CN102465089B - Method for culturing photosynthetic microorganism and photoreactor used in same - Google Patents
Method for culturing photosynthetic microorganism and photoreactor used in same Download PDFInfo
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- CN102465089B CN102465089B CN 201110263961 CN201110263961A CN102465089B CN 102465089 B CN102465089 B CN 102465089B CN 201110263961 CN201110263961 CN 201110263961 CN 201110263961 A CN201110263961 A CN 201110263961A CN 102465089 B CN102465089 B CN 102465089B
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Abstract
The invention discloses a photoreactor, comprising a photoreactor body, a rotatable mechanical shaft and a supporting base. The photoreactor is characterized in that the photoreactor body comprises two light-receiving panels and borders; the bottom border of the photoreactor body is connected with the supporting base through the rotatable mechanical shaft; and a long clapboard and a plurality of short clapboards are arranged in parallel inside the photoreactor body, a photoreaction zone is laterally divided into a liquid ascending region and a liquid descending region by the long clapboard, and a gas distributor is arranged at the bottom of the liquid ascending region and is connected with an external gas inlet pipeline through a gas inlet. The photoreactor disclosed by the invention has the advantages of large reaction specific surface area, good air permeability, and good performance in mixing and circulating a culture solution, and is suitable for the large-scale culture of photosynthetic energy microorganisms.
Description
Technical field
The present invention relates to a kind of photoreactor and method of culturing micro-organisms, relate in particular to the bioreactor that is used for the photosynthetic microorganism cultivation that a kind of inside has the diaphragm structure setting of different lengths.
Background technology
Photoreactor is applied to the suitability for industrialized production that algae, photosynthetic bacterium, mosses or other plant or microorganism cells are cultivated usually.These plants or microorganism, particularly photosynthetic microorganism can utilize luminous energy with CO
2Be converted into various organism with water, have that photosynthetic efficiency height, environmental compatibility are strong, breeding is fast, growth cycle lack, do not occupy cultivated land, can change on a large scale, automatization control cultivation characteristics such as (productions).
Current, the cultivation of photosynthetic microorganism mainly contains open and closed two kinds of bioreactors, and open bioreactor is not owing to possess temperature control capability, produce and to be subjected to seasonal effect, and production process is subjected to the pollution of various heteroplasia things easily.The main base form of closed photo bioreactor has duct type, column formula, flat etc.These photoreactors need satisfy following requirement: 1) make the maximum luminous energy of each cell acceptance in the reactor.2) mix fully and strengthen gaseous interchange.Duct type bioreactor specific surface area is big, but oxygen is resolved difficulty, and the while is easy cleaning not, is not suitable for the large scale culturing of photosynthetic microorganism.Though the column bioreactor does not exist oxygen to resolve the problem of difficulty, mixed solution is even, amplifies difficulty.Flat bioreactor is for the two kinds of photoreactors in front, have simultaneously that the light application ratio surface-area is big, illumination utilization ratio height, mix, advantages such as relatively easy are resolved soon, amplified to oxygen, therefore has certain advantage with flat bioreactor as foundation structure, than the plant-scale application of more promising, the easier realization of the closed photoreactor of other types.
And in existing flat bioreactor, usually the diaphragm structure that adopts all is the equal length dividing plate that is crisscross arranged, this set makes that the reaction between feed liquid and the gas is abundant inadequately, simultaneously, processing to discarded feed liquid is convenient inadequately, and install the not shortcoming of easy cleaning, simultaneously, the flow of gas and the circulation of feed liquid are because the not convenient unpredictable situations of handling such as obstruction that also may cause device, therefore, demand providing a kind of photoreactor that can overcome the various defectives that exist in above-mentioned existing manufacturing technology and the technology urgently.
Summary of the invention
The invention provides a kind of photoreactor for the photosynthetic microorganism cultivation.Reactor light application ratio surface-area of the present invention is big, illumination utilization ratio height, photosynthetic microorganism and nutriment mix, and that oxygen is resolved is fast, be easy to handle discarded feed liquid, cleans easily, does not cause the obstruction of device.
