd, J1=9Hz d, J2=3Hz), 7.67 (1H, d, J=9Hz).

Example 44: 7-[3-(Diethylamino)propoxy]-4-chromanone

According to the method of Example 43, white crystal 7-[3-(diethylamino)propoxy]-4-chromanone was obtained with a yield of 29%. MS m/z (M): 277.34; ¹ HNMR (CDCl ₃ ): δ1.00 (6H, t), 1.81 (1H, m), 2.40 (6H, m), 2.97 (2H, m), 3.94 (2H , m), 4.13 (2H, m), 6.36 (1H, d, J=3Hz), 6.41 (1H, dd, J1=9Hz d, J2=3Hz), 7.67 (1H, d, J=9Hz).

Example 45: 7-[3-(1-Pyrrolidinyl)propoxy]-4-chromanone

According to the method of Example 43, white crystals of 7-[3-(1-pyrrolidinyl)propoxy]-4-chromanone were obtained with a yield of 45%. MS m/z (M): 275.12; ¹ HNMR (CDCl ₃ ): δ1.59 (4H, m), 1.81 (2H, m), 2.25 (4H, m), 2.36 (2H, m), 2.97 (2H , m), 3.94 (2H, m), 6.36 (1H, d, J=3Hz), 6.41 (1H, dd, J1=9Hz d, J2=3Hz), 7.67 (1H, d, J=9Hz).

Example 46: 7-[3-(1-piperidinyl)propoxy]-4-chromanone

According to the method in Example 43, white crystals of 7-[3-(1-piperidinyl)propoxy]-4-chromanone were obtained with a yield of 47%. MS m/z (M): 289.15; ¹ HNMR (CDCl ₃ ): δ1.50 (6H, m), 1.81 (2H, m), 2.24 (4H, m), 2.36 (2H, m), 2.97 (2H , m), 3.94(2H, m), 4.13(2H, m), 6.36 (1H, d, J=3Hz), 6.41(1H, dd, J1=9Hz d, J2=3Hz), 7.67(1H, d , J=9Hz).

Example 47: 7-[3-(4-Morpholinyl)propoxy]-4-chromanone

According to the method of Example 43, white crystals of 7-[3-(4-morpholinyl)propoxy]-4-chromanone were obtained with a yield of 44%. MS m/z (M): 291.13; ¹ HNMR (CDCl ₃ ): δ1.81 (2H, m), 2.37 (6H, m), 2.97 (2H, m), 3.67 (4H, m), 3.94 (2H , m), 4.13 (2H, m), 6.36 (1H, d, J=3Hz), 6.41 (1H, dd, J1=9Hz d, J2=3Hz), 7.67 (1H, d, J=9Hz).

Example 48: 7-[4-(Dimethylamino)butoxy]-4-chromanone

Take 10mmol 7-hydroxy-4-chromanone, add 10mmol chlorobutyldimethylamine hydrochloride, 10mmol K ₂ CO ₃ , and 1mmol KI, add an appropriate amount of acetone, reflux for 12 hours, filter with suction, and discard Inorganic salt, after concentrating the solution, recrystallize the obtained solid to obtain white crystal 7-[4-(dimethylamino)butoxy]-4-chromanone with a yield of 37%. MS m/z (M): 263.12; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.71 (2H, m), 2.27 (2H, s), 2.36 (2H, m), 2.97 (2H , m), 3.94(2H, m), 4.13(2H, m), 6.36 (1H, d, J=3Hz), 6.41(1H, dd, J1=9Hz d, J2=3Hz), 7.67(1H, d , J=9Hz).

Example 49: 7-[4-(Diethylamino)butoxy]-4-chromanone

According to the method of Example 48, white crystal 7-[4-(diethylamino)butoxy]-4-chromanone was obtained with a yield of 34%. MS m/z (M): 291.14; ¹ HNMR (CDCl ₃ ): δ1.00 (6H, t), 1.39 (2H, m), 1.71 (2H, m), 2.40 (6H, q), 2.97 (2H , m), 3.94(2H, m), 4.13(2H, m), 6.36 (1H, d, J=3Hz), 6.41(1H, dd, J1=9Hz d, J2=3Hz), 7.67(1H, d , J=9Hz).

Example 50: 7-[4-(1-Pyrrolidinyl)butoxy]-4-chromanone

According to the method of Example 48, white crystal 7-[4-(1-pyrrolidinyl)butoxy]-4-chromanone was obtained with a yield of 41%. MS m/z (M): 289.15; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.59 (4H, m), 1.71 (2H, m), 2.25 (4H, q), 2.36 (2H , m), 2.97(2H, m), 3.94(2H, m), 4.13(2H, m), 6.36 (1H, d, J=3Hz), 6.41(1H, dd, J1=9Hz d, J2=3Hz ), 7.67 (1H, d, J=9Hz).

Example 51: 7-[4-(1-Piperidinyl)butoxy]-4-chromanone

According to the method in Example 48, white crystals of 7-[4-(1-piperidinyl)butoxy]-4-chromanone were obtained with a yield of 48%. MS m/z (M): 303.17; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.50 (6H, m), 1.71 (2H, m), 2.24 (4H, m), 2.36 (2H , m), 2.97(2H, m), 3.94(2H, m), 4.13(2H, m), 6.36 (1H, d, J=3Hz), 6.41(1H, dd, J1=9Hz d, J2=3Hz ), 7.67 (1H, d, J=9Hz).

Example 52: 7-[4-(4-Morpholinyl)butoxy]-4-chromanone

According to the method in Example 48, white crystals of 7-[4-(4-morpholinyl)butoxy]-4-chromanone were obtained with a yield of 42%. MS m/z (M): 305.13; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.71 (2H, m), 2.37 (6H, m), 2.97 (2H, m), 3.67 (4H , m), 3.94(2H, m), 4.13(2H, m), 6.36 (1H, d, J=3Hz), 6.41(1H, dd, J1=9Hz d, J2=3Hz), 7.67(1H, d , J=9Hz).

Example 53: 6-[2-(Dimethylamino)ethoxy]-4-chromanone

Take 10mmol of 6-hydroxy-4-chromanone, add 10mmol of chloroethyldimethylamine hydrochloride, 10mmol of K ₂ CO ₃ , and 1mmol of KI, add an appropriate amount of acetone, reflux for 12 hours, filter with suction, and discard Inorganic salt, after concentrating the solution, recrystallize the obtained solid to obtain white crystal 6-[2-(dimethylamino)ethoxy]-4-chromanone with a yield of 27%. MS m/z (M): 235.37; ¹ HNMR (CDCl ₃ ): δ2.27 (6H, s), 2.78 (2H, m), 2.97 (2H, m), 4.04 (2H, m), 4.04 (2H , m), 6.74 (1H, d, J=9Hz), 6.84 (1H, dd, J1=9Hz d, J2=3Hz), 7.29 (1H, d, J=3Hz).

