CN102749100A - Biological behavior sensor and monitoring method based on quadrupole impedance and three-dimensional tracing - Google Patents
Biological behavior sensor and monitoring method based on quadrupole impedance and three-dimensional tracing Download PDFInfo
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- CN102749100A CN102749100A CN2012102152599A CN201210215259A CN102749100A CN 102749100 A CN102749100 A CN 102749100A CN 2012102152599 A CN2012102152599 A CN 2012102152599A CN 201210215259 A CN201210215259 A CN 201210215259A CN 102749100 A CN102749100 A CN 102749100A
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Abstract
The invention discloses a biological behavior sensor and a monitoring method based on quadrupole impedance and three-dimensional tracing. The biological behavior sensor is characterized in that the biological behavior sensor comprises a running water system, a quadrupole impedance behavior acquisition system, a three-dimensional tracing behavior acquisition and a data processing center. Biological behavior signals are acquired through a quadrupole impedance behavior acquisition technique and a three-dimensional tracing behavior acquisition technique, and the real-time analysis and unification of two kinds of behavior signals are realized through compatibility processing. Not only can the relationship between biological behavior strength and the environment be analyzed on line, but also the relationship between different behavior modes and an environment can be researched through tracing analysis, and finally the online monitoring and analysis between biological behavior influence and environmental change within a certain environment can be realized.
Description
Technical Field
The invention belongs to the technical field of environmental monitoring, and relates to a biological sensor for monitoring biological behavior change, in particular to a biological behavior sensor and a monitoring method based on quadrupole impedance and three-dimensional tracing.
Background
The internal steady state (Homeostasis) mechanism of the organism can regulate and control the internal environment of the organism per se, keep the organism relatively stable and reduce the dependence on external environmental conditions. Organisms have developed many complex morphological and physiological adaptations in order to maintain homeostasis, the simplest of which, the most common, being adaptation by means of behaviour, for example animals avoiding adverse environmental conditions by means of behaviour.
The change of biological behavior has a certain relation with the surrounding environment. The organism can control the internal environment to a proper level to a great extent through the regulation of a behavior mechanism, thereby greatly increasing the time and space of the biological activity and reducing the dependence on the external environment. Therefore, with the change of certain influence factors or the increase of influence in the environment, the aquatic animals can maintain the in vivo environment stable in a short time by regulating through self behavior mechanisms, gradually adapt to the environment and avoid acute damage to the aquatic animals caused by polluted water environment to a great extent.
At present, there are biosensors for collecting behavior signals in a manner of electric field variation and analyzing them on line for behavior variation of aquatic organisms, such as fish and aquatic cladocera. The sensor comprises a biological behavior signal acquisition sensor such as an electric field or a magnetic field (E/M Fields Biosensor), an Image pickup (Digital Image Recording System) and the like. The electric field or magnetic field biological behavior sensor can only analyze biological behavior signals by the interference degree of magnetic field or electric field signals in a certain range, and is difficult to realize signal acquisition and analysis such as avoidance behavior, swimming speed, C-shaped motion and the like. Although the imaging sensor can determine the living state of a living being by recording the position of the living being on line, it is difficult to quantify the relationship between the behavior change of the living being and the environmental stress.
Therefore, in order to realize online monitoring and analysis of behavior changes of aquatic organisms in a certain environment, including a normal environment and a polluted environment, a brand-new biosensor must be constructed, not only can the relationship between behavior intensity and the environment be analyzed online, but also the relationship between different behavior modes and the environment can be researched through tracing analysis, and finally the relationship analysis between biological behavior response and the environment changes is realized.
Disclosure of Invention
The invention aims to provide an aquatic behavior sensor based on quadrupole impedance and three-dimensional tracing technology aiming at the requirement of a biological behavior sensor for analyzing the relation between biological behavior response and environmental change, solves the problems of the existing biological behavior sensors and realizes the online signal acquisition and analysis of the behavior change of aquatic organisms.
