CN102478524A - Method for evaluating effectiveness of melamine test strip in dairy product detection - Google Patents
Method for evaluating effectiveness of melamine test strip in dairy product detection Download PDFInfo
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- CN102478524A CN102478524A CN2010105628485A CN201010562848A CN102478524A CN 102478524 A CN102478524 A CN 102478524A CN 2010105628485 A CN2010105628485 A CN 2010105628485A CN 201010562848 A CN201010562848 A CN 201010562848A CN 102478524 A CN102478524 A CN 102478524A
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- melamine
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- 229920000877 Melamine resin Polymers 0.000 title claims abstract description 150
- 238000012360 testing method Methods 0.000 title claims abstract description 147
- JDSHMPZPIAZGSV-UHFFFAOYSA-N melamine Chemical compound NC1=NC(N)=NC(N)=N1 JDSHMPZPIAZGSV-UHFFFAOYSA-N 0.000 title claims abstract description 145
- 238000001514 detection method Methods 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 26
- 235000013365 dairy product Nutrition 0.000 title claims abstract description 24
- 238000004519 manufacturing process Methods 0.000 claims abstract description 40
- 230000002452 interceptive effect Effects 0.000 claims abstract description 38
- 235000020185 raw untreated milk Nutrition 0.000 claims abstract description 28
- 210000000481 breast Anatomy 0.000 claims description 46
- 239000000463 material Substances 0.000 claims description 37
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 15
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 claims description 8
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 8
- 239000004202 carbamide Substances 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 8
- VGTPCRGMBIAPIM-UHFFFAOYSA-M sodium thiocyanate Chemical compound [Na+].[S-]C#N VGTPCRGMBIAPIM-UHFFFAOYSA-M 0.000 claims description 8
- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 claims description 8
- -1 microbiotic Chemical compound 0.000 claims description 6
- 150000007974 melamines Chemical class 0.000 claims description 5
- 229960002163 hydrogen peroxide Drugs 0.000 claims 1
- 235000013336 milk Nutrition 0.000 abstract 2
- 239000008267 milk Substances 0.000 abstract 2
- 210000004080 milk Anatomy 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 2
- 239000000523 sample Substances 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- GNFTZDOKVXKIBK-UHFFFAOYSA-N 3-(2-methoxyethoxy)benzohydrazide Chemical compound COCCOC1=CC=CC(C(=O)NN)=C1 GNFTZDOKVXKIBK-UHFFFAOYSA-N 0.000 description 3
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 description 3
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 239000004098 Tetracycline Substances 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 2
- 229940097572 chloromycetin Drugs 0.000 description 2
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 2
- 229910000397 disodium phosphate Inorganic materials 0.000 description 2
- 235000019800 disodium phosphate Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 241000411851 herbal medicine Species 0.000 description 2
- 229930027917 kanamycin Natural products 0.000 description 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical class O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 229960002135 sulfadimidine Drugs 0.000 description 2
- ASWVTGNCAZCNNR-UHFFFAOYSA-N sulfamethazine Chemical compound CC1=CC(C)=NC(NS(=O)(=O)C=2C=CC(N)=CC=2)=N1 ASWVTGNCAZCNNR-UHFFFAOYSA-N 0.000 description 2
- 229930101283 tetracycline Natural products 0.000 description 2
- 229960002180 tetracycline Drugs 0.000 description 2
- 235000019364 tetracycline Nutrition 0.000 description 2
- 150000003522 tetracyclines Chemical class 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 208000000913 Kidney Calculi Diseases 0.000 description 1
- 206010029148 Nephrolithiasis Diseases 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
A method for evaluating the effectiveness of a melamine test strip in dairy product detection comprises the following steps: a. obtaining the minimum concentration detection limit value of melamine in milk to be detected according to the color reaction of the melamine test strip; b. obtaining the influence of each interfering substance in the limit milk on the detection result of the melamine concentration according to the color development reaction of the melamine test strip; c. the influence of each interfering substance in the raw milk on the detection result of the melamine concentration is obtained according to the color development reaction of the melamine test strip; d. observing the detection result of each melamine test strip of the same manufacturer in the same production batch; e. observing the detection result of each melamine test strip of the same manufacturer in the same production batch; f. observing the test results of the melamine test strips of different production batches of the same manufacturer; g. and observing the test results of the melamine test strips of different production batches of the same manufacturer.
