CN102526781A - Compound biological enzyme deodorant and preparation method thereof - Google Patents
Compound biological enzyme deodorant and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a compound biological enzyme deodorant and a preparation method thereof. The compound biological enzyme deodorant contains the ingredients consisting of one of laccase, hemicellulase and oxidoreductase and the mixture of proteinase, lipase, cellulose, pectinase and a filler and is prepared by carrying out the following working procedures of: enzyme selecting and compounding, standing and nitrification, low-temperature concentrating, molecular sieving, hierarchal filtering, fluidized bed low-temperature drying, molecular modification and immobilization, product forming, inspecting and packaging. The product prepared by the preparation method has the advantages of stable quality, remarkable deodorizing effect, longer keeping time, environment-friendliness, convenience in use and suitability for the air purification and deodorization of fields, such as the municipal facilities including public toilets, garbage dump stations, garbage transfer stations and the like, the public places including stations, guest houses, hotels and the like, the household facilities including kitchens, wash basins, shower compartments, siphon tubes or any drainage pipeline and the like, and the factory workshops and the like.
Description
Technical field
The invention belongs to the Environmental Biotechnology field, be specifically related to a kind of compound biological enzyme deodorizer and preparation method thereof.
Background technology
At present, deodorizer commonly used mainly contains following several types: physical deodorization agent, chemical deodorizing agent, microbiocidal deodorizer, plant type deodorizer and compound deodorizer etc.
The physical deodorization agent is to carry out deodorization through physical method, utilizes the physical property of deodorizer or foul smell, does not change the result of odor components, only changes the local concentration of foul smell, or perhaps relative concentration.Common the absorbing and deodorizing agent arranged, cover deodorizer etc.Shortcoming is to be difficult to change the foul smell gas componant fully, and people and animals, equipment and environment etc. are still had infringement to a certain degree.
Thereby the chemical deodorizing agent is to utilize oxidation, reduction decomposition, neutralization reaction, additive reaction, condensation reaction, ion-exchange reactions etc. that the odorant that produces is become the odorless material to destroy foul odors.Shortcoming is that investment is big, and operation cost is higher relatively, and particularly the product behind the chemical reaction has the probability that causes new uncontrollable environmental pollution.The case that the pollution that causes owing to chemosynthesis deodorizer in recent years causes happens occasionally; The somebody treats the preceding paragraph time spraying perfume or contained in the environment of deodorizer of fragrance; Symptoms such as skin pruritus, dizziness and cough can appear, the serious even asthma that can happen suddenly.The somebody pruritus dermatitis unbearably can occur once some aroma type deodorizer of contact, even feels sick, vomits.These are all relevant with the pollution of chemical deodorizing agent.
Natural plant deodorant is adopt to extract that the natural disinfection deodorization factor is refining in the plant forms.Have antibacterial, antibacterial and deodorization effect, stenches such as ammonia, hydrogen sulfide are had good decomposition removal effect, can be used for the air cleaning and the abnormal flavour processing of industries such as food medicine, solid useless processings, sewage disposal.Its effect changes the spatial configuration of abnormal flavour molecule, weakens the chemical combination key in the abnormal flavour molecule, easily and the acidic buffer generation chemical reaction in other molecules and the vegetation water, generates tasteless, nontoxic material at last.Its deficiency is, the plant deodorant extraction process is complicated, and production cost is higher, and the deodorization object is single, also possibly produce the chemical reaction that some are difficult to expect.
Biological deodorant is the metabolite that makes full use of beneficial microbe colony, adopts microbial fermentation, abstraction technique production, and it has the stench of imitating smelly sources such as obstruct feces and distributes.Its ultimate principle is to utilize microorganism to be absorbed in microorganism to the odorant in the dissolving water in health, makes a kind of process of its degraded through the metabolic activity of microorganism.Microbial deodorization can be divided into three phases: the 1. course of dissolution of foul gas, promptly transfer to liquid phase by gas phase; 2. odor pollutant is adsorbed, absorbs by microorganism in the aqueous solution; 3. the odor pollutant that gets into microbial cell is that Institute of Micro-biology decomposes utilization as nutrient substance, makes pollutant be able to remove.Yet; The pollutant that contain in the stink are very complicated mixed systems; Single culture can't reach satisfied effect, is difficult to establish design standard, and the pollution factor that has by-product after microbial metabolism is indeterminate; There is the Environmental security problem, and is not suitable for shortcomings such as extra heavy concentration foul smell.
