CN102920792B - Processed product of polygala tenuifolia with mangnolia officinalis and preparation method of same - Google Patents

Abstract

The invention belongs to the field of medicine, and in particular relates to a processed product of Magnolia officinalis Radix Polygalae and a preparation method thereof. The technical problem solved by the present invention is the processed product of Magnolia officinalis Polygala, which is used to alleviate the gastrointestinal side effects of Polygala, and retain the effect of Polygala "calming the mind, calming the nerves, eliminating phlegm and resuscitation". The processed product of Magnolia officinalis Radix Polygalae of the present invention is prepared by the following method: A, Magnolia officinalis and Polygala are according to crude drug ratio: 1-3 parts of Magnolia officinalis, 1 part of Polygala; 2 parts of Magnolia officinalis, 1 part of Polygala; B, preparation Magnolia officinalis juice: take Magnolia officinalis medicinal materials, add water to decoct, and filter to obtain Magnolia officinalis juice; C. Sunburn Polygala with Magnolia Juice: Soak Polygala with Magnolia Juice obtained in step B until Polygala absorbs Magnolia Juice, stir-fry and roast at 60-180°C for 6-18 minutes, after the juice is collected, dry to obtain processed Magnolia Polygala. The processed product of Magnolia Radix Polygalae of the present invention can effectively reduce the gastrointestinal motility inhibitory side effect of Radix Polygalae, and can retain the effects of antitussive, expectorant, and calming drugs, thereby achieving the purpose of reducing side effects and saving efficacy, and has clinical application value.

Description

Magnolia officinalis processed product and preparation method thereof

technical field

The invention belongs to the field of medicine, and in particular relates to a processed product of Magnolia officinalis Radix Polygalae and a preparation method thereof.

Background technique

Polygala is a traditional traditional Chinese medicine for calming the nerves and calming the nerves. It is mainly used for the treatment of restlessness, palpitations, insomnia, and forgetfulness. Modern pharmacological studies have shown that Polygala extract has sedative and anticonvulsant effects on the central nervous system, and its root bark, whole root and wood heart are fed to mice, which has a sedative and tranquilizing effect. However, raw polygala is highly toxic. Studies have shown that raw polygala can significantly inhibit gastrointestinal motility, hinder gastrointestinal digestion, and present obvious side effects such as flatulence, gastric mucosal damage, and inhibition of digestive enzymes. The low safety of clinical use restricts the safe and effective clinical application of polygala.

The inventor of the present invention has developed the Chinese patent application 200510021948.6, the involved honey-roasted polygala can reduce the damage of its gastric mucosa and partially relieve the inhibition of gastrointestinal motility. The inventor also used Magnolia officinalis and Polygala to improve its gastrointestinal side effects, such as "Comparison of chemical components and detoxification mechanism of Polygala and Magnolia before and after compatibility" [Chengdu University of Traditional Chinese Medicine, 2009 master's thesis], " Effects of polygala, Magnolia officinalis and their different ratios on isolated intestinal smooth muscle of rabbits" [Chinese medicine pharmacology and clinical 2011 No. 03], "Polygala and Magnolia officinalis with different decoction time and different ratios on gastrointestinal motility in mice "[Journal of Xi'an Jiaotong University (Medical Edition) 2009 No. 03], specifically investigated the effect of different compatibility of Magnolia officinalis and Polygala on the gastrointestinal The effect of side effects, the result is that decoction with a specific ratio can effectively relieve the gastrointestinal side effects of Polygala, and can retain the effect of Polygala "calming the mind, calming the nerves, eliminating phlegm and resuscitation".

On this basis, the inventors of the present invention provide a brand-new processed product of polygala for improving the gastrointestinal side effects of polygala.

Contents of the invention

The technical problem solved by the present invention is the processed product of Magnolia officinalis Polygala, which is used to alleviate the gastrointestinal side effects of Polygala, and retain the effect of Polygala "calming the mind, calming the nerves, eliminating phlegm and resuscitation".

Magnolia Radix Polygalae processed product of the present invention is prepared by following method:

A. Magnolia officinalis and polygala according to crude drug ratio: 1-3 parts of Magnolia officinalis, 1 part of polygala; preferably 2 parts of Magnolia officinalis, 1 part of polygala;

B, preparation of Magnolia officinalis juice: get Magnolia officinalis medicinal material, add water to decoct, filter to obtain Magnolia officinalis juice;

C． Sunburn Polygala with Magnolia Juice: Soak Polygala with Magnolia Juice obtained in step B until Polygala absorbs Magnolia Juice, stir-fry and roast at 60-180°C for 6-18 minutes, after the juice is collected, dry to obtain processed Magnolia Polygala.

The following is a further preferred process of the preparation method of Magnolia officinalis Radix Polygalae processed product of the present invention:

Further, the amount of water added to Magnolia officinalis in step B is 8-12 times the amount.

Further, in step B, the time for soaking Magnolia officinalis in water before decocting is 15-45min, preferably 30min.

Further, the magnolia bark decocting time in step B is 5-15min, preferably 10min.

Further, in order to reduce the time for soaking Polygala juice in step C, the filtered Magnolia juice can be concentrated to 0.2-0.8 amount of Polygala in step B, that is, based on 50 g of Polygala crude drug, step B will filter Magnolia bark The juice is concentrated into 10-40ml for easy absorption.

Further, the frying temperature in step C is preferably 120°C.

Further, the frying time in step C is preferably 12 minutes.

The processed product of Magnolia Radix Polygalae of the present invention can effectively reduce the gastrointestinal motility inhibitory side effect of Radix Polygalae, and can retain the effects of antitussive, expectorant, and calming drugs, thereby achieving the purpose of reducing side effects and saving efficacy, and has clinical application value.

Description of drawings

Figure 1 is the chromatogram of the mixed reference substance.

Figure 2 The chromatogram of the negative control of Polygala 20 minutes medicinal solution.

Figure 3 Honokiol reference substance mass and peak area standard curve.

Figure 4 Magnolol reference substance quality and peak area standard curve.

The chromatogram of the phenolic component of Fig. 5 Magnolia officinalis 10 minutes medical solution test sample.

The chromatogram of the phenolic component of Fig. 6 processed product 5 test samples.

Fig. 7 Chromatogram of polygala saponin reference substance.

Figure 8 Magnolia officinalis 10-minute medicinal liquid negative control chromatogram.

Fig. 9 standard curve of quality and peak area of Polygala saponin reference substance.

Fig. 10 Polygala saponin chromatogram of the 20-minute medicinal liquid test sample of Polygala.

Fig. 11 The chromatogram of polygala saponin of processed product 5 test sample.

Detailed ways

1. Experimental research

1 Experimental materials

1.1 Experimental animals

Kunming mice, 18-22 g, were provided by the Experimental Animal Center of Chengdu University of Traditional Chinese Medicine, certificate number: scxk (Sichuan) 2009-11.

1.2 Test drugs, main reagents, drugs and instruments

1.2.1 Test drugs

Polygala: purchased from the Hehuachi Traditional Chinese Medicine Market in Chengdu, identified as the root of Polygala tenuifolia willd by Professor Lu Xianming of Chengdu University of Traditional Chinese Medicine, and belongs to the species recorded in the 2010 edition of "Chinese Pharmacopoeia".

Magnolia Officinalis: Bought from the Hehuachi Traditional Chinese Medicine Market in Chengdu, identified as Magnolia officinalis Rehd.et Wils. and Magnolia officinalis Rehd.et Wils.var.biloba Rehd.et Wils. by Professor Lu Xianming of Chengdu University of Traditional Chinese Medicine Root bark is a species recorded in the 2010 edition of "Chinese Pharmacopoeia".

