CN102754696B - Winterworm summerherb strain and winterworm summerherb walnut peptide health drink prepared from same - Google Patents
Winterworm summerherb strain and winterworm summerherb walnut peptide health drink prepared from same Download PDFInfo
- Publication number
- CN102754696B CN102754696B CN2012102128814A CN201210212881A CN102754696B CN 102754696 B CN102754696 B CN 102754696B CN 2012102128814 A CN2012102128814 A CN 2012102128814A CN 201210212881 A CN201210212881 A CN 201210212881A CN 102754696 B CN102754696 B CN 102754696B
- Authority
- CN
- China
- Prior art keywords
- walnut
- winterworm
- summerherb
- enzymolysis
- cordyceps
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000009496 Juglans regia Nutrition 0.000 title claims abstract description 56
- 235000020234 walnut Nutrition 0.000 title claims abstract description 56
- 230000036541 health Effects 0.000 title claims abstract description 17
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 17
- 240000007049 Juglans regia Species 0.000 title 1
- 241000758789 Juglans Species 0.000 claims abstract description 55
- 238000000034 method Methods 0.000 claims abstract description 17
- 241000233866 Fungi Species 0.000 claims abstract description 15
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 11
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 9
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 9
- 235000015097 nutrients Nutrition 0.000 claims abstract description 6
- 241000190633 Cordyceps Species 0.000 claims description 23
- 239000007788 liquid Substances 0.000 claims description 20
- 241001248610 Ophiocordyceps sinensis Species 0.000 claims description 15
- 240000001307 Myosotis scorpioides Species 0.000 claims description 12
- 235000013361 beverage Nutrition 0.000 claims description 11
- 229930006000 Sucrose Natural products 0.000 claims description 10
- 230000001737 promoting effect Effects 0.000 claims description 10
- 239000005720 sucrose Substances 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 9
- -1 sucrose ester Chemical class 0.000 claims description 7
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 claims description 5
- 239000004278 EU approved seasoning Substances 0.000 claims description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- 235000011194 food seasoning agent Nutrition 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 239000002002 slurry Substances 0.000 claims description 4
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims description 3
- 238000010009 beating Methods 0.000 claims description 3
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims description 3
- 238000012859 sterile filling Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 239000000811 xylitol Substances 0.000 claims description 3
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims description 3
- 229960002675 xylitol Drugs 0.000 claims description 3
- 235000010447 xylitol Nutrition 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 2
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 abstract description 6
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 abstract description 6
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 abstract description 6
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 4
- 239000002994 raw material Substances 0.000 abstract description 2
- 150000003384 small molecules Chemical class 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 239000002131 composite material Substances 0.000 abstract 1
- 230000000593 degrading effect Effects 0.000 abstract 1
- 230000000144 pharmacologic effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 7
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 239000000796 flavoring agent Substances 0.000 description 4
- 235000019634 flavors Nutrition 0.000 description 4
- 210000004072 lung Anatomy 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 3
- 229960005305 adenosine Drugs 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 235000015961 tonic Nutrition 0.000 description 3
- 230000001256 tonic effect Effects 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 206010062717 Increased upper airway secretion Diseases 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
- 230000003712 anti-aging effect Effects 0.000 description 2
- 235000012000 cholesterol Nutrition 0.000 description 2
- 229910052804 chromium Inorganic materials 0.000 description 2
- 239000011651 chromium Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 208000026435 phlegm Diseases 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 229960000716 tonics Drugs 0.000 description 2
- 229960003487 xylose Drugs 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- 208000004998 Abdominal Pain Diseases 0.000 description 1
