CN102524120B - Big pool simulation culturing method of US Hippocampus kelloggi larvae - Google Patents

Abstract

The invention discloses a big pool simulation culturing method of US Hippocampus kelloggi larvae. According to the big pool simulation culturing method, the US Hippocampus kelloggi larvae are transferred to an outdoor big pool from a hatching jar for large-scale culture, and the big pool simulation culturing method provides technical supports and theoretical bases for large-scale and commercial Hippocampus kelloggi culturing. With the adoption of the big pool simulation culturing method disclosed by the invention, the culturing cost is reduced by 50-60% compared with a purely indoor culturingsystem, the quality of a culturing water body is easy to control through a beneficial microorganism formulation, and high survival rate and growth rate of Hippocampus kelloggi can be guaranteed.

Description

Large pond simulation breeding method for American lined seahorse seedlings

technical field

The invention relates to the technical field of seahorse cultivation, in particular to a large pond simulation cultivation method for American lined seahorse seedlings.

Background technique

At present, there is no domestic and international patent report on large-scale cultivation of seahorse seedlings in large ponds; the traditional seahorse seedling breeding mode is temporary cultivation in hatching ponds, and the water environment of the hatching ponds has not been set up to mimic ecology, resulting in no suitable caliber of bait for the seedlings , attachable objects, beneficial water bodies or intestinal microbial populations, which largely limit the efficiency of seahorse breeding (such as low survival rate, growth rate, etc.). New Zealand seahorse biologist Woods et al. once imagined the whole process of rearing seahorse seedlings outdoors, but unfortunately, the seedlings were susceptible to death in the first 1-3 days due to environmental changes and screening of open bait. Overall, few reports have been published on the ecology of outdoor large-scale rearing of seahorse seedlings. Linedseahorse Hippocampus erectus is an Atlantic seahorse species introduced to China by Lin Qiang and Lin Junda in 2009, and has successfully bred seedlings in China; it has the characteristics of fast growth rate, strong stress resistance, and easy to change body color , making this seahorse species have great potential for market development in the world.

Contents of the invention

In order to improve the efficiency of industrialized culture of seahorse lined hippocampus seedlings, based on the existing research progress at home and abroad and the biological characteristics of lined seahorse seedlings, in this study, the seahorse seedlings were domesticated and cultured with open bait indoors for the first 5 days, and then transferred to Outdoor large-scale farming. Through the rational deployment of the simulated ecological environment, bait supply, and water quality control of the seahorse breeding pond, the goal of reducing the cost of seahorse breeding and increasing the growth rate of seahorses has been achieved.

This application technology is a breeding plan formulated according to the biological characteristics of the newly introduced American seahorse species. It has the advantages of easy operation and strong controllability, and is suitable for large-scale commercial seahorse breeding. The specific technical steps are as follows:

(1) Starvation acclimation stage after hatching of seahorse seedlings: Breed the newly hatched (1-day-old) lined seahorse seedlings in an incubation tank (0.6×0.8×1.0m), the temperature of the breeding water is 27±0.5°C, and the light intensity is 1500Lux , the light-dark cycle is 24:0, do not throw bait directly, do not add attachments, add nitrofurazone to 3-5ppm in the aquaculture water body, and soak for 5-8 hours.

(2) Opening stage of hippocampus seedlings: about 12 hours after hatching, add freshly hatched Artemia (0.21±0.04cm) and copepods (0.12±0.02cm) to the hatching tank at a density of 3-6/ ml, fed 6 times a day, fed with enough food; feces were siphoned clean every day. The light-dark cycle was adjusted to 16:8, and this process was carried out for 5 days (Note: Highly unsaturated fatty acids (SUPER Fortified for 5 hours, copepods fortified with chlorella for 2-5 days).

(3) Preparation for the large outdoor pond stage: Prepare sufficient aquaculture water for the aquaculture water in the large outdoor pond (4×6×1.5m) 3 days in advance (exposed on sunny days), sprinkle a small amount of photosynthetic bacteria, arrange green and yellow plastic artificial aquatic plants and Fresh kelp and Ulva are placed at the bottom of the large pool for seahorses to attach to. There is a small amount of air in the large pool as a whole, and the water body does not circulate.

