CN102911971A - Method for producing fuel ethanol by fermenting corncob processing residue - Google Patents
Method for producing fuel ethanol by fermenting corncob processing residue Download PDFInfo
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 86
- 239000000446 fuel Substances 0.000 title claims abstract description 7
- 238000004519 manufacturing process Methods 0.000 title abstract description 6
- 238000000855 fermentation Methods 0.000 claims abstract description 33
- 230000004151 fermentation Effects 0.000 claims abstract description 33
- 238000000034 method Methods 0.000 claims abstract description 30
- 238000002360 preparation method Methods 0.000 claims abstract description 29
- 230000007062 hydrolysis Effects 0.000 claims abstract description 26
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 26
- 150000001875 compounds Chemical class 0.000 claims abstract description 25
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 24
- 239000001913 cellulose Substances 0.000 claims abstract description 23
- 229920002678 cellulose Polymers 0.000 claims abstract description 23
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 15
- 102000004190 Enzymes Human genes 0.000 claims abstract description 14
- 108090000790 Enzymes Proteins 0.000 claims abstract description 14
- 108010047754 beta-Glucosidase Proteins 0.000 claims abstract description 6
- 102000006995 beta-Glucosidase Human genes 0.000 claims abstract description 6
- 240000008042 Zea mays Species 0.000 claims description 42
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 42
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 42
- 235000005822 corn Nutrition 0.000 claims description 42
- 239000010909 process residue Substances 0.000 claims description 41
- 235000019441 ethanol Nutrition 0.000 claims description 36
- 239000000463 material Substances 0.000 claims description 16
- 235000013379 molasses Nutrition 0.000 claims description 15
- 239000013543 active substance Substances 0.000 claims description 9
- 229910021645 metal ion Inorganic materials 0.000 claims description 9
- 238000006243 chemical reaction Methods 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 108010029541 Laccase Proteins 0.000 claims description 6
- 108010059820 Polygalacturonase Proteins 0.000 claims description 6
- 239000000835 fiber Substances 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 108091008146 restriction endonucleases Proteins 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 244000005700 microbiome Species 0.000 claims description 4
- 229920001221 xylan Polymers 0.000 claims description 4
- 150000004823 xylans Chemical class 0.000 claims description 4
- 230000002478 diastatic effect Effects 0.000 claims description 3
- 240000008892 Helianthus tuberosus Species 0.000 claims description 2
- 235000003230 Helianthus tuberosus Nutrition 0.000 claims description 2
- 229920002472 Starch Polymers 0.000 claims description 2
- 229920000136 polysorbate Polymers 0.000 claims description 2
- 230000035484 reaction time Effects 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- 239000002736 nonionic surfactant Substances 0.000 claims 1
- 239000002994 raw material Substances 0.000 abstract description 6
- 238000005265 energy consumption Methods 0.000 abstract description 4
- 238000007796 conventional method Methods 0.000 abstract 1
- 229960004756 ethanol Drugs 0.000 description 20
- 239000007788 liquid Substances 0.000 description 12
- 125000000969 xylosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)CO1)* 0.000 description 10
- 229940088598 enzyme Drugs 0.000 description 7
- HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical compound O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 description 7
- 108010059892 Cellulase Proteins 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 229940106157 cellulase Drugs 0.000 description 6
- 230000001360 synchronised effect Effects 0.000 description 5
- 235000013339 cereals Nutrition 0.000 description 4
- SRBFZHDQGSBBOR-LECHCGJUSA-N alpha-D-xylose Chemical compound O[C@@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-LECHCGJUSA-N 0.000 description 3
- 238000012262 fermentative production Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 229960003487 xylose Drugs 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 2
- 229920001214 Polysorbate 60 Polymers 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 230000001133 acceleration Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 241000228245 Aspergillus niger Species 0.000 description 1
- 235000016068 Berberis vulgaris Nutrition 0.000 description 1
- 241000335053 Beta vulgaris Species 0.000 description 1
