CN102524792B - Bacterial cellulose particle product containing astaxanthin - Google Patents
Bacterial cellulose particle product containing astaxanthin Download PDFInfo
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- CN102524792B CN102524792B CN201110350050.9A CN201110350050A CN102524792B CN 102524792 B CN102524792 B CN 102524792B CN 201110350050 A CN201110350050 A CN 201110350050A CN 102524792 B CN102524792 B CN 102524792B
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials
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- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to a bacterial cellulose particle product containing astaxanthin. The astaxanthin is uniformly dispersed in fine reticular structures of bacterial cellulose to obtain the unique bacterial cellulose particle product containing the astaxanthin, the bacterial cellulose particle product containing the astaxanthin can be used in health care products to be eaten by customers, and the effect of preventing the skin aging is obtained. The problem that the astaxanthin cannot be easily stored for a long time under the ordinary storage conditions is perfectly solved. Meanwhile, a very good health care product which has the anti-aging function and can provide the dietary fiber is provided for the customers. The property of the astaxanthin in the bacterial cellulose particle product is very stable, the structure cannot be damaged, the astaxanthin can be slowly released for a long time after the eating, good effects of aging resistance, oxidation resistance and winkle resistance can be realized, and the effects of weight reduction and skin whitening can also be realized after the long-time eating.
Description
Technical field
The present invention relates to a kind of healthcare product.
Background technology
Astaxanthin, is from river crayfish shell, a kind of Red carotenoids of finding in oyster and salmon, and chemical name is 3,3 '-dihydroxyl-4,4 '-diketo-β, β '-carotene, pigment Aj067-69, CAS No:472-61-7, molecular formula C40H52O4, molecular weight 596.86.Can be with protein bound in vivo blue or green, blue.There is anti-oxidant, anti-ageing, antitumor, prevention cardiovascular and cerebrovascular diseases effect.Be that the mankind find the antioxidant that nature is the strongest up to now, its anti-oxidant activity is considerably beyond existing antioxidant.
The source of astaxanthin is mainly divided into chemosynthesis and natural extract.Conventional is natural extract method, normally from processing of aquatic products refuse, cultivation algae and yeast, extracts astaxanthin, and output is all lower.Astaxanthin can make from carotene by chemical process.Natural astaxanthin is mainly from Haematocoocus Pluvialls.
Research shows, astaxanthin has special role to Transglutaminase EC2.3.2.13 (transglutaminase), can when skin is subject to illumination, consume putrescine, and oral astaxanthin is stronger than oral vitamin A to the restraining effect of putrescine accumulation.Therefore, the strong anti-oxidation of astaxanthin may make it become potential bright protective agent, effectively removes the free radical that causes skin aging, and Cell protection film and mitochondrial membrane are avoided oxidative damage, for stoping skin photoage.
The Health Products Enterprises in the whole world has also released that about astaxanthin of money more than 200 is soft, the protective foods of hard capsule, oral liquid.But the special molecular structure of astaxanthin has determined to be also easy to be subject to the effect of light, heat, oxide compound etc. and has destroyed.Character is unstable, easy loss of activity in cosmetic preparation.
Summary of the invention
For above-mentioned defect, the invention provides a kind of bacteria cellulose particle product that contains astaxanthin.
Bacteria cellulose is the synthetic cellulosic general designation of certain micro-organisms in acetic acid Pseudomonas (Acetobacter), Agrobacterium (Agrobacterium), rhizobium (Rhizobium) and Sarcina (Sarcina) etc., it can form unique hyperfine reticulated structure by the biosynthesizing effect of microorganism.Had a lot of distinguished characteristics, as the Young's modulus of bacteria cellulose is more than the several times to ten of vegetable fibre times, and tensile strength is high; Bacterial fibers have very strong water-holding power.The WRV value of the bacteria cellulose of undried is up to more than 1000%, and the water-holding power after lyophilize still surpasses 600%.The ability of swelling again and linters through 100 ℃ of dried bacteria celluloses in water are suitable; Bacterial fibers have higher biocompatibility, adaptability and good biodegradability, is also extraordinary food fibre source.The present invention utilizes the above-mentioned characteristic of bacteria cellulose, by astaxanthin being dispersed in uniformly in the meticulous reticulated structure of bacteria cellulose, obtain the bacteria cellulose particle product of unique astaxanthin-containing, can, for edible for human consumer in healthcare products, obtain the effect that prevents skin aging.Solve well astaxanthin and under general preservation condition, be difficult for the defect of prolonged preservation, offered again a kind of extraordinary healthcare product that there is antisenility function, food fibre can be provided of human consumer simultaneously.Astaxanthin character in described bacteria cellulose particle product is highly stable, structure can not destroyed, after edible, can slowly and muchly discharge astaxanthin, play good anti-ageing, anti-oxidant, crease-resistant effect, long-term taking can also play the effect of fat-reducing, whitening.
