CN102424799A - Aspergillus versicolor for fermenting Jatropha curcas cake meal to prepare detoxified feed - Google Patents
Aspergillus versicolor for fermenting Jatropha curcas cake meal to prepare detoxified feed Download PDFInfo
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- CN102424799A CN102424799A CN2011104429900A CN201110442990A CN102424799A CN 102424799 A CN102424799 A CN 102424799A CN 2011104429900 A CN2011104429900 A CN 2011104429900A CN 201110442990 A CN201110442990 A CN 201110442990A CN 102424799 A CN102424799 A CN 102424799A
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- 241000203233 Aspergillus versicolor Species 0.000 title claims abstract description 23
- 241001048891 Jatropha curcas Species 0.000 title abstract description 12
- 235000012054 meals Nutrition 0.000 title abstract description 8
- 231100000331 toxic Toxicity 0.000 claims abstract description 18
- 230000002588 toxic effect Effects 0.000 claims abstract description 18
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 11
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 11
- 238000004321 preservation Methods 0.000 claims abstract description 8
- 241001465754 Metazoa Species 0.000 claims abstract description 7
- 241000228212 Aspergillus Species 0.000 claims abstract description 6
- 240000001866 Vernicia fordii Species 0.000 claims description 70
- 238000001784 detoxification Methods 0.000 claims description 15
- 230000035772 mutation Effects 0.000 claims description 5
- 231100000419 toxicity Toxicity 0.000 abstract description 9
- 230000001988 toxicity Effects 0.000 abstract description 9
- 239000001963 growth medium Substances 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 24
- 230000001580 bacterial effect Effects 0.000 description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 238000000855 fermentation Methods 0.000 description 6
- 230000004151 fermentation Effects 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 5
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- 238000001035 drying Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
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- 241000894006 Bacteria Species 0.000 description 3
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000002551 biofuel Substances 0.000 description 3
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- 229920001817 Agar Polymers 0.000 description 1
- 241000228232 Aspergillus tubingensis Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
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- 235000004977 Brassica sinapistrum Nutrition 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000221017 Euphorbiaceae Species 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- 241000221089 Jatropha Species 0.000 description 1
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 description 1
- 229910019093 NaOCl Inorganic materials 0.000 description 1
- 240000007817 Olea europaea Species 0.000 description 1
- 244000055346 Paulownia Species 0.000 description 1
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- 239000004467 fishmeal Substances 0.000 description 1
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- 239000010871 livestock manure Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
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- 229930182817 methionine Natural products 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- PHEDXBVPIONUQT-RGYGYFBISA-N phorbol 13-acetate 12-myristate Chemical compound C([C@]1(O)C(=O)C(C)=C[C@H]1[C@@]1(O)[C@H](C)[C@H]2OC(=O)CCCCCCCCCCCCC)C(CO)=C[C@H]1[C@H]1[C@]2(OC(C)=O)C1(C)C PHEDXBVPIONUQT-RGYGYFBISA-N 0.000 description 1
- 239000002644 phorbol ester Substances 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000004456 rapeseed meal Substances 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000007669 thermal treatment Methods 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
Images
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- Fertilizers (AREA)
- Fodder In General (AREA)
Abstract
A variegated aspergillus for fermenting Jatropha curcas cake meal to produce detoxified feed relates to the field of feed. The Aspergillus is named as Aspergillus versicolor A003, and the strain is preserved in a preservation unit specified by the patent office of the national intellectual property office, wherein the preservation date is 7/11 days 2011, and the name of the preservation unit is as follows: china center for type culture Collection, the preservation number is CCTCC NO: m2011249. When the invention is inoculated on a culture medium prepared by taking the Jatropha curcas cake meal as a main component, the invention can rapidly ferment and remove toxic components in the Jatropha curcas cake meal, and the Jatropha curcas cake meal after toxicity removal can be used as protein feed for feeding animals.
Description
Technical field
The invention belongs to field of fodder, relate to a kind of aspergillus versicolor of the little seeds of a tung oil tree grouts toxic composition of degrading.
Background technology
In short supply day by day along with petroleum resources greatly developed biomass energy and is the inevitable strategic choice that China improves energy security, ensures power supply.The little seeds of a tung oil tree (
Jatropha curcasL. have another name called the cream paulownia, Cortex jatrophae etc.) belong to Euphorbiaceae leprosy Pterostyrax, be considered to develop the suitable seeds of biofuel, but its industrial development also is in commitment, there are problems such as cost is higher, by product can't make full use of.
