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CN102805170B - Black tea fungus beverage as well as preparation technology and application thereof - Google Patents

Black tea fungus beverage as well as preparation technology and application thereof Download PDF

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Publication number
CN102805170B
CN102805170B CN2012103118862A CN201210311886A CN102805170B CN 102805170 B CN102805170 B CN 102805170B CN 2012103118862 A CN2012103118862 A CN 2012103118862A CN 201210311886 A CN201210311886 A CN 201210311886A CN 102805170 B CN102805170 B CN 102805170B
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black
tea
parts
water
bacterium liquid
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CN102805170A (en
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邝志龙
邝志强
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DONGGUAN JIANMEIZI BEVERAGE FOOD Co Ltd
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DONGGUAN JIANMEIZI BEVERAGE FOOD Co Ltd
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Abstract

The invention belongs to the technical field of the food beverage, which particular relates to a black tea fungus beverage as well as a preparation technology and application thereof. The tea fungus beverage consists of the following raw materials in parts by weight: 1000 parts of water, 100-300 parts of black tea fungus bacteria liquid, 1-10 parts of red date, 1-5 parts of longan, 1-5 parts of hawthorn, 0-5 parts of dried lichee, 2-10 parts of black tea, 0-5 parts of sea-buckthorn and 50-200 parts of sweetening agent. The black tea fungus beverage has the health-care functions such as appetizing, invigorating stomach, aiding digestion and increasing appetite, has a good promoting function on intestinal tract diseases, has the advantages of varied tastes and abundant nutrition ingredients, is suitable for both young and old and can be directly drunk by people. According to the preparation technology of the product, the black tea fungus bacteria liquid is used for preparing, and various raw materials are mixed and heated after being pre-processed and are sterilized and disinfected after being filled. The preparation technology is simple to operate, is safe and clean to operate, andis suitable for industrial production, and the production cost is lowered. The product has the functions of eliminating fat, resisting oxidation, enhancing resistance, maintaining beauty and keep young and the like.

Description

A kind of black-tea fungus drink and preparation technology thereof and application thereof
Technical field
The invention belongs to technical field of beverage, particularly a kind of black-tea fungus drink and preparation technology thereof and application thereof.
Background technology
Fermented tea has another name called " Hypon ", " stomach treasured ", and it is to be raw material with sugar and tea, and fermenting through lactic acid bacteria, saccharomycete and acetic acid bacteria forms.Contain the extract of some tealeaves, microorganism and the metabolite thereof of living in the fermented tea bacterium liquid, these materials mainly comprise gluconic acid, acetic acid, glucose, fructose, protein, amino acid, vitamin, trace element, Tea Polyphenols, caffeine, ethanol and carbon dioxide etc.The acidity of itself has suppressed the growth of harmful bacteria again, and health is highly profitable, and especially atrophic gastritis, gastric ulcer difficult disease is had good therapeutic action.
Utilize these functions of fermented tea, people begin fermented tea is added in the middle of the various food, Fructus Jujubae-black tea fungus beverage and brewing method as disclosed patent of invention: CN1593252A, emphasis has added red date as the ferment base, ferment with fermented tea, draw the black-tea fungus drink with red date taste and blood nourishing function.Patent of invention 200410050571.2, a kind of production method of black-tea fungus drink is added highly enriched fruit juice and is carried out the fermented tea fermentation in the culture medium of fermented tea, make the black-tea fungus drink with various fruit tastes.
All there is same problem in above technology, be exactly additive be before fermentation, to add in the culture medium of fermented tea, the black-tea fungus drink that obtains all contains the fermented tea of high concentration, and fermented tea itself is to have bigger acidity, generally can not directly drink, need the later stage oneself to add water or sugar and wait other flavor enhancements to modulate just to drink.The beverage of oneself making, proportioning are difficult to grasp, and the mouthfeel quality instability can not satisfy most of consumer demand.Another deficiency is, the black-tea fungus drink of making still is the live body bacterium, can continue fermentation after can, and the holding time is shorter.Oxygen in tank body is fermented have been utilized, and will produce other harmful substances such as carbon dioxide, and can produce a large amount of sediments, not only influences the outward appearance of black-tea fungus drink, also can influence the quality of fermented tea.Arrive to a certain degree when fermenting, the gas in the tank body increases, and the danger of bottle explosion then can take place.
