CN102919044B - Cutting propagation method of industrial cannabis sativa - Google Patents
Cutting propagation method of industrial cannabis sativa Download PDFInfo
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- CN102919044B CN102919044B CN201210476676.9A CN201210476676A CN102919044B CN 102919044 B CN102919044 B CN 102919044B CN 201210476676 A CN201210476676 A CN 201210476676A CN 102919044 B CN102919044 B CN 102919044B
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- cuttage
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- 238000005520 cutting process Methods 0.000 title claims abstract description 39
- 244000025254 Cannabis sativa Species 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 23
- 235000008697 Cannabis sativa Nutrition 0.000 title abstract 4
- 239000011159 matrix material Substances 0.000 claims abstract description 15
- 241000196324 Embryophyta Species 0.000 claims abstract description 8
- 239000002985 plastic film Substances 0.000 claims abstract description 7
- 239000003415 peat Substances 0.000 claims abstract description 4
- 239000010451 perlite Substances 0.000 claims abstract description 4
- 235000019362 perlite Nutrition 0.000 claims abstract description 4
- 238000007789 sealing Methods 0.000 claims abstract description 4
- 239000002689 soil Substances 0.000 claims abstract description 4
- 235000013311 vegetables Nutrition 0.000 claims abstract description 4
- 239000010455 vermiculite Substances 0.000 claims abstract description 4
- 235000019354 vermiculite Nutrition 0.000 claims abstract description 4
- 229910052902 vermiculite Inorganic materials 0.000 claims abstract description 4
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 claims description 26
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 claims description 26
- 235000009120 camo Nutrition 0.000 claims description 26
- 235000005607 chanvre indien Nutrition 0.000 claims description 26
- 239000011487 hemp Substances 0.000 claims description 26
- 230000006378 damage Effects 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000010899 nucleation Methods 0.000 claims description 5
- 238000002054 transplantation Methods 0.000 claims description 4
- 238000009941 weaving Methods 0.000 claims description 4
- 244000198134 Agave sisalana Species 0.000 claims description 3
- 235000011624 Agave sisalana Nutrition 0.000 claims description 3
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 3
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 3
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 3
- 208000027418 Wounds and injury Diseases 0.000 claims description 3
- 239000011425 bamboo Substances 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 239000000417 fungicide Substances 0.000 claims description 3
- 208000014674 injury Diseases 0.000 claims description 3
- 230000003020 moisturizing effect Effects 0.000 claims description 3
- 239000005416 organic matter Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 238000013138 pruning Methods 0.000 claims description 3
- 230000005855 radiation Effects 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- FFSJPOPLSWBGQY-UHFFFAOYSA-N triazol-4-one Chemical compound O=C1C=NN=N1 FFSJPOPLSWBGQY-UHFFFAOYSA-N 0.000 claims description 3
- 244000082204 Phyllostachys viridis Species 0.000 claims 1
- 238000009395 breeding Methods 0.000 abstract description 9
- 230000001488 breeding effect Effects 0.000 abstract description 7
- 238000011160 research Methods 0.000 abstract description 5
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- 229920006255 plastic film Polymers 0.000 abstract 4
- 239000000835 fiber Substances 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 238000005286 illumination Methods 0.000 abstract 1
- 239000012286 potassium permanganate Substances 0.000 abstract 1
- 230000000644 propagated effect Effects 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 241001330002 Bambuseae Species 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000002411 adverse Effects 0.000 description 1
- 239000004566 building material Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000010159 dioecy Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000013332 literature search Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
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- Cultivation Of Plants (AREA)
Abstract
A cutting propagation method of industrial cannabis sativa belongs to the technical field of cultivation and breeding of bast fiber plants. The cutting propagation method comprises the following steps: performing final singling of seedlings of industrial cannabis sativa, wherein 1seedling is planted in one square meter; topping when the plant is 1m high and the primary branch length is 20cm, wherein the topping length is 2cm; uniformly mixing matrix and carrying out sterilization and disinfection, wherein the volume ratio of vegetable garden soil or cured loess to perlite or vermiculite to peat for planting in the matrix is 4:3:3; laying a layer of matrix which is 10cm thick on a seedbed which is 1.2m wide and 10m long; carrying out cuttage when branch shoots are 15-20cm long; preparing cutting shoots which are 10-15cm long and soaking the cutting shoots in a 1/5000 potassium permanganate solution for 20-30min, wherein only 2-3 leaves at the top of the cutting shoots which are not unfolded completely are kept, the cuttage density is 3-4cm*10-12cm and the cuttage depth is 3-5cm; setting up supports which are 50cm high, covering with a plastic film, sealing the periphery of the plastic film, performing proper shading, controlling the temperature inside the plastic film to be below 35 DEG C and the relative air humidity inside the plastic film to be higher than 85%; supplying illumination twice in morning and afternoon, 1-3 hours each time. By implementation of the cutting propagation method, 50-100 cuttage seedlings can be propagated from one seed (one seedling) after 90-120 days. The cutting propagation method is simple to operate, low in cost and high in seedling culture speed. The cutting propagation method is an optimal method for quickly obtaining seedlings with consistent genetic gene, can be widely applied to cultivation and breeding of industrial cannabis sativa and related researches.
