CN102879586A - Fluorescence immunoassay quantitative detection kit of microalbuminuria, and preparation method thereof - Google Patents
Fluorescence immunoassay quantitative detection kit of microalbuminuria, and preparation method thereof Download PDFInfo
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- CN102879586A CN102879586A CN2012103836444A CN201210383644A CN102879586A CN 102879586 A CN102879586 A CN 102879586A CN 2012103836444 A CN2012103836444 A CN 2012103836444A CN 201210383644 A CN201210383644 A CN 201210383644A CN 102879586 A CN102879586 A CN 102879586A
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Abstract
The invention relates to a fluorescence immunoassay quantitative detection kit of microalbuminuria, and a preparation method thereof. A sample pad, a combination pad, a nitrocellulose membrane and absorbent paper are pasted on the bottom liner of the quantitative detection kit in a successively lapped manner, wherein the combination pad is coated by fluorescent latex microsphere-marked human albumin antibodies; and the nitrocellulose membrane is coated by a detection line of albumin recombinant antigens and a quality control line of rabbit anti-mouse IgG antibodies. The fluorescence immunoassay quantitative detection kit can detect the content of microalbuminuria in human urine accurately and quantitatively, and has the characteristics of simple operation, high accuracy, high sensitivity, low cost, etc.
Description
Technical field
The invention belongs to medical immunology in-vitro diagnosis field, be specifically related to a kind of microalbumin fluorescence immunoassay immue quantitative detection reagent box and preparation method thereof.
Background technology
Under normal circumstances, when albumin is by the glomerular capillary net in the blood plasma, seldom enter crude urine, even pass through on a small quantity, 99% can be reuptaked by bent renal tubule, and albumin does not exist in therefore urinating eventually.But because the factors such as renal failure, diabetes and angiocardiopathy complication can cause the albumin pathologic to raise.Generally speaking, urine albumen obviously raises, and is common in serious Glomerular lesions, the slight expression tubular injury that increases.Albumin in the human urine reaches 30-300mg/ days or 20-200mg/L concentration and is called microalbumin (having another name called Microalbuminuria, Micoralbuminuria, MAU).Microalbumin is considered to one of important clinical signs such as renal failure, diabetes and angiocardiopathy complication usually.Therefore, there is the detection of level in albumin to early diagnosis, the early treatment of above-mentioned disease and reduces risk by important reference value and clinical meaning in the urine.
At present, the method for mensuration microalbumin has chip electrophoresis method, capillary electrophoresis, enzyme-linked immunosorbent assay, high performance liquid chromatography etc.Comparatively loaded down with trivial details in these detection methods, last long, expense is higher, is unfavorable for clinician's timely diagnosis and observation of curative effect.
Chinese patent application numbers 200810305085.9 discloses a kind of colloid selenium test paper for semi-quantitative determination of urinary trace albumin, and this test strips can detection level be not less than albumin in the 20 μ g/ml urines.Although this method is simple, accuracy is not high and can not quantitatively detect.Chinese patent application number 01126895.6 provides a kind of urinary albumin chip of fast detecting kidney damage, but it does not set forth key properties such as the preparation technology of chip, detection sensitivity, limits of identification, has limited the reliability of its application.Chinese patent application numbers 201110458518.6 discloses microscale urinary albumin colloidal gold detection kit and preparation technology thereof, has specifically described the preparation of this kit, and kit detection sensitivity 15 μ g/ml also have been described.But there is following defective in colloidal gold immunochromatographimethod:
⑴ colloid gold label process is the Electrostatic Absorption process, is a kind of physical process, thus in liquid phase less stable, often cause the protein molecular on the mark once more to come off;
⑵ only have when gold grain and gather when a certain amount of, and people's naked eyes just can be observed mauve band, and this color and background contrasts are little, thereby limited detection sensitivity.
Summary of the invention
The objective of the invention is the defective for above-mentioned prior art, provide a kind of highly sensitive, easy and simple to handle and can quantitatively detect fluorescence immunoassay quantitative measurement test strips of microalbumin and preparation method thereof.
