CN102871125A - Bone calcium extraction method capable of reducing fat content, and osteocalcin - Google Patents
Bone calcium extraction method capable of reducing fat content, and osteocalcin Download PDFInfo
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- CN102871125A CN102871125A CN2012103824324A CN201210382432A CN102871125A CN 102871125 A CN102871125 A CN 102871125A CN 2012103824324 A CN2012103824324 A CN 2012103824324A CN 201210382432 A CN201210382432 A CN 201210382432A CN 102871125 A CN102871125 A CN 102871125A
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- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 title claims abstract description 34
- 239000011575 calcium Substances 0.000 title claims abstract description 34
- 229910052791 calcium Inorganic materials 0.000 title claims abstract description 34
- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 32
- 238000000605 extraction Methods 0.000 title claims abstract description 5
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- 108090000573 Osteocalcin Proteins 0.000 title abstract 2
- 230000007062 hydrolysis Effects 0.000 claims abstract description 26
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 24
- 238000002360 preparation method Methods 0.000 claims abstract 3
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- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The invention discloses a bone calcium extraction method capable of reducing fat content, and osteocalcin prepared by the method. The method comprises the following steps of three times of derosination, twice centrifugation and a two-stage protease hydrolysis. Fat in bone powder prepared by the derosination process of the invention is completely removed basically, allowing the fat content in the calcium preparation obtained by enzymatic hydrolysis to be lower than 0.1%. The product is safer and healthier for consumers with cardiovascular diseases, but does not increase economic burden.
Description
Technical field
The present invention relates to bone calcium extraction process, belong to food processing field.
Background technology
Along with the aging of society, in western countries, the illness rate of osteoporosis occupies first of metabolic bone disease.The misery that the osteoporosis illness brings patient and family is self-evident.According to statistics, China has approximately at present and surpasses 100,000,000 patients with osteoporosis, expects the year two thousand fifty will be increased to 200,000,000 1 thousand ten thousand people.Cause the factor of increasing people's calcium deficiency: live and work rhythm is accelerated, operating pressure is large and live irregular, the structure that is not careful in one's diet reasonably combined, calcium was taken in and was less than 600 milligrams of persons and can thinks the calcium insufficiency of intake every day, easily caused the shortage of calcium in the body; Some a middle-aged person's work strains lack self health consciousness, think little of outdoor activity, and proper interior synthetic vitamin D is reduced, and affect absorption and the utilization of calcium; Nutrition is taken in unbalanced or is lacked the necessary nutrient of human body.
The calcium agent of existing market mostly is the synthetic calcium of industry.The calcium agent nutritional labeling that industry is synthesized is single, is difficult to satisfy many-sided nutritional need in the bone growth metabolic process, and effect of supplemented calcium is undesirable.Animal Bone is rich in the several kinds of mineral elements such as calcium, phosphorus, iron, magnesium, and with people's bone photo seemingly, the skeleton cell has stronger affinity to homologue's cell, thereby utilization rate is higher.
Chinese patent CN1087793A and US Patent No. 6342252B1 disclose a kind of technique of utilizing enzyme solution to extract the calcium element from ox bone.Enzymolysis bone calcium has suitable, nutritious, the calcareous absorption rate high of calcium phosphorus ration.From the market sale situation, enzymolysis bone calcium is subjected to liking of consumers in general deeply, takes the osteoporotic successful of rear improvement, has obtained significant economic benefit.
But in the bone calcium that above-mentioned technique makes, degreasing method mainly is to implement in the boiling mode, is difficult to remove the fat of high-load in the sclerotin, causes final product fat content higher.For the consumer with angiocardiopathy, the hazards that too high fat intake obviously can raise and fall ill.Therefore, from animal skeleton, extract in the process of calcium element, remove excess fat, reduce the onset risk of angiocardiopathy, become technical staff's urgent problem.
Summary of the invention
The object of the present invention is to provide a kind of extraction process of new enzymolysis bone calcium, described technique can better be removed fat in the sclerotin than prior art.
Another object of the present invention provides a kind of more healthy calcium supplementing product production technology, and the calcium supplementing product that described technique makes has lower fat content.
Another object of the present invention provides a kind of calcium supplementing product with market competitiveness, and described product does not increase production cost because of the design of degreasing process,
For achieving the above object, the present invention adopts following technical proposal to realize.
