CN102805282A - Method for preparing and producing panax quinguefolium polysaccharides peptide - Google Patents
Method for preparing and producing panax quinguefolium polysaccharides peptide Download PDFInfo
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- CN102805282A CN102805282A CN2011101570901A CN201110157090A CN102805282A CN 102805282 A CN102805282 A CN 102805282A CN 2011101570901 A CN2011101570901 A CN 2011101570901A CN 201110157090 A CN201110157090 A CN 201110157090A CN 102805282 A CN102805282 A CN 102805282A
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- american ginseng
- alcohol
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- water
- polysaccharide peptide
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- 150000004676 glycans Chemical class 0.000 title description 6
- 229920001282 polysaccharide Polymers 0.000 title description 6
- 239000005017 polysaccharide Substances 0.000 title description 6
- 108090000765 processed proteins & peptides Proteins 0.000 title description 4
- 238000000034 method Methods 0.000 title description 3
- 235000002791 Panax Nutrition 0.000 title description 2
- 241000208343 Panax Species 0.000 title description 2
- 240000005373 Panax quinquefolius Species 0.000 claims abstract description 33
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 33
- 229920001231 Polysaccharide peptide Polymers 0.000 claims abstract description 22
- 108010022457 polysaccharide peptide Proteins 0.000 claims abstract description 22
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000000284 extract Substances 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 230000036039 immunity Effects 0.000 claims abstract description 8
- 239000002244 precipitate Substances 0.000 claims abstract description 8
- 239000008280 blood Substances 0.000 claims abstract description 7
- 210000004369 blood Anatomy 0.000 claims abstract description 7
- 239000012141 concentrate Substances 0.000 claims abstract description 6
- 239000002775 capsule Substances 0.000 claims abstract description 5
- 229930182490 saponin Natural products 0.000 claims abstract description 5
- 150000007949 saponins Chemical class 0.000 claims abstract description 5
- 102000004190 Enzymes Human genes 0.000 claims abstract description 4
- 108090000790 Enzymes Proteins 0.000 claims abstract description 4
- 235000019658 bitter taste Nutrition 0.000 claims abstract description 4
- 239000000706 filtrate Substances 0.000 claims abstract description 4
- 239000000796 flavoring agent Substances 0.000 claims abstract description 4
- 235000019634 flavors Nutrition 0.000 claims abstract description 4
- 239000002994 raw material Substances 0.000 claims abstract description 4
- 239000007921 spray Substances 0.000 claims abstract description 4
- 108091005804 Peptidases Proteins 0.000 claims abstract description 3
- 239000004365 Protease Substances 0.000 claims abstract description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims abstract description 3
- 238000004519 manufacturing process Methods 0.000 claims abstract description 3
- 239000001397 quillaja saponaria molina bark Substances 0.000 claims abstract description 3
- 239000006228 supernatant Substances 0.000 claims abstract description 3
- 239000007787 solid Substances 0.000 claims abstract 2
- 239000000447 pesticide residue Substances 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 235000017709 saponins Nutrition 0.000 description 3
- 230000036772 blood pressure Effects 0.000 description 2
- 238000001694 spray drying Methods 0.000 description 2
- 235000019640 taste Nutrition 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 229930182494 ginsenoside Natural products 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000751 protein extraction Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 230000009967 tasteless effect Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
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- Non-Alcoholic Beverages (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
本发明公开了一种西洋参多糖肽制备生产方法,用西洋参茎、叶、须根为原料,以水提取,料液比1∶10,在60℃,提取时间2h,两次,过滤合并滤液,真空浓缩滤浓成10∶1,75%酒精沉淀,离心取上清液回收酒精并提取皂苷,再用95%食用酒精洗脱沉淀物,回收酒精。对沉淀物按1∶10加水溶解,加入1%风味蛋白酶,在55℃,PH5.6,水解4h,灭酶,过滤真空浓缩,喷雾干燥,制成灰白色的西洋参多糖肽,解决了西洋参提取物味苦的问题。西洋参多糖肽可直接冲服,也可制成保健饮料和胶囊产品,西洋参多糖肽具有提高人体免疫力,降低血糖的作用。The invention discloses a production method of American ginseng polysaccharide peptide, which uses American ginseng stems, leaves and fibrous roots as raw materials, extracts with water, the ratio of solid to liquid is 1:10, extracts twice at 60°C for 2 hours, filters the combined filtrate, and vacuum Concentrate and filter to a ratio of 10:1, precipitate with 75% alcohol, centrifuge the supernatant to recover alcohol and extract saponin, then use 95% edible alcohol to elute the precipitate and recover alcohol. Dissolve the precipitate with water at a ratio of 1:10, add 1% flavor protease, hydrolyze for 4 hours at 55°C, pH 5.6, inactivate the enzyme, filter, concentrate in vacuum, and spray dry to make off-white polysaccharide peptide of American ginseng, which solves the problem of American ginseng extract Bitterness problem. American ginseng polysaccharide peptide can be taken directly or made into health drinks and capsules. American ginseng polysaccharide peptide can improve human immunity and lower blood sugar.
