[go: up one dir, main page]

CN102788882B - Method for detecting trace protein - Google Patents

Method for detecting trace protein Download PDF

Info

Publication number
CN102788882B
CN102788882B CN201210294634.3A CN201210294634A CN102788882B CN 102788882 B CN102788882 B CN 102788882B CN 201210294634 A CN201210294634 A CN 201210294634A CN 102788882 B CN102788882 B CN 102788882B
Authority
CN
China
Prior art keywords
antiserum
reaction
treating fluid
detection method
curve
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201210294634.3A
Other languages
Chinese (zh)
Other versions
CN102788882A (en
Inventor
郑焕巍
李华涛
陈亚球
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shenzhen Jinrui Biotechnology Co.,Ltd.
Original Assignee
SHENZHEN GENIUS ELECTRONICS CO Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SHENZHEN GENIUS ELECTRONICS CO Ltd filed Critical SHENZHEN GENIUS ELECTRONICS CO Ltd
Priority to CN201210294634.3A priority Critical patent/CN102788882B/en
Publication of CN102788882A publication Critical patent/CN102788882A/en
Application granted granted Critical
Publication of CN102788882B publication Critical patent/CN102788882B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention discloses a method for detecting trace proteins. The method comprises the following steps: preparing an antiserum treating fluid and a reaction buffer; after diluting the antiserum to 1-2 mg/ml by the antiserum treating fluid and then placing at room temperature, storing in an environment at 4 DEG C; adding the reaction buffer into a reaction container, and then adding a detection sample, and adding the antiserum after being processed by an antiserum treating process so as to form a reaction solution; detecting the reaction solution and reading response amplitude of the detection sample; and formulating a standard curve and carrying out nonlinear matching by a calibration solution with different concentrations. Based on the method for detecting the trace proteins, the treated antiserum is placed at the room temperature so that various components in the antiserum treating fluid and the antibodies in the antiserum are adequately acted, and the room temperature condition is relatively temperate to the antibodies, so that the antibodies and various components in the antiserum treating fluid are adequately acted; and meanwhile, the antiserum after being treated by the antiserum treating fluid and the treating method is good in stability, and the activity cannot be reduced after being placed for a year under a storage condition of 4 DEG C.