The purpose of the invention is realized by the following technical scheme:
a biological behavior sensor based on quadrupole impedance and three-dimensional tracing is characterized by comprising a flow system, a quadrupole impedance behavior acquisition system, a three-dimensional tracing behavior acquisition system and a data processing center, wherein,
the water flowing system comprises a biological behavior monitoring area, wherein a bottom cover and a top cover are respectively arranged at two ends of the biological behavior monitoring area, a water inlet is formed in the bottom cover, a sensor opening is formed in the top cover, a sensor overflow port is formed in the side face of the sensor opening, and silica gel pads with holes are respectively arranged between the biological behavior monitoring area and the bottom cover and between the biological behavior monitoring area and the top cover;
the quadrupole impedance behavior acquisition system comprises an electrode, the electrode is arranged in a biological behavior monitoring area, an electrode contact is arranged on the electrode, and the electrode contact is connected with special signal identification software through a signal wire;
the three-dimensional tracing behavior acquisition system comprises a camera, wherein the camera is arranged in a biological behavior monitoring area, and is connected with a video signal line which is connected with video signal analysis software;
virtual signals collected in the quadrupole impedance behavior collection system and the three-dimensional tracing behavior collection system are respectively converted into digital signals and transmitted to a data processing center.
A monitoring method using the biological behavior sensor comprises the following steps:
a) culturing and selecting the indicator organism;
b) placing an indicator organism into the flow system;
c) a quadrupole impedance behavior acquisition system is used for acquiring signals and transmitting the signals to special signal identification software for analysis and processing;
collecting signals by using a three-dimensional tracing behavior collection system, transmitting the signals to video signal analysis software, and analyzing and processing the signals;
d) and c, transmitting the processing signal in the step c to a data processing center for analysis and judgment.
The invention constructs a set of brand-new biosensor system, which collects biological behavior signals through a quadrupole impedance behavior collection technology and a three-dimensional tracing behavior collection technology, and realizes real-time analysis and unification of the two behavior signals through compatibility processing, thereby not only being capable of analyzing the relationship between the biological behavior intensity and the environment on line, but also being capable of researching the relationship between different behavior modes and the environment through tracing analysis, and finally realizing on-line monitoring and analysis between biological behavior response and environmental change in a certain environment including normal environment and polluted environment. Compared with the prior art, the invention has the characteristics of remarkable progress and substantive feature.
The present invention will be explained in detail below with reference to the drawings and examples.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 shows behavior changes of medaka in Japan collected by the quadrupole impedance behavior collection system of the biological behavior sensor according to the present invention; in the figure, the abscissa represents time, and the ordinate represents the change in the behavior intensity of a Japanese mackerel medaka;
FIG. 3 shows behavior changes of a Japanese medaka collected by the three-dimensional tracing behavior collection system of the biological behavior sensor according to the present invention;
FIG. 4 is a biological behavior model based on the results of comprehensive analysis of behavior changes of Japanese medaka.
Detailed Description
With reference to figure 1 of the drawings,
the biosensor comprises a flow system, a quadrupole impedance behavior acquisition system, a three-dimensional tracing behavior acquisition system and a data processing center. Wherein,
the flowing water system comprises a biological behavior monitoring area 2, the biological behavior monitoring area 2 can be manufactured into a transparent cylindrical pipe, two ends of the transparent cylindrical pipe are respectively provided with a bottom cover 3 and a top cover 4, the bottom cover 3 is provided with a water inlet 1, the top cover 4 is provided with a sensor opening 5, the side face of the sensor opening 5 is provided with a sensor overflow port 6, a porous silica gel pad 9 and a porous silica gel pad 10 are respectively padded between the transparent cylindrical pipe and the bottom cover 3 and between the transparent cylindrical pipe and the top cover 4, and the aperture of the silica gel pad is determined by a tested organism.
The quadrupole impedance behavior acquisition system mainly comprises 2 groups of stainless steel electrodes 11, the electrodes 11 are arranged on the inner wall of a transparent cylindrical tube, each group of electrodes consists of two pairs of electrodes which are oppositely arranged, one pair of the electrodes is a transmitting signal electrode, the other pair of the electrodes is a receiving signal electrode, the structure of each group of electrodes is consistent, a plurality of electrode contacts 7 are arranged on the electrodes 11, and the electrode contacts 7 are connected with special signal recognition software 18 through signal wires 12.
The three-dimensional tracing behavior acquisition system is mainly based on a binocular vision technology, two parallel cameras 8 are arranged on a transparent cylindrical tube, and the cameras 8 are connected with video signal analysis software 17 through video signal lines 13.
The virtual signals collected in the quadrupole impedance behavior collection system and the three-dimensional tracing behavior collection system are converted into digital signals 19 respectively and transmitted to a data processing center 20 (computer).