- 244000144730 Amygdalus persica Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000255789 Bombyx mori Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 208000002881 Colic Diseases 0.000 description 1
- 206010010144 Completed suicide Diseases 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 206010044302 Tracheitis Diseases 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 235000019498 Walnut oil Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 150000003838 adenosines Chemical class 0.000 description 1
- 238000010564 aerobic fermentation Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000000954 anitussive effect Effects 0.000 description 1
- 230000001088 anti-asthma Effects 0.000 description 1
- 229940124584 antitussives Drugs 0.000 description 1
- 210000003056 antler Anatomy 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000009084 cardiovascular function Effects 0.000 description 1
- 208000023819 chronic asthma Diseases 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 210000003792 cranial nerve Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- ZZVUWRFHKOJYTH-UHFFFAOYSA-N diphenhydramine Chemical compound C=1C=CC=CC=1C(OCCN(C)C)C1=CC=CC=C1 ZZVUWRFHKOJYTH-UHFFFAOYSA-N 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 208000014617 hemorrhoid Diseases 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 235000013666 improved nutrition Nutrition 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 230000009916 joint effect Effects 0.000 description 1
- 235000014058 juice drink Nutrition 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 230000013777 protein digestion Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 230000002040 relaxant effect Effects 0.000 description 1
- 150000004671 saturated fatty acids Chemical class 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 229940126680 traditional chinese medicines Drugs 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000008170 walnut oil Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The invention discloses a winterworm summerherb strain and a method for preparing a winterworm summerherb walnut peptide health drink by using the winterworm summerherb strain. The method comprises the steps of: with walnut juice as a raw material, fermenting the winterworm summerherb fungus under a proper condition, and degrading protein in the winterworm summerherb fungus by adopting a composite enzyme system so that most of the winterworm summerherb fungus is degraded into small molecule peptides; and finally, preparing the health drink with proper mouth feel. According to the winterworm summerherb walnut peptide health drink, the basic nutrients of the walnut are kept, and cordycepin (8mg/mL) and the small molecule peptides are increased. The drink prepared by adopting the method ensures that the nutrients and the pharmacologic action of the walnut and the winterworm summerherb are organically combined so that the health function is further improved; and the method has the advantages of reasonable steps, simple equipment and strong maneuverability.
Description
Technical field
The invention belongs to microorganism and food technology field, relate to the preparation of health promoting beverage, particularly a kind of Cordyceps strain and adopt the Chinese caterpillar fungus walnut peptide health promoting beverage of this Cordyceps strain preparation.
Background technology
The pharmaceutical use of walnut is very high, and the traditional Chinese medical science is widely used.Motherland's medical science thinks that walnut is warm in nature, flavor is sweet, nontoxic, stomach invigorating is arranged, enriches blood, moistening lung, effect such as repose.Shennong's Herbal with walnut classify as clothes of a specified duration make light of one's life by commiting suicide beneficial gas, promote longevity top grade.Record and narrate in the numerous work in Meng the Tang Dynasty " Shiliao Bencao ", eating walnut kernel can whet the appetite, and logical profit blood vessels make the kindred exquisiteness.Record and narrate in the works such as Song dynasty Liu Han " Kaibao Bencao ", and walnut kernel " order of food is fertile strong, profit flesh, black beard and hair is eaten sharp little water more, goes five hemorrhoid ".Ming Dynasty's LI Shi-Zhen work Compendium of Material Medica is recorded and narrated, and walnut kernel has effects such as " the beneficial gate of vitality locate three warmers for benefiting qi and nourishing blood, moistening the lung and resolving the phlegm, and warm lung ease constipation is controlled the cold of insufficiency type and breathed with cough, and the waist pin is heavily painful, trusted subordinate's colic, bloody flux intestines wind ".
