CN102676368A - Device for studying driving mechanism of bacteria - Google Patents
Device for studying driving mechanism of bacteria Download PDFInfo
- Publication number
- CN102676368A CN102676368A CN2011100608329A CN201110060832A CN102676368A CN 102676368 A CN102676368 A CN 102676368A CN 2011100608329 A CN2011100608329 A CN 2011100608329A CN 201110060832 A CN201110060832 A CN 201110060832A CN 102676368 A CN102676368 A CN 102676368A
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- CN
- China
- Prior art keywords
- studying
- driving mechanism
- grooves
- bacteria
- bacteria according
- Prior art date
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- 241000894006 Bacteria Species 0.000 title claims abstract description 31
- 238000005260 corrosion Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 230000001580 bacterial effect Effects 0.000 claims description 2
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 claims 1
- 229920005372 Plexiglas® Polymers 0.000 claims 1
- 239000006059 cover glass Substances 0.000 claims 1
- 238000002474 experimental method Methods 0.000 abstract description 4
- 238000005868 electrolysis reaction Methods 0.000 description 8
- 239000007788 liquid Substances 0.000 description 8
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 7
- 239000011521 glass Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 229910052697 platinum Inorganic materials 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000007797 corrosion Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000009830 intercalation Methods 0.000 description 1
- 230000002687 intercalation Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a device for studying a driving mechanism of bacteria. The device comprises a device body, wherein the device body is provided with two grooves used for containing bacterium-containing culture solution, the two grooves are separated by a convex structure, the convex structure is provided with a plurality of hole grooves, and the two grooves are communicated through the hole grooves. Compared with the prior art, the device for studying the driving mechanism of the bacteria has the advantages that the device has a simple structure and is easy to experiment and operate, the experiment precision is high and the like.
Description
Technical field
The present invention relates to a kind of experimental installation, especially relate to a kind of device of studying the bacterium expulsion mechanism.
Background technology
In recent years, carried out big quantity research for the type of drive of robot both at home and abroad, novel drive principle is extremely important for expanding the activity of human beings scope, utilizes bio-energy, and particularly the exploration of miniature organism energy just just begins.Utilizing the energy of miniature organism, is a new research field thereby drive the robot motion through the motion of control miniature organism.The research bacterium depends on microrobot and promotes the robot motion in liquid environmental simulation and experimental technique etc. can promote the development in industries such as health care, environment and chemistry to the use of bacterium motion control and promotion robot.Abroad have and adopt fluid film to observe the device of bacterium expulsion mechanism; Pull out apparatus for placing by four filaments, form a fluid film between making wherein, bacterium moves about in film; It is added electric forming pH gradient fields; Observation bacterium expulsion mechanism, this device receives the influence of environment bigger, causes experiment effect inaccurate.Therefore need find experimental installation a kind of system, that use range is wide.
Summary of the invention
The object of the invention is exactly that a kind of simple in structure, device of being easy to experimental implementation, research bacterium expulsion mechanism that experimental precision is high is provided in order to overcome the defective that above-mentioned prior art exists.
The object of the invention can be realized through following technical scheme: a kind of device of studying the bacterium expulsion mechanism; It is characterized in that; This device comprises device body, offers two on this device body and is used to hold the groove that contains inoculum, separates through ridge structure between two grooves; Offer many hole slots on the described ridge structure, described two grooves are communicated with through hole slot.
Described device body is processed by transparent corrosion resistant material.
Described transparent corrosion resistant material is a synthetic glass.
Described ridge structure is trapezoidal, and the narrow end of an end is wide.
The width of described many hole slots is different.
The width of described many hole slots is 0.05～0.1mm.
The edge of described two grooves respectively has the slit that is used for intercalation electrode.
Described slit is opened in the place apart from ridge structure 1～5mm.
This device also comprises deckglass, and this deckglass covers apparatus surface.
Compared with prior art, the present invention is simple in structure, is easy to experimental implementation, and experimental precision is high, adopts synthetic glass to process, and cooperates microscope stage and electrolyzer, and it is directed mobile in the pH gradient fields to be used for observing bacterium, lays the first stone for utilizing the mikrobe energy.
Description of drawings
Fig. 1 is a structural representation of the present invention;
Fig. 2 is a perspective view of the present invention;
Fig. 3 is the structure for amplifying synoptic diagram of ridge structure.
Embodiment
Below in conjunction with accompanying drawing and specific embodiment the present invention is elaborated.
Shown in Fig. 1～3, a kind of device of studying the bacterium expulsion mechanism, this device comprises device body 1, offers two on this device body 1 and is used to hold the groove 2 that contains inoculum, depth of groove is 0.2mm.Separate through ridge structure 3 between two grooves 2, ridge structure 3 can be separated left and right sides solution, makes both sides keep a pH gradient fields.Ridge structure 3 is trapezoidal, and the narrow end of an end is wide, and a wide end width is 2mm, and a narrow end width is 1mm.Offering many hole slots 5, two grooves 2 on the ridge structure 3 is communicated with through hole slot 5.The width of many hole slots is different, but its width all is limited within 0.05～0.1mm, and the length of many slotted eyes is also different, and its length depends on the width of ridge structure 3.The edge of two grooves 2 respectively has slit 4.The degree of depth of slit 4 is consistent with depth of groove, and the width of slit 4 is 0.1mm.The distance that slit 4 is opened in from ridge structure 3 is 1～5mm place, offers altogether everywhere, and every place offers three slits.When using when of the present invention, the positive and negative electrode of electrolyzer is separately inserted the slit 4 of two grooves 2, can to make electrolysis form pH Gradient distribution tool also different along with the difference of electrode on position.Electrode for electrolysis adopts 50 microns platinum wire electrodes, and the chemical reaction that takes place when considering microsize and the electrolysis of bacterium need select for use superfine inert metal as electrolysis electrode.Device body 1 is processed by synthetic glass, can bacterium power up in the printing opacity microscopically is observed easily before and after the behavior variation.This device also comprises deckglass, and this deckglass covers apparatus surface.Adopt that synthetic glass is little to be processed into, comprise left and right sides groove and center-aisle,, after adding contains the nutrient solution of bacterium, must add a cover deckglass above that for obtaining thin liquid layer so that observe.
Device uses, and may further comprise the steps:
1, device is fixed on the microscope stage
Note necessary holding device level, if there is angle to tilt, then bacterium moves about with liquid, influences observations.
2, in the groove of the left and right sides, add the nutrient solution that contains bacterium
At this moment can add acid, the pH gradient fields that powers up electrolysis formation can be indicated picture analyzing and testing gained pH gradient fields during to electrolysis through the color of acid.
3, add platinum electrode (also can directly add acid-base solution)
Because the microscopic dimensions of bacterium, platinum electrode is selected 50 microns platinum filament for use.
4, covered on device
Disturb for get rid of as far as possible, liquid layer thickness should be more little good more, adds a cover deckglass at this, so that liquid layer evenly scatters, reduces liquid layer thickness.
5, energising electrolysis, adjustment microscopic examination bacterial reaction
Note the size of electrolysis voltage, excessive voltage can produce bubble therein, can not produce bubble in the experimentation; In case generation bubble; Cause observations to lose efficacy because its volume expanded extruding liquid, bacterium move with liquid, and bubble is in case form; Be difficult to discharge, must test again by washing unit.
Claims (9)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100608329A CN102676368A (en) | 2011-03-14 | 2011-03-14 | Device for studying driving mechanism of bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100608329A CN102676368A (en) | 2011-03-14 | 2011-03-14 | Device for studying driving mechanism of bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102676368A true CN102676368A (en) | 2012-09-19 |
Family
ID=46808924
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011100608329A Pending CN102676368A (en) | 2011-03-14 | 2011-03-14 | Device for studying driving mechanism of bacteria |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102676368A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030022269A1 (en) * | 2000-11-08 | 2003-01-30 | Gregory Kirk | Method of monitoring cell motility and chemotaxis |
| CN100340653C (en) * | 2003-04-09 | 2007-10-03 | 艾菲克特细胞研究所股份有限公司 | Cell Chemotaxis Detection Device |
| JP2010252745A (en) * | 2009-04-28 | 2010-11-11 | Nippon Telegr & Teleph Corp <Ntt> | Bacteria analyzer |
| JP2010252746A (en) * | 2009-04-28 | 2010-11-11 | Nippon Telegr & Teleph Corp <Ntt> | Bacteria analyzer |
| JP2010252744A (en) * | 2009-04-28 | 2010-11-11 | Nippon Telegr & Teleph Corp <Ntt> | Bacteria analyzer |
-
2011
- 2011-03-14 CN CN2011100608329A patent/CN102676368A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030022269A1 (en) * | 2000-11-08 | 2003-01-30 | Gregory Kirk | Method of monitoring cell motility and chemotaxis |
| CN100340653C (en) * | 2003-04-09 | 2007-10-03 | 艾菲克特细胞研究所股份有限公司 | Cell Chemotaxis Detection Device |
| JP2010252745A (en) * | 2009-04-28 | 2010-11-11 | Nippon Telegr & Teleph Corp <Ntt> | Bacteria analyzer |
| JP2010252746A (en) * | 2009-04-28 | 2010-11-11 | Nippon Telegr & Teleph Corp <Ntt> | Bacteria analyzer |
| JP2010252744A (en) * | 2009-04-28 | 2010-11-11 | Nippon Telegr & Teleph Corp <Ntt> | Bacteria analyzer |
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| C06 | Publication | ||
| PB01 | Publication | ||
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| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120919 |