Along with rapid development of economy, China has become maximum in the world aquaculture country.Protein raw material is the basis of feed industrial development, and the in short supply and price of protein raw material is surging to have become and will become for a long time China's one of bottleneck with water industry of developing animal husbandry.
After little seeds of a tung oil tree grouts are taken off grease; Protein contnt reaches 68% sometimes up to more than 50%, and all is true albumen more than 90%; Except that Methionin, the content of other primary amino acid all is higher than the diet solids content standard that the World Food Programme (FAO) is children's formulation in 5 years old.The nutritive value of little seeds of a tung oil tree grouts is higher than the protein content of 35-50% such as dregs of beans commonly used in the feed, rapeseed meal.The coarse-fibred content of little seeds of a tung oil tree cake is lower than 18% of roughage index about 3%.Jatropha curcas oil dregs of rice external digestion rate reaches 86% at 78.6-80.6% after the thermal treatment.It is thus clear that little seeds of a tung oil tree grouts are a kind of very potential protein feed resources.But, containing more toxicant in the little seeds of a tung oil tree cake, can not directly use as feed.The little seeds of a tung oil tree cake of interpolation 16% can produce obvious toxic and side effects to mouse in feed.Even so-called nontoxic little seeds of a tung oil tree kind also has certain toxicity, just toxicity is low slightly.At present, still do not have and well remove the toxic method of little seeds of a tung oil tree grouts.If with behind the little seeds of a tung oil tree cake detoxifying as protein fodder, not only can alleviate the nervous situation of China feed protein resource, can also reduce little seeds of a tung oil tree biofuel cost significantly, help the industrialization of little seeds of a tung oil tree biofuel.The technology of the little seeds of a tung oil tree cake of exploitation ability effective elimination toxic component has important economic value.
The little seeds of a tung oil tree cake detoxifying method of having reported has: chemical process like the alkaline purification method, promptly adds NaOH, Ca (OH) in grouts
2Or NaOCl; Physical method is like heat treating process, radiation exposure and organic solvent elution method.But heat treating process can not remove toxicity, even also can increase toxicity.Alkaline purification method effect is limited, and can produce the intensive smell after the alkaline purification, destroys nutritive ingredient, the animal food refusal, and cause environmental pollution.The solvent-extraction process cost is expensive, also can residual intensive smell, influence palatability and the contaminate environment of animal.The radiation exposure poor.
Be developed to aspect the feed at little seeds of a tung oil tree cake, people such as Yuan Xiao have applied for " a kind of fish meal and preparation method (200710053524.7) with Jatropha curcas L dregs ", disclosure of the Invention the method for a kind of little seeds of a tung oil tree cake detoxification, with 7 times of little seeds of a tung oil tree grouts of methanol wash.People such as Liu Qiang have applied for " method (201110003750.0) of a kind of little seeds of a tung oil tree and cake detoxifying thereof ", and its poison-removing method is first with normal hexane lixiviate grease, and the extraction using alcohol toxic component is used in the back then, and the alcohol extracting dregs of rice are fried precipitation with steam, obtain the detoxification Jatropha curcas L dregs.People such as king main forces have also applied for " a kind of method of abstracting and removing phorbol ester from barbadosnut seed oil meal (200810201109.6) ", also are to remove Buddhist ripple ester with methanol extraction.People such as Zhang Hui have applied for " method (201010520744.8) of detoxification of a kind of barbadosnut seed dregs of rice and protein concentrate preparation ", remove the toxic component in the little seeds of a tung oil tree cake with ethanol elution.People such as Liu Qiang have applied for that " poison-removing method (201110020815.2) of leprosy seeds benevolence after a kind of de-oiling " also is that method with extraction using alcohol removes the toxic component in the cake.People such as Diao Qiyu have applied for " application (200910250517.5) of jatropha curcas detoxification meal in cattle fattening concentrated feed ", do not relate to concrete poison-removing method.Above-mentioned these patents all are to adopt methyl alcohol or ethanol to make the method for solvent lixiviate, and wash-out removes the toxic substance in the little seeds of a tung oil tree cake.There is the problem that production cost is high, the toxicity of toxalbumin does not have removal in this method.