Therefore, have the exploitation and the use of the deodorizer of efficient deodorizing effect and biological safety, become the domestic and international research focus, and have great market and ecological significance.Exploitation compound enzyme biological deodorant; Research and development through the biological fixnig system; Screening, make up efficient, cheapness, strong stress resistance, high-performance biological preparation safe in utilization; Make various enzyme performance synergism, widen the kind that can handle pollutant, improving treatment effeciency is the developing direction that the environment friendly biological formulation development is produced.
It is the broad-spectrum biological enzyme preparation that compound biological enzyme deodorizer manufacturing approach of the present invention obtains this product attribute, has special advantages.Through containing multiple meticulously preferred potentiation enzyme, multiple foul smell composition but synergism is degraded; Accessible stench inorganic compound mainly comprises sulfur dioxide, hydrogen sulfide etc.; Accessible volatility stench Organic substance mainly comprises sulfurous organic compound (mercaptan, thioether), phosphorus-containing matter, chlorinated organics etc.This product can be used for sewage, rubbish, efficient deodorizing product in the Treatment of Sludge; But the common stink factor in also degrade in the effective environment volatile fatty acid and the pollutant; Particularly biological oxidation enzyme and catabolic enzyme strengthen its deodorizing capability; Safe water based formulas and solid-state prescription, nontoxic to the person poultry safety, nonirritant; Not flammable, biodegradable, non-secondary pollution; Be applicable to room temperature, preference temperature is 10-50 ℃; Usually with water dilution 5-10 doubly, easy to use.
Summary of the invention
The object of the present invention is to provide a kind of compound biological enzyme deodorizer and preparation method thereof, the product processes of this method preparation is advanced, and material is easy to get, and cost is low, and enzyme is lived stable.
For realizing above-mentioned purpose, the present invention adopts following technical scheme:
A kind of component of compound biological enzyme deodorizer is a kind of and protease, lipase, cellulase, the pectase in laccase, hemicellulase, the oxidoreductase, the mixture of filler;
Described filler is cyclodextrin, Polyethylene Glycol, dextran, heparin or sucrose polymer;
The mass percent of each component is: proteinase 8-13%, lipase 9-15%, cellulase 10-15%, pectase 8-13%, laccase 12-15%, hemicellulase 9-12%, oxido-reductase 12-15%, filler 30-54%, each constituent mass percent sum is 100%.
Described compound biological enzyme deodorizer is a kind of in water preparation, aqueous emulsion, microemulsion, Powdered, graininess, the bulk.
A kind of method for preparing of compound biological enzyme deodorizer comprises following operation: select enzyme composite, leave standstill nitrated, cryoconcentration, molecule screening, level is filtered, ebullated bed cold drying, molecular modification and immobilization, molding, check, packing.
The concrete steps of its preparation method are following:
(1) different according to removal foul smell composition, selecting enzyme activity for use is enzyme at the enzyme of 500-1000U/mg, and each enzyme is pressed proportioning raw materials Weighing and material sorting;
(2) under 10-30 ℃; The pure water that the enzyme of selecting for use is added its 10 times of quality respectively dissolves, precipitates; Separated liquid supernatant; Under the 0.1-0.5MPa condition, be the ultrafilter membrane of 0.005-0.015 μ m through membrane aperture respectively then, make the enzyme liquid of unit of activity 2000-2500U/mL, pH5-6; Enzyme during tailings is slagged tap with the further dissolved of organic solvent is mixed in the enzyme liquid;
Described organic solvent is a kind of in ethanol, methanol, ethyl acetate, the hexone;
(3) under 40-50 ℃, the enzyme liquid of step (2) is fully stirred 30-40min in container, make it mix homogeneously; Leave standstill 2-6h under 20-35 ℃; Under the 0.1-0.5MPa condition; Through membrane aperture is the ultrafilter membrane of 0.005-0.015 μ m, and 25-35 ℃ of following concentrated thickening reaches the enzyme liquid of unit of activity 2500-3000U/mL, this enzyme liquid is passed through once more the ultrafilter membrane of 0.005-0.015 μ m; Making with extra care out unit of activity is enzyme mixed liquor preservation under-5 ℃ of 20000-5000000U/mL, is liquid compound biological enzyme deodorizer;
(4) with the enzyme mixed liquor of step (3) through dried, make solid, shaped compound biological enzyme deodorizer;
Described dried is vacuum drying, infrared drying, airpillow-dry, lyophilization or adds the inserts natural air drying;
The technological parameter of described airpillow-dry is that solid concentration is 3-5%, and the sample introduction flow is 360mL/h, and EAT is 40 ℃-50 ℃, and outlet temperature is 30 ℃～35 ℃.