Modern medicine study thinks that the phosphatide in the walnut has excellent health functions to cranial nerve.Walnut oil contains unsaturated fatty acids, and the arteriosclerotic effect of control is arranged.Contain the indispensable trace elements of human body such as zinc, manganese, chromium in the walnut kernel.Human body zinc, manganese content in aging course day by day reduce, and chromium has glucose utilization, cholesterol metabolic and the cardiovascular function of protection of promotion.The antitussive and antiasthmatic effect of walnut kernel is also fairly obvious, and is winter, splendid to chronic tracheitis and asthma patient curative effect.As seen frequent edible walnut can be healthy and strong, brain tonic, again can be anti-ageing.Contained vitamin E in the walnut kernel can make cell avoid the oxidative damage of free radical, is the anti-ageing material that medical circle is generally acknowledged, so walnut has the title of " long live ", " long-lived fruit ".Walnut has multiple unsaturated and single non-saturated fatty acid, can reducing cholesterol content.Therefore eat the nuclear walnut human heart is had certain benefit.No matter be the usefulness of making up a prescription, still eat something rare separately, poach, dip in, cook as sugar, enrich blood foster the spirit of nobility, good effects such as kidney tonifying, essence replenishing, relieving cough and asthma, moistening dryness for relaxing bowels.
Cordyceps sinensis (Cordyceps Sinensis), also claim Chinese Cordyceps sinensis, be that Chinese Chinese caterpillar fungus is contaminated Chinese caterpillar fungus bat moth larvae and the worm that forms, careless combination, mainly be distributed in the high and cold grassy marshland of the sea level elevation 3500m ~ 5200m such as Sichuan, Yunnan, Qinghai, Tibet, Gansu and Guizhou of China, its artificial culture is difficulty comparatively.Describe in the Compendium of Material Medica " winter in soil, shape such as the winter silkworm, grow on the soil to the summer pool, connect body and turn to grass ", people are referred to as Cordyceps sinensis with its form.The Cordyceps sinensis living environment is special, poor growth, seasonal strong, yield poorly, expensive like gold, the saying that medical circle spreads to be had " one of peaceful Chinese caterpillar fungus, the not full car of gold and jade ".
Since ancient times, Cordyceps sinensis is the famous and precious wild Chinese medicinal materials of China, have tonifying deficiency, beneficial vital essence, protect effects such as lung, kidney-nourishing, hemostasis and phlegm, strengthening by means of tonics, have and exceed medicinal history in thousand, and there is the ancient medical book that surpasses 70 ones to record, it is praised highly is " traditional Chinese medicines in thousand ", " king in the medicine ", is listed in China's three big tonics with genseng, pilose antler.
Wild cordyceps is the exclusive resource of China, is listed in national second class protection species, and Cordyceps sinensis contains rich in protein, VITAMIN and mineral element.Its main medicinal ingredients is cordycepin, cordycepic acid, Cordyceps polysaccharide, adenosine, SOD etc.The wild cordyceps resource is very deficient, and the area that has has been on the verge of disappearance, thereby the disparities between supply and demand in market are very sharp-pointed.
Summary of the invention
The objective of the invention is to, a strain Cordyceps fungus is provided and adopts this Cordyceps strain to prepare the method for Chinese caterpillar fungus walnut peptide health promoting beverage.
In order to realize above-mentioned task, the present invention takes following technical solution:
One strain Cordyceps fungus is characterized in that, this Cordyceps fungus called after CS-18, and preserving number is CGMCC No.6006.
Adopt above-mentioned Cordyceps strain to prepare the method for Chinese caterpillar fungus walnut peptide health promoting beverage, it is characterized in that, follow these steps to operation:
1) preparation of bacterial classification: under aseptic condition, the Cordyceps strain CS-18 that activates is transferred on liquid PDA substratum, cultivated 5～15 days in 15 ℃～30 ℃ conditions, for the preparation of the secondary kind; The secondary kind also adopts the PDA liquid nutrient medium, with the secondary kind again expanding propagation produce three grades of kinds;
2) walnut juice fermentation: with making beating after the walnut kernel peeling, add soluble sugar, the add-on of soluble sugar is walnut kernel quality 5% ~ 10%, sterilization, inoculation Cordyceps strain, 20 ℃ ~ 28 ℃ of leavening temperatures, every 5h, 100rpm stirs 20-30min, ferments with this understanding 5 ~ 12 days;
3) enzymolysis: after the fermentation ends, press 0.1%-0.5% of walnut kernel quality and add papoid, keep 55 ℃ ~ 65 ℃ of temperature, degradation time 3-8 hour;
4) allotment: add an amount of seasonings (edible sucrose 4%-5%, wood sugar ester 0.01-0.02%, sucrose ester 0.1%-0.2%, Tegin 55G 0.1%, protein sugar 0.05%-0.1%), stir;
5) method is routinely carried out homogeneous, Ultra High Temperature Short Time, sterile filling, is labelled, and has both got Chinese caterpillar fungus walnut peptide health care finished product.