Description

Detection method for trace protein
Technical field
The present invention relates to clinical detection technique, particularly relate to a kind of detection method of trace of albumin.
Background technology
There is albumin in normal person's urine, but content is very low, is generally less than 30mg/24h urine.When kidney generation reversible lesion, the albumin in urine can increase, and generally in 30-300mg/24h urine, is now called microalbuminuria, and its mensuration is significant to the albuminuria of human body.
MALB(microdose urine protein due to used in the market) the concentration range that detects of detection method at about 10-220mg/L, the scope detected is partially narrow; Existing sero-fast processing mode causes sero-fast specificity strong not, and testing result affects greatly by the concentration of mALB in sample; The sample of clinical urine specimen, especially nephrotic, easily causes prozone phenomenon, forms the erroneous judgement of result; Another existing typical curve generally adopts fitting a straight line, this fit approach not science, causes the detected value of a certain section inaccurate.
Summary of the invention
Based on this, be necessary to provide a kind of detection method for trace protein that can keep antiserum activity the long period.
A kind of detection method for trace protein, is characterized in that, comprising:
Configuration damping fluid: configuration antiserum treating fluid, configuration reaction buffer;
Antiserum process: adopt antiserum treating fluid that antiserum is diluted to 1-2mg/ml, room temperature is placed 2-8 DEG C of temperature environment and preserved after placing;
Operation: add reaction buffer in reaction vessels, then adds detection sample, and adds the antiserum forming reactions solution after antiserum disposal methods;
Response analysis: tested reaction solution by protein analyzer, reads the range of reaction detecting sample;
Standard curve fit: utilize the calibration solution of variable concentrations to make typical curve, line nonlinearity matching of going forward side by side.
In a preferred embodiment, in described antiserum processing procedure, after adopting antiserum treating fluid that antiserum is diluted to 1-2mg/ml, room temperature is placed after 12-24 hour, then places 4 DEG C of refrigerator environment and preserves.
In a preferred embodiment, in operation, be further: in reaction vessels, add reaction buffer, then add detection sample, the reaction vessels adding detection sample is placed into the correspondence position of the reacting hole of protein analyzer, and adds the antiserum after process.
In a preferred embodiment, in described operation, the sero-fast volume ratio after the reaction buffer added, detection sample, process is 400:20:40; Response analysis is further: after the antiserum after protein analyzer senses process joins reaction vessels from reacting hole, automatically test, the range of reaction of read test sample.
In a preferred embodiment, in described operation, blank time is 6-8 second, and the reaction time is 122-124 second.
In a preferred embodiment, described protein analyzer adopts determined wavelength to be 650-690nm, and protein analyzer reads blank value according to blank time, reads range of reaction according to the reaction time.
In a preferred embodiment, described standard curve fit comprises further: the mALB calibration solution utilizing the RANDOX of variable concentrations, makes typical curve, to typical curve carry out non-linear, repeatedly, piecewise fitting.
In a preferred embodiment, described standard curve fit comprises further: detect steep lifting line section or curve, smoothing curve amendment.
In a preferred embodiment, described antiserum treating fluid: the Gly of the NaCl solution+40mM of the phosphate buffer+150mM of 20mM, PH7.0-7.8.
In a preferred embodiment, the reaction buffer of described configuration: the isinglass of the NaCl solution+0.01-0.5 mass percent of the phosphate buffer+30-100mM of 20mM, PH7.0-7.8.
The detection method of above-mentioned trace of albumin, adopt the antiserum after the process of antiserum treating fluid, adopt to place and process at room temperature, various component in antiserum treating fluid and the antibody in antiserum are fully acted on, because the condition of room temperature is relatively gentle for condition antibody, for antiserum treating fluid various components temperature be unlikely to too low, thus be conducive to the abundant effect of various component in antibody and antiserum treating fluid; Due to the abundant effect of various component in antibody and antiserum treating fluid, to make antiserum have good stability, place 1 year under 2-8 DEG C of condition of storage, activity can not reduce.
Accompanying drawing explanation
Fig. 1 is the range of linearity figure of one embodiment of the invention;
Fig. 2 is the data error line in the sensitivity test of table 6 in the present invention.
Embodiment
The detection method for trace protein of one embodiment of the invention, comprises the steps:
Configuration damping fluid: configuration antiserum treating fluid, configuration reaction buffer;
Antiserum process: adopt antiserum treating fluid that antiserum is diluted to 1-2mg/ml, room temperature is placed after 12-24 hour, to make the various component in antiserum treating fluid and the antibody in antiserum fully act on, then places 2 DEG C of-8 DEG C of temperature environments and preserves.Preferred further, Conservation environment is the temperature environment of 4 DEG C.