In the photoreactor of the present invention:
Described photoreactor comprises the photoreactor main body, and rotating mechanical axis and base for supporting is characterized in that:
Described photoreactor main body is flats cubes enclosed construction, be connected with external system with material import and export by gas inlet and outlet, described photoreactor main body is by two sensitive surface panels, top frame and bottom frame, and left border and Right Border composition, described top frame, bottom frame, left border and Right Border are used for fixing two sensitive surfaces;
The bottom frame of described photoreactor main body is connected with described base for supporting by described rotating mechanical axis;
Have parallel long dividing plate and a plurality of breviseptum that arranges in the photoreactor body interior, described long dividing plate closely is connected with described two sensitive surface panels with breviseptum; Described long dividing plate one end is connected with described bottom frame, the other end is not connected with the top frame, the breviseptum two ends all are not connected with the top frame with the bottom frame, and long dividing plate is littler than the distance of breviseptum and top frame with the distance of top frame, and namely long dividing plate is higher than breviseptum;
Be divided into photoresponse district and plenum zone in the photoreactor main body up and down, in addition, described photoresponse district is divided into liquid elevated areas and the liquid zone that descends about by long dividing plate, and breviseptum is positioned at the liquid elevated areas and with the parallel distribution of identical spacing;
Bottom in described liquid elevated areas is provided with gas distributor, and described gas distributor is connected with the external feed stream pipeline by inlet mouth;
Described inlet mouth is positioned on the frame of described photoreactor liquid elevated areas one side, and the air outlet is positioned on the top frame of photoreactor main body; Preferably, described inlet mouth is tubular structure;
Opening for feed and inlet mouth homonymy arrange, and discharge port is positioned at liquid and descends on the frame or underframe of a regional side.
In photoreactor of the present invention, the nutrient solution that is used for cultivation photosynthetic microorganism or other microorganism or plant enters the photoreactor main body by opening for feed, rise from the photoreactor bottom part body in the liquid elevated areas, the influent zone that descends when being higher than long dividing plate, and flow out by discharge port.The photosynthetic microorganism of cultivating or other microorganism or plant breed in nutrient solution and photoresponse, so nutrient solution rests on the photoresponse district that the interior part of reactor namely constitutes described photoreactor main body.The gas that the external feed stream pipeline provides enters the photoresponse district by gas distributor, and the rising of nutrient solution nutrient solution and release through the liquid elevated areas enter the headspace of described photoreactor main body, i.e. plenum zone.
According to a preferred embodiment of the present invention, described liquid elevated areas has 8-20 bar breviseptum.
According to a preferred embodiment of the present invention, described photoreactor also comprises angle regulator, and this angle regulator is connected respectively with base for supporting with the photoreactor main body, and the photoreactor main body can be rotated around described mechanical axis by described angle regulator.
According to another preferred embodiment of the present invention, gas distributor described in the wherein said photoreactor is for extending to the diffuser that approaches long dividing plate end from inlet mouth, and it is provided with air dispelling hole.
According to a further advantageous embodiment of the invention, the described gas distributor in the wherein said photoreactor be shaped as flats, be the hollow setting in it, be distributed with a plurality of apertures in top and the bottom sides of described gas distributor.
According to another preferred embodiment of the present invention, the main body of wherein said photoreactor can be at the scope internal rotation of 0-180 degree along rotating mechanical axis.
According to a further preferred embodiment of the invention, the panel of two sensitive surfaces of wherein said photoreactor is made by transparent material, and all the other four border structures are made by hollow material or monolayer material.
According to another preferred embodiment of the present invention, wherein make on the face of contact feed liquid on the hollow material of described bottom frame or the monolayer material and a plurality of holes are set as the input and output material hole.
According to a further advantageous embodiment of the invention, wherein the top frame of described photoreactor main body is provided with a plurality of Link Ports, is used for connecting sensor device.
According to a further advantageous embodiment of the invention, described photoreactor can have the above-mentioned photoreactor main body that a plurality of parallel series connection are used.
