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CN102499286B - Concentrated fermented milk used for producing lactic acid bacteria beverage and preparation method thereof - Google Patents

Concentrated fermented milk used for producing lactic acid bacteria beverage and preparation method thereof Download PDF

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CN102499286B
CN102499286B CN 201110447512 CN201110447512A CN102499286B CN 102499286 B CN102499286 B CN 102499286B CN 201110447512 CN201110447512 CN 201110447512 CN 201110447512 A CN201110447512 A CN 201110447512A CN 102499286 B CN102499286 B CN 102499286B
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程海强
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Inner Mongolia Yili Industrial Group Co Ltd
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Abstract

The invention belongs to the field of the preparation of lactic acid bacteria beverage and particularly relates to concentrated fermented milk used for producing lactic acid bacteria beverage and a preparation method thereof. The raw materials of the fermented milk comprises 12-48% by weight of skim powder, 1-3% by weight of soy protein isolate or soy peptide, 0.8-2% by weight of sea buckthorn concentrated juice and the balance of water. Compared with the prior art, the invention has the advantages that the productivity of the lactic acid bacteria beverage can be effectively improved, and particularly the productivity of the brown lactic acid bacteria beverage, which is seriously restricted due to long-term fermentation at room temperature, can be improved obviously.

Description

A kind of concentrated type acidified milk of producing sour milk beverage and preparation method thereof that is used to
Technical field
The present invention relates to the sour milk beverage preparation field, particularly, the present invention relates to a kind of concentrated type acidified milk of producing sour milk beverage and preparation method thereof that is used to.
Background technology
Active lactic acid bacteria drink stimulates intestinal movement to keeping colony balance in the human body intestinal canal, improves defecation, suppresses pernicious bacteria propagation in the enteron aisle, reduces the harmful intestinal tract material and generates, and the disorder of treatment function of intestinal canal has remarkable result.And the drinking of suitable lactose intolerance crowd.Along with the lifting day by day of present people's living standard, people begin gradually healthy living constantly to be paid attention to.Active lactic acid bacteria drink is owing to its health, palatability, and gradually growing up is the new lover in milk beverage market, and the parent who more and more is subjected to the consumer looks at.
But just the general technology of sour milk beverage production at present owing to its preparation of breast by fermentation in process of production, is carried out the allotment production of sour milk beverage again.Because the preparatory phase at acidified milk is wanted 3h-6h consuming time at least, it has limited the production production capacity of sour milk beverage to a great extent, simultaneously if will carry out long-time (48h-84h) fermentation, it has reduced the production production capacity virtually, increase the contaminated risk of fermentation, and made production cost increase etc.
Brown class active lactic acid bacteria drink is the most in fashion at present, but owing to the fermentation time of used acidified milk in this kind of beverage production is generally 48-84 hour lengthy fermentation, its production capacity is restricted to a great extent for this reason.
Sea-buckthorn is a kind of natural plants that is rich in bioactivator, and the biochemical component of having found at present reaches kind more than 190.More than the sea-buckthorn contained vitamin kind, content abundant be that any other fruits and vegetables are incomparable, have the laudatory title of " vitamin treasure-house ".Active component in the sea buckthorn fruit reaches kind more than 190, and the active component in the Seabuckthorn Oil has 106 kinds, comprising 6 kinds of liposoluble vitamins, and 22 kinds in aliphatic acid, 42 kinds of lipids, 33 kinds of flavones and phenols.It contains multiple nutritional components, mainly contains: multiple flavones, lecithin, cephalin, unrighted acid, amino acid, trace element.All contain protein in sea-buckthorn pulp, fruit juice, the seed, and content is quite high.Contain the amino acid of A wide selection of colours and designs in sea-buckthorn pulp and the fruit juice, and comprise that human body can not synthesize necessary 8 seed amino acids.
