CN102657240B - Bioactivator capable of inhibiting activity of ring rot pathogen and application thereof - Google Patents
Bioactivator capable of inhibiting activity of ring rot pathogen and application thereof Download PDFInfo
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- CN102657240B CN102657240B CN201210118981.0A CN201210118981A CN102657240B CN 102657240 B CN102657240 B CN 102657240B CN 201210118981 A CN201210118981 A CN 201210118981A CN 102657240 B CN102657240 B CN 102657240B
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Abstract
本发明涉及生物农药领域，具体地，本发明涉及一种具抑制轮纹病菌活性的生物活性物质及其应用。根据本发明的具有抑制轮纹病菌活性的生物活性物质，通过包括以下步骤的方法制备：1)称取独活干燥根粗粉，先用6倍量石油醚浸泡1小时，45℃回流脱脂1小时；2)然后用7-10倍量的75％乙醇回流1-2小时，回收至干；3)用水转移出提取物，萃取得干膏。根据本发明的生物活性物质，其中，在步骤2)中，乙醇回流三次，过滤，合并滤液。本发明的具抑制轮纹病菌活性的生物活性物质具有重要的经济效益和生态效益的双重意义。The invention relates to the field of biological pesticides, in particular, the invention relates to a biologically active substance with the activity of inhibiting ringworm bacteria and its application. According to the biologically active substance with the activity of inhibiting ringworm bacteria of the present invention, it is prepared by a method comprising the following steps: 1) take the dried root powder of lovage, soak it with 6 times the amount of petroleum ether for 1 hour, and reflux and degrease for 1 hour at 45 ° C. ; 2) Then reflux with 7-10 times the amount of 75% ethanol for 1-2 hours, and recover to dryness; 3) Transfer the extract with water, and extract to obtain a dry paste. According to the bioactive substance of the present invention, wherein, in step 2), the ethanol is refluxed three times, filtered, and the filtrates are combined. The biologically active substance with the activity of inhibiting ringworm bacteria of the present invention has dual meanings of important economic benefits and ecological benefits.
Description
Technical field
The present invention relates to field of biological pesticide, particularly, the present invention relates to bioactivator and application thereof that a kind of tool suppresses ring spot bacterium activity.
Background technology
The cultivated area of the apple of China is very large, and the ring rot of apple that ring spot bacterium (Botryosphaeria dothidea) causes is one of a kind of the most serious disease of harm apple production, and is difficult to control, at present still take chemical control as main.Ring rot of apple has generation in China's Main Apple Varieties producing region, when serious, can cause catastrophic collapse to orchard, to Apple Industry, causes heavy losses.The whole vegetative period of apple all can be by infection of Botryosphaeria berengriana f. sp. piricola.Ring spot bacterium also endangers the tree body of apple tree, captures the nutrition of tree body, excretes poison and poisons fruit tree, weakens apple tree vigo(u)r, impact tree body normal growth, and serious causes limb withered, even causes whole tree dead.Moreover, suffer from the limb of ring spot by the bacterium source that becomes generation and again infect newborn apple branch and Apple, cause the generation again of ring spot harm.Ring spot bacterium is also very large to the harm of Apple, and Apple starts morbidity from approaching maturation, and shelf time continues morbidity, causes a large amount of decayed fruits, causes serious direct economic loss.According to expert, report that China just reaches 3,000,000,000 to 5,000,000,000 yuans because ring spot endangers the direct economic loss causing every year.
Ring spot bacterium, except harm apple, can also endanger the fruit trees such as pears, peach, Lee, apricot, grape, chestnut, Malus spectabilis, hawthorn, is the significant threat of above-mentioned fruit production.Ring spot in recent years has to spread and increases the weight of trend, and within 2006, reported first ring spot bacterium can cause bark dieback of European hop hornbean; Within 2010, reported first ring spot bacterium can infect Sweet Osmanthus Leaf Dieback.
All the time, ring rot of apple is all take chemical control as main.Because all can, by infection of Botryosphaeria berengriana f. sp. piricola, therefore need dispenser frequently the whole vegetative period of apple.Because long-term continuous use, ring spot bacterium has produced obvious pesticide resistance, makes the generation of ring spot more and more heavier.Present ring spot bacterium has produced pesticide resistance or drug resistance in various degree to conventional various sterilization agent, and such as thiophanate methyl, mancozeb, anthrax good fortune U.S., the medicament such as raw M-45 has all significantly declined to the control efficiency of ring spot greatly.Long-term continuous use, not only causes germ pesticide resistance, can cause the pollution of the residual and ecotope of fruit Pesticides simultaneously.
Chemical pesticide has become the stumbling-block of agricultural products in China quality safety to the pollution problem of agricultural product.We must recognize the seriousness of this problem clear-headedly, conscientiously ensure agricultural product quality and safety and Environment Ecological Safe.So urgently develop biological agent.
Summary of the invention
The present invention has extracted purifying from the dry root of samphire levisticum (Angelica pubescens Maxim.f.biserrata) bioactivator.
The object of this invention is to provide a kind of bioactivator that suppresses ring spot bacterium activity that has.
A further object of the present invention is to provide the application of above-mentioned bioactivator.
A further object of the present invention is to provide a kind of biopesticide.
According to the bioactivator with inhibition ring spot bacterium activity of the present invention, by the method comprising the following steps, prepare:
1) take the dry root meal of levisticum, first with 6 times of amount benzinums immersion 1 hour, 45 ℃ of backflow degreasings 1 hour;
2) the 75% alcohol reflux 1-2 hour then doubly measuring with 7-10, is recycled to dry;
3) water migrates out extract, and extraction gets dry extract.