The present invention also provides a kind of method of cultivating photosynthetic microorganism, wherein adopt aforementioned photoreactor of the present invention, nutrient solution enters the photoreactor main body by opening for feed, rise from the photoreactor bottom part body in the liquid elevated areas, the influent zone that descends when being higher than long dividing plate, and flow out by discharge port, the gas that the external feed stream pipeline provides enters the photoresponse district by inlet mouth through gas distributor, nutrient solution nutrient solution through the liquid elevated areas rises and release, enter the plenum zone of described photoreactor main body, discharge and collect by the air outlet then.The gas that photosynthetic microorganism produces can be carried by the gas that the external feed stream pipeline provides or replace and discharge from nutrient solution.The liquid product that photosynthetic microorganism produces carries out reactor by nutrient solution, collects through separating purifying plant.
Above-mentioned photoreactor according to the present invention has the following advantages:
At first, the light application ratio surface-area is big, illumination utilization ratio height;
Secondly, photosynthetic microorganism and/or the nutrient solution of cultivation mix, and oxygen is resolved fast;
Again, the device air permeability is good and the nutrient solution mixed cycle is good;
At last, device cleans simple, does not cause the obstruction of device.
Description of drawings
By reading the detailed description of doing with reference to the following drawings that non-limiting example is done, it is more obvious that other features, objects and advantages of the present invention will become, wherein:
Fig. 1 is the one-piece construction synoptic diagram of described photoreactor according to a preferred embodiment of the present invention.
Fig. 2 is the vertical view of the main body of described photoreactor according to a preferred embodiment of the present invention.
Fig. 3 is the vertical view of the gas distributor in according to a preferred embodiment of the present invention the described photoreactor main body.
Fig. 4 is the side-view of the angle regulator in according to a preferred embodiment of the present invention the described photoreactor.
Respective drawings mark corresponding components title is as follows in the accompanying drawing:
100. photoreactor main body 101. inlet mouths
102. air outlet 103. opening for feeds
104. discharge port 105. mechanical axis
106. breviseptum 107. long dividing plates
108. gas distributor 109. sensitive surface panels
1101. top frame 1102. bottom frames
1103. left border 1104. Right Borders
111. sensor device Link Port 112. photoresponse districts
113. plenum zone 114. liquid elevated areas
Same or analogous Reference numeral represents same or analogous parts in the accompanying drawing.
Embodiment
For a better understanding and interpretation of the present invention, the present invention is described in further detail with reference to Fig. 1-4 below.
Fig. 1 is the one-piece construction synoptic diagram of described photoreactor according to a preferred embodiment of the present invention.As shown in Figure 1, the one-piece construction of described photoreactor is made up of three parts: photoreactor main body 100, rotating mechanical axis 105 and base for supporting 200.Wherein, photoreactor main body 100 is made up of inlet mouth 101, air outlet 102, opening for feed 103, discharge port 104, breviseptum 106, long dividing plate 107, gas distributor 108, two sensitive surface panels 109, top frame 1101, bottom frame 1102, left border 1103, Right Border 1104 and sensor device Link Ports 111, and particular location as shown in the figure.
Wherein, photoreactor main body 100 is connected by rotating mechanical axis 105 with base for supporting 200, described photoreactor can also comprise angle regulator 400 (figure 4 illustrates its structure), this angle regulator 400 is connected with base for supporting 200 with photoreactor main body 100, and photoreactor main body 100 can be rotated around described mechanical axis 105 by described angle regulator 400.The centre of photoreactor main body 100 is by two sensitive surface panels 109, and it utilizes top frame 1101, bottom frame 1102, left border 1103 and Right Border 1104 fixing all around.Two sensitive surface panels 109 form flats cubes enclosed construction with top frame 1101, bottom frame 1102, left border 1103, Right Border 1104.Usually sensitive surface panel 109 utilizes transparent material to make, and all the other four border structures utilize hollow material to make, and can certainly adopt metallic substance to make described frame; Simultaneously, can adopt monolayer material (for example metallic substance) to make described frame, as long as can reach the effect of fixing described sensitive surface panel 109, and said structure makes photoreactor main body 100 form the cubes enclosed construction of the saturating liquid of sealed air-tight, described photoreactor main body 100 is connected with external system by described inlet mouth 101, air outlet 102, opening for feed 103 and discharge port 104, be respectively applied to input and output gas or liquid, for example contain the nutrient solution that is used for culturing micro-organisms or plant of nutrient.Namely as shown in Figure 1, described photoreactor main body is flats cubes enclosed construction, and its bottom frame 1102 is connected with described base for supporting 200 by rotating mechanical axis 105.When above-mentioned top frame 1101, bottom frame 1102, left border 1103 and Right Border 1104 utilize monolayer material to make, above-mentioned each plate place of being connected and fixed all adopts synthetic glass bonding, or adopt the bonding or integrated formation of other modes, thereby make photoreactor form the cubes enclosed construction of a sealed air-tight.