(4) Seahorse seedling transfer and large pond culture stage: In the morning of the 5th day after hatching, take the 5-day-old hippocampus out of the hatching tank with a transparent plastic bag (or drum), and put it in the outdoor large pond for 40-60 minutes, slightly Inflated, then released from the clear plastic bag into the large pool. 5-15 day-old seahorse seedlings are mainly fed with freshly hatched Artemia and copepods; 16-40-day-old seedlings are fed with Artemia with a size of 0.37-0.52cm; 41-60-day-old seahorses are fed with a size of 0.77-0.97cm artemia and frozen mysis were fed, and the remaining frozen mysis were siphoned off every day. During the breeding process within the above 2 months, try not to change the water for the first 30-40 days, and periodically add an appropriate amount of chlorella every 3-5 days; 40-60 days, according to the quality of the water body, the overall water volume can be changed each time. 1/2 to improve the aquaculture water body.

Detailed ways

The present invention is further explained below in conjunction with the examples, but the examples do not limit the present invention in any form.

On June 5, 2009, 3 batches of newly born lined hippocampus seedlings were mixed and then sampled and divided into 3 groups (9 treatments: A1, A2, A3, B1, B2, B3 and C1, C2, C3), Among them, group A is the experimental control group, with 200 tails in each group; groups B and C are the experimental groups, and the number of hippocampi in each group is 60. The salinity, pH value and dissolved oxygen of the aquaculture water are 33±1.0‰, 7.7±0.4 and 6.5±0.5mg/L respectively, and slightly aerated to ensure that no bubbles occur.

test group

From June 5 to August 4, 2009 (60 days), there were 200 hippocampus seedlings of A1, A2 and A3 each, and the water environment was kept constant. According to the steps of this patent: firstly, the indoor hatching pond (0.6×0.8×1.0m) is raised temporarily for 5 days, the open bait is domesticated, and the strengthened bait is used for feeding. After 5 days, transfer to the prepared outdoor pond (4×6×1.5m) for breeding; 5-15-day-old seahorse seedlings are mainly fed with newly hatched Artemia and copepods; 16-40-day-old seedlings Feed with artemia with a size of 0.37-0.52cm; 41-60 day-old seahorses are fed with artemia with a size of 0.77-0.97cm and frozen mysis, and the remaining frozen mysis are siphoned clean every day. During the breeding process within 2 months, try not to change the water for the first 30-40 days, and periodically add an appropriate amount of chlorella every 3-5 days; within 40-60 days of age, the overall water volume can be changed each time according to the water quality. 1/2 improve the aquaculture water body (specifically follow the steps of this patent).

Control group 1

From June 5 to August 4, 2009 (60 days), 60 seahorse seedlings in groups B1, B2 and B3 were cultured in hatching tanks (0.6×0.8×1.0m) according to traditional methods Carrying out: temperature 27±0.5°C, light intensity 1500Lux, light-dark cycle 24:0 (after 5 days, light-dark cycle adjusted to 16:8). On day 1-5, feed the hippocampus with newly hatched artemia (0.21±0.04cm) and copepods (0.12±0.02cm) at a density of 3-6/ml, feeding 6 times a day, and feeding with full food; Feces are siphoned clean every day; 5-15 day-old seahorse seedlings are mainly fed with newly hatched Artemia and copepods; 16-40-day-old seedlings are fed with 0.37-0.52cm Artemia; 41-60 days Age seahorses were fed with artemia and frozen mysis with a size of 0.77-0.97cm, and the remaining frozen mysis were siphoned off every day.

Control group 2

Also between June 5th and August 4th, 2009 (60 days), 200 hippocampus in groups C1, C2, and C3 each. The newly hatched lined hippocampus seedlings were directly transferred to a large outdoor pond (4×6×1.5m) for breeding. The conditions during the breeding process were the same as those of the 5-day-old seahorse in the experimental group. For 1-5 days, freshly hatched seahorses were used. Artemia (0.21±0.04cm) and copepods (0.12±0.02cm) were fed to the hippocampus, fed with full food; 5-15 day old seahorse seedlings were mainly fed with newly hatched Artemia and copepods; 16-40 day-old seedlings were fed with artemia with a size of 0.37-0.52cm; 41-60-day-old seahorses were fed with artemia with a size of 0.77-0.97cm and frozen mysis, and the remaining frozen mysis were siphoned clean every day.