Description
Technical field:
The present invention relates to a kind of method of industrial hemp cottage propagation, belong to crudefiber crop cultivation and breeding technical field.
Background technology:
The application of industrial hemp relates to ecological health food, auto industry, weaving, building materials, papermaking, military supplies and other light industry fields, and the recent domestic development rapidly.Industrial hemp is the dioecism cross-pollinatd plant, and in kind, interindividual variation is larger, and cultivation utilization and scientific experimentation are brought to adverse effect.The technology of the present invention is by a large amount of seedling of quickly breeding same gene type (individual plant source), and the research for different disposal on the hemp impact significantly improves reliability and the science of result of the test.
By literature search, have no the open report identical with the present invention.
Summary of the invention:
The object of the invention is to overcome the difference between existing industrial hemp kind individuality, a kind of method of industrial hemp cottage propagation is provided.
Research finds, industrial hemp after cuttings is cultivated under protective condition cottage propagation, through correct maintenance, cuttings started to take root after 10-15 days, within 25-30 days, played afterwards transplantation of seedlings or for the scientific experimentation of design.Set out by an industrial hemp seed, after 90-120 days, can obtain cuttage seeding 50-100.
Industrial hemp cuttage breeding method of the present invention, its concrete steps are as follows:
1. cuttings is cultivated the plantation of light seeding as early as possible hemp when temperature meets the hemp growth, final singling 1 strain/m
2, strengthen rich water quality management, promote numb strain growth, when numb plant height degree 1m, pinch, remove top tip 2cm, promote a minute branch growth; Remove again the top 2cm of branch when primary branch grows to 20cm, in this way on the hemp plant, obtain required minute branch.
2. matrix is prepared by removing not vegetable garden soil or the slaking loess of decomposed organic matter: perlite or vermiculite: cultivation mixes by the volume ratio of peat=4:3:3, every cube of matrix is used 200-300L soil fungicides triazolone or tmtd solution, concentration is 2 times of product description working concentration, carries out sterilizing.
3. the necessary draining in ground, preparing seedbed, seedbed is unobstructed; Seedbed width 1.2m, length≤10m; The thick matrix of upper berth, seedbed 10cm.
4. cuttings is prepared when a minute branch grows to 15-20cm length for cuttage and seedling culture; Use sharp scissors or single-edge blade to shear cuttings, length 10-15cm, retain top 2-3 the not yet blade of full extension, removes unnecessary blade and petiole, soaks 20-30 minute in 1/5000 liquor potassic permanganate; Avoid the damage to cuttings terminal bud and stem in cuttings pruning process.
5. cuttage technique cutting density 3-4cm * 10-12cm, insert dark 3-5cm; Cuttage is wanted to put in place for 1 time, plug repeatedly; After cuttage, water the liquor potassic permanganate that soaks cuttings, drenched matrix.
6. after inserting, thin bamboo strips used for weaving sheet or customization bow member are prepared in management in advance, support immediately after cuttage, and support height 50cm, cover transparent plastic film, surrounding compacting sealing, moisturizing; Use sunshade net covered with sunshade, cooling and the injury of minimizing high light radiation; Keep the interior relative air humidity of film more than 85%, temperature is no more than 35 ℃; Every day of fine day early, the period in evening throw off cover from sun make cuttings early, respectively accept sunlight 1-2 hour evening; Along with the growth of cuttings and adaptability strengthen, early, see that the time of light respectively extends to 2-3 hour evening.
7. within 10-15 days, start to take root after playing the cuttage of transplantation of seedlings hemp, continue to cultivate 10-15 days, balled transplanting or lifting are for scientific experimentation.
Through the cuttings of 90-120 days, cultivate and cuttage and seedling culture, can be by the consistent cuttage seeding 50-100 of a seed (1 seedling) breeding hereditary basis.
The invention has the advantages that:
1. can overcome the individual difference of industrial hemp seed shoots, a large amount of seedling of quickly breeding same gene type (individual plant source), the research for different disposal on the hemp impact, significantly improve reliability and the science of result of the test;
2. the method is simple to operate, and cost is low, grows seedlings fast, and growth is neat, can be widely used in industrial hemp cultivation, breeding and relevant scientific research.
