CN102813669A - Application of Isaria felina crude polysaccharide to preparation of anti-tumor drug - Google Patents
Application of Isaria felina crude polysaccharide to preparation of anti-tumor drug Download PDFInfo
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Abstract
The invention relates to the development of fungi extract as anti-tumor drugs, specifically relates to application of an Isaria felina crude polysaccharide to preparation of anti-tumor drugs, and solves a problem that anti-tumor active component in Isaria felina has not been reported. The invention adopts Kunming mice and nude mice for experiments. The Kunming mice are respectively inoculated with mouse H22 hepatoma cells, mouse S180 sarcoma cells and mouse EAC ascites carcinoma cells, and then are injected with IFP of various doses. The experimental result shows that the IFP has good inhibitory effect on H22 transplanted tumor and S180 transplanted tumor, and life prolonging effect on ascites carcinoma EAC mice. The nude mice are respectively inoculated with human HepG2 hepatocellular carcinoma cells and human Hela cervical carcinoma cells, and then injected with IFP of various doses. The experimental result shows that the IFP has good inhibitory effect on HepG2 transplanted tumor and Hela transplanted tumor. Therefore, the IFP has significant effect in inhibiting tumor, and can be widely applied to the preparation of antitumor drugs.
Description
Technical field
The present invention relates to the antitumor drug exploitation of fungal extract, be specially the application of a kind of cat Isaria crude polysaccharides (hereinafter to be referred as IFP) as the preparation antitumor drug.
Background technology
The cat Isaria (
Isaria felina) be from the natural cordyceps sporophore, to cultivate a kind of bacterial strain that obtains through strain separating, in classification, belong to deuteromycetes, Moniliales, Isaria genus.Be accredited as through Institute of Microorganism, Academia Sinica
Isaria felina(DC.:Fr.) Fr., this strain are by China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation (preserving number: CGMCC NO. 0706), and obtain national inventing patent (ZL02103669.1).People such as Deffieux Gerard (J Antibiot (Tokyo). 1981,34 (10): 1261-1265.) from
Isaria felinaIn extraction separation obtain insecticide Isariins B, C and the D of cyclodepsipeptide structure, and to the structure of Isariins B, C and D carried out identifying (J Antibiot (Tokyo). 1981,34 (10): 1266-1270.).Guo Yongxia separation and purification from cat Isaria mycelium obtains having the cyclodepsipeptide isarfelin of antifungic action; And analysis, structure that isarfelin has carried out physicochemical property identified; And (Guo Yongxia is about the separation and purification and the The Characteristic Study of cat Isaria bacterium anti-fungus polypeptide and huge Tricholoma mongolicum Imai EAFP: [thesis for the doctorate] Beijing to find to have the activity that suppresses antibacterial, opposing fungus by isarfelin; China Agricultural University, 2005).Ikumoto etc. discover that the leachate of Cordyceps, Cordyceps militaris (L.) Link. and cat Isaria has negative blockage effect to isolated atria; Simultaneously to condensing of the contractile response of electricity irritation ileum and human blood platelets inhibitory action is arranged (Journal of the Pharmaceutical Society of Japan; 1991,111 (9): 504-509).Patent ZL02103669.1 discloses the anti-tumor activity function of cat Isaria, but not having any research at present finds its anti-tumor active ingredient from the cat Isaria.
Summary of the invention
The present invention provides the antineoplastic component in the cat Isaria in order to solve the problem of not finding anti-tumor active ingredient in the cat Isaria at present, and promptly cat Isaria crude polysaccharides is as the application of preparation antitumor drug.
Cat Isaria crude polysaccharides, it is made by following steps: get mycelium powder, add the distilled water of 3-5 times of mycelium powder weight, boiling water bath extracts 70-110min, centrifugal 4-6min under the 3000r/min rotating speed; In deposition, continue to add the distilled water of 3-5 times of mycelium powder weight, boiling water bath extracts 20-40min, centrifugal 4-6min under the 3000r/min rotating speed; In deposition, continue to add the distilled water of 3-5 times of mycelium powder weight, boiling water bath extracts 20-40min, the centrifugal 4-6min of 3000r/min; Merge the supernatant of three extractions, water-bath is concentrated into 800-1000mL, gets mixed liquor; 95% ethanol (be preferably mixeding liquid volume 3.75 times) that adds 3-4 times of mixeding liquid volume is mixing fully, and deposition is spent the night; Get deposition, in 70 ℃ dry cat Isaria crude polysaccharides.The above-mentioned cat Isaria crude polysaccharides that makes can be applicable to prepare in the antitumor drug.