Aspect preparation technology, because additive is to add to before fermentation in the fermentation base, than higher, sweat can be subjected to environment to dimension to the purity requirement of additive, temperature, the influence of time etc. aspect, condition is difficult to control, preparation requires than higher, complicated operation, quality instability.
Summary of the invention
The objective of the invention is to provides a kind of black-tea fungus drink at the deficiencies in the prior art, it has the popular taste of being fit to, drink conveniently, the time of guaranteeing the quality is long and nutritious, have appetizing, stomach invigorating, aid digestion, the health care that increases appetite etc., strengthen resistance of human body, stomach is had facilitation preferably.
Another object of the present invention is to provide a kind of preparation technology of black-tea fungus drink, preparation technology is simple to operate for this skill, and production cost is low, and quality is fit to suitability for industrialized production easily control.
Another object of the present invention is to provide a kind of application of black-tea fungus drink, in strengthening human body tolerance, resistance and anti peroxidation of lipid, health-care effect is preferably arranged.
The object of the present invention is achieved like this.
A kind of black-tea fungus drink, formed by the raw material of following weight portion:
1000 parts in water
Fermented tea bacterium liquid 100-300 part
Red date 1-10 part
Longan 1-5 part
Hawthorn 1-5 part
Lichee is done 0-5 part
Black tea 2-10 part
Sea-buckthorn 0-5 part
Sweetener 50-200 part,
Wherein, described fermented tea bacterium liquid is the mixed liquor of fermented tea bacterial classification, mother liquor and black tea water stoste, and wherein, described sweetener comprises white sugar, rock sugar, fructose, honey, glucose, any of several broadleaf plants sugar.
Preferably, formed by the raw material of following weight portion:
1000 parts in water
Fermented tea bacterium liquid 140-250 part
Red date 3-6 part
Longan 1-3 part
Hawthorn 1-3 part
Lichee is done 1-3 part
Black tea 4-6 part
Sea-buckthorn 1-3 part
Sweetener 100-150 part.
Preferred, formed by the raw material of following weight portion:
1000 parts in water
200 parts of fermented tea bacterium liquid
4 parts of red dates
2 parts of longan
2 parts of hawthorn
Lichee is done 2 parts
5 parts of black teas
2 parts of sea-buckthorns
80 parts of sweeteners.
Fermented tea is to be formed by black tea, white sugar and water brew.The fermented tea bacterial classification that relates among the present invention and mother liquor all are can directly buy gained on the market, are not limited to the fermented tea that is prepared from by following basic cultural method:
(1) cultivation of mother liquor: in boiling water, add earlier black tea, white sugar a little, the black tea syrup that furnishing is rare, slightly boil sterilization again after, namely contain in the sterile glass, when being cooled to 35 ℃, add a black tea bacterial classification big as finger (can buy gained on the market) again, seal rim of a cup with clean gauze, three, after four days, cup bears green cilium in an end, and liquid level grows mycoderm like the jellyfish, in a few days, the souring of tea liquid, flavor has just been made mother liquor as red bayberry soup.
(2) culture of strains: culture of strains is to carry out on cultivation mother liquor basis.The cenobium that generates in the mother liquor has multilayer, and lower floor is tenderer and bright, should make bacterial classification.
The material rate of cultivating fermented tea is black tea: sugar: water=2:10:100.
Fermented tea bacterium liquid among the present invention is to be that the ratio of 1-5:1:10-15 mixes by the mother liquor, black tea water stoste of fermented tea bacterial classification, the corresponding fermented tea bacterial classification of preparation according to weight portion, and concrete proportioning is specifically allocated according to the difference of taste.
Black tea water stoste among the present invention is that the weight portion ratio 1-3:10:100 by tealeaves, sugar, water is prepared from.