- 101710099627 Beta-glucosidase 5 Proteins 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 244000138286 Sorghum saccharatum Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 230000000274 adsorptive effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000009229 glucose formation Effects 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 235000019991 rice wine Nutrition 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a method for producing fuel ethanol by fermenting corncob processing residue. The method comprises the steps of (1) preparing an obligate hydrolysis compound enzyme preparation for the corncob processing residue, wherein the compound enzyme preparation comprises endo cellulose, exo cellulose and beta-glucosidase; (2) adding the corncob processing residue to the obligate hydrolysis compound enzyme preparation for the corncob processing residue, wherein the content of the compound enzyme preparation is 0.05-0.5 g in per gram cellulose; pre-enzymolyzing for 0-36 h at a temperature of 45-60 DEG C; then adjusting the temperature to 24-44 DEG C; adding 0.002%-0.005% of saccharomyces cerevisiae; and performing simultaneous sacchrification and fermentation on the corncob processing residue to produce ethanol. Non-crop fermentation raw materials can be supplemented during the fermentation process. Compared with a conventional method, the method provided by the invention solves the problem of low final ethanol concentration of a fermentation unit during the process of producing ethanol by fermenting the corncob processing residue, and reduces energy consumption of an ethanol concentrating stage.
Description
Technical field
The present invention relates to a kind of production method of alcohol fuel, particularly take the method for corn cob process residues as the main raw material producing fuel ethyl alcohol by ferment.
Background technology
Produce a large amount of xylose residue and the furfural dregs of meeting generation in the product process such as furfural and wood sugar, general designation corn cob process residues take corn cob as Raw material processing.China's annual discharging furfuraldehyde waste slag nearly 3,000 ten thousand tons (CN 200910090506.5), the stacking of these waste residues is land occupation not only, and surrounding enviroment are polluted.After measured, the content of cellulose in the corn cob process residues butt accounts for more than 60%, hemicellulose about 8%, xylogen about 26%.CN00107969.7 discloses a kind of method of utilizing xylose residue to produce ethanol, this patent adopts first the aspergillus niger solid fermentation to produce CELLULASE, the CELLULASE of recycling preparation is mixed with xylose residue the Mierocrystalline cellulose in the xylose residue is hydrolyzed, then solid-liquid separation, obtain hydrolysis sugar liquid, glucose concn in the hydrolysis sugar liquid is 5%-12%, produce ethanol with adding saccharomycetes to make fermentation in the hydrolysis sugar liquid, according to the hydrolysis sugar concentration data of reporting in the patent, the accessible theoretical value of alcohol concn is 2.5 wt%-6wt% in the fermented liquid.CN200610131965.x discloses a kind of method of utilizing corn cob process residues fermentative production cellulosic ethanol, the method comprises utilizes the trichoderma pseudokiningii liquid submerged fermentation to produce cellulase, the anaerobism simultaneous saccharification and fermentation is produced ethanol, the steps such as ethanol purification, the alcohol concn in the final fermented liquid is 8% v/v.
Different from the conventional fibre ethanol production process, because the hemicellulose major part is hydrolyzed products such as generating wood sugar and furfural in the xylose residue, the wood sugar that the hydrolysis of corn cob process residues produces seldom, the glucose fermentation ethanol that only depends on cellulose hydrolysis to produce, the alcohol concn in the final fermented liquid is difficult to reach the level of grain alcohol (the fermented liquid alcohol concn can reach more than 12% v/v).Alcohol concn is low in the fermented liquid causes next step ethanol rectifying energy consumption excessive, has increased the alcohol production cost.
Summary of the invention
For the low problem of the final alcohol concn of fermentation unit in the existing corn cob process residues fermentative production of ethanol process, the present invention improves the alcohol concn in the xylose residue fermented liquid by composite, the means such as non-grain carbon source and simultaneous saccharification and fermentation of adding of cellulase, reduces the energy consumption in ethanol concentrate stage.