Example 54: 6-[2-(Diethylamino)ethoxy]-4-chromanone

According to the method of Example 53, white crystals of 6-[2-(diethylamino)ethoxy]-4-chromanone were obtained with a yield of 31%. MS m/z (M): 263.37; ¹ HNMR (CDCl ₃ ): δ1.00 (6H, t), 2.40 (4H, q), 2.78 (2H, m), 2.97 (2H, m), 4.04 (2H , m), 4.13 (2H, m), 6.74 (1H, d, J=9Hz), 6.84 (1H, dd, J1=9Hz d, J2=3Hz), 7.29 (1H, d, J=3Hz).

Example 55: 6-[2-(1-Pyrrolidinyl)ethoxy]-4-chromanone

According to the method of Example 53, white crystals of 6-[2-(1-pyrrolidinyl)ethoxy]-4-chromanone were obtained with a yield of 40%. MS m/z (M): 261.12; ¹ HNMR (CDCl ₃ ): δ1.59 (4H, m), 2.25 (4H, q), 2.78 (2H, m), 2.97 (2H, m), 4.04 (2H , m), 4.13 (2H, m), 6.74 (1H, d, J=9Hz), 6.84 (1H, dd, J1=9Hz d, J2=3Hz), 7.29 (1H, d, J=3Hz).

Example 56: 6-[2-(1-Piperidinyl)ethoxy]-4-chromanone

According to the method in Example 53, white crystals of 6-[2-(1-piperidinyl)ethoxy]-4-chromanone were obtained with a yield of 43%. MS m/z (M): 275.33; ¹ HNMR (CDCl ₃ ): δ1.50 (6H, m), 2.24 (4H, m), 2.78 (2H, m), 2.97 (2H, m), 4.04 (2H , m), 4.13 (2H, m), 6.74 (1H, d, J=9Hz), 6.84 (1H, dd, J1=9Hz d, J2=3Hz), 7.29 (1H, d, J=3Hz).

Example 57: 6-[2-(4-Morpholinyl)ethoxy]-4-chromanone

According to the method of Example 53, white crystals of 6-[2-(4-morpholinyl)ethoxy]-4-chromanone were obtained with a yield of 47%. MS m/z (M): 277.12; ¹ HNMR (CDCl ₃ ): δ2.37 (4H, m), 2.78 (2H, m), 2.97 (2H, m), 3.67 (4H, m), 4.04 (2H , m), 4.13 (2H, m), 6.74 (1H, d, J=9Hz), 6.84 (1H, dd, J1=9Hz d, J2=3Hz), 7.29 (1H, d, J=3Hz).

Example 58: 6-[3-(Dimethylamino)propoxy]-4-chromanone

Take 10mmol 6-hydroxy-4-chromanone, add 10mmol chloropropyldimethylamine hydrochloride, 10mmol K ₂ CO ₃ , and 1mmol KI, add appropriate amount of acetone, reflux for 12 hours, suction filter, discard Inorganic salt, after concentrating the solution, recrystallize the obtained solid to obtain white crystal 6-[3-(dimethylamino)propoxy]-4-chromanone with a yield of 34%. MS m/z (M): 249.12; ¹ HNMR (CDCl ₃ ): δ1.81 (2H, m), 2.27 (6H, s), 2.36 (2H, m), 2.97 (2H, m), 3.94 (2H , m), 4.13 (2H, m), 6.74 (1H, d, J=9Hz), 6.84 (1H, dd, J1=9Hz d, J2=3Hz), 7.29 (1H, d, J=3Hz).

Example 59: 6-[3-(Diethylamino)propoxy]-4-chromanone

According to the method in Example 58, white crystals of 6-[3-(diethylamino)propoxy]-4-chromanone were obtained with a yield of 32%. MS m/z (M): 277.34; ¹ HNMR (CDCl ₃ ): δ1.00 (6H, t), 1.81 (1H, m), 2.36 (2H, m), 2.40 (2H, q), 2.97 (2H , m), 3.94(2H, m), 4.13(2H, m), 6.74 (1H, d, J=9Hz), 6.84(1H, dd, J1=9Hz d, J2=3Hz), 7.29(1H, d , J=3Hz).

Example 60: 6-[3-(1-Pyrrolidinyl)propoxy]-4-chromanone

According to the method of Example 58, white crystals of 6-[3-(1-pyrrolidinyl)propoxy]-4-chromanone were obtained with a yield of 41%. MS m/z (M): 275.12; ¹ HNMR (CDCl ₃ ): δ1.59 (4H, m), 1.81 (2H, m), 2.25 (4H, q), 2.36 (2H, m), 2.97 (2H , m), 3.94(2H, m), 4.13(2H, m), 6.74 (1H, d, J=9Hz), 6.84(1H, dd, J1=9Hz d, J2=3Hz), 7.29(1H, d , J=3Hz).

Example 61: 6-[3-(1-Piperidinyl)propoxy]-4-chromanone

According to the method of Example 58, white crystals of 6-[3-(1-piperidinyl)propoxy]-4-chromanone were obtained with a yield of 44%. MS m/z (M): 289.15; ¹ HNMR (CDCl ₃ ): δ1.50 (6H, m), 1.81 (2H, m), 2.24 (4H, m), 2.36 (2H, m), 2.97 (2H , m), 3.94(2H, m), 4.13(2H, m), 6.74 (1H, d, J=9Hz), 6.84(1H, dd, J1=9Hz d, J2=3Hz), 7.29(1H, d , J=3Hz).

Example 62: 6-[3-(4-Morpholinyl)propoxy]-4-chromanone

According to the method of Example 58, white crystals of 6-[3-(4-morpholinyl)propoxy]-4-chromanone were obtained with a yield of 42%. MS m/z (M): 291.13; ¹ HNMR (CDCl ₃ ): δ1.81 (2H, m), 2.37 (6H, m), 2.97 (2H, m), 3.67 (4H, m), 3.94 (2H , m), 4.13 (2H, m), 6.74 (1H, d, J=9Hz), 6.84 (1H, dd, J1=9Hz d, J2=3Hz), 7.29 (1H, d, J=3Hz).

Example 63: 6-[4-(Dimethylamino)butoxy]-4-chromanone

Take 10mmol of 6-hydroxy-4-chromanone, add 10mmol of chlorobutyldimethylamine hydrochloride, 10mmol of K ₂ CO ₃ , and 1mmol of KI, add an appropriate amount of acetone, reflux for 12 hours, filter with suction, and discard Inorganic salt, after concentrating the solution, recrystallize the obtained solid to obtain white crystal 6-[4-(dimethylamino)butoxy]-4-chromanone with a yield of 31%. MS m/z (M): 263.12; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.71 (2H, m), 2.37 (6H, m), 2.36 (2H, m), 2.97 (2H , m), 3.94(2H, m), 4.13(2H, m), 6.74 (1H, d, J=9Hz), 6.84(1H, dd, J1=9Hz d, J2=3Hz), 7.29(1H, d , J=3Hz).