Mention soybean isolate protein powder and can effectively promote lactobacillus-fermented in the patent No. is 201010607693.2 patent, described soybean isolate protein powder has facilitation preferably to the less cultured milk's material of protein content.But in sour milk beverage is produced, the acidified milk of low protein content has limited the production capacity of sour milk beverage to a certain extent, even if Protein content then add soybean isolate protein powder during the fermentation and can not reach effect preferably in the raising acidified milk, therefore, how to make the higher milk material of protein content have good ferment effect, thereby improve acidified milk production scale, reduce cost, the preparation of leben is had great importance.
Summary of the invention
The objective of the invention is in order to overcome the problems referred to above, a kind of concentrated type acidified milk that is used to produce sour milk beverage is provided.
The objective of the invention is in order to overcome the problems referred to above, a kind of preparation method who is used to produce the concentrated type acidified milk of sour milk beverage is provided.
According to the concentrated type acidified milk that is used to produce sour milk beverage of the present invention, the weight percentage of described acidified milk raw material comprises: skimmed milk 12%-48%; Soybean protein isolate or soya-bean polypeptides 1%-3%; Sea-buckthorn inspissated juice 0.8%-2%, water surplus, as preferably, the weight percentage of described acidified milk raw material comprises: skimmed milk 12%-48%; HFCS 2%-8%; White granulated sugar 4%-10%; Soybean protein isolate or soya-bean polypeptides 1%-3%; Sea-buckthorn inspissated juice 0.8%-2%, water surplus.
According to the concentrated type acidified milk that is used to produce sour milk beverage of the present invention, described skimmed milk is meant the antibiotic-free skimmed milk, and its protein content is 33.4%-34%, and protein content is generally 26%-28% in the whole-fat milk powder.
Described HFCS can be the HFCS of F42 type or F55 type;
Described white granulated sugar is a GB primes sucrose;
Described soybean protein isolate can replace with soya-bean polypeptides, is plant separation protein matter, buys product type 670IP from Zhengzhou albumen Co., Ltd of Du Pont.
Described sea-buckthorn inspissated juice is meant fresh fructus hippophae inspissated juice, does not have any anticorrisive agent and adds.
The present invention also provides a kind of preparation method who is used to produce the concentrated type acidified milk of sour milk beverage, and the weight percentage of described acidified milk raw material comprises: skimmed milk 12%-48%; Soybean protein isolate or soya-bean polypeptides 1%-3%; Sea-buckthorn inspissated juice 0.8%-2%, water surplus;
Said method comprising the steps of:
1) water is heated up, add skimmed milk, white granulated sugar, HFCS, sea-buckthorn inspissated juice, soybean protein isolate, change material;
2) hydration, homogeneous, brown stain, cooling, inoculation, termination fermentation and homogeneous obtain being used to produce the concentrated type acidified milk of sour milk beverage.
According to a specific embodiment of the present invention, said method comprising the steps of:
(1) changes material: pure water is warming up to 55 ℃-65 ℃, drops into skimmed milk, white granulated sugar, HFCS, soybean protein isolate then, open simultaneously to stir and carry out the 30min-60minization material.
(2) hydration: change and to stop to stir after material finishes, be incubated 45 ℃-55 ℃, carry out the static hydration of 60min-120min, make abundant wetting the dissolving of skimmed milk.
(3) homogeneous: hydration is carried out homogeneous after finishing, and homogenization pressure is: 180bar/30bar is preheated to before the homogeneous about 65 ℃.
(4) brown stain: if will make that sour milk beverage is the brown color and luster, and need not mix colours, so just can carry out the browning reaction of 85 ℃ of-95 ℃/90min-120min or 121 ℃ of-132 ℃/5min-10min to the feed liquid behind the homogeneous; If do not need brown stain then directly to carry out pasteurize: 95 ℃/300s.
(5) cooling; After brown stain is finished, be cooled to 37 ℃-42 ℃ of inoculation temperatures, prepare inoculation.
(6) inoculation: the intact feed liquid of pasteurize cools, and is cooled to 37 ℃-42 ℃ of inoculation temperatures, inoculates.
