CN102660045A - Method for preparing gelatin sponge by using wood frog wracks - Google Patents

Abstract

The invention discloses a method for preparing gelatin sponge from wood frog residue. The method has the advantages of simple process, low cost and short time consumption, and is suitable for industrial production. The method includes: cleaning and chopping, pretreatment, extraction, centrifugation and freeze-drying, which provides a basis for the comprehensive utilization of the wood frog residue and the development of high-value products.

Description

A method for preparing gelatin sponge from wood frog residue

technical field

The invention belongs to the field of comprehensive utilization of agricultural products, and in particular relates to a method for preparing gelatin sponge from wood frog residue.

Background technique

Because the whole body of the wood frog is a treasure, especially the oil of the wood frog is called soft gold, which has multiple health benefits, but most of the remaining remains of the wood frog after taking the oil of the wood frog are wasted. Studies have found that the remains contain a large amount of collagen and are a good source of collagen and gelatin. Gelatin is not only a kind of protein, but also a kind of polymer biomaterial, which has a series of excellent physical properties, and is widely used in food, medicine, washing products, cosmetics and other fields. Gelatin sponge is a spongy solid preparation made of gelatin liquid after foaming, solidification, freeze-drying and sterilization. Due to its superior biocompatibility and safety, biodegradability and weak antigenicity, it makes it Become a superior biomedical material. The preparation of gelatin sponge from the remains of Rana can promote the comprehensive utilization of Rana and increase the added value of Rana.

Contents of the invention

The purpose of the present invention is to provide a method for preparing gelatin sponge from wood frog residue. The method has simple process and is suitable for industrialized production. The method comprises: cleaning and chopping, pretreatment, extraction, centrifugation and freeze-drying.

Cleaning and chopping: Wash and remove the residue of the wood frog and cut it into small pieces.

Pretreatment: Pretreat the fragments of wood frog remains with hydrochloric acid.

Extraction: Heating and extracting the gelatin from the wood frog residue.

Centrifugation: centrifuge to obtain the supernatant to obtain a gelatin solution.

Freeze-drying: The gelatin solution is irradiated with ultraviolet rays and freeze-dried to obtain a collagen sponge.

Detailed ways

Example 1

Cleaning and chopping: Wash and remove the residue of the wood frog and cut it into small pieces of 1 cm.

Pretreatment: Take 50 g of wood frog residue fragments and pretreat them with 1.2% hydrochloric acid for 24 hours at a treatment temperature of 5°C.

Extraction: Heating at 50°C for 5 hours to extract the gelatin from Rana Rana residue.

Centrifugation: centrifuge to obtain the supernatant to obtain a gelatin solution.

Freeze-drying: irradiate the gelatin solution with ultraviolet rays, 365nm, working distance 5, irradiation time 2h, freezing temperature -40°C.

Example 2

Cleaning and chopping: Wash and remove the residue of the wood frog and cut it into small pieces of 1 cm. .

Pretreatment: take 300g and pretreat the wood frog residue fragments with 1.2% hydrochloric acid for 24 hours, and the treatment temperature is 5°C.

Extraction: Heating at 50°C for 5 hours to extract the gelatin from Rana Rana residue.

Pure centrifugation: centrifuge to obtain the supernatant to obtain a gelatin solution.

Freeze-drying: irradiate the gelatin solution with ultraviolet rays, 365nm, working distance 5, irradiation time 2h, freezing temperature -40°C.

Claims (6)

1. the present invention relates to a kind ofly be equipped with the method for gelfoam, it is characterized in that said method comprises the steps: from the residual system of wood frog

A) clean chopping: with being cut into small pieces after the residual body cleaning and removing impurities of wood frog;

B) pre-treatment: with the residual body fragment of hydrochloric acid pre-treatment wood frog;

C) extract: the residual body gelatin of heating and extracting wood frog;

D) centrifugal: the centrifuging and taking supernatant obtains gelatin solution;

E) freeze-drying: gelatin solution is used ultraviolet radiation, and lyophilize obtains gelfoam.

2. according to the described cleaning chopping of claim 1a, it is characterized in that about residual body fragment 1cm.

3. according to the described pre-treatment of claim 1b, it is characterized in that concentration of hydrochloric acid 1.2%, solid-liquid ratio 1: 8 (w/v), treatment time 24h, 5 ℃ of treatment temps.

4. according to the described extraction of claim 1c, it is characterized in that extracting 50 ℃ of temperature, extraction time 5h.

5. described centrifugal according to claim 1d, it is characterized in that centrifugal speed 2500rpm/min, centrifugation time 15min.

6. according to the described freeze-drying of claim 1e, it is characterized in that ultraviolet wavelength 365nm, operating distance 5, irradiation time 2h, freezing temperature-40 ℃.