Example 64: 6-[4-(Diethylamino)butoxy]-4-chromanone

According to the method of Example 63, white crystals of 6-[4-(diethylamino)butoxy]-4-chromanone were obtained with a yield of 35%. MS m/z (M): 291.14; ¹ HNMR (CDCl ₃ ): δ1.00 (6H, t), 1.39 (2H, m), 1.71 (2H, m), 2.40 (2H, q), 2.97 (2H , m), 3.94(2H, m), 4.13(2H, m), 6.74 (1H, d, J=9Hz), 6.84(1H, dd, J1=9Hz d, J2=3Hz), 7.29(1H, d , J=3Hz).

Example 65: 6-[4-(1-Pyrrolidinyl)butoxy]-4-chromanone

According to the method of Example 63, white crystals of 6-[4-(1-pyrrolidinyl)butoxy]-4-chromanone were obtained with a yield of 41%. MS m/z (M): 289.15; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.59 (4H, m), 1.71 (2H, m), 2.25 (4H, q), 2.36 (2H , m), 2.97(2H, m), 3.94(2H, m), 4.13(2H, m), 6.74 (1H, d, J=9Hz), 6.84(1H, dd, J1=9Hz d, J2=3Hz ), 7.29 (1H, d, J=3Hz).

Example 66: 6-[4-(1-Piperidinyl)butoxy]-4-chromanone

According to the method of Example 63, white crystals of 6-[4-(1-piperidinyl)butoxy]-4-chromanone were obtained with a yield of 43%. MS m/z (M): 303.17; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.50 (6H, m), 1.71 (2H, m), 2.24 (4H, m), 2.36 (2H , m), 2.97(2H, m), 3.94(2H, m), 4.13(2H, m), 6.74 (1H, d, J=9Hz), 6.84(1H, dd, J1=9Hz d, J2=3Hz ), 7.29 (1H, d, J=3Hz).

Example 67: 6-[4-(4-Morpholinyl)butoxy]-4-chromanone

According to the method of Example 63, white crystals of 6-[4-(4-morpholinyl)butoxy]-4-chromanone were obtained with a yield of 49%. MS m/z (M): 305.13; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.71 (2H, m), 2.37 (6H, m), 2.97 (2H, m), 3.67 (4H , m), 3.94(2H, m), 4.13(2H, m), 6.74 (1H, d, J=9Hz), 6.84(1H, dd, J1=9Hz d, J2=3Hz), 7.29(1H, d , J=3Hz).

Example 68: 5-[2-(Dimethylamino)ethoxy]-4-chromanone

Take 10mmol of 5-hydroxy-4-chromanone, add 10mmol of chloroethyldimethylamine hydrochloride, 10mmol of K ₂ CO ₃ , and 1mmol of KI, add an appropriate amount of acetone, reflux for 12 hours, filter with suction, and discard Inorganic salt, after concentrating the solution, recrystallize the obtained solid to obtain white crystal 5-[2-(dimethylamino)ethoxy]-4-chromanone with a yield of 24%. MS m/z (M): 235.37; ¹ HNMR (CDCl ₃ ): δ2.27 (6H, s), 2.78 (2H, m), 2.97 (2H, m), 4.04 (2H, m), 4.13 (2H , m), 6.41 (2H, m), 7.22 (1H, m).

Example 69: 5-[2-(Diethylamino)ethoxy]-4-chromanone

According to the method of Example 68, white crystal 5-[2-(diethylamino)ethoxy]-4-chromanone was obtained with a yield of 29%. MS m/z (M): 263.37; ¹ HNMR (CDCl ₃ ): δ1.00 (6H, t), 2.27 (4H, q), 2.78 (2H, m), 2.97 (2H, m), 4.04 (2H , m), 4.13 (2H, m), 6.41 (2H, m), 7.22 (1H, m).

Example 70: 5-[2-(1-Pyrrolidinyl)ethoxy]-4-chromanone

According to the method of Example 68, white crystals of 5-[2-(1-pyrrolidinyl)ethoxy]-4-chromanone were obtained with a yield of 38%. MS m/z (M): 261.12; ¹ HNMR (CDCl ₃ ): δ1.59 (4H, m), 2.25 (4H, q), 2.78 (2H, m), 2.97 (2H, m), 4.04 (2H , m), 4.13 (2H, m), 6.41 (2H, m), 7.22 (1H, m).

Example 71: 5-[2-(1-Piperidinyl)ethoxy]-4-chromanone

According to the method of Example 68, white crystals of 5-[2-(1-piperidinyl)ethoxy]-4-chromanone were obtained with a yield of 44%. MS m/z (M): 275.33; ¹ HNMR (CDCl ₃ ): δ1.50 (6H, m), 2.24 (4H, m), 2.78 (2H, m), 2.97 (2H, m), 4.04 (2H , m), 4.13 (2H, m), 6.41 (2H, m), 7.22 (1H, m).

Example 72: 5-[2-(4-Morpholinyl)ethoxy]-4-chromanone

According to the method of Example 68, white crystals of 5-[2-(4-morpholinyl)ethoxy]-4-chromanone were obtained with a yield of 47%. MS m/z (M): 277.12; ¹ HNMR (CDCl ₃ ): δ2.37 (4H, m), 2.78 (2H, m), 2.97 (2H, m), 3.67 (4H, m), 4.04 (2H , m), 4.13 (2H, m), 6.41 (2H, m), 7.22 (1H, m).

Example 73: 5-[3-(Dimethylamino)propoxy]-4-chromanone

Take 10mmol of 5-hydroxy-4-chromanone, add 10mmol of chloropropyldimethylamine hydrochloride, 10mmol of K ₂ CO ₃ , and 1mmol of KI, add an appropriate amount of acetone, reflux for 12 hours, filter with suction, and discard Inorganic salt, after concentrating the solution, recrystallize the obtained solid to obtain white crystal 5-[3-(dimethylamino)propoxy]-4-chromanone with a yield of 30%. MS m/z (M): 249.12; ¹ HNMR (CDCl ₃ ): δ1.81 (2H, m), 2.27 (6H, s), 2.36 (2H, m), 2.97 (2H, m), 3.94 (2H , m), 4.13 (2H, m), 6.41 (2H, m), 7.22 (1H, m).

Example 74: 5-[3-(Diethylamino)propoxy]-4-chromanone

According to the method of Example 73, white crystals of 5-[3-(diethylamino)propoxy]-4-chromanone were obtained with a yield of 37%. MS m/z (M): 277.34; ¹ HNMR (CDCl ₃ ): δ1.00 (6H, t), 1.81 (1H, m), 2.36 (2H, m), 2.40 (2H, q), 2.97 (2H , m), 3.94 (2H, m), 4.13 (2H, m), 6.41 (2H, m), 7.22 (1H, m).