The present invention inoculates Cordyceps strain CS-18 in walnut juice, stimulate it to produce the generation of materials such as cordycepin and adenosine, and the macromolecular substance such as protein in the degraded walnut juice, make remaining albumen further be converted into polypeptide by enzymolysis then, improve the nutrition of walnut, give its nourishing function.
Compare with existing walnut class beverage, Chinese caterpillar fungus walnut peptide health promoting beverage of the present invention has advantage and technique effect is as follows:
(1) strain excellent of Cordyceps sinensis is used for the production of walnut drink, has increased the zymotechnique link.
(2) walnut juice fermented product is adopted the degraded of prozyme system and degradation technique thereof, make its most of protein transduction turn to polypeptide.Be conducive to the absorption of this nutritive ingredient.
(3) given walnut juice drink special composition, i.e. cordycepin, Cordyceps polysaccharide, cordycepic acid, adenosine and SOD etc. have greatly improved nutrition and the nourishing function of walnut juice.
(4) have special local flavor, not only keep the original local flavor of walnut juice, also give its special fragrance, have good market outlook.
(5) processing step is reasonable, equipment is simple, and is workable.
Description of drawings
Fig. 1 is process flow sheet of the present invention.
The present invention is described in further detail below in conjunction with drawings and Examples.
Embodiment
The applicant is in research process; filter out a strain and be suitable for the Cordyceps sinensis bacteria strain that walnut juice ferments; in order to protect this bacterial strain; the applicant is except school preserves; and be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on April 18th, 2012; the depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica (postcode: 100101), the classification called after of suggestion: Cordyceps sinensis.This Cordyceps fungus called after CS-18, preserving number is CGMCC No.6006.
In order to take full advantage of walnut and Cordyceps sinensis effective constituent separately, and strengthen its joint effect, the applicant is raw material with walnut juice, adopt growth fast, the Cordyceps strain CS-18 that the cordycepin productive rate is high through special zymotechnique and enzymolysis process, produces and both contains active ingredients of cordyceps sinensis, and when keeping the various nutritive substances of walnut, making protein digestion is the health promoting beverage that contains polypeptide.
Below be the embodiment that the contriver provides, need to prove, the invention is not restricted to this embodiment.
1) preparation of bacterial classification: under aseptic condition, the Cordyceps strain CS-18 that activates is transferred on substratum, in 15～30 ℃ of cultivations 5～15 days, for the preparation of the secondary kind; The secondary kind adopts the PDA liquid nutrient medium, with the secondary kind again expanding propagation produce three grades of kinds;
2) get clean high-quality walnut kernel 50kg peeling, making beating obtains walnut kernel slurries 1000L; Add soluble sugar 10kg at the peach kernel slurries, sterilization, cooling inoculate Cordyceps sinensis liquid spawn (50L); 10 ℃～35 ℃ of leavening temperatures, aerobic fermentation, fermentation time kept 5～12 days, and in the fermenting process, every 5h, 100rpm stirs 20min-30min;
3) enzymolysis: after the fermentation ends, in fermented liquid, press 0.1%～0.5% of walnut kernel quality and add papoid, keep 55 ℃～65 ℃ of temperature, degradation time 3 hours～8 hours;
4) allotment: add seasonings in the liquid behind enzymolysis, in the present embodiment, seasonings is edible sucrose, Xylitol, sucrose ester, Tegin 55G and protein sugar.Wherein, the edible sucrose consumption be behind the enzymolysis liquid mass 4% ~ 5%, wood sugar ester consumption is the 0.01-0.02% of the liquid mass behind the enzymolysis, the sucrose ester consumption is the liquid mass 0.1% ~ 0.2% behind the enzymolysis, the Tegin 55G consumption is the liquid mass 0.08%-0.12% behind the enzymolysis, the protein sugar consumption is the liquid mass 0.05%-0.1% behind the enzymolysis, stirs.