1.2.2 Main reagents and drugs

Phenol red, Chengdu Kelong Chemical Reagent Factory, production batch number: 0100901

Anding, Changzhou Siyao Pharmaceutical Co., Ltd., production batch number: 20111108

Ammonium chloride, Chengdu Kelong Chemical Reagent Factory, production batch number: 110308

Cisapride, Zhejiang Jingxin Pharmaceutical Co., Ltd., production batch number: 1012161

Compound Licorice Tablets, Qinghai Pharmaceutical Factory Co., Ltd., production batch number: 20111246

Sodium pentobarbital, Beijing Chemical Reagent Company, production batch number: Q/H82-F158-2002

Methanol, analytically pure, Chengdu Kelong Chemical Reagent Factory, production batch number: 20120327

Sodium hydroxide, analytically pure, Chengdu Kelong Chemical Reagent Factory, production batch number: 20110921

Phosphoric acid, analytically pure, Shanghai Chemical Reagent General Factory, production batch number: 20080625

Ammonia water, analytically pure, Chengdu Kelong Chemical Reagent Factory, production batch number: 20110418

Butanol, analytically pure, Chengdu Kelong Chemical Reagent Factory, production batch number: 20110826

Purified water, Robust (Guangdong) Drinking Water Co., Ltd. Chengdu Branch

Chromatographic methanol, chromatographically pure, Fisher company, production batch number: 111270

Magnolol reference substance, Chengdu Master Biotechnology Co., Ltd., purity ≥ 98%, production batch number: 11050601

Honokiol reference substance, Chengdu Master Biotechnology Co., Ltd., purity ≥ 98%, production batch number: 11050602

Polygala saponin reference substance, Chengdu Master Biotechnology Co., Ltd., purity ≥ 98%, production batch number: 11042801

1.2.3 Experimental Instruments

CP124S electronic analytical balance (SARTORIUS)

VARIOSKAN FLASH 2.4.3 full-wavelength multi-function reader (Thermo Company)

Shimadzu LC-20AT high performance liquid chromatography (SHIAZDU company)

Micro sample injector (25ul, Hamilton company)

GL-20G-Ⅱ refrigerated centrifuge (Shanghai Anting Scientific Instrument Factory)

ZZ-6 mouse autonomous activity tester (Chengdu Taimeng Technology Co., Ltd.)

Boston Green ODS C18 column (4.6×250mm, 5μm, )

1.3 Experiment site and animal certificate number

Chengdu University of Traditional Chinese Medicine State Administration of Traditional Chinese Medicine Class III Pharmacology Laboratory of Traditional Chinese Medicine, certificate registration number: TCM-2009-135. Laboratory animal certificate: SCXK (Sichuan) 2009-11.

2 Experimental statistical software and methods

One-way analysis of variance and chi-square test were carried out by SPSS13.0 statistical software.

3 Experimental methods and results

3.1 Conditional design of Magnolia officinalis Juice Sunburn Polygala medicinal liquid based on orthogonal experiment

Using 7 factors and 3 levels of orthogonal test table, select A (proportion of Polygala officinalis Magnolia officinalis), B (time for decocting Magnolia officinalis), C (concentrated volume of Magnolia officinalis juice), D (stir frying temperature), E (stir frying time) , F (Polygalus decocting time) 6 factors, draw up 18 groups of different processes for concocting liquid medicine.

The process of making Magnolia officinalis Zhigala medicinal liquid is as follows: Magnolia officinalis medicinal material A (n*50) g→soaked for 30 minutes→boiled with strong fire, decocted at slow fire for Bmin→concentrated to Cml under reduced pressure to make Magnolia officinalis juice→make Magnolia officinalis Add the medicinal juice to 50g Polygala raw medicinal materials, moisten it for 3 hours→stir-fry Polygala Emin at D degree Celsius→spread out the fried Polygala and dry it→soak Polygala processed product in 10 times of water for 30min→boil with strong fire, decoct with slow fire Fmin→concentrate under reduced pressure to 50ml→constant volume to 100ml→concoct the medicinal solution containing 0.5g/ml raw medicinal materials. The factors and levels are shown in Table 1 below, and the experimental arrangement is shown in Table 2.

Table 16 Factor 3 level table

Table 2 Experiment Arrangement

3.2 Based on the gastric residual rate and intestinal propulsion test, the processed products for alleviating gastrointestinal motility disorders are selected

3.2.1 Preparation of liquid medicine

Polygala medicinal liquid: Soak 50g Polygala raw medicinal materials in 10 times of water for 30 minutes→boil with strong fire, decoct with slow fire for 60min→concentrate under reduced pressure to 50ml→concentrate to 100ml→process the medicinal solution containing 0.5g/ml of raw medicinal materials.

Magnolia officinalis medicinal liquid: Soak 50g of magnolia officinalis in 10 times of water for 30 minutes→boil with strong fire, decoct in slow fire for 15min→concentrate under reduced pressure to 50ml→concentrate to 100ml→concoct the liquid containing 0.5g/ml of raw medicinal materials.

Compatible medicinal liquid: Soak 50g Polygala officinalis in 10 times of water for 30min→boil with strong fire, decoct with slow fire for 45min→add 100g of raw Magnolia officinalis medicinal material soaked in 10 times of water for 30min→boil with strong fire, decoct with slow fire for 15min→concentrate to 50ml under reduced pressure→ Set the volume to 100ml → concoct the medicinal solution containing 1.5g/ml of crude drug.

Magnolia officinalis Zhizhi medicinal liquid: preparation as in 3.1 to prepare groups of processed products 1-18.

3.2.2 Experimental method of gastric emptying and small intestine propulsion

Take 300 healthy Kunming mice, half male and half male, weighing 18-22 g. According to sex and body weight, they were randomly divided into blank group, Polygala group, Magnolia officinalis group, compatibility group, processed 1 group, processed 2 group, 3, 4, 5, 6... 18 groups, 10 animals in each group (details for grouping and dosage) See Table 3-7). Before the experiment, fasting without food and water for 24 hours, each group was given the corresponding medicinal solution by intragastric administration of 0.2ml/10g, and the blank group was given the same volume of normal saline. After 40 minutes of administration, the mice were given nutritional semi-solid paste (10g CMC-Na, 250ml normal saline, 16g milk powder, 8g sugar, 8g starch, 2g activated carbon powder, stirred evenly, and prepared into 300ml, about 300g black semi-solid paste) Solid paste. Refrigerate in the refrigerator, return to room temperature when used.) 0.4ml/10g, 20min later, kill by neck dislocation.

The abdominal cavity of the mouse was dissected, the gastric cardia and pylorus were ligated, the stomach was taken out, dried with filter paper and weighed, then the gastric body was cut along the greater curvature of the stomach, the contents of the stomach were washed away and dried, and the net weight was calculated to calculate the gastric residual rate (% ).

Stomach residual rate (%)=[(whole stomach weight - net stomach weight)/charcoal paste weight]×100%

The abdominal cavity of the mouse was dissected, and the pylorus from the stomach to the ileocecal was cut. The entire small intestine was taken out with tweezers without traction, laid straight on a board with a wet surface, the distance from the pylorus to the front of the charcoal paste and the total length from the pylorus to the ileocecal were measured, and the propulsion rate of the small intestine was calculated.

Small intestine propulsion rate (%) = (moving distance of charcoal paste/full length of pluvialis) × 100%

The obtained results were statistically analyzed by one-way analysis of variance.

3.2.3 Experimental results

The results are shown in Table 3~Table 7.

