CN102895323A - Method for extracting buckwheat flavonoid - Google Patents
Method for extracting buckwheat flavonoid Download PDFInfo
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- CN102895323A CN102895323A CN2012104223512A CN201210422351A CN102895323A CN 102895323 A CN102895323 A CN 102895323A CN 2012104223512 A CN2012104223512 A CN 2012104223512A CN 201210422351 A CN201210422351 A CN 201210422351A CN 102895323 A CN102895323 A CN 102895323A
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- Prior art keywords
- radix
- rhizoma fagopyri
- fagopyri tatarici
- extracting
- buckwheat
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- 229930003935 flavonoid Natural products 0.000 title abstract 4
- 150000002215 flavonoids Chemical class 0.000 title abstract 4
- 235000017173 flavonoids Nutrition 0.000 title abstract 4
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a method for extracting buckwheat flavonoid. The extracted buckwheat flavonoid has high purity and high extracting efficiency. The method comprises the following steps of: (1) adding one or more of tartary buckwheat husk, tartary buckwheat stalk and tartary buckwheat seed into an extractor, extracting with a propanol solution under the condition that the pressure is 0.3-0.4MPa and the temperature is 50-70 DEG C, and filtering an extracting solution; (2) evaporating and concentrating a filtrate under the condition that the pressure is 0.2-0.3MPa and the temperature is 60-70 DEG C; and (3) mixing the concentrated solution and water in the weight ratio of 1:(3-5), stirring, adding 2-methyl-butane serving as an organic solvent for extracting, extracting a lower suspension, centrifugally separating, and drying to obtain purified buckwheat flavonoid. The method has the advantages of high yield and high product purity.
Description
Technical field
The present invention relates to a kind of extracting method of Radix Et Rhizoma Fagopyri Tatarici flavone, the Radix Et Rhizoma Fagopyri Tatarici flavone purity of its extraction is high, and extraction efficiency is high.
Background technology
Radix Et Rhizoma Fagopyri Tatarici is the very few medicine-food two-purpose crop of occurring in nature, puts down in writing according to Compendium of Material Medica: the Radix Et Rhizoma Fagopyri Tatarici bitter in the mouth, and property is flat cold, the real the intestines and stomach of energy, physical strength profiting, continuous spirit, sharp knowledge, refining the five internal organs slag is dirty; All on the books to Radix Et Rhizoma Fagopyri Tatarici in Thousand Golden Prescriptions, " Chinese medicine voluminous dictionary " and pertinent literature: can calm the nerves, the swollen wind pain of lively atmosphere blood, the wide intestinal of sending down the abnormal ascending QI, heat clearing away, long-pendingization of dispelling are stagnant, bowel relieving, intestine moistening, relieving constipation, cough-relieving, relieving asthma, the effects such as antiinflammatory, antiallergic, heart tonifying, fat-reducing, beauty treatment
Radix Et Rhizoma Fagopyri Tatarici is Radix Et Rhizoma Fagopyri Tatarici, formal name used at school hull buckwheat (Tartar, pronunciation d á d á is the general designation to Chinese ancient northern minority name family), another name buckwheat leaf seven, Radix Rhapontici buckwheat, 10000 years buckwheats, spinach wheat, black wheat, flower buckwheat.Be that the nutritive value of Semen Fagopyri Esculenti exceeds much than sweet buckwheat.Particularly the content of bioflavonoids is 13.5 times of Semen Fagopyri Esculenti.
The unique activity composition of Radix Et Rhizoma Fagopyri Tatarici---Radix Et Rhizoma Fagopyri Tatarici flavone is a kind of complicated plyability material of varied organisms activity that has, Main Ingredients and Appearance has Quercetin, rutin, the luxuriant and rich with fragrance alcohol of bank, morin, wherein rutin accounts for 80%, and the ratio of this natural combination more has cooperative effect, easier being absorbed by the body.It has heat-clearing and toxic substances removing, blood circulation promoting and blood stasis dispelling, embolism resistance, improves microcirculation, can effectively reduce blood capillary fragility and permeability, prevention brain microvascular bleeding is arranged and safeguard the effect of eye circulation, four large physiologically actives:
1. blood circulation promoting and blood stasis dispelling, cholesterol reducing, Radix Et Rhizoma Fagopyri Tatarici flavone all has good therapeutical effect to coronary heart disease, cardiovascular and cerebrovascular vessel and peripheral angiopathy.Its blood circulation promoting and blood stasis dispelling, the microcirculatory effective percentage of improvement reach 88%.