Have parallel long dividing plate 107 and a plurality of breviseptum 106 that arranges in the inside of photoreactor main body 100, be divided into photoresponse district 112 and plenum zone 113 up and down according to the nutrient solution zone in the photoreactor main body 100, in addition, described photoresponse district 112 is divided into liquid elevated areas 114 by long dividing plate about 107 and liquid descends regional 115, breviseptum 106 is parallel distribution in liquid elevated areas 114, in addition, the inner face of the side of described long dividing plate 107 and photoreactor main body 100 is tightly connected, and each breviseptum 106 is tightly connected with the inner face of photoreactor main body 100.According to a preferred embodiment of the present invention, described liquid elevated areas has 8-20 bar breviseptum.
Be provided with gas distributor 108 in the bottom of described liquid elevated areas 114, preferably, it can extend to or approach long dividing plate 107 ends, and described gas distributor 108 is connected with the external feed stream pipeline by described inlet mouth 101; Described inlet mouth 101 is tubular structure, be positioned at the frame of liquid elevated areas 114 1 sides of described photoreactor main body 100, be on the illustrated frame 1104, air outlet 102 is positioned on the top frame 1101 of photoreactor main body 100, described opening for feed 103 and inlet mouth 101 homonymy settings, discharge port 104 is positioned at the frame of described liquid decline zone 115 1 sides, namely on the illustrated frame 1103.Discharge port 104 also can be positioned at the underframe (not shown) of described liquid decline zone 115 1 sides.
In photoreactor of the present invention, the nutrient solution that is used for cultivation photosynthetic microorganism or other microorganism or plant enters photoreactor main body 100 by opening for feed 103, rise from photoreactor main body 100 bottoms in liquid elevated areas 114, the influent zone 115 that descends when being higher than long dividing plate 107, and flow out by discharge port 104.The photosynthetic microorganism of cultivating or other microorganism or plant breed in nutrient solution and photoresponse, so nutrient solution rests on the photoresponse district that the interior part of reactor namely constitutes described photoreactor main body.The gas that the external feed stream pipeline provides enters the photoresponse district by gas distributor, nutrient solution nutrient solution through the liquid elevated areas rises and release, or carry from the gas of photosynthetic microorganism or other microorganism or plant generation and rise, discharge from nutrient solution, enter the headspace of described photoreactor main body, i.e. plenum zone.Photosynthetic microorganism produces useful gas and can discharge and collect by the air outlet.The liquid product that photosynthetic microorganism produces carries out reactor by nutrient solution, collects through separating purifying plant.
Wherein, described photoreactor main body 100 can be at the scope internal rotation of 0-180 degree along described rotating mechanical axis 105.Preferably, described photoreactor main body 100 can be a connection with the mechanical axis 105 that is connected of base for supporting 200, namely can realize rotating described photoreactor main body 100 according to the sunlight direction of illumination, realize that described photoreactor main body 100 is always towards the sunlight direction of illumination, keep sensitive surface vertical with the sunlight direction of illumination, accept the maximum technique effect of luminous energy.
