CN102641342A - Traditional Chinese medicine extract for treating nephropathy and preparation method - Google Patents
Traditional Chinese medicine extract for treating nephropathy and preparation method Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine extract for treating nephropathy and a preparation method, and belongs to the field of traditional Chinese medicine modernization. The traditional Chinese medicine extract for treating nephropathy is a rhubarb root extract which is obtained by extracting rhubarb root medicinal materials or decoction pieces. Research results of the invention show that the rhubarb root extract has good activity for the treatment of glomerulonephritis with respect to diuresis, proteinuria improvement, and hematuria improvement.
Description
Technical field
The invention belongs to modern Chinese traditional medicine field, relate to the new purposes of a kind of Chinese medicine and extract thereof, be specifically related to a kind of Chinese medicine extract of treating nephropathy and preparation method thereof.
Background technology
Radix Rumicis is at the Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae) that has another name called among the people, Radix Rumicis Crispi, Radix Rumicis Patientiae etc., for multiple in the Polygonaceae Rumex (Rumex) can the medicinal plants root and rhizome.Mainly contain Radix Rumicis Japonici, Rumex patientia Linn., Nepal's Radix rumicis acetosae etc., among the people with the medical herbs use, have heat-clearing and toxic substances removing, cooling blood for hemostasis, effects such as killing parasites for relieving itching.The Radix Rumicis aboundresources, but the research for Radix Rumicis both at home and abroad is also very deficient at present, waits us and further goes to excavate.
Document is summarized as follows the main drug effect of Radix Rumicis: (1) anastalsis: Radix Rumicis has the blood coagulation of promotion, reduces vascular fragility, strengthens the effect of blood capillary contractility.These article decoct can make the mice blood clotting time obviously shorten, and through the test of blood capillary method, proves that it can make blood capillary shrink, and permeability reduces.Bufo siccus whole body perfusion experiment shows that it can make vasoconstriction.The composition Polygonum runcinatum var. sinense Hemsl. first of stopping blooding in the Rumex chalepensis Mill is plain, and its anastalsis system promotes the generation of bioprotein composition by small artery is shunk, and maybe be relevant with the promotion progestogen action.The effect of wooden Quercetin and the wooden Quercitroside enhancing capillary resistance that still tool is good in the net fruit acid mould.(2) eliminate the phlegm, cough-relieving and antiasthmatic effect: decocting liquid all has tangible expelling phlegm for arresting cough effect for mouse stomach after the Radix Rumicis root water decoction Deproteinization; Emodin, chrysophanic acid antitussive action are obvious; General anthraquinone is irritated stomach to Cavia porcellus has significantly antiasthmatic effect, and data shows that the nubbin that extracts after emodin, chrysophanol and a large amount of acid also has antiasthmatic effect.(3) antibacterium effect; Emodin in these article root, chrysophanic acid, chrysophanol have inhibitory action in various degree to alpha streptococcus, streptococcus pneumoniae, hemophilus influenza and micrococcus catarrhalis in test tube.In addition, the in vitro tests of bibliographical information emodin also has inhibitory action in various degree to staphylococcus aureus, escherichia coli.The Rumex gmelini Turcz. infusion is only second to oxytetracycline to aureus with inhibition, and is identical with oxytetracycline to the inhibition effect of group B streptococcus, diphtheria corynebacterium.The ethanol extraction of net fruit Folium rumicis acetosae and bright leaf juice all have strong inhibitory action to bacillus ambiguus and Salmonella paratyphi A, salmonella typhi.(4) antifungal, antivirus action: it is respond well that the rumicin in the Radix Rumicis root acts on the dermatosis of red trichophyta, trichophyton interdigitalis and fungal infection.Bibliographical information root tincture has inhibitory action to big sporidiole mycete on husky Bo Shi nutrient chemical, MEC is 1.56-3.12%.Wood Quercitroside and Asia influenza A virus inject Embryo Gallus domesticus, and the prevention infection effect is arranged.(5) antitumor action: curled dock extract, experiment find that tumor is had anti-evil effect, and acidic drug is renderd a service stronger.(6) other effect: data shows anthraquinone derivative tool laxative action, and vitamin B1 can be used as gastrotonica, analeptic; These article also have certain effect of separating painful, calm, diuresis, parasite killing.Decoct ethanol concentration extraction is inhibited to acute lymphoblastic leukemia, acute monocyte type leukemia and subacute type leukaemic hemocyte dehydrogenase, analyzes and finds that its dialogue Cellular respiration has inhibitory action.Remove this, all right stimulating appetite of these article, improvement sleep, raising body resistance against diseases.
At present, there is not the bibliographical information Radix Rumicis extract to be used to treat the purposes of nephropathy.
Summary of the invention
The technical problem that the present invention will solve provides that a kind of therapeutic effect is good, dosage is few, the medicine of the treatment nephropathy of few side effects.
Medicine of the present invention is the Chinese medicine Radix Rumicis extract.
Contain effective ingredient Radix Rumicis general anthraquinone in the Radix Rumicis extract according to the invention, its mass percentage content in extract is 50-90%.
The extracting mode of Radix Rumicis according to the invention is selected from: reflux extraction, steam distillation, supercritical extraction, decocting method, infusion process, percolation, alcohol extraction, water are obtained through refining.
Extract according to the invention is obtained through refining with water through alcohol extraction by the Chinese medicine Radix Rumicis.
Alcohol of the present invention is ethanol.