The present invention utilizes the method for corn cob process residues producing fuel ethyl alcohol by ferment, comprises following content:
(1) preparation corn cob process residues obligate hydrolysis compound enzymic preparation, contain Mierocrystalline cellulose restriction endonuclease, fiber excision enzyme and beta-glucosidase in the compound enzymic preparation, wherein, the concentration of Mierocrystalline cellulose restriction endonuclease is 5-30 g/L, the concentration of Mierocrystalline cellulose excision enzyme is 30-90 g/L, and the concentration of beta-glucosidase is 1-20 g/L;
(2) the corn cob process residues adds corn cob process residues obligate hydrolysis compound enzymic preparation, and the compound enzymic preparation consumption is 0.05-0.5 g/g Mierocrystalline cellulose; Then 45～60 ℃ of pre-enzymolysis 0-36 h adjust temperature to 24-44 ℃, add yeast saccharomyces cerevisiae 0.02 ‰-5 ‰ (take the dry yeast quality as benchmark), and corn cob process residues simultaneous saccharification and fermentation is produced ethanol;
(3) during the fermentation, preferably add the afterfermentation materials such as molasses, starch or jerusalem artichoke.
In the inventive method, in the corn cob process residues obligate hydrolysis compound enzymic preparation, can also contain one or more in xylan hydrolysis enzyme, polygalacturonase, laccase and the cofactor.The concentration of zytase is 0-20 g/L.The concentration of polygalacturonase is 0-5 g/L.Laccase concentration is 0-10 g/L.Cofactor comprises tensio-active agent and metal ion, and the concentration of tensio-active agent is 0.01-0.1 g/L, and the concentration of metal ion is 0.01-0.5 g/L.
In the inventive method, the tensio-active agent that cofactor in the corn cob process residues obligate hydrolysis compound enzymic preparation is used is preferably nonionogenic tenside, such as in the Tween series of surfactants one or more, be preferably one or more among Tween 80, Tween 60, Tween40, the Tween 20 etc.Metal ion can be selected Ca
2+, Cu
2+, Mn
2+, Zn
2+, Fe
2+, Mg
2+, Co
2+, K
+And Fe
3+In a kind of or any collocation use Ca
2+, Cu
2+, Mn
2+, Zn
2+, Fe
2+, Mg
2+, Co
2+, K
+And Fe
3+Concentration be respectively 0-0.04 g/L, 0-0.04 g/L, 0-0.02 g/L, 0-0.04 g/L, 0-0.04 g/L, 0-0.08 g/L, 0-0.05 g/L, 0-0.09 g/L, 0-0.1 g/L.Other material in the compound enzymic preparation is water.
In the inventive method, yeast saccharomyces cerevisiae can use any bacterial strain that can generate glucose fermentation ethanol, and the present invention produces ethanol in order to realize the high efficiency synchronous diastatic fermentation, preferred heatproof Saccharomyces Cerevisiae in S accharomyces cerevisiae FE-B.Saccharomyces cerevisiae FE-B is preserved on November 10th, 2008 that " China Committee for Culture Collection of Microorganisms's common micro-organisms " center " (be called for short CGMCC), its preserving number is CGMCC NO. 2735.Heatproof Saccharomyces Cerevisiae in S accharomyces cerevisiae FE-B is a strain heatproof yeast saccharomyces cerevisiae that filters out from rice wine yeast, can under 24 ~ 44 ℃ of conditions, grow, can at 24 ~ 44 ℃ of condition bottom fermentation glucose production ethanol, particularly can under comparatively high temps, realize synchronous saccharification and fermentation.
