CN102715377A - Formula of feed for cultivating monochamus alternatus and preparation process - Google Patents
Formula of feed for cultivating monochamus alternatus and preparation process Download PDFInfo
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- CN102715377A CN102715377A CN2012102205952A CN201210220595A CN102715377A CN 102715377 A CN102715377 A CN 102715377A CN 2012102205952 A CN2012102205952 A CN 2012102205952A CN 201210220595 A CN201210220595 A CN 201210220595A CN 102715377 A CN102715377 A CN 102715377A
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- 241001442207 Monochamus alternatus Species 0.000 title claims abstract description 45
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
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- 241000018646 Pinus brutia Species 0.000 claims abstract description 20
- 241000233866 Fungi Species 0.000 claims abstract description 14
- 239000000843 powder Substances 0.000 claims abstract description 13
- 239000002023 wood Substances 0.000 claims abstract description 13
- 239000000463 material Substances 0.000 claims abstract description 9
- 239000002904 solvent Substances 0.000 claims abstract description 9
- 235000015097 nutrients Nutrition 0.000 claims abstract description 6
- 239000000203 mixture Substances 0.000 claims description 20
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 15
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 12
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 12
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 12
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 12
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 11
- 244000144730 Amygdalus persica Species 0.000 claims description 7
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- 241000121220 Tricholoma matsutake Species 0.000 claims description 7
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims description 7
- 229960000583 acetic acid Drugs 0.000 claims description 7
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims description 7
- 239000001632 sodium acetate Substances 0.000 claims description 7
- 235000017281 sodium acetate Nutrition 0.000 claims description 7
- 239000000811 xylitol Substances 0.000 claims description 7
- 235000010447 xylitol Nutrition 0.000 claims description 7
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims description 7
- 229960002675 xylitol Drugs 0.000 claims description 7
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 6
- 244000215068 Acacia senegal Species 0.000 claims description 6
- 229920001817 Agar Polymers 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 239000004471 Glycine Substances 0.000 claims description 6
- 229920000084 Gum arabic Polymers 0.000 claims description 6
- 241000186660 Lactobacillus Species 0.000 claims description 6
- 241000191992 Peptostreptococcus Species 0.000 claims description 6
- 239000000205 acacia gum Substances 0.000 claims description 6
- 235000010489 acacia gum Nutrition 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 235000010419 agar Nutrition 0.000 claims description 6
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 235000019253 formic acid Nutrition 0.000 claims description 6
- 239000012362 glacial acetic acid Substances 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 229940039696 lactobacillus Drugs 0.000 claims description 6
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 6
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 6
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 6
- 229930091371 Fructose Natural products 0.000 claims description 5
- 239000005715 Fructose Substances 0.000 claims description 5
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 5
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 5
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 claims description 5
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 5
- 239000011707 mineral Substances 0.000 claims description 5
- 235000010755 mineral Nutrition 0.000 claims description 5