In the raw material of the present invention, red date distinguish the flavor of sweet warm in nature, return the taste warp, the function of tonifying middle-Jiao and Qi, nourishing blood and tranquilization, the mitigation property of medicine is arranged; Modern pharmacology finds that then red date contains abundant nutrition such as protein, fat, carbohydrate, organic acid, vitamin A, vitamin C, micro-calcium several amino acids; Be rich in calcium and iron in the red date, they play an important role to preventing and treating the osteoporosis postpartum anemia, middle-aged and old climacteric are through regular meeting's osteoporosis, anaemia takes place in growing teenager and the women on peak easily, date has very good dietary function to them, and its effect normally medicine can not compared; The rutin that red date is contained is a kind of angiomalasia that makes, thereby the material that blood pressure is reduced has prophylactic-therapeutic effect to high blood pressure.
Longan also claims longan (longan, first meat), can be used as medicine, multiple efficacies such as establishing-Yang benefit gas, tonifying heart and spleen, nourishing blood and tranquilization, moistening skin beautifying are arranged, can treat anaemia, palpitaition, insomnia, forgetful, neurasthenia and after being ill, postpartum is in poor health etc., and the disease arillus longan has tonifying heart and spleen, the effect of nourishing blood and tranquilization.
Hawthorn does medicinal with fruit, slightly warm in nature, and flavor acid is sweet, goes into spleen, stomach, Liver Channel, food digesting stomach fortifying is arranged, activate blood circulation and disperse blood clots, restrain the only function of dysentery.1. can prevent and treat angiocardiopathy, have hemangiectasis, increase CF, improve the heart vigor, the stimulating central nervous system system, bring high blood pressure down and cholesterol, softening blood vessel and diuresis and sedation; 2. crataegolic acid also has cardiotonic, and is also helpful to senile heart disease; 3. hawthorn has the effect that activates blood circulation and disperses blood clots, and helps to remove local extravasated blood state, and traumatic injury is had auxiliary curative effect; 4. the contained flavonoids of hawthorn and materials such as vitamin C, the carrotene generation that can block and reduce free radical can strengthen the immunity of body, and anti-aging, anticancer effect is arranged; 5. having in the hawthorn relievings asthma reduces phlegm, suppresses bacterium, the treatment abdominal pain diarrhea composition. 
Lichee is done, and gets fresh litchi and dries or dry, and nature and flavor and function that lichee is done: (1) nature and flavor: it is sweet, sour, warm in nature to distinguish the flavor of.(2) function: beneficial heart kidney, nourishing the liver blood; Can control woman's anaemia, infantile enuresis, deficiency of vital energy chilly, splenasthenic diarrhea etc.
Sea buckthorn fruit is nutritious, contains each seed amino acid of multivitamin, aliphatic acid, trace element, inferior oil element, Hippophate flavone, superoxides isoreactivity material and needed by human body according to surveying and determination in its fruit.Wherein Vitamin C content is high, and in per 100 gram fruit juice, Vitamin C content can reach 825-1100 milligrams, is 2-3 times of Kiwi berry, have the king's of vitamin C laudatory title.Contain sugar 7.5%-10%, contain acid 3%-5%; Fructus hippophae and oil have very high medical value, can reduce cholesterol, and the allevating angina pectoris outbreak prevents and treats the effect of coronary atherosclerotic heart disease in addition; Have and eliminate the phlegm, cough-relieving, relieving asthma and treat the effect of chronic bronchitis; Can treat gastric duodenal ulcer and indigestion etc., evident in efficacy to illnesss such as chronic superficial gastritis, atrophic gastritis, colitis.
White sugar, rock sugar, fructose, honey, glucose, any of several broadleaf plants sugar etc. are the edible natural sweeteners of using always, and the rock sugar flavor is sweet, property flat, goes into lung, the spleen channel; The effect that tonifying middle-Jiao and Qi and stomach moistening lung are arranged; The rock sugar nourishing Yin and promoting production of body fluid moistens the lung and relieve the cough, to xeropulmonary cough, dry cough without phlegm, cough up sputum streaked with blood good auxiliary curing effect is arranged.Natural sweetener edible safety, the nutrient content height is suitable for mass consumption person.
Can also comprise xylitol and synanthrin, it has certain sugariness, and heat is few, generally is not absorbed by the body, thereby suitable overweight people and glycosuria patient's demand.