The simultaneous saccharification and fermentation condition of corn cob process residues is as follows: adopt the autoclave stirred pot to react, the mass ratio of corn cob process residues butt and water is 1:20-2:5 in the diastatic fermentation system; Add compound enzymic preparation, through behind the suitable pre-enzymolysis, carry out synchronous saccharification and fermentative production of ethanol under relatively high temperature, synchronous saccharification and fermenting process adopt intermittent stirring, and stir speed (S.S.) is specifically determined according to reactor volume and shape; The corn cob process residues adds mode can be according to denseness and the disposable adding of viscosity or the in batches adding of reaction system; Reaction times 48-144 h; PH is controlled at 4.0-7.0, preferred 4.5-5.0.
The time of the afterfermentation material of adding during the fermentation is preferably in fermentation carry out 5～40 hours after, the mode afterfermentation material that adds with stream.The fermented material that replenishes is preferably the Fermentable substrate matter in non-food grain source, most preferably be molasses, molasses can be that sugarcane or beet are the waste molasses that raw material production sucrose produces, also can be the molasses that sweet sorghum is squeezed the juice and produces after concentrated, all belong to non-grain raw material, total Sugar concentration is generally at 35%-60% in the molasses.Owing to contain glucose etc. has feedback inhibition to cellulosic hydrolysis in the xylose residue composition in the molasses.The replenishment method of afterfermentation material adopts flow velocity stream to add and enters into reaction system among the present invention, and the glucose concn in the reaction system is lower than 25 g/L.The add-on of afterfermentation material is counted the 20%-120 % of corn cob process residues butt quality with the total reducing sugar quality.
The enlarged culturing of yeast saccharomyces cerevisiae can adopt the ordinary method of this area, dilutes 25 times such as the molasses that will contain sugar 50%, adds the urea sterilization of 1 g/L, and access yeast ventilation stir culture 12 h get final product.
Though contain the more materials such as Mierocrystalline cellulose in the corn cob process residues, but because complex structure, be difficult for directly utilizing, the present invention is by preparation corn cob process residues obligate hydrolysis compound enzymic preparation, Mierocrystalline cellulose restriction endonuclease wherein, Mierocrystalline cellulose excision enzyme and beta-glucosidase synergy can effectively be hydrolyzed Mierocrystalline cellulose in the corn cob process residues.In corn cob process residues obligate hydrolysis compound enzymic preparation, further use the cofactors such as xylan hydrolysis enzyme, polygalacturonase, laccase, tensio-active agent and metal ion, can further improve the hydrolysis efficiency of the material such as Mierocrystalline cellulose in the corn cob process residues, ferment when can realize being hydrolyzed.Tensio-active agent can improve absorption solution and the adsorptive power between cellulose enzymatic hydrolysis system cellulase and the Mierocrystalline cellulose, improves cellulose hydrolysis efficiency; Metal ion mainly is used as the activator of various zymoproteins in the prozyme, improves the hydrolytic activity of zymoprotein.In addition, the inventive method reduces the energy consumption in ethanol concentrate stage by adding the alcohol concn in the means raising xylose residue fermented liquids such as non-grain carbon source and simultaneous saccharification and fermentation.
Description of drawings
Fig. 1 is the change curve of various material concentrations in the embodiment 1 reaction process system;
Fig. 2 is the change curve of various material concentrations in the embodiment 2 reaction process systems.
Embodiment
Further specify the solution of the present invention and effect below by embodiment, wherein not the percentage composition of clear and definite benchmark take weight as benchmark.
Use the composition of compound enzymic preparation among table 1 embodiment.