- 235000016709 nutrition Nutrition 0.000 claims description 5
- 239000004334 sorbic acid Substances 0.000 claims description 5
- 235000010199 sorbic acid Nutrition 0.000 claims description 5
- 229940075582 sorbic acid Drugs 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 5
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 4
- 239000008101 lactose Substances 0.000 claims description 4
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims description 4
- 239000004299 sodium benzoate Substances 0.000 claims description 4
- 235000010234 sodium benzoate Nutrition 0.000 claims description 4
- 238000005516 engineering process Methods 0.000 claims description 3
- 239000004615 ingredient Substances 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims description 2
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- 238000005303 weighing Methods 0.000 claims description 2
- 238000002372 labelling Methods 0.000 claims 1
- 238000011160 research Methods 0.000 abstract description 7
- 230000003031 feeding effect Effects 0.000 abstract description 3
- 238000009472 formulation Methods 0.000 abstract description 3
- 238000011161 development Methods 0.000 abstract description 2
- 238000012545 processing Methods 0.000 abstract description 2
- 241000243771 Bursaphelenchus xylophilus Species 0.000 description 13
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 6
- 239000012467 final product Substances 0.000 description 6
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 241000254173 Coleoptera Species 0.000 description 4
- 241000244615 Ergates faber Species 0.000 description 4
- 241001442208 Monochamus Species 0.000 description 4
- 241000529915 Xylophilus Species 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 235000017557 sodium bicarbonate Nutrition 0.000 description 3
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 3
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- IZPNTDOWOBGHEG-UHFFFAOYSA-M sodium benzene formate Chemical compound [Na+].[O-]C=O.C1=CC=CC=C1 IZPNTDOWOBGHEG-UHFFFAOYSA-M 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
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- 241001481710 Cerambycidae Species 0.000 description 1
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- 235000001018 Hibiscus sabdariffa Nutrition 0.000 description 1
- 235000005205 Pinus Nutrition 0.000 description 1
- 241000218602 Pinus <genus> Species 0.000 description 1
- 235000005291 Rumex acetosa Nutrition 0.000 description 1
- 240000007001 Rumex acetosella Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- BIGPRXCJEDHCLP-UHFFFAOYSA-N ammonium bisulfate Chemical compound [NH4+].OS([O-])(=O)=O BIGPRXCJEDHCLP-UHFFFAOYSA-N 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
一种培养松墨天牛的饲料配方及制备工艺，属于饲料加工技术领域。其特征在于含有松木粉37-55%，菌类2-9%，营养成分3-11%，辅料8-21%，溶剂13-39%。上述一种培养松墨天牛的饲料配方及制备工艺，含有松墨天牛生长发育所需的各种营养物质，能在松墨天牛实验室人工培养上饲养出与自然界中生长无明显区别的松墨天牛，同时能缩短培养周期,保证松墨天牛的产量和质量，该饲料配方饲养效果好且制作简便，安全有效，成本低廉，可以在满足科研实验室人工培养上的需求。The invention discloses a feed formula and a preparation process for cultivating Monochamus alternatus, belonging to the technical field of feed processing. It is characterized in that it contains 37-55% of pine wood powder, 2-9% of fungi, 3-11% of nutrients, 8-21% of auxiliary materials and 13-39% of solvent. The above-mentioned feed formula and preparation process for cultivating Monochamus alternatus contains various nutrients needed for the growth and development of Monochamus alternatus, and it can be raised in the artificial cultivation of Monochamus alternatus laboratory and has no obvious difference from the growth in nature. Monochamus alternatus can shorten the cultivation period and ensure the output and quality of Monochamus alternatus. The feed formula has good feeding effect and is easy to make, safe and effective, and low in cost. It can meet the needs of artificial cultivation in scientific research laboratories.
Description
技术领域 technical field
本发明属于饲料加工技术领域，具体涉及一种培养松墨天牛的饲料配方及制备工艺。 The invention belongs to the technical field of feed processing, and in particular relates to a feed formula and a preparation process for cultivating Monochamus alternatus. the