The present invention solves the problems of the technologies described above the technical scheme of taking: a kind of microalbumin fluorescence immunoassay immue quantitative detection reagent box, sample pad, pad, nitrocellulose filter and thieving paper are pasted in overlap joint ground successively on end liner, be coated with the human albumin antibody of fluorescent latex microballoon mark on the described pad, be coated with the detection line of albumin recombinant antigen and the nature controlling line of rabbit anti-mouse igg antibody on the described nitrocellulose filter.
Described latex microsphere particle size range is 50nm-500nm, preferred 153nm.
Described fluorescent latex microballoon is to adsorb fluorescently-labeled Streptavidin by latex microsphere to prepare.
The fluorescence of described fluorescent latex microballoon is direct fluorescent material, a kind of among preferred fluorescein isothiocynate, RB 200, TRITC, fluorescein Cy5, the fluorescein Cy7.
Described pad material is glass fibre element film or polyester film.
A kind of preparation method of microalbumin fluorescence immunoassay immue quantitative detection reagent box comprises the steps:
1) preparation of coated fluorescent latex microballoon mark human albumin antibody pad:
With fluorescently-labeled Streptavidin and latex microsphere absorption combination, prepare the fluorescent latex microballoon;
Biotin is combined with the human albumin monoclonal antibody, prepares the biotinylation albumin antibody;
Gained fluorescent latex microballoon and biotinylation albumin antibody are mixed, namely get fluorescent latex microballoon mark human albumin antibody;
Gained fluorescent latex microballoon mark human albumin antibody is sprayed on the pad;
2) preparation of nitrocellulose filter:
Dilute respectively albumin recombinant antigen and rabbit anti-mouse igg antibody with coated damping fluid, and the albumin recombinant antigen after will diluting and rabbit anti-mouse igg antibody are sprayed on abreast respectively and form albumin detection line and rabbit anti-mouse igg antibody nature controlling line on the nitrocellulose filter;
3) on end liner, mutually paste sample pad, pad, nitrocellulose filter and thieving paper in turn obtain test paper plate to overlap joint, cut into as requested the test strips of proper width.
The present invention can detect microalbumin content in the human urine by accurate quantitative analysis, has the characteristics such as easy and simple to handle, that accuracy is high, highly sensitive, cost is low.
Description of drawings
Fig. 1 is microalbumin fluorescence immunoassay immue quantitative detection reagent box structural representation of the present invention.
Fig. 2 is the typical curve of the prepared kit of the embodiment of the invention 1.
Fig. 3 is the range of linearity correlativity of the prepared kit of the embodiment of the invention 1.
Fig. 4 be the prepared kit of the embodiment of the invention 1 with the microalbumin kit testing result correlativity of producing with Olympus AU5400 full automatic biochemical apparatus and supporting Britain Landau relatively.
Wherein, Fig. 1: 1, end liner; 2, sample pad; 3, pad; 4, nitrocellulose filter; 5, thieving paper; 6, detection line; 7, nature controlling line.
Embodiment
The present invention is further detailed explanation below in conjunction with the drawings and specific embodiments.
Microalbumin fluorescence immunoassay immue quantitative detection reagent box, on end liner 1, overlap successively ground sample pad 2, pad 3, nitrocellulose filter 4 and thieving paper 5, be coated with the human albumin antibody of fluorescent latex microballoon mark on the described pad 3, the detection line 6 of coated albumin recombinant antigen and the nature controlling line 7 of rabbit anti-mouse igg antibody are arranged on the described nitrocellulose filter 4.
Further described latex microsphere particle size range is 50nm-500nm, preferred 153nm.
Described fluorescent latex microballoon is to adsorb fluorescently-labeled Streptavidin by latex microsphere to prepare.
Described fluorescence is direct fluorescent material, a kind of among preferred fluorescein isothiocynate, RB 200, TRITC, fluorescein Cy5, the fluorescein Cy7.