The results show:

Experimental group: the weight of hippocampus seedlings within 60 days was 0.87±0.11g, the body length was 5.42±0.97cm, and the survival rate was 82±3.6%.

Control group 1: hippocampus seedlings weighed 0.69±0.23g, body length was 4.35±0.52cm, and the survival rate was 76±4.2%.

Control group 2: the hippocampus seedlings had a body weight of 0.81±0.26 g, a body length of 5.83±0.76 cm, and a survival rate of 48±7.7% within 60 days.

In addition, through the analysis of water and electricity consumption, bait utilization rate, labor expenditure and other aquaculture consumption, the aquaculture cost is reduced by 50-60% compared with indoor aquaculture.

Conclusion: Combining the above three groups, the growth and survival rate of hippocampus seedlings in the experimental group has an absolute advantage, and this technology can be popularized and applied.

Claims (5)

1. the Da Chi emulation cultural method of U.S.'s strain line hippocampus seedling is characterized in that described method comprises the steps:

(1) the hippocampus seedling hatching hungry domestication stage of back: the strain line hippocampus seedling that will just hatch is cultured in the hatching cylinder;

(2) the hippocampus seedling opening stage: behind the hatching 12h, to hatching halogen worm and the copepoda that adds firm hatching in the cylinder;

(3) the outdoor Da Chi stage prepares: the breeding water body among the outdoor Da Chi was ready to sufficient breed water in advance in 3 days, whole trace inflation in the Da Chi, and water body does not circulate;

(4) the hippocampus seedling shifts and the big pool cultivated stage: hatched back 5 days, and with transparent glue bag or drum hippocampus was taken out from the hatching cylinder, be placed among the outdoor Da Chi and leave standstill, and then be discharged among the Da Chi from transparent glue bag or drum, feed.

2. according to the Da Chi emulation cultural method of the described U.S. of claim 1 strain line hippocampus seedling, it is characterized in that in the step (1), described hatching cylinder is of a size of 0.6 * 0.8 * 1.0m, the temperature of breeding water body is 27 ± 0.5 ℃, and intensity of illumination is 1500Lux, and light dark period is 24:0, not direct bait throwing in material, do not add attachment, in breeding water body, add nitrofurazone to 3～5ppm, soaked 5～8 hours.

3. according to the Da Chi emulation cultural method of the described U.S. of claim 1 strain line hippocampus seedling, it is characterized in that in the step (2), the halogen worm of described input is 0.21 ± 0.04cm, copepoda is 0.12 ± 0.02cm, described halogen worm was strengthened 5 hours with high unsaturated fatty acid, and copepoda was strengthened 2～5 days with chlorella; Density is 3～6/ml, throws something and feeds every day 6 times, is satiated with food and throws something and feeds; Ight soil siphon every day is clean, and light dark period is adjusted into 16:8, and this process will be carried out 5 days.

4. according to the Da Chi emulation cultural method of the described U.S. of claim 1 strain line hippocampus seedling, it is characterized in that in the step (3), outdoor Da Chi is of a size of 4 * 6 * 1.5m, the photosynthetic bacteria of splashing, arrange that green and the artificial pasture and water of yellow plastic and fresh and alive sea-tangle, sea lettuce adhere to supply with hippocampus in the Da Chi bottom, whole trace inflation in the Da Chi, water body does not circulate.

5. according to the Da Chi emulation cultural method of the described U.S. of claim 1 strain line hippocampus seedling, it is characterized in that in the step (4), with transparent glue bag or drum hippocampus is taken out from the hatching cylinder, be placed on and left standstill among the outdoor Da Chi 40-60 minute, slightly inflation, from transparent glue bag, be discharged into then among the Da Chi, the hippocampus seedling of 5～15 ages in days, main halogen worm and copepoda with hatching just thrown something and fed; 16～40 age in days seedling are the halogen worm nursing of 0.37～0.52cm with size; 41～60 age in days hippocampus with size be 0.77～-halogen worm and the freezing oppossum shrimp of 0.97cm throw something and feed, siphon every day of remaining freezing oppossum shrimp is clean; More than in 2 months in the breeding process, do not change water in preceding 30～40 days, and periodically added chlorella in per 3～5 days; 40～60,1/2 pair of breeding water body taking at every turn to change Total Body Water according to the water body quality improved.