Radix Rumicis extract of the present invention, preferred manufacturing procedure is following:
(1) fetch earth rhubarb medicinal material or decoction pieces are doubly measured the 60-95% ethanol extraction 2-3 time with 4-8, each 1-3 hour;
(2) after the alcohol extraction medicinal residues are flung to ethanol, extract 2-3 time, each 1-3 hour, merge the water extract with 6-10 times of water gaging;
(3) merge alcohol extract, filter, filtrate to be concentrated into and do not have the alcohol flavor, add an amount of water precipitation, filter, get precipitate A,
(4) supernatant and water extract merge filtration; The polyamide chromatography post of filtrating; The ethanol elution of the 5-20% that water of doubly measuring with resin bed volume 1-5 successively and resin bed volume 2-7 doubly measure is doubly measured the 70-95% ethanol elution with resin bed volume 8-12 at last, collects the 70-95% ethanol elution; Be concentrated into thick extractum, add above-mentioned precipitate A;
(5) an amount of 5-10% sodium hydroxide solution of reuse dissolving filters, and the filtrating adjust pH is to 1-4, and 10 ℃ with held 12-24 hour, filters, and must precipitate drying, promptly gets.
Further preferred, Radix Rumicis extract of the present invention, method for preparing is following:
(1) fetch earth rhubarb medicinal material or decoction pieces with 6 times of amount 90% ethanol extractions 2 times, each 2 hours, filter;
(2) after the alcohol extraction medicinal residues are flung to ethanol, extract 2 times, each 1.5 hours, merge the water extract with 8 times of water gagings;
(3) merge alcohol extract, filter, filtrate to be concentrated into and do not have the alcohol flavor, add an amount of water precipitation, filter, get precipitate A,
(4) supernatant and water extract merge filtration; The polyamide chromatography post of filtrating; Use the water of 2.5 times of amounts of resin bed volume and 15% ethanol elution of 5 times of amounts of resin bed volume successively, use 95% ethanol elution of 10 times of amounts of resin bed volume at last, collect 95% ethanol elution; Be concentrated into thick extractum, add above-mentioned precipitate A;
(5) an amount of 6% sodium hydroxide solution of reuse dissolving filters, filtrating adjust pH to 2.5, and 10 ℃ with held 12-16 hour, filters, and must precipitate drying, promptly gets.
Other preferred manufacturing procedure of the present invention in an embodiment.
Another object of the present invention is to provide a kind of Radix Rumicis extract in order to the preparation of top method.
Another object of the present invention is to provide a kind of pharmaceutical composition that contains Radix Rumicis extract.
Pharmaceutical composition of the present invention can contain the medicine acceptable carrier as required, wherein with Radix Rumicis extract as active constituents of medicine, its shared percentage by weight in preparation can be 0.1-99.9%, all the other are the medicine acceptable carrier.Pharmaceutical preparations composition of the present invention exists with unit dosage form, and said unit dosage form is meant the unit of preparation, as every of tablet, and capsular every capsules, every bottle of oral liquid, every bag of granule, every of injection etc.
Pharmaceutical composition of the present invention can be any pharmaceutically useful dosage form, and these dosage forms comprise: tablet, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, syrup, suck agent, granule, electuary, pill, drop pill, powder, unguentum, sublimed preparation, suspensoid, powder, solution, injection, suppository, ointment, plaster, cream, spray, drop, patch.
Preferably, compositions of the present invention is oral formulations or injection.
Wherein, said oral formulations is selected from a kind of in capsule, tablet, drop pill, granule, concentrated pill, oral liquid and the mixture.
Wherein, said injection is selected from a kind of in injection, lyophilized injectable powder and the aqueous injection.
Pharmaceutical composition of the present invention, the preparation of its oral administration can contain excipient commonly used, such as binding agent, filler, diluent, tablet agent, lubricant, disintegrating agent, coloring agent, flavoring agent and wetting agent, can carry out coating to tablet in case of necessity.
The filler that is suitable for comprises cellulose, mannitol, lactose and other similar filler.Suitable disintegrating agent comprises starch, polyvinylpyrrolidone and starch derivatives, for example sodium starch glycollate.Suitable lubricant comprises, for example magnesium stearate.The acceptable wetting agent of appropriate drug comprises sodium lauryl sulphate.
Pharmaceutical composition of the present invention can be filled through mixing, and the method that tabletting etc. are commonly used prepares solid oral composition.Mix repeatedly active substance is distributed in those compositionss of a large amount of filleies of whole use.
The form of oral liquid for example can be aqueous or oily suspensions, solution, Emulsion, syrup or elixir, perhaps can be a kind of available water before use or other suitable composite dry products of carrier.This liquid preparation can contain conventional additive; Such as suspending agent; For example sorbitol, syrup, methylcellulose, gelatin, hydroxyethyl-cellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenation edible fat; Emulsifying agent, for example lecithin, anhydro sorbitol monooleate or arabic gum; Non-aqueous carrier (they can comprise edible oil), for example almond oil, fractionated coconut oil, such as oily ester, propylene glycol or the ethanol of the ester of glycerol; Antiseptic, for example para hydroxybenzene methyl ester or propyl p-hydroxybenzoate or sorbic acid, and if desired, can contain conventional flavouring agent or coloring agent.
