CN102484994B - Synergy composition of matrine and chlorantraniliprole - Google Patents

Abstract

The invention belongs to the technical field of pesticides and relates to a synergistic composition containing matrine and chlorantraniliprole. The mass ratio of matrine and chlorantraniliprole is 50:1-1:50. The matrine in the composition includes matrine, oxymatrine, and a mixture of matrine and oxymatrine, or a series of alkaloids extracted from plants such as Sophora flavescens, Sophora flavescens, and broad bean root substances and their preparations. The composition of the invention can produce synergistic effects, overcome the resistance of harmful organisms, prolong the service life of pesticides, expand the control spectrum, and be used for the control of various pests on agricultural and horticultural crops.

Description

Synergistic combination of matrine and chlorantraniliprole

technical field

The invention belongs to the technical field of pesticides, and is characterized in that it relates to a pesticide synergistic composition, in particular to the preparation and application of a synergistic composition containing matrine and chlorantraniliprole.

Background technique

The problem often faced in pest control is: how to reduce the dosage of active ingredients in pesticides to reduce or avoid adverse environmental or toxic effects, while still effectively controlling agricultural pests. Second, avoid frequent and single use of a certain pesticide variety, so as not to cause rapid selection of resistant pests or pathogens. In the pesticide compound preparation characterized by synergism, spectrum expansion, and combined treatment, insecticides with synergistic effects and different mechanisms of action are used for compounding, which not only reduces the dose of each active ingredient, but also can be used for resistance. Pest management or delaying the emergence of resistance is one of the important ways to solve the above two problems in pest control.

The plant-derived pesticide matrine (Matrine) has low toxicity to humans and animals, is safe to use, has no residue, and has stomach toxicity and contact killing effects; it directly acts on multiple targets of the worm body, paralyzes the nerve center, and then coagulates the protein of the worm body, blocking Dead pores, suffocation and death; can destroy the resistance system of pests, and have no cross-resistance with other pesticides. Matrine has an ideal control effect on vegetables Lepidoptera pests Pieris rapae, tea caterpillar, diamondback moth, piercing-sucking mouthparts aphids, tea leafhoppers, and whitefly.

Chlorantraniliprole, newly developed by DuPont, is the first o-carboxamidobenzamide insecticide, and its mechanism of action is through binding to the ryanodine receptor of the muscle cells of the pest, leading to the receptor channel Abnormal opening, calcium ions are released from the calcium pool into the cytoplasm without restriction, causing paralysis and death of pests. Due to its specific mechanism of action, there is no cross-resistance with existing insecticides, and it can effectively control pests that are resistant to existing insecticides. It is mainly stomach poisoning, with contact killing, high permeability and long-lasting effect. It has a good control effect on Lepidoptera Noctuidae, Mothidae, Fruit Mothidae, Leaf Rolleridae, Melymothidae, Plutella Plutellidae, Styracidae, Thymusidae, etc., and can also control Coleoptera phenotypes. A variety of non-lepidopteran pests such as Armidae, Chrysophyllidae, Diptera Liminidae, and Bemisia tabaci. The pesticide is of slightly toxic grade, very safe for sprayers, and also very safe for beneficial insects, fish and shrimp in paddy fields, has no residual effect on agricultural products, has good environmental compatibility, and meets the requirements of modern crop protection.

The mechanisms of action of matrine and chlorantraniliprole are completely different, and mixing them will have unpredictable results. After searching the literature, there is no report on the synergistic effect of the combination of matrine and chlorantraniliprole.

Contents of the invention

The purpose of the present invention is to make full use of plant resources, give full play to the advantages of botanical pesticides, and synergistically compound them with high-efficiency and safe chemical pesticide varieties, so as to maximize strengths and circumvent weaknesses, and provide a high-efficiency, low-toxicity, broad-spectrum and less likely to cause antibiotics. Medicinal pesticide combination - a synergistic combination of matrine and chlorantraniliprole.