Biological enzyme technology deodorization mechanism: the stink major ingredients is hydrogen sulfide, methanthiol, methyl sulfide, dimethyl disulfide etc., is referred to as total reduced sulfur, wherein also contains polluters such as indoles organic compound and Hydrocarbon.Sulfide composition is complicated, comprises inorganic sulfur and organic sulfur etc.Enzyme reacts through participating in carrying out albuminolysis reaction, amylolysis reaction and oxidized fibre, and polysaccharide and the intermolecular combination reaction of foul smell, and the enzyme decomposition produces the strand of malodorous compounds etc., thereby reaches the purpose of removing stink.Nitrogenous organic substance such as amine, indoles are emitted NH through elite biological enzyme Ammonification
3, NH
3Can be oxidized to NO by biological enzyme
2 -, further be oxidized to NO again by biological enzyme
3 -The odorant of sulfur-bearing is oxidized into sulphuric acid by biological enzyme after the biological enzyme decomposition discharges H2S, organic substance etc. are decomposed into CO by elite enzyme
2, H
2O.Efficient compound bio enzyme preparation catalyzing deodorizing technology has unique treatment effect to foul smell, especially poisonous and harmful, the macromolecular compound that is difficult to degrade is had good degradation effect.This method is the application in the environmental pollution improvement field of enzyme and zymotechnic; Can carry out catalytic oxidation to the Organic substance that forms stink rapidly at short notice; Macromolecule organic open loop chain rupture behind catalytic oxidation forms small organic molecule, removes the polluter of the stink that produces etc.
Contain multiple enzyme in the enzyme deodorizer of the present invention; Be mainly a kind of in oxidoreductase, transferring enzyme, hydrolytic enzyme, lyase, isomerase, the ligase or some kinds; Odorant in the environment is broadly divided into three kinds: the one, and nitrogen-containing compound is like ammonia, trimethylamine, pyridine etc.; The 2nd, sulfur-containing compound is like hydrogen sulfide, methanthiol, thioether etc.; The 3rd, organic acid and aldehyde compound are like formaldehyde, isovaleric acid, propyl group .beta.-methylacrylic acid etc.Enzyme in the enzyme deodorizer of the present invention can neutralize with active group in the odorant such as sulfydryl, amido, ammonia etc., condensation reaction, suppresses sulfate simultaneously and transforms and generate hydrogen sulfide, eliminates being free in the stink substance in the environment.
To municipal administrations such as public lavatory, rubbish station, garbage transfer stations; Public place such as station, hotel, families such as kitchen, hand washing sink, shower cabinet, siphon or any drainage pipeline, and the optimum enzyme of the full spectrum of varying environment such as factory floor air cleaning deodorization combination; Can, multiple polluter play a role when existing; Biological activity is high, and it is through biological enzyme degradation function degraded osmophore and suppress the foul smell conversion, realizes removing the purpose of stink.
(1) the contained elite enzyme of product of the present invention can neutralize with active group in the odorant such as sulfydryl, amido, ammonia etc., condensation reaction; Suppress sulfate simultaneously and transform generation hydrogen sulfide; Polluter in the foul smell is converted into harmless carbohydrate; Elimination is free in the stink substance in the environment, can not cause any secondary pollution.
(2) realized the optimum organization of enzyme, its effect is that other deodorizer are inaccessiable.Select suitable prescription, its deodorization is assessed, the various odor pollutants that comprise ammonia, hydrogen sulfide, methanthiol, thioether etc. are shown good removal effect.
(3) treatment effeciency is high.Product of the present invention is compared with general chemical method and biological method, is 100 times of traditional method to the degradation effect of odorant, can remove stink rapidly, purifies air.
(4) adaptability is wider.Product of the present invention can adapt to various temperature and pH scope, in low-oxygen environment, also can effectively play a role.
(5) more targeted.Product of the present invention can be adjusted multiple formulations under the main formula prerequisite, can be adaptable across different field, heterogeneity foul smell.
(6) treatment cost is low.Product of the present invention has the characteristics of treating both the principal and secondary aspects of a disease, and comprehensive control cost and dynamic investment cost are minimum, and regulation effect is remarkable.
(7) pure Green Product.Product of the present invention all adopts the pure-natural biological enzyme to be composited, and does not contain any chemical drugs, does not also contain the transgenic product composition, can not cause secondary pollution, and is harmless to human non-toxic, representing the following direction of biological environmental production industry development.