For injection, the liquid unit dosage forms of preparation contains active substance of the present invention and sterile carrier.According to carrier and concentration, can this chemical compound be suspended or dissolving.The preparation of solution is normally through being dissolved in active substance in a kind of carrier filter-sterilized before it is packed into a kind of suitable bottle or ampoule, sealing then.For example a kind of local anesthetic of adjuvant, antiseptic and buffer agent also can be dissolved in this carrier.In order to improve its stability, can be after the bottle of packing into that this compositions is freezing, and under vacuum, water is removed.
Pharmaceutical composition of the present invention; When being prepared into medicament, optionally add suitable medicine acceptable carrier; Said medicine acceptable carrier is selected from: mannitol, sorbitol, sodium pyrosulfite, sodium sulfite, sodium thiosulfate, cysteine hydrochloride, TGA, methionine, vitamin C, EDTA disodium, EDTA calcium sodium, the alkali-metal carbonate of monovalence, acetate, phosphate or its aqueous solution, hydrochloric acid, acetic acid, sulphuric acid, phosphoric acid, aminoacid, sodium chloride, potassium chloride, sodium lactate, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivative, cellulose and derivant thereof, alginate, gelatin, polyvinylpyrrolidone, glycerol, soil temperature 80, agar, calcium carbonate, calcium bicarbonate, surfactant, Polyethylene Glycol, cyclodextrin, beta-schardinger dextrin-, phospholipid material, Kaolin, Pulvis Talci, calcium stearate, magnesium stearate etc.
Another object of the present invention is to provide raw material of Chinese medicine Radix Rumicis or the application of its extract in the medicine of preparation treatment nephropathy.
The present invention also provides a kind of application of pharmaceutical composition in the medicine of preparation treatment nephropathy that contains Radix Rumicis or its extract.
Nephropathy according to the invention comprises: chronic glomerulonephritis, acute glomerulonephritis, latent nephritis, henoch Schonlein purpura nephritis, nephrotic syndrome, diabetic nephropathy, lupus property nephropathy, renal failure, IgA nephropathy and non-IgA nephropathy.
The content assaying method that the Radix Rumicis general anthraquinone also is provided of the present invention.
The mass percentage content of effective ingredient Radix Rumicis general anthraquinone is 50-90% in the Radix Rumicis extract of the present invention.
Total anthraquinones content assay method in the Radix Rumicis extract of the present invention: it is an amount of to get extract, puts in the flask, adds 8% hydrochloric acid solution 10ml, supersound process 2min; Add chloroform 10ml again, reflux 1 hour is put coldly, puts in the separatory funnel; With a small amount of chloroform washing container, obtain the chloroform layer, acid solution reuse chloroform extraction 3 times, each 10ml; Merge chloroform liquid, decompression and solvent recovery is to doing, and residue adds methanol makes dissolving, is transferred in the 10ml measuring bottle; Add methanol and put scale, shake up, microporous filter membrane filter need testing solution; With certain density chrysophanol, emodin, physcione reference substance solution is contrast, and under the 254nm wavelength, the HPLC method is measured three's in the need testing solution content, and three's content sum is the content of general anthraquinone in the extract.
We have done the test of pesticide effectiveness to Radix Rumicis of the present invention or its extract, and result of study shows that Radix Rumicis of the present invention or its extract have good effect aspect brightic treatment, in diuresis, improve albuminuria, improve and all show good active aspect the hematuria; Result of the test shows that also medication effect of the present invention is remarkable, and dosage is few; Instant effect, few side effects, good stability; Preparation technology is simple, and is with low cost, is fit to large-scale production.
The specific embodiment
Through the more detailed explanation the present invention of following specific embodiment, but not as restriction of the present invention.
Embodiment 1, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces with 6 times of amount 90% ethanol extractions 2 times, each 2 hours, filter;
(2) after the alcohol extraction medicinal residues are flung to ethanol, extract 2 times, each 1.5 hours, merge the water extract with 8 times of water gagings;
(3) merge alcohol extract, filter, filtrate to be concentrated into and do not have the alcohol flavor, add an amount of water precipitation, filter, get precipitate A,
(4) supernatant and water extract merge filtration; The polyamide chromatography post of filtrating is used the water of 2.5 times of amounts of resin bed volume and 15% ethanol elution of 5 times of amounts of resin bed volume successively, uses 95% ethanol elution of 10 times of amounts of resin bed volume at last; Collect 95% ethanol elution; Be concentrated into thick extractum, add above-mentioned precipitate A
(5) an amount of 6% sodium hydroxide solution of reuse dissolving filters, filtrating adjust pH to 2.5, and 10 ℃ with held 12-16 hour, filters, and must precipitate drying, promptly gets.
Through measuring, the content of Radix Rumicis general anthraquinone is 80.1% in the extract.
Embodiment 2, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces, with 4 times of amount 60% ethanol extractions 2 times, each 1 hour;
(2) after the alcohol extraction medicinal residues are flung to ethanol, extract 2 times, each 1 hour, merge the water extract with 6 times of water gagings;
(3) merge alcohol extract, filter, filtrate to be concentrated into and do not have the alcohol flavor, add an amount of water precipitation, filter, get precipitate A,
(4) supernatant and water extract merge filtration; The polyamide chromatography post of filtrating; Use 5% ethanol elution of water and 2 times of amounts of resin bed volume of 1 times of amount of resin bed volume successively, with 8 times of amounts of resin bed volume, 70% ethanol elution, collect 70% ethanol elution at last; Be concentrated into thick extractum, add above-mentioned precipitate A;
(5) an amount of 5% sodium hydroxide solution of reuse dissolving filters, filtrating adjust pH to 1, and 10 ℃ with held 12 hours, filters, and must precipitate drying, promptly gets.
Through measuring, the content of Radix Rumicis general anthraquinone is 50.4% in the extract.
Embodiment 3, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces, with 8 times of amount 95% ethanol extractions 3 times, each 3 hours;
(2) after the alcohol extraction medicinal residues are flung to ethanol, extract 3 times, each 3 hours, merge the water extract with 10 times of water gagings;
(3) merge alcohol extract, filter, filtrate to be concentrated into and do not have the alcohol flavor, add an amount of water precipitation, filter, get precipitate A,
(4) supernatant and water extract merge filtration; The polyamide chromatography post of filtrating; Use 20% ethanol elution of water and 7 times of amounts of resin bed volume of 5 times of amounts of resin bed volume successively, with 12 times of amounts of resin bed volume, 95% ethanol elution, collect 95% ethanol elution at last; Be concentrated into thick extractum, add above-mentioned precipitate A;
(5) an amount of 5% sodium hydroxide solution of reuse dissolving filters, filtrating adjust pH to 4, and 10 ℃ with held 14 hours, filters, and must precipitate drying, promptly gets.
Through measuring, the content of Radix Rumicis general anthraquinone is 90.0% in the extract.
Embodiment 4, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces, with 4 times of amount 85% ethanol extractions 2 times, each 1 hour;
(2) after the alcohol extraction medicinal residues are flung to ethanol, extract 2 times, each 2 hours, merge the water extract with 6 times of water gagings;
(3) merge alcohol extract, filter, filtrate to be concentrated into and do not have the alcohol flavor, add an amount of water precipitation, filter, get precipitate A,
(4) supernatant and water extract merge filtration; The polyamide chromatography post of filtrating; Use 5% ethanol elution of water and 2 times of amounts of resin bed volume of 1 times of amount of resin bed volume successively, with 8 times of amounts of resin bed volume, 70% ethanol elution, collect 70% ethanol elution at last; Be concentrated into thick extractum, add above-mentioned precipitate A;
(5) an amount of 5% sodium hydroxide solution of reuse dissolving filters, filtrating adjust pH to 1, and 10 ℃ with held 16 hours, filters, and must precipitate drying, promptly gets.
Through measuring, the content of Radix Rumicis general anthraquinone is 65.5% in the extract.
Embodiment 5, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces, with 8 times of amount 75% ethanol extractions 3 times, each 3 hours;
(2) after the alcohol extraction medicinal residues are flung to ethanol, extract 3 times, each 3 hours, merge the water extract with 10 times of water gagings;
(3) merge alcohol extract, filter, filtrate to be concentrated into and do not have the alcohol flavor, add an amount of water precipitation, filter, get precipitate A,
(4) supernatant and water extract merge filtration; The polyamide chromatography post of filtrating; Use 20% ethanol elution of water and 7 times of amounts of resin bed volume of 5 times of amounts of resin bed volume successively, with 12 times of amounts of resin bed volume, 95% ethanol elution, collect 95% ethanol elution at last; Be concentrated into thick extractum, add above-mentioned precipitate A;
(5) an amount of 5% sodium hydroxide solution of reuse dissolving filters, filtrating adjust pH to 4, and 10 ℃ with held 18 hours, filters, and must precipitate drying, promptly gets.
Through measuring, the content of Radix Rumicis general anthraquinone is 61.3% in the extract.
Embodiment 6, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces, with 6 times of amount 85% ethanol extractions 2 times, each 2 hours;
(2) after the alcohol extraction medicinal residues are flung to ethanol, extract 2 times, each 2 hours, merge the water extract with 8 times of water gagings;
(3) merging alcohol extract, being concentrated into does not have the alcohol flavor, adds an amount of water precipitation, filters, and gets precipitate A,
(4) supernatant and water extract merge filtration; The polyamide chromatography post of filtrating; Use 10% ethanol elution of water and 4 times of amounts of resin bed volume of 3 times of amounts of resin bed volume successively, with 10 times of amounts of resin bed volume, 85% ethanol elution, collect 85% ethanol elution at last; Be concentrated into thick extractum, add above-mentioned precipitate A;
(5) an amount of 7% sodium hydroxide solution of reuse dissolving filters, filtrating adjust pH to 2, and 10 ℃ with held 20 hours, filters, and must precipitate drying, promptly gets.
Through measuring, the content of Radix Rumicis general anthraquinone is 72.6% in the extract.
Embodiment 7, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces, with 6 times of amount 85% ethanol extractions 2 times, each 2 hours;
(2) after the alcohol extraction medicinal residues are flung to ethanol, extract 2 times, each 2 hours, merge the water extract with 8 times of water gagings;
(3) merge alcohol extract, filter, filtrate to be concentrated into and do not have the alcohol flavor, add an amount of water precipitation, filter, get precipitate A,
(4) supernatant and water extract merge filtration; The polyamide chromatography post of filtrating; Use 10% ethanol elution of water and 4 times of amounts of resin bed volume of 3 times of amounts of resin bed volume successively, with 10 times of amounts of resin bed volume, 85% ethanol elution, collect 85% ethanol elution at last; Be concentrated into thick extractum, add above-mentioned precipitate A;
(5) an amount of 7% sodium hydroxide solution of reuse dissolving filters, filtrating adjust pH to 3, and 10 ℃ with held 24 hours, filters, and must precipitate drying, promptly gets.
Through measuring, the content of Radix Rumicis general anthraquinone is 70.8% in the extract.
Embodiment 8, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces, with 5 times of amount 95% ethanol extractions 3 times, each 2 hours;
(2) after the alcohol extraction medicinal residues are flung to ethanol, extract 2 times, each 2 hours, merge the water extract with 7 times of water gagings;
(3) merge alcohol extract, filter, filtrate to be concentrated into and do not have the alcohol flavor, add an amount of water precipitation, filter, get precipitate A,
(4) supernatant and water extract merge filtration; The polyamide chromatography post of filtrating; Use 15% ethanol elution of water and 5 times of amounts of resin bed volume of 3 times of amounts of resin bed volume successively, with 11 times of amounts of resin bed volume, 95% ethanol elution, collect 95% ethanol elution at last; Be concentrated into thick extractum, add above-mentioned precipitate A;
(5) an amount of 7% sodium hydroxide solution of reuse dissolving filters, filtrating adjust pH to 1, and 10 ℃ with held 14 hours, filters, and must precipitate drying, promptly gets.
Through measuring, the content of Radix Rumicis general anthraquinone is 85.6% in the extract.
Embodiment 9, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces, with 5 times of amount 95% ethanol extractions 3 times, each 2.5 hours;
(2) after the alcohol extraction medicinal residues are flung to ethanol, extract 2 times, each 2.5 hours, merge the water extract with 7 times of water gagings;
(3) merge alcohol extract, filter, filtrate to be concentrated into and do not have the alcohol flavor, add an amount of water precipitation, filter, get precipitate A,
(4) supernatant and water extract merge filtration; The polyamide chromatography post of filtrating; Use 15% ethanol elution of water and 5 times of amounts of resin bed volume of 3 times of amounts of resin bed volume successively, with 11 times of amounts of resin bed volume, 85% ethanol elution, collect 85% ethanol elution at last; Be concentrated into thick extractum, add above-mentioned precipitate A;
(5) an amount of 9% sodium hydroxide solution of reuse dissolving filters, filtrating adjust pH to 1, and 10 ℃ with held 14 hours, filters, and must precipitate drying, promptly gets.
Through measuring, the content of Radix Rumicis general anthraquinone is 80.2% in the extract.
Embodiment 10, extract of the present invention
(1) fetch earth rhubarb medicinal material or decoction pieces, with 5 times of amount 95% ethanol extractions 3 times, each 1.5 hours;
(2) after the alcohol extraction medicinal residues are flung to ethanol, extract 2 times, each 1.5 hours, merge the water extract with 9 times of water gagings;
(3) merge alcohol extract, filter, filtrate to be concentrated into and do not have the alcohol flavor, add an amount of water precipitation, filter, get precipitate A,
(4) supernatant and water extract merge filtration; The polyamide chromatography post of filtrating; Use 15% ethanol elution of water and 6 times of amounts of resin bed volume of 3 times of amounts of resin bed volume successively, with 11 times of amounts of resin bed volume, 95% ethanol elution, collect 95% ethanol elution at last; Be concentrated into thick extractum, add above-mentioned precipitate A;
(5) an amount of 5% sodium hydroxide solution of reuse dissolving filters, filtrating adjust pH to 2, and 10 ℃ with held 16 hours, filters, and must precipitate drying, promptly gets.
Through measuring, the content of Radix Rumicis general anthraquinone is 83.1% in the extract.
Embodiment 11, capsule of the present invention
Get any 1 Radix Rumicis extract among the embodiment of the invention 1-10, add an amount of lactose and process capsule.
Embodiment 12, tablet of the present invention
Get any 1 Radix Rumicis extract among the embodiment of the invention 1-10, add an amount of dextrin and process tablet.
Embodiment 13, granule of the present invention
Get any 1 Radix Rumicis extract among the embodiment of the invention 1-10, add an amount of carboxymethyl cellulose, 60 ℃ of forced air dryings are granulated, and granulate promptly gets granule.
Embodiment 14, drop pill of the present invention
Get any 1 Radix Rumicis extract among the embodiment of the invention 1-10, add an amount of Polyethylene Glycol, mix homogeneously, fusion, last drop pill machine is processed drop pill.
Embodiment 15, oral cavity disintegration tablet of the present invention
Get any 1 Radix Rumicis extract among the embodiment of the invention 1-10, add 5% polyvinylpolypyrrolidone, 0.1% magnesium stearate, 50% microcrystalline Cellulose; Process soft material with an amount of alcoholic solution, granulate, 60 ℃ of forced air dryings are granulated; Granulate, compacting promptly gets oral cavity disintegration tablet in flakes.
Embodiment 16, capsule of the present invention
Get any 1 Radix Rumicis extract among the embodiment of the invention 1-10, add appropriate amount of starch, sucrose and magnesium stearate are granulated, and incapsulate, and promptly get capsule.
Embodiment 17, tablet of the present invention
Get any 1 Radix Rumicis extract among the embodiment of the invention 1-10, add appropriate amount of starch, sodium carboxymethyl cellulose, Pulvis Talci mix homogeneously are granulated, and tabletting promptly gets tablet.
Embodiment 18, oral liquid of the present invention
Get any 1 Radix Rumicis extract among the embodiment of the invention 1-10, add an amount of syrup 4g, be dissolved in the pure water of 100ml, homogenizing filters, and the process high-temperature short-time sterilization (135 ℃, 4s).Sterile filling, packing make oral liquid.