The pesticide composition provided by the present invention is characterized in that: the mass ratio of the active ingredients matrine and chlorantraniliprole is 50:1 to 1:50, and the rest are pesticide adjuvants, according to methods known to those skilled in the art Processed and formulated into any formulation suitable for agricultural use in practical applications. The auxiliaries are determined according to the specific needs of the preparation.

The matrine described in the present invention includes matrine, oxymatrine, and a mixture of matrine and oxymatrine, or a series of alkaloids extracted from plants such as Sophora flavescens, Sophora flavescens, and broad bean root. substances and their preparations.

The positive effect of the present invention lies in: plant-derived pesticides and chemical pesticides are superior in promoting their strengths and avoiding their weaknesses, the mechanism of action between nerve agents and muscle agents is complementary, and the quick-acting and long-lasting properties of chlorantraniliprole make up for the slowness of matrine. Insufficient efficacy can really play the role of synergism and spectrum expansion. At the same time, it can reduce the dose of active ingredients, reduce costs, and delay the emergence of drug resistance.

Detailed ways

The synergistic effect of the composition of the present invention will be described below with an example of indoor joint toxicity test, but the present invention is not limited to the following specific test examples in practical application.

The indoor toxicity test of the synergistic composition of matrine and chlorantraniliprole in the present invention adopts conventional methods, and data calculation and processing are carried out according to the following methods:

Abbott formula was used to correct the mortality rate of each treatment, and DPS software was used to calculate LC ₅₀ and toxicity regression equation. With reference to NY/T1154.7-2006, the co-toxicity coefficient (CTC) of each mixing ratio was obtained by Sun Yunpei's method, and the synergistic effect of the mixed use of the two was evaluated.

Judgment criteria: CTC ≤ 80, showing antagonism; CTC: 80-120, showing additive effect; CTC ≥ 120, showing synergistic effect.

Test Example 1 Combined Toxicity Determination of Matrine and Chlorantraniliprole against Nilaparvata lugens

Test insect: Rice brown planthopper (Nilaparvata lugens (St l))

Test method: refer to "People's Republic of China Agricultural Industry Standard NY/T1154.11-2008" and "People's Republic of China Agricultural Industry Standard NY/T1154.7-2006" and use rice stem dipping method. Pull out the strong rice plants from the tillering stage to the early booting stage, wash them, cut them into 15 cm long rooted rice stems, and put them in a group of 3 plants, and dry them in a cool place until there is no water mark on the surface. Soak the rice stems in the diluted medicament for 30 seconds, take them out and dry them, wrap the roots with soaked absorbent cotton and put them in the culture cups, absorb the 3rd instar mid-stage nymphs and put them in the culture cups, 20 heads per cup, put They were raised in an incubator with a temperature of 27°C and a photoperiod of 16h:18h (L:D). Add water to the cotton ball wrapped with rice root every two days to keep it moist, and clean water is the control, and each concentration is repeated 3 times. After 48 hours, the number of dead insects was checked and the mortality rate was calculated.

The test results are shown in Table 1:

Table 1 Toxicity results of different ratios of matrine and chlorantraniliprole on 3rd instar nymphs of N. lugens planthopper (48h)

^* A is matrine, B is chlorantraniliprole

As can be seen from Table 1, the co-toxicity coefficient of the composition of matrine and chlorantraniliprole is greater than 120 under each ratio in the table, indicating that each ratio of matrine and chlorantraniliprole Mixing all produced synergistic effects. Among the test mixtures, the ratio of matrine: chlorantraniliprole = 20:1 had the largest co-toxicity coefficient on rice brown planthopper, with a value of 159.40.