Example 75: 5-[3-(1-Pyrrolidinyl)propoxy]-4-chromanone

According to the method of Example 73, white crystals of 5-[3-(1-pyrrolidinyl)propoxy]-4-chromanone were obtained with a yield of 44%. MS m/z (M): 275.12; ¹ HNMR (CDCl ₃ ): δ1.59 (4H, m), 1.81 (2H, m), 2.25 (4H, q), 2.36 (2H, m), 2.97 (2H , m), 3.94 (2H, m), 4.13 (2H, m), 6.35 (2H, m), 7.22 (1H, m).

Example 76: 5-[3-(1-Piperidinyl)propoxy]-4-chromanone

According to the method of Example 73, white crystals of 5-[3-(1-piperidinyl)propoxy]-4-chromanone were obtained with a yield of 47%. MS m/z (M): 289.15; ¹ HNMR (CDCl ₃ ): δ1.50 (6H, m), 1.81 (2H, m), 2.24 (4H, m), 2.36 (2H, m), 2.97 (2H , m), 3.94 (2H, m), 4.13 (2H, m), 6.41 (2H, m), 7.22 (1H, m).

Example 77: 5-[3-(4-Morpholinyl)propoxy]-4-chromanone

According to the method of Example 73, white crystals of 5-[3-(4-morpholinyl)propoxy]-4-chromanone were obtained with a yield of 50%. MS m/z (M): 291.13; ¹ HNMR (CDCl ₃ ): δ1.81 (2H, m), 2.37 (6H, m), 2.97 (2H, m), 3.67 (4H, m), 3.94 (2H , m), 4.13 (2H, m), 6.41 (2H, d, J=9Hz), 7.22 (1H, m).

Example 78: 5-[4-(Dimethylamino)butoxy]-4-chromanone

Take 10mmol 5-hydroxy-4-chromanone, add 10mmol chlorobutyldimethylamine hydrochloride, 10mmol K ₂ CO ₃ , and 1mmol KI, add an appropriate amount of acetone, reflux for 12 hours, suction filter, and discard Inorganic salt, after concentrating the solution, recrystallize the obtained solid to obtain white crystal 5-[4-(dimethylamino)butoxy]-4-chromanone with a yield of 31%. MS m/z (M): 263.12; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.71 (2H, m), 2.37 (6H, m), 2.36 (2H, m), 2.97 (2H , m), 3.94 (2H, m), 4.13 (2H, m), 6.41 (2H, m), 7.22 (1H, m).

Example 79: 5-[4-(Diethylamino)butoxy]-4-chromanone

According to the method of Example 78, white crystal 5-[4-(diethylamino)butoxy]-4-chromanone was obtained with a yield of 34%. MS m/z (M): 291.14; ¹ HNMR (CDCl ₃ ): δ1.00 (6H, t), 1.39 (2H, m), 1.71 (2H, m), 2.40 (2H, q), 2.97 (2H , m), 3.94 (2H, m), 4.13 (2H, m), 6.41 (2H, m), 7.22 (1H, m).

Example 80: 5-[4-(1-Pyrrolidinyl)butoxy]-4-chromanone

According to the method of Example 78, white crystals of 5-[4-(1-pyrrolidinyl)butoxy]-4-chromanone were obtained with a yield of 41%. MS m/z (M): 289.15; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.59 (4H, m), 1.71 (2H, m), 2.25 (4H, q), 2.36 (2H , m), 2.97 (2H, m), 3.94 (2H, m), 4.13 (2H, m), 6.41 (2H, m), 7.22 (1H, m).

Example 81: 5-[4-(1-Piperidinyl)butoxy]-4-chromanone

According to the method of Example 78, white crystals of 5-[4-(1-piperidinyl)butoxy]-4-chromanone were obtained with a yield of 48%. MS m/z (M): 303.17; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.50 (6H, m), 1.71 (2H, m), 2.24 (4H, m), 2.36 (2H , m), 2.97 (2H, m), 3.94 (2H, m), 4.13 (2H, m), 6.41 (2H, m), 7.22 (1H, m).

Example 82: 5-[4-(4-Morpholinyl)butoxy]-4-chromanone

According to the method of Example 78, white crystals of 5-[4-(4-morpholinyl)butoxy]-4-chromanone were obtained with a yield of 42%. MS m/z (M): 305.13; ¹ HNMR (CDCl ₃ ): δ1.39 (2H, m), 1.71 (2H, m), 2.37 (6H, m), 2.97 (2H, m), 3.67 (4H , m), 3.94 (2H, m), 4.13 (2H, m), 6.41 (2H, d, J=9Hz), 7.22 (1H, m).

Example 83: 7-(Dimethylamino)formylmethoxychroman

Take 1mmol 7-hydroxychroman, add 1mmol chloroacetyldimethylamine, 10mmol K ₂ CO ₃ , and 0.1mmol KI, add appropriate amount of acetone, reflux for 12 hours, filter with suction, discard inorganic salts, concentrate the solution The obtained solid was recrystallized to obtain a white solid 7-(dimethylamino)formylmethoxychroman with a yield of 40%. MS m/z (M): 219.28. ¹ HNMR (CDCl ₃ ): δ2.04(2H, m), 2.55(2H, t), 2.90(6H, s), 3.94(2H, t), 4.83(2H, s), 6.23 (1H, d) , 6.28 (1H, dd), 6.90 (1H, dd).

Example 84: 7-(Diethylamino)formylmethoxychroman

According to the method of Example 83, 7-hydroxychroman was reacted with chloroacetyldiethylamine to obtain white crystal 7-(diethylamino)formylmethoxychroman with a yield of 51%. MS m/z (M): 247.33. ¹ HNMR (CDCl ₃ ): δ1.44 (6H, m), 2.04 (2H, m), 2.55 (2H, t), 3.24 (4H, m), 3.94 (2H, t), 4.83 (2H, s) , 6.23 (1H, d), 6.28 (1H, dd), 6.90 (1H, dd).

Example 85: 7-(1-Pyrrolidinyl)formylmethoxychroman

According to the method of Example 83, 7-(1-pyrrolidinyl)formylmethoxychroman was reacted with 7-hydroxychroman and N-chloroacetylpyrrole to obtain white crystals with a yield of 53%. MS m/z (M): 241.29. ¹ HNMR (CDCl ₃ ): δ1.97 (4H, m), 2.04 (2H, m), 2.55 (2H, t), 3.46 (4H, m), 3.94 (2H, t), 4.83 (2H, s) , 6.23 (1H, d), 6.28 (1H, dd), 6.90 (1H, dd).

Example 86: 7-(1-Piperidinyl)formylmethoxychroman

According to the method of Example 83, 7-hydroxychroman was reacted with N-chloroacetylpiperidine to obtain white crystal 7-(1-piperidinyl)formylmethoxychroman with a yield of 52%. MS m/z (M): 259.34. ¹ HNMR (CDCl ₃ ): δ1.50 (6H, m), 2.04 (2H, m), 2.55 (2H, t), 3.34 (4H, m), 3.94 (2H, t), 4.83 (2H, s) , 6.23 (1H, d), 6.28 (1H, dd), 6.90 (1H, dd).