背景技术 Background technique
松墨天牛是传播松材线虫最主要的媒介昆虫，携带率为62.5％，平均携带量4601-18445条/头，最高可达289000条/头。危害松属的天牛有6种：松墨天牛、小灰长角天牛、褐梗天牛、赤短梗天牛、椎天牛和松皮脊花天牛。目前已证明前5种天牛是松材线虫的携带者。 Monochamus alternatus is the most important vector insect for spreading pine xylophilus, with a carrying rate of 62.5%, an average carrying amount of 4601-18445/head, and a maximum of 289,000/head. There are 6 kinds of long-horn beetles that harm Pinus: long-horned long-horned long-horned long-horned long-beetled long-horned long-horn long-horned long-horned long-horned long-horned long-horned long-horned beetle, long-horned long-horned long-horned long-horned beetle, short-stemmed long-stemmed long-stemmed long-horned beetle, long-stemmed beetle, and long-horned long-horned beetle. So far, the first five species of long beetles have been proved to be carriers of B. xylophilus. the
松墨天牛取食补充营养过程中成为松材线虫的传播媒介，调查研究发现凡是松材线虫发生地，几乎都有松墨天牛存在。丁玉洲等研究发现松墨天牛种群密度与松材线虫发病程度呈正相关，因此防治天牛，降低虫口密度是预防和除治松材线虫病的根本途径。朋金和认为松墨天牛善飞而不愿飞，羽化后很少飞行,自然扩散能力不强,在纯林内活动范围在100m以内,一般1代成虫向外扩散在150m左右。但来燕学等认为松墨天牛能随气流大范围扩散是松材线虫病新疫点最重要的成因机制，必须引起防治工作者的高度重视，并进一步研究认为松墨天牛是引起松树枯萎的主要生物因子，在松树枯萎中发挥了主要作用，其危害性远远超过松材线虫。来燕学通过实验证实松墨天牛是引起松树枯死的主要原因之一，大力防治松墨天牛是保护我国松林的关键。松材线虫体积小，生长在寄主体内，既不能依靠自己的活动侵染新的寄主，也不能像真菌、细菌等微生物具有随气流、雨水传播的能力，此外，一般也不进人土壤，在土壤中转移传播的可能性微乎其微(只有树木根部受伤时，松材线虫才可以从土壤中侵人根部)。所以，只要控制住传播媒介—松墨天牛，也就间接控制住了松材线虫病。目前，松材线虫病的防治研究主要是针对松墨天牛开展的，希望切断其传播途径，有效地降低其传播媒介昆虫松墨天牛的种群密度。因此防治松墨天牛，切断松材线虫的侵染循环是松材线虫病综合治理中的主要技术。 Monochamus alternatus becomes the transmission medium of pine wood nematode in the process of feeding and supplementing nutrition. Investigation and research found that monochamus alternatus exists in almost all places where pine wood nematode occurs. Ding Yuzhou et al. found that the population density of Monochamus alternata was positively correlated with the incidence of pine wood nematode. Therefore, controlling long beetle and reducing the population density is the fundamental way to prevent and eliminate pine wood nematode disease. Peng Jinhe thinks that the longicorn beetle is good at flying but not willing to fly. It seldom flies after eclosion, and its natural dispersal ability is not strong. Its activity range in pure forest is within 100m, and generally the first generation of adults spreads outward at about 150m. However, Lai Yanxue and others believed that the large-scale spread of monochamus alternatus with the airflow is the most important cause of the new epidemic spot of pine wood nematode disease, which must be paid great attention to by the control workers, and further research concluded that monochamus alternatus is the cause of pine wood nematode. The main biological factor of wilt, which plays a major role in pine wilt, is far more harmful than pine xylophilus. Lai Yanxue has proved through experiments that Monochamus alternatus is one of the main reasons for the death of pine trees, and vigorously controlling Monochamus alternatus is the key to protecting my country's pine forests. Pine xylophilus is small in size and grows in the host body. It can neither infect new hosts by its own activities, nor can it spread with airflow and rain like fungi, bacteria and other microorganisms. In addition, it generally does not enter the soil. The possibility of transfer in soil is minimal (B. xylophilus can invade roots from soil only if the roots of the tree are injured). Therefore, as long as the transmission medium, Monochamus alternata, is controlled, pine wood nematode disease will be controlled indirectly. At present, research on the prevention and control of pine wood nematode disease is mainly carried out on monochamus monochamus, hoping to cut off its transmission route and effectively reduce the population density of its vector insect monochamus monochamus. Therefore, the prevention and control of Monochamus alternatus and cutting off the infection cycle of pine wood nematode are the main technologies in the comprehensive management of pine wood nematode disease. the
