CN102885029B - Non-water leucorrhea specimen storing liquid and preparation method and pH value test collection tube - Google Patents
Non-water leucorrhea specimen storing liquid and preparation method and pH value test collection tube Download PDFInfo
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- CN102885029B CN102885029B CN2012104061236A CN201210406123A CN102885029B CN 102885029 B CN102885029 B CN 102885029B CN 2012104061236 A CN2012104061236 A CN 2012104061236A CN 201210406123 A CN201210406123 A CN 201210406123A CN 102885029 B CN102885029 B CN 102885029B
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Abstract
The invention discloses a non-water leucorrhea specimen storing liquid and a preparation method and pH value test collection tube of the liquid. The non-water leucorrhea specimen storing liquid comprises the following substances by weight percent: 96.5wt% of anhydrous glycerin, 0.2wt% of polyethyleneglycol 8000 as a surface active agent, 0.25wt% of bromocresol green, 0.05wt% of methyl red, 2.0wt% of absolute ethyl alcohol and 1.0wt% of mercapto sodium acetate. The non-water leucorrhea specimen storing liquid has the advantages that visible components such as bacteriums, cells, trichomonads and sick bodies of the leucorrhea can be protected by anhydrous glycerin (glycerol) when being frozen at a temperature less than -20 DEG C; the cell completeness of the visible components such as cells, bacteriums, trichomonads and the like can still be maintained after being redissolved without changing the morphology under a microscope; the leucorrhea can be solved in water in any concentration when needing to be diluted; mercapto sodium acetate is used as a reducing agent to prevent the specimen from inactivation caused by dehydration, oxidation and autolysis during the storage or transportation; and the non-water leucorrhea specimen storing liquid does not affect the cell lysis and determination of Chlamydia trachomatis or various causative agents of the leucorrhea.
Description
Technical field
The present invention relates to that leukorrhea in clinical medicine check gathers, research and the invention of pH test, the antivacuum collection container of freezing preservation, the pH value that relates in particular to the preparation method of a kind of non-water-white band specimen preserving liquid, this non-water-white band specimen preserving liquid and have this non-water-white band specimen preserving liquid is tested collection tube.
Background technology
In clinical medicine check now, leukorrhea routine inspection project comprises that microscopy, trichomonad, mould, bacterial vaginosis BV detect pH pH-value determination pH, the routine inspection of the vaginal fluids such as amine olfactory test.The inspection of trichomonad is generally biopsy, requires moisturizing, insulation censorship; The inspection requirements of mould, bacterial vaginosis BV (as the infection of Gardnerella) is preserved liquid without nutrition, prevents schizogamy; Vagina cleanness degree checks, requires to preserve cell complete, needs to wait to ooze moisturizing; Vaginal fluid pH pH-value determination pH, it is the at first project of the routine inspection of vaginal fluid, it is also the project that clinical meaning is arranged most, but generally adopt test paper method, in practical measurement, approximately there is 1/3 sample to go out xenogenesis variable color (white), normally should pH value<4.5 flavescence looks, it is blue that pH value>4.5 become, and this heterochromatic (white) can't judge the pH value.In addition, with the swab stick collection, deliver to inspection center nearly all dry, dilution (as physiological saline) is to dilute, and sample very little, as drips a physiological saline, has just all changed initial value pH value in sample.If adopt non-reviewer, by the clinical medical and nursing personnel, detected, human factor is large, and sample delays longer in clinical department.The collection preservation in transit of sample during now vaginal fluid conventional detection, the method for measuring of pH value, serious affect assay and clinical diagnosis.
Summary of the invention
In order to solve following technical problem: the preservation after vaginal fluid detection collection of specimens in the censorship process is in collection tube, to preserve the concentration of liquid dilution [H+], and affect the mensuration of the pH value of a small amount of sample of leukorrhea, the invention provides the preparation method of a kind of non-water-white band specimen preserving liquid, this non-water-white band specimen preserving liquid and have the pH value test collection tube of this non-water-white band specimen preserving liquid.
The present invention is achieved in that a kind of non-water-white band specimen preserving liquid, and it comprises the material of following percentage by weight: anhydrous glycerol, 96.5; As the PEG 8000 of surfactant, 0.2; Bromocresol green, 0.25; Methyl red, 0.05; Absolute ethyl alcohol, 2.0; Sodium thioglycolate, 1.0.
