CN102919183B - Method for effectively inhibiting vibrio breeding in shrimp culture - Google Patents
Method for effectively inhibiting vibrio breeding in shrimp culture Download PDFInfo
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- CN102919183B CN102919183B CN201210485397.9A CN201210485397A CN102919183B CN 102919183 B CN102919183 B CN 102919183B CN 201210485397 A CN201210485397 A CN 201210485397A CN 102919183 B CN102919183 B CN 102919183B
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- 241000607598 Vibrio Species 0.000 title claims abstract description 65
- 241000238557 Decapoda Species 0.000 title claims abstract description 47
- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000009395 breeding Methods 0.000 title claims abstract description 20
- 230000001488 breeding effect Effects 0.000 title claims abstract description 20
- 230000002401 inhibitory effect Effects 0.000 title abstract 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 35
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 19
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 19
- 206010047400 Vibrio infections Diseases 0.000 claims description 49
- 238000012258 culturing Methods 0.000 claims description 14
- 230000005764 inhibitory process Effects 0.000 claims description 11
- 239000004459 forage Substances 0.000 claims description 9
- 230000003203 everyday effect Effects 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 238000005273 aeration Methods 0.000 claims description 6
- 235000012054 meals Nutrition 0.000 claims description 6
- 230000004083 survival effect Effects 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000003814 drug Substances 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 5
- 241000238553 Litopenaeus vannamei Species 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- 239000000460 chlorine Substances 0.000 claims description 3
- 229910052801 chlorine Inorganic materials 0.000 claims description 3
- 238000007796 conventional method Methods 0.000 claims description 3
- 230000036039 immunity Effects 0.000 claims description 3
- 235000013379 molasses Nutrition 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 claims description 3
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 abstract description 25
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 5
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 5
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 239000002207 metabolite Substances 0.000 abstract description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 7
- 201000010099 disease Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 241000544286 Vibrio anguillarum Species 0.000 description 3
- 238000009360 aquaculture Methods 0.000 description 3
- 244000144974 aquaculture Species 0.000 description 3
- 239000013535 sea water Substances 0.000 description 3
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- 241000251468 Actinopterygii Species 0.000 description 2
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- 206010059866 Drug resistance Diseases 0.000 description 2
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- 235000017845 Persicaria vulgaris Nutrition 0.000 description 2
- 244000170788 Persicaria vulgaris Species 0.000 description 2
- 241000607594 Vibrio alginolyticus Species 0.000 description 2
- 229930003268 Vitamin C Natural products 0.000 description 2
- 230000002180 anti-stress Effects 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000000091 immunopotentiator Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 210000000496 pancreas Anatomy 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
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- 239000011718 vitamin C Substances 0.000 description 2
- 241000607534 Aeromonas Species 0.000 description 1
- 208000031729 Bacteremia Diseases 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
- 240000004859 Gamochaeta purpurea Species 0.000 description 1
- 241000927735 Penaeus Species 0.000 description 1
- 241000607493 Vibrionaceae Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
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- 210000001630 jejunum Anatomy 0.000 description 1
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- 150000003892 tartrate salts Chemical class 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a method for effectively inhibiting vibrio breeding in shrimp culture. According to the method, an organic carbon substance is artificially added, the carbon to nitrogen (C/N) ratio in a water body is adjusted, the amount of heterotrophic bacteria in a water environment is increased, microbes are used for assimilating inorganic nitrogen to convert culture metabolites such as ammonia nitrogen in the water body into self components of bacteria, the self components of bacteria are flocculated into particulate matters by the bacteria, and the particulate matters are ingested by cultured organisms, so that the effect of effectively inhibiting vibrio breeding in shrimp culture is achieved.
Description
Technical field
The present invention relates to aquaculture field, exactly refer to the method for vibrios breeding in a kind of effective inhibition prawn culturing.
Background technology
Therefore vibrios (vibrio) is one of bacterium monoid common in marine environment, and the adaptability that this bacterioid tool is very strong and resistance become the dominant population of briny environment, especially with vibrio alginolyticus, vibrio parahaemolytious, Vibrio anguillarum etc., preponderate.At present, the 9th edition < < uncle Jie Shi bacteriology handbook > > included 35 kinds of vibrio bacteriums.Along with propagating artificially, develop rapidly, cultivation waters ecological change, vibriosis has become one of main bacterial disease of marine cultured animal.
