CN102860227A - Test device and method used for studying root phenotypic plasticity - Google Patents

Abstract

The invention relates to a test device and method for studying root phenotype plasticity. The test device includes a square tube for sowing plant seeds, and is characterized in that: the square tube is provided with a vertical partition layer, to separate two support areas for placing nutrient patches of different nutrients, the bottom of the square tube is provided with a bottom plate, and the square tube and the vertical compartment are made of light-transmitting and impermeable hard materials In order to block the interpenetration of roots and the flow of nutrients in different support areas, a test window is provided on the side wall of the square tube, and a seeding trough is provided on the upper side of the middle part of the vertical compartment. This experimental device can study the ability of local roots of plants to sense environmental changes and initiate adaptation strategies after the soil nutrients are exhausted over time in the state closest to nature, especially the ability to test the plasticity of plant root phenotypes. Its overall structure is simple, its cost is low, and its materials and production are convenient.

Description

An experimental device and method for studying root phenotypic plasticity

technical field

The invention relates to the research field of plants adapting to soil nutrient spatial heterogeneity, and mainly relates to the research on the phenotype plasticity of plant roots. the

Background technique

Soil available nutrient content is one of the important attributes of ecosystems, and the spatial heterogeneity of soil nutrients generally exists in natural ecosystems. The spatial scale of soil available nutrient content varies greatly, which can reach the range perceived by individual plant roots. In the long-term evolution process, in order to maximize the acquisition of soil resources, plants have various plastic responses to the spatial heterogeneity of nutrients, including morphological plasticity, physiological plasticity, and mycorrhizal plasticity. The roots of many plant species proliferate in nutrient-rich patches, and the degree of proliferation varies greatly among species and is affected by patch attributes (patch size, nutrient concentration), nutrient element types, and overall nutrient supply status. Plants also adjusted the diameter, branch angle, internode spacing and spatial configuration of fine roots in nutrient-rich patches to achieve efficient use of nutrients in the patch. the

Contents of the invention

The invention provides a test device and method for studying the plasticity of root system phenotype. The test device can be used to study the changes in the root system's perception of the environment after the soil nutrients are exhausted over time in the local root system of a plant in the state closest to nature. And the ability to initiate adaptation strategies, especially the ability to experiment with plant root phenotypic plasticity. the

The technical solution of the present invention is: a test device for studying root phenotype plasticity, including a square tube for sowing plant seeds, characterized in that: the square tube is provided with a vertical partition along the vertical direction of the center , to separate two feeding areas for placing nutrient patches of different nutrients, the bottom of the square tube is provided with a bottom plate, and the square tube and the vertical compartment are made of light-transmitting and water-proof hard materials , to block the interpenetration of roots and the flow of nutrients in different support areas, a test window is provided on the side wall of the square tube, and a seeding trough is provided on the upper side of the middle part of the vertical compartment. the

In one embodiment, the test window is located at the center of each feeding area, and the test window is covered with tin foil to prevent water loss. the

In one embodiment, the hard material is glass. the

Another technical scheme of the present invention is: a kind of test method for studying root system phenotype plasticity, comprises above-mentioned test device, it is characterized in that, carry out according to the following steps:

(1) Take a certain number of high-quality plant seeds, sow the plant seeds in the seeding trough of the above-mentioned test device, and place corresponding nutrient patches with different nutrients in the feeding area of each test device;

(2) Divide the above-mentioned test devices with plant seeds and nutrient patches into several batches;

(3) After regularly measuring the water potential in different supporting areas of each test device through the test window, seal the test window with tin foil to prevent water loss;

(4) Take out a batch of test devices regularly for observation: Take the root system in each feeding area to measure the morphological indicators.