Table 3 Effects of Sunburning Polygala Magnolia Juice on Gastric Residue Rate and Intestinal Propulsion Rate in Mice (1)

Note: Compared with blank group, *P<0.05; compared with Polygala group, △P<0.05

Table 4 The effect of stewed Polygala magnolia juice on gastric residual rate and intestinal propulsion rate in mice (2)

Note: Compared with Magnolia officinalis group, *P<0.05, **P<0.01; compared with Polygala group, △P<0.05, △△P<0.01

Table 5 The effect of stewed polygala with Magnolia officinalis juice on gastric residual rate and intestinal propulsion rate in mice (3)

Note: Compared with Magnolia officinalis group, *P<0.05, **P<0.01; compared with Polygala group, △P<0.05, △△P<0.01

Table 6 The effect of stewed polygala with Magnolia officinalis juice on gastric residual rate and intestinal propulsion rate in mice (4)

Note: Compared with Magnolia officinalis group, **P<0.01; compared with Polygala group, △P<0.05, △△P<0.01

Table 7 The effect of stewed polygala with Magnolia officinalis juice on gastric residual rate and intestinal propulsion rate in mice (5)

Note: Compared with Magnolia officinalis group, *P<0.05; compared with Polygala group, △P<0.05

The results showed that the processed product group 5 and the processed product group 12 could significantly alleviate the inhibition of gastrointestinal motility caused by polygala. Therefore, these two processed products of Magnolia officinalis Juice and Radix Polygala were selected for repeated experiments and related pharmacodynamic investigations.

3.3 Repeated experiments on the influence of optimized processed products on gastrointestinal motility

3.3.1.1 Preparation of liquid medicine

Polygala medicinal liquid for 20 minutes: Soak 50g Polygala raw medicinal materials in 10 times of water for 30 minutes→boil with strong fire, decoct with slow fire for 20min→concentrate under reduced pressure to 50ml→concentrate to 100ml→process the medicinal solution containing 0.5g/ml of raw medicinal materials.

Magnolia officinalis medicinal liquid for 10 minutes: Soak 50g of magnolia bark raw medicinal materials in 10 times of water for 30 minutes → boil with strong fire, decoct with slow fire for 10 minutes → concentrate under reduced pressure to 50ml → dilute to 100ml → concoct the medicinal liquid containing 0.5g/ml of raw medicinal materials.

Magnolia Juice Radix Polygala Medicinal Solution 5: Magnolia officinalis medicinal material 100g→soak for 30min→boil with strong fire, decoct with slow fire for 10min→concentrate to 25ml under reduced pressure to make magnolia medicinal juice→add magnolia medicinal juice to 50g Polygala medicinal material, Moisturize for 3 hours→stir-fry Polygala at 120 degrees Celsius for 12 minutes→spread out the fried Polygala and dry it→soak the processed Polygala in 10 times of water for 30min→boil with strong fire and simmer for 20min→concentrate under reduced pressure to 50ml→concentrate to 100ml.

Magnolia Juice Radix Polygalis Medicinal Solution 12: 50g of Magnolia officinalis → soak for 30 minutes → boil with strong fire, decoct with slow fire for 15 minutes → reduce pressure and concentrate to 25ml to make Magnolia officinalis juice → add Magnolia officinalis to 50g of Polygala, Moisturize for 3 hours→stir-fry Polygala at 90 degrees Celsius for 6 minutes→spread out the fried Polygala and dry it→soak the processed Polygala in 10 times water for 30min→boil with strong fire, decoct for 20min on low heat→concentrate under reduced pressure to 50ml→concentrate to 100ml.

3.3.1.2 Experimental method

Take 84 healthy Kunming mice, half male and half male, weighing 18-22 g. The mice were randomly divided into blank group, cisapride group, Polygala 20-minute group, Magnolia officinalis 10-minute group, processed 5 groups, and processed 12 groups according to sex and body weight, with 14 mice in each group. According to 3.2.2 gastric residual, intestinal propulsion test method to carry out the experiment.

3.3.1.3 The experimental results are shown in Table 8.

Table 8 Repeated experiments on the effect of magnolia bark juice on Polygalaceae polygala on gastric residue and intestinal propulsion in mice

Note: Compared with blank group, **P<0.01, *P<0.05; compared with Polygala group, △△P<0.01, △P<0.05

The results showed that both the processed product 5 group and the processed product 12 group could significantly reduce the gastric residual rate and increase the small intestinal propulsion rate (P<0.05), but the gastric residual rate of the processed product 12 group was significantly higher than that of the blank group (P<0.05 ), suggesting that the processed product still affects gastric motility; while the processed product 5 has no significant difference with the blank group (P>0.05), indicating that the processed 5 group is closer to normal mice. In addition, in the pentobarbital sodium synergistic test, the processed product 12 did not show the effect of calming the nerves, so only the processed product 5 was selected for the subsequent pharmacodynamic experiments.

3.3.2.1 Preparation of liquid medicine

①Polygalae medicinal liquid: Soak 50g Polygala raw medicinal materials in 10 times of water for 30 minutes→boil with strong fire, decoct with slow fire for 20min→concentrate under reduced pressure to 50ml→concentrate to 100ml→the medicinal solution contains 0.5g/ml of raw medicinal materials.

② Magnolia officinalis medicinal liquid: Soak 100g of magnolia officinalis in 10 times of water for 30 minutes→boil with strong fire, decoct in slow fire for 10min→concentrate under reduced pressure to 50ml→concentrate to 100ml→the liquid contains 1.0g/ml of raw medicinal materials.

③High dosage of Magnolia officinalis Zhizhi medicinal liquid: Magnolia officinalis medicinal material 100g→soak for 30min→boil with strong fire, decoct on slow fire for 10min→concentrate to 25ml under reduced pressure to make Magnolia officinalis medicinal juice→add Magnolia bark medicinal juice to 50g Polygala medicinal material Medium, moisten for 3 hours→stir-fry polygala at 120 degrees Celsius for 12 minutes→spread out the fried polygala and dry it→soak the processed polygala in 10 times of water for 30 minutes→boil with strong fire and simmer for 20 minutes→concentrate under reduced pressure to 50ml→concentrate to 100ml→ The medicinal liquid contains 1.5g/ml of raw medicinal materials.

④Compatible medicinal liquid: Soak 50g Polygala officinalis in 10 times of water for 30min→boil with strong fire, decoct with slow fire for 10min→add Magnolia officinalis and soak 100g of Magnolia officinalis in 10 times of water for 30min→boil with strong fire, decoct with slow fire for 10min→concentrate under reduced pressure to 50ml → dilute to 100ml → the liquid contains 1.5g/ml raw medicinal materials.

3.3.2.2 Experimental method

Take 84 healthy Kunming mice, half male and half male, weighing 18-22 g. They were randomly divided into blank group, cisapride group, Polygala group, Magnolia officinalis group, compatibility group and processed high-dose group according to sex and body weight, with 14 rats in each group. Before the experiment, fasting without food and water for 24 hours, each group was given the corresponding medicinal solution by intragastric administration of 0.2ml/10g, and the blank group was given the same volume of normal saline. After 40 minutes of administration, the mice were given nutritional semi-solid paste (10g CMC-Na, 250ml normal saline, 16g milk powder, 8g sugar, 8g starch, 2g activated carbon powder, stirred evenly, and prepared into 300ml, about 300g black semi-solid paste) Solid paste. Refrigerate in the refrigerator, return to room temperature when used.) 0.4ml/10g, 20min later, kill by neck dislocation.

The abdominal cavity of the mouse was dissected, the gastric cardia and pylorus were ligated, the stomach was taken out, dried with filter paper and weighed, then the gastric body was cut along the greater curvature of the stomach, the contents of the stomach were washed away and dried, and the net weight was calculated to calculate the gastric residual rate (% ).

Stomach residual rate (%)=[(whole stomach weight - net stomach weight)/charcoal paste weight]×100%

The abdominal cavity of the mouse was dissected, and the pylorus from the stomach to the ileocecal was cut. The entire small intestine was taken out with tweezers without traction, laid straight on a board with a wet surface, the distance from the pylorus to the front of the charcoal paste and the total length from the pylorus to the ileocecal were measured, and the propulsion rate of the small intestine was calculated.