By the bacterial vibriosis of vibrio (Vibrio) (Vibriosis), be all over the world, cultivate generally popular in the aquatic livestocks such as fish, shrimp, crab and shellfish and endanger maximum bacteriosis, to culture fishery, caused serious economic loss.It is reported, Vibrio anguillarum in vibrio, vibrio parahaemolytious etc. are preponderated in vibrios population, extensively be present in nature seawater, cause vibriosis to occur in the whole world, and have popular wide, the incidence of disease is high, harm is large, lethality high, to the cultivation of the seawater animals such as fish, shrimp, crab and shellfish, has caused tremendous influence.
The disease general designation vibriosis being caused by Vibrio anguillarum, extra large vibrios, vibrio alginolyticus and vibrio parahaemolytious.The common bacteriosis of prawn has prawn young bacteremia, fin rot, red leg disease, rotten illness in eye etc., its main pathogen is vibrios, pseudomonad, Aeromonas etc., wherein vibrionaceae is permitted various bacteria, it is the important pathogen that causes prawn bacterioid disease, and as the flora of being everlasting in seawater, vibrios is also present in the body of healthy shell-fish individuality, and Gomez etc. have reported in the liver pancreas of Penaeus vannamei may exist Vibrio.In water, the quantity of vibrios is 10
3-10
4cFU/mL(CFU/mL: in the time of the bacterial community sum containing in every ml sample), the red leg disease symptom of prawn obviously increases the weight of.By contrast, find out, in the occurring degree of prawn and death condition and shrimp pool water, the quantity of vibrios has certain relation.Have research to think, the amount of vibrio in water reaches 10
4during CFU/mL, prawn just may infected morbidity.
In the middle of production, owing to needing the quantity of fast detecting vibrios, can often utilizing the sucrose in medium to be divided into, vibrios that can decomposing sucrose presents yellow, and vibrios that can not decomposing sucrose presents green, and being simply divided into is yellow bacterium, green bacterium and fluorescens strain.
At present, the traditional method of control vibriosis penaeus can take the measures such as water body disinfection, water quality and substrate improvement, endo-medicine and interpolation immunopotentiator to prevent, the first-selection that antibiotic is treated often, the biologic products such as bacillus are also the common methods of control vibrios.The method of sterilization often allows vibrios bounce-back more severe, and the sudden change that also has some drug resistance strains makes Disinfection Effect poor time by time.Water quality and substrate improvement are mainly to start with from water quality physical and chemical factor aspect, often cure the symptoms, not the disease; Immunopotentiator for oral administration is also lost in water because the characteristic of ingesting of shrimp causes part, and really entering in shrimp body is also a unknown number; Antibiotic use not only causes the pesticide resistance of bacterium, and, along with more and more the coming into one's own and be eliminated at last of food security, so the breeding of this method control vibrios has environmental protection, green, efficiently, also can not produce the features such as drug resistance.
Summary of the invention
For above-mentioned defect, the technical problem that the present invention solves is to provide the method for vibrios breeding in a kind of effective inhibition prawn culturing, can effectively suppress the vibrios in prawn culturing.