The present invention adopts three degreasing modes to remove animal oil, is 60～80 ℃ for the first time, 45～75min; 80～90 ℃ for the second time, 30min; The lipase enzymolysis for the third time.Three degreasings also have the effect of sterilization simultaneously except having the fatty effect of removing in the sclerotin.For strengthening degreasing effect, the present invention preferably is broken to bone meal 5～40mm particle, the too small production cost that then increases of particle, and the excessive degrease effect of particle is relatively poor.The inventor determines finally that through test of many times and experience 5～40mm granulometric range reaches the best cost performance of bone fat and cost.
After the alcohol steep degreasing, the present invention also adopts the hot-water soak rinse method to remove the fat that exists in the aggregate; Again with 4000～8000r/min, centrifugal 30min can remove most fat in the aggregate like this after the rinsing.
For further removing remaining fat, process choice of the present invention again adds alkaline lipase and carries out fat remaining in the enzymolysis aggregate after centrifugal.Alkaline lipase A, B account for 0.1～0.4%, 20～30 ℃ of aggregate weight ratios by 1.5～3: 1 compound lipases that mixes, hydrolysis 40～60min.Behind enzymolysis, secondary centrifuging is further removed remaining trace fat.
Through behind the above-mentioned defatting step, the calcium that adopts two step enzymatic isolation methods to extract in the aggregate is plain.The first step is when aggregate is cooled to 45～55 ℃, adds trypsase, hydrolysis 1～2h.Second one is to add neutral proteinase in aggregate, hydrolysis 1～2h.After the secondary enzymolysis, separating liquid, high-temperature inactivation, drying can obtain described enzymolysis bone calcium.
Preferably, in the present invention's enzymolysis bone first time calcium, the trypsin hydrolysis temperature is preferably 50 ℃.
It is 0.04～0.08% for good that hydrolysis aggregate used trypsase accounts for the aggregate weight ratio.
The alkaline lipase that alkaline lipase A is produced by the Penicillium expansion PF868 of bioengineering institute of Fujian Normal University development, Co., Ltd provides by the green little health bioengineering in Shenzhen; Alkaline lipase B adopts biotechnology to be made with extra care and the alkaline lipase of generation by fungi fermentation, is provided by Haining Jin Chao Industrial Co., Ltd..
The various formulations that auxiliary material such as starch, dextrin, lactose, microcrystalline cellulose, HPMC, polyethylene glycol, dolomol, superfine silica gel powder, xylitol, lactitol, glucose, glycine, sweet mellow wine, glycine etc. are mixed on composition of the present invention and any or more than one pharmacies, for example, can be made into tablet, sustained release tablets, dripping pill, granule, pulvis, capsule, fine granule.Preferred dosage form is tablet or granule.
Adopt in the bone meal that degreasing process of the present invention makes, fat is completely removed substantially, so that fat content is lower than 0.1% in the calcium agent that enzymolysis obtains.Product safety and health more concerning the consumer that angiocardiopathy is arranged, but financial burden therefore increased again.
The specific embodiment
Embodiment 1
Get animal skeleton and be crushed to the 40mm particle, add ethanol, 80 ℃, lixiviate 75min abandons supernatant; 90 ℃ of hot-water soak rinsing 30min, with aggregate 8000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 5 are transferred pH value 10, add alkaline lipase A, B by the compound lipases of mixing in 1.5: 1, account for 0.4%, 30 ℃ of aggregate weight ratio, and hydrolysis 60min is centrifugal, abandons supernatant; Add water, aggregate and water w/v 1: 5,100 ℃, 10min is cooled to 55 ℃, adds trypsase, and accounting for the aggregate weight ratio is 0.08%, stirs 2h; Add neutral proteinase, accounting for the aggregate weight ratio is 0.1%, stirs 2h, separating liquid, and high-temperature inactivation, drying, and get final product.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.09%.
Embodiment 2
Get animal skeleton and be crushed to the 5mm particle, add ethanol, 60 ℃, lixiviate 45min abandons supernatant; 80 ℃ of hot-water soak rinsing 30min, with aggregate 4000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 3 are transferred pH value 8, add alkaline lipase A, B by the compound lipases of mixing in 3: 1, account for 0.1%, 20 ℃ of aggregate weight ratio, and hydrolysis 40min is centrifugal, abandons supernatant; Add water, aggregate and water w/v 1: 3,90 ℃, 10min is cooled to 45 ℃, adds trypsase, and accounting for the aggregate weight ratio is 0.04%, stirs 1h; Add neutral proteinase, accounting for the aggregate weight ratio is 0.05%, stirs 1h, separating liquid, and high-temperature inactivation, drying, and get final product.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.08%.