Small intestine propulsion rate (%) = (moving distance of charcoal paste/full length of pluvialis) × 100%

3.3.2.3 The experimental results are shown in Table 9.

Table 9 Experimental results of Sunburning Polygala Magnolia Juice on Stomach Residue and Intestinal Propulsion in Mice

Compared with Polygala group, △△P<0.01, △P<0.05

The results showed that compared with Polygala group, blank group, cisapride group, Magnolia officinalis group, compatibility group and processed high-dose group could significantly reduce the gastric residual rate of mice (△△P<0.01, △P<0.05); group, cisapride group, magnolia bark group and processed high-dose group can significantly increase the intestinal propulsion rate of mice (△△P<0.01, △P<0.05). It shows that the processed high-dose group can significantly alleviate the gastrointestinal motility disorder caused by polygala, and the effect is better than that of the parallel-prepared compatibility group.

3.4 Study on pharmacodynamics of processed pentobarbital sodium in synergy with hypnosis and antitussive

①Polygalae medicinal liquid: Soak 50g Polygala raw medicinal materials in 10 times of water for 30 minutes→boil with strong fire, decoct with slow fire for 20min→concentrate under reduced pressure to 50mL→concentrate to 100mL, concentrate to a medicinal solution containing raw medicinal materials at a concentration of 0.5g/mL.

②Magnolia officinalis medicinal liquid: Soak 100g of magnolia officinalis in 10 times of water for 30 minutes→boil with strong fire, decoct in slow fire for 10min→concentrate under reduced pressure to 50mL→concentrate to 100mL, concentrate to a medicinal solution containing raw medicinal materials at a concentration of 1.0g/mL .

③ High-dose medicinal liquid for processed products: Magnolia officinalis medicinal material 100g→soak for 30min→boil with strong fire, decoct with slow fire for 10min→concentrate to 25ml under reduced pressure to make magnolia officinalis medicinal juice→add magnolia Wet for 3 hours→Stir-fry Polygala at 120 degrees Celsius for 12min→Spread out the fried Polygala and dry it→Soak the processed Polygala in 10 times of water for 30min→Boil with strong fire, decoct with slow fire for 20min→Concentrate under reduced pressure to 50ml→Concentrate to 100ml, concentrate to contain The medicinal liquid with the concentration of raw medicinal materials is 1.5g/mL.

④Medium-dose medicinal liquid for processed products: Take 50ml of high-dose medicinal liquid and dilute to 100ml.

⑤ Low-dose medicinal liquid for processed products: Take 50ml of medium-dose medicinal liquid and dilute to 100ml.

3.4.1 Experimental method and results of pentobarbital sodium synergistically hypnotizing mice

98 healthy Kunming mice were randomly divided into 7 groups according to sex and body weight: blank group, diazepam group, Polygala group, Magnolia officinalis group, processed high-dose group, processed medium-dose group and processed low-dose group , 14 in each group. Before the experiment, fasting without food and water for 9 hours, 0.2mL/10g orally administered the corresponding medicinal solution, and the blank group was given the same volume of normal saline. After 30 minutes of administration, freshly prepared 30 mg/kg pentobarbital sodium solution (150 mg to 100 mL) was intraperitoneally injected. The righting reflex of the mice disappeared for more than 1 min as the standard for falling asleep. The percentage of mice falling asleep within 15 min in each group was counted. The results are shown in Table 10.

Table 10 The effect of different doses of Magnolia officinalis Juice Sunburn Polygala on the hypnotic effect of pentobarbital sodium in synergistic mice

Compared with blank group, *P<0.05, **P<0.01

The results showed that, compared with the blank group, the diazepam group, the processed product high-dose group, and the processed product medium-dose group could significantly enhance the hypnotic effect of pentobarbital sodium on mice (P<0.05).

3.4.2 Experimental method and results of antitussive effect on mice cough induced by ammonia water

98 healthy Kunming mice were randomly divided into 5 groups according to body weight: blank group, compound licorice tablet group, Polygala group, Magnolia officinalis group, processed high-dose group, processed medium-dose group and processed low-dose group. 14 in each group. Before the experiment, fasting without food and water for 9 hours, the corresponding medicinal solution was given by intragastric administration at 20 mL/kg, and the blank group was given the same volume of normal saline. One hour after the administration, the mice were placed in a 250-mL jar with a cotton ball in it, and 0.1 mL of 25% to 28% ammonia water was sucked into the cotton ball with a pipette gun each time. Coughing is manifested by the contraction of the abdominal muscles of the mouse and the opening of the mouth at the same time, sometimes with a coughing sound. Observe and record the initial coughing time and coughing times of the mice within 3 min. The results are shown in Table 11.

Table 11 The antitussive effect of different doses of Magnolia officinalis Juice Sunburn Polygala on ammonia water-induced cough in mice

The results showed that, compared with the blank group, each administration group could significantly reduce the cough frequency of mice induced by ammonia water (P<0.01); Cough latency of mice (P<0.01), Polygala group and Magnolia officinalis group can significantly prolong the latency of cough induced by ammonia water (P<0.05).

3.5 Effects of different doses on expectorant effect and autonomous activity in mice

3.5.1 Effect of different doses on expectorant effect in mice

(1) Preparation of 1% phenol red

Preparation of 1% phenol red: Dissolve 1 g of phenol red in 11.28 ml of NaOH saline solution with a concentration of 0.04 g/100 ml, grind the phenol red until there are no fine particles, filter through the funnel, wash the funnel repeatedly with normal saline, and put Dilute it to a 100ml volumetric flask, and get ready.

(2) Experimental methods and results

112 healthy Kunming mice were fasted for 9 hours, and randomly divided into blank group, Magnolia officinalis interference control group, ammonium chloride group, Polygala group, Magnolia officinalis group, processed high-dose group, and processed medium group according to body weight. Dose group and processed product low-dose group, a total of 8 groups, 14 rats in each group. Mice were given the corresponding drug solution by intragastric administration at 20 mL/kg, and the blank group was given the same volume of normal saline. After 30 minutes of administration, except the magnolia bark interference control group was injected with 10 mL/kg of normal saline intraperitoneally, the other 7 groups were injected with 1% phenol red at 10 mL/kg of body weight, and the mice were sacrificed 30 minutes later, the tissues around the trachea were peeled off, and cut out A section of the trachea from the thyroid cartilage to the branch of the trachea was put into a test tube containing 2 mL of normal saline, and then 0.1 mL of sodium hydroxide solution (1mol/L) was added, shaken for 10 min, centrifuged at 3000 rpm for 15 min, and the supernatant was added to 96 Orifice plates were measured with a microplate reader at a wavelength of 546 nm to measure the absorbance (OD) value.

In order to exclude the interference of magnolol on the phenol red experiment, a magnolol interference control group was specially set up to exclude the interference of magnolol on the absorbance of specific wavelengths. The experimental procedure is as follows: intragastric administration of 20ml/kg of the corresponding magnolia bark liquid to mice, 30min after administration, intraperitoneal injection of normal saline at 0.1ml/10g of body weight, 30min to kill the mice, and the procedure is the same as above for determination. The obtained results were statistically analyzed by one-way analysis of variance. The results are shown in Table 12.

Table 12 Effects of different doses of Magnolia officinalis Juice Sunburned Polygala on the Excretion of Phenol Red in Mice

The results showed that the OD value of the Magnolia officinalis interference control group was not significantly different from that of the blank group (P>0.05), and its OD value was lower than that of the blank group. Compared with the blank group, both the ammonium chloride group and the high-dose processed product group could significantly increase the OD value of phenol red in the trachea (P<0.05, P<0.01), and the polygala group and Magnolia officinalis group had higher OD values than the blank group. trend, but there was no significant difference (P>0.05).

