CN102578361B - Maggot compound protein powder - Google Patents
Maggot compound protein powder Download PDFInfo
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- CN102578361B CN102578361B CN 201210084977 CN201210084977A CN102578361B CN 102578361 B CN102578361 B CN 102578361B CN 201210084977 CN201210084977 CN 201210084977 CN 201210084977 A CN201210084977 A CN 201210084977A CN 102578361 B CN102578361 B CN 102578361B
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- Prior art keywords
- maggot
- protein powder
- powder
- slurry
- compound protein
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- 239000000843 powder Substances 0.000 title claims abstract description 43
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 31
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 31
- 150000001875 compounds Chemical class 0.000 title claims abstract description 21
- 102000004190 Enzymes Human genes 0.000 claims abstract description 14
- 108090000790 Enzymes Proteins 0.000 claims abstract description 14
- 229920001184 polypeptide Polymers 0.000 claims abstract description 10
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 10
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 10
- 239000013049 sediment Substances 0.000 claims abstract description 10
- 239000006228 supernatant Substances 0.000 claims abstract description 10
- 238000004108 freeze drying Methods 0.000 claims abstract description 5
- 238000001694 spray drying Methods 0.000 claims abstract description 5
- 230000001954 sterilising effect Effects 0.000 claims abstract description 5
- 239000002002 slurry Substances 0.000 claims description 20
- 229940088598 enzyme Drugs 0.000 claims description 12
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- 238000001976 enzyme digestion Methods 0.000 claims description 8
- 230000001105 regulatory effect Effects 0.000 claims description 8
- 102000057297 Pepsin A Human genes 0.000 claims description 6
- 108090000284 Pepsin A Proteins 0.000 claims description 6
- 229940111202 pepsin Drugs 0.000 claims description 6
- 239000012141 concentrate Substances 0.000 claims description 4
- 230000009849 deactivation Effects 0.000 claims description 4
- 238000010298 pulverizing process Methods 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 239000002253 acid Substances 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 abstract description 2
- 230000007774 longterm Effects 0.000 abstract description 2
- 238000004140 cleaning Methods 0.000 abstract 2
- 230000000415 inactivating effect Effects 0.000 abstract 2
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 239000000796 flavoring agent Substances 0.000 description 5
- 235000019634 flavors Nutrition 0.000 description 5
- 108091005508 Acid proteases Proteins 0.000 description 4
- 108091005804 Peptidases Proteins 0.000 description 4
- 239000004365 Protease Substances 0.000 description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 4
- 239000003513 alkali Substances 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 238000005273 aeration Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000002318 cardia Anatomy 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000643 oven drying Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 229940116540 protein supplement Drugs 0.000 description 1
- 235000005974 protein supplement Nutrition 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses maggot compound protein powder, which is made through the steps of: cleaning alive maggots, draining, grinding into thick liquid, sterilizing, inactivating enzymes, conducting enzymolysis in acid, inactivating enzymes, conducting enzymolysis in alkaline, centrifuging and respectively collecting supernatant and sediment; concentrating and spray drying the supernatant to obtain maggot polypeptide powder; cleaning, freeze drying, crushing and screening the sediment to obtain maggot protein powder; and mixing the maggot polypeptide powder with the maggot protein powder to obtain the maggot compound protein powder. The maggot compound protein powder disclosed by the invention can reserve all nutrients of the maggots, is not smelly and can be easily preserved for a long term.
Description
A kind of maggot compound protein powder.
Technical field
The present invention relates to a kind of compound protein powder, be specifically related to a kind of maggot compound protein powder.
Background technology
Edible and the medical value of maggot is more and more paid attention to by people, will become the indispensable senior tonic of people soon.Traditional maggot dry powder is processed with method of making herbs dry gradually in the shade (aeration drying), sunlight be exposed to the sun method, high-temperature oven drying method, and the maggot dry powder that these methods are handled not only nutrition loses in a large number, and the flavor stink smell is smelly, is difficult to eat.And the hydrolyzate of maggot the most effective be enzymatic isolation method, percent hydrolysis can reach about 80%, stink smell is smelly but its liquid is also distinguished the flavor of, and is not easy to store, is difficult to do edible raw material.