Example 87: 7-(4-Morpholinyl)formylmethoxychroman

According to the method of Example 83, 7-hydroxychroman was reacted with N-chloroacetylmorpholine to obtain white crystal 7-(4-morpholino)formylmethoxychroman with a yield of 53%. MS m/z (M): 261.32. ¹ HNMR (CDCl ₃ ): δ2.04(2H, m), 2.55(2H, t), 3.47(4H, m), 3.67(4H, m), 3.94(2H, t), 4.83(2H, s) , 6.23 (1H, d), 6.28 (1H, dd), 6.90 (1H, dd).

Example 88: 6-(Dimethylamino)formylmethoxychroman

Take 1mmol 6-hydroxychroman, add 1mmol chloroacetyldimethylamine, 10mmol K ₂ CO ₃ , and 0.1mmol KI, add appropriate amount of acetone, reflux for 12 hours, filter with suction, discard inorganic salts, concentrate the solution The obtained solid was recrystallized to obtain 6-(dimethylamino)formylmethoxychroman as a white solid with a yield of 30%. MS m/z (M): 219.28. ¹ HNMR (CDCl ₃ ): δ2.04 (2H, m), 2.55 (2H, t), 2.90 (6H, s), 3.94 (2H, t), 4.83 (2H, s), 6.48 (1H, dd) , 6.52 (1H, d), 6.61 (1H, dd).

Example 89: 6-(Diethylamino)formylmethoxychroman

According to the method of Example 88, 6-hydroxychroman was reacted with chloroacetyldiethylamine to obtain 6-(diethylamino)formylmethoxychroman as a white solid with a yield of 52%. MS m/z (M): 247.33. ¹ HNMR (CDCl ₃ ): δ1.44 (6H, m), 2.04 (2H, m), 2.55 (2H, t), 3.26 (4H, m), 3.94 (2H, t), 4.83 (2H, s) , 6.48 (1H, dd), 6.52 (1H, d), 6.61 (1H, dd).

Example 90: 6-(1-Pyrrolidinyl)formylmethoxychroman

According to the method of Example 88, 6-hydroxychroman was reacted with N-chloroacetylpyrrole to obtain 6-(1-pyrrolidinyl)formylmethoxychroman as a white solid with a yield of 50%. MS m/z (M): 241.29. ¹ HNMR (CDCl ₃ ): δ1.97 (4H, m), 2.04 (2H, m), 2.55 (2H, t), 3.46 (4H, m), 3.94 (2H, t), 4.83 (2H, s) , 6.48 (1H, dd), 6.52 (1H, d), 6.61 (1H, dd).

Example 91: 6-(1-Piperidinyl)formylmethoxychroman

According to the method of Example 88, 6-hydroxychroman was reacted with N-chloroacetylpiperidine to obtain 6-(1-piperidinyl)formylmethoxychroman as a white solid with a yield of 47%. MS m/z (M): 259.34. ¹ HNMR (CDCl ₃ ): δ1.50 (6H, m), 2.04 (2H, m), 2.55 (2H, t), 3.26 (4H, m), 3.94 (2H, t), 4.83 (2H, s) , 6.48 (1H, dd), 6.52 (1H, d), 6.61 (1H, dd).

Example 92: 6-(4-Morpholinyl)formylmethoxychroman

According to the method of Example 88, 6-hydroxychroman was reacted with N-chloroacetylmorpholine to obtain 6-(4-morpholino)formylmethoxychroman as a white solid with a yield of 51%. MS m/z (M): 261.32. ¹ HNMR (CDCl ₃ ): δ2.04(2H, m), 2.55(2H, t), 3.47(4H, m), 3.67(4H, m), 3.94(2H, t), 4.83(2H, s) , 6.48 (1H, dd), 6.52 (1H, d), 6.61 (1H, dd).

Example 93: 5-(Dimethylamino)formylmethoxychroman

Take 1mmol of 5-hydroxychroman, add 1mmol of chloroacetyldimethylamine, 10mmol of K ₂ CO ₃ , and 0.1mmol of KI, add an appropriate amount of acetone, reflux for 12 hours, filter with suction, discard inorganic salts, concentrate the solution, and The obtained solid was recrystallized to obtain a white solid 5-(dimethylamino)formylmethoxychroman with a yield of 34%. MS m/z (M): 219.28. ¹ HNMR (CDCl ₃ ): δ2.04 (2H, m), 2.27 (6H, s), 2.55 (2H, t), 3.94 (2H, m), 4.83 (2H, s), 6.28 (2H, m) , 6.86 (1H, m).

Example 94: 5-(Diethylamino)formylmethoxychroman

According to the method of Example 93, 5-hydroxychroman was reacted with chloroacetyldiethylamine to obtain 5-(diethylamino)formylmethoxychroman as a white solid with a yield of 35%. MS m/z (M): 247.33. ¹ HNMR (CDCl ₃ ): δ1.44 (6H, m), 2.04 (2H, m), 2.40 (4H, m), 2.55 (2H, t), 3.94 (2H, m), 4.83 (2H, s) , 6.28 (2H, m), 6.86 (1H, m).

Example 95: 5-(1-Pyrrolidinyl)formylmethoxychroman

According to the method of Example 93, 5-hydroxychroman was reacted with N-chloroacetylpyrrole to obtain 5-(1-pyrrolidinyl)formylmethoxychroman as a white solid with a yield of 53%. MS m/z (M): 241.29. ¹ HNMR (CDCl ₃ ): δ1.59 (4H, m), 2.04 (2H, m), 2.25 (4H, m), 2.55 (2H, t), 3.94 (2H, m), 4.83 (2H, s) , 6.28 (2H, m), 6.86 (1H, m).

Example 96: 5-(1-Piperidinyl)formylmethoxychroman

According to the method of Example 93, 5-hydroxychroman was reacted with N-chloroacetylpiperidine to obtain 5-(1-piperidinyl)formylmethoxychroman as a white solid with a yield of 55%. MS m/z (M): 259.34. ¹ HNMR (CDCl ₃ ): δ1.50 (6H, m), 2.04 (2H, m), 2.24 (4H, m), 2.55 (2H, t), 3.94 (2H, m), 4.83 (2H, s) , 6.28 (2H, m), 6.86 (1H, m).

Example 97: 5-(4-Morpholinyl)formylmethoxychroman

According to the method of Example 93, 5-hydroxychroman was reacted with N-chloroacetylmorpholine to obtain 5-(4-morpholino)formylmethoxychroman as a white solid with a yield of 52%. MS m/z (M): 261.32. ¹ HNMR (CDCl ₃ ): δ2.04 (2H, m), 2.37 (4H, m), 2.55 (2H, t), 3.67 (4H, m), 3.94 (2H, m), 4.83 (2H, s) , 6.28 (2H, m), 6.86 (1H, m).

