CN102719485B - A kind of method of Starch Conversion ethanol in root tuber using Momordica grosvenori - Google Patents

Abstract

Wash the roots of Luo Han Guo, slice, mince, extract with petroleum ether, saccharify and filter, bio-ferment, and distill to obtain ethanol products. The method of the invention is simple to operate, greatly reduces the production cost of ethanol, simplifies the production process, greatly improves the utilization rate of starch in the Luo Han Guo tuber, and saves starch resources. The reagents used in the present invention are all non-toxic, cheap and mass-produced chemical reagents, and mature conventional reagent recovery techniques can be used in the whole process, which greatly reduces waste discharge to the environment.

Description

A kind of method utilizing the starch conversion ethanol in Luo Han Guo tuber root

technical field

The invention relates to a method for converting ethanol from starch in Luo Han Guo root tubers, and more specifically relates to a method for directly converting Luo Han Guo tuber root microorganisms into ethanol.

Background technique

Based on my country's national conditions and the current situation of the alcohol industry, the alcohol industry is currently facing three challenges from raw materials, production costs, and clean production processes. In the past few years, my country has used a large amount of aged grains, that is, grains, to produce alcohol. However, it is worth noting that our country has a large population and a small area of cultivated land per capita. The surplus of grain is only a temporary phenomenon. The production of ethanol from sweet potatoes is limited by the planting area, while cassava is used as raw material. Except for a few provinces in my country, cassava is completely dependent on imports, which is costly and unrealistic.

The selection of Luo Han Guo tubers as raw materials for the production of ethanol is in line with the National Development and Reform Commission’s policy that the production of alcohol should not compete with others for food and land; second, the starch content in the roots of Luo Han Guo is high; It is mainly treated as waste and not fully utilized.

Mogroside Ⅴ, mogroside Ⅳ, mogroside Ⅲ, mogroside ⅡE, mogroside ⅢE, mogroside Ⅵ, mogroside A, mogroside, simonoside Ⅰ, 11-oxidized mogroside are mainly isolated from the fruit of Luo Han Guo. Glycoside 11 -oxomogroside, mogroside diol benzoate, amino acid, kaempferol-3,7-α-L dirhamnoside (Ⅱ), mogroside, unsaturated fat, farnesol, mannitol, mogroside A , Mogrosin B, Mogrosin C, Mogrosin D and other substances.

The Chinese patent with the publication number CN1397539 introduces a method for extracting various active ingredients from the residue of Luo Han Guo: the residue after extraction of mogroside is used as raw material, and the method is obtained by extracting seed oil, extracting and fractionating from the seed oil Nisolin is extracted from the water phase extracted from seed oil to obtain fatty aldehyde, and dietary fiber is prepared from the hull or mixed residue after extracting seed oil by chemical or enzymatic methods. The method of the invention is simple and convenient, has a high yield, develops a new raw material source for the production of farnesol, and also greatly promotes the Luo Han Guo industry, and has good economic and social benefits.

The Chinese patent with the publication number CN1436786 introduces a method for extracting mogroside from Luo Han Guo: fresh Luo Han Guo is used as raw material, water is used as the extraction solvent, and microfiltration, ultrafiltration and nanofiltration are used as the main extraction processes, wherein microfiltration The membrane adopts microporous membrane with a pore size of 0.05 μm to 0.3 μm; the ultrafiltration membrane adopts a hollow fiber ultrafiltration membrane with a cut-off relative molecular weight of 20,000 to 80,000; the nanofiltration adopts a nanofiltration membrane with a cut-off relative molecular weight of 100 to 400 or RO reverse osmosis equipment for concentration.

The Chinese patent with publication number CN87101850 introduces a method for extracting Luo Han Guo sweetener: use Luo Han Guo fruit to grind, beat, extract with warm water at 50-60 ° C, filter. Separation by DA-201 acrylic acid type adsorbent, 50% ( V/V) Dissolve in ethanol, decolorize, desalt, and remove impurities with D-211 acrylic anion resin. Recover ethanol by distillation, concentrate under reduced pressure with a vacuum pump, and freeze-dry to obtain white crystalline monk fruit sweetener.

