CN102617702A - Production method of peptidoglycan - Google Patents
Production method of peptidoglycan Download PDFInfo
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- CN102617702A CN102617702A CN2012100620240A CN201210062024A CN102617702A CN 102617702 A CN102617702 A CN 102617702A CN 2012100620240 A CN2012100620240 A CN 2012100620240A CN 201210062024 A CN201210062024 A CN 201210062024A CN 102617702 A CN102617702 A CN 102617702A
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 12
- MSFSPUZXLOGKHJ-UHFFFAOYSA-N Muraminsaeure Natural products OC(=O)C(C)OC1C(N)C(O)OC(CO)C1O MSFSPUZXLOGKHJ-UHFFFAOYSA-N 0.000 title abstract 6
- 108010013639 Peptidoglycan Proteins 0.000 title abstract 6
- 239000007788 liquid Substances 0.000 claims abstract description 43
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 43
- 238000000034 method Methods 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000002994 raw material Substances 0.000 claims abstract description 17
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 15
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 15
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000000909 electrodialysis Methods 0.000 claims abstract description 13
- 229920001542 oligosaccharide Polymers 0.000 claims abstract description 8
- 150000002482 oligosaccharides Chemical class 0.000 claims abstract description 8
- 238000000746 purification Methods 0.000 claims abstract description 7
- 238000001223 reverse osmosis Methods 0.000 claims abstract description 7
- 150000004676 glycans Chemical class 0.000 claims description 19
- 229920001282 polysaccharide Polymers 0.000 claims description 19
- 239000005017 polysaccharide Substances 0.000 claims description 19
- 238000001914 filtration Methods 0.000 claims description 18
- 239000007787 solid Substances 0.000 claims description 18
- 239000000126 substance Substances 0.000 claims description 18
- 239000000463 material Substances 0.000 claims description 17
- 239000000047 product Substances 0.000 claims description 16
- 102000004190 Enzymes Human genes 0.000 claims description 12
- 108090000790 Enzymes Proteins 0.000 claims description 12
- 239000011347 resin Substances 0.000 claims description 12
- 229920005989 resin Polymers 0.000 claims description 12
- 239000002253 acid Substances 0.000 claims description 9
- 238000005406 washing Methods 0.000 claims description 9
- 241000209094 Oryza Species 0.000 claims description 7
- 235000007164 Oryza sativa Nutrition 0.000 claims description 7
- 238000005238 degreasing Methods 0.000 claims description 7
- 235000009566 rice Nutrition 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 6
- 230000002779 inactivation Effects 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 238000004806 packaging method and process Methods 0.000 claims description 6
- 239000001397 quillaja saponaria molina bark Substances 0.000 claims description 6
- 229930182490 saponin Natural products 0.000 claims description 6
- 150000007949 saponins Chemical class 0.000 claims description 6
- 238000005507 spraying Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 239000000758 substrate Substances 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 4
- 239000012141 concentrate Substances 0.000 claims description 3
- 239000000470 constituent Substances 0.000 claims description 3
- 239000000835 fiber Substances 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 235000019658 bitter taste Nutrition 0.000 abstract description 3
- 238000002360 preparation method Methods 0.000 abstract description 3
- 235000019640 taste Nutrition 0.000 abstract 3
- 238000003916 acid precipitation Methods 0.000 abstract 1
- 238000004042 decolorization Methods 0.000 abstract 1
- 235000012054 meals Nutrition 0.000 abstract 1
- 238000001694 spray drying Methods 0.000 abstract 1
- 244000068988 Glycine max Species 0.000 description 4
- 235000010469 Glycine max Nutrition 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 206010010774 Constipation Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to a production method of peptidoglycan, and mainly solves the technical problems that: in the prior art, the production process is complicated, the cost is high, and the peptide tastes bad and is inconvenient to eat. The production method of the peptidoglyca comprises the following steps that: degreased cryogenic meal is used as a raw material to extract peptidoglycan; and protein isolate and oligosaccharide can also be used as raw materials to extract the peptidoglycan. The weight ratio of feed to water is 1:15, the pH value of a feed liquid is 9-11, and the raw materials are fully stirred for more than 5 hours at a temperature of 50 DEG C to 55 DEG C, are subjected to acid precipitation and water wash to purify protein, are separated by a decanter, and are subjected to electrodialysis, decolorization column purification, reverse osmosis concentration, activated carbon treatment and final spray drying to obtain a peptidoglycan product. The production method of the peptidoglycan has the characteristics that the preparation method is simple, the production cost of the product is low, the product is convenient to eat, has no bitterness, and tastes good. The problem that the peptide tastes unpleasant is solved, and the technical problem that the efficacy of peptide and oligosaccharide products embodies in one product is solved. The method has a good promotion prospect.
