CN102505357A - Electrostatic spinning melt blowing composite non-woven material for filtering blood and preparation method of electrostatic spinning melt blowing composite non-woven material - Google Patents

Abstract

The invention relates to an electrospun melt-blown composite nonwoven material for blood filtration and a preparation method thereof. The material comprises: a melt-blown receiving material and an electrospun material on the surface layer; wherein the melt-blown receiving material is a polyester slice melt-blown The electrospun material is made by drawing the fiber under the action of an electric field force and then sinking to the receiving plate covered with the meltblown material; its preparation method includes: (1) combining trifluoroacetic acid with two Methyl chloride is mixed, polyester chips are added, and spinning solution is obtained after dissolving; (2) high-voltage positive charge is applied on the needle of the syringe, and the receiving device covered with melt-blown material is grounded; the spinning solution obtained in step (1) is used for electrostatic Spinning to obtain a composite nonwoven fabric; (3) vacuum drying the above composite nonwoven fabric. The composite non-woven material of the invention has high porosity and high specific surface area, which improves the removal efficiency of leukocytes in blood; the preparation method is simple, the operation is convenient, the cost is low, and the requirements for equipment are not high.

Description

Electrospun melt-blown composite nonwoven material for blood filtration and preparation method thereof

technical field

The invention belongs to the field of composite nonwoven material and its preparation, and in particular relates to an electrospun meltblown composite nonwoven material for blood filtration and a preparation method thereof.

Background technique

During the blood transfusion in the hospital, the allogeneic reaction between the white blood cells in the donor's blood and the white blood cell antibodies in the recipient's blood will cause a variety of blood transfusion reactions. Therefore, the blood needs to be filtered before blood transfusion to remove the white blood cells. Studies have shown that compared with centrifugation, washing, and freezing to glycerolization, the filtration method has the highest removal efficiency for white blood cells, and at the same time can efficiently recover red blood cells. Therefore, the filtration method is recognized as the most economical and practical method for white blood cell filtration. .

The currently used blood filter materials are mainly polyester melt-blown materials with a fiber diameter of about 5um, a high fiber specific surface area, and good filtration and adsorption effects on white blood cells. At present, the research on blood filter materials mainly focuses on the following aspects:

1. Selection of filter substrate. After several generations of development, the blood filtration base material has gradually become finer and the pore size of the filter material has gradually become smaller. It has developed into a melt-blown filter material used at this stage. The fiber diameter is only a few microns, which greatly improves the filtration efficiency of white blood cells.

2. Polyester melt-blown nonwoven materials have poor wettability, which is not conducive to the full utilization of filter materials, affects the filtration efficiency and increases the blood filtration time, affecting the actual operation efficiency. At present, blood filtration research focuses on the exploration of surface hydrophilic modification methods of polyester melt-blown nonwoven materials. Surface grafting of hydrophilic groups and coating method are the two most important modification methods.

In recent years, electrospinning technology has been developed rapidly. It is one of the effective ways to prepare nanofibers. It provides a material with finer fibers than melt blown. The fiber diameter can even reach below 100nm. It has high porosity and low pore size. And high specific surface area and other advantages, compared with melt-blown filter materials, it has a higher filtration adsorption effect.

Contents of the invention

The technical problem to be solved by the present invention is to provide an electrospun melt-blown composite nonwoven material for blood filtration and its preparation method. The material has finer fiber fineness, smaller pore size, higher specific surface area, and higher leukocyte removal efficiency. Stable and reliable; the preparation method is simple, the operation is simple and convenient, the cost is low, and the requirements for equipment are not high.

An electrospun melt-blown composite nonwoven material for blood filtration according to the present invention, the material comprises: a melt-blown receiving material and an electrospun material located on the surface layer; wherein the melt-blown receiving material is made of polyester slices through a melt-blown process ; The electrospun material is drawn by the fiber under the action of the electric field force, and then sinks to the receiver plate covered with the grounded meltblown material.

The receiving plate is an aluminum plate.

The thickness of the melt-blown receiving material is 0.5-1.5 mm, the average fiber diameter is 1-10 um, and the material pore diameter is 1-10 um.

The thickness of the electrospun material is 0.05-0.2mm, the average fiber diameter is 100-2000nm, and the average pore diameter is 0.5-1.5um.

