CN102861355A - Functional wound dressing capable of accelerating wound healing and preparation method thereof - Google Patents
Functional wound dressing capable of accelerating wound healing and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a functional wound dressing capable of accelerating wound healing and a preparation method thereof. The functional wound dressing is a polyurethane (PU)/polyvinylidene fluoride (PVDF) electric spinning membrane which is made of PU and PVDF in a mass ratio of 1:3-3:1, the functional wound dressing is prepared by using a method of electrostatic spinning, and the thickness of the PU/PVDF electric spinning membrane is 150-250 mum. The functional wound dressing can use electrical stimulatory effects produced by piezoelectric polymer PVDF to accelerate wound healing, has the advantages of being good in biocompatibility, simple and convenient to prepare, low in cost and the like, and is wide in market development prospects.
Description
Technical field
The present invention relates to a kind of dressing procucts for the wound repair field and preparation method thereof, belong to medical macromolecular materials.
Background technology
Wound dressing is to play the wounds such as temporary protection burn, ulcer, wound, prevents from polluting, and promotes the medical material of healing, and using wound dressing is to carry out one of effective means of trauma care.Along with the quickening of modern life rhythm and the continuous growth of requirements of health care, the demand of wound dressing is also increasing year by year.The market of present domestic wound dressing product is huge, and according to the research of the logical people's commercial information company limited (ACMR) of Beijing China, the market demand of Chinese wound dressing in 2010 reaches about 40,000,000,000 yuan.The dressing that has gone on the market now or researched and developed is broadly divided into several large classes such as traditional dressing, natural dressing, synthetic dressing, organizational project dressing and medicines dressing according to the material difference, wherein power of influence is maximum, Applicative time is the longest, occupy at present the traditional gauze class dressing of remaining of most of market.Along with to the deepening continuously of pathophysiological mechanism research in the wound healing process, people recognize the purpose of using dressing be not only for flap coverage to prevent and to infect that desirable dressing also should be able to promote wound healing hemorrhage.In order more effectively to treat wound in the protection wound surface, many dressing have added ingredient in use, thereby become functional dressing.Although in all kinds of casting product, the market share minimum that functional dressing is at present shared, the market share in 2011 only is about 6.50%, this series products has represented the developing direction of wound dressing, presents the trend that increases rapidly.Because the domestic dressing enterprise industrial structure falls behind, the reasons such as the advanced technology of independent intellectual property right is less, research and development insufficient investment, the domestic market of functional dressing is basically take import as main at present, and the technology of this series products of developed country maintains the leading position, and product price is also higher.In addition, preparation technology's relative complex of medicine carrying functional dressing, cost are higher, have limited to a certain extent its extensive use.Therefore research and develop a kind of have independent intellectual property right and prepare functional wound dressing easy, with low cost have wide market prospect.
Electricity irritation can promote the expression and secretion of multiple somatomedin, affects the functions such as propagation, differentiation and regeneration of cell.Electricity irritation is used promoting that the fields such as fracture repair and neuranagenesis, treatment osteoporosis, chronic ulcer are existing, but the research and development that are applied to wound dressing there is not yet report.Piezoelectric substance can be for the preparation of the biomaterial with electricity irritation effect.When referring to be subject to External Force Acting, piezoelectric substance can between these material both ends of the surface, produce the crystalline material of electric potential difference (piezoelectric effect).Piezoelectric substance is divided into inorganic and organic two large classes, and the former is divided into again piezoquartz (monocrystal) and piezoelectric ceramics (polycrystal), respectively take quartz crystal and barium titanate ceramics as representative; The latter claims again piezopolymer, take polyvinylidene fluoride (PVDF) as representative.Piezoelectric substance is in the application of technical field of biological material, and modal is that piezoelectric ceramics is applied to bone tissue engineer.Owing to a little less than strong, the elasticity of piezoelectric ceramics rigidity, is applied to the soft tissues such as blood vessel, nerve, fascia and there is not yet report, so piezopolymer PVDF has received a lot of concerns in recent years.PVDF is a kind of heteromorphic polymer, and common crystal formation mainly contains three kinds of α, β and γ.Usually the synthetic PVDF of Raolical polymerizable mainly is made of alpha-crystal form, does not show piezoelectricity.Alpha-crystal form can change the very strong beta crystal of piezoelectricity into by mechanical stretching or electric field polarization.Electrostatic spinning is a kind ofly to stretch by polymer solution or the melt of forceful electric power field force to the static electrification lotus, thereby obtains the processing technique of micron or nano level superfine fiber.The research report is arranged, can directly obtain the PVDF fiber of beta crystal by electrostatic spinning from PVDF solution.