2. sterilization, antibacterial, 0.08% Radix Et Rhizoma Fagopyri Tatarici solution can be killed escherichia coli, bacillus subtilis, staphylococcus aureus in 8 hours, and killing rate reaches 85%, can kill noxious bacteria in the body, can prevent to regulate the intestines and stomach inflammation.
3. antioxidation, anti-cancer, Radix Et Rhizoma Fagopyri Tatarici flavone can prevent the oils and fats autoxidation.Can press down in addition the activation of cell for cancer, prevent the hypertrophy of tumor, reach the effect of anti-cancer.
4. glucagon resistance factor, Radix Et Rhizoma Fagopyri Tatarici flavone can effectively resist " resistin ", improve cell, organize the sensitivity to insulin, strengthen the self-repairing capability of cell tissue, thereby reach the dual regulation to diabetes, high blood glucose is reduced, low blood sugar increasing, normal blood glucose is constant.Really reaching green, prevent and control diabetes and complication thereof safely, is natural glucokinin.
Existing Radix Et Rhizoma Fagopyri Tatarici flavone extracting method minutes three times mostly, the each extraction all about 4 hours extracted rear merge extractive liquid,, filters etc., exists on extraction time, efficient is low, the purity of the Radix Et Rhizoma Fagopyri Tatarici flavone of extraction is low, the problem that production cost is large.
Summary of the invention
It is high that the technical problem to be solved in the present invention provides a kind of yield, the extracting method of the Radix Et Rhizoma Fagopyri Tatarici flavone that product purity is large.
Content of the present invention is to comprise the steps:
(1) with in one or more adding extractors in KUQIAOPI, Radix Et Rhizoma Fagopyri Tatarici bar or the Radix Et Rhizoma Fagopyri Tatarici seed, be 0.3-0.4MPa with propanol solution at pressure, temperature is to extract under the 50-70 ℃ of condition, filters extracting solution;
(2) with filtrate at 0.2-0.3MPa, temperature is evaporation and concentration under the 60-70 ℃ of condition;
(3) concentrated solution and water are mixed according to the ratio of weight ratio 1: 3-5, stir, add organic solvent 2-methyl-butane and extract, take off a layer suspension, centrifugalize, drying gets the Radix Et Rhizoma Fagopyri Tatarici flavone of purification.
Extraction time in the described step (1) is 2-3 hour.
The evaporation and concentration time of described step (2) is 2-3 hour.
The number of times that described step (3) extracts is 3-5 time.
The addition of the middle organic solvent of described step (3) is 1-3 times of concentrated solution weight.
The invention has the beneficial effects as follows, raw material of the present invention come source range wide, can utilize Radix Et Rhizoma Fagopyri Tatarici skin, bar and seed these be rich in the raw material of Radix Et Rhizoma Fagopyri Tatarici flavone, by utilizing the organic solvent propanol to extract, filter evaporation and concentration, extract at last, separate, drying obtains Radix Et Rhizoma Fagopyri Tatarici flavone.Response time of whole process is short, the shortlyest can be 5 hours, and extraction efficiency is high, and the purity of the product of extraction is high, and purity 95% is so that the production cost of whole product reduces greatly.
The specific embodiment
Embodiment 1
The present invention includes following steps:
(1) KUQIAOPI, Radix Et Rhizoma Fagopyri Tatarici bar and Radix Et Rhizoma Fagopyri Tatarici seed being added in the extractor, is 0.3MPa with propanol solution at pressure, and temperature is to extract under 65 ℃ of conditions, and extraction time is 2 hours, filters extracting solution;
(2) with filtrate at 0.3MPa, temperature is evaporation and concentration under 70 ℃ of conditions, concentration time is 3 hours;
(3) concentrated solution and water are mixed according to the ratio of weight ratio 1:4, stir, the organic solvent 2-methyl-butane that adds 2 times of concentrated solution weight extracts, take off a layer suspension, again add organic solvent 2-methyl-butane and extract centrifugalize, drying gets the Radix Et Rhizoma Fagopyri Tatarici flavone of purification.Efficient liquid phase chromatographic analysis gets, and the purity of Radix Et Rhizoma Fagopyri Tatarici flavone is 98.2%.
Embodiment 2
The present invention includes following steps:
(1) KUQIAOPI and Radix Et Rhizoma Fagopyri Tatarici bar being added in the extractor, is 0.4MPa with propanol solution at pressure, and temperature is to extract under 65 ℃ of conditions, and extraction time is 3 hours, filters extracting solution;
(2) with filtrate at 0.2MPa, temperature is evaporation and concentration under 60 ℃ of conditions, concentration time is 3 hours;
(3) concentrated solution and water are mixed according to 1: 3 ratio of weight ratio, stir, the organic solvent 2-methyl-butane that adds 1 times of concentrated solution weight extracts, take off a layer suspension, again add organic solvent 2-methyl-butane and extract centrifugalize 4 times, drying gets the Radix Et Rhizoma Fagopyri Tatarici flavone of purification.Efficient liquid phase chromatographic analysis gets, and the purity of Radix Et Rhizoma Fagopyri Tatarici flavone is 97.1%.