The invention still further relates to the preparation method of above-mentioned non-water-white band specimen preserving liquid, this preparation method comprises the steps: first bromocresol green, methyl red, sodium thioglycolate, PEG 8000, absolute ethyl alcohol to be mixed to the dissolving of milling, then add in anhydrous glycerol to mix and form this non-water-white band specimen preserving liquid, wherein, anhydrous glycerol: PEG 8000: bromocresol green: methyl red: absolute ethyl alcohol: the ratio of sodium thioglycolate is 96.5:0.2:0.25:0.05:2.0:1.0.
The invention still further relates to a kind of pH value test collection tube, it comprises test tube and is housed in invisible spectro non-water-white band specimen preserving liquid, and this non-water-white band specimen preserving liquid is above-mentioned non-water-white band specimen preserving liquid.
Further improvement as such scheme, this pH value test collection tube also comprises butyl rubber plug, safe Capsule and the label that is printed on colorimetric card, this butyl rubber plug jam-pack is preserved this non-water-white band specimen preserving liquid in the opening of this test tube for sealing, this safe Capsule is fixed on this test tube and is buckled in this butyl rubber plug outward, and this label is attached on this test tube lateral wall.
As the further improvement of such scheme, being housed in this invisible spectro this non-water-white band specimen preserving liquid is a fixed dosage.Preferably, this non-water-white band specimen preserving liquid is 50 μ L.
As the further improvement of such scheme, this test tube is the plastic cement test tube.
The preparation method of non-water-white band specimen preserving liquid of the present invention, this non-water-white band specimen preserving liquid and have the pH value test collection tube of this non-water-white band specimen preserving liquid, non-water-white band is wherein preserved liquid, for test pH value provides water-less environment, soda acid compound indicator bromocresol green and methyl red, non-water-white band specimen preserving liquid do not dilute the moisture content (namely not changing the concentration in reaction system of [H+]) of small sample; When the cotton swab that will speckle with leukorrhea, put into and preserve pipe (being test tube): cotton swab adsorbs the acid-base indicator colour developing of preserving liquid (being non-water-white band specimen preserving liquid) immediately, compares and reads the pH value with the colorimetric card on the label of preserving pipe; Preserving medium in liquid and be mainly anhydrous glycerol (glycerine) can protect below freezing-20 of leukorrhea visible component (bacterium, cell, trichomonad, sick body) ℃; After redissolution, still can keep the cell integrality of the visible components such as cell, bacterium, trichomonad, not change form under mirror, if while needing dilution, any concentration is water-soluble; Sodium thioglycolate is reductant, prevents that the dehydration oxidation oneself in sample preservation or transportation from decomposing and inactivation; Preserving liquid does not affect lysis and the mensuration of leukorrhea trachoma chlamydia antigen or multiple pathogens.
Embodiment
In order to make purpose of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
The non-water-white band specimen preserving liquid the present invention relates to comprises the material of following percentage by weight:
Anhydrous glycerol (glycerine) 96.5;
Surfactant (PEG8000) 0.2;
Bromocresol green 0.25;
Methyl red 0.05;
Absolute ethyl alcohol 2.0;
Sodium thioglycolate 1.0.
Non-water-white band specimen preserving liquid is when making, first bromocresol green, methyl red, sodium thioglycolate, PEG8000, absolute ethyl alcohol are mixed to the dissolving of milling, slowly add anhydrous glycerol (glycerine) to mix, be the non-water of leukorrhea and preserve liquid and contain pH pH-value determination pH mixed indicator, be called for short non-water-white band and preserve liquid.
Non-water-white band specimen preserving liquid in use, non-water-white band is preserved to liquid 50 μ L quantitatively to add in test tube (choosing in the present embodiment the plastic cement test tube), with butyl rubber plug jam-pack plastic cement test tube, buckle safe Capsule outward and prevent that butyl rubber plug is loosening owing to the foeign element such as rocking, label has been sticked on the lateral wall of plastic cement test tube, on label, be printed on Accurate pH value (3.0 to 6.4) colorimetric card, form like this and have the pH value test collection tube that this non-water-white band is preserved liquid.PH value test collection tube is applicable to the preservation of leukorrhea sample, freezing, transportation, pH pH-value determination pH, and rear visible component microscopy thaws.