A kind of preparation technology of black-tea fungus drink, it has following processing step:
(a) preparation of fermented tea bacterium liquid: the fermented tea bacterial classification that the market is buied is added in mother liquor and the black tea water stoste, under 25 ℃~35 ℃ temperature, fermented 1~3 month in the clean environment, when the test pH value 3.0 below fermented tea bacterium liquid, filtration, standby;
(b) preliminary treatment of various raw materials: black tea is made black tea water through lixiviate, and heating refilters then, and is standby;
Red date, lichee do, one or several combination in longan, hawthorn and the sea-buckthorn rinses well, and is standby;
(c) mix: in joining cylinder, add pure water, add that pretreated red date, lichee among the fermented tea bacterium liquid for preparing among the step a and the step b are done, one or several the combination of longan, hawthorn, sea-buckthorn, be heated to 100 ℃, heated 5~20 minutes, the black tea water of handling among the blend step b again, add sweetener and mix, filter the back can;
(d) sterilization treatment: the black-tea fungus drink after the can was through 80 ℃~100 ℃ pasteurizes 30~50 minutes.
Wherein, the fermented tea bacterium liquid fermentation time in the described step (a) is 2 months.
Wherein, be 10~15 minutes the heat time heating time in the described step (c).
Wherein, the sterilising temp in the described step (d) is 85 ℃~95 ℃, and sterilizing time is 35~45 minutes.
Wherein: the red date among the described step b, lichee are done, one or several combination of longan, hawthorn and sea-buckthorn system is rinsed well, after waiting to do, are prepared into powdery, and be standby.
Beneficial effect of the present invention: a kind of black-tea fungus drink of the present invention, by the raw material of following weight portion: 1000 parts in water, fermented tea bacterium liquid 100-300 part, red date 1-10 part, longan 1-5 part, hawthorn 1-5 part, lichee is done 0-5 part, black tea 2-10 part, sea-buckthorn 0-5 part, sweetener 50-200 part.A kind of black-tea fungus drink of the present invention, use traditional theory of traditional Chinese medical science and modern science compatibility, mix with other materials with the fermented tea that ferments, enterogastritis is had facilitation preferably, taste is changeable, nutritional labeling is abundant, health care is comprehensive, and is suitable for all ages, and people can directly drink, the fat that can disappear is anti-oxidant, builds up resistance, effect such as beautifying face and moistering lotion.
A kind of preparation technology of black-tea fungus drink, it has following processing step:
(a) preparation of fermented tea bacterium liquid: the fermented tea bacterial classification that the market is buied is added in mother liquor and the black tea water stoste, under 25 ℃~35 ℃ temperature, fermented 1~3 month in the clean environment, when the test pH value 3.5 below fermented tea bacterium liquid, filtration, standby;
(b) preliminary treatment of various raw materials: black tea is made black tea water through lixiviate, and heating refilters then, and is standby;
Red date, lichee do, one or several combination in longan, hawthorn and the sea-buckthorn rinses well, and is standby;
(c) mix: in joining cylinder, add pure water, add that pretreated red date, lichee among the fermented tea bacterium liquid for preparing among the step a and the step b are done, one or several the combination of longan, hawthorn, sea-buckthorn, be heated to 100 ℃, heated 5~20 minutes, the black tea water of handling among the blend step b again, add sweetener and mix, filter the back can;
(d) sterilization treatment: the black-tea fungus drink after the can was through 80 ℃~100 ℃ pasteurizes 30~50 minutes.
The preparation technology of a kind of black-tea fungus drink of the present invention, simple to operate, reduced production cost, the handling safety health is fit to suitability for industrialized production.
The application of a kind of black-tea fungus drink that this law is bright has health-care effect preferably in strengthening human body tolerance, resistance and anti peroxidation of lipid.
The specific embodiment
The present invention is further illustrated with specific embodiment below, but the present invention is not subjected to the restriction of following embodiment.
Embodiment 1.