Summary of the invention
The present invention is take the polyurethane (PU) of different quality ratio and piezopolymer polyvinylidene fluoride (PVDF) as raw material, and the method by electrostatic spinning provides a kind of functional wound dressing that accelerates wound healing.Wherein, PU plays in functional wound dressing increases elastic effect.And then learn experiment and animal is tested at body by cell in vitro, verified the effect of biocompatibility, electricity irritation effect and the acceleration wound healing of this functional wound dressing.
The present invention also provides the preparation method of the described functional wound dressing of preparation.
For achieving the above object, the present invention takes following measure:
The functional wound dressing that the present invention can accelerate wound healing is a kind of PU/PVDF electrospinning film, and wherein the mass ratio of PU and PVDF is 1:3 ~ 3:1; The thickness of described PU/PVDF electrospinning film is 150 ~ 250 μ m.
The above-mentioned preparation method of accelerating the functional wound dressing of wound healing adopts electrostatic spinning technique, specifically, may further comprise the steps:
(1) PU and PVDF are dissolved in respectively in the mixed solvent of oxolane (THF) and dimethyl formamide (DMF) and are mixed with PU spinning liquid and PVDF spinning liquid; The volume ratio of THF and DMF is 1:1 in the described mixed solvent; The mass volume ratio of solute and solvent is 12% in described PU spinning liquid and the PVDF spinning liquid;
(2) PU spinning liquid and the PVDF spinning liquid with preparation in the step (1) is that 1:3 ~ 3:1 mixes by volume, is mixed with the PU/PVDF blend spinning liquid of different proportion composition;
(3) the PU/PVDF blend spinning liquid with the different proportion composition of preparation in the step (2) obtains PU/PVDF electrospinning film by electrostatic spinning respectively;
(4) the PU/PVDF electrospinning film with preparation in the step (3) soaked 10 minutes in 70% alcoholic solution first, soaked 30 minutes with normal saline again, with this carry out disinfection and thoroughly remove in the PU/PVDF electrospinning film may be residual solvent composition after, namely get the functional wound dressing that can accelerate wound healing.
In above-mentioned preparation method, the involved operating parameter of electrostatic spinning process is as follows in the described step (3): the syringe pump flow velocity is 0.8 ~ 1.0 ml/h, the high voltage direct current generator output voltage is 12 ~ 18 kV, and the distance between syringe needle and the dash receiver is 15 ~ 20 cm.
In above-mentioned preparation method, used solvent and polymer raw material are pharmaceutical grade, and THF and DMF all can be dissolved in the second alcohol and water with any ratio.
Advantage of the present invention is to have prepared a kind of functional wound dressing that accelerates wound healing by simple technique, compares with existing dressing procucts to have following characteristics:
(1) preparation technology is easy, with low cost;
(2) PU and PVDF all are bio-inert material, and be nontoxic to animal body, and the micro/nano level aperture of PU/PVDF electrospinning film can prevent when guaranteeing breathability that cambium from growing into, thereby avoids the wound surface adhesion to occur;
(3) piezoelectricity of PU/PVDF electrospinning film makes it can produce the electricity irritation effect when deformation occurs, thereby accelerates wound healing.