Description
Technical field
The present invention relates to a kind of method of estimating melamine test strips validity in the dairy products detection.
Background technology
Illegal retailer adds melamine in dairy products, to pretend to be protein content, adulterate, and can cause serious consequences such as kidney stone and lithangiuria to the infant.Therefore, melamine residual is the focus that attracts people's attention always in the dairy products, and the method for melamine residual occurs thereupon in the various detection dairy products, and wherein the test strips method is widely used because detection time is short, cost is low, it is low that experimenter's level is required.The producer that makes production testing dairy products melamine test strips is more and more, and the level of product is uneven, but never has a kind of method that can estimate test strips detection validity.
Summary of the invention
The purpose of this invention is to provide a kind of method of estimating melamine test strips validity in the dairy products detection, help dairy products to detect and select suitable melamine test strips.
The invention provides a kind of method of estimating melamine test strips validity in the dairy products detection, comprising:
A. adding the different melamines of measuring in the raw milk that does not contain melamine through detecting; Obtain the breast to be measured of different melamine concentration; Use the melamine test strips to detect each breast to be measured, obtain the least concentration detection limit value of Ruzhong to be measured melamine according to the chromogenic reaction of melamine test strips;
B. get many parts of raw milk; In raw milk, add melamine respectively; Preparing melamine concentration is the many parts of limit breasts that least concentration detects limit value; Add interfering material respectively to each limit Ruzhong, use the melamine test strips to detect respectively to add the limit breast behind the interfering material, obtain of the influence of each interfering material of limit Ruzhong the melamine concentration testing result according to the chromogenic reaction of melamine test strips;
C. get many parts of raw milk; In each raw milk, add interfering material respectively; Use the melamine test strips to detect respectively to add the raw milk behind the interfering material, obtain in the raw milk each interfering material to the influence of melamine concentration testing result according to the chromogenic reaction of melamine test strips;
D. get the different melamine test strips detection limit breasts of the same production batch of same manufacturer, observe the testing result of each melamine test strips of the same production batch of same manufacturer;
The different melamine test strips of e. getting the same production batch of same manufacturer detect each breast to be measured, observe the testing result of each melamine test strips of the same production batch of same manufacturer;
F. get the different melamine test strips detection limit breasts of the different production batch of same manufacturer, observe the test result of each melamine test strips of the different production batch of same manufacturer;
The different melamine test strips of g. getting the different production batch of same manufacturer detect each breast to be measured, observe the test result of each melamine test strips of the different production batch of same manufacturer.
A kind of estimate dairy products detect in another schematic embodiment of method of melamine test strips validity, in a step, the concentration of Ruzhong to be measured melamine is respectively 0,150,300,600,1500 and 3000ug/kg.
A kind of estimate dairy products detect in the another kind of schematically embodiment of method of melamine test strips validity; In b and the c step, interfering material is melamine, hydrogen peroxide, microbiotic, L-hydroxyproline, urea, separates anti-agent and sodium thiocyanate.
A kind of estimate dairy products detect in another schematic embodiment of method of melamine test strips validity, in d and the f step, carry out 10 repeated tests with melamine test strips detection limit breast.
A kind of estimate dairy products detect in another schematic embodiment of method of melamine test strips validity, in f and the g step, production batch be respectively same day date of manufacture batch, 1/2 shelf-life batch and same day date shelf-life batch.
A kind of method of estimating melamine test strips validity in the dairy products detection has following characteristics:
1. conceive rigorous.
2. lack detection time, contain the sample preparation time, amount to 20 minutes.
3. detect and need not additional instruments equipment, detection method is simple.
4. the result judges easily.