5) method is routinely carried out homogeneous, Ultra High Temperature Short Time; Then sterile filling, label, get product.
After measured, cordycepin content reaches more than the 8mg/mL in the Chinese caterpillar fungus walnut peptide health promoting beverage for preparing, and adenosine class material increases substantially, and not only keeps the original local flavor of walnut juice, also gives its special fragrance, has good market outlook.
Claims (3)
1. a Cordyceps fungus is characterized in that, this Cordyceps fungus classification called after Cordyceps sinensis (Cordyceps Sinensis) CS-18, and preserving number is CGMCC No.6006.
2. adopt the described Cordyceps fungus of claim 1 to prepare the method for Chinese caterpillar fungus walnut peptide health promoting beverage, it is characterized in that, follow these steps to operation:
1) preparation of bacterial classification: under aseptic condition, the Cordyceps strain CS-18 that activates is transferred on the PDA liquid nutrient medium, in 15～30 ℃ of cultivations 5～15 days, for the preparation of the secondary kind; The secondary kind also adopts the PDA liquid nutrient medium, with the secondary kind again expanding propagation produce three grades of kinds;
2) walnut juice fermentation: with making beating after the walnut kernel peeling, obtain the walnut kernel slurries, in the walnut kernel slurries, add soluble sugar, the add-on of soluble sugar is 5% ~ 10% of walnut kernel quality, sterilization, inoculation Cordyceps strain, 15 ℃ ~ 28 ℃ of leavening temperatures, every 5h, 100rpm stirs 20-30min, ferments with this understanding 5 ~ 12 days;
3) enzymolysis: after the fermentation ends, in fermented liquid, press 0.1%-0.5% of walnut kernel quality and add papoid, keep 55 ℃ ~ 65 ℃ of temperature, enzymolysis time 3-8 hour;
4) allotment: add seasonings in the liquid behind enzymolysis, stir;
5) method is routinely carried out homogeneous, Ultra High Temperature Short Time, sterile filling, labels, and gets Chinese caterpillar fungus walnut peptide health promoting beverage.
3. method as claimed in claim 2, it is characterized in that, described seasonings is edible sucrose, Xylitol, sucrose ester, Tegin 55G and protein sugar, wherein, the edible sucrose consumption be behind the enzymolysis liquid mass 4% ~ 5%, the Xylitol consumption is the 0.01-0.02% of the liquid mass behind the enzymolysis, the sucrose ester consumption is the liquid mass 0.1% ~ 0.2% behind the enzymolysis, the Tegin 55G consumption is the liquid mass 0.08%-0.12% behind the enzymolysis, and the protein sugar consumption is the liquid mass 0.05%-0.1% behind the enzymolysis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102128814A CN102754696B (en) | 2012-06-22 | 2012-06-22 | Winterworm summerherb strain and winterworm summerherb walnut peptide health drink prepared from same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102128814A CN102754696B (en) | 2012-06-22 | 2012-06-22 | Winterworm summerherb strain and winterworm summerherb walnut peptide health drink prepared from same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102754696A CN102754696A (en) | 2012-10-31 |
| CN102754696B true CN102754696B (en) | 2013-08-07 |
Family
ID=47049543
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012102128814A Active CN102754696B (en) | 2012-06-22 | 2012-06-22 | Winterworm summerherb strain and winterworm summerherb walnut peptide health drink prepared from same |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102754696B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102894349B (en) * | 2012-10-17 | 2013-11-06 | 西北农林科技大学 | Method for using Chinese caterpillar fungus strain to produce Chinese caterpillar fungus fermented meat |