为保护占森林面积近半的松林健康，避免因松材线虫造成无法挽回的社会、生态和经济损失，应当重视松墨天牛的研究和防治。松墨天牛实验室培养一直是个难题。本发明主要涉及松墨天牛进行人工培养中的饲料难题，解决当前松墨天牛实验室人工培养较难的现状，旨在探寻简便、有效、稳定的室内培养饲料配方，为进一步培养研究和防治松墨天牛提供保障。 In order to protect the health of pine forests, which account for nearly half of the forest area, and avoid irreparable social, ecological and economic losses caused by pine wood nematodes, attention should be paid to the research and control of Monochamus alternatus. The laboratory cultivation of Monochamus alternatus has always been a difficult problem. The present invention mainly relates to the feed problem in the artificial cultivation of Monochamus alternatus, solves the current situation that the artificial cultivation of Monochamus alternatus is difficult in the laboratory, and aims to find a simple, effective and stable indoor cultivation feed formula for further cultivation research and Provide protection for the prevention and control of Monochamus alternatus. the
发明内容 Contents of the invention
针对现有技术中存在的上述问题，本发明的目的在于设计提供一种培养松墨天牛的饲料配方及制备工艺的技术方案，其安全无毒，简便有效，使用方便，成本低廉，饲养效果优良。 For the above-mentioned problems existing in the prior art, the purpose of the present invention is to design and provide a kind of technical scheme of the feed formula and the preparation process of cultivating Monochamus alternatus, which is safe, non-toxic, simple and effective, easy to use, low in cost, and has high feeding effect. excellent. the
所述的一种培养松墨天牛的饲料配方，其特征在于含有重量百分含量的以下物质：松木粉37-55%，菌类2-9%，营养成分3-11%，辅料8-21%，溶剂13-39%； The feed formula for cultivating Monochamus alternatus is characterized in that it contains the following substances in percentage by weight: 37-55% pine wood powder, 2-9% fungi, 3-11% nutritional ingredients, and 8-5% auxiliary materials. 21%, solvent 13-39%;
所述的菌类为酵母菌、乳酸杆菌、马鞍菌、不解糖消化链球菌、松茸、牛肝菌中的一种或一种以上混合物；所述的营养成分为甘氨酸、苯甲酸钠、山梨酸、硫酸铵、磷酸二氢钠、碳酸氢钠、醋酸钠中的一种或一种以上混合物；所述的辅料为果糖、乳糖、葡萄糖、蔗糖、磷酸、硫酸、盐酸、冰醋酸、甲酸、琼脂、木糖醇、阿拉伯胶、桃胶中的一种或一种以上混合物；所述的溶剂为蒸馏水、矿泉水中的一种或一种以上混合物。 The fungus is one or more mixtures of yeast, lactobacillus, saddlebacillus, peptostreptococcus saccharolyticus, matsutake, boletus; the nutritional ingredients are glycine, sodium benzoate, sorbic acid , ammonium sulfate, sodium dihydrogen phosphate, sodium bicarbonate, sodium acetate, or one or more mixtures; the auxiliary materials are fructose, lactose, glucose, sucrose, phosphoric acid, sulfuric acid, hydrochloric acid, glacial acetic acid, formic acid, agar , xylitol, gum arabic, peach gum, one or more mixtures; the solvent is one or more mixtures of distilled water, mineral water.
所述的一种培养松墨天牛的饲料配方，其特征在于所述松木粉的含量为39-53%，优选为41-50%。 The feed formula for cultivating Monochamus alternatus is characterized in that the content of pine wood powder is 39-53%, preferably 41-50%. the
所述的一种培养松墨天牛的饲料配方，其特征在于所述菌类的含量为4-8%，优选5－7%。 The feed formula for cultivating Monochamus alternatus is characterized in that the content of the fungus is 4-8%, preferably 5-7%. the
所述的一种培养松墨天牛的饲料配方，其特征在于所述营养成分的含量为4-10%，优选为6-9%。 The feed formula for cultivating Monochamus alternatus is characterized in that the content of the nutritional components is 4-10%, preferably 6-9%. the
所述的一种培养松墨天牛的饲料配方，其特征在于所述辅料的含量为10-19，优选为13-18%。 The feed formula for cultivating Monochamus alternatus is characterized in that the content of the auxiliary material is 10-19%, preferably 13-18%. the
所述的一种培养松墨天牛的饲料配方，其特征在于所述溶剂的含量为15-37，优选为16-35%。 The feed formula for cultivating Monochamus alternatus is characterized in that the content of the solvent is 15-37%, preferably 16-35%. the
所述的一种培养松墨天牛的饲料配方的制备工艺，特征在于包括以下步骤： A kind of described preparation technology of the feed formulation of cultivating Monochamus alternatus is characterized in that comprising the following steps:
1）按照权利要求1所述比例称取松木粉、菌类、营养成分、辅料、溶剂，备用； 1) Weighing pine wood powder, fungi, nutrients, auxiliary materials and solvents according to the ratio of claim 1, and set aside;
2）将上述原料充分搅拌均匀后的混合物，均打浆粉碎至100-200目，即得松墨天牛的饲料。 2) The mixture obtained by fully stirring the above-mentioned raw materials is beaten and pulverized to 100-200 mesh to obtain the feed of Monochamus alternatus.