Embodiment
A kind of method of estimating melamine test strips validity in the dairy products detection comprises:
A. adding the different melamines of measuring in the raw milk that does not contain melamine through detecting; Obtain the breast to be measured of different melamine concentration; Use the melamine test strips to detect each breast to be measured, obtain the least concentration detection limit value of Ruzhong to be measured melamine according to the chromogenic reaction of melamine test strips;
B. get many parts of raw milk; In raw milk, add melamine respectively; Preparing melamine concentration is the many parts of limit breasts that least concentration detects limit value; Add interfering material respectively to each limit Ruzhong, use the melamine test strips to detect respectively to add the limit breast behind the interfering material, obtain of the influence of each interfering material of limit Ruzhong the melamine concentration testing result according to the chromogenic reaction of melamine test strips;
C. get many parts of raw milk; In each raw milk, add interfering material respectively; Use the melamine test strips to detect respectively to add the raw milk behind the interfering material, obtain in the raw milk each interfering material to the influence of melamine concentration testing result according to the chromogenic reaction of melamine test strips;
D. get the different melamine test strips detection limit breasts of the same production batch of same manufacturer, observe the testing result of each melamine test strips of the same production batch of same manufacturer;
The different melamine test strips of e. getting the same production batch of same manufacturer detect each breast to be measured, observe the testing result of each melamine test strips of the same production batch of same manufacturer;
F. get the different melamine test strips detection limit breasts of the different production batch of same manufacturer, observe the test result of each melamine test strips of the different production batch of same manufacturer;
The different melamine test strips of g. getting the different production batch of same manufacturer detect each breast to be measured, observe the test result of each melamine test strips of the different production batch of same manufacturer.
Step a sensitivity (detection limit) test
Filter out the raw milk that does not contain melamine through the ELISA detection.According in No. the 235th, the Ministry of Agriculture bulletin " animal food herbal medicine MRL " for the high-load requirement-300ug/kg of melamine in the dairy produce; In raw milk, add the melamines of different amounts, obtain melamine concentration and be respectively 0,150,300,600,1500 and the breast to be measured of 3000ug/kg.
Detect step
1. draw 1ml breast to be measured in the 2ml centrifuge tube, centrifugal 10 minutes of 2000G removes upper strata fat, obtains handling breast;
2. draw and handle newborn 100ul in the 2ml centrifuge tube; Add 100ul sample diluting liquid (PBS damping fluid, the concrete prescription as follows: potassium dihydrogen phosphate (KH2PO4) 0.24 gram, sodium hydrogen phosphate (Na2HPO4) 3.63 grams again; Sodium chloride (NaCl) 8 grams; Potassium chloride (KCl) 0.2 gram adds the water constant volume to 1L) in centrifuge tube, mixing obtains the dilution breast;
3. draw 100ul dilution breast and add in the test strips well 5-10 minute sentence read result.
The result judges
When reading as a result, cannot or be inverted test strips from the test strips side and carry out observation as a result.Testing result is divided into:
Negative: T line (detection line is near an end that splashes into sample solution) is judged as feminine gender than C line (control line) deeply or equally dark, and the melamine drug concentration is lower than the test paper LDL in the expression sample.
Positive: the T line is more shallow than C line, or the T line do not have colour developing, is judged as the positive, and melamine concentration is higher than the test paper LDL in the expression sample;
Invalid: the C line does not develop the color, and shows that incorrect operating process or detector bar lost efficacy.In the case, using new detector bar tests again.
Testing result
Respectively to be respectively 0,150,300,600,1500 and the dilution breast test that makes of the breast to be measured of 3000ug/kg by melamine concentration; (content in the dilution Ruzhong is 150ug/kg) was turning point when discovery was 300ug/kg corresponding to the content of melamine in Ruzhong to be measured; The least concentration that is melamine detects limit value; During greater than this concentration; T line on the melamine test strips is all shallow or colourless than C line, and the least concentration detection limit value that obtains Ruzhong to be measured melamine thus is 300ug/kg, satisfies the requirement of No. 235 bulletin of the Ministry of Agriculture " animal food herbal medicine MRL " to the content of melamine measurement sensitivity.If the melamine test strips is that 300ug/kg is still real negative at content of melamine, explain that the melamine test strips sensitivity of this type is not enough.
Following step b and step c relate to the specificity test in the melamine test strips validity.
Step b
Get raw milk; In raw milk, add melamine; Preparing melamine concentration is the many parts of limit breasts of 300ug/kg, adds interfering material respectively to many parts of limit Ruzhongs, and these interfering materials are melamine, hydrogen peroxide, microbiotic, L-hydroxyproline, urea, separate anti-agent and sodium thiocyanate; Wherein, the concentration of each these interfering material of limit Ruzhong is respectively:
Hydrogen peroxide: 30mg/kg;
Microbiotic: penicillin 4ug/kg+ tetracycline 100ug/kg+ sulfamethazine 100ug/kg+kanamycins 150ug/kg+ chloromycetin 0.3ug/kg+ streptomysin 200ug/kg (above antibiotic content all meets or exceeds limiting the quantity of of national Specification);
L-hydroxyproline: 10 mg/kg;
Urea: 500 mg/kg;
Separate anti-agent: 4U/ml;
Sodium thiocyanate: 0.5 mg/kg.