(7) fermentation: fermentation time and temperature are adjusted according to selected bacterial classification.Lactobacillus casei L.casei-01, from Denmark Ke Hansen, 37 ℃ of the suitableeest fermentation temperatures.Fermentation time, according to the titratable acidity decision that acidified milk will reach, the 48h-84h that generally ferments can stop fermentation, and acidity reaches 150 ° of T-220 ° of T.
(8) stop fermentation: when acidity and pH reach product index, stop fermentation, promptly be cooled to 10 ℃-20 ℃.
(9) homogeneous: the feed liquid of fermentation ends is carried out the cold homogeneous of aseptic condition, homogenization pressure: 180bar/30bar.
(10) refrigeration: the acidified milk behind the homogeneous is refrigerated standby, reserve temperature≤10 ℃, storage time≤48h.
Be the low situation of poor efficiency, production capacity that remedies present sour milk beverage method therefor, a kind of concentrated type acidified milk that sour milk beverage is produced that is used for provided by the invention, the protein content of described concentrated type acidified milk optimization by experiment is adjusted to 4%-16%, simultaneously in the prescription of acidified milk according to a certain percentage, add soybean protein isolate and sea-buckthorn inspissated juice, effectively promote the lactic acid bacteria metabolism of acidified milk under the protein content conditions of higher, good acidified milk local flavor can be provided again simultaneously.
The present invention's benefit against existing technologies is effectively to promote the production production capacity of sour milk beverage.Particularly for the brown sour milk beverage, because its normal temperature lengthy fermentation has seriously restricted production capacity, the present invention is very much obvious to the production capacity lifting of this type of sour milk beverage.This kind is used for concentrated type acidified milk prescription and the technology that sour milk beverage is produced simultaneously, can effectively save the energy consumption in the fermenting and producing, and reduced the risk that the frequent clean sterilization does not thoroughly bring, reduced the amount of labour, and can centralized production concentrate acidified milk, carry out then cold chain be distributed to for processing factory or the allotment workshop carry out the allotment production of leben again, avoided the restriction of fermentation condition, satisfy the needs of business strategy.
Description of drawings
Fig. 1 is the preparation method's flow chart that is used to produce the concentrated type acidified milk of sour milk beverage of the present invention.
The specific embodiment
Embodiment 1
Skimmed milk 12%;
HFCS 2%;
White granulated sugar 4%;
Soybean protein isolate 1%;
Sea-buckthorn inspissated juice 0.8%;
Water surplus;
(1) changes material: pure water is warming up to 55 ℃-65 ℃, drops into skimmed milk, white granulated sugar, HFCS, soybean protein isolate then, open simultaneously to stir and carry out the 30min-60minization material.
(2) hydration: change and to stop to stir after material finishes, be incubated 45 ℃-55 ℃, carry out the static hydration of 60min-120min, make abundant wetting the dissolving of skimmed milk.
(3) homogeneous: hydration is carried out homogeneous after finishing, and homogenization pressure is: 180bar/30bar is preheated to before the homogeneous about 65 ℃.
(4) brown stain: if will make that sour milk beverage is the brown color and luster, and need not mix colours, so just can carry out the browning reaction of 85 ℃ of-95 ℃/90min-120min or 121 ℃ of-132 ℃/5min-10min to the feed liquid behind the homogeneous; If do not need brown stain then directly to carry out pasteurize: 95 ℃/300s.
(5) cooling; After brown stain is finished, be cooled to 37 ℃-42 ℃ of inoculation temperatures, prepare inoculation.
(6) inoculation: the intact feed liquid of pasteurize cools, and is cooled to 37 ℃-42 ℃ of inoculation temperatures, inoculates, and the L.casei-01 inoculum concentration is 0.01wt%-0.02wt%.
(7) fermentation: fermentation time and temperature are adjusted according to selected bacterial classification.
(8) stop fermentation: when acidity and PH reach product index, stop fermentation, promptly be cooled to 10 ℃-20 ℃.
(9) homogeneous: the feed liquid of fermentation ends is carried out the cold homogeneous of aseptic condition, homogenization pressure: 180bar/30bar.
(10) refrigeration: the acidified milk behind the homogeneous is refrigerated standby, reserve temperature≤10 ℃, storage time≤48h.