In the actually operating and operational process of described photoreactor, by the external feed stream pipeline, as jet pump etc., gas utilizes inlet mouth 101 to enter gas distributor 108, then the pore ejection by gas distributor 108 surfaces, then mix with feed liquid, and gas distributor 108 general shape are flats, are the hollow setting in it, wherein, a side at described gas distributor 108 is furnished with a plurality of apertures, is used for as production well; Preferably, gas distributor 108 is for extending to the diffuser that approaches long dividing plate end from inlet mouth, and the left and right sides of diffuser is provided with row's air dispelling hole (not shown).Water can enter recycle system (not shown) by waterworkss such as water pumps, and then reactant mixes with water by piping system (belonging to external system, not shown), forms feed liquid, then enters photoreactor main body 100 by opening for feed 103; After gas mixed by pipeline and feed liquid, because the effect of gas, feed liquid was constantly stirred and is fully reacted in the photoresponse district 112 of photoreactor main body 100, and then gas arrives the plenum zone 113 of photoreactor main body 100, and 102 discharge from the air outlet; When entering photoreactor main body 100 by opening for feed 103, feed liquid can select directly to enter the liquid elevated areas 114 of photoreactor main body 100, feed liquid under external force (for example, can provide external force by mechanisms such as pumps) constantly rise, cross the top of long dividing plate 107, by the interval of long dividing plate 107 with the top of described photoreactor main body 100, under action of gravity, descend, enter liquid and descend zone 115 by outside the discharge port 104 outflow photoreactors that arrange on the illustrated left border 1103.Because photosynthetic microorganism is lived in the feed liquid with suspended state, described breviseptum 106 is divided into several littler spaces with liquid elevated areas 114, gas evenly distributes in each space, constantly rise, provide sufficient gas to feed liquid, simultaneously feed liquid is played the effect of a stirring, reach better circulating effect.
Preferably, when entering photoreactor main body 100, described feed liquid also can adopt other modes to enter aforesaid liquid elevated areas 114, can a plurality of holes be set as the input and output material hole at the face of the hollow material of making illustrated bottom frame 1102 or the described feed liquid of monolayer material contact, and feed liquid enters liquid elevated areas 114 (described hole is not shown) by above-mentioned pore structure.
Preferably, when utilizing hollow material to make left border 1103, pipeline can be set in hollow material, form tubulose discharge port 104.Discharge port 104 also can be positioned at the underframe (not shown) of described liquid decline zone 115 1 sides.Described feed liquid can descend by the liquid in the one side of section bar contact feed liquid and a plurality of holes is set in the scope in zone 115, and goes into above-mentioned tubulose discharge port by described orifice flow, thereby flows out outside the photoreactor main body 100.Preferably, on the bottom frame 1102 of liquid elevated areas 114 discharge port 104 is set also.In culturing process, the discharge port 104 of aforesaid liquid elevated areas is closed, and only uses in the cleaning reaction device.That is to say, when needs clean photoreactor main body 100 (for example find pollution or mass mortality appear in microorganism and when precipitating), just stop at the input of opening for feed 103, and open the discharge port 104 that arranges on the frame of liquid elevated areas 114, emit nutrient solution.This design has increased effect and the efficient of cleaning photoreactor greatly.
More preferably, also can breviseptum be set in described liquid decline zone 115, realize the stirring reaction of feed liquid.In addition, a plurality of long dividing plates 107 can be set also, middle ware is separated with breviseptum 106, gets final product and the end of described gas distributor 108 only needs and the long dividing plate of the most close illustrated left border one side is tightly connected.
In addition, the top frame 1101 of photoreactor main body 100 is provided with a plurality of Link Ports 111, is used for connecting sensor device.Described sensor device be used for to measure described photoreactor main body 100 be subjected to information such as intensity of illumination and temperature, and send conditioning signal in real time to described angle regulator 400 (shown in Figure 4), constantly adjust the angle of the main body 100 of photoreactor, thereby realize the reaction of the fullest of feed liquid under the illumination effect.In the factor owing to the breeding that influences photosynthetic microorganism and growth, influence the key factor of photosynthetic microorganism high-density culture during illumination.Photosynthetic microorganism is needed intensity of illumination difference under different upgrowth situations.Based on above-mentioned principle, be provided with rotating mechanical axis 105 and angle regulator 400, angle regulator 400 can be air pump or other angle regulators.By regulating the angle of photoreactor main body 100, the intensity of illumination that can regulate the sensitive surface panel 109 of photoreactor main body 100.
