CN102851320A - Fermentation method for producing ethanol by immobilized yeast - Google Patents
Fermentation method for producing ethanol by immobilized yeast Download PDFInfo
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- CN102851320A CN102851320A CN2012103045471A CN201210304547A CN102851320A CN 102851320 A CN102851320 A CN 102851320A CN 2012103045471 A CN2012103045471 A CN 2012103045471A CN 201210304547 A CN201210304547 A CN 201210304547A CN 102851320 A CN102851320 A CN 102851320A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
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Abstract
The invention provides a fermentation method for producing ethanol by immobilized yeast. According to the method, porous foam ceramic is used as an immobilized carrier, and sugarcane juice is used a medium.
Description
Technical field
The invention provides a kind of method of utilizing the Immobilized yeast producing and ethanol, utilize foam porous pottery to be fixation support, take sugar cane juice as substratum, the method for Immobilized yeast producing and ethanol.
Background technology
Along with being becoming tight the day of petroleum resources in the world and the continuous increase of pollution emission reduction pressure, the clean energy take alcohol fuel as representative is paid close attention to widely, and wherein one of focus of research is alcohol fuel.Alcohol fuel is a kind of reproducibility energy that can replace oil to be used as fuel, also is a kind of important basic chemical raw materials.After 20 century 70 oil crisises, along with the increase of people to the renewable resources attention degree, the exploitation of alcohol fuel receives more concern, makes its suitability for industrialized production face unprecedented opportunity to develop.Biomass resource has the characteristics such as large, the renewable and abundant species of turnout is various, is one of the energy of most potential alternative oil and raw material.People are positive Looking For Substitutions Of Oil one alcohol fuel, and the alcohol fuel comprehensive benefit of utilizing that acts as a fuel excites people that its production cost and efficient are studied, to obtaining high-efficiency fermenting bacterial strain, low-cost substrate and optimal conditions of fermentation.
The alcohol fuel as an alternative green regenerative energy sources of petroleum-based energy causes extensive concern in the whole world, is rapidly developed in states such as American-European and Brazil.On the other hand, ceramic immobilization technology is that the cell that will live is intensive on pottery and constantly breeding, forms the high-density biology catalyzer, and then can increase substantially the microorganism concn of participating in reaction, improves catalytic efficiency; And the shortcoming such as the stability, the physical strength that have overcome traditional immobilization carrier be low.
Summary of the invention
The invention provides a kind of method of utilizing the Immobilized yeast producing and ethanol, utilize foam porous pottery to be fixation support, take sugar cane juice as substratum, the method for Immobilized yeast producing and ethanol.
1. method of utilizing the Immobilized yeast producing and ethanol places foam porous pottery that 100mL concentration is housed is 10
8In the high pin beaker of individual/mL sterilised yeast suspension, under 20 ℃, shaking table frequency 120r/min, absorption 90min takes out and puts into baking oven, in 40 ℃ of lower baking 10 h.Get pottery behind the immobilized yeast and move into and be equipped with in the high pin beaker of 100mL sugarcane juice substratum, carry out static fermentation, measure the fermented liquid alcoholic strength behind 54 h.
2. the described sugarcane juice substratum of step 1 is sugar cane crushing, filters, and regulates pH value to 4.4, sterilization.
3. the described bacteria suspension of step 1 is will cultivate ripe yeast under aseptic condition, inoculation l encircles in the liquid seed culture medium in the Erlenmeyer flask, 150 r/min, 28 ℃ cultivate centrifugal 10min (3000 r/min) abandoning supernatant behind the 24h, prepare bacteria suspension (0.2g wet thallus/mL), place 4 ℃ of refrigerators for subsequent use with behind the stroke-physiological saline solution washing thalline 3 times with sterilized water.
4. the described optimum fermentation temp of step 1 is 29 ℃.
5. the described best fermentation pH value of step 1 is 5.5.
6. the described best sugar hammer degree of step 1 is 18 ° of Bx.