Operation: add reaction buffer in reaction vessels, then adds detection sample, and adds the antiserum after antiserum processing reaction;
Response analysis: carry out test reading by protein analyzer and get range of reaction;
Confirmed standard curve: the calibration solution confirmed standard curve utilizing variable concentrations, and nonlinear fitting is carried out to typical curve.
Preferably, adopt antiserum treating fluid that antiserum is diluted to 1-2mg/ml, room temperature was placed after 12-24 hour, placed in the refrigerator of 4 DEG C and preserved.Antiserum is diluted to 1-2mg/ml by antiserum treating fluid, room temperature is adopted to place process to make the various component in antiserum treating fluid and the antibody in antiserum fully act on, because the condition of room temperature is relatively gentle for condition antibody, for antiserum treating fluid various components temperature be unlikely to too low, thus be conducive to the abundant effect of various component in antibody and antiserum treating fluid.
Preferably, in the present embodiment, antiserum treating fluid: the Gly of the NaCl solution+40mM of the phosphate buffer+150mM of 20mM, PH7.0-7.8.
Preferably, in the present embodiment, reaction buffer: the isinglass of the NaCl solution+0.01-0.5 mass percent of the phosphate buffer+30-100mM of 20mM, PH7.0-7.8.
Further, in operation, the sero-fast volume ratio after reaction buffer, detection sample, antiserum processing reaction is 400:20:40.
The concrete operations of the operation in one embodiment of the present invention are as follows: add 400 microlitre reaction buffers at reaction vessels (as in reaction cup); The detection sample (as urine specimen) of 20 microlitres is added in the reaction cup that damping fluid is housed; Detect the reaction cup of sample be put into adding in the reacting hole of protein analyzer, and the antiserum after the antiserum added after the process of 40 microlitres and the process of antiserum treatment step.
Further, in operation, blank time preferably 6-8 second, preferably 122-124 second in reaction time.
In response analysis, when reaction cup is placed in the reacting hole of protein analyzer, after the antiserum after protein analyzer detects 40 microlitre process adds from reacting hole, protein analyzer is tested automatically, reads the range of reaction of test sample book.
In the present embodiment, antiserum preferably, ADVY company of India produce antiserum.
Further, the determined wavelength that protein analyzer adopts, preferably with antigen in reaction solution, that antibody response combines the particle size diameter formed is identical or close.Preferably, protein analyzer adopts determined wavelength to be 650-690nm.
Further, protein analyzer is according to the blank time reading blank value of setting, the range of reaction according to the reaction time read test sample arranged.
Further, the standard curve fit of the present embodiment comprises: the mALB calibration solution utilizing the RANDOX of variable concentrations, makes typical curve.Due to W-response trend, be non-linear, in the present embodiment, to typical curve adopt fitting software carry out non-linear, repeatedly, piecewise fitting.The range of linearity according to the detection of the typical curve after matching can reach 5-330mg/L.
The ideal response cohesive process of antigen and antibody is a level and smooth curve, but due to the impact of various factors, in the course of reaction of reality, a certain section of phenomenon suddenly raised appears in the curve occasional of antigen and antibody response, thus can affect testing result.Preferably, in the present embodiment to typical curve carry out non-linear, repeatedly, piecewise fitting process is further: detect steep lifting line section or curve, to this line segment or the smoothing transient curve correction of curve.Thus eliminate this impact to greatest extent, make the curve of matching more accurate, testing result is also more reliable.
Preferred embodiment 1:
The detection method for trace protein of the preferred embodiment of the present invention 1, comprises the steps:
Configuration damping fluid: configuration antiserum treating fluid: the Gly of the NaCl solution+40mM of the phosphate buffer+150mM of 20mM, PH7.0-7.8.
Configuration reaction buffer: the isinglass of the NaCl solution+0.01-0.5 mass percent of the phosphate buffer+30-100mM of 20mM, PH7.0-7.8.
Antiserum process: adopt antiserum treating fluid that antiserum is diluted to 1mg/ml, after room temperature places 12 hours, to make the various component in antiserum treating fluid and the antibody in antiserum fully act on, then places in the refrigerator of 4 DEG C of temperature and preserves.In the present embodiment, antiserum preferably, ADVY company of India produce antiserum.
Reaction time is arranged: arranging blank time is 6 seconds, and the reaction time is 122 seconds.
Operation: add 400 microlitre reaction buffers in reaction cup, then the urine specimen of 20 microlitres is added, the reaction cup of the urine specimen added is put in the reacting hole of protein analyzer, and antiserum after adding 40 microlitre reactions after the process of antiserum processing procedure.
Response analysis: after the antiserum after protein analyzer senses process adds, automatically test, reads blank value according to the blank time arranged, and reads range of reaction according to the reaction time arranged.