Choosing all of above interfering material concentration with reference to concerned countries standard pair regulation with these interfering materials concentration content limit value in dairy products.
The result judges with the deterministic process as a result among the above-mentioned step a.
Testing result
The limit Ruzhong of contain hydrogen peroxide, microbiotic, L-hydroxyproline, urea respectively, separating anti-agent and sodium thiocyanate; The display result of melamine test strips is all positive; Explain that limit Ruzhong interfering material does not interfere reaction and changes test result with the melamine test strips, this kind melamine test strips is reliable; If feminine gender appears in the real result of melamine test strips, explain and disturb the Ruzhong interfering material and interfere reaction and change test result that this kind melamine test strips is unreliable with the melamine test strips.
Step c
Get many parts of raw milk; In each part raw milk, add interfering material respectively; These interfering materials are hydrogen peroxide, microbiotic, L-hydroxyproline, urea, separate anti-agent and sodium thiocyanate (these interfering materials are modal interpolation materials in the present dairy products); Prepare and disturb breast (each disturbs the Ruzhong only to contain a kind of interfering material), wherein, each disturbs the concentration of the above-mentioned interfering material in Ruzhong to be:
Hydrogen peroxide: 30mg/kg;
Microbiotic: penicillin 4ug/kg+ tetracycline 100ug/kg+ sulfamethazine 100ug/kg+kanamycins 150ug/kg+ chloromycetin 0.3ug/kg+ streptomysin 200ug/kg (above antibiotic content all meets or exceeds limiting the quantity of of national Specification);
L-hydroxyproline: 10 mg/kg;
Urea: 500 mg/kg;
Separate anti-agent: 4U/ml;
Sodium thiocyanate: 0.5 mg/kg.
Choosing all of above interfering material concentration with reference to concerned countries standard pair regulation with these interfering materials concentration content limit value in dairy products.
The result judges with the deterministic process as a result among the above-mentioned step a.
Testing result
The interference Ruzhong of contain hydrogen peroxide, microbiotic, L-hydroxyproline, urea respectively, separating anti-agent and sodium thiocyanate; The display result of melamine test strips is all positive; Explain and disturb the Ruzhong interfering material not interfere reaction and change test result with the melamine test strips, this kind melamine test strips is reliable; If feminine gender appears in the real result of melamine test strips, explain and disturb the Ruzhong interfering material and interfere reaction and change test result that this kind melamine test strips is unreliable with the melamine test strips.
Following steps d, e, f and g relate to the stability test in the melamine test strips validity.Wherein steps d and e confirm batch internal stability; Stability between step f and g confirm to criticize.
Steps d is used same batch melamine test strips of same manufacturers produce; To same melamine concentration is that the limit breast of 300ug/kg carries out 10 experiments repeatedly; Promptly get same batch melamine test strips of 10 same manufacturers produce, detecting same melamine concentration respectively is the limit breast of 300ug/kg and the color of observing test strips.
Step e uses same batch melamine test strips of same manufacturer production, and each melamine concentration breast to be measured among the step a is done parallel laboratory test, and promptly the breast to be measured to same concentration level uses different test strips repeated detection and the color of observing it.
The result judges with the deterministic process as a result among the above-mentioned step a.
Testing result
If 10 times of steps d are detected the test result of limit breast all positive; And the test result of breast to be measured on same concentration level for different melamine concentration among the step e is consistent, and then the consistance in the same production batch of melamine test strips is qualified.
Step f uses the melamine test strips of the different batches of same manufacturer production; For example same day date of manufacture batch; 1/2 shelf-life batch with same day date shelf-life batch be that the limit breast of 300ug/kg carries out 10 experiments repeatedly to same melamine concentration; Promptly get the melamine test strips of the different batches of 10 same manufacturers produce, detecting same melamine concentration respectively is the limit breast of 300ug/kg and the color of observing test strips.
Step g is used the melamine test strips of the different batches of same manufacturer production; For example same day date of manufacture batch; 1/2 shelf-life batch with same day date shelf-life batch; Each melamine concentration breast to be measured among the step a is done parallel testing experiment, and the test strips of promptly using different batches is to the breast to be measured of same concentration level repeated detection and observe the color of test strips respectively.