Description of drawings
Fig. 1 is the PU/PVDF electrospinning film of different proportion composition;
Fig. 2 is the X ray diffracting spectrum of PVDF powder, PU/PVDF electrospinning film and PU electrospinning film;
Fig. 3 is the thermal analysis curue spectrum of PVDF powder, PU/PVDF electrospinning film and PU electrospinning film;
Fig. 4 is the infrared light collection of illustrative plates of PVDF powder, PU/PVDF electrospinning film and PU electrospinning film;
Fig. 5 cultivated 1 day, 3 days, 5 days behind the PU/PVDF electrospinning film inoculation NIH3T3 cell, used respectively the result (length of the scale 100 μ m) of scanning electron microscope and confocal laser scanning microscope;
Fig. 6 is the propagation situation (* p＜0.05) that NIH3T3 cell (constituent material is tissue culturing polystyrene) at the bottom of PU/PVDF electrospinning film and 24 porocytes cultivation plate hole was cultivated respectively 1 day, 3 days, 5 days, 7 days;
Fig. 7 is that the PU/PVDF electrospinning film of non-latter, the PU electrospinning film of latter and 0,6,12,24 hours the cell of PU/PVDF electrospinning theca cell layer scored area of latter converge situation (length of the scale 200 μ m);
Fig. 8 is that the average cell for the PU/PVDF electrospinning theca cell layer scored area of the PU electrospinning film of the PU/PVDF electrospinning film of non-latter, latter and latter converges speed (* p＜0.05) in 24 hours;
Fig. 9 is the situation (length of the scale 100 μ m) that the PU/PVDF electrospinning theca cell layer of the PU electrospinning film of PU/PVDF electrospinning film, latter of non-latter and latter adheres to Hoechst 33342 staining cells;
Figure 10 is the quantity (* p＜0.05) that the PU/PVDF electrospinning theca cell layer of the PU electrospinning film of PU/PVDF electrospinning film, latter of non-latter and latter adheres to Hoechst 33342 staining cells;
Figure 11 is the mrna expression level (* p＜0.05) of PU/PVDF electrospinning theca cell layer collagen protein (Colla1), elastin laminin (Eln) and the fibronectin (Fn) of the PU electrospinning film of PU/PVDF electrospinning film, latter of non-latter and latter;
Figure 12 is the PU/PVDF electrospinning film of non-latter, PU electrospinning film and PU/PVDF electrospinning theca cell layer Colla1, the Eln of latter and the protein expression level (* p＜0.05) of Fn of latter.
The specific embodiment
With reference to Figure of description, and further specify with the following embodiments the present invention, should be understood that Figure of description and following embodiment only are used for explanation the present invention, and unrestricted the present invention.
The preparation of embodiment 1 PU electrospinning film
(1) THF and DMF are pressed the equal-volume mixing, be mixed with the THF/DMF mixed solvent;
(2) PU is dissolved in the THF/DMF mixed solvent of step (1) preparation, under room temperature, is stirred to fully dissolving, obtain the PU spinning liquid; Wherein, the mass volume ratio of solute and solvent is 12% in the PU spinning liquid;
(3) the PU spinning liquid with step (2) preparation carries out electrostatic spinning, and it is 0.8 ~ 1.0 ml/h that the syringe pump flow velocity is set, and the high voltage direct current generator output voltage is 12 ~ 18kV, and the distance of adjusting between syringe needle and the dash receiver is 15 ~ 20 cm;
(4) by control time and mobile reception plate, prepare PU electrospinning film, its thickness is 150 ~ 250 μ m.
The preparation of embodiment 2 PVDF electrospinning films
(1) THF and DMF are pressed the equal-volume mixing, be mixed with the THF/DMF mixed solvent;
(2) PVDF is dissolved in the THF/DMF mixed solvent of step (1) preparation, under room temperature, is stirred to fully dissolving, obtain the PVDF spinning liquid; Wherein, the mass volume ratio of solute and solvent is 12% in the PVDF spinning liquid;
(3) the PVDF spinning liquid with step (2) preparation carries out electrostatic spinning, and it is 0.8 ~ 1.0 ml/h that the syringe pump flow velocity is set, and the high voltage direct current generator output voltage is 12 ~ 18kV, and the distance of adjusting between syringe needle and the dash receiver is 15 ~ 20 cm;
(4) by control time and mobile reception plate, prepare PVDF electrospinning film, its thickness is 150 ~ 250 μ m.
The preparation of embodiment 3 PU/PVDF electrospinning films
(1) THF and DMF are pressed the equal-volume mixing, be mixed with the THF/DMF mixed solvent;
(2) respectively PU and PVDF are dissolved in the THF/DMF mixed solvent of step (1) preparation, under room temperature, are stirred to fully dissolving, obtain PU spinning liquid and PVDF spinning liquid; Wherein, the mass volume ratio of solute and solvent is 12% in PU spinning liquid and the PVDF spinning liquid;
The volume ratio of (3) PU spinning liquid and the PVDF spinning liquid of step (2) preparation being pressed respectively 1:3 ~ 3:1 is mixed, and stirs under room temperature, is mixed with the PU/PVDF blend spinning liquid of different proportion composition;
The PU/PVDF blend spinning liquid of the different proportion composition of (4) step (3) being prepared carries out electrostatic spinning, it is 0.8 ~ 1.0 ml/h that the syringe pump flow velocity is set, the high voltage direct current generator output voltage is 12 ~ 18kV, and the distance of adjusting between syringe needle and the dash receiver is 15 ~ 20 cm;
(5) by control time and mobile reception plate, prepare the PU/PVDF electrospinning film of different proportion composition, its thickness is that 150 ~ 250 μ m(are referring to accompanying drawing 1).