Embodiment 2
Skimmed milk 24%;
HFCS 4%;
White granulated sugar 8%;
Soybean protein isolate 2%;
Sea-buckthorn inspissated juice 1.2%;
Water surplus;
The preparation method is with embodiment 1.
Embodiment 3
Skimmed milk 48%;
HFCS 8%;
White granulated sugar 10%;
Soybean protein isolate 3%;
Sea-buckthorn inspissated juice 2%;
Water surplus;
The preparation method is with embodiment 1.
The comparative example 1
Skimmed milk 48%;
HFCS 8%;
White granulated sugar 10%;
Soybean protein isolate 3%;
Sea-buckthorn inspissated juice 0%;
Water surplus;
The preparation method is with embodiment 1.
The comparative example 2
Skimmed milk 48%;
HFCS 8%;
White granulated sugar 10%;
Soybean protein isolate 0%;
Sea-buckthorn inspissated juice 2%;
Water surplus;
The preparation method is with embodiment 1.
The comparative example 3
Skimmed milk 48%;
HFCS 8%;
White granulated sugar 10%;
Soybean protein isolate 0%;
Sea-buckthorn inspissated juice 0%;
Water surplus;
The preparation method is with embodiment 1.
Viable count variation, titratable acidity and the sense organ and the flavor evaluation that prepare product in embodiment 4 sweats
The inventor has also investigated viable count variation, titratable acidity in the sweat, and the sense organ and the flavor evaluation that prepare product, and the result is shown in table 1~3:
Titratable acidity changes in table 1 embodiment and the comparative example's sweat
Figure BDA0000126048850000061
Viable count changes in table 2 embodiment and the comparative example's sweat
Figure BDA0000126048850000062
Table 3 embodiment ferments behind the 84h, and sense organ and local flavor are tested and assessed
Embodiment Local flavor Mouthfeel Structural state
Embodiment 1 8 7 7.5
Embodiment 2 8.5 6.5 7.8
Embodiment 3 7.5 6 7
Comparative Examples 1 6.5 6 6.5
Comparative Examples 2 7 5.5 5.5
Comparative Examples 3 6.0 6.2 6.0
Annotate: in the table, the full marks of every test and appraisal are 9 minutes, and the evaluation criterion of its mouthfeel and local flavor is: flavor evaluation comprises milk fragrance, fermenting aroma two aspects, and its milk fragrance is dense more, and fermenting aroma is mellow more, and easy more being accepted do not have bad flavor, and its score is high more; Mouthfeel comprises viscosity, smooth sense two aspects, and its viscosity is moderate, and mouthfeel is smooth more, does not have granular sensation, its score is high more, and structural state comprises the quality structure of acidified milk, and it organizes fine and smooth more, does not have particle, score is high more, and is coarse more, and graininess is arranged, and then score is low more.
From above table 1, comparative example 3 and Comparative Examples 1,2,3 as can be seen, though soybean protein isolate and sea-buckthorn inspissated juice can both promote lactobacillus-fermented, along with the prolongation of fermentation time, acidity constantly increases, but required fermentation time is still very long when using separately, and with both compound according to a certain percentage interpolations, it promotes that the effect of fermentation is better, play synergy, especially when protein content was higher, its effect was better obviously, as embodiment 3 and comparative example 3.
From above table 2, comparative example 3 and Comparative Examples 1,2,3 are as can be seen, soybean protein isolate and sea-buckthorn inspissated juice can both promote the growth metabolism of lactic acid bacteria in the process of fermentation, and along with the prolongation of fermentation time, the viable lactic acid bacteria number constantly increases, with both compound according to a certain percentage interpolations, the effect of the growth metabolism of its promotion lactic acid bacteria is better, plays synergy, especially when protein content is higher, its effect is better obviously, as embodiment 3 and comparative example 3.
From above table 3, comparative example 3 and Comparative Examples 1,2,3 are as can be seen, soybean protein isolate and sea-buckthorn inspissated juice can both promote the growth metabolism of lactic acid bacteria in the process of fermentation, promote fermentation, but by compound interpolation the two, play synergy, can improve cultured milk's local flavor, mouthfeel and structural state.