A method for preparing an electrospun melt-blown composite nonwoven material for blood filtration of the present invention, comprising:

(1) Preparation of spinning solution: Mix trifluoroacetic acid (TFA) and dichloromethane (DCM) at a volume ratio of 1:5-5:1, then add polyester chips, stir and dissolve to obtain a concentration of 0.1-0.3 g/mL spinning solution;

(2) Electrospinning: apply a high voltage positive charge on the needle of the syringe, and cover the receiving device of the meltblown material with grounding; use the spinning solution obtained in step (1) to carry out electrospinning, the spinning distance is 10-25cm, and the spinning The extrusion speed of the silk liquid is 0.5-2ml/h, and the spinning voltage is 15-35KV; the fibers formed by electrospinning are received on the melt-blown nonwoven fabric to obtain a composite nonwoven fabric;

(3) Vacuum drying of the composite non-woven fabric: vacuum-dry the above-mentioned composite non-woven fabric to ensure complete volatilization of the residual solvent from spinning.

The stirring described in step (1) is magnetic stirring.

The electrospinning described in step (2) was carried out under a fume hood.

The melt-blown material described in step (2) is subjected to electret treatment in advance.

In the present invention, trifluoroacetic acid and dichloromethane are used to dissolve the polyester chips; a magnetic stirrer is used to stir the spinning solution until the polymer chips are completely dissolved in the solvent to form a uniform and stable spinning solution.

In the present invention, after the spinning solution is quantitatively extruded from the syringe, it is fully drawn under the action of electric field force. During the spinning process, as the electric field force draws the spinning solution, the solvent volatilizes, and the spinning solution solidifies into fibers; the fibers formed by electrospinning are better deposited on the melt-blown cloth, and the melt-blown material can be resident in advance. extreme processing. The operation process was carried out under a fume hood to facilitate the full evaporation of solvents.

In the present invention, in order to ensure that the filter material does not pollute the blood, the electrospun melt-blown nonwoven composite material needs to be fully vacuum-dried after preparation to ensure the complete volatilization of the spinning residual solvent. Vacuum drying time is not less than 24h.

The filter material of the present invention is laminated by two components with different pore sizes to obtain a filter material with a gradient distribution of pore sizes. The melt-blown part is first in contact with blood for preliminary filtration, and the filter resistance of the melt-blown part is relatively higher than that of electrospinning. Small, the blood can fully contact with the filter material. After passing through the melt-blown layer, the fine filtration of white blood cells can be achieved through the electrospinning layer, which can significantly improve the filtration accuracy; it has great prospects in the application of blood filtration, and is of great significance for improving the removal rate of white blood cells and ensuring the health of blood transfusions.

The invention has the advantage of providing a finer filter material with higher precision for blood filtration. After the combination of electrospinning and meltblown nonwoven materials, the shortcoming of low strength of electrospinning materials is solved, and at the same time, the fiber fineness of the materials is greatly reduced. The gradient structure with pore size from large to small can also realize primary filtration to precision filtration of blood cells. The electrospun fiber reaches the nanometer level and has the characteristics of high porosity and high specific surface area. Its addition can improve the removal efficiency of white blood cells in the blood and provide a higher guarantee for the safety of blood transfusion.

Beneficial effect

(1) The electrospun melt-blown composite nonwoven material for blood filtration of the present invention has the characteristics of high porosity and high specific surface area, which improves the removal efficiency of leukocytes in blood and provides a higher guarantee for blood transfusion safety;

(2) The preparation method of the present invention is simple, easy to operate, low in cost, and does not require high equipment.

Detailed ways

Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the teachings of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

Example 1

According to the steps of preparation of the spinning solution, electrospinning and vacuum drying of the composite material included in the method for manufacturing the electrospinning melt-blown composite material for blood filtration of the present invention, the following process conditions are adopted:

(1) Polybutylene terephthalate (PBT) slices were dissolved in TFA and DCM at a volume ratio of 1:1. 2.0 g of PBT chips were weighed and dissolved in a mixture of 5 ml of TFA and 5 ml of DCM to prepare a spinning solution with a mass volume percentage of 20%. After the slices are added to the spinning solvent, a magnetic stirrer is used for stirring. During the stirring process, the solution is sealed with a parafilm, and the stirring time is 12 hours.

(2) The spinning solution is put into a syringe with a capacity of 10ml, and the distance from the spinning needle to the receiving device is 13cm; the extrusion speed of the spinning solution is 0.8ml/h. The injection needle is supplied with a high voltage of 20KV. The PBT melt-blown nonwoven material with a grammage of 105g/ ^m2 is used as the receiving material, and the spinning time is 1h.

(3) After electrospinning, put the PBT electrospun melt-blown composite material in a vacuum drying oven for 24 hours.

The average diameter of the fibers in the melt blown part is 1.82um, the average diameter of the fibers in the electrospun part is 0.59um, and the average pore diameter of the composite material is 0.79um.