(5) Record the test data: the distribution and growth of the root system in each supporting area at different time periods. the

In the above step (3), after measuring the water potential of each feeding area through the square cylinder test window with a water potential measuring instrument, replenish water or nutrient solution in time until different feeding areas have the same water potential. the

In the above step (4), the root system of each supporting area is taken, the image of each root system is scanned by a digital scanner, and the root system growing in each supporting area is quantitatively analyzed by image analysis software. the

The advantage of the present invention is that: the test device of the present invention can study the ability of the local roots of plants to sense environmental changes and initiate adaptation strategies after the soil nutrients are exhausted over time in the state closest to nature, especially for test plants. Root capacity for phenotypic plasticity. Its overall structure is simple, its cost is low, and its materials and production are convenient. the

Description of drawings

Fig. 1 is a schematic structural view of the test device of the present invention. the

In the figure: 1—vertical compartment, 2—support area, 3—test window, 4—side wall of square tube, 5—bottom plate of square tube, 6—sowing trough. the

Detailed ways

In order to describe the technical content, structural features, achieved goals and effects of the present invention in detail, the following will be described in detail in conjunction with the embodiments and accompanying drawings. the

As shown in Figure 1, a schematic structural view of the test device of the present invention, one side of the figure is shown in perspective to fully demonstrate the shelf structure of the present invention, and the other side is a schematic diagram of the shape. the

As shown in Fig. 1, a kind of experimental device for studying the plasticity of root system phenotype comprises a square tube for sowing plant seeds and a vertical compartment nested in the middle of the square tube, and the square tube is provided with a vertical wall along the center vertical direction. Straight interlayer 1, to separate two support areas 2 for placing nutrient patches of different nutrients, said vertical interlayer 1 central position is provided with a sowing groove 6 for sowing the seeds of test plants, said square tube A test window 3 leading to each supporting area is evenly distributed on the two walls parallel to the vertical compartment, and the side wall 4 of the square tube, the bottom plate 5 of the square tube and the vertical compartment 1 are all light-transmitting and impervious to water. Materials (such as glass) to block root interpenetration and nutrient flow in different feeding areas. the

In this embodiment, the vertical partition is arranged on the central symmetrical plane of the square tube, and the square tube and the vertical partition constitute two equal-shaped feeding areas. the

In this embodiment, the test window (to measure the water potential to ensure that the water potential of the two feeding areas is the same) is set at the center of each feeding area, and the testing window is covered with tin foil to prevent water loss. the

A test method for studying root phenotype plasticity, comprising the above-mentioned test device, is carried out in the following steps:

(1) Take a certain number of high-quality plant seeds, sow the plant seeds in the seeding trough of the above-mentioned test device, and place corresponding nutrient patches with different nutrients in the feeding area of each test device;

(2) Divide the above-mentioned test devices with plant seeds and nutrient patches into several batches;

(3) After regularly measuring the water potential in different supporting areas of each test device through the test window, seal the test window with tin foil to prevent water loss;

(4) Take out a batch of test devices regularly for observation: Take the root system in each feeding area to measure the morphological indicators.

(5) Record the test data: the distribution and growth of the root system in each supporting area at different time periods. the

In the above step (3), in order to ensure that each feeding area has the same water potential, use a water potential measuring instrument to measure the water potential of each feeding area through the test window of the square tube, and then replenish water or nutrient solution to the feeding area in time until different feeding areas with the same water potential. the

The above step (4) is to take the root system of each supporting area, scan the image of each root system through a digital scanner, and apply image analysis software to perform quantitative analysis on the root system growing in each supporting area. the

A more specific implementation is as follows:

To study the phenotypic plasticity of Chinese fir root system in a heterogeneous phosphorus supply environment: the side wall, bottom plate and interlayer of the square tube are made of 2 mm thick glass material, and the joints of the square tube are required to be tight and watertight. In the seeding trough of the interlayer (non-treatment area), the root system can grow freely to the treatment area after the seeds germinate and take root. When soil heterogeneous phosphorus treatment is carried out, patches of granular long-acting slow-release phosphorus sources are applied to each feeding area (treatment area). The sand culture substrate is vanadium (Al ₂ O ₃ , 28-48 mesh) mixed with quartz sand at a ratio of 1.5%. The bauxite needs to be dephosphorized before potting, and the bauxite should be washed with clean water until the water is dry If it is turbid, soak it with 0.01mol/L HCl for 24 hours, then rinse the acid on the surface of the alumina with clean water, soak it with 0.01mol/L HCl for 24 hours, and repeat the treatment 10 times to ensure that the phosphorus in the alumina is fully eluted , air-dried for later use. Quartz sand should also be washed with water to ensure that its phosphorus is fully eluted. The matrix used in the phosphorus source patch was soaked with different concentrations of KH ₂ PO ₄ mixed sandy soil, using the characteristics of vanadium soil that can effectively absorb phosphorus and release available phosphorus slowly and evenly, to control the supply of available phosphorus by the cultivation medium, the KH ₂ applied PO ₄ was first dissolved in distilled water, mixed with bauxite and soaked for 24 hours, then added quantitative dry sand and mixed evenly. During the test period, normal nutrient solution and water supply except for phosphorus were carried out. After measuring the water potential of each feeding area through the square tube test window with a water potential meter, water or nutrient solution was added to the feeding area in time until different feeding areas With the same water potential, use tin foil to cover the test window after the water potential measurement to prevent water loss. After the test treatment time is over, the device is divided into plaques, and the root system of each feeding area is taken, and the images of each root system are scanned by a digital scanner, and the root system of each layer is quantitatively analyzed by image analysis software.

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the description of the present invention and the contents of the accompanying drawings, or directly or indirectly used in other related All technical fields are equally included in the scope of patent protection of the present invention. the

Claims (6)

1. experimental rig that is used for research root system phenotypic plasticity, comprise the square tube for the sowing plant seed, it is characterized in that: described square tube is provided with 1 vertical interlayer along the central vertical direction, to be separated out 2 keeping zones that are used for the nutrient patch of placement Different Nutrients, the bottom of described square tube is provided with base plate, described square tube and vertical interlayer all adopt the fluid-tight hard material of printing opacity to make, to intercept different the flowing of the interior root system IPN in zone and nutrient of supporting, offer testing window on the described square tube sidewall, the middle part upside of described vertical interlayer is provided with planting tank.

2. experimental rig according to claim 1 is characterized in that: described testing window is positioned at each center of supporting the zone, hides tinfoil on the described testing window, with the loss that prevents that water from dividing.

3. experimental rig according to claim 1, it is characterized in that: described hard material is glass.

4. a test method that is used for research root system phenotypic plasticity comprises such as each described experimental rig among the claim 1-3, it is characterized in that, carries out according to the following steps:

(1) get the colory plant seed of some, plant seed be seeded in the planting tank of above-mentioned experimental rig, and in the keeping zone of each experimental rig the corresponding nutrient patch that is placed with Different Nutrients;

(2) the above-mentioned experimental rig that is placed with plant seed and nutrient patch is divided into some batches;

(3) regularly measure the different flows of water of supporting the zone of each experimental rig by testing window after, adopt tinfoil that testing window is sealed, with the loss that prevents that water from dividing;

(4) regularly taking out a collection of experimental rig observes: get each and support the interior root system in zone to carry out the mensuration of morphological indexes;

(5) record test data: each supports distribution and the growing state of root system in the zone different time sections.

5. test method according to claim 4, it is characterized in that: in above-mentioned steps (3), after utilizing Type Water Potential Meter to measure each flow of water of supporting the zone by described square tube testing window, in time keep the skin wet or nutrient solution to supporting in the zone, until the different identical flow of water of regional tool of supporting.

6. test method according to claim 4, it is characterized in that: in above-mentioned steps (4), get the root system that each supports the zone, scan each root system image by the digital scanning instrument, applies image analysis software supports the root system of region growing to carry out quantitative analysis to each.

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