Beneficial effect of the present invention is: the present invention studies the fermentation by saccharomyces cerevisiae performance that is fit to ethanol fermentation, and take foam porous pottery as fixation support, immobilization fermentation technique is optimized, produces alcohol fuel for ceramic Immobilized yeast sugarcane juice scientific basis is provided.
Description of drawings
Fig. 1 differing temps is on the impact of ethanol fermentation.
The different pH values of Fig. 2 are on the impact of ethanol fermentation.
Fig. 3 different culture media sugar hammer degree is on the impact of ethanol production.
Embodiment
The following examples elaborate to the present invention, but to not restriction of the present invention.
The used bacterial strain of this patent be yeast saccharomyces cerevisiae (
Saccharomyces cerevisiae) CICC 31077, be preserved in Chinese industrial microbial strains preservation administrative center.
Embodiment 1
The implementation case explanation different fermentations temperature for inquiring into the different fermentations temperature to the impact of ethanol production, uses the different fermentations temperature to carry out ethanol fermentation to the impact of ethanol fermentation, and the result as shown in Figure 1.
In pH value 4.5, static fermentation 54h under 18 ° of Bx conditions of sugar hammer degree, obtaining temperature affects graph of a relation to alcohol concn.As shown in Figure 1, along with temperature raises, the rear reduction that raises first of final fermented liquid ethanol content reaches maximum in the time of 29 ℃.Aspect fermentation rate, along with temperature raises, fermentation rate raises gradually, and in the time of 32 ℃, yeast fermentation speed will be a little more than 29 ℃.As can be known in this temperature range, along with the breeding of temperature rising yeast growth is faster; If but excess Temperature, then yeast growth is too fast, and the thalline number is too much, makes aerobic repiration consume more multiple ferment substrate, is unfavorable on the contrary the accumulation of final product.Comprehensive fermentation rate and ethanol ultimate density, leavening temperature is conducive to ethanol fermentation most in the time of 29 ℃.
The implementation case illustrates different initial pH value to the impact of ethanol production, for inquiring into different initial pH value to the impact of ethanol production, uses different initial pH value to carry out ethanol fermentation, and the result as shown in Figure 2.
29 ℃ of temperature, static fermentation 54 h under 18 ° of Bx conditions of sugar hammer degree, obtaining the pH value affects graph of a relation to alcohol concn, and as shown in Figure 2, initial pH value was at 5.5 o'clock, and final alcohol concn is the highest.But can find out from fermenting process, the pH value is 5.0 o'clock, in earlier stage (0 ~ 27 h) fermentation rate is apparently higher than pH value 5.5, alcohol concn is also the highest, and stage slows down relatively.Causing this phenomenon reason may be can produce a large amount of CO in the ethanol fermentation process
2Fermented liquid pH value is reduced, make the fermentation later stage, more be conducive to yeast fermentation ethanol during pH value 5.5, because final fermented liquid alcohol concn is higher.The suitable yeast sugarcane juice ethanol fermentation of pH value 5.5 that hence one can see that.
Embodiment 3
The implementation case explanation different culture media sugar hammer degree for inquiring into different culture media sugar hammer degree to the impact of ethanol production, uses different culture media sugar hammer degree to carry out ethanol fermentation to the impact of ethanol production, and the result as shown in Figure 3.
At 29 ℃, static fermentation 54h under pH value 5.5 conditions, obtaining sugar hammer degree affects graph of a relation to alcohol concn, as shown in Figure 3.Along with substratum hammer degree raises, ethanol content is larger.When the hammer degree was 22 ° of Bx, alcohol concn was the highest.But when the hammer degree was 22 ° of Bx, alcohol concn was slightly high during only than 18 ° of Bx, and fermentation rate is lower than 18 ° of Bx.Because the original hammer degree of fermented liquid sugarcane juice is 18 ° of Bx, if improve fermentation liquid hammer degree, need fermented liquid is concentrated, thereby will increase energy consumption, greatly increase fermentation costs, but alcohol concn improves seldom.In general, when sugar hammer degree is 18 ° of Bx, be fit to the sugarcane juice ethanol fermentation.
