CN102477419B - Method for in-vitro fusion of stem cells and porous biomaterial - Google Patents
Method for in-vitro fusion of stem cells and porous biomaterial Download PDFInfo
- Publication number
- CN102477419B CN102477419B CN201010560340.1A CN201010560340A CN102477419B CN 102477419 B CN102477419 B CN 102477419B CN 201010560340 A CN201010560340 A CN 201010560340A CN 102477419 B CN102477419 B CN 102477419B
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- porous biomaterial
- stem cell
- negative pressure
- porous
- fusion
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- 239000012620 biological material Substances 0.000 title claims abstract description 30
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 18
- 230000004927 fusion Effects 0.000 title claims abstract description 12
- 238000000338 in vitro Methods 0.000 title claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 28
- 239000006285 cell suspension Substances 0.000 claims abstract description 16
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000002985 plastic film Substances 0.000 claims abstract description 4
- 229920006255 plastic film Polymers 0.000 claims abstract description 4
- 239000000741 silica gel Substances 0.000 claims abstract description 4
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 4
- 210000004027 cell Anatomy 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 230000007910 cell fusion Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000005253 cladding Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003566 sealing material Substances 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Materials For Medical Uses (AREA)
Abstract
A method for in-vitro fusion of stem cells and a porous biomaterial includes the steps: firstly, enabling the porous biomaterial (2) to be hermetic by the aid of a hermetic material (1), and enabling one end (3a) of each of two pipelines (3) to be hermetic in the porous biomaterial (2) in the hermetic process; secondly, connecting an input device (4) with the other end (3b) of one pipeline (3); thirdly, connecting a negative pressure device (5) with the other end (3b) of the other pipeline (3); and fourthly, utilizing the input device (4) to push in stem cell suspension while enabling one end of the porous biomaterial to be in the negative pressure state by the aid of the negative pressure device (5), so that the stem cell suspension completely reaches the inside of the porous biomaterial for fusion. The hermetic material (1) is soft silica gel or a sticky plastic film. The method has the advantages of capability of effectively realizing fusion of crisp porous biomaterials in various shapes and stem cells, quickness, high efficiency, simplicity in processing and capability of being widely applied to scientific research and clinical application.
Description
Technical field
The present invention relates to stem cell experiment and Application Areas, the specifically external fusing device of a kind of stem cell and porous biomaterial.
Background technology
For the fixing high biomaterial of proterties and fragility, prior art mainly drops in stem cell suspension each surface of material and reaches the compound of stem cell and material; For the closed unit of granular porous material application solid shape by stem cell suspension by material by cell and Material cladding.
Prior art mainly contains following shortcoming: 1. cell suspension is splashed into material surface, due to after surface void sealed by cell, material inside can not reach effective cell concentration, when especially material is larger; By cell suspension when being placed in the material of closed unit of solid shape, because the gap of material and closed unit wall easily makes suspension pass through gap but not through material, and this device is not suitable for non-particulate shape and the large material of fragility.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of and stem cell effectively can be compound to the surface of porous biomaterial and inner method for in-vitro fusion.
In order to address the above problem technical scheme of the present invention, be such:
Stem cell and a porous biomaterial method for in-vitro fusion, is characterized in that, the steps include:
1. with enclosed material, porous biomaterial is airtight, in airtight process, one end of two pipelines is enclosed in porous biomaterial;
2. input unit is connected with the other end of a pipeline;
3. negative pressure device is connected with the other end of another root pipeline;
4. use input unit to push stem cell suspension, use negative pressure device to make porous biomaterial in one end negative pressure state simultaneously, make stem cell suspension arrive material internal completely and merge;
Described enclosed material is soft silica gel or viscous plastic film.
Described input unit is ordinary syringe.
Described negative pressure device is ordinary syringe or vacuum pump.
Positively effect of the present invention is: apply specific closed material and guarantee the effectively difform biological stephanoporate material of sealing, at one end in the situation of negative pressure, stem cell suspension is pushed, thereby guarantee that suspension can be effectively inner through biomaterial, can effectively solve biological stephanoporate material that different shape, quality are crisp and the problem of stem cell fusion, method is quick, efficiently, simple to operate, can be widely used in scientific research and clinical application.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, describe the present invention in detail:
Fig. 1 is the stereographic map of embodiment 1.
Number in the figure is: 1-enclosed material, 2-porous biomaterial, 3-pipeline, 4-input unit, 5-negative pressure device.