3.5.2 Effects of Different Doses on Autonomous Activities of Mice

Take 98 healthy Kunming mice. Adaptive feeding for 3 days, and fixed at 13:00 every day, the mice were placed in the mouse autonomous activity measuring instrument for 3 minutes, and the activity value of the mouse was recorded for 5 minutes, and the activity value was the sum of the number of activities and the number of standing. Mice that were too active or very inactive were eliminated, and those with an average activity value between 80 and 200 were selected as qualified mice. On the 4th day of the experiment, the activity value of the mice was measured and recorded again as the basic activity value before administration, and the mice were randomly divided into blank groups, diazepam group, polygala group, magnolia bark group, There were 7 groups including high-dose processed product group, medium-dose processed product group and low-dose processed product group, with 14 rats in each group. On the 2nd day after the grouping, which is the 1st day of the formal experiment, each drug group was given the corresponding drug solution by intragastric administration at 20 mL/kg, and the blank group was given the same volume of normal saline for 7 consecutive days. On the 1st, 3d, and 7th days of the formal experiment, the mice were administered for 30 minutes, and the number of activities and standing times of the mice were measured in the same way as the activity values of the mice after administration.

The activity inhibition rate was used to reflect the change of the autonomous activity of the mice.

Activity inhibition rate = [(activity value after administration - activity base value) / activity base value] × 100%

The obtained results were analyzed by Kruskal using sas8.0. The experimental results are shown in Table 13.

Table 13 The effect of different doses of Magnolia officinalis Juice Sunburn Polygala on the change of autonomic activity in mice

The results showed that there was no significant difference among the components before administration (P>0.05). After administration, compared with the blank group, the diazepam group and the processed high-dose group could significantly inhibit the spontaneous activity of the mice in 1d, 3d, and 7d (P<0.05, P<0.01), and the processed medium-dose group was at 1d, 3d and polygala group can significantly inhibit the autonomous activity of mice on 3d (P<0.05), presenting a sedative effect.

3.6 Determination of phenolic components in Magnolia bark and Magnolia officinalis Juice

3.6.1 Experimental materials and instruments

Magnolol and honokiol reference substances (purity greater than 98.0%, Chengdu Master Biotechnology Co., Ltd.), chromatographic methanol are chromatographically pure, methanol, purified water and other reagents are analytically pure.

Shimadzu LC-20AT high performance liquid chromatography (SHIAZDU company), UV detector, CP124S electronic analytical balance (SARTORIUS).

3.6.2 Chromatographic conditions and system adaptability test

Boston Green ODS C18 column (4.6×250mm, 5μm, ); the mobile phase methanol-water (78:22) was the mobile phase; the flow rate was 1ml·min ^-1 ; the column temperature was 30°C; the detection wavelength was 294nm. The degree of separation is greater than 1.5, and the number of theoretical plates is not less than 3800 based on the peak of magnolol.

3.6.3 Sample preparation

Preparation of Mixed Reference Substance Solution Take appropriate amount of magnolol reference substance and honokiol reference substance, weigh them accurately, add methanol and mix to make a solution containing 40ug of magnolol and 24ug of honokiol per 1ml. The resulting chromatogram is shown in Figure 1.

Preparation of the test solution Precisely measure Polygala 20-minute liquid, Magnolia officinalis 10-minute liquid, 5 processed products 2ml each, add methanol to a 10ml volumetric flask, and shake. Pass through a 0.45um microporous membrane to obtain it.

3.6.4 Negative interference test

Take 10ul of Polygala test sample under item 3.6.3, inject the sample according to the chromatographic conditions under item 3.6.2, detect and find no interference. The results showed that Polygala single herb liquid did not interfere with the determination of Magnolia officinalis liquid components, see Figure 2 for details.

3.6.5 Linear relationship inspection

Take respectively 2ul, 4ul, 6ul, 8ul, 10ul, 12ul, 18ul of magnolol and honokiol mixed reference substance under item 3.6.3 and the mixed control whose concentration is 48ug of honokiol and 80ug of magnolol per 1ml Product 10ul sample injection, obtain its mass and the relation of peak area, calculate regression equation and ^R2 value, the ^R2 value of honokiol and magnolol is 0.9994, shows that the linear relationship is good. The results are shown in Tables 14 and 15 and Figures 3 and 4 below.

The mass and peak area relationship of table 14 honokiol

serial number 1 2 3 4 5 6 7 8 M (ug) 0.048 0.096 0.144 0.192 0.240 0.288 0.432 0.480 A 42819.5 83818.1 126579.2 163625.3 201892.5 342478.6 353334.5 402303.3

The mass and peak area relation of table 15 magnolol

serial number 1 2 3 4 5 6 7 8 M (ug) 0.080 0.160 0.240 0.320 0.400 0.480 0.720 0.800 A 64260.4 127922.3 191732.4 252755.8 314245.9 375578.6 547515.8 625570.7

3.6.6 Precision test

Take 10ul of the mixed reference substance solution under item 3.6.3, inject 6 needles continuously according to the chromatographic conditions under item 3.6.2, obtain the peak area, and calculate the RSD value of the peak area. The results are shown in Table 16.

Table 16 Mixed reference substance precision test

The results showed that the RSD values were all less than 2%, so the precision of the instrument was good.

3.6.7 Stability test

Take 10ul of the mixed reference substance and magnolia bark test substance under item 3.6.3, and inject samples at 0, 2, 4, 8, 12, and 24 hours respectively according to the chromatographic conditions under item 3.6.2. Get the peak area, and calculate the RSD value of the peak area, the results are shown in Tables 17 and 18.

Table 17 Mixed reference substance stability test

Table 18 Magnolia officinalis test product stability test

It can be seen from the results that the RSDs are all less than 2%, and the mixed reference substance and the test solution are basically stable within 24 hours.

3.6.8 Repeatability test

Prepare 6 samples in parallel according to the preparation of the magnolia bark test product under item 3.6.3, take 10ul for each, and inject samples according to the chromatographic conditions under item 3.6.2. The peak area was obtained, and the RSD value of the peak area was calculated. The results are shown in Table 19.

Table 19 Magnolia bark test product repeatability test

The results showed that the RSD values were all less than 3%, indicating that the repeatability of the sample preparation method was good.

3.6.9 Sample recovery test

Parallel precision measure 2ml of Magnolia officinalis water decoction which is the same as the content determination of 6 parts, add magnolol and honokiol reference substance in appropriate amount, and prepare according to the preparation method of the test sample under 3.6.3. Take 10ul from each portion and inject according to the chromatographic conditions under 3.6.2. The recovery rate and RSD value were calculated, and the obtained results are shown in Tables 20 and 21 below.

Table 20 honokiol sample addition recovery test

Table 21 Magnolol sample addition recovery test

The results showed that the recoveries were in the range of 95%-105%, and the RSDs were all less than 2%. Prompt sample preparation method is feasible.

3.6.10 Determination of honokiol and magnolol content in Magnolia officinalis and Magnolia officinalis Zhizhi

According to the sample preparation method in 3.6.3, prepare 3 copies of Magnolia officinalis 10-minute medicinal solution and 5 test samples in parallel. According to the chromatographic conditions in 3.6.2, inject 10ul of sample, and inject 3 times in parallel for each sample. The average content and RSD value of honokiol and magnolol in the liquid medicine were determined. The obtained results are shown in Tables 22 and 23, and the chromatograms of the 10-minute medicinal solution of magnolol and the 5 phenolic components of the processed product are shown in Figures 5 and 6.