Claims (2)

1. a concentrated type acidified milk that is used to produce sour milk beverage is characterized in that the weight percentage of described acidified milk raw material comprises: skimmed milk 12%-48%; Soybean protein isolate or soya-bean polypeptides 1%-3%; Sea-buckthorn inspissated juice 0.8%-2%, water surplus;
Described acidified milk raw material also comprises HFCS 2%-8%; White granulated sugar 4%-10%;
The preparation method of described concentrated type acidified milk may further comprise the steps:
1) changes material: pure water is warming up to 55 ℃-65 ℃, drops into skimmed milk, white granulated sugar, HFCS, sea-buckthorn inspissated juice, soybean protein isolate or soya-bean polypeptides then, open simultaneously to stir and carry out the 30min-60minization material;
2) hydration: change and to stop to stir after material finishes, be incubated 45 ℃-55 ℃, carry out the static hydration of 60min-120min, make abundant wetting the dissolving of skimmed milk;
3) homogeneous: hydration is carried out homogeneous after finishing, and homogenization pressure is: 180bar/30bar is preheated to 65 ℃ before the homogeneous;
4) brown stain or sterilization: the feed liquid behind the homogeneous is carried out the browning reaction of 85 ℃ of-95 ℃/90min-120min or 121 ℃ of-132 ℃/5min-10min, perhaps directly carries out 95 ℃/300s sterilization;
5) cooling: after brown stain or sterilization are finished, be cooled to 37 ℃-42 ℃ of inoculation temperatures, prepare inoculation;
6) inoculation: the inoculum concentration of Lactobacillus casei is 0.01wt%-0.02wt%;
7) fermentation: fermentation time and temperature are adjusted according to selected bacterial classification;
8) stop fermentation: when acidity and pH reach product index, stop fermentation, promptly be cooled to 10 ℃-20 ℃;
9) homogeneous: the feed liquid of fermentation ends is carried out the cold homogeneous of aseptic condition, homogenization pressure: 180bar/30bar;
10) refrigeration: the acidified milk behind the homogeneous is refrigerated standby, reserve temperature≤10 ℃, storage time≤48h.
2. one kind prepares the described method that is used to produce the concentrated type acidified milk of sour milk beverage of claim 1, it is characterized in that, said method comprising the steps of:
1) changes material: pure water is warming up to 55 ℃-65 ℃, drops into skimmed milk, white granulated sugar, HFCS, sea-buckthorn inspissated juice, soybean protein isolate or soya-bean polypeptides then, open simultaneously to stir and carry out the 30min-60minization material;
2) hydration: change and to stop to stir after material finishes, be incubated 45 ℃-55 ℃, carry out the static hydration of 60min-120min, make abundant wetting the dissolving of skimmed milk;
3) homogeneous: hydration is carried out homogeneous after finishing, and homogenization pressure is: 180bar/30bar is preheated to 65 ℃ before the homogeneous;
4) brown stain or sterilization: the feed liquid behind the homogeneous is carried out the browning reaction of 85 ℃ of-95 ℃/90min-120min or 121 ℃ of-132 ℃/5min-10min, perhaps directly carries out 95 ℃/300s sterilization;
5) cooling: after brown stain or sterilization are finished, be cooled to 37 ℃-42 ℃ of inoculation temperatures, prepare inoculation;
6) inoculation: the inoculum concentration of Lactobacillus casei is 0.01wt%-0.02wt%;
7) fermentation: fermentation time and temperature are adjusted according to selected bacterial classification;
8) stop fermentation: when acidity and pH reach product index, stop fermentation, promptly be cooled to 10 ℃-20 ℃;
9) homogeneous: the feed liquid of fermentation ends is carried out the cold homogeneous of aseptic condition, homogenization pressure: 180bar/30bar;
10) refrigeration: the acidified milk behind the homogeneous is refrigerated standby, reserve temperature≤10 ℃, storage time≤48h.
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