The present invention adopts SPF level kunming mice, SPF level nude mice to carry out zoopery; Kunming mice is inoculated the IFP that injects multiple dosage behind mice H22 HCC, mice S180 sarcoma cell, the mice EAC ascites cells respectively respectively and injected distilled water (negative control group), mitomycin for inj, Oleum Fructus Bruceae oral latex emulsion as matched group; Experimental result shows that IFP compares with negative control group and has significant difference, explains that cat Isaria crude polysaccharides has the good restraining effect and ehrlich ascites carcinoma EAC mice is had life prolongation effect H22 transplanted tumor, S180 transplanted tumor; Nude mice is inoculated the IFP that injects multiple dosage behind people HepG2 HCC, the people Hela cervical cancer cell respectively respectively and injects distilled water (negative control group), mitomycin for inj, Oleum Fructus Bruceae oral latex emulsion as matched group; Experimental result shows that IFP compares with negative control group and has significant difference, explains that cat Isaria crude polysaccharides has the good restraining effect to HepG2 transplanted tumor, Hela transplanted tumor.In sum, IFP has the effect of significant inhibition tumor, can be in the preparation antitumor drug.
The specific embodiment
Embodiment 1: down in the face of the research that experimentizes of the antihepatocarcinoma effect of cat Isaria crude polysaccharides:
1. material and method
1.1 animal
54 of SPF (specific pathogen free) level kunming mices, body weight 20-22g, male, by Shanxi Tumors Inst.'s Animal Lab. breeding, laboratory animal production licence number: SCXK (Shanxi) 2007-0001; Laboratory animal occupancy permit number: SYXK (Shanxi) 2007-0002.
54 of SPF level BALB/c-nu nude mices, body weight 16-18g, male, available from Beijing Vital River Experimental Animals Technology Co., Ltd., animal production licence number: SCXK (capital) 2009-0017; Experimental animal feeding is at cleaning level barrier environment Animal Lab., laboratory animal occupancy permit SYXK (Shanxi) 2007-0002.
Medicine and reagent
The cat Isaria is from the natural cordyceps sporophore, after strain separating, to cultivate to obtain, and its cultural method is following: with 500 ml culture medium (peptone 0.6 %, yeast extract 1.2 %, sucrose 2.4 %, MgSO
40.05 % uses 0.1 %KH
2PO
4Adjustment pH is 6.5-7.0) be loaded in the 3000mL triangular flask, kraft paper seals, and 125 ℃ of autoclaving 40min are cooled to room temperature, insert strain, place 180rmin
-1On the shaking table, transmit ferment in 23-27 ℃ of condition underspin and cultivate 72h, collect fermentation liquid, with fermentation liquid in 2000rmin
-1Centrifugal 5min collects mycelium, and mycelium is pulverized after 80 ℃ of oven dry, crosses 100 mesh sieves.
Cat Isaria crude polysaccharides, it is made by following steps: get the above-mentioned mycelium powder of 300g, add the 1200ml distilled water, boiling water bath extracts 90min, the centrifugal 5min of 3000r/min; In deposition, continue to add the 1200ml distilled water, boiling water bath extracts 30min, the centrifugal 5min of 3000r/min; In deposition, continue to add the 1200ml distilled water, boiling water bath extracts 30min, the centrifugal 5min of 3000r/min; Merge the supernatant of three extractions, water-bath is concentrated into 900mL, gets mixed liquor; 95% ethanol that adds 3.75 times of mixeding liquid volumes, abundant mixing, deposition is spent the night; Get deposition, in 70 ℃ of dry for standby.
The tumor strain: mice H22 HCC, purchase Experimental Animal Center in Hebei Medical University; People HepG2 HCC is presented by University Of Shanxi's biotechnology research.
Mitomycin for inj: Western medicine cancer therapy drug commonly used, to produce by Haizheng Medicine Stock Co., Ltd., Zhejiang Prov, batch number is 110601.
The Oleum Fructus Bruceae oral latex emulsion: Chinese medicine cancer therapy drug commonly used, to produce by the big Pharmaceutical of Shenyang medicine Co., Ltd, product batch number is 1103112.
Experimental technique:
With well-grown mice H22 liver cancer tissue tumor piece, under aseptic condition, add normal saline, using disinfectant glass grinding device to process concentration is 1 * 10
7The cell suspension of/mL, the right oxter that is inoculated in all kunming mices after the filtration respectively is subcutaneous, every kunming mice inoculation 0.2mL tumor liquid.