| | Embodiment | 1 | |
| The Mierocrystalline cellulose restriction endonuclease | 10 g/L | 15 g/L | |
| The fiber excision enzyme | 35 g/L | 40 g/L | |
| Beta-glucosidase | 5 g/L | 10 g/L | |
| The xylan hydrolysis enzyme | / | 12 g/L | |
| Polygalacturonase | / | 2 g/L | |
| Laccase | / | 6 g/L | |
| Tensio-active agent | Tween 80 0.05 g/L | Tween 60 0.05 g/L | |
| Metal ion | Mg 2+ 0.05 g/L；Cu 2+ 0.01 g/L | / |
Get 100 g corn cob process residues (moisture 75 %, fibre content is 63% in the corn cob process residues butt), add an amount of NaOH and regulate pH to 5.0, add 6.3 g compound enzymic preparations and 170 g water, place the 1L glass reaction pot, at 150 rpm, 50 ℃ of Water Under solutions.Add respectively 100 g corn cob process residues at 3 h, 5 h and 21 h.27.5 reducing temperature behind the h is 35 ℃, access cultured heatproof Saccharomyces Cerevisiae in S accharomyces cerevisiae FE-B, yeast access amount is 1 ‰ (ratios of dry yeast quality and system), 28 h begin that stream adds molasses (total sugar concentration 52.69% of molasses) in the system, the stream rate of acceleration is 3.7 g/h, it is 28h-60h that stream adds the time, adds 118.4 g molasses altogether in system.The alcohol concn in the xylose residue simultaneous saccharification and fermentation system is that 9.7% v/v(concentration by weight is 7.7 wt% behind 72 h as a result), see Fig. 1.
Utilize the 5L fermentor tank to carry out the corn cob process residues and molasses carry out common fermentation test.Get 1000 g corn cob process residues (moisture 75 %, fibre content is 63% in the corn cob process residues butt), add an amount of NaOH and regulate pH to 5.0, add 100 g compound enzymic preparations and 1700 g water, place 5 L fermentor tanks, access cultured heatproof Saccharomyces Cerevisiae in S accharomyces cerevisiae FE-B, yeast access amount is 1 wt ‰ (ratio of dry yeast quality and system), at 150 rpm, 42 ℃ of conditions are carried out synchronous saccharification and fermentation.16 h and 24 h add respectively 750 g and 250 g corn cob process residues raw materials at twice.0 h begins that stream adds molasses (total sugar concentration 52.69 %) in the system, and the stream rate of acceleration is 3.7 g/h, and it is 0 h-96h that stream adds the time, altogether adding 996 g molasses in the system.The alcohol concn in the xylose residue simultaneous saccharification and fermentation system is 10.27% v/v (concentration by weight is 8.1wt%) behind 115 h as a result, and residual sugar 2.16 wt % see Fig. 2.
Comparative example 1
According to the scheme of embodiment 1, just compound enzymic preparation is used common commercially available cellulase instead, and other is identical with embodiment 1, and the final alcohol concn of fermentation system is 6.5 wt%.
Comparative example 2
According to the scheme of embodiment 2, just yeast saccharomyces cerevisiae is common commercially available yeast saccharomyces cerevisiae, and other is identical with embodiment 2, and the final alcohol concn of fermentation system is 6.9 wt%.
Claims (10)
1. method of utilizing corn cob process residues producing fuel ethyl alcohol by ferment is characterized in that comprising following content:
(1) preparation corn cob process residues obligate hydrolysis compound enzymic preparation, contain Mierocrystalline cellulose restriction endonuclease, fiber excision enzyme and beta-glucosidase in the compound enzymic preparation, wherein, the concentration of Mierocrystalline cellulose restriction endonuclease is 5-30 g/L, the concentration of Mierocrystalline cellulose excision enzyme is 30-90 g/L, and the concentration of beta-glucosidase is 1-20 g/L;
(2) the corn cob process residues adds corn cob process residues obligate hydrolysis compound enzymic preparation, and the compound enzymic preparation consumption is 0.05-0.5 g/g Mierocrystalline cellulose; Then 45～60 ℃ of pre-enzymolysis 0-36 h adjust temperature to 24-44 ℃, take the dry yeast quality as benchmark, add yeast saccharomyces cerevisiae 0.02 ‰-5 ‰, and corn cob process residues simultaneous saccharification and fermentation is produced ethanol.
2. it is characterized in that in accordance with the method for claim 1: add during the fermentation molasses, starch or jerusalem artichoke afterfermentation material.