A kind of black-tea fungus drink, by the raw material of following weight portion: 1000 parts in water, 100 parts of fermented tea bacterium liquid, 1 part of red date, 2 parts of longan, 5 parts of hawthorn, lichee is done 5 parts of 2 parts, black tea, 1 part of sea-buckthorn, 50 parts in rock sugar.
The preparation technology of this black-tea fungus drink, it is by following processing step:
(a) preparation of fermented tea bacterium liquid: the fermented tea bacterial classification that the market is buied is added in mother liquor and the black tea water stoste, and under 25 ℃ temperature, fermentation is 2 months in the clean environment, when the test pH value 3.0 below fermented tea bacterium liquid, filtration, standby;
(b) preliminary treatment of various raw materials: black tea is made black tea water through lixiviate, and heating refilters then, and is standby;
Red date, longan, hawthorn, lichee are done and sea-buckthorn is rinsed well, standby;
(c) mix: in joining cylinder, add pure water, pretreated red date, longan, hawthorn, lichee are done and sea-buckthorn among the fermented tea bacterium liquid for preparing among the adding step a and the step b, be heated to 100 ℃, heated 5 minutes, the black tea water of handling among the blend step b again, add rock sugar and mix, filter the back can;
(d) sterilization treatment: the black-tea fungus drink after the can was through 90 ℃ of pasteurizes 35 minutes.
Embodiment 2.
A kind of black-tea fungus drink, by the raw material of following weight portion: 1000 parts in water, 140 parts of fermented tea bacterium liquid, 3 parts of red dates, 1 part of longan, 3 parts of hawthorn, lichee is done 1 part, 6 parts of black teas, 5 parts of sea-buckthorns, 80 parts in fructose.
The preparation technology of this black-tea fungus drink, it has following processing step:
(a) preparation of fermented tea bacterium liquid: the fermented tea bacterial classification that the market is buied is added in mother liquor and the black tea water stoste, and under 35 ℃ temperature, fermentation is 3 months in the clean environment, when the test pH value 3.0 below fermented tea bacterium liquid, filtration, standby;
(b) preliminary treatment of various raw materials: black tea is made black tea water through lixiviate, and heating refilters then, and is standby;
Red date, longan, hawthorn, lichee are done and sea-buckthorn is rinsed well, standby;
(c) mix: in joining cylinder, add pure water, pretreated red date, longan, hawthorn, lichee are done and sea-buckthorn among the fermented tea bacterium liquid for preparing among the adding step a and the step b, be heated to 100 ℃, heated 20 minutes, the black tea water of handling among the blend step b again, add fructose and mix, filter the back can;
(d) sterilization treatment: the black-tea fungus drink after the can was through 85 ℃ of pasteurizes 45 minutes.
Embodiment 3.
A kind of black-tea fungus drink comprises the raw material of following weight portion: 1000 parts in water, and 250 parts of fermented tea bacterium liquid, 6 parts of red dates, 3 parts of longan, 4 parts of hawthorn, lichee is done 2 parts, 10 parts of black teas, 2 parts of sea-buckthorns, 100 parts of honey.
The preparation technology of this black-tea fungus drink, it comprises following processing step:
(a) preparation of fermented tea bacterium liquid: the fermented tea bacterial classification that the market is buied is added in mother liquor and the black tea water stoste, under 28 ℃ of temperature, in the clean environment fermentation 1 month, when the test pH value 3.0 below fermented tea bacterium liquid, the filtration, standby;
(b) preliminary treatment of various raw materials: black tea is prepared into black tea water through lixiviate, and heating refilters then, and is standby;
Red date, longan, hawthorn, lichee are done and sea-buckthorn is rinsed well, standby;
(c) mix: in joining cylinder, add pure water, adding among the fermented tea bacterium liquid for preparing among the step a and the step b after the preliminary treatment red date, longan, hawthorn, lichee does and sea-buckthorn, be heated to 100 ℃, heated 10 minutes, the black tea water of handling among the blend step b again, add the honey dissolving and mix, filter the back can;
(d) sterilization treatment: the black-tea fungus drink after the can was through 100 ℃ of pasteurizes 50 minutes.
Embodiment 4.