For overcoming the above-mentioned defective of prior art, the present invention aims to provide a kind of new maggot compound protein powder preparation method, the maggot compound protein powder that adopts this method to make, and not only nutrition is not lost, and does not have stench flavor, and is easy to deposit, and reaches food sanitation standard fully.
Summary of the invention
The technical problem that the present invention mainly solves provides a kind of maggot compound protein powder, can keep all nutritional labelings of maggot, does not have stench flavor again, and is easy to deposit.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is: a kind of maggot compound protein powder is provided, is prepared as follows:
(1) fresh and alive maggot is cleaned, drained, add 2.5～3.5 times of water, wear into the maggot slurry, 90-95 ℃ of sterilization 10-12min makes the various enzyme deactivations in the maggot simultaneously;
(2) regulating maggot slurry pH4.0-4.5, is 1:350-500 adding acid protease by acid protease with maggot slurry mass ratio, carries out enzyme digestion reaction 2.5-3 hour at 35-40 ℃, and enzyme 6～8 minutes goes out under 90～95 ℃; Then, regulating maggot slurry pH8.0-8.5, is 1:350-500 adding alkali protease by alkali protease and maggot slurry mass ratio, carries out enzyme digestion reaction 1～1.5h in 50～55 ℃, and enzyme 6～8 minutes goes out under 90～95 ℃; Then at 4 ℃, refrigerated centrifuge 10-15min under the 4200g-4500g condition collects supernatant and sediment respectively;
(3) supernatant with step (2) gained concentrate, spray-drying, obtain the maggot polypeptide powder;
(4) sediment to step (2) gained clean, freeze drying, pulverization process, cross the 80-90 mesh sieve, obtain the maggot protein powder;
(5) maggot polypeptide powder and maggot protein powder are mixed, namely get the maggot compound protein powder.
Described acid protease is stomach cardia acid.
Described alkali protease is trypsase.
Described cryodesiccated temperature is subzero 22 ℃-subzero 28 ℃, and the time is 15-20h.
The invention has the beneficial effects as follows:
1. maggot compound protein powder of the present invention can keep all nutritional labelings of maggot, does not have stench flavor again, and is easy to long-term storage.
2. the present invention adopts acid protease and alkali protease, under temperate condition, and the substep enzymolysis, the maggot compound protein powder of acquisition more is conducive to the income of human body, can be used as protein supplements, is widely used in other food.
The specific embodiment
Below preferred embodiment of the present invention is described in detail, thereby so that advantages and features of the invention can be easier to be it will be appreciated by those skilled in the art that protection scope of the present invention is made more explicit defining.
The embodiment of the invention comprises:
Embodiment 1
A kind of maggot compound protein powder is prepared as follows:
(1) fresh and alive maggot is cleaned, drained, add 2.5 times of water, wear into the maggot slurry, 95 ℃ of sterilization 10min make the various enzyme deactivations in the maggot simultaneously;
(2) regulating maggot slurry pH4.0, is 1:350 adding pepsin by pepsin with maggot slurry mass ratio, carries out enzyme digestion reaction 2.5 hours at 40 ℃, and enzyme 8 minutes goes out under 95 ℃; Then, regulating maggot slurry pH8.0, is 1:350 adding trypsase by trypsase and maggot slurry mass ratio, carries out enzyme digestion reaction 1.5h in 55 ℃, and enzyme 8 minutes goes out under 95 ℃; Then at 4 ℃, refrigerated centrifuge 10min under the 4500g condition collects supernatant and sediment respectively;
(3) supernatant with step (2) gained concentrate, spray-drying, obtain the maggot polypeptide powder;
(4) sediment to step (2) gained clean, subzero 22 ℃ of freeze drying 20h, pulverization process, cross 80 mesh sieves, obtain the maggot protein powder;
(5) maggot polypeptide powder and maggot protein powder are mixed, namely get the maggot compound protein powder.