3. according to claim 1 or 2 described methods, it is characterized in that: in the corn cob process residues obligate hydrolysis compound enzymic preparation, also contain one or more in xylan hydrolysis enzyme, polygalacturonase, laccase and the cofactor, the concentration of zytase is 0-20 g/L, the concentration of polygalacturonase is 0-5 g/L, and laccase concentration is 0-10 g/L, and cofactor comprises tensio-active agent and metal ion, the concentration of tensio-active agent is 0.01-0.1 g/L, and the concentration of metal ion is 0.01-0.5 g/L.
4. in accordance with the method for claim 3, it is characterized in that: in the corn cob process residues obligate hydrolysis compound enzymic preparation, the tensio-active agent that cofactor is used is in the Tween series non-ionic surfactants one or more.
5. in accordance with the method for claim 3, it is characterized in that: in the corn cob process residues obligate hydrolysis compound enzymic preparation, Metal Ion Selective Electrode Ca
2+, Cu
2+, Mn
2+, Zn
2+, Fe
2+, Mg
2+, Co
2+, K
+And Fe
3+In a kind of or any collocation use Ca
2+, Cu
2+, Mn
2+, Zn
2+, Fe
2+, Mg
2+, Co
2+, K
+And Fe
3+Concentration be respectively 0-0.04 g/L, 0-0.04 g/L, 0-0.02 g/L, 0-0.04 g/L, 0-0.04 g/L, 0-0.08 g/L, 0-0.05 g/L, 0-0.09 g/L, 0-0.1 g/L.
6. in accordance with the method for claim 1, it is characterized in that: yeast saccharomyces cerevisiae uses heatproof Saccharomyces Cerevisiae in S accharomyces cerevisiae FE-B, Saccharomyces cerevisiae FE-B, be preserved on November 10th, 2008 that " China Committee for Culture Collection of Microorganisms's common micro-organisms " center " (be called for short CGMCC), its preserving number is CGMCC NO. 2735.
7. in accordance with the method for claim 1, it is characterized in that: fermenting process adopts the autoclave stirred pot to react, and the mass ratio of corn cob process residues butt and water is 1:20-2:5 in the diastatic fermentation system; The disposable adding of corn cob process residues or in batches adding; Reaction times 48-144 h; PH is controlled at 4.0-7.0.
8. in accordance with the method for claim 1, it is characterized in that: the time of the afterfermentation material of adding during the fermentation after fermentation is carried out 5～40 hours, the mode afterfermentation material that adds with stream.
9. it is characterized in that in accordance with the method for claim 2: the add-on of afterfermentation material is counted the 20%-120 % of corn cob process residues butt quality with the total reducing sugar quality.
10. according to claim 2 or 9 described methods, it is characterized in that: the replenishment method of afterfermentation material adopts flow velocity stream to add and enters into reaction system, and the glucose concn in the reaction system is lower than 25 g/L.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107354186A (en) * | 2017-07-27 | 2017-11-17 | 东华大学 | A kind of method that synchronous saccharification prepares bacteria cellulose |
| CN110753759A (en) * | 2017-04-20 | 2020-02-04 | 格拉特股份有限公司 | Method for simultaneously producing lactic acid and alcohol or biogas from grains |
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| CN1340627A (en) * | 2000-02-24 | 2002-03-20 | 能源环境和技术研究中心 | Method for producing ethanol from lignocellulose biomaterial by use of neu-heat-resistant enzyme |
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| CN110753759A (en) * | 2017-04-20 | 2020-02-04 | 格拉特股份有限公司 | Method for simultaneously producing lactic acid and alcohol or biogas from grains |
| CN110753759B (en) * | 2017-04-20 | 2023-09-19 | 富特罗股份有限公司 | Method for simultaneous production of lactic acid and alcohol or biogas from cereal |
| CN107354186A (en) * | 2017-07-27 | 2017-11-17 | 东华大学 | A kind of method that synchronous saccharification prepares bacteria cellulose |
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