CN102766172A - Industrial production method of rhamnolipid biosurfactant dry powder - Google Patents
Industrial production method of rhamnolipid biosurfactant dry powder Download PDFInfo
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- CN102766172A CN102766172A CN2011102774253A CN201110277425A CN102766172A CN 102766172 A CN102766172 A CN 102766172A CN 2011102774253 A CN2011102774253 A CN 2011102774253A CN 201110277425 A CN201110277425 A CN 201110277425A CN 102766172 A CN102766172 A CN 102766172A
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- rhamnolipid
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- FCBUKWWQSZQDDI-UHFFFAOYSA-N rhamnolipid Chemical compound CCCCCCCC(CC(O)=O)OC(=O)CC(CCCCCCC)OC1OC(C)C(O)C(O)C1OC1C(O)C(O)C(O)C(C)O1 FCBUKWWQSZQDDI-UHFFFAOYSA-N 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 24
- 239000000843 powder Substances 0.000 title claims abstract description 16
- 239000003876 biosurfactant Substances 0.000 title claims abstract description 5
- 238000009776 industrial production Methods 0.000 title abstract 2
- 238000001914 filtration Methods 0.000 claims abstract description 12
- 238000001694 spray drying Methods 0.000 claims abstract description 5
- 238000000703 high-speed centrifugation Methods 0.000 claims abstract description 3
- 238000000926 separation method Methods 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 30
- 238000005507 spraying Methods 0.000 claims description 11
- 239000000126 substance Substances 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 9
- 239000002131 composite material Substances 0.000 claims description 8
- 238000000855 fermentation Methods 0.000 claims description 7
- 230000004151 fermentation Effects 0.000 claims description 7
- 230000003311 flocculating effect Effects 0.000 claims description 6
- 235000013826 starch sodium octenyl succinate Nutrition 0.000 claims description 6
- 239000001334 starch sodium octenyl succinate Substances 0.000 claims description 6
- 238000011085 pressure filtration Methods 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 3
- 239000010409 thin film Substances 0.000 claims description 3
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 claims description 2
- 229930186217 Glycolipid Natural products 0.000 claims description 2
- 239000007921 spray Substances 0.000 claims description 2
- 238000003860 storage Methods 0.000 abstract description 12
- 239000000047 product Substances 0.000 description 10
- 238000004519 manufacturing process Methods 0.000 description 7
- 239000013543 active substance Substances 0.000 description 5
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 239000012190 activator Substances 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000004480 active ingredient Substances 0.000 description 2
- 238000004737 colorimetric analysis Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 229910052697 platinum Inorganic materials 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000010792 warming Methods 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000010408 film Substances 0.000 description 1
- 125000001165 hydrophobic group Chemical group 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000012263 liquid product Substances 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 229940023462 paste product Drugs 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to an industrial production method of a rhamnolipid biosurfactant dry powder, comprising the following steps of: (1) coarse filtration; (2) high-speed centrifugation; (3) vacuum concentration; (4) addition of an embedding medium; (5) spray drying; and (6) cyclone separation. The dry powder rhamnolipid produced by the method is convenient to store and transport, and during the storage process, active components will not be minimized. Simultaneously, application field is widened.
Description
Technical field:
The present invention relates to a kind of industrialized preparing process of rhamnolipid biological surface activator dry powder.
Background technology:
Tensio-active agent is meant a kind of material that possesses a fixed structure, lypohydrophilic character is arranged that can significantly reduce surface tension and IT.Mainly be divided into chemical classes tensio-active agent and biological species tensio-active agent, the most frequently used is the chemical classes tensio-active agent, but generally has poisonous, harmful in its production process, use and have the situation of pollution to produce to environment.Compare, the biological species tensio-active agent mainly is to utilize the physiological metabolism of mikrobe to produce, and it has outside the surface of good activity, and also possessing has structure diversity, low toxicity or nontoxic, and biodegradable adapts to characteristics such as extreme temperature, pH and salinity.Its production process and use can not produce harm and environmental pollution.It has bigger research and using value at numerous areas such as oil production, environmental protection, medicine, daily use chemicals, food.