Test Example 2 Determination of combined toxicity of matrine and chlorantraniliprole against rice leaf roller

Test insect: Cnaphalocrocis medialis (Guenee)

Test method: refer to "People's Republic of China Agricultural Industry Standard NY/T1154.7-2006". Oviposition of female moths of the rice leaf roller collected from the field. The female worms were released into a cylindrical cage, the inner wall was lined with a layer of wax paper, the outer cover was covered with black cloth, and fed with 10% honey water. The next day, the egg masses were cut out with the wax paper and placed in a petri dish to keep moist, and were reared with cultivated fresh rice leaves after hatching. The temperature of the insect culture room is (28±1)°C, and the photoperiod is 16(L):8(D). The 2nd instar larvae with uniform growth were picked out and inserted into a petri dish with a diameter of 12 cm where fresh rice leaves were placed. Put 10 rice leaf rollers into each dish, and each treatment is 5 dishes. Spray with a Potter spray tower at a pressure of 50PSI, spray one petri dish each time, spray 3 ml of liquid medicine per dish, dry the rice leaves quickly after spraying, and put them in a 25°C, 12H/12H light incubator for 48 hours Check the number of dead insects and calculate the mortality rate. The blank control was sprayed with the same amount of water. 6 concentrations were set for each treatment.

The test results are shown in Table 2:

Table 2 Toxicity results of different ratios of matrine and chlorantraniliprole on the 2nd instar larvae of the rice leaf roller

^* A is matrine, B is chlorantraniliprole

As can be seen from Table 2, the co-toxicity coefficient of the composition of matrine and chlorantraniliprole is greater than 120 to the 2nd instar larvae of the rice leaf roller under each ratio, indicating that matrine and chlorantraniliprole The mixture of each ratio produced a synergistic effect. Among the tested ratios, the ratio of matrine: chlorantraniliprole = 1:20 had the largest co-toxicity coefficient on the 2nd instar larvae of the rice leaf roller, and its value for 185.71.

Test Example 3 Combined Toxicity Determination of Matrine and Chlorantraniliprole Compound on Plutella xylostella

Test insect: diamondback moth (Plutella xylostella)

Test method: Cut fresh and clean cabbage leaves into small pieces of 3cm×4cm, put them into a petri dish with a diameter of 9cm and lined with moisturizing filter paper, spray the medicinal liquid on the leaf surface with a Potter spray tower, turn the leaves, Insert 10 3rd instar larvae for testing into each dish, and then spray the medicinal solution once, and the amount of spraying each time is 10ml. Spray the same amount of water in the same way as the blank control. After spraying, cover with gauze to prevent the larvae from escaping, and then cover the dish. Place the petri dish in an insect culture room (25+2°C), and investigate the death situation after 24 hours. 3 replicates per treatment. 10 larvae per replicate.

The test results are shown in Table 3:

Table 3 Toxicity results of different ratios of matrine and chlorantraniliprole on 3rd instar larvae of diamondback moth (24h)

^* A is matrine, B is chlorantraniliprole

As can be seen from Table 3, the co-toxicity coefficient of the composition of matrine and chlorantraniliprole is greater than 120 under each ratio, indicating that each ratio of matrine and chlorantraniliprole is mixed equally. A synergistic effect was produced, and among the tested ratios, the ratio of matrine: chlorantraniliprole = 1:10 had the largest co-toxicity coefficient on Plutella xylostella, with a value of 183.05.

Claims (4)

1. A pesticide composition, characterized in that it contains matrine and chlorantraniliprole in a synergistically effective amount, and the mass ratio of matrine and chlorantraniliprole is 50:1 to 1:50. 2. The pesticide composition according to claim 1, characterized in that matrine comprises matrine, oxymatrine and a mixture of matrine and oxymatrine, or from plant Sophora flavescens, Sophora flavescens, Guangxi A series of alkaloid extracts obtained from bean roots and their preparations. 3. Any dosage form suitable for agricultural use that the pesticide composition according to claim 1 is processed into in practical applications. 4. The application of the pesticide composition according to claim 1 in the prevention and control of agricultural and horticultural crop diseases and insect pests.