Example 98: Tablets

components Mass (mg/tablet) active ingredient 2.5-1000 microcrystalline cellulose 200-650 silica 10-650 stearic acid 5-15

  Mix the active ingredient with the microcrystalline cellulose in an equal amount and increase, then add silicon dioxide and stearic acid, mix well, and directly compress the tablet.

Example 99: Tablets

components Mass (mg/tablet) active ingredient 25-1000 starch 45 microcrystalline cellulose 35 Polyvinylpyrrolidone (as a 10% aqueous solution) 4 Sodium carboxymethyl cellulose 4.5 Magnesium stearate 0.5 talc 1

Pass the active ingredient, starch and cellulose through a 45-mesh U.S. sieve and mix thoroughly. The resulting powder is mixed with polyvinylpyrrolidone and then passed through a 14-mesh U.S. sieve. The granules thus obtained are dried at 50-60° C. and passed through a 18-mesh U.S. screen. The sodium carboxymethylcellulose, magnesium stearate and talc, first passed through a 60 mesh U.S. sieve, are then added to the above granules, mixed and compressed on a tablet machine to form tablets.

Example 100: Suspension Concentrate

components Mass (mg/5mL) active ingredient 0.1-1000mg Sodium carboxymethyl cellulose 50mg syrup 1.25mg Benzoic acid solution 0.1mL flavoring agent Appropriate amount Colorant Appropriate amount Add pure water to 5mL

Pass the drug through a 45 mesh U.S. sieve and mix with sodium carboxymethylcellulose and syrup to form a homogeneous paste. Dilute the benzoic acid solution, flavor, and color with some water and add with stirring, then add Sufficient water to achieve desired volume.

Example 101: Aerosol

components Mass (wt%) active ingredient 0.25 ethanol 25.75 Propellant 22 (chlorodifluoromethane) 70

The active ingredient was mixed with ethanol, and the resulting mixture was added to a portion of the propellant 22, cooled to 30°C, and transferred to a container. The required amount is then added to the stainless steel container and diluted with the remainder of the propellant before installing the valve assembly.

Example 102: Suppositories

components Mass (mg/suppository) active ingredient 250 Saturated Fatty Acid Glycerides 2000

The active ingredient is passed through a No. 60 mesh U.S. sieve and suspended in pre-melted saturated fatty acid glycerides. The mixture is then poured into standard 2g cavity suppository molds and allowed to cool.

Example 103: Injectable Formulation

components quality active ingredient 50mg isotonic saline solution 1000mL

The above solution was administered intravenously to the patient at a rate of about 1 mL per minute.

Example 104: Biological activity test - in vitro inhibition of purified enzymes

Reagents: All buffer salts were obtained from Sigma in the highest purity. The hydrolyzate is N-benzoyl-Phe-Val-Arg-p-nitro-anilide (Sigma B7632), N-benzoyl-Ile-Glu-Gly-Arg-p-nitro-anilide hydrochloride ( Sigma B2291), N-p-toluenesulfonyl-Gly-Pro-Lys-p-nitroanilide (Sigma T6140), N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide ( Sigma S7388) and N-CBZ-Val-Gly-Arg-p-nitroanilide (Sigma C7271), purchased from Sigma. N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide (BACHEM L-1720) and N-succinyl-Ala-Ala-Pro-Val-p-nitroanilide (BACHEM L-1720) -1770) were purchased from BACHEM.

Human α-thrombin, human factor Xa and human plasmin were purchased from Enzyme Research Laboratories (South Bend, Indiana).

Ki determination: All determinations are based on the ability of the test compound to inhibit the enzyme-catalyzed hydrolysis of the peptide p-nitroanilide hydrolyzate. In a typical Ki assay, digests are prepared in DMSO and diluted in assay buffer consisting of 50 mM HEPES, 200 mM NaCl, pH 7.5. The final concentration of each digest is listed below. Generally speaking, the concentration of the hydrolyzate is lower than the Km value determined by the experimental method. The test compound was made into 1.0 mg/ml DMSO solution. Prepare DMSO dilutions at 3 final concentrations spanning up to 200-fold. Prepare enzyme solutions in assay buffer at the following concentrations.

In a typical Ki assay, 280 mL of enzyme digest solution, 10 mL of test compound solution is pipetted into each well of a 96-well plate, and the plate is thermally equilibrated in a Molecular Devices plate reader at 37°C for over 15 minutes. A 10 mL aliquot of enzyme was added to initiate the reaction and the increase in absorbance was recorded at 405 nm over 15 min. Data corresponding to hydrolysis of less than 10% of the total lysates were used for calculations. The velocity ratio (rate of change in absorbance, as a function of time) of the sample without the test compound divided by the velocity of the sample containing the test compound is plotted as a function of the test compound trial. Perform linear regression on the data to calculate the slope value of the line. The reciprocal of the slope is the experimentally determined Ki value.

Thrombin: The criterion for evaluating thrombin activity is the ability to hydrolyze the enzymatic hydrolyzate N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide. Prepare enzymatic hydrolyzate solution in assay buffer, concentration is 32Mm (32mM is far less than Km 180mM). The final DMSO concentration was 4.3%. Purified human α-thrombin was diluted to a concentration of 15 nM in assay buffer. The final reagent concentrations are: (thrombin)=0.5nM, (enzyme hydrolyzate N-succinyl-Ala-Ala-Pro-Arg-p-nitroanilide)=32mM.

Factor X (FXa): The standard for evaluating FXa activity is the ability to hydrolyze the enzymatic hydrolyzate N-benzoyl-Ile-Glu-Gly-Arg-p-nitroanilide. A solution of the hydrolyzate was prepared in assay buffer at a concentration of 51 Mm (much less than Km = 1.3 mM). The final DMSO concentration was 4.3%. Purified activated Factor X was diluted to a concentration of 300 nM in assay buffer. The final reagent concentrations were: (FXa) = 10 nM, (N-benzoyl-Ile-Glu-Gly-Arg-p-nitroanilide hydrochloride) = 51 nM.

Plasmin: The standard for evaluating plasmin activity is the ability to hydrolyze N-p-toluenesulfonyl-Gly-Pro-Lys-p-nitroanilide. A solution of the hydrolyzate was prepared in assay buffer at a concentration of 37 mM (much less than Km = 243 mM). The final DMSO concentration was 4.3%. Purified human plasmin was diluted to a concentration of 240 nM in assay buffer. The final reagent concentrations were: (plasmin)=8nM, (N-p-toluenesulfonyl-Gly-Pro-Lys-p-nitroanilide)=37mM.

The results show that the compounds of the present invention are potent inhibitors of thrombin. The inhibition rate of some samples is listed as follows:

Example 105: Inhibition of Platelet Aggregation in Rats

Drugs and preparations: the compound of the present invention, prepared as a suspension with 0.5% CMC for administration to animals; adenosine diphosphate (ADP) (Sigma).