In the present embodiment, the protein analyzer that protein analyzer preferably selects Shenzhen Genius Electronics Co., Ltd. to manufacture.Preferably, protein analyzer selects wavelength to be the detection ripple of 650nm.
Confirmed standard curve: utilize the mALB calibration solution of variable concentrations RANDOX to make typical curve, adopt standard curve fitting software and to typical curve adopt non-linear, repeatedly, piecewise fitting, can 5-330mg/L be reached to make the detection range of linearity.
Preferred embodiment 2:
The detection method for trace protein of the preferred embodiment of the present invention 2, comprises the steps:
Configuration damping fluid: configuration antiserum treating fluid: the Gly of the NaCl solution+40mM of the phosphate buffer+150mM of 20mM, PH7.0-7.8.
Configuration reaction buffer: the isinglass of the NaCl solution+0.01-0.5 mass percent of the phosphate buffer+30-100mM of 20mM, PH7.0-7.8.
Antiserum process: adopt antiserum treating fluid that antiserum is diluted to 2mg/ml, after room temperature places 24 hours, to make the various component in antiserum treating fluid and the antibody in antiserum fully act on, then places in the refrigerator of 4 DEG C of temperature and preserves.In the present embodiment, antiserum preferably, ADVY company of India produce antiserum.
Reaction time is arranged: arranging blank time is 8 seconds, and the reaction time is 124 seconds.
Operation: add 400 microlitre reaction buffers in reaction cup, then the urine specimen of 20 microlitres is added, the reaction cup of the urine specimen added is put in the reacting hole of protein analyzer, and antiserum after adding 40 microlitre reactions after the process of antiserum processing procedure.
Response analysis: after the antiserum after protein analyzer senses process adds, automatically test, reads blank value according to the blank time arranged, and reads range of reaction according to the reaction time arranged.
In the present embodiment, the protein analyzer that protein analyzer preferably selects Shenzhen Genius Electronics Co., Ltd. to manufacture.Preferably, protein analyzer selects wavelength to be the detection ripple of 690nm.
Confirmed standard curve: utilize the mALB calibration solution of variable concentrations RANDOX to make typical curve, adopt fitting software or program and to typical curve adopt non-linear, repeatedly, piecewise fitting, can 5-330mg/L be reached to make the detection range of linearity.
Further, adopt fitting software or program and to typical curve adopt non-linear, repeatedly, piecewise fitting is specially the section of skyrocketing in detectable antigens and antibody response process, curve occurred, adopt to seamlessly transit curve and carry out auto modification.
Table 1: the range of linearity (antiserum preferably India ADVY company is produced) of detection
In the present embodiment, experiment is compared to the sero-fast range of reaction of different dilute concentration:
Table 2: the range of reaction of different antiserum dilute concentration compares
In antagonistic Serum processing procedure, the range of reaction after the antiserum after dilution adopts different disposal time (room temperature standing time) process is compared:
Table 3: the comparison of antiserum different disposal time response amplitude
Stability is tested:
By the antiserum after process 2 DEG C-8 DEG C, in the light protected environment of non-corrosiveness gas storage after 12 months, get the pattern detection of effect after date, analyze the relative deviation situation with target value.Test the antiserum after process with the mALB calibration solution of RANDOX, duplicate detection 10 times, takes the mean, and then calculates the relative deviation with target value.
Form 4-1: stability test
Form 4-2: stability
Accuracy measures: under repeated condition, test the reaction solution after operation process with the mALB calibration solution of RANDOX, replication ten times, then calculates inaccuracy and inaccuracy.
Form 5: inaccuracy and inaccuracy tables of data
Sensitivity behaviour is tested: be the reference material of 5mg/L with the target value of RANDOX, tests, replication ten times to the reaction solution after operation process, calculating variance.Then calculate the scope of the variance of target value plus-minus twice, and all values measured for ten times is all in scope.
Form 6: sensitivity
By table 1,2,3,4-2,4-2,5,6 and Fig. 1 and Fig. 2 can draw:
The detection method of trace of albumin of the present invention, the detection of particularly suitable microdose urine protein, by improving the processing mode of antibody, reaction time, the component of reaction buffer, the fit approach of typical curve, effectively alleviate prozone phenomenon, make that mALB sensing range is wider can reach 5-330mg/L, the accuracy detected improves greatly, thus is more conducive to clinical diagnosis.
Meanwhile, the antiserum after the present embodiment antiserum treating fluid and disposal methods has good stability, and place 1 year under 4 DEG C of conditions of storage, activity can not reduce.
Antibody property indices is given prominence to: accuracy: inaccuracy≤5% in batch; Inaccuracy≤6% between batch; Inaccuracy≤3%; Sensitivity: the mALB concentration that can detect 5mg/L, and the concentration value measured is all in ± 2SD.
The above embodiment only have expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.