Embodiment 19, injectable powder of the present invention
Get any 1 Radix Rumicis extract among the embodiment of the invention 1-10, add an amount of glucose 4.5g, sodium thiosulfate 0.9g and distilled water 1ml, behind the said components mix homogeneously, lyophilization, packing promptly gets injectable powder.
The pharmacodynamic experiment of embodiment 20, extract of the present invention
Zoopery
Test the influence of a Radix Rumicis extract to normal rat urine amount
[experiment material]
1. laboratory animal: 70 of SPF level SD rats, male, body weight 180～220g.Purchase dimension tonneau China company in Beijing.The raising condition: raise in air-conditioned animal housing, temperature is 20 ℃～25 ℃, relative humidity 60%; 5 in every cage, lighting hours 12 hours is added feedstuff at regular time and quantity; Edible Mus special feed (feed corporation,Ltd of Beijing section Australia association produces) is freely drunk water, and changes bedding and padding every day
2. main agents and medicine:
2.1 reagent: normal saline: the blue pharmaceutcal corporation, Ltd in Tianjin, Tianjin, lot number: 09110904
2.2 medicine:
1. receive the reagent thing: Radix Rumicis extract (extract that the embodiment of the invention 1 prepares)
2. positive drug: hydrochlorothiazide; Tianjin Lisheng Pharmaceutical Co., Ltd.; Lot number: 0711018
3. experimental apparatus:
Metabolic cage: Suzhou City Feng Laboratory Animal Equipment Co., Ltd
[experimental technique]
1. preliminary election: get 180g-200g SD healthy male rat, adapt to and raised 1 day, fasting can't help water 24 hours before the experiment, after the filling stomach gives the 25ml/kg normal saline, collected 2 hours urine amounts, reached the 40% above person who irritates the stomach amount with voided volume and was selected into experiment.
2. divide into groups:
Get the qualified rat of preliminary election by the body weight random packet, be respectively the blank group, Radix Rumicis extract high and low dose group, and hydrochlorothiazide group (positive controls), 10 every group.Dosage gets according to the clinical administration dosage conversion of Radix Rumicis and hydrochlorothiazide tablet, is respectively each preparation high dose group 1000mg/kg, each preparation low dose group 500mg/kg, and hydrochlorothiazide group 16mg/kg, blank control group is irritated the stomach normal saline.
3. diuresis experiment: the filling stomach gives 50ml/kg and comprises and loaded by the normal saline of reagent thing before experiment; Compel the rat hypogastric region with hand and make it drain surplus urine, normal control group (being blank control group) gives corresponding normal saline, puts into metabolic cage; One in every cage; Per hour collect urine once, continuous 5 hours, write down per hour urine amount and total volume of urine.
4. statistical method:
When initial data is put in order; With 95% confidence interval definition abnormal data, be about to numerical value and reject above
extraneous data.
Adopt SPSS11.5 software to carry out statistical analysis.Measurement data representes that with
each drug group compares with model group; Adopt one factor analysis of variance, to estimate the effect of medicine.There is the significance meaning P＜0.05 for difference.
[experimental result]
1. compare with blank control group, the positive drug hydrochlorothiazide can significantly increase rat urine amount (P＜0.01); The Radix Rumicis extract high dose group can obviously increase by 5 hours voided volume of rat, and difference has statistical significance (P＜0.01); Though low dose group rat urine amount increases with blank control group more to some extent, difference does not have statistical significance.Concrete outcome sees table 1 for details
Table 1 Radix Rumicis extract is to the influence
of normal rat urine amount
Compare with blank control group:
*: P＜0.05;
*: P＜0.01
Test of the influence of two Radix Rumicis extracts to acute renal failure rat urine amount due to the glycerol
[experiment material]
1. laboratory animal: 70 of SPF level SD rats, male, body weight 180～220g.Purchase dimension tonneau China company in Beijing.The raising condition: raise in air-conditioned animal housing, temperature is 20 ℃～25 ℃, relative humidity 60%; 5 in every cage, lighting hours 12 hours is added feedstuff at regular time and quantity; Edible Mus special feed (feed corporation,Ltd of Beijing section Australia association produces) is freely drunk water, and changes bedding and padding every day
2. main agents and medicine:
2.1 reagent:
1. glycerol: Tianjin chemical reagent company limited; Analytical pure; Lot number: 20070312
2. normal saline: the blue pharmaceutcal corporation, Ltd in Tianjin, Tianjin, lot number: 09110904
2.2 medicine:
1. receive the reagent thing: Radix Rumicis extract (extract that the embodiment of the invention 1 prepares)
2. positive drug: hydrochlorothiazide; Tianjin Lisheng Pharmaceutical Co., Ltd.; Lot number: 0711018
3. experimental apparatus:
Metabolic cage: Suzhou City Feng Laboratory Animal Equipment Co., Ltd
[experimental technique]
1. preliminary election: with experiment one
2. administration and method for preparation of drug: with experiment one
3. diuresis experiment: each organizes rat intramuscular injection 50% glycerol normal saline solution 10ml/kg modeling respectively, and fasting after the modeling (can't help water) is irritated stomach and given 50ml/kg and comprise and loaded by the normal saline of reagent thing after 24 hours; Compel the rat hypogastric region with hand and make it drain surplus urine, model group gives corresponding normal saline, puts into metabolic cage; One in every cage; Per hour collect urine once, continuous 5 hours, write down per hour urine amount and total volume of urine.