Description
Technical field
The present invention relates to a kind of Polysaccharides, peptide complexes working method, exactly is a kind of with peptide and oligose polymeric novel method, belongs to the soybean deep processing technology field.
Background technology
In recent years, the technology of soybean deep processing is constantly improving, and the peptide that wherein in soybean, prepares has the raising body immunity, strengthens physical efficiency, and is obvious to the human body cell repairing effect, is a kind of true solution, absorbs easily.Though the nutritive ingredient of peptide is generally acknowledged that by people because peptide has bitter taste, its mouthfeel is poor, the promotion and application of peptide have therefore been influenced.Oligose is the extract in the soybean, has the two-way effect of conditioning diarrhoea and constipation, also can improve looks.People when edible, need respectively that peptide is edible, pretty troublesome, inconvenient with the oligose bath, and the complex manufacturing of product, cost high, produce the sewage pollution environment that discharges.
Summary of the invention
The objective of the invention is the deficiency to above-mentioned prior art, a kind of preparation method is simple, production cost is low and provide, instant, and the Polysaccharides, peptide complexes working method that after peptide and the oligose polymerization nutritive ingredient is enhanced.
The present invention can be a raw material with the degreasing low temperature dregs of rice, extracts Polysaccharides, peptide complexes; Can be that raw material extracts Polysaccharides, peptide complexes also with protein isolates and oligose.
One, be raw material with the degreasing low temperature dregs of rice, the working method of extracting Polysaccharides, peptide complexes realizes through following process step:
A, be raw material with the degreasing low temperature dregs of rice; Material water is by weight being 1: 15, and material liquid pH value 9-11 fully stirs more than 5 hours under 50 ℃ of-55 ℃ of conditions of temperature; Effective constituent is fully leached; The feed liquid that leaches is through the horizontal helical type separating machine, and rotating speed is in the zone of 3500-4500r/min, with mixing solutions such as water soluble oligosaccharide, albumen, NOVASOY 400 and saponin and undissolved robust fibre separately.
B, the heavy albumen of acid: the water-soluble mixed solution acid after the step a separation is heavy; Adjust pH 4.45 ± 0.2; Separate through the horizontal helical type separating machine, rotating speed separates albumen and mixing solutionss such as water soluble oligosaccharide, saponin, NOVASOY 400 in the zone of 3500-4500r/min.
C, washing albumen: material water is by weight 1: 70, material liquid pH value 9.5-11, and 50 ℃-55 ℃ of temperature are washed and albumen were all dissolved in 2 hours, recall to pH value 4.45 ± 0.2 more again, separate with the horizontal helical type separating machine once more.
D, enzymolysis process: what enzyme used is the Sumizyme MP that Wuxi snow Mei Zhijichang produces; Concentration of substrate 3.5%, 55 ℃-60 ℃ of temperature, pH value 7.5-11; Enzyme concentration is the 0.5-2.0% of albumen solubility rate; Enzymolysis after inactivation finishes, is purified through Plate Filtration, electrodialysis, resin column more than 6 hours successively.
E, with the water-soluble mixed solution after the heavy albumen sepn of acid among the step b, be made into the 1.5%-1.8% solid substance, pH value 3.0 is successively through Plate Filtration, electrodialysis, resin column purification.
Feed liquid after f, the purification is respectively through reverse osmosis concentration, peptide liquid solid substance 8%-10%, pH value 4.1-4.4, oligose liquid solid substance 8%-10%, pH value 4.1-4.4.