Embodiment 1 microalbumin fluorescence immunoassay immue quantitative detection reagent box preparation method is as follows:
1) preparation of fluorescent latex microballoon
The preparation of fluorescent latex microballoon: with adsorption-buffering liquid (citrate buffer of 50mM, pH5.8) dilution particle diameter be the 153nm latex microsphere to final concentration 20mg/ml, volume is 5ml, makes latex microsphere suspending liquid; Add an amount of fluorescein Cy5 labelled streptavidin (grinding brilliant bio tech ltd available from Shanghai) in adsorption-buffering liquid, final volume is 5ml; Above-mentioned latex microsphere suspending liquid is joined in the above-mentioned adsorption-buffering liquid that contains fluorescein-labelled Streptavidin, make mixed liquor; With at room temperature temperature bath of gained mixed liquor 1-2 hour, and constantly stir, then centrifugal, collecting precipitation, precipitation is placed 4 ℃ of preservations with store buffer liquid (containing 0.05% caseic adsorption-buffering liquid) dissolving, and is for subsequent use.
2) preparation of biotinylation albumin antibody
Human albumin monoclonal anti body and function 0.1mol/L, pH8.0 sodium bicarbonate buffer liquid are diluted to 1mg/ml, with 0.1mol/L, pH8.0 sodium bicarbonate buffer liquid the human albumin monoclonal antibody are fully dialysed alternately; NHSB with 1ml DMSO dissolving 1mg obtains NHSB solution; Add 20 μ l NHSB solution to above-mentioned 1ml albumin monoclonal antibody solution, stirring at room 2-4 hour, continued stirring at room 10 minutes, then with 20mM, the dialysis of pH7.2 PBS damping fluid, namely get biotinylation albumin monoclonal antibody.
3) preparation of fluorescent latex microballoon mark human albumin antibody
With step 1) the fluorescent latex microballoon and the step 2 that make) the biotinylation albumin monoclonal antibody that makes mixes, and reacts centrifugal after 30 minutes, and sediment returns to original volume with the dissolving of store buffer liquid.
4) preparation of coated fluorescent latex microballoon mark human albumin antibody pad 3
With the fluorescent latex microballoon mark human albumin monoclonal antibody that step 3) makes, press 3.0 μ l/cm
3Consumption be sprayed on the glass fibre element film 3.
5) preparation of nitrocellulose filter 4
A) preparation of coated damping fluid: with the PBS of 0.025M, pH7.4, with 0.22 μ membrane filtration, place 4 ℃ for subsequent use, the term of validity 7 days.
B) preparation of confining liquid: will contain 1%BSA, 1% sucrose, the PBS of 0.025M, pH7.5, with 0.22 μ membrane filtration, place 4 ℃ for subsequent use, the term of validity 3 days.
C) preparation of albumin recombinant antigen detection line 6: the albumin recombinant antigen is pressed the concentration of 2.0mg/ml, peristaltic pump is awarded liquid measure 0.4ml/min, line speed 50m/20min, and 20 ℃ of forced air dryings are 12 hours in drying box.
D) preparation of nature controlling line 7: the concentration of rabbit anti-mouse igg antibody being pressed 8mg/ml, peristaltic pump is awarded liquid measure 0.4ml/min, and line speed 50m/20min is in nitrocellulose filter 5 line, this line is parallel with detection line 6, puts into 20 ℃ of forced air dryings of drying box 12 hours.
The nitrocellulose filter 4 that e) will contain detection line 6 and nature controlling line 7 with above-mentioned confining liquid took out rearmounted 37 ℃ of lower drying and processings two hours in 37 ℃ of sealings 60 minutes, and envelope is for subsequent use.
6) test strips assembling
On end liner 1, mutually paste in turn sample pad 2, glass fibre element film 3, nitrocellulose filter 4 and thieving paper 5 overlap joint and obtain test paper plate, cut into as requested the test strips of proper width.
