CN102608336A - Disposable transfusion and cross matching experiment combiner - Google Patents

Abstract

The invention relates to medical experiment equipment, which is a disposable blood transfusion cross-matching experimental combiner, which has a small transparent plastic test tube, and two sample tubes are connected to the upper opening of the small test tube at the same time, and the small material is passed through the upper part of the small test tube. There is a transparent plastic film bag in the cross-connection of the tube; the present invention solves the problem that the existing blood transfusion cross-matching experiment has no special equipment, and the operation using ordinary test tubes is very complicated and cumbersome, and it is easy to make mistakes. The present invention is mainly used in blood transfusion departments of various hospitals. The central blood station, clinical laboratory, etc. conduct cross-matching experiments for blood transfusion patients.

Description

Disposable blood transfusion cross-matching experiment combiner

(1) Technical field: the present invention relates to medical experiment equipment, especially a kind of disposable blood transfusion cross-matching experimental combiner. the

(2) Background technology: So far, blood transfusion departments of hospitals at all levels have done blood transfusion cross-matching experiments based on the principle of safe blood transfusion, applying for blood sources with the same type of ABO blood type and RH blood type (such as type A RH positive patients need blood transfusions) Then you must seek a type A RH-positive blood donor), separate the blood cells and serum of the blood transfusion recipient and the blood recipient, cross each other and let them react in the test tube, then observe the hemolysis and agglutination phenomenon, and judge whether the safe blood transfusion can be implemented, that is, Perform a cross-matching experiment. In the prior art, there is no special equipment for blood transfusion cross-matching experiments, and ordinary test tubes are usually used for experiments. If a patient only transfused blood for one person, they should take the primary and secondary test tubes and add them respectively. : Recipient's serum + blood donor's blood cells (main tube): Recipient's blood cells + blood donor's serum (secondary tube). Centrifuge at 25-30°C for 5-10 minutes and take it out to observe with the naked eye whether there is hemolysis and agglutination, then drop the blood after the fork into a glass slide and observe whether there is agglutination with a microscope, and finally report whether there is hemolysis and agglutination in the primary and secondary tubes, The conclusion is "consistent" or "inconsistent" to judge whether the blood of this blood donor can be transfused. the

If the blood recipient needs a large number of blood transfusions (more than two parts) of the donor's blood, the cross-matching test before the blood transfusion is more complicated. For example, two-fork one needs six test tubes (because cross-matching experiments are performed between each blood donor and blood recipient and between each blood donor), and if the blood recipient needs three-fork one, twelve test tubes are needed. Repeatedly add blood cells and serum between blood recipients from numbering to sample addition, and repeatedly add samples at intervals (only for the task of adding samples, the operator uses the sampler to add blood cells and serum to each test tube once, then Twelve test tubes need to be sampled 24 times, and blood cells and serum should be added to the main tube and the secondary tube in a paired and misplaced manner, which shows that the operation is very complicated and cumbersome), so multiple blood suppliers are required. complex and error-prone. the

(3) Contents of the invention: The purpose of the present invention is to solve the problem that there is no special equipment for the existing blood transfusion cross-matching experiment, and the experiment is carried out with ordinary test tubes, the operation is very complicated and cumbersome, and it is easy to make mistakes. It provides a one-time blood transfusion cross-matching Blood test combiner. the

The specific scheme of the present invention is: a small transparent plastic test tube is designed, and it is characterized in that: two sample feeding tubes are docked at the upper opening of the small test tube at the same time, and a transparent plastic film is cross-connected on the upper part of the small test tube through a small feeding tube bag. the

In the present invention, the two sample adding tubes and the small test tube are integrally formed or connected by thread structure or superglue. Among them, the use of threaded structure butt joints or superglue butt joints has better manufacturability. the

In order to conveniently and quickly distinguish the two sample tubes during operation, the two sample tubes in the present invention are designed as one long and one short. the

It is also possible to design the cross-sectional shapes of the heads of the two sample feeding tubes into two different shapes. the

From the perspective of manufacturing technology, one of the head cross-sectional shapes of the two sample feeding tubes described in the present invention is circular and the other is square; or one is oval and the other is square; or one is One is round and the other is rectangular. the

The present invention has simple structure, low manufacturing cost, one-time use, safety and reliability, and the application of the present invention to carry out blood transfusion cross-matching experiment is convenient, fast and less prone to error; and because the present invention is designed with a transparent plastic film capsule, it can be directly performed by microscope Observe each cross-reaction, whether there are adverse reactions such as small agglutination, which saves the round-trip sampling time for each glass test tube to slide or the possibility of infection. the

(4) Description of the drawings:

Fig. 1 is a structural representation of the present invention;

Fig. 2 is a combined use state diagram of carrying out one-fork-one experiment by adopting the present invention;

Fig. 3 is a combined use state diagram of the binary-one experiment carried out by the present invention.