The sign of embodiment 4 PU/PVDF electrospinning films and matched group
(1) measure respectively tensile strength and the elongation at break of PU/PVDF electrospinning film of the different proportion composition of the PVDF electrospinning film of PU electrospinning film, embodiment 2 preparations of embodiment 1 preparation and embodiment 3 preparations, measurement result sees Table 1.One factor analysis of variance shows respectively to organize all has significant difference (p＜0.05) between data, and the mechanical property that shows PU/PVDF electrospinning film improves with the increase of PU proportion.
Table 1 tensile strength and elongation at break characterization result
(2) measure respectively the piezoelectric modulus (d of PU/PVDF electrospinning film of the different proportion composition of the PVDF electrospinning film of PU electrospinning film, embodiment 2 preparations of embodiment 1 preparation and embodiment 3 preparations
33), measurement result sees Table 2.One factor analysis of variance shows respectively to organize all has significant difference (p＜0.05) between data, and the piezoelectric property that shows PU/PVDF electrospinning film improves with the increase of PVDF proportion.
Table 2 piezoelectric modulus (d
33) characterization result
(3) measure respectively aperture and the fibre diameter of PU/PVDF electrospinning film of the different proportion composition of the PVDF electrospinning film of PU electrospinning film, embodiment 2 preparations of embodiment 1 preparation and embodiment 3 preparations, measurement result sees Table 3.One factor analysis of variance shows respectively organizes there was no significant difference between data (p〉0.05), show that the aperture of PU/PVDF electrospinning film is less than the diameter of general cell, therefore PU/PVDF electrospinning film can prevent when guaranteeing breathability that cambium from growing into, thereby avoids the wound surface adhesion to occur.
Table 3 aperture and fibre diameter characterization result
(4) adopt the analytical methods such as X-ray diffraction, means of differential scanning calorimetry and infrared spectrum that the PU/PVDF electrospinning film of embodiment 3 preparations is characterized, matched group is the PU electrospinning film of PVDF powder and embodiment 1 preparation.
Fig. 2 is the X ray diffracting spectrum of PU/PVDF electrospinning film and matched group.In the X ray diffracting spectrum of PVDF powder, the diffraction maximum that is positioned at 17.7 °, 18.4 °, 19.9 ° and 26.4 ° corresponds respectively to α 100, α 020, α 110 and α 021 crystal face of alpha-crystal form, all the other have no obvious diffraction maximum, illustrate that the PVDF powder without electrostatic spinning processing mainly is made of alpha-crystal form.And in the diffracting spectrum of PU/PVDF electrospinning film, the intensity of above-mentioned alpha-crystal form diffraction maximum greatly weakens even vanishes from sight, and the substitute is the diffraction maximum that is positioned at 20.6 ° and 36.5 °, corresponds respectively to β 110/ β 200 and β 020 crystal face of beta crystal.Owing to contain simultaneously the PU composition in the electrospinning film, so matched group also comprises single PU electrospinning film, and purpose is that the diffraction maximum that may exist is overlapping to characterize the impact that causes on the PVDF crystal formation in order to get rid of.In the diffracting spectrum of PU electrospinning film, have to be positioned at 19.7 ° single diffraction maximum.Therefore, PU/PVDF electrospinning film is positioned at overlapping (because the latter's intensity is stronger thereby covered the former) that 20.6 ° diffracted primary peak should be PU diffraction maximum and beta crystal PVDF (β 110/ β 200 crystal faces) diffraction maximum; The disappearance of the formation of 36.5 ° of diffraction maximums and 17.7 °, 18.4 °, 19.9 ° and 26.4 ° diffraction maximums is then irrelevant with the diffraction of PU composition, has reflected the transformation of alpha-crystal form PVDF to beta crystal PVDF.