| CN102907502A (en) * | 2012-11-05 | 2013-02-06 | 贵州神奇集团控股有限公司 | Cordyceps sinensis health beverage and preparation method thereof |
| CN103255071B (en) * | 2013-04-28 | 2014-10-08 | 西北农林科技大学 | Yeast strain and method for brewing cordyceps sinensis wine by using same |
| CN103478784B (en) * | 2013-09-09 | 2015-04-08 | 江苏丘陵地区镇江农业科学研究所 | Method for preparing walnut kernel polypeptide through bacillus natto fermentation |
| CN103462022B (en) * | 2013-09-24 | 2014-12-31 | 郑剑峰 | Cordyceps militaris nourishing paste and preparation method thereof |
| CN108200965B (en) * | 2018-01-24 | 2021-11-05 | 化泽瑜 | Method for preparing compound cordycepin soybean milk |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20050012203A (en) * | 2004-12-18 | 2005-01-31 | 윤영숙 | The manufacturing method of health drink for prevention and treatment of asthma |
| CN101077209B (en) * | 2006-05-22 | 2010-05-12 | 陕西天宝大豆食品技术研究所 | Walnut lactic acid bacteria beverage and preparing process thereof |
| CN1943370A (en) * | 2006-11-01 | 2007-04-11 | 曾树生 | Method for producing caterpillar fungus milk |
| CN102389145B (en) * | 2011-11-12 | 2013-06-12 | 陈宏伟 | Method for preparing composite beverage by fermenting Cordyceps militaris |
-
2012
- 2012-06-22 CN CN2012102128814A patent/CN102754696B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN102754696A (en) | 2012-10-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102754696B (en) | Winterworm summerherb strain and winterworm summerherb walnut peptide health drink prepared from same | |
| CN101543259B (en) | Preparation method of biological feed additive | |
| CN102604804B (en) | A kind of cordyceps wine and preparation method thereof | |
| CN103385518B (en) | A kind of seabuckthorn snow chrysanthemum fruit vinegar drink and preparation method thereof | |
| CN104957623A (en) | Preparation method of ginseng composite fruit-vegetable enzyme and a product | |
| CN104256584A (en) | Moringa oleifera ferment and preparation method thereof | |
| CN103392827B (en) | Moringa leaf preserved bean curd | |
| CN106754142A (en) | A kind of deer-blood wine and preparation method thereof | |
| CN104232441A (en) | Health caring and preserving cordyceps wine and preparation method thereof | |
| CN103169126A (en) | Lucid ganoderma, worm grass and red jujube slurry | |
| CN104856026A (en) | Preparation method of cordyceps and arctium lappa enzyme | |
| CN101803769A (en) | Soup base and preparation process thereof | |
| CN103525636A (en) | Formula of ganoderma lucidum wine and preparation method of ganoderma lucidum wine | |
| CN102885349A (en) | Glossy ganoderma and red date thick slurry | |
| CN101613652A (en) | Tuckahoe health care white spirit and preparation method thereof | |
| CN102885352A (en) | Thick slurry of mushrooms and red dates | |
| CN102586048B (en) | Health care alcohol brewed with cordyceps sinensis funginite | |
| CN102488213B (en) | Bioorganic nutrient solution | |
| CN108991498A (en) | Ferment and preparation method thereof | |
| CN101401646A (en) | Soup additive | |
| CN104531453B (en) | A kind of production method of Pedicellus et Pericarpium Trapae wine | |
| CN106071587A (en) | With Herba Dendrobii juice for a kind of Cordyceps biological drink of carrier preparation | |
| CN105900695B (en) | Cordyceps mycelia is prepared by culture medium of dendrobium candidum | |
| CN103589569B (en) | Production technique of compound fermented ulva health wine | |
| CN102894428A (en) | Dense schizophyllum commune red date milk |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |