CN102697171A - Elaeagnus conferta roxb extract and application thereof - Google Patents

Abstract

The invention relates to elaeagnus conferta roxb extract and application thereof and belongs to the technical field of cigarette production. The elaeagnus conferta roxb extract can be obtained by the following steps: a, immersing just-picked elaeagnus conferta roxbs in 95 percent ethanol by a ratio of 1:5 in preparation; b, extracting 1 kg immersed elaeagnus conferta roxbs and crushing by a machine; c, obtaining 2 kg pectinase to dissolve in 5 kg water, then filtering to prepare enzymatic hydrolyzate for preparation; d, adding the enzymatic hydrolyzate in the crushed elaeagnus conferta roxbs, and then collecting the filtering liquid by filtering the elaeagnus conferta roxbs in a hot reflux at the temperature of 45-55 degrees for 2 h; e, additionally adding 3 kg pure water, and then collecting the filtering liquid again by filtering in a hot reflux at the temperature of 75-85 degrees for 1 h; and f, combining the filtering liquid obtained from the step d and e together, and then obtaining the elaeagnus conferta roxb tincture through the compression and concentration of the filtering liquid. As for the application, the elaeagnus conferta roxb tincture, by weight ratio, about 0.1-0.3 percent is applied to a 100 g cigarette formula leaf group. Moreover, the advantages of improved aroma concentration and richness are provided, and the effects of inhibiting mixed gas and slightly improving the stimulation and aftertaste are also improved.

Description

A kind of goat's milk fruit extract and its application

Technical field:

The invention relates to a goat milk fruit extract and its application, belonging to the technical field of cigarette production. the

Background technique:

The development of low-tar cigarettes is the demand of consumers and the tobacco industry. The reduction of the tar content of cigarettes will lead to lighter smoke flavor and weaker aroma, which is difficult for consumers to accept. Therefore, it is necessary to develop natural functional additives while reducing cigarette tar. the

Goat milk fruit (Elatagnus conteta Roxb.) is also known as dense flower Elagnus conteta Roxb., South Rhododendron, vine Elagnus, and Xishuangbanna, Yunnan is called Daguo Elagnus. It is the mature fruit of Elagnus conteta Roxb. It is a perennial Evergreen climbing shrubs, distributed in the tropical rainforests of Vietnam, Malaysia, India and other places, as well as southern Yunnan and southern Guangxi of my country. The fruit is rich in nutrition, the edible rate is 73.3-91%, the water content per 100 grams of fresh fruit is 90.6%, the crude protein is 2.45%, the crude fat is 2.8%, the total sugar is 5.1%, and the total acid is 1.45%. Goat's milk fruit has high medicinal value, and has the effects of astringent, antidiarrheal, antitussive, and detoxification. Modern pharmacology has proved that feeding mice with goat's milk fruit has a certain curative effect on chronic bronchitis. After the organic acid contained in the fruit is concentrated, it can be taken orally to inhibit intestinal bacteria. the

The present invention carried out research on the extraction method of goat milk fruit, extracted and prepared goat milk fruit tincture as a cigarette additive, analyzed its volatile components by high-resolution mass spectrometry (HRMS), and carried out cigarette addition tests and sensory evaluation smoking tests. After literature search, there is no report about its application in tobacco. the

Invention content:

The object of the present invention is to provide a goat milk fruit extract and its application. the

In the present invention, fresh goat's milk fruit is extracted by cold soaking, enzymatically hydrolyzed and concentrated to obtain the goat's milk fruit extract. The volatile components of goat milk fruit were analyzed by Headspace-GC-MS instrument, and the massLynx V4.1 chemical workstation data processing system was used to search in the Nist2008 and WILYL standard spectral library, and the spectral analysis was carried out in combination with relevant literature to confirm its chemical composition . Quantitative analysis by area normalization method, respectively, to obtain the relative percentage of each chemical component, the detection rate was 59.28%. Goat milk fruit extract of the present invention is obtained through the following steps:

a. Soak the freshly picked goat milk fruit in 95% ethanol at a ratio of 1:5, and set aside;

b. Weigh 1kg of soaked goat milk fruit and break it up mechanically;

c. Weigh 2g of pectinase, dissolve in 5kg of water, filter, prepare enzymatic hydrolysis solution, and set aside;

d. Add the above-prepared enzymatic hydrolysis solution to the mechanically crushed goat milk fruit, reflux at 50°C±5°C for 2 hours, then filter, and collect the filtrate;

e. Then add 3kg of pure water, reflux at 80°C±5°C for 1h, filter and collect the filtrate;

f. Combine the filtrates of steps d and e, and concentrate the filtrate under reduced pressure to obtain goat milk fruit tincture. the