The Chinese patent with the publication number CN1181908 introduces a new process for the production of fresh Luo Han Guo concentrated juice: use a screw press to crush the fresh Luo Han Guo, cook the broken fresh Luo Han Guo to extract the juice with a four-stage cooking process, and use a compound enzyme to make the cooked The pectin in the juice is enzymatically hydrolyzed and precipitated, the juice is clarified, and then the clarified juice is filtered with a plate-and-frame diatomite filter press, and then the juice is sterilized by high-temperature instantaneous sterilization, and concentrated by centrifugal membrane method and vacuum The pot concentrates the juice to 25Be, and finally cools the finished product to room temperature for packaging.

The Chinese patent whose publication number is CN1611498 introduces a kind of Luo Han Guo flavonoids and its preparation process: using water or ethanol as solvent, adopting reflux extraction method, flavonoids are extracted from the leaves and vines of Luo Han Guo, and then resin adsorption method is used to extract the flavonoids The flavonoids in the extract are enriched, eluted with ethanol solution, concentrated, and dried to obtain a crude product containing more than 25% flavonoids; the crude product is extracted with ethanol, filtered, concentrated, and dried to obtain the flavonoid content More than 50% of high-quality Luo Han Guo flavonoids.

The Chinese patent with the publication number CN1634814 introduces a preparation method of Luo Han Guo triterpenes: first, the Luo Han Guo triterpenes extracted from fresh Luo Han Guo are stored at room temperature for more than three years to allow them to age naturally, and then the alcoholized The Luo Han Guo triterpenes were frozen at -10-10°C for 5-40 minutes and taken out, thawed at room temperature and then placed at 80-120°C, heated for 5-40 minutes, taken out and allowed to cool at room temperature That's it.

The Chinese patent with the publication number CN1508139 introduces a mogroside extraction process: crush the Luo Han Guo, add ethanol solution, and soak for 5-15 hours at a temperature of 30-60°C. The filtrate is filtered, the temperature drops to 20-35°C, and the filtrate is sequentially sent to three membrane separation modules through a pressure pump at a working pressure of 0.01-0.2 MPa; the first stage intercepts and separates the concentrated Luo Han Guo fiber, protein, pectin and impurities , used to make organic fertilizers; the second stage is concentrated mogroside solution, which is vacuum dried to obtain mogroside products; the third stage is concentrated mogroside polysaccharides and trace elements, which is mogroside sugar paste; the remaining filtrate is recovered and recycled .

The Chinese patent with the publication number CN1683384 introduces a method and equipment for extracting fresh grosvenor glycosides: wash the fresh grosvenor fruit, crush and squeeze the juice; place the obtained fresh grosvenor fruit juice in a high-pressure precision filtration separation device for processing; the obtained fresh grosvenor fruit The concentrated liquid of Luo Han Guo is dried to obtain fresh mogroside. The method of the present invention uses purely mechanical equipment to extract fresh mogroside, wherein the juice extracting equipment used will only crush the pulp of fresh Luo Han Guo, and will not crush its kernels together , so that the extracted mogroside does not contain the ingredients and taste of the monk fruit kernel, and high-purity monk fruit juice can be obtained.

The Chinese patent with the publication number CN1683387 introduces a method for extracting mogroside from Luo Han Guo: using Luo Han Guo as raw material, extracting, concentrating, separating and purifying the method of extracting mogroside with water or low-carbon alcohol Solvent; the extraction method is water heating extraction or ultrasonic extraction or microwave extraction; separation and purification are column chromatography separation and purification or membrane separation and purification, and the weight content of mogroside in the extract is 40-80%, or the weight content of mogroside V 20-40%.