上述一种培养松墨天牛的饲料配方及制备工艺，含有松墨天牛生长发育所需的各种营养物质，能在松墨天牛实验室人工培养上饲养出与自然界中生长无明显区别的松墨天牛，同时能缩短培养周期,保证松墨天牛的产量和质量，该饲料配方饲养效果好且制作简便，安全有效，成本低廉，可以在满足科研实验室人工培养上的需求。 The above-mentioned feed formula and preparation process for cultivating Monochamus alternatus contains various nutrients needed for the growth and development of Monochamus alternatus, and it can be raised in the artificial cultivation of Monochamus alternatus laboratory and has no obvious difference from the growth in nature. Monochamus alternatus can shorten the cultivation period and ensure the output and quality of Monochamus alternatus. The feed formula has good feeding effect and is easy to make, safe and effective, and low in cost. It can meet the needs of artificial cultivation in scientific research laboratories. the
本申请文件中涉及的百分含量除另有说明外，其它的均为纯物质的重量百分含量。 Unless otherwise stated, the percentages involved in this application document are all weight percentages of pure substances. the
具体实施方式 Detailed ways
以下通过具体实施例对本发明作进一步说明，并结合药效试验进一步说明本发明的有益效果。 The present invention will be further described below through specific examples, and the beneficial effects of the present invention will be further described in conjunction with drug efficacy tests. the
实施例1 Example 1
在500L反应釜内投入175kg蒸馏水，再投入205kg松木粉，然后边搅拌边加入5kg乳酸杆菌、5kg马鞍菌、5kg不解糖消化链球菌、5kg松茸和5kg牛肝菌，再慢慢投入6kg山梨酸、6kg硫酸铵、6kg磷酸二氢钠、6kg碳酸氢钠和6kg醋酸钠，搅拌半小时，混合均匀，最后加入5kg葡萄糖、6kg蔗糖、6kg磷酸、6kg硫酸、6kg盐酸、6kg冰醋酸、6kg甲酸、6kg琼脂、6kg木糖醇、6kg阿拉伯胶和6kg桃胶，加热搅拌溶解充分后的混合物，打浆粉碎至100目，即成最终产品。 Put 175kg of distilled water into a 500L reactor, then put in 205kg of pine wood powder, then add 5kg of Lactobacillus, 5kg of Saddlebacillus, 5kg of Peptostreptococcus saccharolyticus, 5kg of matsutake and 5kg of boletus, and then slowly put in 6kg of sorrel Acid, 6kg ammonium sulfate, 6kg sodium dihydrogen phosphate, 6kg sodium bicarbonate and 6kg sodium acetate, stir for half an hour, mix well, finally add 5kg glucose, 6kg sucrose, 6kg phosphoric acid, 6kg sulfuric acid, 6kg hydrochloric acid, 6kg glacial acetic acid, 6kg Formic acid, 6kg of agar, 6kg of xylitol, 6kg of gum arabic and 6kg of peach gum, heated and stirred to dissolve the fully dissolved mixture, beaten and pulverized to 100 mesh, the final product.
实施例2 Example 2
在500L反应釜内投入80kg矿泉水，再投入150kg松木粉，然后边搅拌边加入5kg酵母菌、10kg不解糖消化链球菌、10kg松茸和10kg牛肝菌，再慢慢投入5kg甘氨酸、10kg苯甲酸钠、10kg山梨酸、10kg硫酸铵、3kg磷酸二氢钠、3kg碳酸氢钠和4kg醋酸钠，搅拌半小时，混合均匀，最后加入9kg果糖、9kg乳糖、9kg硫酸、9kg盐酸、9kg冰醋酸、9kg甲酸、9kg琼脂、9kg木糖醇、9kg阿拉伯胶和9kg桃胶，加热搅拌溶解充分后的混合物，打浆粉碎至200目，即成最终产品。 Put 80kg of mineral water into a 500L reactor, then put in 150kg of pine wood powder, then add 5kg of yeast, 10kg of peptostreptococcus, 10kg of matsutake and 10kg of boletus while stirring, and then slowly put in 5kg of glycine and 10kg of benzene Sodium formate, 10kg sorbic acid, 10kg ammonium sulfate, 3kg sodium dihydrogen phosphate, 3kg sodium bicarbonate and 4kg sodium acetate, stirred for half an hour, mixed evenly, finally added 9kg fructose, 9kg lactose, 9kg sulfuric acid, 9kg hydrochloric acid, 9kg glacial acetic acid, 9kg of formic acid, 9kg of agar, 9kg of xylitol, 9kg of gum arabic and 9kg of peach gum, heated and stirred to dissolve the fully dissolved mixture, beaten and pulverized to 200 mesh, the final product.