Mikrobe can produce the abundant enzyme of kind, can degrade to complex compound, successfully has been used to remove the toxicity of multiple grouts, and fermentation method is one of various meal expeller and mealsolvent detoxifications method commonly used, makes feed like rapeseed cake, cotton seed cake detoxification.Owing to contain certain antimicrobial substance in the little seeds of a tung oil tree cake, add that its protein contnt is high, a lot of mikrobes can not grow the toxic component in the little seeds of a tung oil tree cake of can not degrading fast on little seeds of a tung oil tree cake.Wang Xinghong has applied for patent " strain is used to remove the toxic streptomyces fimicarius of little seeds of a tung oil tree grouts " (201110254056.6); Though this strain bacterial strain has detoxification efficiency preferably; But can not in grouts, add other organic matter; Like wheat bran etc., otherwise can increase the toxicity of little seeds of a tung oil tree cake on the contrary, reason it be unclear that.On the other hand, its detoxification efficiency is stable not enough.
About aspergillus versicolor A003 bacterial strain; Wang Xinghong has applied for " strain be used to ferment little seeds of a tung oil tree grouts make the aspergillus versicolor of bio-bacterial manure " (201110254026.5); This bacterial strain little seeds of a tung oil tree grouts that can ferment, the little seeds of a tung oil tree cake after the fermentation has good fertilizer effect.Through experiment discovery repeatedly, use the little seeds of a tung oil tree grouts of this bacterial strain detoxification also can be used as feed, so this patent of application.Do not see the report that aspergillus versicolor is used to produce little seeds of a tung oil tree cake detoxified feed as yet.
Summary of the invention
The purpose of this invention is to provide a strain have the little seeds of a tung oil tree cake of quick fermentation and can effective elimination little seeds of a tung oil tree grouts toxic composition the former mutation A003 of aspergillus versicolor bacterial strain (
Aspergillus versicolorA003).Being inoculated in this microorganism strains with little seeds of a tung oil tree cake is on the substratum of staple preparation, after cultivating, can removes the toxic component in the little seeds of a tung oil tree grouts.Through multiple living animal experiment, the toxic component of fermentation back grouts has been removed totally, can be used as animal-feed.
The former mutation of aspergillus tubigensis called after aspergillus versicolor of the present invention
Aspergillus versicolorA003, deposit number are CCTCC NO:M 2011249.
Aspergillus versicolor fungus strain of the present invention from the little seeds of a tung oil tree root portion of Yunnan Province of China Chuxiong from obtaining.Be deposited in the preservation of specified depositary institution of State Intellectual Property Office (depositary institution's title: Chinese typical culture collection center, preservation date are on July 11st, 2011, and deposit number is CCTCC NO:M 2011249 at present.The depositary institution address: Wuhan Luo Jiashan, in the Wuhan University, postcode 430072).
Aspergillus versicolor of the present invention well-grown all on most of substratum.Because little seeds of a tung oil tree cake contains antimicrobial substance, a lot of mikrobes can not well grow on the substratum by little seeds of a tung oil tree cake preparation.Though have some mikrobes on little seeds of a tung oil tree cake, to grow fast, can not remove the toxic component in the little seeds of a tung oil tree cake.When the former mutation A003 of aspergillus versicolor bacterial strain then can add or not add other little seeds of a tung oil tree cake that can loosen, increases ventilation or adjust the material of nutritive ingredient proportioning in little seeds of a tung oil tree cake; Toxicity in can the little seeds of a tung oil tree cake of effective elimination, the little seeds of a tung oil tree cake after the detoxification can be used as feed.
The little seeds of a tung oil tree cake of this strain fermentation is made the technical process of detoxified feed:
Other auxiliary material of little seeds of a tung oil tree grouts → allocate into → add entry → sterilization → inoculation → cultivation → drying → finished product
Little seeds of a tung oil tree cake detoxified feed making method of the present invention is made up of following steps:
One, little seeds of a tung oil tree cake cooperates starchy material or other microbial liquid culture medium culturing aspergillus versicolor A003 commonly used as seed.
Two, little seeds of a tung oil tree cake and water are mixed with solid medium or little seeds of a tung oil tree cake and the water ratio obtaining liq substratum in 1:7～15 in the ratio of 1:1～1.2.When the obtaining liq substratum, can in little seeds of a tung oil tree cake, allocate the glucide of 5%-50% into, strengthen the detoxification ability of bacterial strain.
Three, cultured bacterial classification is inserted on the little seeds of a tung oil tree cake solid medium after the sterilization or in the liquid nutrient medium mixing in the ratio of 1%-15%.
Three, under 10-30 ℃ of condition, cultivated 2-4 days.
Four, the results culture promptly can be used as animal-feed after drying.