Therefore, not only deodorizing effect is good for enzyme deodorizer of the present invention, advanced technology, and suitable industrial-scale production and application market popularization.
Each processing step and advantage are in the manufacturing approach of the present invention:
1) selects the enzyme complex process
The optimum enzyme of the full spectrum of varying environment combination can play a role when multiple polluter exists, and biological activity is high, and it passes through biological enzyme degradation function degraded osmophore and suppress foul smell to transform, and realizes the purpose of removal stink.A kind of pollutant can dispose corresponding enzyme and effectively handle.Be mainly a kind of in oxidoreductase, transferring enzyme, hydrolytic enzyme, lyase, isomerase, the ligase or some kinds.
Soak: 10～30 ℃ of operating ambient temperatures; The storage tank jar; Stir: treatment temperature: 40～50 ℃; Processing time: 30～40min; Extracting: fill is stored: 25kg, 120kg bucket packing, be stored in 0 ℃-20 ℃ the warehouse, and stable phase stores 1 year.The enzyme compound formulation can be preserved under 37 ℃ 90 days, and this enzyme and compound formulation thereof do not contain the chemical disinfection composition, can not cause secondary pollution to environment.Said preparation is nontoxic, non-stimulated.
In compound process; The monomer type and the synthesis technique that use when selecting different living enzyme polymerization; Make final synthetic enzyme agent in molecular weight, molecular weight distribution and chain structure in aqueous solution, through the synthetic macromolecule compound enzyme agent of free-radical polymerized principle with comb-type structure.2) leave standstill digestion process
This technology is under aerobic or anaerobic condition, material to be carried out in the standing and reacting tank body, and the time is 2-6 hour, and temperature is controlled at 20-35 ℃, reaches and stablizes the effect of complex enzyme power, and each is component balanced to make the product intermediate.And the quality that reduces the residual slag charge of preparation process again and have nothing to do with reactant.The oneself of biotic environment system keeps balance.Can make the product stabilisation, make it be difficult for to change, safe and harmlessization, and improved the effect of product.
3) low-temperature concentration process
Adopt membrance separation to concentrate and combine with the production environment air-conditioning, form a kind of energy-saving low-temperature concentration technology, material keeps producing the clean environment of knot, per hour composite extracting solution is concentrated to volume 10 multiple proportions example from initial mass concentration.Concentrate operating ambient temperature 25-35 ℃, concentrated temperature of charge is controlled at below 50 ℃, and the energy consumption of this low-temperature concentration process is energy-conservation 30%, and service life of equipment prolongs; Tail washings is back in the extraction pot under the atmospheric pressure state automatically times without number, concentrates the difficult problem of temperature drift when solution enzyme liquid concentrates while extracting.4) molecular sieve division technique
Utilize molecular sieve can enzyme liquid be separated with non-utilization organic matter.The enzyme molecule itself has the protein tridimensional network, and big molecule resistance through the hole on this RF time is bigger, and resistance was less when micromolecule passed through.When selecting the sifting bed that molecular weight 20,000-5,000,000 U varies in size for use, according to molecular weight size ordered distribution.Molecular sieve can improve the activity and the uncommon selectivity of enzyme, effectively guarantees the enzyme quality.
5) level filtering technique
A centrifugalize: remove the large granular impurity and the mineral-type solids precipitation thing that in composite enzyme liquid, contain.B vibrates sub-sieve: the cotton-shaped impurity of colloid of further removing float and course of reaction generation through the material of centrifugalize.C adsorption precipitation: make the enzyme molecule fully obtain activating release and being retained in the aqueous based carrier through adding activator (activated calcium etc.) material after above-mentioned supplied is accomplished.D fine straining ultrafiltration: adopt the fine straining hyperfiltration technique further to remove colloidal substance and denatured protein in the material, make enzyme liquid obtain clarification, keep molecular weight 20,000 U-5,000,000 U.Can adopt the ultra rate unit of series connection or parallel connection, in airtight environment, carry out ultrafiltration and can significantly reduce the influence of oxygen, the enzyme of product intermediate lived be able to more completely keep with effect to enzyme liquid quality.This cell process is not pursued single enzyme preparation purity, but (concentration) of raising compound enzyme compares vigor.
This technology makes enzyme preparation material enzyme quality in the course of processing improve greatly, effectively removes the many unfavorable factors that influence quality, and the product vigor that obtains is higher; Room temperature state enzyme is lived in losing and is lacked; And can guarantee the production high efficiency, smooth operation is beneficial to the prolongation in plant maintenance and service life.