Embodiment 3
Get animal skeleton and be crushed to the 20mm particle, add ethanol, 70 ℃, lixiviate 60min abandons supernatant; 85 ℃ of hot-water soak rinsing 30min, with aggregate 8000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 4 are transferred pH value 9, add alkaline lipase A, B by the compound lipases of mixing in 2: 1, account for 0.3%, 25 ℃ of aggregate weight ratio, and hydrolysis 50min is centrifugal, abandons supernatant; Add water, aggregate and water w/v 1: 4,95 ℃, 10min is cooled to 50 ℃, adds trypsase, and accounting for the aggregate weight ratio is 0.06%, stirs 1.5h; Add neutral proteinase, accounting for the aggregate weight ratio is 0.08%, stirs 1.5h, separating liquid, and high-temperature inactivation, drying, and get final product.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.08%.
Embodiment 4
Get animal skeleton and be crushed to the 10mm particle, add ethanol, 80 ℃, lixiviate 75min abandons supernatant; 90 ℃ of hot-water soak rinsing 30min, with aggregate 6000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 3.5 are transferred pH value 8.5, add alkaline lipase A, B by the compound lipases of mixing in 2: 1, account for 0.2%, 30 ℃ of aggregate weight ratio, and hydrolysis 60min is centrifugal, abandons supernatant; Add water, aggregate and water w/v 1: 3.5,95 ℃, 10min is cooled to 55 ℃, adds trypsase, and accounting for the aggregate weight ratio is 0.05%, stirs 2h; Add neutral proteinase, accounting for the aggregate weight ratio is 0.09%, stirs 2h, separating liquid, and high-temperature inactivation, drying, and get final product.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.07%.
Embodiment 5
Get animal skeleton and be crushed to the 25mm particle, add ethanol, 75 ℃, lixiviate 65min abandons supernatant; 86 ℃ of hot-water soak rinsing 30min, with aggregate 8000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 4 are transferred pH value 9.5, add alkaline lipase A, B by the compound lipases of mixing in 2.8: 1, account for 0.3%, 30 ℃ of aggregate weight ratio, and hydrolysis 50min is centrifugal, abandons supernatant; Add water, aggregate and water w/v 1: 4.5,100 ℃, 10min is cooled to 55 ℃, adds trypsase, and accounting for the aggregate weight ratio is 0.07%, stirs 2h; Add neutral proteinase, accounting for the aggregate weight ratio is 0.06%, stirs 1h, separating liquid, and high-temperature inactivation, drying, and get final product.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.09%.
Embodiment 6
Get animal skeleton and be crushed to the 35mm particle, add ethanol, 78 ℃, lixiviate 70min abandons supernatant; 87 ℃ of hot-water soak rinsing 30min, with aggregate 7000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 5 are transferred pH value 8, add alkaline lipase A, B by the compound lipases of mixing in 1.8: 1, account for 0.25%, 20 ℃ of aggregate weight ratio, and hydrolysis 50min is centrifugal, abandons supernatant; Add water, aggregate and water w/v 1: 3,100 ℃, 10min is cooled to 45 ℃, adds trypsase, and accounting for the aggregate weight ratio is 0.07%, stirs 2h; Add neutral proteinase, accounting for the aggregate weight ratio is 0.1%, stirs 2h, separating liquid, and high-temperature inactivation, drying, and get final product.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.08%.
Embodiment 7
Get animal skeleton and be crushed to the 10mm particle, add ethanol, 60 ℃, lixiviate 75min abandons supernatant; 88 ℃ of hot-water soak rinsing 30min, with aggregate 5000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 4 are transferred pH value 10, add alkaline lipase A, B by the compound lipases of mixing in 3: 1, account for 0.35%, 24 ℃ of aggregate weight ratio, and hydrolysis 45min is centrifugal, abandons supernatant; Add water, aggregate and water w/v 1: 4,95 ℃, 10min is cooled to 50 ℃, adds trypsase, and accounting for the aggregate weight ratio is 0.08%, stirs 1h; Add neutral proteinase, accounting for the aggregate weight ratio is 0.05%, stirs 1h, separating liquid, and high-temperature inactivation, drying, and get final product.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.07%.
Embodiment 8
Get animal skeleton and be crushed to the 15mm particle, add ethanol, 80 ℃, lixiviate 75min abandons supernatant; 89 ℃ of hot-water soak rinsing 30min, with aggregate 8000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 5 are transferred pH value 8.5, add alkaline lipase A, B by the compound lipases of mixing in 2.6: 1, account for 0.14%, 30 ℃ of aggregate weight ratio, and hydrolysis 60min is centrifugal, abandons supernatant; Add water, aggregate and water w/v 1: 4.5,100 ℃, 10min is cooled to 55 ℃, adds trypsase, and accounting for the aggregate weight ratio is 0.06%, stirs 2h; Add neutral proteinase, accounting for the aggregate weight ratio is 0.1%, stirs 2h, separating liquid, and high-temperature inactivation, drying, and get final product.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.08%.