With well-grown people HepG2 liver cancer tissue tumor piece, under aseptic condition, add normal saline, using disinfectant glass grinding device to process concentration is 5 * 10
6The cell suspension of/mL, the right oxter that is inoculated in all nude mices after the filtration respectively is subcutaneous, every nude inoculation 0.2mL tumor liquid.
Behind kunming mice inoculation mice H22 HCC 24 h, it is divided into 6 groups at random, is respectively dose groups, IFP low dose group among negative control group, mitomycin group, Fructus Bruceae line of oils, IFP high dose group, the IFP, every group of 9 animals.The negative control group mice is irritated stomach and give distilled water 0.2mL every day; Fructus Bruceae line of oils mice is irritated stomach and give Oleum Fructus Bruceae oral latex emulsion 8mL/kg every day; IFP high dose group mice is irritated stomach and give IFP 200 mg/kg every day; The dose groups mice is irritated stomach and give IFP 100 mg/kg every day among the IFP, and IFP low dose group mice is irritated stomach and give IFP 50 mg/kg every day; Mitomycin group mouse peritoneal injection mitomycin 1 mg/kg respectively at experiment 2d, each injection of 6d once, injects 2 times altogether.Gastric infusion 10d, mice body weight of weighing in per 3 days, the cervical vertebra dislocation method is put to death mice behind last administration 24 h, cuts open and gets the tumor piece and weigh heavy, the tumour inhibiting rate (%) of calculating tumor.
Behind nude inoculation people HepG2 HCC 24 h, also it is divided into 6 groups at random, is respectively dose groups, IFP low dose group among negative control group, mitomycin group, Fructus Bruceae line of oils, IFP high dose group, the IFP, every group of 9 animals.Gastric infusion dosage is the same, continuous gastric infusion 20d, and laboratory observation 50d weighs and puts to death animal in experiment 51d, peels off the tumor piece and claims weight in wet base, calculates that tumor is heavy, tumour inhibiting rate (%).
Data processing method
Adopt the SPSS17.0 statistical software to carry out data statistic analysis, adopt one factor analysis of variance (ANOVA), relatively adopt least significant difference method (LSD) between two groups, experimental data with
Expression,
P<0.05 expression has significant difference.
Experimental result
2.1 IFP is to the inhibitory action of mouse bearing liver cancer H22
Following table 1 is respectively to organize heavy, the tumour inhibiting rate of tumor of kunming mice:
Table 1 IFP is to the inhibitory action of mouse bearing liver cancer H22
Annotate: * and negative control group are relatively
P<0.05
Experimental result shows, compares with negative control group, and mitomycin and IFP have the good restraining effect to H22 transplanted tumor, and wherein the tumor killing effect of mitomycin is best, and inhibitory rate is to 61.27%, compares with negative control group to have significant difference; IFP also has the good restraining effect to H22 transplanted tumor; Compare with negative control group and to have significant difference; The IFP high dose group inhibitory rate of 200mg/kg to 47.40% and among the IFP of 100mg/kg the IFP low dose group tumor killing effect of dose groups and 50mg/kg be respectively 31.21% and 9.83%; All demonstrate certain tumor killing effect, IFP is described simultaneously to the increase along with dosage of the inhibitory action of H22 transplanted tumor, its tumour inhibiting rate increases gradually; It is 12.72% that Oleum Fructus Bruceae also has certain tumor killing effect to H22 transplanted tumor.
2.2 IFP is to the inhibitory action of nude mice transplanted hepatoma HepG2
Following table 2 is respectively to organize heavy, the tumour inhibiting rate of tumor of nude mice:
Table 2 IFP is to the inhibitory action of nude mice transplanted hepatoma HepG2
Annotate: * and negative control group are relatively
P<0.05
Experimental result shows that compare with negative control group, mitomycin and IFP have the good restraining effect to HepG2 transplanted tumor, and wherein the tumor killing effect of mitomycin is best, has reached 54.73%, compares with negative control group to have significant difference; IFP also has the good restraining effect to HepG2 transplanted tumor; Compare with negative control group and to have significant difference; The IFP high dose group inhibitory rate of 200mg/kg to 29.73% and among the IFP of 100mg/kg the IFP low dose group tumor killing effect of dose groups and 50mg/kg be respectively 25.00% and 15.54%; All demonstrate certain tumor killing effect, IFP is described simultaneously to the increase along with dosage of the inhibitory action of HepG2 transplanted tumor, its tumour inhibiting rate increases gradually.
Embodiment 2: observe the inhibitory action of IFP to mice S180 sarcoma:
Mice S180 sarcoma cell: purchase in Nat'l Pharmaceutical & Biological Products Control Institute.