6) ebullated bed low temperature drying technology
Material after above-mentioned PROCESS FOR TREATMENT adopts boiling drier, claims fluid bed again, generally is made up of heater, ebullated bed main frame, cyclone separator, sack cleaner, air-introduced machine, operating board.According to the different in kind of material, can match cyclone separator or sack cleaner on demand.
Boiling drier is to utilize air after heat exchanger heats, forms hot blast and distributes the entering main frame through valve plate, and wet stock gets into drying machine from feeder; Because the effect of blast, material forms fluidized state in drying machine, and extensively contacts with hot-air; Confirm that the optimal performance technological parameter is that solid concentration is 3-5%; The sample introduction flow is 360mLh, and EAT is 40 ℃-50 ℃, and outlet temperature is 30 ℃～35 ℃; Enzyme loss rate about 2% alive is accomplished drying materials at the short period cryogenic conditions.This technology guarantees effectively that enzyme is lived and does not suffer a loss.
7) molecular modification and immobilization technology
Increase compound enzyme biological preparation stay in grade.Introducing through chemical group or remove changes the protein covalent structure.Under relatively mild condition, make a kind of protein play specific reaction with the mode that can control, thereby cause the chemical modification of single amino acids residue or its functional group generation covalency with some chemical reagent.There are a plurality of reactive groups in the dressing agent molecule, can to form multiple spot crosslinked with enzyme.Make the native conformation of enzyme produce " rigidity " structure.Make enzyme molecular structure be in a kind of poised state at last, closely orderly conformation is maintained.Enhanced stability, the inactivation of minimizing enzyme.It is efficiently quick through this technology the directed target function of compound enzyme to be improved the soiled decomposition of dirt greatly.Removing influences enzyme quality unfavorable factor, the enzyme deodorising product that obtains efficiently expressing.
Steady-state conditions and optimum reaction condition optimum pH are 4.5, temperature is 45 ℃.Dressing agent is: a kind of in cyclodextrin, Polyethylene Glycol (PEG), dextran (dextran), heparin (heparin), the sucrose polymer (Ficoll).The activator that adopts: a kind of in CNBr method, glutaraldehyde method, the azide method.
Graininess compound enzymic preparation immobilization technology: adopt the covalent bond method to connect with forming chemical covalent bond on the pheron molecule between the lip-deep reactive group of functional group and solid support; Be connected firmly between enzyme and carrier; Be difficult for taking place enzyme and come off, good stable property and reusability are arranged.It adopts carrier to comprise natural polymer (cellulose, agarose, starch, glucose gel, collagen and derivant etc.) and inorganic support (cellular glass, metallic oxide etc.).Compound enzyme material through this workshop section PROCESS FOR TREATMENT well keeps perhaps having prolonged the enzyme activity unit time.
8) preparations shaping technology
This compound enzyme biological deodorant, above-mentioned technical process are the basic features of present technique, but can be according to different needs, and all or part of method in the reservation technology is to adapt to dosage form.Can be prepared into water preparation, solid formulation (comprising block tablet), agent gas.
Multi-pharmaceutics can be suitable for the problem that exists in the process of consumption, through forming technique enzymatic activity is further protected, and prolongs the storage life of preparation within the specific limits.The research of storage-stable shows, in the substrate, has reduced the sensitivity to the preservation temperature, has improved oxidation resistance, and the enzyme activity half-life is able to prolong.
In sum, this invention is compared with present prior art, and production technology design of the present invention is tight; With high content of technology; The excellent product quality of producing is a kind of novel modern enzyme and zymotechnic new application in the product of environmental protection field, has integrate innovations characteristics.To municipal administrations such as public lavatory, rubbish station, garbage transfer stations; Public place such as station, hotel, families such as kitchen, hand washing sink, shower cabinet, siphon or any drainage pipeline, and the optimum enzyme of the full spectrum of varying environment such as factory floor air cleaning deodorization combination; Can, multiple polluter play a role when existing; Biological activity is high, and it is through biological enzyme degradation function degraded osmophore and suppress the foul smell conversion, realizes removing the purpose of stink; And environmentally friendly, do not produce secondary pollution.
The specific embodiment
Describe in conjunction with the embodiment specific embodiment:
Below in conjunction with embodiment further explain advantage of the present invention and characteristics.Following examples only are exemplary, and those skilled in the art can make conspicuous modification on the basis, and these modifications also are included in the scope of the present invention.