A kind of black-tea fungus drink comprises the raw material of following weight portion: 1000 parts in water, 200 parts of fermented tea bacterium liquid, 4 parts of red dates, 2 parts of longan, 2 parts of hawthorn, 5 parts of black teas, 80 parts of caramel.
The preparation technology of this black-tea fungus drink, it comprises following processing step:
(a) preparation of fermented tea bacterium liquid: the fermented tea bacterial classification that the market is buied is added in mother liquor and the black tea water stoste, in 29 ℃ of clean environments of temperature the fermentation 2 months, when the test pH value 3.0 below fermented tea bacterium liquid, the filtration, standby;
(b) preliminary treatment of various raw materials: black tea is prepared into black tea water through lixiviate, and heating refilters then, and is standby;
Red date, hawthorn, longan are rinsed well, standby;
(c) mix: in joining cylinder, add pure water, add among the fermented tea bacterium liquid for preparing among the step a and the step b after the preliminary treatment red date, hawthorn, lichee and do and sea-buckthorn, be heated to 100 ℃, heated 15 minutes, the black tea water of handling among the blend step b adds the caramel dissolving and mixes again, filters the back can;
(d) sterilization treatment: the black-tea fungus drink after the can was through 95 ℃ of pasteurizes 40 minutes.
Embodiment 5.
A kind of black-tea fungus drink comprises the raw material of following weight portion: 1000 parts in water, and 300 parts of fermented tea bacterium liquid, 10 parts of red dates, 5 parts of longan, 1 part of hawthorn, lichee is done 5 parts, 2 parts of black teas, 3 parts of sea-buckthorns, 200 parts of glucose.
The preparation technology of this black-tea fungus drink, it comprises following processing step:
(a) preparation of fermented tea bacterium liquid: the fermented tea bacterial classification that the market is buied is added in mother liquor and the black tea water stoste, and under 30 ℃ temperature, fermentation is 2 months in the clean environment, when the test pH value 3.0 below fermented tea bacterium liquid, filtration, standby;
(b) preliminary treatment of various raw materials: black tea is made black tea water through lixiviate, and heating refilters then, and is standby;
Red date, longan, hawthorn, lichee are done and sea-buckthorn is rinsed well, standby;
(c) mix: in joining cylinder, add pure water, pretreated red date, longan, hawthorn, lichee are done and sea-buckthorn among the fermented tea bacterium liquid for preparing among the adding step a and the step b, be heated to 100 ℃, heated 10 minutes, the black tea water of handling among the blend step b again, add glucose and mix, filter the back can;
(d) sterilization treatment: the black-tea fungus drink after the can was through 80 ℃ of pasteurizes 30 minutes.
The mechanism of action of building up resistance of the present invention is experimentized.
One, experiment material
(1) medicine and reagent: black-tea fungus drink of the present invention, every ml contains raw material 0.3g.Glutamic-pyruvic transaminase (SGPT) kit is buied by Shanghai inferior training bio tech ltd; Blood urea nitrogen (BUN) (BUN) kit is buied by the Shanghai hawk biological reagent Co., Ltd that jumps, the sulfuration barbiturates, and chemical reagent work buys by East China, Tianjin, and Sigma product and the equal market of all the other reagent fetch.
(2) animal: healthy Kunming mouse, body weight 20g ± 2g, male and female half and half are provided by Guangdong Medical Lab Animal Center.
Two, experiment and result
(1) to the influence of animal tolerance
Choose hundreds of of 20g ± 2g mouse, the galvanized wire that elder generation bears a heavy burden 10% body weight for every mouse afterbody, allow mouse in the water tank of 50cm * 30cm * 50cm, swim, get swimming time 60 of the mouse of 1~3min, be divided into 2 groups, 30 every group, organize in contrast for one group, irritate stomach physiological saline 0.5ml/10g, another is organized as experimental group, irritates stomach black-tea fungus drink 0.5ml/10g of the present invention, two groups of continuous weeks of mouse, dosage sees Table 1, irritates stomach for the last time after one hour, and mouse is put in the water tank, allow itself and the same swimming with a load attached to the body before, depth of water 20cm in the water tank, the record mouse is put into water tank and sinks to the bottom time of death to swimming time, compares.