The astaxanthin using in the present invention can be natural astaxanthin or synthesizing astaxanthin.The astaxanthin that is preferably natural extract, effect is best.
In order to play better health-care effect, bacteria cellulose particle product of the present invention, can also contain other appropriate nutritive ingredients, these nutritive ingredients include but not limited to have L-carnitine and/or the oxysuccinic acid of antiobesity action, can also contain appropriate VITAMIN.
The preparation method of the bacteria cellulose particle product that contains astaxanthin of the present invention, is that the astaxanthin emulsion of mass concentration 1% is added in fermentation medium for bacterial cellulose, and making the content astaxanthin in substratum is 5-200ppm; Recycle this fermention medium static state or dynamic fermentation and produce bacteria cellulose, then the bacterial fibers film cutting that static fermentation is obtained or be milled to particulate state; Or dynamic fermentation directly obtains bacterial fibers crude granule, finally obtain the bacteria cellulose particle product of astaxanthin-containing.The bacteria cellulose particle product obtaining thus can be enclosed in astaxanthin in its meticulous reticulated structure, is able to the stable of maintain property; In use, can slowly and muchly discharge again.If need to add other nutritive substances, be added in fermention medium to carry out biosynthesizing, as being also added with L-carnitine and/or oxysuccinic acid in substratum, wherein the addition of L-carnitine is that to make the content in substratum be 5-100mg; The addition of oxysuccinic acid is that to make the content in substratum be 10-500mg.
L-carnitine wherein, another name L-BETAIN, DL-carnitine chloride, chemical name beta-hydroxy γ-TMA (TriMethylAmine) butyric acid, is a kind of white lens or white transparent fine powder.L-carnitine is the moisture absorption very easily, and stability can be placed more than 1 year preferably in the solution of pH 3-6, high temperature that 200 ℃ of abilities are above and have good solubization and water-absorbent.The main Physiological Function of L-carnitine is to promote that fat changes into energy, taking L-carnitine can be when reducing body fat, reducing body weight, do not reduce moisture and muscle, at the international fat health tissues of 2003 Nian Bei, regard as the safest fat-reducing nutrient complementary goods having no side effect.L-carnitine is added in fermention medium, in the biosynthetic process of bacteria cellulose, be wrapped in reticulated structure, in use, obtain slowly and muchly discharging together with astaxanthin, not only can effectively prevent the L-carnitine moisture absorption, can work in coordination with and play very effective anti-ageing, crease-resistant, antiobesity action with astaxanthin, bacteria cellulose again.
Wherein oxysuccinic acid, has another name called 2-hydroxy-butanedioic acid, naturally with three kinds of forms, exists, i.e. D-malic acid, L MALIC ACID and its mixture D L MALIC ACID.For white crystals body or crystalloid powder.The tart flavour that has special happiness.L MALIC ACID is the tricarboxylic intercycle body of organism, and mouthfeel approaches straight juice and has natural flavor, compares with citric acid, and the heat of generation is lower, and taste is better, is therefore widely used in the numerous food such as drinks, beverage, jam, chewing gum.In L MALIC ACID, also contain natural moisturizing composition, can dissolve at an easy rate " adhesive " that be bonded between dry lepidiod dead cell, thereby can remove skin surface wrinkle, make skin become tender white, bright and clean and flexible.Recently also there are some researches show, oxysuccinic acid and L-carnitine coupling can be played extraordinary antiobesity action.Coupling bacteria cellulose of the present invention, astaxanthin and oxysuccinic acid and/or L-carnitine, can prevent the oxysuccinic acid moisture absorption; If with L-carnitine coupling, long-term taking can play the health-care effect of significant fat-reducing, anti-oxidant, anti-ageing, whitening.
In above-mentioned preparation method, the fermented bacterium of bacteria cellulose is preferably acetobacter xylinum or gluconate pyracetobacillus.In fermention medium, contain the enough Carbon and nitrogen sources for strain growth, carbon source is preferably glucose, fructose, sucrose and/or Sucus Cocois.