G, peptide liquid concentration are puddled together in the 40%-20% ratio at 60%-80% and oligomeric sugar concentration, use activated carbon decolorizing, 70 ℃ of temperature; The gac addition is the 1.5%-2.0% of feed liquid weight; Stirred 1 hour, through Plate Filtration, the direct spraying drying of filtrating; Promptly get the Polysaccharides, peptide complexes product, with said product sterilising packaging.
Two, be raw material with protein isolates and oligose, the working method of extracting Polysaccharides, peptide complexes realizes through following process step:
A, washing protein isolates: material water is by weight 1: 70, material liquid pH value 9.5-11, and 50 ℃-55 ℃ of temperature make albumen all after the dissolving, and adjust pH 4.45 ± 0.2 separates through the horizontal helical type separating machine, and rotating speed is in the zone of 3500-4500r/min.
B, enzymolysis process: what enzyme used is the Sumizyme MP that Wuxi snow Mei Zhijichang produces; It is 3.5% that albumen after the washing is made into concentration of substrate, 55 ℃-60 ℃ of temperature, pH value 7.5-11; Enzyme concentration is the 0.5-2.0% of protein isolates weight; Enzymolysis after inactivation finishes, is purified through Plate Filtration, electrodialysis, resin column more than 6 hours successively.
The supernatant of c, production protein concentrate is as the raw material of oligose, and solid substance is made into 1.5-2.0%, and pH value 3.0 is purified through Plate Filtration, electrodialysis, resin column successively.
D, the feed liquid after will purifying are respectively through reverse osmosis concentration, peptide liquid solid substance 8%-10%, pH value 4.1-4.4, oligose liquid solid substance 8%-10%, pH value 4.1-4.4.
E, peptide liquid concentration are puddled together in the 40%-20% ratio at 60%-80% and oligomeric sugar concentration, use activated carbon decolorizing, 70 ℃ of temperature; The gac addition is the 1.5%-2.0% of feed liquid weight; Stirred 1 hour, through Plate Filtration, the direct spraying drying of filtrating; Promptly get the Polysaccharides, peptide complexes product, with said product sterilising packaging.
Characteristics of the present invention are: the preparation method is simple, the products production cost is low, instant, and no bitter taste, mouthfeel are good.When having solved the unhappy problem of peptide mouthfeel, solved two kinds of product efficacy of peptide and oligose again and be embodied in the technical problem on a kind of product.Present method has the excellent popularization prospect.
Embodiment
Embodiment one
With the degreasing low temperature dregs of rice is raw material, and the working method of extracting Polysaccharides, peptide complexes realizes through following process step:
A, be raw material with the degreasing low temperature dregs of rice; Material water is by weight being 1: 15, and material liquid pH value 9-11 fully stirs more than 5 hours under 50 ℃ of-55 ℃ of conditions of temperature; Effective constituent is fully leached; The feed liquid that leaches is through the horizontal helical type separating machine, and rotating speed is in the zone of 3500-4500r/min, with mixing solutions such as water soluble oligosaccharide, albumen, NOVASOY 400 and saponin and undissolved robust fibre separately.
B, the heavy albumen of acid: the depth of water property mixed solution acid after the step a separation is heavy; Adjust pH 4.45 ± 0.2 separates through the horizontal helical type separating machine; Rotating speed is in the zone of 3500-4500r/min, with mixing solutionss such as albumen and water soluble oligosaccharide, saponin, NOVASOY 400 separately.
C, washing albumen: expect water by weight 1: 70, material liquid pH value 9.5-11, temperature is washed till 2 hours for 50 ℃-55 ℃ all dissolves albumen, recalls to pH value 4.45 ± 0.2 more again, separates with the horizontal helical type separating machine once more.
D, enzymolysis process: what enzyme used is the Sumizyme MP that Wuxi snow Mei Zhijichang produces; Concentration of substrate 3.5%, 55 ℃-60 ℃ of temperature, pH value 7.5-11; Enzyme concentration is the 0.5-2.0% of albumen solubility rate; Enzymolysis after inactivation finishes, is purified through Plate Filtration, electrodialysis, resin column more than 6 hours successively.