Table 22 honokiol content assay results

Table 23 Magnolol Content Determination Results

It can be seen from the results that the RSDs were all within 3%. The content of honokiol and magnolol in processed product 5 was slightly lower than that of Magnolia officinalis 10 minutes liquid.

3.7 Determination of Saponins in Polygala Polygalae and Magnolia officinalis Juice

3.7.1 Experimental materials and instruments

Polygala saponin (purity greater than 98.0%, Chengdu Master Biotechnology Co., Ltd.), chromatographic methanol is chromatographically pure, methanol, purified water, phosphoric acid and other reagents are analytically pure.

Shimadzu LC-20AT high performance liquid chromatography (SHIAZDU company), UV detector, CP124S electronic analytical balance (SARTORIUS).

3.7.2 Chromatographic conditions and system adaptability test

Boston Green ODS C 18 chromatographic column (4.6×250mm, 5μm, ); the mobile phase methanol-0.05% phosphoric acid solution (55:45) is the mobile phase; the flow rate is 1ml·min ^-1 ; the column temperature is 30°C; the detection wavelength is 210nm. The degree of separation is greater than 1.5, and the number of theoretical plates is not less than 3000 based on the peak of polygala saponin.

3.7.3 Sample preparation

Preparation of Polygala saponin Reference Substance Solution Take an appropriate amount of Polygala saponin reference substance, weigh it accurately, add methanol to make a solution containing 1 mg per 1 ml, and obtain it. The resulting chromatogram is shown in Figure 7.

Preparation of the test solution Take the supernatants of Magnolia officinalis 10 minutes medicinal liquid, polygala 20 minutes medicinal liquid, and processed product 5 after standing, after centrifuging at 3000rmp for 10min, accurately measure 5ml of each centrifugal medicinal liquid, add 11% 50ml of sodium hydroxide solution was prepared into 55ml of 10% sodium hydroxide solution, heated and refluxed for 2 hours, allowed to cool, adjusted to pH 4-5 with hydrochloric acid, extracted by shaking with n-butanol saturated with water 3 times, 50ml each time, Combine the n-butanol solution, recover the solvent to dryness, add an appropriate amount of methanol to dissolve the residue, transfer it to a 25ml measuring bottle, add methanol to the mark, and shake well. Pass through a 0.45um microporous membrane to obtain it.

3.7.4 Negative interference test

Take 10ul of the Polygala test sample under item 3.7.3, inject the sample according to the chromatographic conditions under item 3.7.2, detect and find no interference. The results show that the single medicinal liquid of Magnolia officinalis does not interfere with the determination of the components of Polygala medicinal liquid, see Figure 8 for details.

3.7.5 Linear relationship inspection

Take 2ul, 4ul, 6ul, 8ul, 10ul, 12ul, and 16ul samples of the reference substance of Polygala saponin under item 3.7.3 respectively, obtain the relationship between its mass and peak area, calculate the regression equation and R ² value, Polygala saponin The ^R2 value is 0.9996, indicating that the linear relationship is good, the results are shown in Table 24 and Figure 9 below.

Table 24 The relationship between the quality and peak area of polygala saponins

serial number 1 2 3 4 5 6 7 M (ug) 1 2 3 4 5 6 8 A 172783.4 341972.8 523809.3 698078.8 874684.4 1056384.0 1377819.3

3.7.6 Precision test

Take 10ul of Polygala saponin reference solution under item 3.7.3, inject 6 needles continuously according to the chromatographic conditions under item 3.7.2, obtain the peak area, and calculate the RSD value of the peak area. The results are shown in Table 25.

Table 25 Precision Test of Polygala saponin

serial number 1 2 3 4 5 6 RSD (%) Polygala saponin 874684.4 888647.7 869054.6 887554.2 899902.0 885456.1 1.24

The results showed that the RSD values were all less than 2%, so the precision of the instrument was good.

3.7.7 Stability test

Take 10ul of polygala saponin reference substance and polygala test sample under item 3.7.3, and inject samples at 0, 2, 4, 8, 12, and 24 hours respectively according to the chromatographic conditions under item 3.7.2. The peak area was obtained, and the RSD value of the peak area was calculated. The results are shown in Tables 26 and 27.

Table 26 Stability test of polygala saponin reference substance

time (h) 0 2 4 8 12 twenty four RSD (%) Polygala saponin 874684.4 903083.7 871134.8 885089.9 875222.3 879744.8 1.32

Table 27 Polygala test product stability test

time (h) 0 2 4 8 12 twenty four RSD (%) Polygala saponin 471822.2 464063.7 471072.4 466371.6 472512.8 466072.6 0.76

From the results, it can be seen that the RSDs were all less than 2%, and the solutions of polygala saponin reference substance and test solution were basically stable within 24 hours.

3.7.8 Repeatability test

Prepare 6 samples in parallel according to the preparation of Polygala test sample under item 3.7.3, take 10ul for each, and inject samples according to the chromatographic conditions under item 3.7.2. The peak area was obtained, and the RSD value of the peak area was calculated. The results are shown in Table 28.

Table 28 Polygala test product repeatability test

serial number 1 2 3 4 5 6 RSD (%) Polygala saponin 446933.7 481060.1 471822.2 470000.2 467599.0 472454.0 2.44

The results showed that the RSD values were all less than 3%, and the repeatability of the sample preparation method was good.

3.7.9 Sample recovery test

Parallel precision measure 5ml of Polygala water decoction same as 6 parts for content determination, add appropriate amount of Polygala saponin reference substance, and prepare according to the preparation method of the test sample under 3.7.3. Take 10ul from each portion and inject according to the chromatographic conditions under 3.7.2. The recovery rate and RSD value were calculated, and the obtained results are shown in Table 29 below.

Table 29 Polygala saponin sample recovery test

The results showed that the recovery rate was in the range of 95%-105%, and the RSD was less than 3%. The sample preparation method is suggested to be feasible.

3.7.10 Determination results of the content of polygala saponin in each medicinal solution

According to the sample preparation method in 3.7.3, prepare 3 copies of polygala 20-minute medicinal liquid and 5 test samples in parallel, and inject 10ul of sample according to chromatographic conditions in 3.7.2, and inject 3 times in parallel for each sample. The average content and RSD value of polygala saponin in the medicinal liquid were determined. The results obtained are shown in Table 30, and the chromatograms of the Polygala 20-minute liquid medicine and the processed product 5 polygala saponins are shown in Figures 10 and 11.

Table 30 Determination results of polygala saponin content

The results showed that the RSDs were all within 3%. Compared with Polygala 20-minute medicinal solution in processed product 5, the content of Polygala saponin was slightly increased.

Different processing and decocting processes were designed by orthogonal tables, and the processing technology that can reduce the side effects of Polygala gastrointestinal motility inhibition was selected by gastric residual rate and small intestine charcoal propulsion experiments; pharmacological experiments were used to investigate the sedative and antitussive effects of each processed product. To verify its medicinal effect; use HPLC method to compare and measure the content changes of Polygala, Magnolia officinalis and their preferred processed products.

Results: ① Among the processed products, processed product 5 could significantly reduce the gastric residual rate and increase the intestinal propulsion rate in mice compared with the single-flavor polygala group decocted for 20 minutes and 60 minutes (P<0.05). ② The optimized processed product group 5 can significantly enhance the synergistic sedative effect of pentobarbital sodium (P<0.01), and each dose group can also significantly reduce the autonomous activity rate of mice (P<0.01, P<0.05) ; and can significantly prolong the cough incubation period of cough caused by ammonia water in mice (P<0.01), reduce the number of coughs (P<0.01), and its 30g/kg group can significantly increase the absorbance and concentration of phenol red in the trachea of mice ( P<0.05) and has an expectorant effect. ③The average contents of honokiol and magnolol in the single-flavored magnolia decoction group for 10 minutes were 0.100 and 0.153 mg/g, respectively, and the processed product 5 were 0.073 and 0.111 mg/g respectively; The content of polygala saponin is 2.650mg/g, and that of processed product 5 is 3.840mg/g.