The Chinese patent with the publication number CN1706861 introduces a preparation method and application of high-purity mogroside V: first crush the grosvenoria grosvenori, heat and reflux with ethanol to extract, then separate the extract with a D101 macroporous resin column, and deionize it first. Water and ethanol were eluted to colorless, and then the eluate was collected, concentrated under reduced pressure to an extract, and then separated by a normal-phase silica gel column, adding chloroform, methanol, and water as eluents, collecting the eluate, and reducing Concentrate under high pressure, then separate with reversed-phase silica gel C-18 column, and use HPLC detection for each eluate collected, combine the same retention time and the purity between 90% ~ 98% and more than 98% of Luo Han Guo The glycoside V component is concentrated under reduced pressure to obtain mogroside V with a purity of 90% to 98% and a mogroside V component with a purity greater than 98%.

The Chinese patent with the publication number CN1854149 introduces a method for extracting mogroside V: take the fresh fruit of Luo Han Guo as raw material, after cleaning, crushing, boiling and extracting, then concentrate the boiling extract, and use macroporous resin for adsorption and separation , carry out gradient cleaning to the macroporous resin, decompress the final cleaning solution to recover ethanol to obtain extract, and dry the extract to obtain the product of the present invention. Taking the weight of the fresh fruit of Luo Han Guo as the base, the output rate of the extract can reach more than 1.1%, and the content of mogroside V in the total amount of the extract can reach 30%-65%.

The Chinese patent with the publication number CN101100481 has introduced a method for extracting tetracyclic triterpenes from Luo Han Guo: first extract the yellow pigment in the fresh Luo Han Guo pericarp with warm water, then heat to make the protein colloidal solidification in the Luo Han Guo fruit cells unable to dissolve, and then break it, It is then extracted with hot water at 60-90°C, the extract is degummed with pectinase, filtered, concentrated, and dried to obtain the crude tetracyclic triterpene.

The Chinese patent with the publication number CN101327244 introduces a decolorized and debittered Luo Han Guo extract and its preparation method: wash the Luo Han Guo, crush and squeeze the juice, centrifuge the juice, put the supernatant into a macroporous adsorption resin, and then water and alkaline solvent Wash the resin with water until the effluent is neutral, then elute with 30% to 65% ethanol, collect the ethanol eluate, recover the ethanol, and dry it to obtain the product.

The Chinese patent with the publication number CN101386636 introduces a method for extracting mogroside: including crushing Luo Han Guo - extracting - absorbing the extract with a macroporous adsorption resin - eluting the resin - collecting the eluate - concentrating and drying; wherein, the The macroporous adsorption resin is ADS-21 type, or ADS-17 type, or ADS-7 type, or ADS-F8 type strong polar macroporous adsorption resin.

The Chinese patent with the publication number CN101440118 introduces a saponin compound extracted from the root of Luo Han Guo, its preparation method and its use: the saponin compound Siraitic acid IIB and Siraitic acid IIC extracted and separated from the root of Luo Han Guo, and its preparation method and its application in anticancer drugs. The molecular formulas of the above two compounds are respectively C ₄₀ H ₆₀ O ₁₄ and C ₄₀ H ₆₀ O ₁₅ . The chemical structures of these two compounds have been confirmed by a variety of modern spectral analysis, especially the comprehensive analysis of two-dimensional nuclear magnetic resonance spectrum. .

The Chinese patent with publication number CN101444333 introduces a preparation method and application of Luo Han Guo extract: 1. dried Luo Han Guo is crushed through a 40-100 mesh sieve; extracted with a mixed solvent and concentrated; the concentrate is subjected to molecular distillation for the first time to obtain Light component 1# and heavy component 2#; heavy component 2# carries out molecular distillation for the second time to obtain light component 3# and heavy component 4#, 4# is the target component; weigh 4# component, use 75 Dissolve % ethanol, spray evenly into blank shredded tobacco, and make cigarettes for smoking; 2. Extraction, ethanol, propylene glycol, and water are mixed solvents in a weight ratio of 2:3:5, and the dosage is 6 to 20 times that of dried Luo Han Guo powder , the extraction time is 1-5h, and the extraction time is 2-4 times; 3. Concentration, the temperature is 90°C, the vacuum degree is 0.098MPa; 4. Molecular distillation, the temperature is 50°C, the vacuum degree is 3000Pa; 5. Molecular distillation, the temperature 80°C, vacuum 46Pa; 6. Addition amount is 0.01%.

