CN102642993B - Alcohol fermentation wastewater treatment method - Google Patents

Abstract

The invention discloses a treatment method for alcohol fermentation wastewater. It first removes the solid impurities in the alcohol fermentation wastewater, then adjusts the pH value of the alcohol fermentation wastewater to 5.0-8.0, and then inserts the oil yeast seed liquid according to the inoculation amount of 2.5-20% (v/v), and ferments The temperature is 25-35°C, the fermentation time is 2-5 days, and aerobic fermentation is carried out. After the fermentation is completed, the yeast cells in the wastewater are collected, and the wastewater is subjected to subsequent treatment according to the discharge requirements. According to the method of the present invention to treat alcohol fermentation wastewater, not only the treatment efficiency is high, the COD removal rate is high, but also the long-term bacteria domestication stage required by the traditional anaerobic and aerobic activated sludge treatment methods can be exempted, and the production of rich Yeast cells of oil, protein, nucleic acid, lycopene and other high value-added components only need simple and conventional aerobic fermentation equipment, and do not need expensive and complicated process equipment such as anaerobic activated sludge method. The process is simple and the cost is low. low. The whole fermentation process does not need to add any nutrients, and the fermentation period is short.

Description

A kind of treatment method of alcohol fermentation waste water

Technical field:

The invention relates to a treatment method for alcohol fermentation wastewater, in particular to a method for significantly reducing the COD of alcohol fermentation wastewater and simultaneously producing high value-added yeast cells by using oily yeast fermentation.

Background technique:

In recent years, with the depletion of oil resources, human beings have increasingly explored new energy sources. A large amount of biological liquid fuels such as butanol and ethanol can be produced through microbial fermentation technology, which is an effective way to solve the current energy crisis. After fermentation, butanol, ethanol, acetone and other products in the fermentation broth can be recovered by simple distillation technology. However, the remaining fermentation broth is rich in organic acids such as butyric acid and acetic acid, and its COD is often above 4000mg/L, so subsequent wastewater treatment is required to meet the discharge standard.

Oleaginous yeast, a yeast that can use various carbon sources to produce high microbial oil and bacterial protein, has been used in the treatment of various industrial wastewater in recent years. For example, Tan Tianwei's research group at Beijing University of Chemical Technology can use Rhodotorula viscosus to treat industrial wastewater such as monosodium glutamate, starch, and papermaking, and produce microbial oils (Bioresour. Technol. 2010, 101: 6092-5). Venkata Subhash et al. used oily microorganisms to treat corncob wastewater (Bioresour.Technol., 2011, 102(19), 9286-9290). Yousuf et al. used oleaginous yeast to treat olive oil wastewater, etc. (J.Agric.Food Chem., 2010, 58(15), 8630-8635). Different from the above-mentioned fermentation wastewater, alcohol fermentation wastewater is rich in organic acids such as butyric acid and acetic acid, and the COD is often above 4000mg/L. Generally speaking, using an internal circulation anaerobic reactor (IC) and using anaerobic activated sludge to treat this fermented wastewater rich in organic acids can achieve a certain treatment effect. However, this process requires a complex internal circulation anaerobic reactor, and the construction cost is relatively high. Moreover, activated sludge needs several months of domestication to achieve the treatment effect, so the treatment efficiency is low.

At present, there is no document or patent report on the use of oleaginous yeast to treat alcoholic fermentation wastewater.

Invention content:

The object of the present invention is to provide a method that can effectively treat alcohol fermentation waste water, has high treatment efficiency, simple treatment, low cost, short treatment time, and can collect high-addition waste water rich in oil, protein, nucleic acid, lycopene, etc. after fermentation. A method for treating alcoholic fermentation waste water of yeast cells of value products.

The method for treating alcohol fermentation wastewater of the present invention is characterized in that it comprises the following steps of removing solid impurities in alcohol fermentation wastewater, adjusting the pH value of alcohol fermentation wastewater to 5.0 to 8.0, and then adjusting the inoculum size to 2.5 ～20% (v/v) is inserted into the oil yeast seed liquid, the fermentation temperature is 25～35°C, the fermentation time is 2～5 days, and aerobic fermentation is carried out. After the fermentation, the yeast cells in the wastewater are collected. The wastewater is based on Emissions require subsequent treatment.

The said removal of solid impurities in the alcohol fermentation wastewater is preferably to remove the solid impurities in the alcohol fermentation wastewater by filtration or precipitation.

Described lipozyme can be Rhodotorula glutinis, Trichosporoncutaneum, Rhodosporidium toruloides, Lipomyces starkeyi, Cryptococcus albidus ), Trichosporon dermatis and Trichosporn coremiiforme.

The described oleaginous yeast seed liquid is preferably prepared by the following method, inserting the oleaginous yeast strain into the activation medium, 25～35 ℃, activation culture for 24～48 hours, the described activation medium is glucose or xylose 20g/ L, peptone 10g/L, yeast powder 10g/L, and the balance is water.

The yeast cells in the collected wastewater are preferably filtered or centrifuged to collect yeast cells rich in oil, protein, nucleic acid, lycopene and other high value-added components.

The beneficial effects of the present invention are as follows:

1. According to the method of the present invention, alcohol fermentation waste water is processed, not only the treatment efficiency is high, the COD removal rate is high, but also the long-time bacteria domestication stage required by the traditional anaerobic and aerobic activated sludge treatment methods can be avoided, and the production Yeast cells rich in oil, protein, nucleic acid, lycopene and other high value-added components have good application prospects.

2. Oil yeast only needs simple and conventional aerobic fermentation equipment to treat alcohol wastewater, and does not need expensive and complicated process equipment (such as internal circulation reactor, etc.) such as anaerobic activated sludge method. The process is simple and the cost is low.

3. There is no need to add any nutrients during the whole fermentation process, the fermentation cycle is short, and the collection of product yeast cells is simple.

Detailed ways:

The following examples are to further illustrate the present invention, but not to limit the invention.

Example 1:

Treatment of Butanol Fermentation Wastewater Using Rhodotorula glutinis

Insert Rhodotorula glutinis into 100ml of activation medium, and activate and cultivate for 48 hours at 25°C to obtain Rhodotorula glutinis seed liquid. The activation medium is: glucose 20g/L, peptone 10g/L, Yeast powder 10g/L, balance is water.

The butanol fermentation wastewater is filtered to remove solid impurities in the butanol fermentation wastewater. The initial COD of the butanol fermentation wastewater after removing impurities is about 25000mg/L, and the initial pH value of the wastewater is adjusted to 5.0 with acetic acid, with 10% ( The inoculation amount of v/v) was inserted into the seed liquid of Rhodotorula viscosum, and after aerobic fermentation at 28°C for 3 days, the yeast cells in the butanol fermentation wastewater were collected by centrifugation, and the wastewater was subjected to subsequent treatment according to the discharge requirements. The COD removal rate of the butanol-fermented wastewater can reach 75%, and the amount of yeast cells is 5.5g/L.

Example 2

Treatment of Butanol Fermentation Wastewater by Trichosporon cutaneum

Insert Trichosporon cutaneum (Trichosporon cutaneum) into 50ml of activation medium, 35 DEG C, activate culture for 24 hours, obtain Trichosporon cutaneum seed liquid, described activation medium is: xylose 20g/L, Peptone 10g/L, yeast powder 10g/L, and the balance is water.

Precipitate the butanol fermentation wastewater to remove solid impurities in the butanol fermentation wastewater. The initial COD of the butanol fermentation wastewater after removing impurities is about 23000mg/L, and the initial pH value of the wastewater is adjusted to 8.0 with calcium hydroxide. % (v/v) of the inoculum was inserted into the Trichospora dermatosus seed solution, and after 4 days of aerobic fermentation at 28°C, the yeast cells in the butanol fermentation wastewater were collected by centrifugation, and the wastewater was subjected to subsequent treatment according to the discharge requirements. The COD removal rate of the butanol fermentation wastewater after the Trichosporium somatoides fermentation treatment can reach 80%, and the yeast cell volume is 6.5g/L.

Example 3

Treatment of Ethanol Fermentation Wastewater Using Rhodosporidium toruloides

Insert Rhodosporidium toruloides (Rhodosporidium toruloides) into 80ml of activation medium, 30 DEG C, activate culture for 36 hours, obtain Rhodosporidium toruloides seed liquid, described activation medium is: xylose 20g/L, Peptone 10g/L, yeast powder 10g/L, and the balance is water.

The butanol fermentation wastewater is precipitated to remove solid impurities in the butanol fermentation wastewater. The initial COD of the butanol fermentation wastewater after removing impurities is about 12000mg/L, and the initial pH value of the wastewater is adjusted to 6.5 with ammonia water, with 8% ( The inoculation amount of v/v) was inserted into the seed liquid of Rhodosporidium toruloides, and after 2 days of aerobic fermentation at 28°C, the yeast cells in the butanol fermentation wastewater were collected by centrifugation, and the wastewater was subsequently treated according to the discharge requirements. The COD removal rate of the butanol fermentation wastewater treated by the sporozoan yeast fermentation can reach 70%, and the yeast cell volume is 3.8g/L.

Example 4

Treatment of Ethanol Fermentation Wastewater Using Lipomyces starkeyi

Lipomyces starkeyi (Lipomyces starkeyi) was inserted into the activation medium of 150ml, 30 DEG C, activated and cultivated for 36 hours to obtain the seed liquid of Lipomyces starkeyi, and the activation medium was: xylose 20g/L, Peptone 10g/L, yeast powder 10g/L, and the balance is water.

Filter the butanol fermentation wastewater to remove solid impurities in the butanol fermentation wastewater. The initial COD of the butanol fermentation wastewater after removing impurities is about 21000mg/L, and the initial pH value of the wastewater is adjusted to 6.5 with sodium hydroxide. % (v/v) of the inoculum was inserted into the seed liquid of Lipoyeastia studii, and after 5 days of aerobic fermentation at 30°C, the yeast cells in the butanol fermentation wastewater were collected by centrifugation, and the wastewater was subjected to follow-up treatment according to the discharge requirements. The COD removal rate of the butanol fermentation wastewater after the fermentation treatment by L. dablii yeast can reach 78%, and the yeast cell volume is 4.9g/L.

Example 5

Treatment of Butanol Fermentation Wastewater by Cryptococcus albidus

Insert Cryptococcus albidus into 125ml of activation medium, activate and cultivate at 30°C for 36 hours to obtain Cryptococcus albidus seed liquid, the activation medium is: xylose 20g/L, peptone 10g/L 1. Yeast powder 10g/L, and the balance is water.

Filter the butanol fermentation wastewater to remove solid impurities in the butanol fermentation wastewater. The initial COD of the butanol fermentation wastewater after removing impurities is about 24000mg/L, and the initial pH value of the wastewater is adjusted to 7.5 with sodium hydroxide. % (v/v) inoculum was inserted into the seed liquid of Cryptococcus albus, and after aerobic fermentation at 28°C for 4 days, the yeast cells in the butanol fermentation wastewater were collected by centrifugation, and the wastewater was subjected to subsequent treatment according to the discharge requirements. The COD removal rate of the butanol fermentation wastewater after fermentation treatment can reach 65%, and the amount of yeast cells is 3.2g/L.

Example 6

Treatment of Ethanol Fermentation Wastewater Using Trichosporon dermatis

Insert Trichosporon dermatis into 25ml of activation medium, and activate and cultivate for 36 hours at 30°C to obtain Trichosporon dermatis seed liquid. The activation medium is: glucose 20g/L, peptone 10g/L, yeast powder 10g/L, The balance is water.

The butanol fermentation wastewater is filtered to remove solid impurities in the butanol fermentation wastewater. The initial COD of the butanol fermentation wastewater after removing impurities is about 18000mg/L, and the initial pH value of the wastewater is adjusted to 7.8 with sodium hydroxide, and the pH value is adjusted to 2.5 % (v/v) inoculum was inserted into the Trichosporon dermatis seed solution, and after 4.5 days of aerobic fermentation at 25°C, the yeast cells in the butanol fermentation wastewater were collected by centrifugation, and the wastewater was subjected to subsequent treatment according to the discharge requirements, after being fermented by Trichosporon dermatis The COD removal rate of the butanol-fermented wastewater can reach 82%, and the amount of yeast cells is 4.5g/L.

Example 7

Treatment of Butanol Fermentation Wastewater Using Trichosporn coremiiforme

Insert Trichosporn coremiiforme into 80ml of activation medium, and activate and cultivate for 36 hours at 30°C to obtain Trichosporn coremiiforme seed liquid. The activation medium is: glucose 20g/L, peptone 10g/L, yeast powder 10g/L, The balance is water.

Filter the butanol fermentation wastewater to remove solid impurities in the butanol fermentation wastewater. The initial COD of the butanol fermentation wastewater after removal of impurities is about 28000mg/L, and the initial pH value of the wastewater is adjusted to 8.0 with sodium hydroxide. % (v/v) inoculum was inserted into the Trichosporn coremiiforme seed liquid, and after aerobic fermentation at 35°C for 5 days, the yeast cells in the butanol fermentation wastewater were collected by centrifugation, and the wastewater was subjected to subsequent treatment according to the discharge requirements, after being fermented by Trichosporn coremiiforme The COD removal rate of the butanol-fermented wastewater can reach 76%, and the amount of yeast cells is 5.1g/L.

Example 8

Treatment of Butanol Fermentation Wastewater Using Trichosporn coremiiforme

Insert Trichosporn coremiiforme into 80ml of activation medium, 30°C, activate and cultivate for 28 hours to obtain Trichosporn coremiiforme seed liquid, the activation medium is: glucose 20g/L, peptone 10g/L, yeast powder 10g/L, The balance is water.

Butanol fermentation wastewater is filtered to remove solid impurities in butanol fermentation wastewater. The initial COD of butanol fermentation wastewater after removing impurities is about 4000mg/L, and the initial pH value of wastewater is adjusted to 6.5 with sodium hydroxide. % (v/v) inoculum was inserted into the Trichosporn coremiiforme seed liquid, and after aerobic fermentation at 30°C for 5 days, the yeast cells in the butanol fermentation wastewater were collected by centrifugation, and the wastewater was subjected to subsequent treatment according to the discharge requirements, after being fermented by Trichosporn coremiiforme The COD removal rate of the butanol-fermented wastewater can reach 80%, and the amount of yeast cells is 0.81g/L.

Claims (4)

1. the treatment process of an alcohols fermentation waste water, it is characterized in that, comprise the following steps, remove solid impurity in the alcohols fermentation waste water, the pH value of regulating again the alcohols fermentation waste water is 5.0～8.0, then by inoculum size, be volume ratio 2.5～20% access saccharomyces oleaginosus seed liquor, leavening temperature is 25～35 ℃, and fermentation time is 2～5 days, carry out aerobic fermentation, after fermentation ends, collect the yeast thalline in waste water, waste water carries out subsequent disposal according to emission request;

Described saccharomyces oleaginosus is any in rhodotorula glutinis (Rhodotorula glutinis), trichosporon cutaneum (Trichosporon cutaneum), circle red winter spore yeast (Rhodosporidium toruloides), Lipomyces starkeyi (Lipomyces starkeyi), light white latent ball yeast (Cryptococcus albidus), Trichosporon dermatis and Trichosporn coremiiforme.

2. the treatment process of alcohols fermentation waste water according to claim 1, is characterized in that, in described removal alcohols fermentation waste water, solid impurity is to remove solid impurity in the alcohols fermentation waste water by filtering or precipitating.

3. the treatment process of alcohols fermentation waste water according to claim 1, it is characterized in that, described saccharomyces oleaginosus seed liquor is to prepare by the following method, by in saccharomyces oleaginosus bacterial classification access activation medium, 25～35 ℃, activation culture 24～48 hours, described activation medium is glucose or wood sugar 20g/L, peptone 10g/L, yeast powder 10g/L, surplus is water.

4. the treatment process of alcohols fermentation waste water according to claim 1, is characterized in that, the yeast thalline in described collection waste water is by filtering or centrifugal collection.