1) preparation of fluorescent latex microballoon
The preparation of fluorescent latex microballoon: with adsorption-buffering liquid (citrate buffer of 50mM, pH5.8) dilution particle diameter be the 500nm latex microsphere to final concentration 30mg/ml, volume is 6ml, makes latex microsphere suspending liquid; In adsorption-buffering liquid, final volume is 6ml to add an amount of red fluorescence element rhodamine labelled streptavidin (available from Shanghai enzyme connection bio tech ltd); Above-mentioned latex microsphere suspending liquid is joined in the above-mentioned adsorption-buffering liquid that contains red fluorescence element rhodamine labelled streptavidin, make mixed liquor; With at room temperature temperature bath of gained mixed liquor 1-2 hour, and constantly stir, then centrifugal, collecting precipitation, precipitation is placed 4 ℃ of preservations with store buffer liquid (containing 0.06% caseic adsorption-buffering liquid) dissolving, and is for subsequent use.
2) preparation of biotinylation albumin antibody (with the preparation of the biotinylation albumin antibody among the embodiment 1)
3) preparation of fluorescent latex microballoon mark human albumin antibody (with the preparation of the fluorescent latex microballoon mark human albumin antibody among the embodiment 1)
4) preparation of coating fluorescent latex microballoon mark human albumin antibody pad 3
With the fluorescent latex microballoon mark human albumin monoclonal antibody that step 3) makes, press 2.0 μ l/cm
3Consumption be sprayed on the polyester film 3.
5) preparation of nitrocellulose filter 4 (with the preparation of the nitrocellulose filter 4 among the embodiment 1)
6) test strips assembling (with test strips assembling among the embodiment 1)
1) drawing standard curve
Add variable concentrations albumin antigen standard items in the microalbumin fluorescence immunoassay quantitative measurement test strips sample pad for preparing by embodiment 1 and (get six variable concentrations, be respectively 10,20,40,80,160,200mg/L, each concentration is established 3 repetitions), fluorescence immunoassay quantitative analysis instrument Getein1100 by Nanjing base egg bio tech ltd reads detection line 7 and nature controlling line 8 signals, experimental result and analysis in table 1.
Table 1 albumin standard items testing result
With the signal averaging drawing standard curve of microalbumin antigen standard items concentration and mensuration, as shown in Figure 2.
2) detect the range of linearity
Adopt the test strips of the embodiment of the invention 1 preparation, do and detect range of linearity experiment.Get the microalbumin standard items, be diluted to 8 concentration with 1% casein damping fluid, its concentration range is 10mg/L-200mg/L, each concentration replication 3 times, mean value and the theoretical concentration of measuring concentration are carried out linear regression analysis, calculate regression equation y=1.003x+0.491, correlation coefficient r=0.9994 shows test strips of the present invention correlativity in the 10mg/L-200mg/L range of linearity fine (seeing accompanying drawing 3).
3) sensitivity
Take 1% casein as dummy, test strips with the embodiment of the invention 1 preparation is measured, repeat 20 times, calculating reading average V is 2381.20, standard deviation SD is 19.62, calculating fluorescence immunoassay quantitative analysis instrument Getein1100 mensuration reading variable quantity calculating V+2SD as 2420.449 according to add twice standard deviation method for reporting take blank average, is 1.71mg/L according to regression equation calculation dummy concentration.
With concentration after the dilution be respectively 8mg/L, 10mg/L, 12mg/L, 14mg/L, 20mg/L albumin standard solution is measured fluorescence immunoassay quantitative analysis instrument Getein1100 replication 10 times, calculating respectively coefficient of variation CV is 19.61%, 9.46%, 8.44%, 7.52%, 8.46%.This shows, least concentration level 10 mg/L are the Reportable range lower limit.
4) repeatability and accuracy
The microalbumin standard solution of preparation 20mg/L and 100mg/L adopts the test strips of the embodiment of the invention 1 preparation to measure, and each concentration difference replication 5 times calculates respectively and measures average and standard deviation.Calculate the coefficient of variation and carry out the repeatability investigation, the result shows that the coefficient of variation is respectively 0.05 and 0.06; Calculate relative deviation and carry out the accuracy investigation, relative deviation is respectively 0.60% and 5.30%.