Different according to the foul smell composition; Selecting enzyme activity a kind of or some kinds of enzymes more than 500U/mg for use is enzyme, with each enzyme component by weight ratio Weighing, material sorting, dissolution precipitation, leave standstill that digestion, separation and Extraction, mixing are stirred, refining purification, level filter, concentrate thickening, screening, drying, molecular modification and immobilization, get product.Wherein, the kind of enzyme preparation: oxidoreductase, transferring enzyme, hydrolytic enzyme, lyase, isomerase, ligase.Selecting for use of enzyme preparation: according to the characteristic of handling foul smell, selected enzyme preparation is purified with composite, and adsorbent is active carbon, silica gel, kieselguhr, aluminium oxide and various ion exchange resin etc.; Extractant is alcohols, acetate esters and first isobutyl ketone etc.Biological enzyme formulation is aqueous, water preparation, aqueous emulsion, microemulsion or Powdered, graininess or bulk.
Embodiment 1
According to the disclosed concrete grammar of claim, prepare a kind of enzyme deodorizer, this kind deodorizer belongs to rubbish station series of biologic enzyme deodorizer.Undertaken by the above-mentioned specific embodiment, it comprises each component of following weight ratio:
| Composition | Protease | Lipase | Cellulase | Pectase | Laccase | Filler |
| Content | 10-13% | 12-15% | 12-15% | 10-12% | 12-15% | 30-44% |
The vigor of various enzymes is all more than 500U/mg in the above component.
When above-mentioned enzyme deodorizer was used for the foul smell processing of rubbish station, the enzyme that contains in the enzyme deodorizer is catalytic degradation osmophore and the conversion of inhibition foul smell effectively, thereby removes stink.
Refuse landfill rubbish can produce a large amount of foul smell in the landfill process, influence surrounding enviroment.It is that domestic waste is concentrated the place of filling out the reason processing that rubbish is filled out Li Chang; Owing to here topple over the process of processing from the rubbish that each rubbish point ships; Especially in summer, autumn; Organic substance in the house refuse decomposes harmful odorous gas such as producing a large amount of ammonias, hydrogen sulfide, amine, aromatic hydrocarbons, alcohols, organic acid, indole, has been full of rubbish and has filled out the whole space of managing the field, and foul smell causes ambient air contamination.About 100 0m2 of landfill district job area.Adopt this compound biological enzyme cleanser deodorize liquid, through the rubbish surface of atomizing spray to landfill, and in the rubbish toppling process, spray fast, thereby reach the deodorant effect.A eliminates the stink that Organic substance decomposes and animal wastes produce in the house refuse; B disappears and murders worm: mosquito, fly blow and larva are had certain killing action, and minimizing mosquito, fly gather; C can increase compactness, thereby increases the reason ability etc. of filling out that rubbish is filled out the reason field.D, biological enzyme formulation are pure biological product, can 100% biodegradation, and safe in utilization, can not cause secondary pollution to environment; E because of biological enzyme formulation is the compound enzyme that contains multiple difference in functionality enzyme, when being used for the house refuse of complicated component, more showing it and acts on many target spots deodorant than powerful.Under conditions such as suitable temperature, humidity, in rubbish or percolate, can keep the efficient catalytic effect; F has certain accumulative effect spraying continuously when using, thereby can reduce consumption but do not reduce result of use; G, biological enzyme formulation with water be solvent can be according to actual needs by the arbitrary proportion dilution, as long as be equipped with suitable spray appliance, can use very easily in various different places such as the pour(ing) point in soot, diafiltration liquid pool, roads.
Embodiment 2
According to the disclosed concrete grammar of claim, prepare a kind of enzyme deodorizer, this kind deodorizer belongs to public place series of biologic enzyme deodorizer such as station, hotel.Undertaken by the above-mentioned specific embodiment, it comprises each component of following weight ratio:
| Composition | Protease | Lipase | Cellulase | Pectase | Hemicellulase | Filler |
| Content | 9-12% | 10-13% | 10-13% | 8-10% | 9-12% | 40-54% |
The vigor of various enzymes is all more than 500U/mg in the above component.
When above-mentioned enzyme deodorizer was used for family and the processing of public place foul smell, the enzyme that contains in the enzyme deodorizer is catalytic degradation osmophore and the conversion of inhibition foul smell effectively, thereby removes stink.
Embodiment 3
According to the disclosed concrete grammar of claim, prepare a kind of enzyme deodorizer, this kind deodorizer belongs to family's series of biologic enzyme deodorizer such as kitchen, hand washing sink, shower cabinet, siphon or any drainage pipeline.Undertaken by the above-mentioned specific embodiment, it comprises each component of following weight ratio:
| Composition | Protease | Lipase | Cellulase | Pectase | Oxidoreductase | Filler |
| Content | 8-10% | 9-12% | 10-14% | 10-13% | 12-15% | 36-51% |
The vigor of various enzymes is all more than 500U/mg in the above component.