实施例3 Example 3
在500L反应釜内投入90kg蒸馏水和69kg矿泉水，再投入210kg松木粉，然后边搅拌边加入7kg酵母菌、10kg乳酸杆菌、5kg马鞍菌和5kg牛肝菌，再慢慢投入4kg甘氨酸、10kg苯甲酸钠、10kg磷酸二氢钠、5kg碳酸氢钠和5kg醋酸钠，搅拌半小时，混合均匀，最后加入5kg葡萄糖、5kg蔗糖、10kg磷酸、10kg甲酸、10kg琼脂、10kg木糖醇、10kg阿拉伯胶和10kg桃胶，加热搅拌溶解充分后的混合物，打浆粉碎至120目，即成最终产品。 Put 90kg of distilled water and 69kg of mineral water into the 500L reactor, then put in 210kg of pine wood powder, then add 7kg of yeast, 10kg of lactobacillus, 5kg of saddle fungus and 5kg of boletus while stirring, and then slowly put in 4kg of glycine and 10kg of benzene Sodium formate, 10kg sodium dihydrogen phosphate, 5kg sodium bicarbonate and 5kg sodium acetate, stirred for half an hour, mixed evenly, finally added 5kg glucose, 5kg sucrose, 10kg phosphoric acid, 10kg formic acid, 10kg agar, 10kg xylitol, 10kg gum arabic and 10kg of peach gum, heated and stirred to dissolve the fully dissolved mixture, beaten and pulverized to 120 mesh, the final product.
实施例4 Example 4
在500L反应釜内投入138kg蒸馏水，再投入220kg松木粉，然后边搅拌边加入9kg酵母菌、4kg乳酸杆菌、4kg马鞍菌、4kg不解糖消化链球菌、4kg松茸和4kg牛肝菌，再慢慢投入8kg甘氨酸、5kg苯甲酸钠、5kg山梨酸、10kg硫酸铵和10kg醋酸钠，搅拌半小时，混合均匀，最后加入8kg果糖、8kg葡萄糖、8kg蔗糖、8kg磷酸、8kg硫酸、5kg盐酸、10kg冰醋酸、10kg阿拉伯胶和10kg桃胶，加热搅拌溶解充分后的混合物，打浆粉碎至140目，即成最终产品。 Put 138kg of distilled water into the 500L reactor, and then put in 220kg of pine wood powder, then add 9kg of yeast, 4kg of lactobacillus, 4kg of saddle fungus, 4kg of peptostreptococcus, 4kg of matsutake and 4kg of boletus while stirring, and then slowly Slowly add 8kg glycine, 5kg sodium benzoate, 5kg sorbic acid, 10kg ammonium sulfate and 10kg sodium acetate, stir for half an hour, mix well, finally add 8kg fructose, 8kg glucose, 8kg sucrose, 8kg phosphoric acid, 8kg sulfuric acid, 5kg hydrochloric acid, 10kg ice Acetic acid, 10kg of gum arabic and 10kg of peach gum, heated and stirred to dissolve the fully dissolved mixture, beaten and pulverized to 140 mesh, the final product.
实施例5 Example 5
在500L反应釜内投入117kg矿泉水，再投入230kg松木粉，然后边搅拌边加入11kg酵母菌、5kg乳酸杆菌、5kg马鞍菌、5kg不解糖消化链球菌和5kg松茸，再慢慢投入2kg甘氨酸、10kg苯甲酸钠、10kg山梨酸、10kg硫酸铵和10kg磷酸二氢钠，搅拌半小时，混合均匀，最后加入5kg果糖、5kg乳糖、5kg葡萄糖、5kg蔗糖、5kg磷酸、5kg硫酸、10kg盐酸、10kg冰醋酸、10kg甲酸、10kg琼脂和10kg木糖醇，加热搅拌溶解充分后的混合物，打浆粉碎至160目，即成最终产品。 Put 117kg of mineral water into the 500L reactor, then put in 230kg of pine wood powder, then add 11kg of yeast, 5kg of lactobacillus, 5kg of saddle fungus, 5kg of peptostreptococcus and 5kg of matsutake while stirring, and then slowly put in 2kg of glycine . Glacial acetic acid, 10kg formic acid, 10kg agar and 10kg xylitol, heated and stirred to dissolve the fully dissolved mixture, beating and pulverizing to 160 mesh, the final product.