Embodiment 3
The present invention includes following steps:
(1) the Radix Et Rhizoma Fagopyri Tatarici seed being added in the extractor, is 0.35MPa with propanol solution at pressure, and temperature is to extract under 70 ℃ of conditions, and extraction time is 2.5 hours, filters extracting solution;
(2) with filtrate at 0.3MPa, temperature is evaporation and concentration under 65 ℃ of conditions, concentration time is 2 hours;
(3) concentrated solution and water are mixed according to 1: 5 ratio of weight ratio, stir, the organic solvent 2-methyl-butane that adds 3 times of concentrated solution weight extracts, take off a layer suspension, again add organic solvent 2-methyl-butane and extract centrifugalize 2 times, drying gets the Radix Et Rhizoma Fagopyri Tatarici flavone of purification.Efficient liquid phase chromatographic analysis gets, and the purity of Radix Et Rhizoma Fagopyri Tatarici flavone is 96.5%.
Claims (5)
1. the extracting method of a Radix Et Rhizoma Fagopyri Tatarici flavone is characterized in that, comprises the steps:
(1) with in one or more adding extractors in KUQIAOPI, Radix Et Rhizoma Fagopyri Tatarici bar or the Radix Et Rhizoma Fagopyri Tatarici seed, be 0.3-0.4MPa with propanol solution at pressure, temperature is to extract under the 50-70 ℃ of condition, filters extracting solution;
(2) with filtrate at 0.2-0.3MPa, temperature is evaporation and concentration under the 60-70 ℃ of condition;
(3) concentrated solution and water are mixed according to the ratio of weight ratio 1: 3-5, stir, add organic solvent 2-methyl-butane and extract, take off a layer suspension, centrifugalize, drying gets the Radix Et Rhizoma Fagopyri Tatarici flavone of purification.
2. the extracting method of Radix Et Rhizoma Fagopyri Tatarici flavone according to claim 1 is characterized in that, the extraction time in the described step (1) is 2-3 hour.
3. the extracting method of Radix Et Rhizoma Fagopyri Tatarici flavone according to claim 1 is characterized in that, the evaporation and concentration time of described step (2) is 2-3 hour.
4. the extracting method of Radix Et Rhizoma Fagopyri Tatarici flavone according to claim 1 is characterized in that, the number of times that described step (3) extracts is 3-5 time.
5. the extracting method of Radix Et Rhizoma Fagopyri Tatarici flavone according to claim 1 is characterized in that, the addition of the middle organic solvent of described step (3) is 1-3 times of concentrated solution weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104223512A CN102895323A (en) | 2012-10-11 | 2012-10-11 | Method for extracting buckwheat flavonoid |
Applications Claiming Priority (1)
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| CN104435140A (en) * | 2014-11-07 | 2015-03-25 | 南京工业大学 | Ceramic membrane purification process of tartary buckwheat flavone extracting solution |
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| CN1965915A (en) * | 2006-11-11 | 2007-05-23 | 山西医科大学 | Method for extracting bitter buckwheat flavone in high purity |
| CN101066299A (en) * | 2007-06-13 | 2007-11-07 | 薛九荣 | Extracting process and product of tartary buckwheat extract with rich tartary buckwheat flavone |
| CN101972311A (en) * | 2010-11-02 | 2011-02-16 | 浙江大学 | Method for purifying Tartary buckwheat flavone |
| CN103040951A (en) * | 2012-09-07 | 2013-04-17 | 赵立地 | Method for extracting buckwheat flavonoids |
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| CN1965915A (en) * | 2006-11-11 | 2007-05-23 | 山西医科大学 | Method for extracting bitter buckwheat flavone in high purity |
| CN101066299A (en) * | 2007-06-13 | 2007-11-07 | 薛九荣 | Extracting process and product of tartary buckwheat extract with rich tartary buckwheat flavone |
| CN101972311A (en) * | 2010-11-02 | 2011-02-16 | 浙江大学 | Method for purifying Tartary buckwheat flavone |
| CN103040951A (en) * | 2012-09-07 | 2013-04-17 | 赵立地 | Method for extracting buckwheat flavonoids |
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| CN104435140A (en) * | 2014-11-07 | 2015-03-25 | 南京工业大学 | Ceramic membrane purification process of tartary buckwheat flavone extracting solution |
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