Fig. 3 is the thermal analysis curue spectrum of PU/PVDF electrospinning film and matched group.The thermal analysis curue spectrum of PVDF powder and PU/PVDF electrospinning film all shows single endothermic peak, but the temperature that latter's endothermic peak occurs is high 4 ℃ than the former.The same with X-ray diffraction analysis, endothermic peak in the PU/PVDF electrospinning film thermal analysis curue spectrum also may be the overlapping of two kinds of composition endothermic peaks, therefore single PU support has also been carried out differential scanning calorimetric analysis, the result show its in the temperature range of test without obvious endothermic peak, the endotherm peak temperature that PU/PVDF electrospinning film be described is due to crystal formation owing to PVDF changes than PVDF powder height.Beta crystal PVDF is the alltrans molecular configuration, and strand is planar zigzag structure, and crystal accumulation is tight than other crystal formations; The structure cell density of beta crystal PVDF is also high than other crystal formations in addition, and these factors make beta crystal PVDF have higher melt temperature, so the endotherm peak temperature of PU/PVDF electrospinning film is because the crystal formation of PVDF is changed into due to the β type by the α type than PVDF powder height.
Fig. 4 is the infrared light collection of illustrative plates of PU/PVDF electrospinning film and matched group.In the infrared spectrum of PVDF powder, be positioned at 535,618 and 764 cm
-1Absworption peak corresponding to the CF of alpha-crystal form PVDF
2Bending vibration is positioned at 796 cm
-1Absworption peak corresponding to the CF of alpha-crystal form PVDF
2Rocking vibration.In the infrared spectrum of PU/PVDF electrospinning film, be positioned at 510 cm
-1Absworption peak corresponding to the CF of beta crystal PVDF
2Bending vibration is positioned at 841 cm
-1Absworption peak corresponding to the CH of beta crystal PVDF
2Rocking vibration, the characteristic peak of above-mentioned alpha-crystal form PVDF then greatly weakens.The same with the thermal analysis curue spectrum with X ray diffracting spectrum, owing to contain simultaneously the PU composition in the electrospinning film, for getting rid of the overlapping impact that PVDF crystal formation sign is caused of the absworption peak that may exist, single PU electrospinning film has been carried out infrared spectrum analysis, the result show its in the beam location at PVDF characteristic peak place all without obvious absworption peak, illustrate that the disappearance of alpha-crystal form PVDF characteristic peak in the PU/PVDF electrospinning film and the enhancing of beta crystal PVDF characteristic peak and the absorption of PU composition have nothing to do.
Comprehensively the analysis result of Fig. 2-4 shown that through electrostatic spinning processing, the alpha-crystal form PVDF in the PVDF powder changes the beta crystal PVDF in the PU/PVDF electrospinning film basically into, this is the crystal structure basis that PU/PVDF electrospinning film has piezoelectricity.
(1) the PU/PVDF electrospinning film that embodiment 3 is prepared soaks 10 minutes in 70% alcoholic solution after, use again phosphate buffer (PBS) to soak 30 minutes, to reach the purpose of solvent composition that may be residual in sterilization and the thorough removal PU/PVDF electrospinning film;
(2) former generation human fibroblasts (HSF) is seeded to respectively on each PU/PVDF electrospinning film, observes and proliferation experiment confirms that HSF all can normal growth and propagation (referring to accompanying drawing 5-6) on each PU/PVDF electrospinning film by cellular morphology;
(3) each PU/PVDF electrospinning film is implanted respectively Sprague-Dawley(SD) subcutaneous rat, the behavior performance of rat no abnormality seen and obvious toxic reaction; With implant before compare, the PU/PVDF electrospinning film of taking-up is in appearance without significant change, without the change such as damaged, flexible; The cell on PU/PVDF electrospinning film surface is the fibroblast of normal growth.
The electricity irritation effect of embodiment 6 PU/PVDF electrospinning films is to the stimulation of In vitro culture HSF
(1) the PU/PVDF electrospinning film with embodiment 3 preparations sticks on the deformable silica gel elastica of BioFlex culture plate bottom with using respectively 5% polylactic acid (PLLA)/dioxane (Dioxane) glue close and firm;
(2) PU/PVDF electrospinning film is soaked 10 minutes in 70% alcoholic solution after, soaked 30 minutes with PBS again, to reach sterilization and thoroughly to remove the purpose of solvent composition that may be residual in the PU/PVDF electrospinning film;
(3) HSF is seeded to respectively on each PU/PVDF electrospinning film, when treating that attached cell degree of converging reaches 100%, amplitude and frequency that pellosil is set by FX-4000T cultured cell in vitro afterburner system are respectively 8%, 0.5 Hz, continue to cultivate after 1 day, by methods such as scratch experiment, adhesion experiment, quantitative PCR and Western blot, the electricity irritation effect of confirmation PU/PVDF electrospinning film can promote the functions (referring to accompanying drawing 7-12) such as migration, adhesion and extracellular matrix secretion of HSF respectively.