The Chinese patent with the publication number CN101690573A introduces a production method of Luo Han Guo extract with mogroside V content greater than 60%: fresh Luo Han Guo undergoes: crushing → saccharification → water extraction → concentration → sedimentation centrifugation → ion exchange resin refining → macroporous resin Refining → concentration → alumina refining → concentration → spray drying → finished product process.

The Chinese patent with the publication number CN101851265A introduces a method for extracting various active ingredients from dried Luo Han Guo: removing the core of the dried Luo Han Guo, then adding water to the peel and pulp, and centrifuging to obtain the sediment and supernatant. The supernatant was subjected to XAD chromatography twice, or polyamide-XAD chromatography, decolorized on the D213 column to obtain sweet glycosides and polyphenols; The polysaccharides and oligosaccharides are precipitated by fractionation; the waste residue is hydrolyzed with polysaccharides, oligosaccharides, and amino acids with gastric enzymes.

The Chinese patent with publication number CN101863946A introduces a method for extracting high-content mogroside V from dried Luo Han Guo: the extraction process includes the following steps: extraction, concentration, filtration, adsorption, decolorization, concentration and drying to obtain a product containing mogroside V .

The Chinese patent with the publication number CN1375499 introduces a method for extracting and separating various components from Luo Han Guo: using water and then treating the broken Luo Han Guo with alkaline water to obtain an extract, and then treating it with fatty alcohol to obtain a precipitate (I) and a solution (II), the precipitate (I) is purified through an anion resin column combined with fatty alcohol treatment to obtain Luo Han Guo polysaccharide. The solution (II) is passed through a polyamide resin column, and the effluent from the column is purified through a macroporous resin column combined with fatty alcohol to obtain mogroside. The components adsorbed by the polyamide resin column are washed out with alkaline fatty alcohol, concentrated and dried to obtain Luo Han Guo flavonoids.

The Chinese patent with the publication number CN1907091 introduces a method for removing the bitterness of the Luo Han Guo extract: ① adding the crude product of the Luo Han Guo extract containing 60% to 99% mogroside into pure water, dissolving and mixing to obtain the Luo Han Guo extract solution ; ② Pass this solution through anion exchange resin and cation exchange resin, or mixed ion exchange resin, or through anion exchange membrane and cation exchange membrane in turn to remove impure substances, because most of the impure substances are adsorbed on the ion exchange On the resin or ion exchange membrane, the obtained light-colored eluent containing mogroside has removed all bitter substances; ③Finally, the powder of Luo Han Guo extract is obtained by spray drying.

The Chinese patent with the publication number CN101147751 introduces a method for extracting biologically active components from Luo Han Guo plants: mechanically pulverize the leaves, vines, roots, stems and fruits of the dried Luo Han Guo plants to obtain coarse powder, or grind fresh Luo Han Guo plants Crush to obtain wet fragments, soak the above coarse powder and fragments with water, or ethanol aqueous solution, or methanol aqueous solution, and heat for extraction to obtain the extract; then filter the extraction mixture, and the filtrate passes through the macroporous adsorption resin at room temperature Separation is carried out by chromatography, and the macroporous adsorption resin column is eluted, and the eluent is collected separately to obtain the desired active part; or the filtrate is separated by silica gel column chromatography, and collected by chloroform/ethanol/water gradient elution. Active components; finally the active components are separated and dried to obtain active substances.

The Chinese patent with the publication number CN101177444 introduces a method for extracting mogroside from Luo Han Guo: fresh or dried Luo Han Guo fruit is used as raw material, water is used as the extraction medium, and multi-stage countercurrent extraction, enzymatic hydrolysis, membrane separation and column chromatography are carried out. The separation and purification process is completed, which solves the problem of impurity removal and separation in the separation of mogroside. It has the characteristics of continuous production, time saving and energy saving, long life of macroporous resin and high product purity. According to the method of the invention, fresh fruit is used as raw material, the extraction yield can reach more than 0.8%, the yield of dried fruit can reach more than 1.8%, and the product purity is 60-90%.