Bacteria cellulose particle product of the present invention is preferred for preparing protective foods, as nourishing drink, jelly production, jam product, milk preparation, frozen food confection frozen goods membranogen in use, can slowly and muchly discharge again.If need to add other skin cares etc.Be suspended in product, can obtain there is desirable mouth feel, can complementary diets fiber, there is the novel healthy food of the function such as anti-oxidant.Can eat at any time.
Embodiment
Below in conjunction with embodiment, the specific embodiment of the present invention is described in further detail.Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.
Embodiment 1:
A product, it is that the bacteria cellulose uniform particles that contains astaxanthin is suspended in beverage.
The preparation method of the above-mentioned bacterial fibers crude granule that contains astaxanthin is as follows:
By the natural left-handed astaxanthin emulsion of mass concentration 1% (emulsification of Haematocoocus Pluvialls extract) ASTA-W1(operative norm Q/YSD03-2009) (purchased from Jingzhou City natural astaxanthin company limited) be added in fermentation medium for bacterial cellulose, and making the content astaxanthin in substratum is 200ppm; In fermention medium, also contain the Carbon and nitrogen sources that can fully grow for gluconate pyracetobacillus, utilize this fermention medium inoculation gluconate pyracetobacillus activated seed liquid, dynamic fermentation at normal temperatures, within about 8-14 days, can collect bacterial fibers crude granule, be the bacterial fibers crude granule of astaxanthin-containing.
Embodiment 2:
, a weight-reducing drinks product, it is that the bacteria cellulose uniform particles that contains astaxanthin and L-carnitine is suspended in beverage.
The preparation method of the above-mentioned bacterial fibers crude granule that contains astaxanthin and L-carnitine is as follows:
The water-soluble solution that is prepared into mass concentration 10% of L-carnitine powder (purchased from Hubei Hong Jing Chemical Co., Ltd.), natural left-handed astaxanthin emulsion (emulsification of Haematocoocus Pluvialls extract) ASTA-W1(operative norm Q/YSD03-2009 with mass concentration 1%) together with (purchased from Jingzhou City natural astaxanthin company limited), be added in fermentation medium for bacterial cellulose, making the content astaxanthin in substratum is 100ppm, and L-carnitine content is 50mg; In fermention medium, also contain the Carbon and nitrogen sources that can fully grow for gluconate pyracetobacillus, utilize this fermention medium inoculation gluconate pyracetobacillus activated seed liquid, static fermentation at normal temperatures, within about 8-14 days, on substratum upper strata, can collect bacterial cellulose wet-coating, after this wet film is ground, obtain the bacterial fibers crude granule of astaxanthin-containing and L-carnitine.
Embodiment 3:
, a whitening jelly production, it is that the bacterial fibers crude granule that contains astaxanthin and oxysuccinic acid is added in jelly production and is obtained.
The preparation method of the above-mentioned bacterial fibers crude granule that contains astaxanthin and oxysuccinic acid is as follows:
The water-soluble solution that is prepared into mass concentration 10% of oxysuccinic acid powder (purchased from Wuhan milky way industrial chemicals group), natural left-handed astaxanthin emulsion (emulsification of Haematocoocus Pluvialls extract) ASTA-W1(operative norm Q/YSD03-2009 with mass concentration 1%) together with (purchased from Jingzhou City natural astaxanthin company limited), be added in fermentation medium for bacterial cellulose, making the content astaxanthin in substratum is 50ppm, and malic acid content is 200mg; In fermention medium, also contain the Carbon and nitrogen sources that can fully grow for gluconate pyracetobacillus, utilize this fermention medium inoculation gluconate pyracetobacillus activated seed liquid, static fermentation at normal temperatures, within about 8-14 days, on substratum upper strata, can collect bacterial cellulose wet-coating, after this wet film is ground, obtain the bacterial fibers crude granule of astaxanthin-containing and oxysuccinic acid.
Embodiment 4:
, fat-reducing, a whitening nourishing drink product, it is that the bacteria cellulose uniform particles that contains astaxanthin, L-carnitine and oxysuccinic acid is suspended in beverage.