Embodiment 2
A kind of maggot compound protein powder is prepared as follows:
(1) fresh and alive maggot is cleaned, drained, add 3.5 times of water, wear into the maggot slurry, 90 ℃ of sterilization 12min make the various enzyme deactivations in the maggot simultaneously;
(2) regulating maggot slurry pH4.5, is 1:500 adding pepsin by pepsin with maggot slurry mass ratio, carries out enzyme digestion reaction 3 hours at 35 ℃, and enzyme 8 minutes goes out under 90 ℃; Then, regulating maggot slurry pH8.5, is 1:500 adding trypsase by trypsase and maggot slurry mass ratio, carries out enzyme digestion reaction 1h in 50 ℃, and enzyme 8 minutes goes out under 90 ℃; Then at 4 ℃, refrigerated centrifuge 10-15min under the 4200g condition collects supernatant and sediment respectively;
(3) supernatant with step (2) gained concentrate, spray-drying, obtain the maggot polypeptide powder;
(4) sediment to step (2) gained clean, subzero 28 ℃ of freeze drying 15h, pulverization process, cross 90 mesh sieves, obtain the maggot protein powder;
(5) maggot polypeptide powder and maggot protein powder are mixed, namely get the maggot compound protein powder.
The above only is embodiments of the invention; be not so limit claim of the present invention; every equivalent structure or equivalent flow process conversion that utilizes description of the present invention to do; or directly or indirectly be used in other relevant technical fields, all in like manner be included in the scope of patent protection of the present invention.
Claims (1)
1. maggot compound protein powder is prepared as follows:
(1) fresh and alive maggot is cleaned, drained, add 2.5 times of water, wear into the maggot slurry, 95 ℃ of sterilization 10min make the various enzyme deactivations in the maggot simultaneously;
(2) regulating maggot slurry pH4.0, is 1:350 adding pepsin by pepsin with maggot slurry mass ratio, carries out enzyme digestion reaction 2.5 hours at 40 ℃, and enzyme 8 minutes goes out under 95 ℃; Then, regulating maggot slurry pH8.0, is 1:350 adding trypsase by trypsase and maggot slurry mass ratio, carries out enzyme digestion reaction 1.5h in 55 ℃, and enzyme 8 minutes goes out under 95 ℃; Then at 4 ℃, refrigerated centrifuge 10min under the 4500g condition collects supernatant and sediment respectively;
(3) supernatant with step (2) gained concentrate, spray-drying, obtain the maggot polypeptide powder;
(4) sediment to step (2) gained clean, subzero 22 ℃ of freeze drying 20h, pulverization process, cross 80 mesh sieves, obtain the maggot protein powder;
(5) maggot polypeptide powder and maggot protein powder are mixed, namely get the maggot compound protein powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210084977 CN102578361B (en) | 2012-03-28 | 2012-03-28 | Maggot compound protein powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210084977 CN102578361B (en) | 2012-03-28 | 2012-03-28 | Maggot compound protein powder |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102578361A CN102578361A (en) | 2012-07-18 |
| CN102578361B true CN102578361B (en) | 2013-09-11 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210084977 Active CN102578361B (en) | 2012-03-28 | 2012-03-28 | Maggot compound protein powder |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102578361B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021125956A1 (en) * | 2019-12-17 | 2021-06-24 | Protix B.V. | Method for the conversion of insects into insect pulp and nutrient streams, insect pulp and nutrient streams obtainable by this method |