Claims (8)

1. a detection method for trace protein, is characterized in that, comprising:
Configuration damping fluid: configuration antiserum treating fluid, configuration reaction buffer; Described antiserum treating fluid: the Gly of the NaCl solution+40mM of the phosphate buffer+150mM of 20mM, PH7.0-7.8; Described reaction buffer: the isinglass of the NaCl solution+0.01-0.5 mass percent of the phosphate buffer+30-100mM of 20mM, PH7.0-7.8;
Antiserum process: adopt antiserum treating fluid that antiserum is diluted to 1-2mg/ml, room temperature was placed after 12-24 hour, placed 2-8 DEG C of temperature environment and preserved;
Operation: add reaction buffer in reaction vessels, then adds detection sample, and adds the antiserum forming reactions solution after antiserum disposal methods;
Response analysis: tested reaction solution by protein analyzer, reads the range of reaction detecting sample;
Standard curve fit: utilize the calibration solution of variable concentrations to make typical curve, and nonlinear fitting is carried out to typical curve.
2. detection method for trace protein according to claim 1, it is characterized in that: in described antiserum processing procedure, after adopting antiserum treating fluid that antiserum is diluted to 1-2mg/ml, room temperature is placed after 12-24 hour, then places 4 DEG C of refrigerator environment and preserves.
3. detection method for trace protein according to claim 1, it is characterized in that: in operation, be further: in reaction vessels, add reaction buffer, then detection sample is added, the reaction vessels adding detection sample is placed into the correspondence position of the reacting hole of protein analyzer, and adds the antiserum after process.
4. detection method for trace protein according to claim 3, is characterized in that: in described operation, and the sero-fast volume ratio after the reaction buffer added, detection sample, process is 400:20:40; Response analysis is further: after the antiserum after protein analyzer senses process joins reaction vessels from reacting hole, automatically test, the range of reaction of read test sample.
5. the detection method for trace protein according to Claims 1-4 any one, is characterized in that: in described operation, and blank time is 6-8 second, and the reaction time is 122-124 second.
6. the detection method for trace protein according to Claims 1-4 any one, is characterized in that: described protein analyzer adopts determined wavelength to be 650-690nm, and protein analyzer reads blank value according to blank time, reads range of reaction according to the reaction time.
7. the detection method for trace protein according to Claims 1-4 any one, it is characterized in that: describedly nonlinear fitting is carried out to typical curve comprise further: the mALB calibration solution utilizing the RANDOX of variable concentrations, make typical curve, to typical curve carry out non-linear, repeatedly, piecewise fitting.
8. the detection method for trace protein according to Claims 1-4 any one, is characterized in that: describedly carry out nonlinear fitting to typical curve and comprise further: detect steep lifting line section or curve, smoothing curve amendment.
CN201210294634.3A 2012-08-17 2012-08-17 Method for detecting trace protein Active CN102788882B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201210294634.3A CN102788882B (en) 2012-08-17 2012-08-17 Method for detecting trace protein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201210294634.3A CN102788882B (en) 2012-08-17 2012-08-17 Method for detecting trace protein

Publications (2)

Publication Number Publication Date
CN102788882A CN102788882A (en) 2012-11-21
CN102788882B true CN102788882B (en) 2014-12-17

Family

ID=47154339

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201210294634.3A Active CN102788882B (en) 2012-08-17 2012-08-17 Method for detecting trace protein

Country Status (1)

Country Link
CN (1) CN102788882B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104750132B (en) * 2015-04-21 2017-03-15 三诺生物传感股份有限公司 A kind of test temperature bearing calibration, controller and test temperature correction system
CN104991056B (en) * 2015-08-05 2017-01-04 武汉林勉生物技术有限公司 A kind of Serologic detection and the method for quantitative analysis
JP6805272B2 (en) * 2016-06-15 2020-12-23 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft Improved D-dimer assay calibration

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1576844A (en) * 2003-07-09 2005-02-09 松下电器产业株式会社 Turbidimetric immunoassay and an apparatus therefor
CN101055272A (en) * 2006-04-13 2007-10-17 奥林巴斯生命及材料科学欧洲有限公司 Immunoassay method
CN101793893A (en) * 2009-02-03 2010-08-04 王武康 Direct competitive enzyme-linked immunoassay kit for detecting gentamicin