The preparation method of above-mentioned bacterial fibers crude granule is as follows:
The water-soluble solvent that is prepared into mass concentration 10% of L-carnitine powder (purchased from Hubei Hong Jing Chemical Co., Ltd.), the water-soluble solution that is prepared into mass concentration 10% of oxysuccinic acid powder (purchased from Wuhan milky way industrial chemicals group), natural left-handed astaxanthin emulsion (emulsification of Haematocoocus Pluvialls extract) ASTA-W1(operative norm Q/YSD03-2009 with mass concentration 1%) together with (purchased from Jingzhou City natural astaxanthin company limited), be added in fermentation medium for bacterial cellulose, making the content astaxanthin in substratum is 10ppm, L-carnitine content is 100mg, malic acid content is 10mg, in fermention medium, also contain the Carbon and nitrogen sources that can fully grow for gluconate pyracetobacillus, utilize this fermention medium inoculation gluconate pyracetobacillus activated seed liquid, dynamic fermentation at normal temperatures, within about 8-14 days, can collect bacterial fibers crude granule, be the bacterial fibers crude granule of astaxanthin-containing.
Above-mentioned bacterial fibers crude granule also can be added directly in cream product, makes corresponding frozen confectionery product.
The product of above-described embodiment 1-4 is offered to three groups of aspiration triers and takes, every group of 12 people, another organize 12 people take do not add astaxanthin bacterial fibers crude granule as a control group, take every night 1 time, took for 3 week, all the other diet are normal.Test-results shows, before three groups of triers' skin is compared and is taken and control group Paint Gloss flexible, before the trier who takes embodiment 2 and the product of embodiment 4 compares and takes and control group also to a certain extent body weight alleviate to some extent, average energy loss of weight 2-5%.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (2)
1. a preparation method who contains the bacteria cellulose particle product of astaxanthin, is characterized in that: the astaxanthin emulsion of mass concentration 1% is added in fermentation medium for bacterial cellulose, and making the content astaxanthin in substratum is 5-200ppm; In fermention medium, be also added with L-carnitine and oxysuccinic acid, wherein the addition of L-carnitine is that to make the content in substratum be 5-100mg; The addition of oxysuccinic acid is that to make the content in substratum be 10-500mg, recycles this fermention medium static state or dynamic fermentation and produces bacteria cellulose, then the bacterial fibers film cutting that static fermentation is obtained or be milled to particulate state; Or dynamic fermentation directly obtains bacterial fibers crude granule, finally obtain the bacteria cellulose particle product of astaxanthin-containing.
2. according to the preparation method described in claim 1, it is characterized in that: described astaxanthin is natural astaxanthin or synthesizing astaxanthin.
3. preparation method according to claim 1 and 2, is characterized in that: described fermentation, the bacterial classification of use is acetobacter xylinum.
4. preparation method according to claim 1 and 2, is characterized in that: described fermentation, the bacterial classification of use is gluconate pyracetobacillus.
5. preparation method according to claim 1 and 2, is characterized in that: in described fermention medium, contain the enough Carbon and nitrogen sources for strain growth, carbon source is glucose, fructose, sucrose and/or Sucus Cocois.
6. a bacteria cellulose particle product that contains astaxanthin, it is to be made by one of method described in claim 1-5.
7. healthcare products, is characterized in that: contain the bacteria cellulose particle product described in claim 6.
8. a nourishing drink, is characterized in that: contain bacteria cellulose particle product claimed in claim 6, bacteria cellulose uniform particles is dispersed in described beverage.
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| CN115109707A (en) * | 2022-06-10 | 2022-09-27 | 昆明理工大学 | Method for improving haematococcus pluvialis biomass and astaxanthin yield |
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| CN101979636A (en) * | 2010-11-05 | 2011-02-23 | 钟春燕 | Method for preparing spherical bacterial cellulose |
| EP2287234A1 (en) * | 2008-05-21 | 2011-02-23 | Asahi Kasei Chemicals Corporation | Cellulose powder having excellent segregation preventive effect, and compositions thereof |
| CN102127577A (en) * | 2010-12-04 | 2011-07-20 | 钟春燕 | Method for preparing bacterial cellulose gel through dynamic fermentation |
| CN102127576A (en) * | 2010-12-04 | 2011-07-20 | 钟春燕 | Colored bacteria cellulose product and preparation method thereof |
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| EP2287234A1 (en) * | 2008-05-21 | 2011-02-23 | Asahi Kasei Chemicals Corporation | Cellulose powder having excellent segregation preventive effect, and compositions thereof |
| CN101979636A (en) * | 2010-11-05 | 2011-02-23 | 钟春燕 | Method for preparing spherical bacterial cellulose |
| CN102127577A (en) * | 2010-12-04 | 2011-07-20 | 钟春燕 | Method for preparing bacterial cellulose gel through dynamic fermentation |
| CN102127576A (en) * | 2010-12-04 | 2011-07-20 | 钟春燕 | Colored bacteria cellulose product and preparation method thereof |
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