The Chinese patent with publication number CN101200753 introduces a Luo Han Guo extract with mogroside V content ≥ 40% and its preparation method: fresh Luo Han Guo is used as raw material, extracted with water → concentrated → enzymatic hydrolysis → refined by macroporous adsorption resin → ion exchange Resin decolorization→concentration→spray drying.

The Chinese patent with publication number CN101007042 has introduced a kind of preparation method of decolorized and debittered Luo Han Guo extract: use water as a solvent, adopt the reflux extraction method, filter and concentrate the extract, elute with ethanol after decolorizing with decolorizing resin, and use The deodorizing agent deodorizes, recovers ethanol, concentrates, sprays and dries, and pulverizes to obtain decolorized and debittered Luo Han Guo extract. The extract is a white, crystalline powder with pure sweetness and no traditional Chinese medicine taste. Bitter taste, wherein the weight content of mogroside is more than 90%, or the weight content of mogroside V is more than 60%.

The Chinese patent with the publication number CN101029071 introduces a method for preparing high-purity mogroside V from Luo Han Guo: the steps are: (1) first crush the Luo Han Guo into a coarse powder, add ethanol for reflux extraction, and filter to obtain an alcoholic extract; (2) Suspend the alcoholic extract in water, extract 1-3 times with ethyl acetate, and reclaim the ethyl acetate mother liquor; butanol extract; (4) pass the n-butanol extract through a polyamide column, and concentrate the washing solution to obtain crude saponin; (5) conduct silica gel column chromatography on the crude saponin, elute with chloroform-methanol-water, and collect mogroside v Crude product; (6) The crude product of mogroside V was subjected to chromatography, washed with water, and eluted with ethanol to collect mogroside V with a purity greater than 95%.

The Chinese patent with the publication number CN101033244 introduces a method for the purification and preparation of Luo Han Guo alcohol: it includes steps: (1) first crushing Luo Han Guo, adding ethanol to extract to obtain alcoholic extract; (2) suspending the alcoholic extract in water, ethyl acetate Extract and recover ethyl acetate to obtain ethyl acetate extract, and take mother liquor; (3) add hydrochloric acid solution and ethyl acetate to mother liquor, heat for acid hydrolysis; after hydrolysis, separate ethyl acetate layer, adjust to neutral with lye properties, separate ethyl acetate, recover the solvent under reduced pressure, and obtain an extract containing mogrosin; (4) carry out silica gel column chromatography on the extract, elute with chloroform, and recover chloroform; then elute with chloroform-methanol, collect and elute liquid, recovering the solvent to obtain the crude product of mogrosinol; (5) recrystallizing the crude product of mogrosin to obtain mogrosanol with a purity greater than 95%.

The Chinese patent with publication number CN101050230 introduces an extraction and preparation process of Luo Han Guo extract: a method of extracting at 40°C and below and using a combination of multi-stage precision filtration and polyol extraction to simultaneously prepare vitamin C-rich The preparation process of extracts of raw and auxiliary materials for food with main nutritional components and sweetener extracts with main content of mogroside, in which the combination of low-temperature extraction, multi-stage precision filtration and polyol extraction is the key technology related to the present invention.

The Chinese patent with the publication number CN101244112 introduces an application method of the hypoglycemic active ingredient extracted from the Luo Han Guo plant: the hypoglycemic active ingredient extracted from the Luo Han Guo plant is prepared into a drug for lowering blood sugar and treating diabetes, or prepared as a pair Foods with health care effects for hyperglycemia and diabetes patients, the content of hypoglycemic active ingredients in the above products shall not be less than 0.001%. The extracted hypoglycemic active ingredients refer to the sum of the bioactive ingredients extracted from various parts of the whole plant of Luo Han Guo, or the bioactive ingredients extracted from a single part of the plant. The formulation of medicine or health food has hypoglycemic active ingredients, additives and carriers for the product form, and is prepared according to the conventional production methods of these products.