Rhamnolipid is a kind of bio-surfactant that is produced under certain condition by the green pseudomonas of ketone; It is characterized in that integrating hydrophilic group and hydrophobic group structure; Compare with chemical surfactant that rhamnolipid is nontoxic, degradable, good biocompatibility, therefore have the potential using value in food, environment, oil production, soil remediation, daily use chemicals, medicine and other fields.Rhamnolipid can carry out suitability for industrialized production at present, but the form of its fermented liquid can not be used as the finished product.Major cause is following: 1, be unfavorable for storing: conventional condition of storage can make fermented liquid smelly in a short time rotten and influence is used, and also can make active ingredient reduce 5-10% through the shelf time more than 1 month.2, do not utilize transportation: the one, liquid form itself is inconvenient to transport; The 2nd, produce the fat amount by the highest present industriallization and calculate; Wherein the content of the pure article of rhamnolipid is about 5%, and all the other invalid elements of about 95% have increased the cost of transportation greatly in transportation.In addition, liquid rhamnolipid is unfavorable for the application in follow-up field: the rhamnolipid product can not use liquid product in some Application Areas, limited development of market.Such as, be used for paint additive, powdery and granulated fertilizer, sewage treatment area, daily use chemicals and field of food etc.
Summary of the invention:
The purpose of this invention is to provide a kind of method that is applicable to commercial scale prodn rhamnolipid biological surface activator dry powder.The dry powder rhamnolipid of being produced by present method is beneficial to storages, utilize transport and in storage process active ingredient can not reduce, widened Application Areas simultaneously.
The technical scheme that the present invention adopted is: the industrialized preparing process of this mouse glycolipid bio-surfactant dry powder, and the method includes the steps of:
(1) coarse filtration: the industriallization rhamnolipid fermentation liquor stoste that zymotechnique is produced is added flocculating aids; Addition is 0.5%～1% of a rhamnolipid fermentation liquor gross weight; Use plate-and-frame filter press under 50 ℃～60 ℃ temperature condition, to carry out pressure filtration, pressure 0.1～0.2Mpa removes solid substance; Flocculating aids is available from the permanent all flocculating aids ltds of Meihekou, and model is 300#.
(2) high speed centrifugation: the fermented liquid through coarse filtration carries out centrifugal removal thalline, centrifugal speed 8000～10000rpm/min through dish formula supercentrifuge;
(3) concentrating under reduced pressure: the fermented liquid that will go on foot (2) centrifugal mistake carries out concentrating under reduced pressure through thin-film evaporator; The concentrating under reduced pressure process keep-up pressure into-0.05Mpa～-0.08Mpa, 60 ℃～70 ℃ of temperature make that rhamnolipid concentration reaches 180g/L～200g/L in the liquid concentrator;
(4) add embedding medium: the liquid concentrator of step (3) is collected into composite jar, by 1～3% (W/W) the interpolation starch Sodium Octenyl Succinate of pure rhamnolipid content, composite evenly subsequent use;
(5) spraying drying: the compositional liquor of step (4) is squeezed into centrifugal spray drying tower (the design steam output is 150l/h) through take away pump carry out spraying drying; Spray process adopts 6000m
3The blast volume of/h, EAT are controlled at 140 ℃～160 ℃, and air outlet temperature is controlled at 75 ℃;
(6) cyclonic separation: the pulvis after the spraying drying separates through the two stage cyclone separator, collects the dry powder finished product in the separator bottom.
Technique effect of the present invention is: the present invention does not adopt methods such as extraction, filtration to purify to separate and purifying; Occur with the paste form; Because: 1, its subsequent extracted, stripping technique are difficult to realize in suitability for industrialized production; Can use deleterious organic solvent in the leaching process, and increase the cost of the finished product greatly; 2, the paste product after the purification is soluble in organic solvent and is difficult to water-soluble problem, and the application of rhamnolipid in a lot of fields that the extraction of this kind method is obtained lost value.The rhamnolipid industry dry powder of producing through this technology spraying drying can reach the purity (W/W) of 60%-80%, and pink colour is pure white to faint yellow, and opaque is fine and smooth fluffy non-caked, soluble in water, and convenient production is convenient to store, transport and the application of some industry.The minimum 31.65mN/m (CMC46.25mg/L) that reaches of surface tension, identical with the rhamnolipid fermentation liquor surface tension, its surfactivity does not change.Quality guaranteed period can reach more than 2 years.