Embodiment 9
Get animal skeleton and be crushed to the 30mm particle, add ethanol, 75 ℃, lixiviate 55min abandons supernatant; 85 ℃ of hot-water soak rinsing 30min, with aggregate 8000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 5 are transferred pH value 8, add alkaline lipase A, B by the compound lipases of mixing in 3: 1, account for 0.35%, 28 ℃ of aggregate weight ratio, and hydrolysis 60min is centrifugal, abandons supernatant; Add water, aggregate and water w/v 1: 4,94 ℃, 10min is cooled to 55 ℃, adds trypsase, and accounting for the aggregate weight ratio is 0.05%, stirs 1h; Add neutral proteinase, accounting for the aggregate weight ratio is 0.1%, stirs 2h, separating liquid, and high-temperature inactivation, drying, and get final product.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.09%.
Embodiment 10
Get animal skeleton and be crushed to the 20mm particle, add ethanol, 80 ℃, lixiviate 60min abandons supernatant; 84 ℃ of hot-water soak rinsing 30min, with aggregate 6000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 5 are transferred pH value 10, add alkaline lipase A, B by the compound lipases of mixing in 3: 1, account for 0.3%, 30 ℃ of aggregate weight ratio, and hydrolysis 60min is centrifugal, abandons supernatant; Add water, aggregate and water w/v 1: 5,90 ℃, 10min is cooled to 50 ℃, adds trypsase, and accounting for the aggregate weight ratio is 0.07%, stirs 2h; Add neutral proteinase, accounting for the aggregate weight ratio is 0.05%, stirs 1, separating liquid, and high-temperature inactivation, drying, and get final product.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.09%.
Embodiment 11
Bone is crushed to the 40mm particle, adds ethanol, 80 ℃, lixiviate 75min abandons supernatant; 83 ℃ of hot-water soak rinsing 30min, with aggregate 8000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 5 are transferred pH value 10, add alkaline lipase A, B by the compound lipases of mixing in 1.7: 1, account for 0.1%, 30 ℃ of aggregate weight ratio, and hydrolysis 60min is centrifugal, abandons supernatant; Add water, aggregate and water w/v 1: 3,100 ℃, 10min is cooled to 55 ℃, adds trypsase, and accounting for the aggregate weight ratio is 0.04%, stirs 2h; Add neutral proteinase, accounting for the aggregate weight ratio is 0.1%, stirs 2h, separating liquid, and high-temperature inactivation, drying, and get final product.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.07%.
Embodiment 12
Bone is crushed to the 5mm particle, adds ethanol, 60 ℃, lixiviate 45min abandons supernatant; 82 ℃ of hot-water soak rinsing 30min, with aggregate 4000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 3 are transferred pH value 8, add alkaline lipase A, B by the compound lipases of mixing in 1.9: 1, account for 0.1%, 20 ℃ of aggregate weight ratio, and hydrolysis 60min is centrifugal, abandons supernatant; Add water, aggregate and water w/v 1: 3,90 ℃, 10min is cooled to 55 ℃, adds trypsase, and accounting for the aggregate weight ratio is 0.08%, stirs 2h; Add neutral proteinase, accounting for the aggregate weight ratio is 0.1%, stirs 2h, separating liquid, and high-temperature inactivation, drying, and get final product.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.07%.
Embodiment 13
Bone is crushed to the 30mm particle, adds ethanol, 75 ℃, lixiviate 60min abandons supernatant; 81 ℃ of hot-water soak rinsing 30min, with aggregate 8000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 4 are transferred pH value 9, add alkaline lipase A, B by the compound lipases of mixing in 2.1: 1, account for aggregate than 0.4%, 30 ℃, and hydrolysis 60min is centrifugal, abandons supernatant; Add water, aggregate and water w/v 1: 3.5,95 ℃, 10min is cooled to 50 ℃, adds trypsase, and accounting for the aggregate weight ratio is 0.08%, stirs 2h; Add neutral proteinase, accounting for the aggregate weight ratio is 0.1%, stirs 2h, separating liquid, and high-temperature inactivation, drying, and get final product.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.09%.