Specific implementation method:
Industrial hemp cuttage breeding method of the present invention, its concrete steps are as follows:
1. cuttings is cultivated the plantation of light seeding as early as possible hemp when temperature meets the hemp growth, final singling 1 strain/m
2, strengthen rich water quality management, promote numb strain growth, when numb plant height degree 1m, pinch, remove top tip 2cm, promote a minute branch growth; Remove again the top 2cm of branch when primary branch grows to 20cm, in this way on the hemp plant, obtain required minute branch.
2. matrix is prepared by removing not vegetable garden soil or the slaking loess of decomposed organic matter: perlite or vermiculite: cultivation mixes by the volume ratio of peat=4:3:3, every cube of matrix is used 200-300L soil fungicides triazolone or tmtd solution, concentration is 2 times of product description working concentration, carries out sterilizing.
3. the necessary draining in ground, preparing seedbed, seedbed is unobstructed; Seedbed width 1.2m, length≤10m; The thick matrix of upper berth, seedbed 10cm.
4. cuttings is prepared when a minute branch grows to 15-20cm length for cuttage and seedling culture; Use sharp scissors or single-edge blade to shear cuttings, length 10-15cm, retain top 2-3 the not yet blade of full extension, removes unnecessary blade and petiole, soaks 20-30 minute in 1/5000 liquor potassic permanganate; Avoid the damage to cuttings terminal bud and stem in cuttings pruning process.
5. cuttage technique cutting density 3-4cm * 10-12cm, insert dark 3-5cm; Cuttage is wanted to put in place for 1 time, plug repeatedly; After cuttage, water the liquor potassic permanganate that soaks cuttings, drenched matrix.
6. after inserting, thin bamboo strips used for weaving sheet or customization bow member are prepared in management in advance, support immediately after cuttage, and support height 50cm, cover transparent plastic film, surrounding compacting sealing, moisturizing; Use sunshade net covered with sunshade, cooling and the injury of minimizing high light radiation; Keep the interior relative air humidity of film more than 85%, temperature is no more than 35 ℃; Every day of fine day early, the period in evening throw off cover from sun make cuttings early, respectively accept sunlight 1-2 hour evening; Along with the growth of cuttings and adaptability strengthen, early, see that the time of light respectively extends to 2-3 hour evening.
7. within 10-15 days, start to take root after playing the cuttage of transplantation of seedlings hemp, continue to cultivate 10-15 days, balled transplanting or lifting are for scientific experimentation.
The inventor breeds the method for industrial hemp.Facts have proved, the method is simple to operate, and cost is low, grows seedlings fast, and growth is neat, can effectively overcome the problem that the seminal propagation individual difference is large, is the method for optimizing that obtains fast the consistent industrial hemp seedling of hereditary basis.
Claims (1)
1. the method for an industrial hemp cottage propagation is characterized in that the concrete steps of the method are as follows:
A. cuttings is cultivated the plantation of light seeding as early as possible hemp when temperature meets the hemp growth, final singling 1 strain/m
2, strengthen rich water quality management, promote numb strain growth, when numb plant height degree 1m, pinch, remove top tip 2cm, promote a minute branch growth; Remove again the top 2cm of branch when primary branch grows to 20cm, in this way on the hemp plant, obtain required minute branch;
B. matrix is prepared by removing not vegetable garden soil or the slaking loess of decomposed organic matter: perlite or vermiculite: cultivation mixes by the volume ratio of peat=4:3:3, every cube of matrix is used 200-300L soil fungicides triazolone or tmtd solution, concentration is 2 times of product description working concentration, carries out sterilizing;
C. the necessary draining in ground, preparing seedbed, seedbed is unobstructed; Seedbed width 1.2m, length≤10m; The thick matrix of upper berth, seedbed 10cm;
D. cuttings is prepared when a minute branch grows to 15-20cm length for cuttage and seedling culture; Use sharp scissors or single-edge blade to shear cuttings, length 10-15cm, retain top 2-3 the not yet blade of full extension, removes unnecessary blade and petiole, soaks 20-30 minute in 1/5000 liquor potassic permanganate; Avoid the damage to cuttings terminal bud and stem in cuttings pruning process;
E. cuttage technique cutting density 3-4cm * 10-12cm, insert dark 3-5cm; Cuttage is wanted to put in place for 1 time, plug repeatedly; After cuttage, water the liquor potassic permanganate that soaks cuttings, drenched matrix;