Embodiment:
Through embodiment the present invention is specifically described below:
Embodiment 1:
In 20t rhamnolipid fermentation liquor (30g/L), add 300# flocculating aids 100kg, stir the 20min mixing, be warming up to 55 ℃ (are retained to filter and accomplish) simultaneously.In the fermented liquid storage tank, feed pressurized air, pressure is 0.1Mpa, uses plate-and-frame filter press XMAY60/920 type to carry out pressure filtration, removes solid substance, and the fermented liquid after the filtration is preserved by independent storage tank.
After filtering completion, through product pump, the fermented liquid after filtering is delivered to supercentrifuge, DHC/DRY-400 carries out centrifugal removal thalline through dish formula supercentrifuge, and centrifugal rotational speed is set at 8000rpm/min, carries out the continuously centrifuged operation.
Through the centrifugal fermented liquid, be delivered to wiped film evaporator (steam output 15m through pipeline
2) carry out concentrating under reduced pressure.The adjustment working pressure be-0.06Mpa about, temperature remains on 60 ℃-70 ℃, makes that rhamnolipid concentration reaches 180g/L in the final liquid concentrator, the fermented liquid after concentrated is stored in the liquid concentrator storage tank subsequent use.
The 16kg starch Sodium Octenyl Succinate (SSOS) of in the liquid concentrator storage tank, adding stirs 20min, and composite even back is subsequent use.
Liquid concentrator after composite is squeezed into centrifugal spray drying tower through take away pump carry out spraying drying.The spraying gun speed setting is 8000rpm/min, and blast volume is adjusted into 6000m
3/ h, control EAT make air outlet temperature be controlled at 70 ℃-80 ℃ through the adjustment inlet amount about 145 ℃.
Pulvis separates through the two stage cyclone separator, collects the dry powder finished product in the separator bottom.Finished product is adopted 3, and 5-orcin-sulfuric acid colourimetry is measured, and instrument uses the TU-1810S ultraviolet spectrophotometer, and wavelength 421 is done typical curve with the rhamnolipid standard specimen.Through measuring pure the crossing of rhamnolipid dry powder is 62% (W/W), and pink colour is pure white, and opaque is fine and smooth fluffy non-caked.Rhamnolipid active (surface tension) is measured: adopt platinum plate method to measure, use instrument to be the full-automatic surface tension instrument of QBZV.The minimum 31.65mN/m of reaching of the surface tension that records.
Embodiment 2:
In 10t rhamnolipid fermentation liquor (40g/L), add 300# flocculating aids 50kg, stir the 20min mixing, be warming up to 60 ℃ (are retained to filter and accomplish) simultaneously.In the fermented liquid storage tank, feed pressurized air, pressure is 0.15mpa, carries out pressure filtration, removes solid substance, and the fermented liquid after the filtration is preserved by independent storage tank.
After filtering completion, through product pump, the fermented liquid after filtering is delivered to supercentrifuge, centrifugal rotational speed is set at 8000rpm/min, carries out the continuously centrifuged operation.
Through the centrifugal fermented liquid, be delivered to thin-film evaporator through pipeline, carry out concentrating under reduced pressure.The adjustment working pressure be-0.05Mpa about, temperature remains on 60 ℃-70 ℃, makes that rhamnolipid concentration reaches 190g/L in the final liquid concentrator, the fermented liquid after concentrated is stored in the liquid concentrator storage tank subsequent use.
The 4kg starch Sodium Octenyl Succinate (SSOS) of in the liquid concentrator storage tank, adding stirs 20min, and composite even back is subsequent use.