实施例6 Example 6
在500L反应釜内投入99kg蒸馏水，再投入240kg松木粉，然后边搅拌边加入3kg酵母菌、10kg马鞍菌、10kg松茸和10kg牛肝菌，再慢慢投入3kg苯甲酸钠、10kg山梨酸、10kg硫酸铵、10kg磷酸二氢钠和10kg醋酸钠，搅拌半小时，混合均匀，最后加入5kg果糖、10kg乳糖、10kg葡萄糖、10kg磷酸、10kg硫酸、10kg盐酸、10kg冰醋酸、10kg木糖醇和10kg桃胶，加热搅拌溶解充分后的混合物，打浆粉碎至180目，即成最终产品。 Put 99kg of distilled water into the 500L reactor, then put in 240kg of pine wood powder, then add 3kg of yeast, 10kg of saddle fungus, 10kg of matsutake and 10kg of boletus while stirring, and then slowly put in 3kg of sodium benzoate, 10kg of sorbic acid, and 10kg of sulfuric acid Ammonium, 10kg sodium dihydrogen phosphate and 10kg sodium acetate, stir for half an hour, mix well, finally add 5kg fructose, 10kg lactose, 10kg glucose, 10kg phosphoric acid, 10kg sulfuric acid, 10kg hydrochloric acid, 10kg glacial acetic acid, 10kg xylitol and 10kg peach gum , heating and stirring the fully dissolved mixture, beating and pulverizing to 180 mesh, and then the final product.
以下通过试验说明上述松墨天牛的饲料配方的优良效果。 The excellent effect of the feed formula of the above-mentioned Monochamus alternatus is illustrated by experiments below. the
表1表明：实施例1-6对松墨天牛的饲养均有较好的效果，实施例2优于实施例1，实施例3优于实施例4，实施例6优于实施例5。 Table 1 shows: embodiment 1-6 all has better effect to the raising of Monochamus alternatus, embodiment 2 is better than embodiment 1, embodiment 3 is better than embodiment 4, embodiment 6 is better than embodiment 5. the
表2表明：实施例1-6对松材线虫卵孵化均具有较好的效果，实施例2优于实施例1，实施例4优于实施例3，实施例6优于实施例5，与对照组均有显著性差异。 Table 2 shows: embodiment 1-6 all has better effect to pine wood nematode egg hatching, embodiment 2 is better than embodiment 1, embodiment 4 is better than embodiment 3, embodiment 6 is better than embodiment 5, There were significant differences with the control group. the
Claims (7)
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| CN103349183A (en) * | 2013-07-17 | 2013-10-16 | 天津拓华生物科技有限责任公司 | Feed for artificially feeding thyestilla gebleri adults and application thereof |
| CN107156543A (en) * | 2017-05-10 | 2017-09-15 | 福建农林大学 | A kind of man-made feeds of Monochamus alternatus low instar larvae and preparation method thereof |
| CN107410213A (en) * | 2017-05-10 | 2017-12-01 | 福建农林大学 | A kind of method for propagating Monochamus alternatus artificially |
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| CN101983580A (en) * | 2010-10-25 | 2011-03-09 | 南京林业大学 | Forage for artificial breeding of Monochamus alternatus Hope and method for preparing the same |
| CN102217729A (en) * | 2011-04-26 | 2011-10-19 | 福建省林业科学研究院 | Semi-artificial feed for anoplophora chinensis larvae and artificial feeding method |
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| CN101983580A (en) * | 2010-10-25 | 2011-03-09 | 南京林业大学 | Forage for artificial breeding of Monochamus alternatus Hope and method for preparing the same |
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| CN103349183A (en) * | 2013-07-17 | 2013-10-16 | 天津拓华生物科技有限责任公司 | Feed for artificially feeding thyestilla gebleri adults and application thereof |
| CN107156543A (en) * | 2017-05-10 | 2017-09-15 | 福建农林大学 | A kind of man-made feeds of Monochamus alternatus low instar larvae and preparation method thereof |
| CN107410213A (en) * | 2017-05-10 | 2017-12-01 | 福建农林大学 | A kind of method for propagating Monochamus alternatus artificially |
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