E, with the water-soluble mixed solution after the heavy albumen sepn of acid among the step b, be made into the 1.5%-1.8% solid substance, pH value 3.0 is successively through Plate Filtration, electrodialysis, resin column purification.
Feed liquid after f, the purification is respectively through reverse osmosis concentration, peptide liquid solid substance 8%-10%, pH value 4.1-4.4, oligose liquid solid substance 8%-10%, pH value 4.1-4.4.
G, peptide liquid concentration are puddled together in the 40%-20% ratio at 60%-80% and oligomeric sugar concentration, use activated carbon decolorizing, 70 ℃ of temperature; The gac addition is the 1.5%-2.0% of feed liquid weight; Stirred 1 hour, through Plate Filtration, the direct spraying drying of filtrating; Promptly get the Polysaccharides, peptide complexes product, with said product sterilising packaging.
Embodiment two
With protein isolates and oligose is the working method that raw material extracts Polysaccharides, peptide complexes:
A, washing protein isolates: material water is by weight 1: 70, material liquid pH value 9.5-11, and 50 ℃-55 ℃ of temperature make albumen all after the dissolving, and adjust pH 4.45 ± 0.2 separates through the horizontal helical type separating machine, and rotating speed is in the zone of 3500-4500r/min.
B, enzymolysis process: what enzyme used is the Sumizyme MP that Wuxi snow Mei Zhijichang produces; It is 3.5% that albumen after the washing is made into concentration of substrate, 55 ℃-60 ℃ of temperature, pH value 7.5-11; Enzyme concentration is the 0.5-2.0% of protein isolates weight; Enzymolysis after inactivation finishes, is purified through Plate Filtration, electrodialysis, resin column more than 6 hours successively.
The supernatant of c, production protein concentrate is as the raw material of oligose, and solid substance is made into 1.5-2.0%, and pH value 3.0 is purified through Plate Filtration, electrodialysis, resin column successively.
D, the feed liquid after will purifying are respectively through reverse osmosis concentration, peptide liquid solid substance 8%-10%, pH value 4.1-4.4, oligose liquid solid substance 8%-10%, pH value 4.1-4.4.
E, peptide liquid concentration are puddled together in the 40%-20% ratio at 60%-80% and oligomeric sugar concentration, use activated carbon decolorizing, 70 ℃ of temperature; The gac addition is the 1.5%-2.0% of feed liquid weight; Stirred 1 hour, through Plate Filtration, the direct spraying drying of filtrating; Promptly get the Polysaccharides, peptide complexes product, with said product sterilising packaging.
Claims (2)
1. Polysaccharides, peptide complexes working method, this method is a raw material with the degreasing low temperature dregs of rice, extracts Polysaccharides, peptide complexes, concrete steps are described below:
A, be raw material with the degreasing low temperature dregs of rice; Material water is by weight being 1: 15, and material liquid pH value 9-11 fully stirs more than 5 hours under 50 ℃ of-55 ℃ of conditions of temperature; Effective constituent is fully leached; The feed liquid that leaches is through the horizontal helical type separating machine, and rotating speed is in the zone of 3500-4500r/min, with mixing solutions such as water soluble oligosaccharide, albumen, NOVASOY 400 and saponin and undissolved robust fibre separately;
B, the heavy albumen of acid: the water-soluble mixed solution acid after the step a separation is heavy; Adjust pH 4.45 ± 0.2; Separate through the horizontal helical type separating machine, rotating speed separates albumen and mixing solutionss such as water soluble oligosaccharide, saponin, NOVASOY 400 in the zone of 3500-4500r/min;
C, washing albumen: material water is by weight 1: 70, material liquid pH value 9.5-11, and 50 ℃-55 ℃ of temperature are washed and albumen were all dissolved in 2 hours, recall to pH value 4.45 ± 0.2 more again, separate with the horizontal helical type separating machine once more;
D, enzymolysis process: what enzyme used is the Sumizyme MP that Wuxi snow Mei Zhijichang produces; Concentration of substrate 3.5%, 55 ℃-60 ℃ of temperature, pH value 7.5-11; Enzyme concentration is the 0.5-2.0% of albumen solubility rate; Enzymolysis after inactivation finishes, is purified through Plate Filtration, electrodialysis, resin column more than 6 hours successively;
E, with the water-soluble mixed solution after the heavy albumen sepn of acid among the step b, be made into the 1.5%-1.8% solid substance, pH value 3.0 is successively through Plate Filtration, electrodialysis, resin column purification;
Feed liquid after f, the purification is respectively through reverse osmosis concentration, peptide liquid solid substance 8%-10%, pH value 4.1-4.4, oligose liquid solid substance 8%-10%, pH value 4.1-4.4;
G, peptide liquid concentration are puddled together in the 40%-20% ratio at 60%-80% and oligomeric sugar concentration, use activated carbon decolorizing, 70 ℃ of temperature; The gac addition is the 1.5%-2.0% of feed liquid weight; Stirred 1 hour, through Plate Filtration, the direct spraying drying of filtrating; Promptly get the Polysaccharides, peptide complexes product, with said product sterilising packaging.