In order to solve above technical problem, the method for vibrios breeding in effective inhibition prawn culturing provided by the invention, comprises the following steps:
(1), arrange, sterilize, sterilization after culture pond arranges; Be exposed to the sun in the empty pool, then available chlorine 25g/m is used in water inlet
3concentration process more than 6 hours, aeration;
(2), adjust the C/N ratio of water body, aeration, adds carbon source 8g/m every day
3, protein content is at more than 45% prawn slice 4g/m
3, the DO of water body is not less than 5～6g/m
3;
(3), after 4～5 days, transparency, at 40～50cm, can be tried water and put seedling, 24 hours, survival rate more than 95% can put seedling, putting seedling density can 1000～10000/square metre depending on hardware condition difference;
(4), regulate C/N ratio, the content of monitoring vibrios, when the quantity of vibrios surpasses 4 orders of magnitude, the addition of carbon is brought up to the forage volume of throwing something and feeding 1.2～1.5 times; If amount of vibrio is when 2～4 orders of magnitude, the addition of carbon is 1.0～1.2 times of forage volume of throwing something and feeding; If amount of vibrio at 2 below the order of magnitude and luminous Vibrio be 0 o'clock, the addition of carbon be throw something and feed 0.5 times of forage volume following;
(5), cultivation 30 days, seedling specification, at 0.3～1.0g/ tail, can divide seedling to culture pond, culture pond is adjusted C/N ratio according to detecting vibrios content equally, greatly improves cultivation success rate;
(6), by conventional method, cultivate after dividing seedling:
A, every day are according to the situation of the ingesting increase and decrease inventory of shrimp;
B, every seven days supplementary bacillus;
Before c, Changes in weather, before bad weather arrives, spill anti-stress vitamin C 3～5g/m
3.
Dividing the culture pond before seedling is the thick pool of mark, and the culture pond after minute seedling is for dividing a seedling pool.
Preferably, in step (2), carbon source is a kind of or combination in brown sugar, molasses or glucose, and nitrogenous source is the thick special-purpose prawn slice of ecological mark.
Preferably, in step (3), put seedling same day, throw special-purpose penaeus vannamei boone feed, 1 jin/100,000 tails, 6 meal, every four hours one meal, according to the situation of ingesting, feed in raw material 10～20% every day.
Preferably, in step (5), the preparation on minute seedling pool is identical before putting seedling with the thick pool of mark, divide the He Biaocu pool, seedling pool exchanged water to dwindle the gap between two pools, first 5 days of minute seedling, marks the thick pool shrimp seedling medicine that improves immunity for oral administration, within first 1 day, stop material, select the shelling interval of shrimp to divide seedling.
Preferably, the salinity range of marking water in the thick pool is 3 ‰-30 ‰.
Compared with prior art, in effective inhibition prawn culturing provided by the invention, the method for vibrios breeding, can effectively suppress the vibrios in prawn culturing.
Embodiment
For those skilled in the art can understand technical scheme provided by the present invention better, below in conjunction with specific embodiment, set forth.
The method of vibrios breeding in effective inhibition prawn culturing that the embodiment of the present invention provides, comprises the following steps:
(1), arrange, sterilize, sterilization after culture pond arranges; Be exposed to the sun in the empty pool, then available chlorine 25g/m is used in water inlet
3concentration process more than 6 hours, aeration;
(2), adjust the C/N ratio of water body, aeration, adds carbon source 8g/m every day
3, protein content is at more than 45% prawn slice 4g/m
3, the DO of water body is not less than 5～6g/m
3;
(3), after 4～5 days, transparency, at 40～50cm, can be tried water and put seedling, 24 hours, survival rate more than 95% can put seedling, putting seedling density can 1000～10000/square metre depending on hardware condition difference;
(4), regulate C/N ratio, the content of monitoring vibrios, when the quantity of vibrios surpasses 4 orders of magnitude, the addition of carbon is brought up to the forage volume of throwing something and feeding 1.2～1.5 times; If amount of vibrio is when 2～4 orders of magnitude, the addition of carbon is 1.0～1.2 times of forage volume of throwing something and feeding; If amount of vibrio at 2 below the order of magnitude and luminous Vibrio be 0 o'clock, the addition of carbon be throw something and feed 0.5 times of forage volume following;
(5), cultivation 30 days, seedling specification, at 0.3～1.0g/ tail, can divide seedling to culture pond, culture pond is adjusted C/N ratio according to detecting vibrios content equally, greatly improves cultivation success rate;
(6), by conventional method, cultivate after dividing seedling:
A, every day are according to the situation of the ingesting increase and decrease inventory of shrimp;
B, every seven days supplementary bacillus;
Before c, Changes in weather, before bad weather arrives, spill anti-stress vitamin C 3～5g/m
3.
In step (2), carbon source is a kind of or combination in brown sugar, molasses or glucose, and nitrogenous source is the thick special-purpose prawn slice of ecological mark.