4. statistical method: with experiment one
[experimental result]
With model group relatively, each group urine amount all increases to some extent, and difference has the significance meaning, the prompting medicine has certain diuresis to rat under the pathological state, but the difference between each administration group does not have statistical significance.Concrete data see table 2 for details
Table 2 Radix Rumicis extract is to the influence of acute renal failure rat urine amount due to the glycerol
Compare with model group:
*: P＜0.05;
*: P＜0.01
Test of the influence of three Radix Rumicis extracts to acute glomerulonephritis rat urine protein and hematuria
[experiment material]
1. laboratory animal: 100 of SPF level SD rats, male and female half and half, body weight 180～220g.Purchase dimension tonneau China company in Beijing.The raising condition: raise in air-conditioned animal housing, temperature is 20 ℃～25 ℃, relative humidity 60%; 5 in every cage, lighting hours 12 hours is added feedstuff at regular time and quantity; Edible Mus special feed (feed corporation,Ltd of Beijing section Australia association produces) is freely drunk water, and changes bedding and padding every day
2. main agents and medicine:
2.1 reagent:
1. normal saline: the blue pharmaceutcal corporation, Ltd in Tianjin, Tianjin, lot number: 09110904
2. Freund's complete adjuvant: sigma company, CAS:9007-81-2
3. urine protein is measured test kit: bio-engineering research institute, lot number: 20090424 are built up in Nanjing
2.2 medicine:
1. receive the reagent thing: Radix Rumicis extract (extract that the embodiment of the invention 1 prepares)
2. positive drug: dexamethasone; Tianjin Lisheng Pharmaceutical Co., Ltd.; Lot number: 0812026
3. experimental apparatus:
1. metabolic cage: Suzhou City Feng Laboratory Animal Equipment Co., Ltd
2. urine analyzer: the Miditron Junior of Roche Holding Ag II
3. ultraviolet spectrophotometer: Beijing Rayleigh Analytical Instrument Co.,Ltd, UV-1800
[experimental technique]
1. the preparation of renal cortex homogenate:
Use 10% chloral hydrate (0.35ml/100g) intraperitoneal injection of anesthesia animal; The rat that anesthesia is good is cut off both sides hind leg femoral artery and forelimb tremulous pulse with shears, and fully blood-letting is to make things convenient for subsequent operation.Under aseptic condition, open abdomen; The ligation renal artery, renal veins is cut off to the kidney intubate by the self-ligating place; Washing kidney through intubate repeatedly with the normal saline of pre-cooling is white in color; Take off kidney, bark fetching matter grinds to form homogenate with electronic high-speed homogenization machine in ice bath, is sealed in-70 ℃ of refrigerator and cooled after the packing and freezes for use.
2. the foundation of acute glomerulonephritis model:
Get 25 gram cortex homogenate, add Freund's complete adjuvant to 50ml, place mortar to grind well, slowly add normal saline 100ml, the limit edged grinds, and emulsifying is got final product fully.Get emulsifying agent, select for use and get the kidney rat and carry out lumbar injection with the rat of strain, dosage be each 2ml/ only, per two week injections 1 time are injected 7 times altogether, can make rat nephritis model.
3. divide into groups and administration:
After the last injection, measure the urine protein content of each rat respectively, the standard compliant rat of screening urine protein content goes into group.It is 3 groups that qualified rat is divided into, and (positive drug, 2mg/kg) group is irritated stomach every day once, successive administration 14 days to be respectively model group, Radix Rumicis extract group (1000mg/kg) and dexamethasone.
4. draw materials:
Be placed in the metabolic cage in the last administration, collect the twenty-four-hour urine amount,, measure the frequency that hematuria appears in rat urine protein content and each group respectively according to test kit description and instrument operation instruction.
5. statistical method: with experiment one
[experimental result]
1. to the influence of urine protein and hematuria: with model group relatively, positive drug dexamethasone and Radix Rumicis extract all can reduce the content of rat 24h urine protein, and difference has statistical significance (P＜0.01).Compare with model group, Radix Rumicis extract can reduce the occurrence rate of hematuria.Concrete data see table 3 for details
Table 3 Radix Rumicis extract is to the influence of acute glomerulonephritis rat urine protein and hematuria
Compare with model group:
*: P＜0.01
Claims (10)
1. the application of Chinese medicine Radix Rumicis extract in the medicine of preparation treatment nephropathy wherein, contains effective ingredient Radix Rumicis general anthraquinone in the said Radix Rumicis extract, and its mass percentage content in extract is 50-90%.
2. the described application of claim 1 is characterized in that, the extracting mode of said Radix Rumicis is selected from: water is carried, alcohol extraction, reflux extraction, steam distillation, supercritical extraction, decocting method, infusion process, percolation.
3. the described application of claim 1 is characterized in that said extract is obtained through refining with water through alcohol extraction by the Chinese medicine Radix Rumicis.
4. the described application of claim 3 is characterized in that, said alcohol is ethanol.
5. the described application of claim 1 is characterized in that, the method for preparing of said Radix Rumicis extract is following:
(1) fetch earth rhubarb medicinal material or decoction pieces are doubly measured the 60-95% ethanol extraction 2-3 time with 4-8, each 1-3 hour;
(2) after the alcohol extraction medicinal residues are flung to ethanol, extract 2-3 time, each 1-3 hour, merge the water extract with 6-10 times of water gaging;
(3) merge alcohol extract, filter, filtrate to be concentrated into and do not have the alcohol flavor, add an amount of water precipitation, filter, get precipitate A;
(4) supernatant and water extract merge filtration; The polyamide chromatography post of filtrating; The ethanol elution of the 5-20% that water of doubly measuring with resin bed volume 1-5 successively and resin bed volume 2-7 doubly measure is doubly measured the 70-95% ethanol elution with resin bed volume 8-12 at last, collects the 70-95% ethanol elution; Be concentrated into thick extractum, add above-mentioned precipitate A;
(5) an amount of 5-10% sodium hydroxide solution of reuse dissolving filters, and the filtrating adjust pH is to 1-4, and 10 ℃ with held 12-24 hour, filters, and must precipitate drying, promptly gets.