Embodiment 14
Bone is crushed to the 10mm particle, adds ethanol, 80 ℃, lixiviate 75min abandons supernatant; 84 ℃ of hot-water soak rinsing 30min, with aggregate 8000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 5 are transferred pH value 10, add alkaline lipase A, B by the compound lipases of mixing in 2.2: 1, account for 0.4%, 30 ℃ of aggregate weight ratio, and hydrolysis 60min is centrifugal, abandons supernatant; Add water, aggregate and water w/v 1: 5,100 ℃, 10min is cooled to 50 ℃, adds trypsase, and accounting for the aggregate weight ratio is 0.08%, stirs 2h; Add neutral proteinase, accounting for the aggregate weight ratio is 0.08%, stirs 1h, separating liquid, and high-temperature inactivation, drying, and get final product.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.08%.
Embodiment 15
Bone is crushed to the 25mm particle, adds ethanol, 80 ℃, lixiviate 65min abandons supernatant; 84 ℃ of hot-water soak rinsing 30min, with aggregate 6000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 4 are transferred pH value 8.5, add alkaline lipase A, B by the compound lipases of mixing in 2.3: 1, account for 0.3%, 30 ℃ of aggregate weight ratio, and hydrolysis 60min is centrifugal, abandons supernatant; Add water, aggregate and water w/v 1: 5,95 ℃, 10min is cooled to 50 ℃, adds trypsase, and accounting for the aggregate weight ratio is 0.07%, stirs 1h; Add neutral proteinase, accounting for the aggregate weight ratio is 0.08%, stirs 1h, separating liquid, and high-temperature inactivation, drying, and get final product.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.08%.
Claims (10)
1. a bone calcium extracting method that reduces fat content comprises the steps:
(1) bone is pulverized, the lixiviate oil of boning is abandoned supernatant;
(2) aggregate after the lixiviate is soaked rinsing, centrifugal, abandon supernatant;
(3) aggregate after centrifugal is added water, transfer pH value, add the alkaline fat enzyme hydrolysis, centrifugal, abandon supernatant;
(4) aggregate after centrifugal in the step (3) is added the water boiling, add trypsin hydrolysis after the cooling;
(5) hydrolyzate in the step (4) adds the hydrolysis of neutral proteinase secondary again, and high-temperature inactivation is drying to obtain.
2. bone calcium extracting method according to claim 1 is characterized in that, described step (1) particles of aggregates 5～40mm adds ethanol, 60～80 ℃, 45～75min.
3. bone calcium extracting method according to claim 1 is characterized in that, in the described step (2) with the rinsing of particles of aggregates hot water, 4000～8000r/min then, centrifugal 30min.
4. bone calcium extracting method according to claim 1 is characterized in that, aggregate and water w/v 1: 3～5 in the described step (3) are transferred pH value 8～10, add the compound lipases that alkaline lipase A, B mix.
5. bone calcium extracting method according to claim 4 is characterized in that, described compound lipases neutral and alkali lipase A, B weight ratio are 1.5～3: 1.
6. according to claim 4 or 5 described bone calcium extracting methods, it is characterized in that described compound lipases accounts for aggregate weight ratio 0.1～0.4%.
7. bone calcium extracting method according to claim 1 is characterized in that, aggregate and water w/v 1: 3～5 in the described step (4), 90～100 ℃, 10min is cooled to 45～55 ℃, add trypsase, accounting for the aggregate weight ratio is 0.04～0.08%, stirs 1～2h.
8. bone calcium extracting method according to claim 7 is characterized in that, described hydrolysis temperature is preferably 50 ℃.
9. animal bone calcium extracting method according to claim 1 is characterized in that, adds neutral proteinase in the described step (5), and accounting for the aggregate weight ratio is 0.05-0.1%, stirs 1～2h.
10. the BGP by bone extraction preparation is characterized in that, adopts the following methods preparation: bone is crushed to 5～40mm particle, adds ethanol, 60～80 ℃, lixiviate 45～75min abandons supernatant; Aggregate hot-water soak rinsing, water temperature 80-90 ℃, soaked 30 minutes, with aggregate 4000～8000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 3～5 are transferred pH value 8～10, add alkaline lipase A, B by 1.5～3: 1 compound lipases that mixes, account for 0.1～0.4%, 20～30 ℃ of aggregate weight ratios, hydrolysis 40～60min, centrifugal, abandon supernatant; Add water, 1: 3～5,90～100 ℃ of aggregate and water w/vs, 10min is cooled to 45～55 ℃, adds trypsase, and accounting for the aggregate weight ratio is 0.04～0.08%, stirs 1～2h; Add neutral proteinase, accounting for the aggregate weight ratio is 0.05-0.1%, stirs 1～2h, separating liquid, and high-temperature inactivation, drying, and get final product.
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