In step (3), put seedling same day, throw special-purpose penaeus vannamei boone feed, 1 jin/100,000 tails, 6 meal, every four hours one meal, according to the situation of ingesting, feed in raw material 10～20% every day.
In step (5), the preparation on minute seedling pool is identical before putting seedling with the thick pool of mark, and minute He Biaocu pool, seedling pool exchanged water is to dwindle the gap between two pools, divide seedling first 5 days, mark for oral administration medicine that improves immunity of thick pool shrimp seedling, within first 1 day, stop material, select the shelling interval of shrimp to divide seedling.
The salinity range of marking water in the thick pool is 3 ‰-30 ‰, evidence within the scope of this, and the salinity of marking the water in the thick pool is higher, and the survival rate of shrimp seedling is also higher, and effect is better.
As follows after arranging:
Through significance analysis, from specification, success rate, survival rate, four indexs of feed coefficient, glucose and brown sugar all difference are not remarkable, from specification glucose and brown sugar, are less than control group, but success rate is much larger than control group, and difference is (p < 0.01) extremely significantly; Survival rate surpasses 30% higher than control group, significant difference; But feed coefficient three difference is little.
The thick method of the ecological mark of 1-6,7-12 is traditional cultural method: sterilization in 7 days once, the removing toxic substances medicine of the lower tartaric acid class in latter 48 hours of sterilizing, 48 hours lower bacillus biologic products.As can be seen from the above table, in traditional cultivation way, after sterilization, vibrios can reach a relatively low level, but because time of a vibrios breeding generation is than short many of the time of bacillus, so it is bad to utilize bacillus to suppress the effect of vibrios, some water body is bad control, be easy to allow vibrios become the breeding that dominant population has suppressed bacillus, corrode prawn, cause and cultivate unsuccessfully, as 9,10,12 when cultivating by 14～15 days, vibrios amount reproduction, considerably beyond 10
4, in prawn body, surpassed especially 10
7, caused prawn liver pancreas impaired, the empty stomach of jejunum, cultivates unsuccessfully.But with 3 pool cultivation failure in 23 days of this method, because negligent supervision DO is low to be caused, not in discussion scope.
The theory support of the method for vibrios breeding in effective inhibition prawn culturing that the embodiment of the present invention provides:
This method originates from the activated sludge process of urban sewage, is the inorganic nitrogen assimilation process of microorganism, and ammonia nitrogen etc. is changed into bacterial components.According to formula: NH4
++ 1.18C
6h
12o
6+ HCO
3 –+ 2.06O
2→ C
5h
7o
2n+6.06H
2o+3.07CO
2known, the mineralized nitrogen of every g is the dissolved oxygen that bacterium need to consume 4.71g, 3.57g basicity (0.86g DIC) and 15.17g carbohydrate (6.07g organic carbon).Reaction can generate the bacterium living beings body (4.29g organic carbon) of 8.07g and the carbonic acid gas (DIC of 2.63g) of 9.65g.The present invention is by the organic carbonizable substance of artificial interpolation, regulate the C/N ratio of water body, improve the quantity of heterotrophic bacteria in water environment, utilize microbial assimilation inorganic nitrogen, the cultivation metabolites such as ammonia nitrogen in water body are changed into bacterium self component, and by bacterium, flocculate into particulate matter and ingested by aquaculture organism, play the effect of regulation and control water quality, promotion Cycle of nutrients, reduction feed coefficient, raising aquaculture organism survival rate.Research shows, biological flocculation is the bacterium granule that is mutually flocculated and formed by bacterial community, plankton, organic debris and some polymeric materials.
It is generally acknowledged that the C/N ratio in bacterial cell is about 5, the C/N in raising pond waters is than being conducive to the breeding of bacterium self; And Chi Shuizhong to be take certainly nourish one's nature bacterium be main system transition as heterotrophic bacteria be main system, simultaneously can be by heterotrophic bacteria sum from 10
4cFU/mL brings up to 10
7cFU/mL.The raising of heterotrophic bacteria quantity, can effectively suppress the breeding of vibrios.C/N in raising pond waters is than being conducive to the breeding of bacterium self; And Chi Shuizhong to be take certainly nourish one's nature bacterium be main system transition as heterotrophic bacteria be main system, can be by heterotrophic bacteria sum from 10
4cFU/mL brings up to 10
7cFU/mL, effectively suppresses vibrios, growing of green bacterium and fluorescens strain particularly, thus greatly reduce the infringement of prawn body.
Above-mentioned explanation to the disclosed embodiments, makes professional and technical personnel in the field can realize or use the present invention.To the multiple modification of these embodiment, will be apparent for those skilled in the art, General Principle as defined herein can, in the situation that not departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention will can not be restricted to these embodiment shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (5)
1. effectively suppress a method for vibrios breeding in prawn culturing, it is characterized in that, comprise the following steps:
(1), arrange, sterilize, sterilization after culture pond arranges; Be exposed to the sun in the empty pool, then available chlorine 25g/m is used in water inlet
3concentration process more than 6 hours, aeration;
(2), adjust the C/N ratio of water body, aeration, adds carbon source 8g/m every day
3, protein content is at more than 45% prawn slice 4g/m
3, the DO of water body is not less than 5～6g/m
3;
(3), after 4～5 days, transparency, at 40～50cm, can be tried water and put seedling, 24 hours, survival rate more than 95% can put seedling, putting seedling density can 1000～10000/square metre depending on hardware condition difference;
(4), regulate C/N ratio, the content of monitoring vibrios, when the quantity of vibrios surpasses 4 orders of magnitude, the addition of carbon is brought up to the forage volume of throwing something and feeding 1.2～1.5 times; If amount of vibrio is when 2～4 orders of magnitude, the addition of carbon is 1.0～1.2 times of forage volume of throwing something and feeding; If amount of vibrio at 2 below the order of magnitude and luminous Vibrio be 0 o'clock, the addition of carbon be throw something and feed 0.5 times of forage volume following;
(5), cultivation 30 days, seedling specification, at 0.3～1.0g/ tail, can divide seedling to culture pond, culture pond is adjusted C/N ratio according to detecting vibrios content equally;
(6), by conventional method, cultivate after dividing seedling;
Dividing the culture pond before seedling is the thick pool of mark, and the culture pond after minute seedling is for dividing a seedling pool.
2. the method for vibrios breeding in effective inhibition prawn culturing according to claim 1, is characterized in that, in step (2), carbon source is a kind of or combination in brown sugar, molasses or glucose, and nitrogenous source is the thick special-purpose prawn slice of ecological mark.
3. the method for vibrios breeding in effective inhibition prawn culturing according to claim 1, is characterized in that, in step (3), puts seedling same day, throw special-purpose penaeus vannamei boone feed, 1 jin/100,000 tails, 6 meal, every four hours one meal, according to the situation of ingesting, every day reinforced 10～20%.
4. the method that in effective inhibition prawn culturing according to claim 1, vibrios breeds, it is characterized in that, in step (5), it is front identical that seedling is put on the preparation on minute seedling pool and the thick pool of mark, divide the He Biaocu pool, seedling pool exchanged water to dwindle the gap between two pools, first 5 days of minute seedling, marks the thick pool shrimp seedling medicine that improves immunity for oral administration, within first 1 day, stop material, select the shelling interval of shrimp to divide seedling.
5. the method for vibrios breeding in effective inhibition prawn culturing according to claim 1, is characterized in that, the salinity range of marking water in the thick pool is 3 ‰-30 ‰.
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|---|---|---|---|
| CN201210485397.9A CN102919183B (en) | 2012-11-23 | 2012-11-23 | Method for effectively inhibiting vibrio breeding in shrimp culture |
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|---|---|---|---|
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| CN102919183B true CN102919183B (en) | 2014-03-26 |
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| CN104770600A (en) * | 2015-04-10 | 2015-07-15 | 烟台大乐饲料有限公司 | Special penaeus orientalis functional biological feed in the thickness marking period |
| CN105210975B (en) * | 2015-11-05 | 2017-07-28 | 广东海洋大学 | The processing method in prawn Vibrio luminous disease pond |
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