The Chinese patent with the publication number CN101168556 introduces a method for purifying mogroside and extracting biologically active substances: using solvent extraction to purify mogroside and extract "mogroside" at the same time, including liquid preparation and extraction with a moderate non-polar solvent , extraction with medium polar organic solvent, spray drying and other steps.

The Chinese patent with the publication number CN1582701 introduces a low-calorie Luo Han fructose and its manufacturing method: fruit selection, crushing—water extraction—filtering—adsorption—elution—concentration—drying—mixing—packaging Combined crystallization—drying to obtain finished products. Due to the invention of low-calorie monk fructose, Luo Han Guo was extracted with water, separated and removed from insoluble impurities, and then eluted, concentrated, and dried to obtain a Luo Han Guo extract with a relatively high content of mogrosides, and then combined with It is formed by inclusion and recrystallization of plant polysaccharide alcohols.

The Chinese patent with the publication number CN102464685A introduces a method for extracting flavonoid glycosides from the leaves of Luo Han Guo and its products: take the leaves of Luo Han Guo, add water and boil to extract 2 to 3 times, each time for 50 to 100 minutes, filter, combine the filtrates, and enter the large Pore resin adsorption, wash the resin until the effluent is colorless, then elute with ethanol or anhydrous methanol, collect the eluate and remove ethanol or methanol, put it in the refrigerator for 20-30h, precipitate out, dissolve the precipitate with anhydrous ethanol, After filtering, the filtrate was evaporated to dryness to obtain the crude extract of kaempferol-3,7-a-L-dirhamnoside.

The Chinese patent with the publication number CN102408463A introduces a method for extracting mogroside: use HPD-100 type macroporous resin to absorb other components in the Luo Han Guo extract and the strength of mogroside is different, and the sample is loaded at a specific speed. After washing with ethanol solutions of different concentrations and different elution speeds, all the remaining medicinal liquids after elution are combined and spray-dried to obtain the finished product of mogroside. Among them, the weight content of mogrosides is as high as more than 95%, and the weight content of mogroside V is more than 65%.

The Chinese patent with the publication number CN102138958A introduces a process for extracting and purifying the flavonoids of Luo Han Guo plant: ethanol is used as the extraction solvent, and the extract is purified through a macroporous adsorption resin. It is characterized in that it comprises the following steps: drying, chopping, ethanol reflux extraction, filtration and concentration, column adsorption, water elution, ethanol elution, concentration and drying, ethanol dissolution and filtration, concentration and drying.

The Chinese patent with the publication number CN102321503A introduces a preparation method and application of Luo Han Guo concentrate: raw material pretreatment; two petroleum ether reflux extractions: put Luo Han Guo and petroleum ether into an extractor with a stirring device, and perform two extractions. Petroleum ether reflux extraction; drying: collect the Luo Han Guo that has been extracted twice with petroleum ether in the extractor, and dry the Luo Han Guo; twice microwave reflux extraction: throw the dried Luo Han Guo into a mixing device In the microwave extractor, add deionized water and carry out two microwave reflux extractions; Filtration: After mixing the first and second microwave reflux extracts, filter to remove the precipitate; Concentration: The filtered two microwave reflux extracts The mixed solution of the extract is concentrated under reduced pressure to obtain the product Luo Han Guo concentrate.