Instruments: PK121R centrifuge, SPA-3 PPP platelet aggregation analyzer.

Test method: Male Wistar rats with a body weight of about 300 grams were given clopidogrel sulfate and the compound of the present invention by oral gavage, the doses were 10 mg/kg, and the administration volume was 10 mL/kg. Blood was collected from the aorta, anticoagulated with 3.8% sodium citrate, the ratio of whole blood to anticoagulant was 9:1, and centrifuged at 1000rpm for 7 minutes to prepare platelet-rich plasma (PPP). Adjust PRP with PPP to keep the platelet count at 2X106/mL. Add PRP to the test cup and incubate at 37°C for 10 minutes. PRP was adjusted to zero, PPP was adjusted to 100%, and ADP (final concentration: 5 μM) was used as the inducer. The percentage of platelet aggregation was measured with the SPA-3 PPP platelet aggregator according to the Borns turbidimetric method, and the t-test was used for statistical comparison.

Inhibition of platelet aggregation induced by ADP, collagen and thrombin in vitro

Platelet Isolation: Human blood was obtained from healthy volunteers on call. Blood was collected in a 1/6 volume ACD (2.5 g sodium citrate, 1.5 g citric acid and 2.5 g glucose in 100 mL _H2O ). Platelet-rich plasma was removed by centrifugation at 800xg for 15 minutes at room temperature, incubated at 37°C for 60 minutes in the presence of 1 mM acetylsalicylic acid, and centrifuged at 1000xg for 10 minutes at room temperature. The platelet pellet was washed with HEPES-buffered Tyrodes solution (137mM NaCl, 2.7mM KCl, 1mM MgCl ₂ , 3mM NaH ₂ PO ₄ , 5mM glucose, 10mM HEPES Ph7.4, 0.2% bovine serum albumin) at a density of 2X108 cells/mL. and 0.05U/mL apyrase) to resuspend.

Aggregation studies: ADP-induced platelet aggregation was determined by measuring 0.5 mL of stirred (900 rpm) aspirin-treated washed platelets in an aggregometer at 37 degrees. The instrument was baselined with 0.5 mL of Hepes-buffered Tyrode's buffer. Before the aggregation assay, the platelet suspension was supplemented with 2 mM CaCl ₂ and 1 mg/mL fibrinogen. Addition of indicated concentrations of ADP or other agonist induces platelet aggregation, and light transmission is continuously recorded for at least 8 minutes. When testing inhibitors of platelet aggregation, platelets are incubated in the indicated concentration of inhibitor for 3-6 minutes, then ADP or other agonist is added, and the response is continuously recorded for at least 8 minutes. The data were fitted to a four-parameter logarithmic equation, and the potency of agonists and inhibitors of platelet aggregation was calculated from the two aggregation rates and the maximum degree of aggregation determined for each.

The results show that the compound of the present invention can significantly inhibit platelet aggregation induced by ADP, collagen or thrombin. Therefore, they can be used to prevent or treat coronary syndrome, myocardial infarction, myocardial ischemia and other cardiovascular and cerebrovascular diseases caused by platelet aggregation. Part of the sample inhibition _IC50 list is as follows:

Example 106: Effect on Platelet Adhesion (Collagen Adhesion Method)

25 rabbits were randomly divided into 5 groups, including blank control group, 3 dosage groups of the compound of the present invention and ticlopidine hydrochloride 104 mg/kg group. The test animals were administered ig at the above dose once a day for 3 consecutive days, and 1.5 hours after the last administration, blood was routinely collected from the ear vein to prepare PRP, PPP and acid-soluble collagen, and the siliconized turbidimetry was measured with a platelet aggregometer. The number of platelets before adding collagen into the tube (the final concentration of collagen is 0.05mg/mL) and the number of platelets (adjusting PRP with PPP to make the number of platelets 100,000-500,000/μL), and the platelet adhesion rate as the observation index, each administration group and the control group were compared. Comparison between groups, t test.

The test results show that the compound of the present invention has obvious inhibitory effect on the platelet adhesion of rabbits in vitro within the selected dosage range. The inhibition rate list of some samples is as follows:

compound Inhibition rate(%) 7-[2-(1-pyrrolidinyl)ethoxy]-4-chromone 59.2 7-[4-(1-pyrrolidinyl)butoxy]-4-chromone 38.4 7-[3-(4-Methylphenylthio)propoxy]-4-chromone 47.2 6-[4-(1-pyrrolidinyl)butoxy]-4-chromone 48.4 5-[2-(1-piperidinyl)ethoxy]-4-chromone 53.7 5-[4-(1-piperidinyl)butoxy]-4-chromone 49.2 7-[4-(1-pyrrolidinyl)butoxy]-4-chromanone 81.5 7-(2-Phenylthioethoxy)-4-chromanone 74.7 7-[4-(4-Methylphenylthio)butoxy]-4-chromanone 53.1 6-[3-(4-Morpholinyl)propoxy]-4-chromanone 47.5 5-[2-(Diethylamino)ethoxy]-4-chromanone 72.2 5-(3-Phenylthiopropoxy)-4-chromanone 52.3 7-[2-(Diethylamino)ethoxy]chroman 54.4 7-[(4-Morpholinyl)formylpropoxychroman 26.6 6-[2-(1-piperidinyl)ethoxy]chroman 37.7 6-(1-piperidinyl)formylethoxychroman 48.5 5-[4-(4-Morpholinyl)butoxy]chroman 70.1 5-(Dimethylamino)formylpropoxychroman 25.8

Example 107: Application in thrombotic diseases

The antithrombotic effect experiment of the compound of the present invention proves that it has a good effect in the prevention and treatment of thrombotic diseases. Compared with heparin, the applied dose is smaller and the effect is more obvious.

(1) Inhibit venous thrombosis

Ligation of the inferior vena cava in rats induces venous thrombosis. Intravenous injection was administered 2 hours after the model was established. The negative control group was given physiological saline, the positive control group was given 200-400 μg/kg of heparin, and the treatment group was given 10-200 μg/kg of the compound of the present invention. Four hours after administration, the thrombus was taken out and weighed. The results show that the compound of the present invention can obviously inhibit the formation of venous thrombus, and the required dose is lower than that of heparin.

name Weight (mg) Percentage reduction (%) model group 17.9 the heparin 15.3 14.5 7-[2-(1-pyrrolidinyl)ethoxy]-4-chromone 10.8 39.7 6-[3-(4-Morpholinyl)propoxy]-4-chromanone 9.6 46.4 6-(1-piperidinyl)formylethoxychroman 7.6 57.5

(2) Inhibit arterial thrombosis

Injure rabbit femoral artery endothelial cells with a balloon catheter, and ligate the blood vessels at the injured site to block the local blood flow and induce the formation of arterial thrombus. The negative control group was given physiological saline, the positive control group was given heparin 200-1000 μg/kg, and the treatment group was given 10-200 μg/kg of the compound of the present invention. Experimental results show that the compound of the present invention can reduce the rate of arterial thrombus formation in a dose-related manner.