Embodiment 7 animals accelerate the effect of wound healing as functional wound dressing at body laboratory observation PU/PVDF electrospinning film
(1) the PU/PVDF electrospinning film that embodiment 3 is prepared soaks 10 minutes in 70% alcoholic solution after, soaked 30 minutes with normal saline again, to reach the purpose of solvent composition that may be residual in sterilization and the thorough removal PU/PVDF electrospinning film;
(2) each PU/PVDF electrospinning film is respectively applied to the skin of back wound repair (excision diameter be the circular holostrome skin of 1 cm) of SD rat, Continuous Observation wound healing situation.Compare with traditional gauze class dressing, use the wound of functional wound dressing of the present invention to restore haply in the 21st day, the treatment period interval was changed dressings 1 time in 2 days, and used the wound of normal gauze class dressing roughly to restore in the 28th day ability, changed dressings every other day during the treatment 1 time.Simultaneously, functional wound dressing of the present invention and wound surface is less sticks together is difficult for hemorrhagely when changing dressings, and the dressing of normal gauze class is normal and wound surface adhesion, hemorrhage.
Claims (3)
1. functional wound dressing that can accelerate wound healing is characterized in that: described functional wound dressing is a kind of polyurethane/polyvinylidene fluoride electrospinning film, and wherein the mass ratio of polyurethane and polyvinylidene fluoride is 1:3 ~ 3:1; The thickness of described polyurethane/polyvinylidene fluoride electrospinning film is 150 ~ 250 μ m.
2. the preparation method that can accelerate the functional wound dressing of wound healing is characterized in that, comprises the steps:
(1) polyurethane and polyvinylidene fluoride are dissolved in respectively in the mixed solvent of oxolane and dimethyl formamide and are mixed with polyurethane spinning liquid and polyvinylidene fluoride spinning liquid; The volume ratio of oxolane and dimethyl formamide is 1:1 in the described mixed solvent; The mass volume ratio of solute and solvent is 12% in described polyurethane spinning liquid and the polyvinylidene fluoride spinning liquid;
(2) polyurethane spinning liquid and the polyvinylidene fluoride spinning liquid with preparation in the step (1) be 1:3 ~ 3:1 mixing by volume, is mixed with the polyurethane of different proportion composition/polyvinylidene fluoride blend spinning liquid;
(3) polyurethane of the different proportion composition of preparation in the step (2)/polyvinylidene fluoride blend spinning liquid is obtained polyurethane/polyvinylidene fluoride electrospinning film by electrostatic spinning respectively;
(4) the middle polyurethane for preparing of step (3)/polyvinylidene fluoride electrospinning film was soaked 10 minutes in 70% alcoholic solution first, soaked 30 minutes with normal saline again, namely get the functional wound dressing that can accelerate wound healing.
3. preparation method according to claim 2, it is characterized in that: the syringe pump flow velocity is 0.8 ~ 1.0 ml/h during the middle electrostatic spinning of described step (3), the high voltage direct current generator output voltage is 12 ~ 18kV, and the distance between syringe pump syringe needle and the dash receiver is 15 ~ 20 cm.
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| CN107737364A (en) * | 2017-11-23 | 2018-02-27 | 广州市众为生物技术有限公司 | A kind of wound dressing and preparation method thereof |
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| CN113026198B (en) * | 2019-12-09 | 2022-07-08 | 中昊晨光化工研究院有限公司 | Functional dressing and preparation method thereof |
| CN113274539A (en) * | 2021-04-29 | 2021-08-20 | 西安理工大学 | Self-powered wound patch and preparation method thereof |
| CN113304303A (en) * | 2021-05-18 | 2021-08-27 | 南通大学 | Micro-current elastic dressing for chronic wound healing and preparation method thereof |
| CN115387029A (en) * | 2022-08-23 | 2022-11-25 | 西安理工大学 | Preparation method of stripe structure self-powered adhesive bandage |
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