Table 2 repeatability and accuracy experiment
| Sequence number | Standard value 20mg/L | Standard value 100mg/L |
| 1 | 21.7 | 114.8 |
| 2 | 20.4 | 105.7 |
| 3 | 19.5 | 104.2 |
| 4 | 19.8 | 96.3 |
| 5 | 19.2 | 105.5 |
| Mean value | 20.12 | 105.3 |
| Standard deviation | 0.99 | 6.57 |
| The coefficient of variation (%) | 4.91% | 6.24% |
| Relative deviation (%) | 0.60% | 5.30% |
3.5 carry out clinical performance and validity comparative analysis with the microalbumin kit of Olympus AU5400 full automatic biochemical apparatus and the Landau production of supporting Britain
Adopt the test strips of the embodiment of the invention 1 preparation to measure, the urine five equilibrium that will contain microalbumin, get the sample pipetting volume of 100 μ l to the sample pad 2 of test strips, the fluorescence immunoassay quantitative analysis instrument Getein1100 by Nanjing base egg bio tech ltd reads detection line 7 and nature controlling line 8 signals.From same aliquot sample, adopt the microalbumin kit of comparison system Olympus AU5400 full automatic biochemical apparatus and the Landau production of supporting Britain to carry out clinical performance and validity comparative analysis.Adopt this mode to prepare 200 duplicate samples and adopt two detection systems, Fig. 4 has shown the measured value of two systems, the fine r=0.984 of its correlativity, and P＞0.05, medical science determines level place relative deviation less than 10%, the result meets the clinical analysis requirement.Therefore, no difference of science of statistics between two kinds of methods.
Claims (8)
1. microalbumin fluorescence immunoassay immue quantitative detection reagent box, sample pad (2), pad (3), nitrocellulose filter (4) and thieving paper (5) are pasted in overlap joint ground successively on end liner (1), it is characterized in that being coated with on the described pad (3) the human albumin antibody of fluorescent latex microballoon mark, be coated with the detection line (6) of albumin recombinant antigen and the nature controlling line (7) of rabbit anti-mouse igg antibody on the described nitrocellulose filter (4).
2. described microalbumin fluorescence immunoassay immue quantitative detection reagent box according to claim 1 is characterized in that described latex microsphere particle size range is 50nm-500nm.
3. described microalbumin fluorescence immunoassay immue quantitative detection reagent box according to claim 2 is characterized in that described latex microsphere particle diameter is 153nm.
4. described microalbumin fluorescence immunoassay immue quantitative detection reagent box according to claim 1 is characterized in that described fluorescent latex microballoon is to adsorb fluorescently-labeled Streptavidin by latex microsphere to prepare.
5. microalbumin fluorescence immunoassay immue quantitative detection reagent box according to claim 1, the fluorescence that it is characterized in that described fluorescent latex microballoon is direct fluorescent material.
6. described microalbumin fluorescence immunoassay immue quantitative detection reagent box according to claim 5 is characterized in that described direct fluorescent material is a kind of among fluorescein isothiocynate, RB 200, TRITC, fluorescein Cy5, the fluorescein Cy7.
7. microalbumin fluorescence immunoassay immue quantitative detection reagent box according to claim 1 is characterized in that described pad (3) material is glass fibre element film or polyester film.