Quantitative analysis is used to obtain the relative percentage of each volatile component by area normalization method. The result is as follows:

The results of the analysis showed that alcohols accounted for 12.19%, aldehydes accounted for 26.08%, saturated hydrocarbon compounds accounted for 3.31%, and esters accounted for 3.08%. The detected substance of the tincture contains a large amount of 5-hydroxymethylfurfural, meso-inositol and a small amount of isosorbide dinitrate, which make the effect and efficacy of the goat milk fruit more significant. Among them, 5-hydroxymethylfurfural has beneficial effects on the human body such as anti-oxidation and improving blood flow, and isosorbide dinitrate is mainly suitable for the treatment of angina pectoris and congestive heart failure. Other different components have different degrees of effect. the

The application of the goat's milk fruit extract (tincture) obtained in the above steps of the present invention in the proportion of 0.1-0.3% by weight in 100g of cigarette formula leaves. the

The invention has the advantages of improving the concentration and richness of aroma, suppressing miscellaneous gas, and slightly improving stimulation and aftertaste. the

Detailed ways:

The extraction steps of the goat milk fruit extract in the examples of the present invention are the same as those described in the summary of the invention, and the instruments used in the examples are all purchased from the market, and the operation of the instruments is also the prior art. the

Example 1:

Application of Goat's Milk Fruit Extract in Cigarette Formula

Sample preparation method: Weigh three 100g identical cigarette formula leaf groups, add the prepared goat milk fruit extract (tincture) to the 100g cigarette formula leaf group in proportions of 0.1%, 0.2%, and 0.3% by weight, Mix evenly, store in airtight storage in a constant temperature and humidity box with a temperature of 22 ℃ ± 2 ℃ and a relative humidity of 60% ± 5%. After 48 hours, make cigarettes with the same specifications of auxiliary materials, and the numbers are as follows:

Small sample experiment table

Carry out sensory evaluation according to GB5606.4-1996 "Cigarette Sensory Technical Requirements", and make a targeted qualitative description of its items. By evaluating the samples, the results are as follows:

Cigarette sensory quality evaluation table

In terms of smell, compared with the control 0#, samples 1#, 2#, and 3#: they emit a faint fruity and sour aroma, which is in harmony with the tobacco aroma, and the intensity increases slightly with the increase of the added amount. The results of sensory evaluation show that compared with the control 0#, samples 1#, 2#, and 3# have increased sour rhyme in aroma, with a touch of fruity aroma, rich aroma and smoke concentration, and miscellaneous The odor and irritation are slightly reduced, but with the increase of the added amount, the fragrance permeability is slightly reduced, the nasal cavity irritation is slightly increased, and the aftertaste will also bring a slight residue. the

Claims (2)

1. a goat milk fruit extract is characterized in that the goat milk fruit extract obtains through the following steps: a. Soak the freshly picked goat milk fruit in 95% ethanol at a ratio of 1:5, and set aside; b. Weigh 1kg of soaked goat milk fruit and break it up mechanically; c. Weigh 2 g of pectinase, dissolve in 5 kg of water, filter, prepare enzymatic hydrolysis solution, and set aside; d. Add the above-prepared enzymatic hydrolysis solution to the mechanically crushed goat milk fruit, reflux at 50°C±5°C for 2 hours, filter, and collect the filtrate; e. Then add 3kg of pure water, reflux at 80°C±5°C for 1h, filter, and collect the filtrate; f. Combine the filtrates of steps d and e, and concentrate the filtrate under reduced pressure to obtain goat milk fruit tincture. 2. the application of goat milk fruit tincture described in claim 1 in the ratio of 0.1-0.3% by weight percentage in 100g cigarette formula leaf group.