Liquid concentrator after composite is squeezed into centrifugal spray drying tower through take away pump carry out spraying drying.The spraying gun speed setting is 10000rpm/min, and blast volume is adjusted into 6000m
3/ h, control EAT are controlled at about 75 ℃ air outlet temperature through the adjustment inlet amount about 150 ℃.
Pulvis separates through the two stage cyclone separator, collects the dry powder finished product in the separator bottom.Finished product is adopted 3, and 5-orcin-sulfuric acid colourimetry is measured, and instrument uses the TU-1810S ultraviolet spectrophotometer, and wavelength 421 is done typical curve with the rhamnolipid standard specimen.Through measuring pure the crossing of rhamnolipid dry powder is 75% (W/W), and pink colour is yellowish, and opaque is fine and smooth fluffy non-caked.Rhamnolipid active (surface tension) is measured: adopt platinum plate method to measure, use instrument to be the full-automatic surface tension instrument of QBZV.The minimum 31.65mN/m of reaching of the surface tension that records.
Claims (1)
1. the industrialized preparing process of a mouse glycolipid bio-surfactant dry powder, the method includes the steps of:
(1) coarse filtration: the industriallization rhamnolipid fermentation liquor stoste that zymotechnique is produced is added flocculating aids; Addition is 0.5%～1% of a rhamnolipid fermentation liquor gross weight; Use plate-and-frame filter press under 50 ℃～60 ℃ temperature condition, to carry out pressure filtration, pressure 0.1～0.2Mpa removes solid substance;
(2) high speed centrifugation: the fermented liquid through coarse filtration carries out centrifugal removal thalline, centrifugal speed 8000～10000rpm/min through dish formula supercentrifuge;
(3) concentrating under reduced pressure: the fermented liquid that will go on foot (2) centrifugal mistake carries out concentrating under reduced pressure through thin-film evaporator; The concentrating under reduced pressure process keep-up pressure into-0.05Mpa～-0.08Mpa, 60 ℃～70 ℃ of temperature make that rhamnolipid concentration reaches 180g/L～200g/L in the liquid concentrator;
(4) add embedding medium: the liquid concentrator of step (3) is collected into composite jar, by 1～3% (W/W) the interpolation starch Sodium Octenyl Succinate of pure rhamnolipid content, composite evenly subsequent use;
(5) spraying drying: the compositional liquor of step (4) is squeezed into centrifugal spray drying tower through take away pump carry out spraying drying; Spray process adopts 6000m
3The blast volume of/h, EAT are controlled at 140 ℃～160 ℃, and air outlet temperature is controlled at 75 ℃;
(6) cyclonic separation: the pulvis after the spraying drying separates through the two stage cyclone separator, collects the dry powder finished product in the separator bottom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102774253A CN102766172A (en) | 2011-09-19 | 2011-09-19 | Industrial production method of rhamnolipid biosurfactant dry powder |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011102774253A CN102766172A (en) | 2011-09-19 | 2011-09-19 | Industrial production method of rhamnolipid biosurfactant dry powder |
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| CN102766172A true CN102766172A (en) | 2012-11-07 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016115048A1 (en) | 2015-01-12 | 2016-07-21 | Logos Technologies, Llc | Production of rhamnolipid compositions |
| US9884883B2 (en) | 2015-01-12 | 2018-02-06 | Logos Technologies, Llc | Production of rhamnolipid compositions |
| CN107937273A (en) * | 2017-11-22 | 2018-04-20 | 北京沃太斯环保科技发展有限公司 | Rhamnolipid is used as protectant application in microniological proudcts spray-drying process |