Cat Isaria crude polysaccharides, it is made by following steps: get the mycelium powder that 300g embodiment 1 makes, add the 900ml distilled water, boiling water bath extracts 110min, the centrifugal 6min of 3000r/min; In deposition, continue to add the 900ml distilled water, boiling water bath extracts 20min, the centrifugal 4min of 3000r/min; In deposition, continue to add the 900ml distilled water, boiling water bath extracts 20min, the centrifugal 6min of 3000r/min; Merge the supernatant of three extractions, water-bath is concentrated into 1000mL, gets mixed liquor; 95% ethanol that adds 3 times of mixeding liquid volumes, abundant mixing, deposition is spent the night; Get deposition, in 70 ℃ of dry for standby.
Other experiment material, experimental technique are with mice H22 HCC among the embodiment 1, and its experimental result is as shown in table 3 below:
Table 3 IFP is to the inhibitory action of mice transplantability meat cancer S180
Annotate: * and negative control group are relatively
P<0.05
Experimental result shows that compare with negative control group, mitomycin and IFP have the good restraining effect to S180 transplanted tumor, and wherein the tumor killing effect of mitomycin is best, has reached 64.47%, compares with negative control group to have significant difference; IFP also has the good restraining effect to S180 transplanted tumor; Compare with negative control group and to have significant difference; The IFP high dose group inhibitory rate of 200mg/kg to 44.32% and among the IFP of 100mg/kg the IFP low dose group tumor killing effect of dose groups and 50mg/kg be respectively 39.56% and 33.33%; All demonstrate certain tumor killing effect, IFP is described simultaneously to the increase along with dosage of the inhibitory action of S180 transplanted tumor, its tumour inhibiting rate increases gradually.
Embodiment 3: observe the inhibitory action of IFP to people Hela cervical cancer cell:
People Hela cervical cancer cell: purchase in China Concord Medical Science University.
Cat Isaria crude polysaccharides, it is made by following steps: get the mycelium powder that 300g embodiment 1 makes, add the 1500ml distilled water, boiling water bath extracts 70min, the centrifugal 4min of 3000r/min; In deposition, continue to add the 1500ml distilled water, boiling water bath extracts 40min, the centrifugal 6min of 3000r/min; In deposition, continue to add the 1500ml distilled water, boiling water bath extracts 40min, the centrifugal 4min of 3000r/min; Merge the supernatant of three extractions, water-bath is concentrated into 800mL, gets mixed liquor; 95% ethanol that adds 4 times of mixeding liquid volumes, abundant mixing, deposition is spent the night; Get deposition, in 70 ℃ of dry for standby.
Other experiment material, experimental technique are with the people HepG2 HCC among the embodiment 1, and its result is as shown in table 4 below:
Table 4 IFP is to the inhibitory action of nude mice transplantability cervical cancer Hela
Annotate: * and negative control group are relatively
P<0.05
Experimental result shows, compares with negative control group, and mitomycin and IFP have the good restraining effect to Hela transplanted tumor, and wherein the tumor killing effect of mitomycin is best, and inhibitory rate is to 59.21%, compares with negative control group to have significant difference; IFP also has the good restraining effect to Hela transplanted tumor; Compare with negative control group and to have significant difference; The IFP high dose group inhibitory rate of 200mg/kg to 38.16% and among the IFP of 100mg/kg the IFP low dose group tumor killing effect of dose groups and 50mg/kg be respectively 25.00% and 19.08%; All demonstrate certain tumor killing effect, IFP is described simultaneously to the increase along with dosage of the inhibitory action of Hela transplanted tumor, its tumour inhibiting rate increases gradually.
Embodiment 4: observe the life prolongation effect of IFP to mice EAC ascites cells:
Experiment material: mice EAC ascites cells, purchase in Nat'l Pharmaceutical & Biological Products Control Institute.
Cat Isaria crude polysaccharides, it is made by following steps: get the mycelium powder that 300g embodiment 1 makes, add the 1100ml distilled water, boiling water bath extracts 100min, the centrifugal 5min of 3000r/min; In deposition, continue to add the 1100ml distilled water, boiling water bath extracts 26min, the centrifugal 6min of 3000r/min; In deposition, continue to add the 1100ml distilled water, boiling water bath extracts 35min, the centrifugal 4min of 3000r/min; Merge the supernatant of three extractions, water-bath is concentrated into 950mL, gets mixed liquor; 95% ethanol that adds 3.5 times of mixeding liquid volumes, abundant mixing, deposition is spent the night; Get deposition, in 70 ℃ of dry for standby.