2. Polysaccharides, peptide complexes working method, this method is a raw material with protein isolates and oligose, extracts Polysaccharides, peptide complexes, concrete steps are described below:
A, washing protein isolates: material water is by weight 1: 70, material liquid pH value 9.5-11, and 50 ℃-55 ℃ of temperature make albumen all after the dissolving, and adjust pH 4.45 ± 0.2 separates through the horizontal helical type separating machine, and rotating speed is in the zone of 3500-4500r/min;
B, enzymolysis process: what enzyme used is the Sumizyme MP that Wuxi snow Mei Zhijichang produces; It is 3.5% that albumen after the washing is made into concentration of substrate, 55 ℃-60 ℃ of temperature, pH value 7.5-11; Enzyme concentration is the 0.5-2.0% of protein isolates weight; Enzymolysis after inactivation finishes, is purified through Plate Filtration, electrodialysis, resin column more than 6 hours successively;
The supernatant of c, production protein concentrate is as the raw material of oligose, and solid substance is made into 1.5-2.0%, and pH value 3.0 is purified through Plate Filtration, electrodialysis, resin column successively;
D, the feed liquid after will purifying are respectively through reverse osmosis concentration, peptide liquid solid substance 8%-10%, pH value 4.1-4.4, oligose liquid solid substance 8%-10%, pH value 4.1-4.4;
E, peptide liquid concentration are puddled together in the 40%-20% ratio at 60%-80% and oligomeric sugar concentration, use activated carbon decolorizing, 70 ℃ of temperature; The gac addition is the 1.5%-2.0% of feed liquid weight; Stirred 1 hour, through Plate Filtration, the direct spraying drying of filtrating; Promptly get the Polysaccharides, peptide complexes product, with said product sterilising packaging.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100620240A CN102617702A (en) | 2012-03-09 | 2012-03-09 | Production method of peptidoglycan |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100620240A CN102617702A (en) | 2012-03-09 | 2012-03-09 | Production method of peptidoglycan |
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| Publication Number | Publication Date |
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| CN102617702A true CN102617702A (en) | 2012-08-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012100620240A Pending CN102617702A (en) | 2012-03-09 | 2012-03-09 | Production method of peptidoglycan |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103113481A (en) * | 2012-08-16 | 2013-05-22 | 中南民族大学 | Mutant strain ZN0871v11 cell wall peptidoglycan and preparation method thereof |
| CN105907824A (en) * | 2016-05-10 | 2016-08-31 | 江苏巨托生物科技有限公司 | Method for producing glycopeptide by taking edible soybean meal as raw material |
| CN109619264A (en) * | 2018-10-29 | 2019-04-16 | 长春大学 | The clean preparation method of the compound water-soluble function factor of soybean probiotic peptide |
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| JPH04183371A (en) * | 1990-11-15 | 1992-06-30 | Snow Brand Milk Prod Co Ltd | Bone-fortifying food, feed and pharmaceutical |
| CN1282525A (en) * | 1999-08-03 | 2001-02-07 | 姜浩奎 | Process for extracting soybean protein |
| CN1323534A (en) * | 2000-05-15 | 2001-11-28 | 姜浩奎 | Continuous technolgical process of extracting soybean and separating protein, isoflavone, oligosaccharide and saponin |