Base for supporting 200 as shown is the cube frame structure, and four base angles can utilize plastic mattress leg structure absorption ground, reaches and supports photoreactor main body 100 and the effect of fixing described photoreactor main body 100.Usually base for supporting utilizes metallic substance to make, region intermediate at described base for supporting 200 can include accessory structure simultaneously, inflator for example, water pump etc., certain described accessory structure also can be independent of above-mentioned base for supporting structure and in this base for supporting outer setting.
Fig. 2 is the vertical view of the main body 100 of described photoreactor according to a preferred embodiment of the present invention.As shown in Figure 2, has air outlet 102 on the top frame 1101 of described photoreactor main body 100 and a plurality of sensor device Link Port 111 (is illustrated as 6, arrange symmetrically), four border structures when photoreactor main body 100, be top frame 1101, bottom frame 1102, left border 1103 and Right Border 1104 when utilizing hollow material to make, described air outlet 102 and a plurality of sensor device Link Port 111 are tubular structure; When four border structures of photoreactor main body 100, when namely top frame 1101, bottom frame 1102, left border 1103 and Right Border 1104 utilized monolayer material to make, described air outlet 102 and a plurality of sensor device Link Port 111 were cavernous structure.
Shown in vertical view, long dividing plate 107 and breviseptum 106 are to have certain thickness rectangular parallelepiped diaphragm structure, breviseptum 106 is positioned at liquid elevated areas 114 and is evenly distributed with identical spacing, the lower end of long dividing plate 107 is connected with the lower seal of photoreactor main body 100, the upper end is not connected with the top frame of photoreactor main body 100, is spaced a distance; The bottom frame of the lower end of breviseptum 106 and photoreactor main body 100 at interval an end distance from, and the top frame of upper end and photoreactor main body 100 also at interval an end distance from, but above-mentioned distance is less than the distance of the top frame of long dividing plate upper end and photoreactor main body 100.Sensitive surface panel 109 utilizes four border structures, it is top frame 1101, bottom frame 1102, left border 1103 and Right Border 1104 are fixing, described sensitive surface panel 109 as shown is transparent material and makes, and four border structures, it is top frame 1101, bottom frame 1102, left border 1103 and Right Border 1104 are utilized as square hollow material, can certainly utilize metallic substance to make described frame, simultaneously, can adopt monolayer material (for example metallic substance) to make described frame, as long as can reach the effect of fixing described sensitive surface panel 109, and said structure makes photoreactor main body 100 form the cubes enclosed construction of a sealed air-tight.
Fig. 3 is the vertical view of the gas distributor in according to a preferred embodiment of the present invention the described photoreactor main body.As shown in Figure 3, described gas distributor 108 be shaped as flats, be the hollow setting in it, wherein, (being illustrated as top-side) is furnished with a plurality of apertures on the top and bottom sides of described gas distributor 108, only shows the top-side of the described gas distributor of overlooking the visual angle among the figure.The end of described gas distributor 108 is connected with long dividing plate 107 and inlet mouth 101 respectively, and preferably, gas distributor 108 can not be connected with long dividing plate 107 yet, but has certain intervals.In the making processes of reality, described gas distributor 108 adopts metallic substance usually, and for example aluminium alloy or stainless steel are made.
Preferably, described gas distributor 108 is for extending to the diffuser that approaches long dividing plate 107 1 ends, and the left and right sides of diffuser is provided with row's air dispelling hole (not shown).It is circular that the shape of the shape of described venting hole and the aperture of above-mentioned distribution is generally, and certainly, can be set to other shapes.And vapor pipe also can be set to many, and in like manner, gas distributor 108 also can adopt a plurality of, can carry out various configurations according to the demand of required air-flow in the culturing process.
Described gas distributor 108 is connected with the external feed stream pipeline by inlet mouth 101, certainly preferably, the quantity of described inlet mouth 101, external feed stream pipeline and gas distributor 108 or diffuser can be carried out multiple choices and configuration, as long as satisfy the air-flow size of gas required in culturing process.