8. an a kind of preparation method of microalbumin fluorescence immunoassay immue quantitative detection reagent box as claimed in claim 1 comprises the steps:
1) preparation of coated fluorescent latex microballoon mark human albumin antibody pad:
With fluorescently-labeled Streptavidin and latex microsphere absorption combination, prepare the fluorescent latex microballoon;
Biotin is combined with the human albumin monoclonal antibody, prepares the biotinylation albumin antibody;
Gained fluorescent latex microballoon and biotinylation albumin antibody are mixed, namely get fluorescent latex microballoon mark human albumin antibody;
Gained fluorescent latex microballoon mark human albumin antibody is sprayed on the pad;
2) preparation of nitrocellulose filter:
Dilute respectively albumin recombinant antigen and rabbit anti-mouse igg antibody with coated damping fluid, and the albumin recombinant antigen after will diluting and rabbit anti-mouse igg antibody are sprayed on abreast respectively and form albumin detection line and rabbit anti-mouse igg antibody nature controlling line on the nitrocellulose filter;
3) on end liner, mutually paste sample pad, pad, nitrocellulose filter and thieving paper in turn obtain test paper plate to overlap joint, cut into as requested the test strips of proper width.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104345148A (en) * | 2013-07-28 | 2015-02-11 | 嘉兴朝云帆生物科技有限公司 | Test strip and method for detecting protein by using immunochromatography |
| CN105759050A (en) * | 2016-01-29 | 2016-07-13 | 苏州联辰生物技术有限公司 | Immunofluorescence kit for quantitatively detecting content of troponin I and preparation method |
| CN106645745A (en) * | 2016-10-19 | 2017-05-10 | 山东大学齐鲁医院 | Homogenous-phase fluorescent immune reagent for rapidly and quantitatively detecting trace albumin, and preparation and detection method thereof |
| CN106872420A (en) * | 2016-12-27 | 2017-06-20 | 厦门奥德生物科技有限公司 | The kit and method of a kind of time-resolved fluorescence quantitative determination microdose urine protein |
| CN109613256A (en) * | 2018-11-21 | 2019-04-12 | 杭州康知生物科技有限公司 | It urinates four kinds of traces of albumin and quantifies joint inspection fluorescence immune chromatography kit and preparation method |
| CN112526142A (en) * | 2020-11-25 | 2021-03-19 | 杭州福德敏生物技术有限公司 | Egg immunofluorescence detection test strip and application and detection method thereof |
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| CN112595847A (en) * | 2020-12-31 | 2021-04-02 | 杭州福德敏生物技术有限公司 | Shrimp immunofluorescence detection test strip and application |
| CN112858682A (en) * | 2019-11-27 | 2021-05-28 | 菲鹏生物股份有限公司 | Test strip for detecting microalbumin in urine, preparation method thereof, detection kit and detection method |
| CN112876562A (en) * | 2019-11-30 | 2021-06-01 | 江苏众红生物工程创药研究院有限公司 | Anti-human serum albumin antibody and application thereof |
| CN113848326A (en) * | 2021-09-09 | 2021-12-28 | 派恩特(中山)生物科技有限公司 | Urine microalbumin detection kit and preparation thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003042696A1 (en) * | 2001-11-14 | 2003-05-22 | Prion Developmental Laboratories, Inc. | Rapid prion-detection device, system, and test kit |
| CN101493460A (en) * | 2009-02-25 | 2009-07-29 | 江西中德生物工程有限公司 | Method for producing fluorescent microballoons immune chromatography test paper stripe and quantitative determination method |
| EP1664783B1 (en) * | 2003-09-23 | 2011-05-11 | Epinex Diagnostic, Inc. | Rapid test for glycated albumin |
| CN102539786A (en) * | 2011-12-31 | 2012-07-04 | 上海凯创生物技术有限公司 | Microscale urinary albumin colloidal gold detection kit and preparation technology thereof |
| CN102680704A (en) * | 2012-04-28 | 2012-09-19 | 广州鸿琪光学仪器科技有限公司 | immunofluorescence test strip module for rapidly and quantitatively testing microalbumin, test card module made of same and preparation method thereof |
| CN102692504A (en) * | 2012-06-26 | 2012-09-26 | 南京基蛋生物科技有限公司 | D-dimer quantitative fluorescence immunoassay test strip and preparation method thereof |