Publication number is that the Chinese patent of CN101085991A has introduced a kind of method utilizing potato to produce alcohol: discloses a kind of method utilizing potato to produce alcohol, and it comprises the following steps: (1) potato raw material processing; (2) synchronous liquefaction saccharification: add by Bran koji made by mixing Aspergillus oryzae and Rhizopus in a mass ratio of 1:1.5-2.5, the addition amount is 0.02-0.10g/g raw material, the simultaneous cooking and saccharification temperature is 60-85°C, and the time is 30-100min; (3 ) fermentation: add 0.04-0.12 grams of active dry yeast for wine or fresh yeast for wine, 0.06-0.14 grams of ammonium sulfate, and 0.001-0.15 grams of magnesium sulfate per 100 milliliters of fermented mash, and ferment at a temperature of 28-42 ° C for 48-60 hours; (4) After the fermentation is over, distill according to the conventional method to obtain the finished alcohol.

Using the above process, there are the following defects: one is that the energy consumption is large, and the average consumption of standard coal per ton of alcohol is 0.8-1.0 tons; the other is that the utilization rate of starch is low, generally around 80-84%; the third is that it is not suitable to use Luo Han Guo tubers The reason for the production of alcohol is that there are some antibacterial substances in the roots of Luo Han Guo. The starch is different from the starch of the main crops. It has a strong water absorption and water retention during gelatinization, and the viscosity of the paste is high. The ratio of adding water to water is 1:1.8-2.5, which will inevitably affect the starch of Luo Han Guo. Gelatinization, which in turn affects subsequent processes.

Contents of the invention

The technical scheme adopted in the invention comprises: washing the Luo Han Guo tubers, slicing, mincing, petroleum ether extraction, saccharification and filtration, biological fermentation, and distillation to obtain ethanol products.

Therefore, the present invention provides a kind of method utilizing the starch conversion ethanol in Luo Han Guo tuber root, and step comprises:

(1) Wash the Luo Han Guo tuber with water, slice it, and grind it with a grinder;

(2) Mixing Luo Han Guo tuber and sherwood oil in a certain proportion, and standing still for more than 3 hours;

(3) remove the upper stratum petroleum ether part, add aspergillus oryzae and rhizopus fermented liquid, add compound cellulase to carry out starch degradation simultaneously, obtain starch and cellulose hydrolyzate;

(4) Add a certain amount of active dry yeast to its hydrolyzate, then add 0.04% dipotassium hydrogen phosphate and 0.05% ammonium chloride to ferment;

(5) After the fermentation is finished, the fermented liquid is distilled according to the conventional method to obtain the finished product of ethanol.

In one embodiment, the Aspergillus oryzae, Rhizopus and active dry yeast used in steps (3) and (4) are all well-known commonly used microorganisms, and all similar microorganisms can achieve the above effects.

In another embodiment, the volume of sherwood oil is added in step 2, and its volume (L)/Mogros grosvenori tuber (kg) ratio=2-5:1.

In another embodiment, add the volume of Aspergillus oryzae fermented liquid in step 3, its volume (L)/ Luo Han Guo tuber root (kg) ratio=5-30:100; Add the volume of Rhizopus fermented liquid, its volume (L) / Mangosteen root (kg) ratio=10-40:100.

In another embodiment, the weight of active dry yeast is added in step 4, and its weight (kg)/Fructus Luo Han Guo tuber (kg) ratio=5-25:100.

The Aspergillus oryzae and Rhizopus can also be prepared by known methods: from the test tube strains to the Erlenmeyer flask to plant koji, and then to the fermentation liquid of the fermenter for self-preparation. The enzyme activity in self-prepared Aspergillus oryzae and Rhizopus should reach 12U/mL fermentation broth. Active dry yeast and compound cellulase can be purchased directly from the market, wherein the number of active dry yeast is more than 10 ⁹ /g, and the enzyme activity of compound cellulase should reach 5000U/g dry weight.

Technical effect:

1. The raw materials and equipment used in the method of the present invention are common common raw materials and equipment, which avoids the dependence on expensive raw materials and instruments in the industrialized production process, and greatly reduces the production cost;

2. The utilization rate of starch in Luo Han Guo tubers is greatly improved, and starch resources are saved.

3. The method of the present invention is simple to operate, greatly reduces the production cost of ethanol, and simplifies the production process.