In the above technical solution, the water inlet 1 is connected with an external water pipe, and a flow meter can be installed on a water inlet pipe of the external water pipe, so as to control water inlet and outlet through the flow meter.
In the above technical solution, the pipe of the biological behavior monitoring area 2 may be selected according to the property of the monitored water body, for example, the pipe may be made of glass material, PVC material, or ABS material.
In the above technical solution, the electrodes are made of stainless steel material, and the size of the electrodes is determined according to the size of the tested organism and the size of the biological behavior monitoring area 2.
In the technical scheme, the overflow port 6 of the sensor is connected with the drain pipe, so that the monitored water body is led out.
Brief description of the working principle
In the quadrupole impedance behavior acquisition system of the sensor, the transmitting signal electrode continuously transmits low-voltage sine high-frequency signals, a low-voltage sine high-frequency signal electric field is formed in the sensor, when the electric field changes due to the movement of a tested organism in the sensor, the receiving signal electrode can sense the change of the electric field, the electric signal change formed by the transmitting signal electrode in the receiving sensor is transmitted to the special signal identification software 18 through different electrode contacts 7 by the signal wire 12, the biological behavior is analyzed, the biological behavior signal acquisition is realized, the acquired virtual signal is converted into a digital signal 19 and is transmitted to the biological behavior data processing center 20. The signal transmitting electrode and the signal receiving electrode are determined by the signal 12.
In the three-dimensional tracing behavior acquisition system of the sensor, the two parallel cameras 8 based on the binocular vision technology finally form a three-dimensional monitoring technology to trace biological behaviors in a certain area. After the video signals are collected on line by the two parallel cameras 8, the video signals are transmitted to the video signal analysis software 17 through the video signal line 13 for analysis. In the video signal analysis software 17, the background value of the background signal 14 is filtered out to form the signal source 15 of only the tested organism, and the tracing analysis 16 is used to trace the biological behavior. Meanwhile, the video signal analysis software 17 converts the acquired virtual signal into a digital signal 19, and transmits the digital signal to the data processing center 20.
After the biological behavior signal acquisition and the three-dimensional tracing behavior acquisition based on the quadrupole impedance, the behavior digital signal is synchronously analyzed by the data processing center 20, so that the online analysis of the biological behavior change is realized.
The monitoring area of the biological behavior sensor can be selected according to the size of an individual tested organism, and the number of the tested organisms in the biological behavior sensor is kept to be 5-10.
The tested organisms loaded in each biological behavior sensor are aquatic organism larvae of a monitored water source protospecies 24-72 hours (preferably 48 hours) after birth, and conventional standard water culture evolution is adopted in a laboratory for at least more than three generations to serve as the tested organisms; or the aquatic organism larvae of the monitored water source standard mode organisms 24-72 hours (preferably 48 hours) after birth are directly used as the tested organisms;
the water source primitive species to be monitored comprises aquatic invertebrates and vertebrates, wherein the invertebrates are mainly selected from arthropods such as shrimps, fleas and the like, and the vertebrates are mainly selected from fishes with the body length of about 4 cm;
the standard model creatures of the monitored water source comprise invertebrate macrobrachium nipponensis, macrophylla, vertebrate Japanese medaka, gobiocypris rarus, zebrafish and the like.
The invention provides a biological behavior sensor for monitoring behavior change of aquatic organisms, which preselects aquatic organisms with motion behavior diversity as an indicating species, and evaluates biological behavior by monitoring at one point or multiple points and motion behavior change of a tested organism at different positions in one or more water bodies.
Examples of the experiments
The following will specifically describe the monitoring of biological behavior of a Japanese medaka as an example.
Referring to fig. 1, a biological behavior sensor for monitoring medaka in japan was fabricated, the biological behavior sensor having a monitoring area 2 fabricated of plexiglass, the monitoring area 2 having a size of inner diameter of phi 4cm x outer diameter of 5 cm. 2 pairs of electrodes made of stainless steel sheets are arranged on the inner wall of the monitoring area 2, the electrodes are selected to be 2.4 multiplied by 5cm, and the two pairs of electrodes are connected with a signal processing center through stainless steel screws, wherein one pair of electrodes is a signal transmitting electrode, and the other pair of electrodes is a signal receiving electrode; the two ends of the monitoring area 2 are respectively fixed with a round cover, the center of the round cover at the bottom is connected with a water inlet 1 and is connected with an external water supply pipe, the center of the upper cover is an open overflow port 6, and the round cover and the monitoring area 2 are sealed by a silica gel pad with holes which are adaptive to the tested organisms. And (3) inlaying a pair of cameras 8 capable of binocular vision at appropriate positions of a top cover of the biological behavior monitoring area, and carrying out three-dimensional tracing monitoring on the behavior of the Japanese medaka in the area.