F. after inserting, thin bamboo strips used for weaving sheet or customization bow member are prepared in management in advance, support immediately after cuttage, and support height 50cm, cover transparent plastic film, surrounding compacting sealing, moisturizing; Use sunshade net covered with sunshade, cooling and the injury of minimizing high light radiation; Keep the interior relative air humidity of film more than 85%, temperature is no more than 35 ℃; Every day of fine day early, the period in evening throw off cover from sun make cuttings early, respectively accept sunlight 1-2 hour evening; Along with the growth of cuttings and adaptability strengthen, early, see that the time of light respectively extends to 2-3 hour evening;
G. within 10-15 days, start to take root after playing the cuttage of transplantation of seedlings hemp, continue to cultivate 10-15 days, balled transplanting or lifting are for scientific experimentation.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210476676.9A CN102919044B (en) | 2012-11-22 | 2012-11-22 | Cutting propagation method of industrial cannabis sativa |
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| CN201210476676.9A CN102919044B (en) | 2012-11-22 | 2012-11-22 | Cutting propagation method of industrial cannabis sativa |
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| Publication Number | Publication Date |
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| CN102919044A CN102919044A (en) | 2013-02-13 |
| CN102919044B true CN102919044B (en) | 2014-01-08 |
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| CN201210476676.9A Expired - Fee Related CN102919044B (en) | 2012-11-22 | 2012-11-22 | Cutting propagation method of industrial cannabis sativa |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110839418A (en) * | 2019-11-28 | 2020-02-28 | 黑龙江雪域安康工业大麻科技有限责任公司 | Vegetative propagation method of industrial cannabis sativa L |
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| CN103416206B (en) * | 2013-08-20 | 2015-05-20 | 山西省农业科学院经济作物研究所 | Improved variety breeding technology for industrial cannabis sativa L. |
| CN105230465A (en) * | 2015-11-10 | 2016-01-13 | 云南大学 | Industrial marijuana soilless rapid propagation method |
| CN105284391B (en) * | 2015-11-20 | 2019-01-11 | 中国农业科学院麻类研究所 | Herba Urticae triangularis rapid cuttage propagation method |
| CN107360958B (en) * | 2017-09-05 | 2020-05-26 | 中国农业科学院麻类研究所 | A method of rapid expansion of cannabis |
| CN109832099A (en) * | 2017-09-21 | 2019-06-04 | 云南谷益美农业开发有限公司 | A kind of industrial hemp fine breeding technology |
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| CN109452154B (en) * | 2018-11-09 | 2021-02-12 | 福建省中科生物股份有限公司 | Indoor cutting seedling method for hemp |
| CN110199735A (en) * | 2019-07-08 | 2019-09-06 | 厦门梓蔓生物科技有限公司 | A kind of industrial hemp numerous culture apparatus and breeding method fastly |
| CN110419397A (en) * | 2019-09-06 | 2019-11-08 | 黑龙江汉美生工业大麻科技有限公司 | A kind of Cultivate administration method improving No. 5 CBD contents of imperial hemp |
| CN110972918B (en) * | 2019-12-31 | 2021-09-28 | 云南省农业科学院经济作物研究所 | Water culture rooting method for cutting seedlings of medicinal industrial hemp |
| CN111837673A (en) * | 2020-07-02 | 2020-10-30 | 黑龙江润地汉麻科技有限公司 | Cuttage method for cannabis sativa in pharmaceutical industry |
| CN112438195A (en) * | 2020-11-18 | 2021-03-05 | 福建省中科生物股份有限公司 | Industrial hemp water culture cuttage method |
| CN112655475A (en) * | 2020-12-17 | 2021-04-16 | 黑龙江省科学院高技术研究院 | China hemp planting method |
| CN114788479A (en) * | 2022-04-24 | 2022-07-26 | 浙江省农业科学院 | Industrial hemp germplasm resource preservation and breeding method |
| CN115024166B (en) * | 2022-07-08 | 2023-09-08 | 云南大学 | Method for cultivating full female seedlings of hermaphrodite industrial cannabis sativa |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1887043A (en) * | 2006-07-13 | 2007-01-03 | 江苏省中国科学院植物研究所 | Fast propagation method of cannabis for inductrial use |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20090288226A1 (en) * | 2005-11-21 | 2009-11-19 | Hallahan David L | Method for manipulating growth, yield, and architecture in plants |
| CN1792144A (en) * | 2005-11-23 | 2006-06-28 | 卫德林 | Method for culturing mariajuana of bisexual type |
| GB2459125B (en) * | 2008-04-10 | 2013-01-02 | Gw Pharma Ltd | Method if extracting cannabichromene and its acid from Cannabis sativa plant material |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1887043A (en) * | 2006-07-13 | 2007-01-03 | 江苏省中国科学院植物研究所 | Fast propagation method of cannabis for inductrial use |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110839418A (en) * | 2019-11-28 | 2020-02-28 | 黑龙江雪域安康工业大麻科技有限责任公司 | Vegetative propagation method of industrial cannabis sativa L |
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