4. The reagents used in the present invention are all non-toxic, cheap and mass-produced chemical reagents, and mature reagent recovery conventional technology can be utilized in the whole process, which greatly reduces the discharge of waste to the environment.

Detailed ways

Hereinafter, the present invention will be further described with examples, but it is not limited to any one of these examples or similar examples.

Example 1

Take 200kg of fresh Luo Han Guo roots after washing and removing impurities, slice and mince, add 700L of petroleum ether to mix, and let it stand still for 4 hours; remove the upper layer of petroleum ether, add 20L of Aspergillus oryzae and 30L of Rhizopus fermentation broth at 30°C Carry out starch degradation, add 4kg compound cellulase to carry out cellulose degradation at the same time, obtain starch and cellulose hydrolyzate; Add 20kg active dry yeast and 0.04% dipotassium hydrogen phosphate and 0.05% ammonium chloride to its hydrolyzate to carry out Fermented at 30°C for 60 hours, the alcohol content of the obtained fermented liquid is 15%, and the utilization rate of starch and cellulose reaches 83%. The fermented liquid is distilled according to the conventional method to obtain the finished product of ethanol.

Example 2

Wash and remove impurities from the fresh Luo Han Guo tubers and take 400kg, slice and mince, add 1200L of petroleum ether to mix, and let it stand for 4 hours; remove the upper layer of petroleum ether, add 40L of Aspergillus oryzae and 50L of Rhizopus fermentation broth at 30°C Carry out starch degradation, add 5kg compound cellulase to carry out cellulose degradation at the same time, obtain starch and cellulose hydrolyzate; Add 30kg active dry yeast and 0.04% dipotassium hydrogen phosphate and 0.05% ammonium chloride to its hydrolyzate to carry out Fermented at 30°C for 60 hours, the alcohol content of the obtained fermented liquid is 19%, and the utilization rate of starch and cellulose reaches 85%. The fermented liquid is distilled according to the conventional method to obtain the finished product of ethanol.

Example 3

Take 600kg of fresh Luo Han Guo root after washing and removing impurities, slice and mince, add 2500L of petroleum ether to mix, and let it stand for 4 hours; remove the upper layer of petroleum ether, add 100L of Aspergillus oryzae and 150L of Rhizopus fermentation broth at 30°C Carry out starch degradation, add 5kg compound cellulase to carry out cellulose degradation at the same time, get starch and cellulose hydrolyzate; Add 60kg active dry yeast and 0.04% dipotassium hydrogen phosphate and 0.05% ammonium chloride to its hydrolyzate to carry out Fermented at 30°C for 60 hours, the alcohol content of the obtained fermented liquid is 25%, and the utilization rate of starch and cellulose reaches 89%; the fermented liquid is distilled according to the conventional method to obtain the finished product of ethanol.

Claims (1)

1. the method for Starch Conversion ethanol, step include in a kind of root tuber using Momordica grosvenori：

(1) Momordica grosvenori root tuber is eluted with water, cut into slices, rubbed with pulverizer；

(2) Momordica grosvenori root tuber and petroleum ether are mixed by a certain percentage, it is static to place more than 3 hours, wherein adding oil Volume L/ Momordica grosvenori root tuber kg ratios=2-5 of ether:1；

(3) upper strata oil ether moiety is removed, adds aspergillus oryzae and root arrhizus fermentation liquid, while adds complex cellulase and carries out starch Degraded, obtains starch and cellulosic hydrolysate, wherein adding volume L/ Momordica grosvenori root tuber kg ratios=5-30 of aspergillus oryzae zymotic fluid: 100, add volume L/ Momordica grosvenori root tuber kg ratios=10-40 of root arrhizus fermentation liquid:100

(4) certain active dry yeast is added into its hydrolyzate, adds 0.04% dipotassium hydrogen phosphate and 0.05% ammonium chloride Fermented, wherein adding weight kg/ Momordica grosvenori root tuber kg ratios=5-25 of active dry yeast:100；

(5) after fermentation ends, zymotic fluid is distilled according to conventional method, obtains ethanol finished product.