name Weight (mg) Percentage reduction (%) model group 17.8 the heparin 12.6 29.2 5-[2-(1-piperidinyl)ethoxy]-4-chromone 7.8 56.2 7-(2-Phenylthioethoxy)-4-chromanone 7.5 57.9 7-[2-(Diethylamino)ethoxy]chroman 10.4 41.6

(3) Prevention and treatment of disseminated intravascular coagulation (DIC)

DIC formation was induced by injecting endotoxin into rabbits. Intravenous injection of the compound of the present invention can reduce platelet count, compared with the control group (physiological saline), there is a significant statistical difference.

name Percentage reduction (%) 5-[2-(1-piperidinyl)ethoxy]-4-chromone 56.7 7-[2-(1-pyrrolidinyl)ethoxy]-4-chromone 17.4 6-[3-(4-Morpholinyl)propoxy]-4-chromanone 46.8 7-[2-(Diethylamino)ethoxy]chroman 61.9 6-(1-piperidinyl)formylethoxychroman 39.2 control group 13.4

(4) Prevention and treatment of re-embolization after coronary angioplasty (PTCA)

Injury to the endothelial cells of the left anterior descending coronary artery in dogs induces obstructive coronary thrombosis. Using the compound of the present invention as an additional drug for streptokinase can promote coronary artery recanalization, inhibit re-embolization, and reduce the weight of residual thrombus in a dose-dependent manner. Compared with heparin, it has a higher recanalization rate, a shorter time to recanalization, a longer duration of reperfusion, and a lighter residual thrombus.

name Weight (mg) Percentage reduction (%) model group 16.6 the heparin 13.7 17.4 7-[2-(1-pyrrolidinyl)ethoxy]-4-chromone (small dose) 12.9 22.3 7-[2-(1-pyrrolidinyl)ethoxy]-4-chromone (medium dose) 9.3 44.0 7-[2-(1-Pyrrolidinyl)ethoxy]-4-chromone (high dose) 7.8 59.0 6-[3-(4-morpholinyl)propoxy]-4-chromanone (small dose) 12.5 24.7 6-[3-(4-morpholinyl)propoxy]-4-chromanone (medium dose) 10.3 38.0 6-[3-(4-morpholinyl)propoxy]-4-chromanone (high dose) 8.1 51.2

(5) Mouse pulmonary thrombosis test

100 mice were randomly divided into 5 groups, including a blank control group, 3 dosage groups of the compound of the present invention and a ticlopidine hydrochloride 100 mg/kg group. Intragastric administration, once a day, for 3 consecutive days, 1.5 hours after the last administration, the mice were injected with a strong compound inducer of collagen and epinephrine 5.0mL/kg (collagen 2.5mg/kg+adrenaline 2mg/kg ), the injection speed was 0.1mL/5 seconds, and the death of the mice was observed within 3 minutes, with the number of dead animals in each group and the protection rate as observation indicators. The test results are tested by X2, showing that the compound of the present invention has obvious protective effect on collagen and epinephrine-induced pulmonary thrombosis in mice within the selected dosage range.

name number of deaths Protection rate (%) blank group 16 the Ticlopidine Hydrochloride 12 25.0 7-[2-(1-pyrrolidinyl)ethoxy]-4-chromone (small dose) 11 31.2 7-[2-(1-pyrrolidinyl)ethoxy]-4-chromone (medium dose) 9 43.7 7-[2-(1-Pyrrolidinyl)ethoxy]-4-chromone (high dose) 7 56.3

Claims (6)

1. chroman compound, is selected from:

7-(dimethylin) formyl methoxyl group chroman;

7-(diethylin) formyl methoxyl group chroman;

7-(1-pyrrolidyl) formyl methoxyl group chroman;

7-(piperidino) formyl methoxyl group chroman;

7-(4-morpholinyl) formyl methoxyl group chroman;

6-(dimethylin) formyl methoxyl group chroman;

6-(diethylin) formyl methoxyl group chroman;

6-(1-pyrrolidyl) formyl methoxyl group chroman;

6-(piperidino) formyl methoxyl group chroman;

6-(4-morpholinyl) formyl methoxyl group chroman;

5-(dimethylin) formyl methoxyl group chroman;

5-(1-pyrrolidyl) formyl methoxyl group chroman;

5-(piperidino) formyl methoxyl group chroman;

5-(4-morpholinyl) formyl methoxyl group chroman.

2. a pharmaceutical composition, wherein containing the compound of claim 1 and pharmaceutical acceptable carrier or thinner.

3. compound according to claim 1 prevents and treats the application in the medicine of the disease relevant with platelet aggregation in preparation.

4. pharmaceutical composition according to claim 2 prevents and treats the application in the medicine of the disease relevant with platelet aggregation in preparation.

5. as the application of claim 3-4 as described in any one, it is characterized in that, the described disease relevant with platelet aggregation comprises the primary arterial thrombotic complication of arteriosclerosis disease, the thrombus complication that atheromatosis is intervened, the thrombus complication of operation or physical abuse, the platelet activation of mechanicalness induction, shunt occlusion, blood vessel injury and the insecondary thrombus of inflammation, phlebothrombosis, coronary artery thrombosis, atherosclerosis and arteriosclerosis, artery or the again obstruction of vein after molten scleroproein treatment, the platelet adhesion reaction relevant with extracorporeal circulation, the thrombus complication relevant with thromboembolism treatment, with the crown thrombus complication relevant with other angioplasty, or the thrombus complication relevant with coronary bypass-forming operation.

6. apply as claimed in claim 5, it is characterized in that, described arteriosclerotic primary arterial thrombotic complication refers to embolic stroke, peripheral vascular disease and does not have thromboclastic myocardial infarction; The thrombus complication of described atheromatosis intervention refers to that angioplasty, endarterectomy, support rotate, caused by crown and other vascular transplant; The thrombus complication of described operation or physical abuse refers to the tissue salvage of performing the operation or after accidental trauma, reconstructive procedure, and caused by " reductibility " operation; The platelet activation of described mechanicalness induction refers to and causes the cardiopulmonary bypass of microthrombus and blood product depository to cause; Described shunt occlusion is because of caused by Rend dialysis and plasma exchange; Described blood vessel injury and the insecondary thrombus of inflammation are because of caused by vasculitis, arteritis, glomerulonephritis and organ-graft refection; Described venous thrombosis is because of deep venous thrombosis, veno-occlusive disease, caused by thrombocytosis, polycyth(a)emia and migraine; Described coronary artery thrombosis is because of caused by unstable angina pectoris, coronary angioplasty and Acute Myocardial Infarction; Described atherosclerosis and arteriosclerosis refer to atherosclerosis, transient ischemia outbreak and apoplexy, peripheral vascular disease, arterial thrombus, embolism and angioplasty, carotid endarterectomy and vascular transplantation anastomosis after restenosis or close suddenly.