| CN102707067A (en) * | 2012-05-24 | 2012-10-03 | 蓝十字生物药业（北京）有限公司 | Test strip for semi-quantitative detection of microalbuminuria |
-
2012
- 2012-10-11 CN CN2012103836444A patent/CN102879586A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003042696A1 (en) * | 2001-11-14 | 2003-05-22 | Prion Developmental Laboratories, Inc. | Rapid prion-detection device, system, and test kit |
| EP1664783B1 (en) * | 2003-09-23 | 2011-05-11 | Epinex Diagnostic, Inc. | Rapid test for glycated albumin |
| CN101493460A (en) * | 2009-02-25 | 2009-07-29 | 江西中德生物工程有限公司 | Method for producing fluorescent microballoons immune chromatography test paper stripe and quantitative determination method |
| CN102539786A (en) * | 2011-12-31 | 2012-07-04 | 上海凯创生物技术有限公司 | Microscale urinary albumin colloidal gold detection kit and preparation technology thereof |
| CN102680704A (en) * | 2012-04-28 | 2012-09-19 | 广州鸿琪光学仪器科技有限公司 | immunofluorescence test strip module for rapidly and quantitatively testing microalbumin, test card module made of same and preparation method thereof |
| CN102707067A (en) * | 2012-05-24 | 2012-10-03 | 蓝十字生物药业（北京）有限公司 | Test strip for semi-quantitative detection of microalbuminuria |
| CN102692504A (en) * | 2012-06-26 | 2012-09-26 | 南京基蛋生物科技有限公司 | D-dimer quantitative fluorescence immunoassay test strip and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 李莉等: "抗人血白蛋白单克隆抗体的制备及其免疫金标试纸条的研制", 《中国卫生检验杂志》 * |
Cited By (14)
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| CN104345148A (en) * | 2013-07-28 | 2015-02-11 | 嘉兴朝云帆生物科技有限公司 | Test strip and method for detecting protein by using immunochromatography |
| CN105759050A (en) * | 2016-01-29 | 2016-07-13 | 苏州联辰生物技术有限公司 | Immunofluorescence kit for quantitatively detecting content of troponin I and preparation method |
| CN106645745A (en) * | 2016-10-19 | 2017-05-10 | 山东大学齐鲁医院 | Homogenous-phase fluorescent immune reagent for rapidly and quantitatively detecting trace albumin, and preparation and detection method thereof |
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| CN106872420B (en) * | 2016-12-27 | 2019-12-17 | 厦门奥德生物科技有限公司 | Kit and method for time-resolved fluorescence quantitative detection of microalbuminuria |
| CN109613256A (en) * | 2018-11-21 | 2019-04-12 | 杭州康知生物科技有限公司 | It urinates four kinds of traces of albumin and quantifies joint inspection fluorescence immune chromatography kit and preparation method |
| CN112858682A (en) * | 2019-11-27 | 2021-05-28 | 菲鹏生物股份有限公司 | Test strip for detecting microalbumin in urine, preparation method thereof, detection kit and detection method |
| CN112876562A (en) * | 2019-11-30 | 2021-06-01 | 江苏众红生物工程创药研究院有限公司 | Anti-human serum albumin antibody and application thereof |
| CN112876562B (en) * | 2019-11-30 | 2022-05-27 | 江苏众红生物工程创药研究院有限公司 | Anti-human serum albumin antibody and application thereof |
| CN112526124A (en) * | 2020-11-25 | 2021-03-19 | 杭州福德敏生物技术有限公司 | Peanut immunofluorescence detection test strip and application and detection method thereof |
| CN112526142A (en) * | 2020-11-25 | 2021-03-19 | 杭州福德敏生物技术有限公司 | Egg immunofluorescence detection test strip and application and detection method thereof |
| US20220163523A1 (en) * | 2020-11-25 | 2022-05-26 | Hangzhou Fudemin Biotechnology Co., Ltd. | Test strip for peanut immunofluorescence assay (ifa), use thereof and detection method |
| CN112595847A (en) * | 2020-12-31 | 2021-04-02 | 杭州福德敏生物技术有限公司 | Shrimp immunofluorescence detection test strip and application |
| CN113848326A (en) * | 2021-09-09 | 2021-12-28 | 派恩特(中山)生物科技有限公司 | Urine microalbumin detection kit and preparation thereof |
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Address after: Liuhe District of Nanjing City, Jiangsu province 211505 Yangtze River Industrial Development Zone Bo Fu Road No. 9 Applicant after: Ji Dan biotech inc Address before: Liuhe District of Nanjing City, Jiangsu province 211505 Yangtze River Industrial Development Zone Bo Fu Road No. 9 Applicant before: Nanjing Egg-based Biotechnology Co., Ltd. |
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Application publication date: 20130116 |