Magnolia Radix Polygalae processed product of the present invention finally selects and adopts the following method to prepare:

①Preparation of magnolia juice: Take 70-130g of magnolia medicinal material, soak it in 8-12 times the amount of water for 15-45min, boil it with strong fire, then decoct it with slow fire for 5-15min, and concentrate it under reduced pressure to 10-40ml to get magnolia concentrated juice.

② Sunburn Polygala with Magnolia Juice: Take 30-70g of Polygala medicinal material, add 10-40ml concentrated Magnolia officinalis juice to moisten for 1-5h, stir-fry at 60-180°C for 6-18min, and dry it.

Processed product 5, the preferred preparation process of Magnolia officinalis Radix Polygalae processed product of the present invention is as follows:

①Preparation of Magnolia officinalis juice: Take 100g of Magnolia officinalis medicinal material, soak it in 8-12 times the amount of water for 30 minutes, boil it with strong fire, then decoct it with slow fire for 10 minutes, and concentrate it under reduced pressure to 25ml, which is the concentrated Magnolia bark juice.

②Steaming Polygala with Magnolia Juice: Take 50g of Polygala medicinal material, add 25ml of concentrated Magnolia officinalis juice and moisten for 3 hours, fry and broil at 120°C for 12 minutes, and dry it.

The decocting conditions of the processed product of the present invention: take the Magnolia officinalis Juice prepared by the above method and roast Polygala (theoretical crude drug amount is 150g), add 8-12 times of water to soak for 15-45min, boil with strong fire, decoct with slow fire for 10-30min, concentrate under reduced pressure, Set the volume to 100ml, which is equivalent to 1.5g/ml of the total amount of raw medicinal materials.

The optimal decocting conditions are: take Magnolia officinalis Juice Zhigala (theoretical crude drug amount 150g) prepared by the above method, add 10 times of water to soak for 30 minutes, boil it with strong fire, then decoct it with slow fire for 20 minutes, concentrate under reduced pressure, and set the volume to 100ml, which is equivalent to containing The total amount of raw medicinal materials is 1.5g/ml.

The processed decoction pieces of Polygala prepared under the above conditions can effectively reduce the gastrointestinal motility inhibitory side effects of Polygala polygala, and can retain the effects of antitussive, expectorant, and tranquilizing drugs, achieving the purpose of reducing side effects and saving efficacy, and has clinical application value.

Claims (4)