Table 1 black-tea fungus drink of the present invention is to the influence of mouse load swimming time
Group Number of animals (only) Dosage Load swimming time (min)
Control group 30 0.5ml/10g 5.03±1.87
Experimental group 30 0.5ml/10g 10.32±2.61
As shown in Table 1, black-tea fungus drink of the present invention can obviously prolong mouse load swimming time, points out black-tea fungus drink of the present invention can strengthen the dynamic tolerance effect of animal.With the poor heteropole of control group remarkable (P<0.01), its rate elongation can reach 74.62%.
(2) to the influence of the humoral immune function of mouse
Choose 60 of kunming mices, be divided into 2 groups immediately, 30 every group, body weight 20g ± 2g, male and female half and half, experimental group continuous irrigation stomach black-tea fungus drink 0.5ml/10g of the present invention 14d, control group continuous irrigation stomach physiological saline 0.5ml/10g 14d.24h lumbar injection 0.2ml 5% chicken erythrocyte suspension carries out immunity before the experiment.Behind the 24h, the eye socket vein is got blood, gets serum 20 μ l for every, dilutes 100 times, 0 ℃ adds 5% chicken red blood cell and each 05.ml of 10% complement, after 370 ℃ of temperature are bathed 30min, and cessation reaction in 0 ℃ of refrigerator, centrifugal, in 721 spectrophotometer 540nm place colorimetrics, record OD value, relatively the difference between two groups sees Table 2.
Table 2 black-tea fungus drink of the present invention is to the influence of mouse humoral immune power function.
Group Number of animals (only) Dosage Serum hemolysin level (OD value)
Control group 30 0.5ml/10g 0.135±0.052
Experimental group 30 0.5ml/10g 0.54±0.079
As shown in Table 2, after black-tea fungus drink of the present invention is irritated stomach 14d, in the experimental group in the mice serum OD value be significantly higher than control group level (P<0.01), illustrate that black-tea fungus drink of the present invention can make the mice serum hemolysin level increase, and discloses the effect that black-tea fungus drink of the present invention has increases the animal body fluid immunologic function.
(3) anti peroxidation of lipid experiment
Choose 60 of kunming mices, random packet, 30 every group, body weight 20g ± 2g, male female half and half.Experimental group is irritated stomach black-tea fungus drink 0.5ml/10g of the present invention, control group is irritated stomach physiological saline 0.5ml/10g, the mouse administration was analysed after 14 days, get its heart, liver and brain, round a heart and brain and part liver weighing scale to test twisting force weighing, add physiological saline 5ml/ and carry out homogenate, every pipe adds trichloroacetic acid (20%) 0.5ml precipitating proteins after the homogenate, after 2000r/min was centrifugal, every pipe was got supernatant 1ml, add thiobarbituricacid 1ml after, place 15min in the waste water bath, take out back 520nm place colorimetric on 721 spectrophotometers, calculate every 50g heart, the OD value of 140g brain and 250d liver, what of mda content in the size of the OD value reflection solution, and MDA is one of end-product behind the lipid peroxidation, and its degree that how much can reflect lipid peroxidation indirectly the results are shown in Table 3.
Table 3 black-tea fungus drink of the present invention is to the influence of mouse anti lipid peroxidation
Figure 299114DEST_PATH_IMAGE002
As shown in Table 3, after black-tea fungus drink of the present invention is irritated stomach 14d, the OD value of the heart in the experimental group in the mouse, liver and brain significantly reduces (P<0.01), reflected the MDA content reduction, having disclosed black-tea fungus drink of the present invention has the obvious suppression effect to the lipid peroxidation product of the heart, liver and the brain of mouse, and then disclosing black-tea fungus drink of the present invention has stronger lipoid peroxidization resistant.
The above only is preferred embodiment of the present invention, so all equivalences of doing according to the described structure of patent claim of the present invention, feature and principle change or modify, is included in the patent claim of the present invention.