The pH pH-value determination pH of small sample (0.01ml is following as the leucorrhea disease treatment sample).Require accurate high (being that the transition interval scope is little), the state plan birth management committee, in the pregnant front eugenic clinical detection project of organizing, leukorrhea pH value audit report, standard are that leukorrhea pH<4.5 are negative without clinical meaning,>4.5 positively have a clinical meaning.Methodology is defined as the pH test paper, in real work, see more heterochromatic reaction (normally should be by pH value<4.5 by the blue flavescence look of ash, the pH value is lower, yellow is darker.PH value>4.5 are by the blue virescence of ash, and the pH value is higher, and green is darker).And white can't be sentenced and comments the pH value, account for 30% left and right of measuring number of cases, the pH pH-value determination pH that solves this small sample of leukorrhea is a difficult problem in the clinical medicine check.
PH value test collection tube provided by the invention, non-water-white band is wherein preserved liquid, for test pH value provides water-less environment, soda acid compound indicator bromocresol green and methyl red, non-water-white band specimen preserving liquid do not dilute the moisture content (namely not changing the concentration in reaction system of [H+]) of small sample.When the cotton swab that will speckle with leukorrhea, put into and preserve pipe (being the plastic cement test tube): cotton swab adsorbs the acid-base indicator colour developing of preserving liquid (being non-water-white band specimen preserving liquid) immediately, compares and reads the pH value with the colorimetric card on the label of preserving pipe.Preserving medium in liquid and be mainly anhydrous glycerol (glycerine) can protect below freezing-20 of leukorrhea visible component (bacterium, cell, trichomonad, sick body) ℃; After redissolution, still can keep the cell integrality of the visible components such as cell, bacterium, trichomonad, not change form under mirror.If while needing dilution, any concentration is water-soluble.Sodium thioglycolate is reductant, prevents that the dehydration oxidation oneself in sample preservation or transportation from decomposing and inactivation.Preserving liquid does not affect lysis and the mensuration of leukorrhea trachoma chlamydia antigen or multiple pathogens.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (7)
1. a non-water-white band specimen preserving liquid, is characterized in that, it comprises the material of following percentage by weight: anhydrous glycerol, 96.5; As the PEG 8000 of surfactant, 0.2; Bromocresol green, 0.25; Methyl red, 0.05; Absolute ethyl alcohol, 2.0; Sodium thioglycolate, 1.0.
2. the preparation method of a non-water-white band specimen preserving liquid as claimed in claim 1, it is characterized in that, this preparation method comprises the steps: first bromocresol green, methyl red, sodium thioglycolate, PEG 8000, absolute ethyl alcohol to be mixed to the dissolving of milling, then add in anhydrous glycerol to mix and form this non-water-white band specimen preserving liquid, wherein, anhydrous glycerol: PEG 8000: bromocresol green: methyl red: absolute ethyl alcohol: the ratio of sodium thioglycolate is 96.5:0.2:0.25:0.05:2.0:1.0.
3. a pH value is tested collection tube, and it comprises test tube and is housed in invisible spectro non-water-white band specimen preserving liquid, it is characterized in that, this non-water-white band specimen preserving liquid is non-water-white band specimen preserving liquid claimed in claim 1.
4. pH value as claimed in claim 3 is tested collection tube, it is characterized in that, this pH value test collection tube also comprises butyl rubber plug, safe Capsule and the label that is printed on colorimetric card, this butyl rubber plug jam-pack is preserved this non-water-white band specimen preserving liquid in the opening of this test tube for sealing, this safe Capsule is fixed on this test tube and is buckled in this butyl rubber plug outward, and this label is attached on this test tube lateral wall.
5. pH value test collection tube as described as claim 3 or 4, is characterized in that, being housed in this invisible spectro this non-water-white band specimen preserving liquid is a fixed dosage.
6. pH value test collection tube as claimed in claim 5, is characterized in that, this non-water-white band specimen preserving liquid is 50 μ L.
7. pH value test collection tube as described as claim 3 or 4, is characterized in that, this test tube is the plastic cement test tube.
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| CN115466682B (en) * | 2022-11-14 | 2023-03-24 | 中国医学科学院北京协和医院 | Microbial preservation solution and using method thereof |
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| CN1793865A (en) * | 2005-12-22 | 2006-06-28 | 许国荣 | Combined detection reagent for leukorrhea-pH, amine test |
| KR101133997B1 (en) * | 2011-04-06 | 2012-04-05 | (주)셀트라존 | A composite for the use of cytological analysis |
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Address after: 231400 No. 2 Dongyibei Road, Tongcheng Economic Development Zone, Anqing City, Anhui Province Patentee after: Anhui Xinling Laboratory Medicine Technology Co., Ltd. Address before: 231400 Tongcheng Economic Development Zone, Anqing City, Anhui Province Patentee before: Anhui Sinic Laboratory Medicine Technology Co., Ltd. |
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