1. The processed product of Magnolia officinalis Radix Polygalae, which is characterized in that: Radix Polygalae is soaked in Magnolia officinalis juice and then fried and broiled; Wherein, the preparation method comprises the following steps: A. Magnolia officinalis and polygala according to crude drug ratio: 1 part of Magnolia officinalis, 1 part of polygala; B. Preparation of magnolia bark juice: take the magnolia bark medicinal material and add water to decoct for 5 minutes, and filter to obtain magnolia bark juice; according to 50 g of polygala crude drug, step B concentrates the filtered magnolia bark juice into 10 ml; C. Sunburn Polygala with Magnolia Juice: Soak Polygala Magnolia in the concentrated Magnolia juice obtained in step B until Polygala absorbs the concentrated Magnolia Bark juice, stir-fry at 60°C for 6 minutes, after the juice is collected, dry to obtain the processed Magnolia Polygala; or A. Magnolia officinalis and polygala according to crude drug ratio: 1 part of Magnolia officinalis, 1 part of polygala; B. Preparation of magnolia bark juice: take the magnolia bark medicinal material and add water to decoct for 10 minutes, and filter to obtain magnolia bark juice; according to 50 g of polygala crude drug, step B concentrates the filtered magnolia bark juice into 25 ml; C. Sunburn Polygala with Magnolia Juice: Soak Polygala with Magnolia Concentrated Juice obtained in Step B, until Polygala absorbs Concentrated Magnolia Juice, fry and roast at 90°C for 9 minutes, after the juice is collected, dry to obtain processed Magnolia and Polygala; or A. Magnolia officinalis and polygala according to crude drug ratio: 2 parts of Magnolia officinalis, 1 part of polygala; B. Preparation of magnolia bark juice: take the magnolia bark medicinal material and add water to decoct for 5 minutes, and filter to obtain magnolia bark juice; according to 50 g of polygala crude drug, step B concentrates the filtered magnolia bark juice into 10 ml; C. Sunburn Polygala with Magnolia Juice: Soak Polygala with Magnolia Concentrated Juice obtained in Step B, until Polygala absorbs Concentrated Magnolia Juice, fry and roast at 90°C for 9 minutes, after the juice is collected, dry to obtain processed Magnolia and Polygala; or A. Magnolia officinalis and polygala according to crude drug ratio: 2 parts of Magnolia officinalis, 1 part of polygala; B. Preparation of magnolia bark juice: take the magnolia bark medicinal material and add water to decoct for 10 minutes, and filter to obtain magnolia bark juice; according to 50 g of polygala crude drug, step B concentrates the filtered magnolia bark juice into 25 ml; C. Sunburn Polygala with Magnolia Juice: Soak Polygala Magnoliae in the concentrated Magnolia officinale juice obtained in step B until the Polygala absorbs the concentrated Magnolia officinalis juice, fry and roast at 120°C for 12 minutes, and after the juice is collected, dry to obtain the processed Magnolia Radix Polygalae; or A. Magnolia officinalis and polygala according to crude drug ratio: 2 parts of Magnolia officinalis, 1 part of polygala; B. Preparation of magnolia bark juice: take the magnolia bark medicinal material and add water to decoct for 15 minutes, and filter to obtain magnolia bark juice; according to 50 g of polygala crude drug, step B concentrates the filtered magnolia bark juice into 40 ml; C. Sunburn Polygala with Magnolia Juice: Soak Polygala Magnolia in the concentrated Magnolia juice obtained in step B until Polygala absorbs the concentrated Magnolia Bark juice, stir-fry at 60°C for 6 minutes, after the juice is collected, dry to obtain the processed Magnolia Polygala; or A. Magnolia officinalis and polygala according to crude drug ratio: 1 part of Magnolia officinalis, 1 part of polygala; B. Preparation of magnolia bark juice: take the magnolia bark medicinal material and add water to decoct for 15 minutes, and filter to obtain magnolia bark juice; according to 50 g of polygala crude drug, step B concentrates the filtered magnolia bark juice into 25 ml; C. Sunburn Polygala with Magnolia Juice: Soak Polygala Magnoliae in the concentrated Magnolia officinale juice obtained in step B until the Polygala absorbs the concentrated Magnolia officinalis juice, fry and roast at 90°C for 6 minutes, after the juice is collected, dry to obtain the processed product of Magnolia Polygala Magnolia; or A. Magnolia officinalis and polygala according to crude drug ratio: 2 parts of Magnolia officinalis, 1 part of polygala; B. Preparation of magnolia bark juice: take the magnolia bark medicinal material and add water to decoct for 5 minutes, and filter to obtain magnolia bark juice; according to 50 g of polygala crude drug, step B concentrates the filtered magnolia bark juice into 25 ml; C. Sunburn Polygala with Magnolia Juice: Soak Polygala with Magnolia Concentrated Juice obtained in Step B, until Polygala absorbs the Concentrated Magnolia Juice, fry and roast at 120°C for 6 minutes, after the juice is collected, dry to obtain processed Magnolia and Polygala; or A. Magnolia officinalis and polygala according to crude drug ratio: 2 parts of Magnolia officinalis, 1 part of polygala; B. Preparation of magnolia bark juice: take the magnolia bark medicinal material and add water to decoct for 10 minutes, and filter to obtain magnolia bark juice; according to 50 g of polygala crude drug, step B concentrates the filtered magnolia bark juice into 40 ml; C. Sunburn Polygala with Magnolia Juice: Soak Polygala Magnolia in the concentrated Magnolia juice obtained in step B until Polygala absorbs the concentrated Magnolia officinale juice, fry and roast at 60°C for 9 minutes, after the juice is collected, dry to obtain the processed Magnolia Radix Polygala; or A. Magnolia officinalis and polygala according to crude drug ratio: 3 parts of Magnolia officinalis, 1 part of polygala; B. Preparation of magnolia bark juice: take the magnolia bark medicinal material and add water to decoct for 5 minutes, and filter to obtain magnolia bark juice; according to 50 g of polygala crude drug, step B concentrates the filtered magnolia bark juice into 40 ml; C. Sunburn Polygala with Magnolia Juice: Soak Polygala with Magnolia Concentrated Juice obtained in Step B, until Polygala absorbs concentrated Magnolia Juice, fry and roast at 90°C for 12 minutes, after the juice is collected, dry to obtain processed Magnolia Polygala. 2. the preparation method of Magnolia officinalis Radix Polygalis processed product described in claim 1, is characterized in that: it comprises the steps: A. Magnolia officinalis and polygala according to crude drug ratio: 1 part of Magnolia officinalis, 1 part of polygala; B. Preparation of magnolia bark juice: take the magnolia bark medicinal material and add water to decoct for 5 minutes, and filter to obtain magnolia bark juice; according to 50 g of polygala crude drug, step B concentrates the filtered magnolia bark juice into 10 ml; C. Sunburn Polygala with Magnolia Juice: Soak Polygala Magnoliae in the concentrated Magnolia officinalis juice obtained in step B until the Polygala absorbs the concentrated Magnolia officinalis juice, fry and roast at 60°C for 6 minutes, after the juice is collected, dry to obtain the processed product of Magnolia and Polygala Magnolia; or A. Magnolia officinalis and polygala according to crude drug ratio: 1 part of Magnolia officinalis, 1 part of polygala; B. Preparation of magnolia bark juice: take the magnolia bark medicinal material and add water to decoct for 10 minutes, and filter to obtain magnolia bark juice; according to 50 g of polygala crude drug, step B concentrates the filtered magnolia bark juice into 25 ml; C. Sunburn Polygala with Magnolia Juice: Soak Polygala with Magnolia Concentrated Juice obtained in Step B, until Polygala absorbs Concentrated Magnolia Juice, fry and roast at 90°C for 9 minutes, after the juice is collected, dry to obtain processed Magnolia and Polygala; or A. Magnolia officinalis and polygala according to crude drug ratio: 2 parts of Magnolia officinalis, 1 part of polygala; B. Preparation of magnolia bark juice: take the magnolia bark medicinal material and add water to decoct for 5 minutes, and filter to obtain magnolia bark juice; according to 50 g of polygala crude drug, step B concentrates the filtered magnolia bark juice into 10 ml; C. Sunburn Polygala with Magnolia Juice: Soak Polygala with Magnolia Concentrated Juice obtained in Step B, until Polygala absorbs Concentrated Magnolia Juice, fry and roast at 90°C for 9 minutes, after the juice is collected, dry to obtain processed Magnolia and Polygala; or A. Magnolia officinalis and polygala according to crude drug ratio: 2 parts of Magnolia officinalis, 1 part of polygala; B. Preparation of magnolia bark juice: take the magnolia bark medicinal material and add water to decoct for 10 minutes, and filter to obtain magnolia bark juice; according to 50 g of polygala crude drug, step B concentrates the filtered magnolia bark juice into 25 ml; C. Sunburn Polygala with Magnolia Juice: Soak Polygala Magnoliae in the concentrated Magnolia officinale juice obtained in step B until the Polygala absorbs the concentrated Magnolia officinalis juice, fry and roast at 120°C for 12 minutes, and after the juice is collected, dry to obtain the processed Magnolia Radix Polygalae; or A. Magnolia officinalis and polygala according to crude drug ratio: 2 parts of Magnolia officinalis, 1 part of polygala; B. Preparation of magnolia bark juice: take the magnolia bark medicinal material and add water to decoct for 15 minutes, and filter to obtain magnolia bark juice; according to 50 g of polygala crude drug, step B concentrates the filtered magnolia bark juice into 40 ml; C. Sunburn Polygala with Magnolia Juice: Soak Polygala Magnolia in the concentrated Magnolia juice obtained in step B until Polygala absorbs the concentrated Magnolia Bark juice, stir-fry at 60°C for 6 minutes, after the juice is collected, dry to obtain the processed Magnolia Polygala; or A. Magnolia officinalis and polygala according to crude drug ratio: 1 part of Magnolia officinalis, 1 part of polygala; B. Preparation of magnolia bark juice: take the magnolia bark medicinal material and add water to decoct for 15 minutes, and filter to obtain magnolia bark juice; according to 50 g of polygala crude drug, step B concentrates the filtered magnolia bark juice into 25 ml; C. Sunburn Polygala with Magnolia Juice: Soak Polygala Magnoliae in the concentrated Magnolia officinale juice obtained in step B until the Polygala absorbs the concentrated Magnolia officinalis juice, fry and roast at 90°C for 6 minutes, after the juice is collected, dry to obtain the processed product of Magnolia Polygala Magnolia; or A. Magnolia officinalis and polygala according to crude drug ratio: 2 parts of Magnolia officinalis, 1 part of polygala; B. Preparation of magnolia bark juice: take the magnolia bark medicinal material and add water to decoct for 5 minutes, and filter to obtain magnolia bark juice; according to 50 g of polygala crude drug, step B concentrates the filtered magnolia bark juice into 25 ml; C. Sunburn Polygala with Magnolia Juice: Soak Polygala with Magnolia Concentrated Juice obtained in Step B, until Polygala absorbs the Concentrated Magnolia Juice, fry and roast at 120°C for 6 minutes, after the juice is collected, dry to obtain processed Magnolia and Polygala; or A. Magnolia officinalis and polygala according to crude drug ratio: 2 parts of Magnolia officinalis, 1 part of polygala; B. Preparation of magnolia bark juice: take the magnolia bark medicinal material and add water to decoct for 10 minutes, and filter to obtain magnolia bark juice; according to 50 g of polygala crude drug, step B concentrates the filtered magnolia bark juice into 40 ml; C. Sunburn Polygala with Magnolia Juice: Soak Polygala Magnolia in the concentrated Magnolia juice obtained in step B until Polygala absorbs the concentrated Magnolia officinale juice, fry and roast at 60°C for 9 minutes, after the juice is collected, dry to obtain the processed Magnolia Radix Polygala; or A. Magnolia officinalis and polygala according to crude drug ratio: 3 parts of Magnolia officinalis, 1 part of polygala; B. Preparation of magnolia bark juice: take the magnolia bark medicinal material and add water to decoct for 5 minutes, and filter to obtain magnolia bark juice; according to 50 g of polygala crude drug, step B concentrates the filtered magnolia bark juice into 40 ml; C. Sunburn Polygala with Magnolia Juice: Soak Polygala with Magnolia Concentrated Juice obtained in Step B, until Polygala absorbs concentrated Magnolia Juice, fry and roast at 90°C for 12 minutes, after the juice is collected, dry to obtain processed Magnolia Polygala. 3. The preparation method of Magnolia officinalis Radix Polygalis processed product according to claim 2, characterized in that: in step B, the time for adding water to soak Magnolia officinalis before decocting is 15-45min. 4. The preparation method of Magnolia officinalis Radix Polygalis processed product according to claim 3, characterized in that: in step B, the time for soaking Magnolia officinalis in water before decocting is 30 minutes.