The result judges with the deterministic process as a result among the above-mentioned step a.
Testing result
If the test result of step f is all positive, and the test result of breast to be measured on same concentration level for different melamine concentration is consistent in the step g, and then the consistance between the production batch of melamine test strips is qualified.
A kind of method of estimating melamine test strips validity in the dairy products detection has following characteristics:
1. conceive rigorous.
2. lack detection time, contain the sample preparation time, amount to 20 minutes.
3. detect and need not additional instruments equipment, detection method is simple.
4. the result judges easily.
Therefore, this melamine fast detecting test paper bar meets dairy enterprises and detects the melamine needs, can use.
The listed a series of detailed description of preceding text only is specifying to feasibility embodiment of the present invention; They are not in order to restriction protection scope of the present invention, allly do not break away from equivalent embodiment or the change that skill of the present invention spirit done and all should be included within protection scope of the present invention.
Claims (5)
- One kind estimate dairy products detect in the method for melamine test strips validity, comprising:A. adding the different melamines of measuring in the raw milk that does not contain melamine through detecting; Obtain the breast to be measured of different melamine concentration; Use the melamine test strips to detect each said breast to be measured, obtain the least concentration detection limit value of said Ruzhong to be measured melamine according to the chromogenic reaction of said melamine test strips;B. get many parts of said raw milk; In said raw milk, add melamine respectively; Preparing melamine concentration is the many parts of limit breasts that said least concentration detects limit value; Add interfering material respectively to each said limit Ruzhong, use said melamine test strips to detect respectively to add the said limit breast behind the said interfering material, obtain of the influence of each said interfering material of said limit Ruzhong the melamine concentration testing result according to the chromogenic reaction of said melamine test strips;C. get many parts of said raw milk; In each said raw milk, add interfering material respectively; Use said melamine test strips to detect respectively to add the said raw milk behind the said interfering material, obtain in the said raw milk each said interfering material to the influence of melamine concentration testing result according to the chromogenic reaction of said melamine test strips;The different said melamine test strips of d. getting the same production batch of same manufacturer detects said limit breast, observes the testing result of each said melamine test strips of the same production batch of same manufacturer;The different said melamine test strips of e. getting the same production batch of same manufacturer detects each said breast to be measured, observes the testing result of each said melamine test strips of the same production batch of same manufacturer;The different said melamine test strips of f. getting the different production batch of same manufacturer detects said limit breast, observes the test result of each said melamine test strips of the different production batch of same manufacturer;The different said melamine test strips of g. getting the different production batch of same manufacturer detects each said breast to be measured, observes the test result of each said melamine test strips of the different production batch of same manufacturer.
- 2. the method for claim 1, among the wherein said step a, the concentration of said Ruzhong to be measured melamine is respectively 0,150,300,600,1500 and 3000ug/kg.
- 3. the method for claim 1, wherein in b and the c step, said interfering material is oxydol, microbiotic, L-hydroxyproline, urea, separates anti-agent and sodium thiocyanate.
- 4. the method shown in claim 1 wherein in d and the f step, detects said limit breast with said melamine test strips and carries out 10 repeated tests.
- 5. the method shown in claim 1, wherein in f and the g step, said production batch be respectively same day date of manufacture batch, 1/2 shelf-life batch with same day date shelf-life batch.
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| CN2010105628485A CN102478524A (en) | 2010-11-29 | 2010-11-29 | Method for evaluating effectiveness of melamine test strip in dairy product detection |
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| CN2010105628485A CN102478524A (en) | 2010-11-29 | 2010-11-29 | Method for evaluating effectiveness of melamine test strip in dairy product detection |
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2010
- 2010-11-29 CN CN2010105628485A patent/CN102478524A/en active Pending
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| CN101329280A (en) * | 2008-07-31 | 2008-12-24 | 内蒙古蒙牛乳业（集团）股份有限公司 | Fast testing method of hydrogen peroxide residue in milk-like liquid and testing reagent kit thereof |
| US20100112604A1 (en) * | 2008-11-04 | 2010-05-06 | Joy Ann Drawdy | Home food test kit and method of use |
| WO2010090751A2 (en) * | 2009-02-05 | 2010-08-12 | The Regents Of The University Of California | Methods and materials for the detection of melamine |
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