The biological behavior sensor manufactured in the embodiment is used for monitoring the behavior periodic change of the Japanese mackerel medaka in normal water, and the specific monitoring test is as follows:
the temperature of the experiment is controlled to be 20 +/-2 ℃, and the illumination period is 16H: and 8D. The running water exposure test was maintained for 4 days. During the test, the flow rate was controlled at 1 liter/hour and the food was fed through the flow system. The behavior periodic change of the Japanese mackerel medaka in normal water is monitored by using a biological behavior sensor.
FIG. 2 shows behavior changes of a Japanese medaka collected by a quadrupole impedance behavior collection system; the abscissa of the graph is time, and the ordinate is the change in the behavioral intensity of a Japanese mackerel medaka. The behavior change of medaka in Japan was within the normal range during the 4-day running water test.
FIG. 3 shows behavior changes of Japanese medaka collected by a three-dimensional tracing behavior collection system. Including analysis of behavior trace, speed, angle, position, etc. of medaka in japan. Thereby judging the behavior change of a Japanese medaka.
After identifying the quadrupole impedance signal and the three-dimensional video signal, analyzing the biological behavior, acquiring the biological behavior signal, converting the acquired virtual signal into a digital signal, and transmitting the digital signal to a biological behavior data processing center. The correlation signals are compared with each other and the change of the biological behavior is analyzed in combination with an environmental step-by-step pressure threshold Model (SBRM) of the biological behavior shown in FIG. 4, so as to infer the change of the water quality condition.
Claims (2)
1. A biological behavior sensor based on quadrupole impedance and three-dimensional tracing is characterized by comprising a flow system, a quadrupole impedance behavior acquisition system, a three-dimensional tracing behavior acquisition system and a data processing center, wherein,
the water flowing system comprises a biological behavior monitoring area, wherein a bottom cover and a top cover are respectively arranged at two ends of the biological behavior monitoring area, a water inlet is formed in the bottom cover, a sensor opening is formed in the top cover, a sensor overflow port is formed in the side face of the sensor opening, and silica gel pads with holes are respectively arranged between the biological behavior monitoring area and the bottom cover and between the biological behavior monitoring area and the top cover;
the quadrupole impedance behavior acquisition system comprises an electrode, the electrode is arranged in a biological behavior monitoring area, an electrode contact is arranged on the electrode, and the electrode contact is connected with special signal identification software through a signal wire;
the three-dimensional tracing behavior acquisition system comprises a camera, wherein the camera is arranged in a biological behavior monitoring area, and is connected with a video signal line which is connected with video signal analysis software;
the quadrupole impedance behavior acquisition system and the three-dimensional tracing behavior acquisition system are connected with the data processing center through digital signals.
2. The monitoring method of the biological behavior sensor based on quadrupole impedance and three-dimensional tracing as claimed in claim 1, comprising the steps of:
a) culturing and selecting the indicator organism;
b) placing an indicator organism into the flow system;
c) a quadrupole impedance behavior acquisition system is used for acquiring signals and transmitting the signals to special signal identification software for analysis and processing;
collecting signals by using a three-dimensional tracing behavior collection system, transmitting the signals to video signal analysis software, and analyzing and processing the signals;
d) and c, transmitting the processing signal in the step c to a data processing center for analysis and judgment.
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Cited By (4)
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| CN107549036A (en) * | 2016-08-02 | 2018-01-09 | 山东师范大学 | Based on electricity, the aquatile behavior on-line monitoring system of vision signal and method |
| CN109633113A (en) * | 2018-12-05 | 2019-04-16 | 环境保护部华南环境科学研究所 | Water quality monitoring method for early warning and system based on medaka behavior model step by step |
| CN109633112A (en) * | 2018-12-05 | 2019-04-16 | 环境保护部华南环境科学研究所 | A method of utilizing polycyclic aromatic hydrocarbon in the variation monitoring water body of medaka intensity of performance |
| CN118464125A (en) * | 2024-07-11 | 2024-08-09 | 吉林大学 | Device and method for testing the response behavior of bioreceptors and bionic components under field stimulation |
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Application publication date: 20121024 |