Other experiment material is with the mice H22 HCC among the embodiment 1.
Experimental technique: aseptic condition extracts well-grown kunming mice EAC ascites down, and using normal saline to regulate concentration of cell suspension is 5 * 10
6/ mL, the abdominal cavity is inoculated in the abdominal part of kunming mice, every kunming mice inoculation 0.2mL tumor liquid.Group technology and gastric infusion dosage are observed 30d with the mice H22 HCC among the embodiment 1, the record survival time of animals, and life span surpasses 30d person by 30d, and its result is as shown in table 5 below:
Table 5 IFP is to the life prolongation effect of ehrlich ascites carcinoma EAC mice
| Group | Number of animals (end/beginning) | Mean survival time (d) | Increase in life span (%) |
| Negative control group | 0/9 | 15.20±8.35 | - |
| The mitomycin group | 1/9 | 19.60±10.36 | 28.95 |
| The Fructus Bruceae line of oils | 0/9 | 22.50±8.11 | 48.03 |
| The IFP high dose group | 2/9 | 27.24±8.24* | 79.21 |
| Dose groups among the IFP | 1/9 | 24.33±11.45* | 60.07 |
| The IFP low dose group | 1/9 | 20.33±9.12 | 33.75 |
Annotate: * and negative control group are relatively
P<0.05
Experimental result shows that compare with negative control group, Fructus Bruceae line of oils and IFP have good life prolongation effect to ehrlich ascites carcinoma EAC mice, and wherein the increase in life span of Oleum Fructus Bruceae has reached 48.03%, compares with negative control group to have significant difference; IFP also has good life prolongation effect to ehrlich ascites carcinoma EAC mice; Compare with negative control group and to have significant difference; The IFP high dose group of 200mg/kg reached 79.21% and among the IFP of 100mg/kg the IFP low dose group tumor killing effect of dose groups and 50mg/kg be respectively 60.07% and 33.75%; All demonstrate certain life and prolong effect, explain that simultaneously IFP increases along with the increase of dosage the increase in life span of ehrlich ascites carcinoma EAC mice gradually.
Claims (1)
1. cat Isaria crude polysaccharides is as the application of preparation antitumor drug; Wherein: said cat Isaria crude polysaccharides is made by following steps: get cat Isaria mycelium powder; The distilled water that adds 3-5 times of mycelium powder weight, boiling water bath extracts 70-110min, centrifugal 4-6min under the 3000r/min rotating speed; In deposition, continue to add the distilled water of 3-5 times of mycelium powder weight, boiling water bath extracts 20-40min, centrifugal 4-6min under the 3000r/min rotating speed; In deposition, continue to add the distilled water of people 3-5 times of mycelium powder weight, boiling water bath extracts 20-40min, the centrifugal 4-6min of 3000r/min; Merge the supernatant of three extractions, water-bath is concentrated into 800-1000mL, gets mixed liquor; 95% ethanol that adds 3-4 times of mixeding liquid volume, abundant mixing, deposition is spent the night; Get deposition, in 70 ℃ dry cat Isaria crude polysaccharides.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110664849A (en) * | 2019-11-05 | 2020-01-10 | 山西省肿瘤研究所 | Application of cat isaria mycelium |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1436841A (en) * | 2002-02-08 | 2003-08-20 | 河北神兴沙棘研究院 | Fine rod bundle spore SX-1 separated and cultured from cordyceps and its saparation, culture process and use |
| CN102228471A (en) * | 2011-06-27 | 2011-11-02 | 山西大学 | Use of cat isaria mycelium |
| CN102585024A (en) * | 2012-01-15 | 2012-07-18 | 山西大学 | Isaria feline crude polysaccharide, and preparation method and application thereof |
-
2012
- 2012-08-18 CN CN2012102941015A patent/CN102813669A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1436841A (en) * | 2002-02-08 | 2003-08-20 | 河北神兴沙棘研究院 | Fine rod bundle spore SX-1 separated and cultured from cordyceps and its saparation, culture process and use |
| CN102228471A (en) * | 2011-06-27 | 2011-11-02 | 山西大学 | Use of cat isaria mycelium |
| CN102585024A (en) * | 2012-01-15 | 2012-07-18 | 山西大学 | Isaria feline crude polysaccharide, and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 任宝生等: "细棒束孢培养物对小鼠移植性肿瘤抑制作用的实验研究", 《世界中西医结合杂志》, vol. 2, no. 12, 31 December 2007 (2007-12-31) * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110664849A (en) * | 2019-11-05 | 2020-01-10 | 山西省肿瘤研究所 | Application of cat isaria mycelium |
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