| CN1327983A (en) * | 2000-06-08 | 2001-12-26 | 姜浩奎 | Process for continuously extracting glycitin, olegose and saponin |
| CN1329103A (en) * | 2001-08-07 | 2002-01-02 | 姜浩奎 | Method for extracting protein, short peptide, nucleic acid, isoflavone, saponin and oligosaccharide by using high and low temperature soybean cake |
| CN1425323A (en) * | 2003-01-09 | 2003-06-25 | 姜浩奎 | Method for extracting composite soy bean function factor from high and low temperature degreased bean dregs |
| CN1488274A (en) * | 2002-10-10 | 2004-04-14 | 吉林省高等院校科技开发研究中心 | Method for producing soybean protein which content is not less than 60% useing high-low temperature bean dregs as raw material |
| CN1522596A (en) * | 2003-02-17 | 2004-08-25 | 吉林省高等院校科技开发研究中心 | Method for extracting composite corn embryo health care functional factor having corn embryo cake as raw material |
| CN1537865A (en) * | 2003-10-23 | 2004-10-20 | 姜浩奎 | Method of extracting lactoalbumin, nucleic acid oligosaccharide, isoflavone, saponin from yellow waste water generated by producing soybean |
-
2012
- 2012-03-09 CN CN2012100620240A patent/CN102617702A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04183371A (en) * | 1990-11-15 | 1992-06-30 | Snow Brand Milk Prod Co Ltd | Bone-fortifying food, feed and pharmaceutical |
| CN1282525A (en) * | 1999-08-03 | 2001-02-07 | 姜浩奎 | Process for extracting soybean protein |
| CN1323534A (en) * | 2000-05-15 | 2001-11-28 | 姜浩奎 | Continuous technolgical process of extracting soybean and separating protein, isoflavone, oligosaccharide and saponin |
| CN1327983A (en) * | 2000-06-08 | 2001-12-26 | 姜浩奎 | Process for continuously extracting glycitin, olegose and saponin |
| CN1329103A (en) * | 2001-08-07 | 2002-01-02 | 姜浩奎 | Method for extracting protein, short peptide, nucleic acid, isoflavone, saponin and oligosaccharide by using high and low temperature soybean cake |
| CN1488274A (en) * | 2002-10-10 | 2004-04-14 | 吉林省高等院校科技开发研究中心 | Method for producing soybean protein which content is not less than 60% useing high-low temperature bean dregs as raw material |
| CN1425323A (en) * | 2003-01-09 | 2003-06-25 | 姜浩奎 | Method for extracting composite soy bean function factor from high and low temperature degreased bean dregs |
| CN1522596A (en) * | 2003-02-17 | 2004-08-25 | 吉林省高等院校科技开发研究中心 | Method for extracting composite corn embryo health care functional factor having corn embryo cake as raw material |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103113481A (en) * | 2012-08-16 | 2013-05-22 | 中南民族大学 | Mutant strain ZN0871v11 cell wall peptidoglycan and preparation method thereof |
| CN103113481B (en) * | 2012-08-16 | 2014-12-10 | 中南民族大学 | Mutant strain ZN0871v11 cell wall peptidoglycan and preparation method thereof |
| CN105907824A (en) * | 2016-05-10 | 2016-08-31 | 江苏巨托生物科技有限公司 | Method for producing glycopeptide by taking edible soybean meal as raw material |
| CN105907824B (en) * | 2016-05-10 | 2022-03-29 | 江苏巨托生物科技有限公司 | Method for producing glycopeptide by taking edible soybean meal as raw material |
| CN109619264A (en) * | 2018-10-29 | 2019-04-16 | 长春大学 | The clean preparation method of the compound water-soluble function factor of soybean probiotic peptide |
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