Description
技术领域 technical field
本发明涉及西洋参多糖肽的制备生产方法,利用西洋参茎、叶、根,通过水提醇沉、真空浓缩、酶解、喷雾干燥而制备生产西洋参多糖肽。The invention relates to a preparation and production method of American ginseng polysaccharide peptide. The American ginseng polysaccharide peptide is prepared and produced by using American ginseng stems, leaves and roots through water extraction and alcohol precipitation, vacuum concentration, enzymolysis and spray drying.
背景技术 Background technique
西洋参(Panax quinquefoium L.)系五加科人参属多年生药用植物,原产于北美洲,后传入中国,是一种名贵滋补药品。西洋参味苦微甘,入心、肺、胃三经,有大补元气、益血生津、降压解热等作用。西洋参茎、叶及根含人参皂苷,还有蛋白质、多肽、氨基酸、多糖、脂肪酸、微量元素、挥发油等。西洋参皂苷作用在于提高免疫力和抗疲劳,但味苦,而西洋参多糖及多肽无味,不但能够高免疫力外,还具有降低血压作用。利用废弃的西洋参茎、叶和西洋参须根提取并分离皂苷和多糖肽,再酶解成多糖肽,使多糖肽具有提高免疫力降低血糖的作用,解决一西洋参深加工中味苦的难题,并能降低西洋参茎和叶中农药残留的影响。American ginseng (Panax quinquefoium L.) is a perennial medicinal plant of the Araliaceae ginseng genus. It is native to North America and later introduced to China. It is a precious nourishing medicine. American ginseng tastes bitter and slightly sweet, enters the heart, lung, and stomach meridians, and has the functions of invigorating vitality, nourishing blood and promoting body fluid, reducing blood pressure and relieving heat. American ginseng stems, leaves and roots contain ginsenosides, as well as proteins, polypeptides, amino acids, polysaccharides, fatty acids, trace elements, and volatile oils. The role of American ginseng saponins is to improve immunity and anti-fatigue, but it tastes bitter, while American ginseng polysaccharides and polypeptides are tasteless, not only can improve immunity, but also have the effect of lowering blood pressure. Using discarded American ginseng stems, leaves and American ginseng fibrous roots to extract and separate saponins and polysaccharide peptides, and then enzymatically hydrolyze them into polysaccharide peptides, so that polysaccharide peptides can improve immunity and lower blood sugar, solve the problem of bitter taste in the deep processing of American ginseng, and reduce the risk of American ginseng Effects of pesticide residues in stems and leaves.
发明内容 Contents of the invention
本发明利用西洋参茎、叶、须根为原料,以水提取,料液比1∶10,在60℃,,提取时间2h,提取两次,过滤合并滤液,真空浓缩滤浓成10∶1,食用酒精75%沉淀,离心取上清液回收酒精并提取皂苷,再用95%食用酒精洗脱沉淀物,回收酒精。对沉淀物按1∶10加水溶解,加入1%风味蛋白酶,在55℃,PH5.6,水解4h,灭酶,过滤真空浓缩,喷雾干燥,制成灰白色的多糖肽,解决了西洋参提取物味苦的难题,并能降低西洋参茎和叶中农药残留。西洋参多糖肽作制成保健饮料和胶囊,具有提高人体免疫力,减低血糖的作用。The present invention uses American ginseng stems, leaves and fibrous roots as raw materials, extracts with water, the ratio of material to liquid is 1:10, at 60°C, the extraction time is 2h, extracts twice, filters and merges the filtrate, vacuum concentrates and filters to a concentration of 10:1, and eats 75% alcohol was precipitated, and the supernatant was centrifuged to recover alcohol and extract saponin, and then 95% edible alcohol was used to elute the precipitate to recover alcohol. Dissolve the precipitate with water at a ratio of 1:10, add 1% flavor protease, hydrolyze at 55°C, pH 5.6, for 4 hours, inactivate the enzyme, filter, concentrate in vacuo, and spray dry to make off-white polysaccharide peptide, which solves the smell of American ginseng extract Bitter problem, and can reduce pesticide residues in American ginseng stems and leaves. American ginseng polysaccharide peptides are made into health drinks and capsules, which can improve human immunity and reduce blood sugar.
因此,利用本工艺生产西洋参多糖肽,色泽灰白、无苦味、速溶性好、能够加工成保健饮料或胶囊,也可直接水冲饮用,属国内外首创。西洋参多糖肽对于人体具有提高免疫力,降低血糖的保健作用。Therefore, the use of this process to produce American ginseng polysaccharide peptides has off-white color, no bitter taste, good instant solubility, can be processed into health drinks or capsules, and can also be directly brewed with water, which is the first at home and abroad. American ginseng polysaccharide peptide has the health care effect of improving immunity and lowering blood sugar for human body.
下面结合实施例对本发明作进一步详细的说明。Below in conjunction with embodiment the present invention is described in further detail.
实施例子Implementation example
①原料:西洋参茎、叶、西洋参加工的剩余的须根。① Raw materials: American ginseng stems, leaves, remaining fibrous roots of American ginseng workers.