| US12225912B2 (en) | 2013-02-07 | 2025-02-18 | Bühler AG | Method to convert insects or worms into nutrient streams and compositions obtained thereby |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104206640A (en) * | 2014-09-11 | 2014-12-17 | 桂林市和胤祥新型材料有限公司 | Process for preparing fly larva albumen powder by enzymatic hydrolysis of fly larvae |
| FR3031115B1 (en) | 2014-12-31 | 2018-03-02 | Ynsect | PRODUCTION OF CHITINES FROM INSECTS BY ENZYMATIC HYDROLYSIS COMPRISING A COMBINATION OF PRELIMINARY STEPS |
| FR3031113B1 (en) | 2014-12-31 | 2018-03-16 | Ynsect | PROCESS FOR PRODUCTION OF PRODUCT (S) OF INTEREST FROM INSECT BY ENZYMATIC HYDROLYSIS |
| DK3240905T3 (en) | 2014-12-31 | 2019-07-08 | Ynsect | Bill Pulver |
| NL2027744B1 (en) * | 2019-09-17 | 2022-09-16 | Protix Bv | Hydrolysate of water soluble insect proteins |
| CN116508884A (en) * | 2023-05-10 | 2023-08-01 | 广东日和堂医药科技有限公司 | Five-cereal worm protein powder preparation system |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1141130A (en) * | 1995-12-18 | 1997-01-29 | 武汉神虫珍奇养生制品有限公司 | Nutrient active matter dried powder of edible engineering maggot, method for producing same |
| CN1297691A (en) * | 1999-12-01 | 2001-06-06 | 中国科学院动物研究所 | Separation and extraction process of effective component in resource insect |
| CN1415757A (en) * | 2002-11-20 | 2003-05-07 | 江南大学 | Method for extracting protein and chitin from fly maggot by using enzyme hydrolysis as well as preparing chitosan from chitin |
| CN1699405A (en) * | 2005-06-02 | 2005-11-23 | 上海交通大学 | Method for preparing low molecular weight insect protein by hydrolyzing fly maggot protein under pressure |
-
2012
- 2012-03-28 CN CN 201210084977 patent/CN102578361B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1141130A (en) * | 1995-12-18 | 1997-01-29 | 武汉神虫珍奇养生制品有限公司 | Nutrient active matter dried powder of edible engineering maggot, method for producing same |
| CN1297691A (en) * | 1999-12-01 | 2001-06-06 | 中国科学院动物研究所 | Separation and extraction process of effective component in resource insect |
| CN1415757A (en) * | 2002-11-20 | 2003-05-07 | 江南大学 | Method for extracting protein and chitin from fly maggot by using enzyme hydrolysis as well as preparing chitosan from chitin |
| CN1699405A (en) * | 2005-06-02 | 2005-11-23 | 上海交通大学 | Method for preparing low molecular weight insect protein by hydrolyzing fly maggot protein under pressure |
Non-Patent Citations (4)
| Title |
|---|
| 吴杰等.蝇蛆组织匀浆液及其酶解物中抑菌组分的研究.《江苏农业科学》.2007,(第4期),153-155. |
| 李玲玲等.蝇蛆生物活性肽的生产工艺研究.《家畜生态学报》.2008,第29卷(第3期),53-55,89. |
| 蝇蛆生物活性肽的生产工艺研究;李玲玲等;《家畜生态学报》;20080531;第29卷(第3期);53-55,89 * |
| 蝇蛆组织匀浆液及其酶解物中抑菌组分的研究;吴杰等;《江苏农业科学》;20071231(第4期);153-155 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US12225912B2 (en) | 2013-02-07 | 2025-02-18 | Bühler AG | Method to convert insects or worms into nutrient streams and compositions obtained thereby |
| WO2021125956A1 (en) * | 2019-12-17 | 2021-06-24 | Protix B.V. | Method for the conversion of insects into insect pulp and nutrient streams, insect pulp and nutrient streams obtainable by this method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102578361A (en) | 2012-07-18 |
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Effective date of registration: 20200602 Address after: Guli town Shuanggang village, Changshu City, Suzhou City, 215500 Jiangsu Province Patentee after: Changshu Guli Technology Venture Service Co., Ltd Address before: Changshou City miaoquan street Suzhou city Jiangsu province 215500 Patentee before: CHANGSHU HUIKANG FOOD FACTORY |