| CN111205844A (en) * | 2020-03-09 | 2020-05-29 | 陕西斯普曼生物工程有限公司 | Treatment method of rhamnolipid fermentation liquor of oil field oil displacement agent |
| US10829507B2 (en) | 2017-02-06 | 2020-11-10 | Stepan Company | Decolorization of concentrated rhamnolipid composition |
| CN112108075A (en) * | 2020-08-19 | 2020-12-22 | 山东将军烟草新材料科技有限公司 | Pickering emulsifier and preparation method and application thereof |
| CN115417903A (en) * | 2022-08-30 | 2022-12-02 | 陕西德冠生物科技有限公司 | A kind of industrial purification method of rhamnolipid |
| WO2026008334A1 (en) * | 2024-07-02 | 2026-01-08 | Evonik Operations Gmbh | Spray drying process of biosurfactants |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0550448B1 (en) * | 1990-09-25 | 1994-05-25 | Südzucker Aktiengesellschaft Mannheim/Ochsenfurt | Process for producing rhamnose from rhamnolipids |
| CN1891831A (en) * | 2005-07-01 | 2007-01-10 | 南京理工大学 | Method for preparing rhamnolipid |
| CN1974589A (en) * | 2006-12-15 | 2007-06-06 | 湖南大学 | Process of extracting rhanolipid as biosurfactant |
| CN101613381A (en) * | 2009-08-05 | 2009-12-30 | 河北鑫合生物化工有限公司 | The method for post extraction of rhamnolipid |
| CN101787057A (en) * | 2010-02-09 | 2010-07-28 | 华东理工大学 | Method for separating and purifying rhamnolipid |
| CN101845468A (en) * | 2010-03-30 | 2010-09-29 | 湖州紫金生物科技有限公司 | Preparation method and application of rhamnolipid |
-
2011
- 2011-09-19 CN CN2011102774253A patent/CN102766172A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0550448B1 (en) * | 1990-09-25 | 1994-05-25 | Südzucker Aktiengesellschaft Mannheim/Ochsenfurt | Process for producing rhamnose from rhamnolipids |
| CN1891831A (en) * | 2005-07-01 | 2007-01-10 | 南京理工大学 | Method for preparing rhamnolipid |
| CN1974589A (en) * | 2006-12-15 | 2007-06-06 | 湖南大学 | Process of extracting rhanolipid as biosurfactant |
| CN101613381A (en) * | 2009-08-05 | 2009-12-30 | 河北鑫合生物化工有限公司 | The method for post extraction of rhamnolipid |
| CN101787057A (en) * | 2010-02-09 | 2010-07-28 | 华东理工大学 | Method for separating and purifying rhamnolipid |
| CN101845468A (en) * | 2010-03-30 | 2010-09-29 | 湖州紫金生物科技有限公司 | Preparation method and application of rhamnolipid |
Non-Patent Citations (2)
| Title |
|---|
| 《粮食与食品工业》 20051231 刘晓庚，等 "微胶囊制备方法的比较" 第28-31页 1 第12卷, 第1期 * |
| 刘晓庚，等: ""微胶囊制备方法的比较"", 《粮食与食品工业》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016115048A1 (en) | 2015-01-12 | 2016-07-21 | Logos Technologies, Llc | Production of rhamnolipid compositions |
| US9884883B2 (en) | 2015-01-12 | 2018-02-06 | Logos Technologies, Llc | Production of rhamnolipid compositions |
| US10829507B2 (en) | 2017-02-06 | 2020-11-10 | Stepan Company | Decolorization of concentrated rhamnolipid composition |
| CN107937273A (en) * | 2017-11-22 | 2018-04-20 | 北京沃太斯环保科技发展有限公司 | Rhamnolipid is used as protectant application in microniological proudcts spray-drying process |
| CN111205844A (en) * | 2020-03-09 | 2020-05-29 | 陕西斯普曼生物工程有限公司 | Treatment method of rhamnolipid fermentation liquor of oil field oil displacement agent |
| CN111205844B (en) * | 2020-03-09 | 2022-03-08 | 陕西斯普曼生物工程有限公司 | Treatment method of rhamnolipid fermentation liquor of oil field oil displacement agent |
| CN112108075A (en) * | 2020-08-19 | 2020-12-22 | 山东将军烟草新材料科技有限公司 | Pickering emulsifier and preparation method and application thereof |
| CN115417903A (en) * | 2022-08-30 | 2022-12-02 | 陕西德冠生物科技有限公司 | A kind of industrial purification method of rhamnolipid |
| WO2026008334A1 (en) * | 2024-07-02 | 2026-01-08 | Evonik Operations Gmbh | Spray drying process of biosurfactants |
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Application publication date: 20121107 |