②多糖蛋白提取:西洋参茎、叶,加入10倍的水,在60℃,提取2H,提取2次,过滤合并滤液,真空浓缩成10∶1,乙醇浓度75%沉淀,再用95%乙醇洗脱,得到多糖蛋白。② Polysaccharide protein extraction: American ginseng stems and leaves, add 10 times of water, extract at 60°C for 2 hours, extract twice, filter and combine the filtrate, concentrate in vacuum to 10:1, precipitate with ethanol concentration of 75%, and then wash with 95% ethanol Take off to obtain polysaccharide protein.
③多肽水解:多糖蛋白加入10倍的水,在55℃,PH5.6,酶解4H,灭酶。③ Polypeptide hydrolysis: add 10 times of water to polysaccharide protein, enzymatically hydrolyze for 4 hours at 55°C, pH 5.6, and inactivate the enzyme.
④真空浓缩:对水解液经过真空浓缩,质量数达到10%以上。④Vacuum concentration: the hydrolyzate is concentrated in vacuum, and the mass number reaches more than 10%.
⑤喷雾干燥:喷雾干燥多糖肽,进行粉碎,过筛成100目。⑤ Spray drying: Spray dry the polysaccharide peptide, pulverize it, and sieve it into 100 mesh.
⑥包装:将多糖肽用塑料进行包装。⑥Packaging: Pack the polysaccharide peptide with plastic.
⑦使用:用开水直接冲服多糖肽,或将多糖肽配制成饮料和制胶囊食用,具有提高人体免疫力,降低血糖作用。⑦Usage: Take the polysaccharide peptide directly with boiling water, or make the polysaccharide peptide into a beverage and make capsules for consumption, which can improve human immunity and lower blood sugar.
Claims (4)
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106397625A (en) * | 2016-10-28 | 2017-02-15 | 宁波中药制药股份有限公司 | Method for extracting panax quinquefolius polysaccharides from panax quinquefolius fibrous roots |
CN113577239A (en) * | 2021-07-30 | 2021-11-02 | 山东第一医科大学(山东省医学科学院) | American ginseng glycopeptide and preparation method and application thereof |
CN114032273A (en) * | 2021-11-17 | 2022-02-11 | 山东省科学院生物研究所 | Multifunctional American ginseng hydrolyzed peptide and preparation method and application thereof |
CN114805521A (en) * | 2022-06-07 | 2022-07-29 | 清枫链食苏打饮品(吉林)有限公司 | American ginseng polypeptide and composition thereof and application of American ginseng polypeptide and composition thereof in lowering blood pressure and improving immunity |
CN115089697A (en) * | 2022-08-25 | 2022-09-23 | 广东泰升药业有限公司 | American ginseng glycopeptide and preparation method and application thereof |
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JPH0236124A (en) * | 1988-07-26 | 1990-02-06 | Osaka Prefecture | Antiviral agent |
JPH0245499A (en) * | 1988-08-06 | 1990-02-15 | Wakunaga Pharmaceut Co Ltd | Glycoprotein of panax ginseng and use thereof |
CN1054898A (en) * | 1990-09-07 | 1991-10-02 | 徐世忱 | Carry the glycoside dreg liquid compound ginseng element of purifying from Stem and leaf of Radix Ginseng |
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2011
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Patent Citations (5)
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JPH0236124A (en) * | 1988-07-26 | 1990-02-06 | Osaka Prefecture | Antiviral agent |
JPH0245499A (en) * | 1988-08-06 | 1990-02-15 | Wakunaga Pharmaceut Co Ltd | Glycoprotein of panax ginseng and use thereof |
CN1054898A (en) * | 1990-09-07 | 1991-10-02 | 徐世忱 | Carry the glycoside dreg liquid compound ginseng element of purifying from Stem and leaf of Radix Ginseng |
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Title |
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106397625A (en) * | 2016-10-28 | 2017-02-15 | 宁波中药制药股份有限公司 | Method for extracting panax quinquefolius polysaccharides from panax quinquefolius fibrous roots |
CN106397625B (en) * | 2016-10-28 | 2019-09-20 | 宁波中药制药股份有限公司 | A method of extracting Radix Panacis Quinquefolii polysaccharide from American Ginseng fibrous root |
CN113577239A (en) * | 2021-07-30 | 2021-11-02 | 山东第一医科大学(山东省医学科学院) | American ginseng glycopeptide and preparation method and application thereof |
CN114032273A (en) * | 2021-11-17 | 2022-02-11 | 山东省科学院生物研究所 | Multifunctional American ginseng hydrolyzed peptide and preparation method and application thereof |
CN114032273B (en) * | 2021-11-17 | 2024-02-02 | 山东省科学院生物研究所 | A multifunctional American ginseng hydrolyzed peptide and its preparation method and application |
CN114805521A (en) * | 2022-06-07 | 2022-07-29 | 清枫链食苏打饮品(吉林)有限公司 | American ginseng polypeptide and composition thereof and application of American ginseng polypeptide and composition thereof in lowering blood pressure and improving immunity |
CN115089697A (en) * | 2022-08-25 | 2022-09-23 | 广东泰升药业有限公司 | American ginseng glycopeptide and preparation method and application thereof |
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