Claims (6)
1. method of utilizing the Immobilized yeast producing and ethanol places foam porous pottery that 100mL concentration is housed is 10
8In the high pin beaker of individual/mL sterilised yeast suspension, under 20 ℃, shaking table frequency 120r/min, absorption 90min takes out and puts into baking oven, in 40 ℃ of lower baking 10 h; Get pottery behind the immobilized yeast and move into and be equipped with in the high pin beaker of 100mL sugarcane juice substratum, carry out static fermentation, measure the fermented liquid alcoholic strength behind 54 h.
2. method according to claim 1 is characterized in that, the sugarcane juice substratum is sugar cane crushing, filters, and regulates pH value to 4.4, sterilization.
3. method according to claim 1, it is characterized in that, bacteria suspension is will cultivate ripe yeast under aseptic condition, inoculation l encircles in the liquid seed culture medium in the Erlenmeyer flask, 150 r/min, 28 ℃ cultivate centrifugal 10min abandoning supernatant behind the 24h, prepare bacteria suspension with behind the stroke-physiological saline solution washing thalline 3 times with sterilized water, place 4 ℃ of refrigerators for subsequent use.
4. method according to claim 1 is characterized in that, leavening temperature is 29 ℃.
5. method according to claim 1 is characterized in that, fermentation pH value is 5.5.
6. method according to claim 1 is characterized in that, sugar hammer degree is 18 ° of Bx.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107048472A (en) * | 2017-05-26 | 2017-08-18 | 河南中烟工业有限责任公司 | Porous ceramics immobilization pectase and its application in reconstituted tobacoo process |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1888062A (en) * | 2006-07-21 | 2007-01-03 | 北京化工大学 | Yeast cell immobilizing method |
| CN101225407A (en) * | 2007-12-14 | 2008-07-23 | 华南理工大学 | A method for producing alcohol by fermenting immobilized yeast with sugarcane as raw material |
| CN101434911A (en) * | 2008-10-06 | 2009-05-20 | 广西科学院 | Wine brewing yeast strain and method for producing ethanol by efficient sugarcane juice fermentation |
| CN101597555A (en) * | 2009-07-07 | 2009-12-09 | 广州甘蔗糖业研究所 | The manufacture method of rum |
| CN101633937A (en) * | 2009-08-20 | 2010-01-27 | 华南理工大学 | Alcohol preparation method by fermenting cane juice with foamed ceramic immobilized yeast |
| CN101979615A (en) * | 2010-11-25 | 2011-02-23 | 南京工业大学 | A method for the continuous production of bio-butanol by separation and coupling of fixed-bed fermentation |
| CN102066923A (en) * | 2008-04-25 | 2011-05-18 | E-燃料公司 | Micro refinery system for ethanol production |
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2012
- 2012-08-24 CN CN2012103045471A patent/CN102851320A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1888062A (en) * | 2006-07-21 | 2007-01-03 | 北京化工大学 | Yeast cell immobilizing method |
| CN101225407A (en) * | 2007-12-14 | 2008-07-23 | 华南理工大学 | A method for producing alcohol by fermenting immobilized yeast with sugarcane as raw material |
| CN102066923A (en) * | 2008-04-25 | 2011-05-18 | E-燃料公司 | Micro refinery system for ethanol production |
| CN101434911A (en) * | 2008-10-06 | 2009-05-20 | 广西科学院 | Wine brewing yeast strain and method for producing ethanol by efficient sugarcane juice fermentation |
| CN101597555A (en) * | 2009-07-07 | 2009-12-09 | 广州甘蔗糖业研究所 | The manufacture method of rum |
| CN101633937A (en) * | 2009-08-20 | 2010-01-27 | 华南理工大学 | Alcohol preparation method by fermenting cane juice with foamed ceramic immobilized yeast |
| CN101979615A (en) * | 2010-11-25 | 2011-02-23 | 南京工业大学 | A method for the continuous production of bio-butanol by separation and coupling of fixed-bed fermentation |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107048472A (en) * | 2017-05-26 | 2017-08-18 | 河南中烟工业有限责任公司 | Porous ceramics immobilization pectase and its application in reconstituted tobacoo process |
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Application publication date: 20130102 |