When above-mentioned enzyme deodorizer being used for foul smell such as kitchen, hand washing sink, shower cabinet, siphon or any drainage pipeline and handling, the enzyme that contains in the enzyme deodorizer is catalytic degradation osmophore and suppress foul smell and transform effectively, thus the removal stink.
Embodiment 4
According to the disclosed concrete grammar of claim, prepare a kind of enzyme deodorizer, this kind deodorizer belongs to factory floor series of biologic enzyme deodorizer.Undertaken by the above-mentioned specific embodiment, it comprises each component of following weight ratio:
| Composition | Protease | Lipase | Cellulase | Pectase | Oxidoreductase | Filler |
| Content | 9-11% | 12-15% | 11-14% | 9-12% | 12-15% | 33-47% |
The vigor of various enzymes is all more than 500U/mg in the above component.
When above-mentioned enzyme deodorizer was used for the processing of factory floor foul smell, the enzyme that contains in the enzyme deodorizer is catalytic degradation osmophore and the conversion of inhibition foul smell effectively, thereby removes stink.
The foregoing description can be found out; Enzyme deodorizer of the present invention shows good removal effect for the various odor pollutants that comprise ammonia, hydrogen sulfide, methanthiol, thioether etc.; Simultaneously for comprise in refuse receptacle, lavatory, the automobile, the common common abnormal flavour environment of smoke, also show tangible deodorizing effect.Through the synergism and the optimum organization of enzyme, all show excellent deodorization effect for common foul gas and abnormal flavour environment.This product is the compound bio enzyme preparation, when being used for the workshop organic exhaust gas of complicated component, more showing it and acts on many target spots deodorant than powerful.In the wide temperature of ambient temperature 10-50, possess under the damp condition, can keep catalytic action efficiently, quicken organic decomposition; Can 100% biodegradation.Environmentally safe, efficient, nontoxic; Even get in the eye or injured skin, get final product with flushing with clean water, operator safety is harmless.
Embodiment 5
Compound biological enzyme is directly added by a certain percentage in smelly water body, and compound biological enzyme can be present in water body and the mud through from immobilization and compound immobilization role for a long time, is difficult for running off.Improvement phase dosage (3 days): strengthen to add according to the water yield and with reference to the ratio of sludge quantity; Maintenance phase dosage (1 week): proportionally add.Can no longer add after up to standard.The follow-up maintenance phase (180-360 days): ratio adds as required, and purpose is to activate the metabolic process of the efficient probiotics of microorganisms in water, thereby keeps the long-term clean-up effect of water body.Rapidly and efficiently catalytic degradation produces the pollutant of stink, removes stink; Shorten the processing cycle, thereby reduce operating cost; Improve the surrounding enviroment quality, eliminate abnormal flavour, prevent the breeding of flies and mosquitoes; Simultaneously can efficiently remove the pollutant in the mud, reduce sludge quantity, reduce Facilities Construction, and can make processing system under wide temperature, bring into play the effect of biodegradation pollutant; A kind of reinforcement to nature recovery capability and self-purification capacity.Compare with other treatment technologies, really realize unpowered or micro power consumption, meet the developing direction of energy-saving and emission-reduction and low-carbon economy.
Embodiment 6
(1) soaks haydite after 1-1.5 hours with 8% hydrochloric acid solution, put dried solution and add clear water (preferably pure water) washing to not residual.(2) recording the PH that haydite is immersed in the water is below the neutrality.Best between 5-6.(3) haydite after will washing is dry (is preferably in 100-140OC).Make the central no hydrone of hole and other non-reacted residue of haydite.(4) dried haydite was soaked in the enzyme liquid 6-7 hours at least, Best Times 12 hours wherein, slowly stirs 0.5-1 hour (12-24 rev/mins of mixing speeds).(5) will pass through the pottery material of enzyme liquid in soaking, handle 0.5 hour through the way transformation that vacuum is taken out.(6) haydite is taken out from enzyme liquid or enzyme liquid is entered other storage tank after, carry out the haydite drying, temperature must not surpass 45oC.(7) enzyme liquid carries out secondary and applies mechanically the immersion haydite.Enzyme activity is detected with the sampling of dried finished products enzyme filler in (operation is pressed said process) (8).(9) with dried enzyme filler dress post (or pack), subsequent use.