Embodiment
For technique means, creation characteristic that the present invention is realized, reach object and effect is easy to understand, below in conjunction with concrete diagram, further set forth the present invention.
embodiment 1
Stem cell and a porous biomaterial method for in-vitro fusion, step is:
1. use enclosed material 1 that porous biomaterial 2 is airtight, in airtight process, one end 3a of two pipelines 3 is enclosed in porous biomaterial 2, described sealing material 1 is soft silica gel or viscous plastic film;
2. input unit 4 is connected with the other end 3b of pipeline 3, as shown in Figure 1, described input unit 4 is ordinary syringe, before connecting, in syringe, should pack stem cell suspension in advance;
3. negative pressure device 5 is connected with the other end 3b of pipeline 3, as shown in Figure 1, described negative pressure device 5 is ordinary syringe, before being connected with pipeline 3 syringe planted agent in vacuum state, with one end of guaranteeing porous material in negative pressure state;
4. apply input unit 4 and push stem cell suspension, pull the piston of negative pressure device 5 simultaneously, make one end of porous biomaterial always in negative pressure state, make stem cell suspension arrive material internal completely and merge.
embodiment 2
When porous biomaterial volume is larger, when syringe cannot meet required stem cell suspension amount, negative pressure device 5 can adopt vacuum pump.
Positively effect of the present invention is: apply specific closed material and guarantee the effectively difform biological stephanoporate material of sealing, at one end in the situation of negative pressure, stem cell suspension is pushed, thereby guarantee that suspension can be effectively inner through biomaterial, can effectively solve biological stephanoporate material that different shape, quality are crisp and the problem of stem cell fusion, method is quick, efficiently, simple to operate, can be widely used in scientific research and clinical application.
Claims (4)
1. stem cell and a porous biomaterial method for in-vitro fusion, is characterized in that, the steps include:
1. use enclosed material (1) that porous biomaterial (2) is airtight, in airtight process, one end (3a) of two pipelines (3) is enclosed in porous biomaterial (2);
2. input unit (4) is connected with the other end (3b) of a pipeline (3);
3. negative pressure device (5) is connected with the other end (3b) of another root pipeline (3);
4. use input unit (4) to push stem cell suspension, use negative pressure device (5) to make porous biomaterial in one end negative pressure state simultaneously, make stem cell suspension arrive material internal completely and merge;
Described enclosed material is soft silica gel or viscous plastic film.
2. stem cell according to claim 1 and porous biomaterial method for in-vitro fusion, is characterized in that, described input unit (4) is ordinary syringe.
3. stem cell according to claim 1 and porous biomaterial method for in-vitro fusion, is characterized in that, described negative pressure device (5) is ordinary syringe.
4. stem cell according to claim 1 and porous biomaterial method for in-vitro fusion, is characterized in that, described negative pressure device (5) is vacuum pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010560340.1A CN102477419B (en) | 2010-11-26 | 2010-11-26 | Method for in-vitro fusion of stem cells and porous biomaterial |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010560340.1A CN102477419B (en) | 2010-11-26 | 2010-11-26 | Method for in-vitro fusion of stem cells and porous biomaterial |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102477419A CN102477419A (en) | 2012-05-30 |
| CN102477419B true CN102477419B (en) | 2014-04-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010560340.1A Active CN102477419B (en) | 2010-11-26 | 2010-11-26 | Method for in-vitro fusion of stem cells and porous biomaterial |
Country Status (1)
| Country | Link |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998000174A2 (en) * | 1996-07-03 | 1998-01-08 | Cleveland Clinic Foundation | Method of preparing a composite bone graft |
| CN1565643A (en) * | 2003-07-03 | 2005-01-19 | 中国人民解放军军事医学科学院基础医学研究所 | Tissue engineered cartilage based on bone marrow mesenchymal stem cell |
| CN1746295A (en) * | 2004-09-09 | 2006-03-15 | 中国人民解放军军事医学科学院基础医学研究所 | A kind of tissue engineered cartilage based on placenta-derived mesenchymal stem cells and its manufacturing method |
| CN101332135A (en) * | 2007-06-27 | 2008-12-31 | 德普伊产品公司 | Osteogenic prostheses, related appliance and method |
-
2010
- 2010-11-26 CN CN201010560340.1A patent/CN102477419B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998000174A2 (en) * | 1996-07-03 | 1998-01-08 | Cleveland Clinic Foundation | Method of preparing a composite bone graft |
| CN1565643A (en) * | 2003-07-03 | 2005-01-19 | 中国人民解放军军事医学科学院基础医学研究所 | Tissue engineered cartilage based on bone marrow mesenchymal stem cell |
| CN1746295A (en) * | 2004-09-09 | 2006-03-15 | 中国人民解放军军事医学科学院基础医学研究所 | A kind of tissue engineered cartilage based on placenta-derived mesenchymal stem cells and its manufacturing method |
| CN101332135A (en) * | 2007-06-27 | 2008-12-31 | 德普伊产品公司 | Osteogenic prostheses, related appliance and method |
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| Publication number | Publication date |
|---|---|
| CN102477419A (en) | 2012-05-30 |
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Application publication date: 20120530 Assignee: Abbott (Beijing) Medical Technology Co.,Ltd. Assignor: SHANGHAI NINTH PEOPLE'S HOSPITAL SHANGHAI JIAOTONG University SCHOOL OF MEDICINE Contract record no.: X2022310000058 Denomination of invention: An in vitro fusion method of stem cells and porous biomaterials Granted publication date: 20140423 License type: Exclusive License Record date: 20220809 |
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