Fig. 4 is the side-view of the angle regulator 400 in according to a preferred embodiment of the present invention the described photoreactor.As shown in Figure 4, an end 401 of described angle regulator 400 (being illustrated as a thinner end) is connected with described photoreactor main body 100, and the other end 402 (being illustrated as a thicker end) is connected with base for supporting in the described photoreactor.The two ends 401 and 402 of described angle regulator are nesting type structures, can realize the mechanical characteristics of flexible and push-and-pull, described photoreactor main body 100 can utilize the flexible and push-and-pull characteristic of angle regulator 400 to realize the effect of rotating around mechanical axis 105, thus make photoreactor main body 100 sensitive surface panel 109 be subjected to intensity of illumination adjusted.According to intensity of illumination information or temperature information that the sensor that connects by sensor device Link Port 111 on the photoreactor main body 100 is responded to, described angle regulator 400 can realize that photoreactor main body 100 is around the rotation of mechanical axis 105.
Described angle regulator 400 utilizes metallic substance to make usually, certainly according to actual needs, also can adopt photoactive material to make, realize self measurement and control of temperature, inside can be embedded with Vulcan gear simultaneously, realizes the end 401 of described angle regulator 400 and the stretching of 402 outward directions.
The invention also discloses a kind of method of cultivating photosynthetic microorganism, wherein adopt aforementioned photoreactor of the present invention, nutrient solution enters the photoreactor main body by opening for feed, rise from the photoreactor bottom part body in the liquid elevated areas, the influent zone that descends when being higher than long dividing plate, and flow out by discharge port, the gas that the external feed stream pipeline provides enters the photoresponse district by inlet mouth through gas distributor, nutrient solution through the liquid elevated areas rises and release, enter the plenum zone of described photoreactor main body, discharge and collect by the air outlet then.The gas that photosynthetic microorganism produces can be carried by the gas that the external feed stream pipeline provides or replace and discharge from nutrient solution.The liquid product that photosynthetic microorganism produces carries out reactor by nutrient solution, collects through separating purifying plant.
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned one exemplary embodiment, and under the situation that does not deviate from spirit of the present invention or essential characteristic, can realize the present invention with other specific form.Therefore, no matter from which point, all should regard embodiment as exemplary, and be nonrestrictive, scope of the present invention is limited by claims rather than above-mentioned explanation, therefore is intended to be included in the present invention dropping on the implication that is equal to important document of claim and all changes in the scope.Any Reference numeral in the claim should be considered as limit related claim.In addition, obviously miscellaneous part, unit or step do not got rid of in " comprising " word, and odd number is not got rid of plural number.A plurality of parts of stating in system's claim, unit or device also can be realized by software or hardware by parts, unit or device.
Claims (10)
1. a photoreactor comprises the photoreactor main body, and rotating mechanical axis and base for supporting is characterized in that:
Described photoreactor main body is flats cubes enclosed construction, be connected with external system with material import and export by gas inlet and outlet, described photoreactor main body is by two sensitive surface panels, top frame and bottom frame, and left border and Right Border composition, described top frame, bottom frame, left border and Right Border are used for fixing described two sensitive surface panels;
The bottom frame of described photoreactor main body is connected with described base for supporting by described rotating mechanical axis;
Have parallel long dividing plate and a plurality of breviseptum that arranges in the photoreactor body interior, described long dividing plate closely is connected with described two sensitive surface panels with breviseptum; Described long dividing plate one end is connected with described bottom frame, and the other end is not connected with the top frame, and the breviseptum two ends all are not connected with the top frame with the bottom frame, and the distance of long dividing plate and top frame is less than the distance of breviseptum and top frame; Be divided into photoresponse district and plenum zone in the photoreactor main body up and down; In addition, described photoresponse district is divided into liquid elevated areas and the liquid zone that descends about by long dividing plate, and breviseptum is positioned at the liquid elevated areas and with the parallel distribution of identical spacing;
In addition, be provided with gas distributor in the bottom of described liquid elevated areas, described gas distributor is connected with the external feed stream pipeline by inlet mouth;
Described inlet mouth is positioned on the frame of described photoreactor liquid elevated areas one side, and the air outlet is positioned on the top frame of photoreactor main body;
Opening for feed and inlet mouth homonymy arrange, and discharge port is positioned at liquid and descends on the frame or underframe of a regional side.