6. application according to claim 1 is characterized in that, the method for preparing of said Radix Rumicis extract is following:
(1) fetch earth rhubarb medicinal material or decoction pieces with 6 times of amount 90% ethanol extractions 2 times, each 2 hours, filter;
(2) after the alcohol extraction medicinal residues are flung to ethanol, extract 2 times, each 1.5 hours, merge the water extract with 8 times of water gagings;
(3) merge alcohol extract, filter, filtrate to be concentrated into and do not have the alcohol flavor, add an amount of water precipitation, filter, get precipitate A,
(4) supernatant and water extract merge to filter, and the polyamide chromatography post of filtrating is used the water of 2.5 times of amounts of resin bed volume and 15% ethanol elution of 5 times of amounts of resin bed volume successively, use 95% ethanol elution of 10 times of amounts of resin bed volume at last; Collect 95% ethanol elution, be concentrated into thick extractum, add above-mentioned precipitate A, an amount of 6% sodium hydroxide solution dissolving of (5) reuse; Filter, filtrating adjust pH to 2.5,10 ℃ with held 12-16 hour; Filter, must precipitate drying, promptly get.
7. application according to claim 1; It is characterized in that said nephropathy comprises: chronic glomerulonephritis, acute glomerulonephritis, latent nephritis, henoch Schonlein purpura nephritis, nephrotic syndrome, diabetic nephropathy, lupus property nephropathy, renal failure, IgA nephropathy or non-IgA nephropathy.
8. Radix Rumicis extract of treating nephropathy, its preparation method is following:
(1) fetch earth rhubarb medicinal material or decoction pieces are doubly measured the 60-95% ethanol extraction 2-3 time with 4-8, each 1-3 hour;
(2) after the alcohol extraction medicinal residues are flung to ethanol, extract 2-3 time, each 1-3 hour, merge the water extract with 6-10 times of water gaging;
(3) merge alcohol extract, filter, filtrate to be concentrated into and do not have the alcohol flavor, add an amount of water precipitation, filter, get precipitate A;
(4) supernatant and water extract merge filtration; The polyamide chromatography post of filtrating; The ethanol elution of the 5-20% that water of doubly measuring with resin bed volume 1-5 successively and resin bed volume 2-7 doubly measure is doubly measured the 70-95% ethanol elution with resin bed volume 8-12 at last, collects the 70-95% ethanol elution; Be concentrated into thick extractum, add above-mentioned precipitate A;
(5) an amount of 5-10% sodium hydroxide solution of reuse dissolving filters, and the filtrating adjust pH is to 1-4, and 10 ℃ with more than held 12-24 hour, filters, and must precipitate drying, promptly gets.
9. pharmaceutical composition that contains the described Radix Rumicis extract of claim 8.
10. pharmaceutical composition according to claim 9 is characterized in that: be made up of active component and pharmacy acceptable auxiliary, said active component Radix Rumicis extract shared percentage by weight in preparation is 0.1-99.9%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104224953A (en) * | 2014-09-28 | 2014-12-24 | 江西百神昌诺药业有限公司 | Total anthraquinones with stable and uniform proportions of various components and composition of total anthraquinones for treating chronic renal failure |
| CN104224951A (en) * | 2014-09-28 | 2014-12-24 | 江西百神药业股份有限公司 | Total anthraquinones with stable and uniform proportions of various components and composition of total anthraquinones for treating cholecystitis |
| CN104224952A (en) * | 2014-09-28 | 2014-12-24 | 江西百神昌诺药业有限公司 | Preparation method for total anthraquinones of rheum officinale with stable and uniform proportions of all components |
-
2011
- 2011-02-15 CN CN201110038081.0A patent/CN102641342B/en not_active Expired - Fee Related
Non-Patent Citations (5)
| Title |
|---|
| 丁力晓: "土大黄研究进展", 《甘肃中医》 * |
| 孙清政: "土大黄治疗小儿急性肾功能衰竭", 《中国乡村医生杂志》 * |
| 王雪芹等: "武当山5种土大黄中3种蒽醌类成分的含量比较", 《医药导报》 * |
| 马燕如等: "大黄酚和大黄酸的合成", 《合成化学》 * |
| 高锦明: "《植物化学》", 30 April 2003, 科学出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104224953A (en) * | 2014-09-28 | 2014-12-24 | 江西百神昌诺药业有限公司 | Total anthraquinones with stable and uniform proportions of various components and composition of total anthraquinones for treating chronic renal failure |
| CN104224951A (en) * | 2014-09-28 | 2014-12-24 | 江西百神药业股份有限公司 | Total anthraquinones with stable and uniform proportions of various components and composition of total anthraquinones for treating cholecystitis |
| CN104224952A (en) * | 2014-09-28 | 2014-12-24 | 江西百神昌诺药业有限公司 | Preparation method for total anthraquinones of rheum officinale with stable and uniform proportions of all components |
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