Claims (8)

1. black-tea fungus drink, it is characterized in that: the raw material by following weight portion is formed:
1000 parts in water
Fermented tea bacterium liquid 100-300 part
Red date 1-10 part
Longan 1-5 part
Hawthorn 1-5 part
Lichee is done 0-5 part
Black tea 2-10 part
Sea-buckthorn 0-5 part
Sweetener 50-200 part,
Wherein, described fermented tea bacterium liquid is the mixed liquor of fermented tea bacterial classification, mother liquor and black tea water stoste,
Wherein, described black tea water stoste is to be prepared from according to the weight portion ratio of 1-3:10:100 by tealeaves, sugar, water;
Wherein, described sweetener comprise in white sugar, rock sugar, fructose, honey, glucose, the caramel any one or more than one;
Wherein, it is prepared from by following preparation process:
(a) preparation of fermented tea bacterium liquid: the fermented tea bacterial classification that the market is buied is added in mother liquor and the black tea water stoste, under 25 ℃~35 ℃ temperature, fermented 1~3 month in the clean environment, when test pH value below 3.0 fermented tea bacterium liquid, filter, standby;
(b) preliminary treatment of various raw materials: black tea is made black tea water through lixiviate, and heating refilters then, and is standby;
Red date, lichee do, longan, hawthorn and sea-buckthorn rinse well, and be standby;
(c) mix: in joining cylinder, add pure water, add in the fermented tea bacterium liquid for preparing among the step a and the step (b) that pretreated red date, lichee are done, longan, hawthorn, sea-buckthorn, be heated to 100 ℃, heated 5~20 minutes, the black tea water of handling in the blend step (b) again, add sweetener and mix, filter the back can;
(d) sterilization treatment: the black-tea fungus drink after the can was through 80 ℃~100 ℃ pasteurizes 30~50 minutes.
2. a kind of black-tea fungus drink according to claim 1, it is characterized in that: the raw material by following weight portion is formed:
1000 parts in water
Fermented tea bacterium liquid 140-250 part
Red date 3-6 part
Longan 1-3 part
Hawthorn 1-3 part
Lichee is done 1-3 part
Black tea 4-6 part
Sea-buckthorn 1-3 part
Sweetener 100-150 part.
3. a kind of black-tea fungus drink according to claim 1, it is characterized in that: the raw material by following weight portion is formed:
1000 parts in water
200 parts of fermented tea bacterium liquid
4 parts of red dates
2 parts of longan
2 parts of hawthorn
Lichee is done 2 parts
5 parts of black teas
2 parts of sea-buckthorns
80 parts of sweeteners.
4. a kind of black-tea fungus drink according to claim 1, it is characterized in that: the bacterium liquid fermentation time in the described step (a) is 2 months.
5. a kind of black-tea fungus drink according to claim 1, it is characterized in that: be 10~15 minutes the heat time heating time in the described step (c).
6. a kind of black-tea fungus drink according to claim 1, it is characterized in that: the sterilising temp in the described step (d) is 85 ℃~95 ℃, and sterilizing time is 35~45 minutes.
7. a kind of black-tea fungus drink according to claim 1 is characterized in that: the red date in the described step (b), lichee are done, longan, hawthorn and sea-buckthorn are rinsed well, after waiting to do, are prepared into powdery, and be standby.
8. the application of any described a kind of black-tea fungus drink of claim 1-7 is characterized in that: in strengthening human body tolerance, resistance and anti peroxidation of lipid health-care effect is preferably arranged.
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CN104957304A (en) * 2015-07-10 2015-10-07 张铁英 Beautifying beverage containing black tea fungus and preparation method of beautifying beverage
CN104982564A (en) * 2015-07-21 2015-10-21 江苏华桑食品科技有限公司 Black tea fungus culturing method
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CN108770966A (en) * 2018-06-27 2018-11-09 中粮德信行健康产业有限公司 A kind of fermented tea drink with function and preparation method thereof
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TWI747121B (en) * 2019-05-21 2021-11-21 大江生醫股份有限公司 Use of fermented product of camellia sinensis leaves for regulating expressions of cct gene, pink1 gene, parp2 gene, mitf gene, ung gene, ercc6 gene, terc gene, cd40 gene, dynll2 gene, grk5 gene, relb gene, tnfsf14 gene, il4r gene, and rela gene, and anti-aging, whitening, enhancing immunity and reducing fat accumulation

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