2. photoreactor as claimed in claim 1 wherein also comprises angle regulator, and this angle regulator is connected respectively with base for supporting with the photoreactor main body, and the photoreactor main body can be rotated around described mechanical axis by described angle regulator.
3. photoreactor as claimed in claim 2, wherein said photoreactor main body can be at the scope internal rotation of 0-180 degree along rotating mechanical axis.
4. photoreactor as claimed in claim 1, wherein said gas distributor is for extending to the diffuser that approaches long dividing plate end from inlet mouth, and it is provided with air dispelling hole.
5. photoreactor as claimed in claim 4, wherein said gas distributor be shaped as flats, be the hollow setting in it, wherein, be furnished with a plurality of apertures in top and the bottom sides of described gas distributor.
6. photoreactor as claimed in claim 1, wherein the panel of two of photoreactor sensitive surfaces is made by transparent material.
7. photoreactor as claimed in claim 1, wherein four border structures of photoreactor are made by hollow material or monolayer material.
8. photoreactor as claimed in claim 7 wherein also arranges discharge port on the bottom of liquid elevated areas frame or underframe.
9. photoreactor as claimed in claim 1 is characterized in that the main body of described photoreactor can be used in a plurality of parallel series connection.
10. method of cultivating photosynthetic microorganism, wherein adopt the photoreactor as each definition among the claim 1-9, photosynthetic microorganism is cultivated in the photoresponse district and is reacted, nutrient solution enters the photoreactor main body by opening for feed, rise from the photoreactor bottom part body in the liquid elevated areas, the influent zone that descends when being higher than long dividing plate, and flow out by discharge port, the gas that the external feed stream pipeline provides enters the photoresponse district by inlet mouth through gas distributor, rise and discharge into the plenum zone of described photoreactor main body through the nutrient solution nutrient solution of liquid elevated areas, discharge and collect by the air outlet then, in addition, the liquid product that photosynthetic microorganism produces carries out reactor by nutrient solution, collects through separating purifying plant.
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DE4134813C2 (en) * | 1991-10-22 | 1994-07-21 | Inst Getreideverarbeitung | Facility for the cultivation of phototrophic microorganisms |
CN101558146A (en) * | 2006-10-20 | 2009-10-14 | 代表亚利桑那大学的亚利桑那校董会 | System and method for growing photosynthetic cells |
WO2010068288A2 (en) * | 2008-12-11 | 2010-06-17 | Joule Biotechnologies, Inc. | Solar biofactory, photobioreactors, passive thermal regulation systems and methods for producing products |
CN102080041A (en) * | 2010-12-07 | 2011-06-01 | 江南大学 | Continuous flow column type photobiological reactor and method for continuously culturing petroliferous microalgae in large scale |
CN202265566U (en) * | 2011-09-07 | 2012-06-06 | 浙江齐成碳能科技有限公司 | Photo reactor for cultivating photosynthetic microoganisms |
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Patent Citations (5)
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DE4134813C2 (en) * | 1991-10-22 | 1994-07-21 | Inst Getreideverarbeitung | Facility for the cultivation of phototrophic microorganisms |
CN101558146A (en) * | 2006-10-20 | 2009-10-14 | 代表亚利桑那大学的亚利桑那校董会 | System and method for growing photosynthetic cells |
WO2010068288A2 (en) * | 2008-12-11 | 2010-06-17 | Joule Biotechnologies, Inc. | Solar biofactory, photobioreactors, passive thermal regulation systems and methods for producing products |
CN102080041A (en) * | 2010-12-07 | 2011-06-01 | 江南大学 | Continuous flow column type photobiological reactor and method for continuously culturing petroliferous microalgae in large scale |
CN202265566U (en) * | 2011-09-07 | 2012-06-06 | 浙江齐成碳能科技有限公司 | Photo reactor for cultivating photosynthetic microoganisms |
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