According to bioactivator of the present invention, wherein, in step 2) in, alcohol reflux three times, filters merging filtrate.
According to bioactivator of the present invention, wherein, in step 3) in, add chloroform extracting twice, reclaim chloroform, get dry extract.
According to biopesticide of the present invention, wherein, comprise the bioactivator that suppresses ring spot bacterium activity that has of preparing by following steps:
1) take the dry root meal of levisticum, first with 6 times of amount benzinums immersion 1 hour, 45 ℃ of backflow degreasings 1 hour;
2) the 75% alcohol reflux 1-2 hour then doubly measuring with 7-10, is recycled to dry;
3) water migrates out extract, and extraction gets dry extract.
According to the specific embodiment of the present invention, the extraction purge process of bioactivator is as follows:
Take the dry root meal of levisticum, first with 6 times of amount benzinums, soak 1 hour, 45 ℃ of backflow degreasings 1 hour, the 75% alcohol reflux 1-2 hour then doubly measuring with 7-10, totally three times, filtration, merging filtrate, is recycled to dry.Water migrates out extract, adds chloroform extracting twice, reclaims chloroform, gets dry extract.
According to the harm feature of above-described Botryosphaeria berengeriana f. sp, application derives from natural traditional Chinese medicine, and to people, animal safety, bio-chemical control free from environmental pollution more has its outstanding advantage.Therefore the bioactivator of tool inhibition ring spot bacterium activity of the present invention has the double meaning of important economic benefit and ecological benefits.
Accompanying drawing explanation
Fig. 1 has shown biological active matter confrontation Botryosphaeria berengeriana f. sp Toxicity Determination result of the present invention.
Embodiment
Embodiment 1 extracts bioactivator
Take the dry root meal of levisticum, first with 6 times of amount benzinums, soak 1 hour, 45 ℃ of backflow degreasings 1 hour, the 75% alcohol reflux 1-2 hour then doubly measuring with 7-10, totally three times, filtration, merging filtrate, is recycled to dry.Water migrates out extract, adds chloroform extracting twice, reclaims chloroform, gets dry extract.
The active detection of the anti-ring spot bacterium of embodiment 2 bioactivator
Through Yantai, Shandong research of agricultural science institute Plant Protection Institute (the domestic and international agriculture chemical registration field control effectiveness test carrier that the Ministry of Agriculture authenticates) expert appraisal, above-mentioned biological active matter confrontation Botryosphaeria berengeriana f. sp is inhibited.Result of the test is as follows:
Table 1 result shows, biological active matter confrontation Botryosphaeria berengeriana f. sp mycelial growth has certain inhibitory action, its 500 μ gmL
-1actual measurement suppresses growth rate and reaches more than 87%; Method of least squares statistics shows, biological active matter confrontation Botryosphaeria berengeriana f. sp virulence regression equation is y=4.1687+0.6748x, EC
50and EC
90value is respectively 17.0536 μ gmL
-1, 1344.4308 μ gmL
-1.
Table 1SC is to Botryosphaeria berengeriana f. sp mycelial growth virulence
Biological active matter confrontation Botryosphaeria berengeriana f. sp Toxicity Determination as shown in Figure 1.
Claims (3)
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210118981.0A CN102657240B (en) | 2012-04-21 | 2012-04-21 | Bioactivator capable of inhibiting activity of ring rot pathogen and application thereof |
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| CN201210118981.0A CN102657240B (en) | 2012-04-21 | 2012-04-21 | Bioactivator capable of inhibiting activity of ring rot pathogen and application thereof |
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| CN102657240A CN102657240A (en) | 2012-09-12 |
| CN102657240B true CN102657240B (en) | 2014-04-30 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104663745B (en) * | 2015-02-06 | 2018-10-09 | 郎明亮 | A kind of medicament for treating apple tree ring spot |
| CN106490008A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of agricultural chemicals of preventing and treating fruit tree diseases and pests and preparation method thereof |
| CN110150328B (en) * | 2019-05-28 | 2021-05-25 | 山东大学 | Application of radix angelicae pubescentis extract in preventing and treating plant fungal diseases |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101091734A (en) * | 2007-07-05 | 2007-12-26 | 中国药科大学 | Method for preparing extractive of pubescent angelica root coumarin |
| WO2010061938A2 (en) * | 2008-11-25 | 2010-06-03 | Sumitomo Chemical Company, Limited | Composition for controlling plant diseases and method for controlling plant diseases |
| CN101805347A (en) * | 2009-12-04 | 2010-08-18 | 南京泽朗医药科技有限公司 | Method for extracting bergapten from radix angelicae pubescentis |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101091734A (en) * | 2007-07-05 | 2007-12-26 | 中国药科大学 | Method for preparing extractive of pubescent angelica root coumarin |
| WO2010061938A2 (en) * | 2008-11-25 | 2010-06-03 | Sumitomo Chemical Company, Limited | Composition for controlling plant diseases and method for controlling plant diseases |
| CN101805347A (en) * | 2009-12-04 | 2010-08-18 | 南京泽朗医药科技有限公司 | Method for extracting bergapten from radix angelicae pubescentis |
Non-Patent Citations (4)
| Title |
|---|
| 张知侠.短毛独活精油提取及抑菌作用研究.《咸阳师范学院学报》.2007,第22卷(第2期),33-34. |
| 正交试验法优选独活提取工艺;聂诗明 等;《湖北中医杂志》;20040930;第26卷(第9期);51-52 * |
| 短毛独活精油提取及抑菌作用研究;张知侠;《咸阳师范学院学报》;20070430;第22卷(第2期);33-34 * |
| 聂诗明 等.正交试验法优选独活提取工艺.《湖北中医杂志》.2004,第26卷(第9期),51-52. |
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