Example 2

According to the steps of preparation of the spinning solution, electrospinning and vacuum drying of the composite material included in the method for manufacturing the electrospinning melt-blown composite material for blood filtration of the present invention, the following process conditions are adopted:

(1) Polybutylene terephthalate (PBT) slices were dissolved in TFA and DCM at a volume ratio of 1:1. 2.5g of PBT chips were weighed and dissolved in a mixture of 5ml of TFA and 5ml of DCM to prepare a spinning solution with a mass volume percentage of 25%. After the slices are added to the spinning solvent, a magnetic stirrer is used for stirring. During the stirring process, the solution is sealed with a parafilm, and the stirring time is 12 hours.

(2) The spinning solution is put into a syringe with a capacity of 10ml. The distance from the spinning needle to the receiving device is 13cm; the extrusion speed of the spinning solution is 1.0ml/h. The injection needle is supplied with a high voltage of 25KV. The PBT melt-blown nonwoven material with a grammage of 105g/ ^m2 is used as the receiving material, and the spinning time is 1h.

(3) After electrospinning, put the PBT electrospun melt-blown composite material in a vacuum drying oven for 24 hours.

The average diameter of the fibers in the melt blown part is 1.82um, the average diameter of the fibers in the electrospun part is 0.37um, and the average pore diameter of the composite material is 0.72um.

Example 3

According to the steps of preparation of the spinning solution, electrospinning and vacuum drying of the composite material included in the method for manufacturing the electrospinning melt-blown composite material for blood filtration of the present invention, the following process conditions are adopted:

(1) Polybutylene terephthalate (PBT) slices were dissolved in TFA and DCM at a volume ratio of 1:1. 3.0 g of PBT chips were weighed and dissolved in a mixture of 5 ml of TFA and 5 ml of DCM to prepare a spinning solution with a mass volume percentage of 30%. After the slices are added to the spinning solvent, a magnetic stirrer is used for stirring. During the stirring process, the solution is sealed with a parafilm, and the stirring time is 12 hours.

(2) The spinning solution is put into a syringe with a capacity of 10ml. The distance from the spinning needle to the receiving device is 13cm; the extrusion speed of the spinning solution is 1.2ml/h. The injection needle is supplied with a high voltage of 25KV. The PBT melt-blown nonwoven material with a grammage of 105g/ ^m2 is used as the receiving material, and the spinning time is 1h.

(3) After electrospinning, put the PBT electrospun melt-blown composite material in a vacuum drying oven for 24 hours. The average diameter of the fibers in the melt blown part is 1.82um, the average diameter of the fibers in the electrospun part is 0.85um, and the average pore diameter of the composite material is 0.89um.

Claims (8)

1. An electrospun melt-blown composite nonwoven material for blood filtration, the material comprising: a melt-blown receiving material and an electrospun material positioned on the surface; wherein the melt-blown receiving material is made of polyester slices through a melt-blown process; Electrospun materials are formed by fibers being drawn under the force of an electric field and then falling onto a grounded receiver plate covered with meltblown material. 2 . The electrospun melt-blown composite nonwoven material for blood filtration according to claim 1 , wherein the receiving plate is an aluminum plate. 3 . 3. An electrospun meltblown composite nonwoven material for blood filtration according to claim 1, characterized in that: the thickness of the meltblown receiving material is 0.5-1.5mm, and the average fiber diameter is 1-10um. The average pore size of the material is 1-10um. 4. An electrospun melt-blown composite nonwoven material for blood filtration according to claim 1, characterized in that: the thickness of the electrospun material is 0.05-0.2mm, and the average fiber diameter is 100-2000nm. The pore size is 0.5-1.5um. 5. A preparation method for electrospun melt-blown composite nonwoven material for blood filtration, comprising: (1) Mix trifluoroacetic acid and dichloromethane at a volume ratio of 1:5-5:1, then add polyester chips, stir and dissolve to obtain a spinning solution with a concentration of 0.1-0.3 g/mL; (2) Apply high voltage positive charge on the needle of the syringe, cover the receiving device of the melt blown material and ground; use the spinning solution obtained in step (1) for electrospinning, the spinning distance is 10-25cm, and the spinning solution is extruded The speed is 0.5-2ml/h, and the spinning voltage is 15-35KV; the fibers formed by electrospinning are received on the meltblown material to obtain a composite nonwoven fabric; (3) The above-mentioned composite nonwoven fabric is vacuum-dried to completely volatilize the residual solvent from spinning. 6 . The method for preparing an electrospun melt-blown composite nonwoven material for blood filtration according to claim 5 , wherein the stirring in step (1) is magnetic stirring. 6 . 7. A method for preparing an electrospun melt-blown composite nonwoven material for blood filtration according to claim 5, characterized in that: the electrospinning described in step (2) is carried out under a fume hood. 8. A method for preparing an electrospun melt-blown composite nonwoven material for blood filtration according to claim 5, characterized in that: the melt-blown material described in step (2) is subjected to electret treatment in advance.