The present invention is novel, with the enzyme deodorizer that this invention makes, is applied to municipal administrations such as public lavatory, rubbish station, garbage transfer station; Public place such as station, hotel, families such as kitchen, hand washing sink, shower cabinet, siphon or any drainage pipeline, and in the field such as factory floor air cleaning deodorization; The deodorization good purification; Efficient height and cost are low, and stability is strong, and is easy and simple to handle.
Above-mentionedly be merely several specific embodiment of the present invention; Should be pointed out that design concept of the present invention is not limited thereto, for those skilled in the art; Under the prerequisite that does not break away from the principle of the invention; Can also make some improvement and retouching, allly utilize this design that the present invention is carried out the improvement and the retouching of unsubstantiality, all should belong to the behavior of invading protection domain of the present invention.
Claims (4)
1. compound biological enzyme deodorizer is characterized in that: the component of described compound biological enzyme deodorizer is a kind of and protease, lipase, cellulase, the pectase in laccase, hemicellulase, the oxidoreductase, the mixture of filler;
Described filler is cyclodextrin, Polyethylene Glycol, dextran, heparin or sucrose polymer;
The mass percent of each component is: proteinase 8-13%, lipase 9-15%, cellulase 10-15%, pectase 8-13%, laccase 12-15%, hemicellulase 9-12%, oxido-reductase 12-15%, filler 30-54%, each constituent mass percent sum is 100%.
2. compound biological enzyme deodorizer according to claim 1 is characterized in that: described compound biological enzyme deodorizer is a kind of in water preparation, aqueous emulsion, microemulsion, Powdered, graininess, the bulk.
3. the method for preparing of a compound biological enzyme deodorizer as claimed in claim 1, it is characterized in that: described method for preparing comprises following operation: select enzyme composite, leave standstill nitrated; Cryoconcentration, the molecule screening, level is filtered; The ebullated bed cold drying, molecular modification and immobilization, molding; Check, packing.
4. the method for preparing of compound biological enzyme deodorizer according to claim 3 is characterized in that: the concrete steps of described method for preparing are following:
(1) different according to removal foul smell composition, selecting enzyme activity for use is enzyme at the enzyme of 500-1000U/mg, and each enzyme is pressed proportioning raw materials Weighing and material sorting;
(2) under 10-30 ℃; The pure water that the enzyme of selecting for use is added its 10 times of quality respectively dissolves, precipitates; Separated liquid supernatant; Under the 0.1-0.5MPa condition, be the ultrafilter membrane of 0.005-0.015 μ m through membrane aperture respectively then, make the enzyme liquid of unit of activity 2000-2500U/mL, pH5-6; Enzyme during tailings is slagged tap with the further dissolved of organic solvent is mixed in the enzyme liquid;
Described organic solvent is a kind of in ethanol, methanol, ethyl acetate, the hexone;
(3) under 40-50 ℃, the enzyme liquid of step (2) is fully stirred 30-40min in container, make it mix homogeneously; Leave standstill 2-6h under 20-35 ℃; Under the 0.1-0.5MPa condition; Through membrane aperture is the ultrafilter membrane of 0.005-0.015 μ m, and 25-35 ℃ of following concentrated thickening reaches the enzyme liquid of unit of activity 2500-3000U/mL, this enzyme liquid is passed through once more the ultrafilter membrane of 0.005-0.015 μ m; Making with extra care out unit of activity is enzyme mixed liquor preservation under-5 ℃ of 20000-5000000U/mL, is liquid compound biological enzyme deodorizer;
(4) with the enzyme mixed liquor of step (3) through dried, make solid, shaped compound biological enzyme deodorizer;
Described dried is vacuum drying, infrared drying, airpillow-dry, lyophilization or adds the inserts natural air drying;
The technological parameter of described airpillow-dry is that solid concentration is 3-5%, and the sample introduction flow is 360mL/h, and EAT is 40 ℃-50 ℃, and outlet temperature is 30 ℃～35 ℃.
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| CN103421264A (en) * | 2012-05-15 | 2013-12-04 | 罗门哈斯公司 | Enzymatic conversion of volatile organic compounds |
| CN104857843A (en) * | 2015-04-28 | 2015-08-26 | 孙祥章 | Biological enzyme air purifying agent, preparation method and application thereof |
| CN105936570A (en) * | 2016-07-12 | 2016-09-14 | 福州晨翔环保工程有限公司 | Chemical synthetic pharmaceutical wastewater treatment device and method |
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