Among the figure: 1-sample feeding tube, 2-sample feeding tube, 3-film capsule, 4-small feed tube, 5-small test tube. the

(5) Specific implementation methods:

Referring to Fig. 1, the present invention adopts the small test tube 5 of transparent plastic molding, and two sample tubes 1 and 2 are docked on the small test tube 5, wherein the head section of sample tube 1 is square, and the head section of sample tube 2 is square. The cross-section of the top is circular to show the difference; the heads of the two sample pipes 1 and 2 can also be made into two different cross-sectional shapes, such as circle and rectangle, rectangle and square, ellipse and square, Various combinations such as hexagons and rectangles. It is also possible to make the two sampling tubes one long and one short to show the difference. Of course, it is also feasible to make the two sample tubes have the same cross-section, and the same length. It is enough to be careful during operation, or it is also a better method to make different marks on the sample tubes. The top of the medium and small test tube 5 of the present invention is also designed and formed with a small feed tube 4 leading to the outside, and a small plastic transparent film capsule 3 is bonded to the small feed tube 4 outer ends, which constitutes the present invention.

The two sample tubes 1 and 2 in the present invention can be molded together with the small test tube 5 at one time, and can also be butted with a thread structure after being molded separately, or joined together with superglue. the

The instructions for use of the present invention are as follows:

1. One-fork-one experiment (when the recipient only needs blood from one donor):

Take two disposable blood transfusion cross-matching experimental combiners, arrange the four sample tubes of the two combiners in two groups according to Figure 2, and combine them in pairs with rubber bands. Blood cell suspension tube, the two square-head sample tubes are serum tubes; one of the small test tubes is the main tube, and the other small test tube is the secondary tube; then add the recipient's serum and blood cell suspension into the primary tube, and add the blood cell suspension in the secondary tube Add blood recipient's blood cell suspension and blood donor serum. Then do the following:

(1) Mix the above two plastic tubes and place them in an incubator at 25°C for 5-10 minutes;

(2) Take out and centrifuge at low speed for 2 minutes, take out and observe the supernatant, whether there is hemolysis or agglutination;

(3) If there is no hemolysis and no agglutination, shake the plastic tube reactant and gently push it into the reaction capsule. Observe with a microscope to see if there is any fine agglutination;

(4) There is no hemolysis or agglutination in the above, and the conclusion is reported as "conforming"; otherwise, a report of "not conforming" will be issued.

2. Binary one experiment (when the recipient needs blood from two donors):

Take 9 disposable blood transfusion cross-matching experimental combiners, arrange the 18 sample tubes of the 9 combiners in six groups according to Figure 3, and group them with rubber bands. And do A, B, C and A', B', C' numbers. Then pour the recipient's blood cell suspension into the three sample tubes of A, pour the first donor's blood cell suspension into the three sample tubes of B, and pour the second donor's blood cells into the three sample tubes of C Then pour the recipient's blood into the three sample tubes of A', pour the serum of the first blood donor into the three tubes of B', and pour the serum of the second donor into the three tubes of C'. Blood donor serum. Subsequent operations and reports are the same as for an experiment.

the

Claims (5)

1. Disposable blood transfusion cross-matching experimental combiner, with a small transparent plastic test tube, characterized in that two sample tubes are connected to the upper opening of the small test tube at the same time, and a small feeding tube is cross-connected to the upper part of the small test tube. Transparent plastic film pouch. 2. The disposable blood transfusion cross-matching experimental combiner according to claim 1, characterized in that: the two sample tubes and the small test tube are integrally formed or connected by a screw thread structure or connected by superglue. 3. The disposable blood transfusion cross-matching experimental combiner according to claim 1 or 2, characterized in that: the two sample feeding tubes are designed as one long and one short. 4. The disposable blood transfusion cross-matching experiment combiner according to claim 1 or 2, characterized in that: the cross-sectional shapes of the heads of the two sample feeding tubes are two different shapes. 5. The disposable blood transfusion cross-matching experimental combiner according to claim 4, characterized in that: one of the cross-sectional shapes of the heads of the two sample feeding tubes is a circle, and the other is a square; or a One is oval and the other is square; or one is round and the other is rectangular.

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