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7662578B2 (en) * 2006-04-21 2010-02-16 Children's Hospital Medical Center Method and kit for the early detection of impaired renal status

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1576844A (en) * 2003-07-09 2005-02-09 松下电器产业株式会社 Turbidimetric immunoassay and an apparatus therefor
CN101055272A (en) * 2006-04-13 2007-10-17 奥林巴斯生命及材料科学欧洲有限公司 Immunoassay method
CN101793893A (en) * 2009-02-03 2010-08-04 王武康 Direct competitive enzyme-linked immunoassay kit for detecting gentamicin

Also Published As

Publication number Publication date
CN102788882A (en) 2012-11-21

Similar Documents

Publication Publication Date Title
CN104198732B (en) A kind of neutrophil gelatinase-associated lipocalin reagent box for detecting content
CN103604930B (en) A kind of lipoprotein (a) detection kit
CN101881777B (en) Assay method of high sensitivity C-reactive protein (HS-CRP) and HS-CRP assay kit
CN111337691B (en) Sensitive and stable serum procalcitonin determination kit and preparation method and application thereof
CN107942067B (en) Cyclic citrullinated peptid assay kit
CN104237522A (en) Adiponectin content detection kit and preparation method thereof
CN105510604A (en) Method for improving sensitivity and linearity of latex reagent
CN103823070A (en) Cystatin C determination kit with high sensitivity
CN104198723A (en) Rapid NGAL (Neutrophil Gelatinase Associated Lipocalin) detection kit based on amino acid spacer arm
CN105137089B (en) Serum ferritin content detection kit
CN104614528A (en) Wider linear range retinol binding protein determination kit
CN102628802B (en) Method for detecting biotoxins in foods based on surface plasma resonance technology
CN105137082A (en) Dual-reagent glycosylated hemoglobin detection kit
CN102788882B (en) Method for detecting trace protein
CN108680622A (en) Packed cell volume measures and the method for correction in a kind of electrochemica biological sensor
CN104849473A (en) Microalbuminuria detection kit and preparation thereof
CN104833809A (en) Latex-enhanced immunonephelometry kit for determination of resistin, preparation method and detection method thereof
CN105548558B (en) A method, device and kit for detecting H factor concentration
CN106771152A (en) A kind of kit of quick detection calprotectin
Palosuo et al. Latex allergy: the sum quantity of four major allergens shows the allergenic potential of medical gloves
CN105403712A (en) High performance detection kit for human urine alpha 1 acidoglycoprotein
CN102879569A (en) Immunoturbidimetry kit for detecting cysteine proteinase inhibitor C and preparation method thereof
CN107942068B (en) β2Microglobulin assay kit
Khatami et al. Measurement verification in the clinical laboratory: a guide to assessing analytical performance during the acceptance testing of methods (quantitative examination procedures) and/or analysers
CN113156136B (en) Kit for detecting immunoglobulin G in urine

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
C56 Change in the name or address of the patentee
CP03 Change of name, title or address

Address after: Nanshan District Taoyuan street 518000 Guangdong city of Shenzhen Province Liu Xian Da Dao Nan Shan Yungu Innovation Industrial Park 1183 building 6 floor B landscape

Patentee after: SHENZHEN GENRUI BIOTECHNOLOGY CO., LTD.

Address before: 518067, C, building 6, Shekou seven industrial road, Shekou, Guangdong, Nanshan District, Shenzhen

Patentee before: Shenzhen Genius Electronics Co., Ltd.

CP01 Change in the name or title of a patent holder
CP01 Change in the name or title of a patent holder

Address after: 518000 B, 6th floor, Shanshui building, Nanshan cloud Valley Innovation Industrial Park, 1183 Liuxian Avenue, Taoyuan Street, Nanshan District, Shenzhen City, Guangdong Province

Patentee after: Shenzhen Jinrui Biotechnology Co.,Ltd.

Address before: 518000 B, 6th floor, Shanshui building, Nanshan cloud Valley Innovation Industrial Park, 1183 Liuxian Avenue, Taoyuan Street, Nanshan District, Shenzhen City, Guangdong Province

Patentee before: GENRUI BIOTECH Inc.