Little seeds of a tung oil tree cake of the present invention comprises the squeeze cake that is obtained by milling process, also comprises the Jatropha curcas L dregs that obtains with the solvent extraction method.
The technical problem that the present invention will solve is to utilize the ability of the little seeds of a tung oil tree cake of aspergillus versicolor A003 strains for degrading toxic composition to remove the toxic component in the little seeds of a tung oil tree cake, solves the problem of the little seeds of a tung oil tree cake of restriction as feed.
This technology is made up of following characteristic: solid or liquid nutrient medium that preparation is a staple with little seeds of a tung oil tree cake; With medium sterilization, inoculation aspergillus versicolor A003 bacterial strain, fermentation culture; With promptly can be used as animal-feed after the cultured culture drying.
Beneficial effect is: aspergillus versicolor can be grown on little seeds of a tung oil tree cake fast, helps scale operation; Can specificity degrade toxic component in the little seeds of a tung oil tree cake, the little seeds of a tung oil tree cake after the detoxification can be as the protein fodder of animal.
Description of drawings
Fig. 1. the colonial morphology of strains A 003 on the Cha Shi substratum
The spore head and the conidium of Fig. 2 strains A 003
Fig. 3. aspergillus versicolor A003 and the phylogenetic tree of part correlation bacterium in the Genbank DB.
Strain characteristics: the bacterium colony limitation of on Cha Shi agar, growing, 25 ℃ of 7 days diameter 16mm, 10-12 days is 24-30mm, quality is the velvet shape.Bacterium colony is uneven, and is thick slightly, and the middle body protuberance is perk in various degree.Color just is a white, after be faint yellow, be secretly olive colour after again.Radial rill is arranged, and transudate does not have.The back side is khaki.Conidial head is radiation shape, diameter 25-125 μ m, and the conidium stalk is smooth, colourless.The product spore structure is double-deck, metulae 5-8 μ m * 2-3 μ m, bottle stalk 6-8 μ m * 1.5-2.5 μ m; Top capsule semisphere, diameter 9-20 μ m, about 3/4 surface can be educated.Conidium is spherical, diameter 2.0-3.0 μ m, tool spinule.
Culture condition: 28 ℃ of Cha Shi substratum.
Slant culture condition: 28 ℃ of Cha Shi substratum.
Seed culture condition: 28 ℃ of PDA liquid nutrient mediums.
Specific embodiment
1, in little seeds of a tung oil tree cake: water after 60 minutes, cools off by the ordinary method sterilization in the ratio preparing culture medium of 1:1, the former mutation A003 of inoculation aspergillus versicolor (
Aspergillus versicolorA003) bacterial strain was cultivated after 2 days, and aspergillus versicolor covers with substratum, and the little seeds of a tung oil tree cake that growth is good can obtain the little seeds of a tung oil tree cake detoxified feed of detoxification after drying.
2, in little seeds of a tung oil tree cake: the ratio obtaining liq substratum of water 1:10; Little seeds of a tung oil tree cake liquid nutrient medium 100ml packs in the 500ml triangular flask; 1 normal atmosphere sterilization is after 30 minutes, and aspergillus versicolor A003 is inoculated in cooling; 28 ℃ of 200rpm cultivate a week, and dry, dehydration promptly gets the little seeds of a tung oil tree cake of detoxification.
Claims (2)
1. a strain aspergillus versicolor, it is characterized in that the former mutation A003 of called after aspergillus versicolor (
Aspergillus versicolorA003), specified depositary institution of State Intellectual Property Office preservation now, preservation date is on July 11st, 2011, and depositary institution's name is called Chinese typical culture collection center, and deposit number is CCTCC NO:M 2011249.
2. aspergillus versicolor as claimed in claim 1; With cultivating on its substratum that to be inoculated in little seeds of a tung oil tree grouts be staple; Can on substratum, grow fast; Can remove the toxic component in the little seeds of a tung oil tree grouts, the little seeds of a tung oil tree cake after the detoxification can be used as protein fodder, is used for feeding animals.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104082525A (en) * | 2014-06-19 | 2014-10-08 | 四川大学 | Application of Klebsiella variicola in fermentation detoxification of Jatropha curcas seed cake |
| WO2017095212A1 (en) * | 2015-11-30 | 2017-06-08 | Centro De Investigación Y Asistencia En Tecnología Y Diseño Del Estado De Jalisco A.C. | Biotechnological method for the detoxification and associated production of biofuels/biolubricants from oleaginous pastes |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101113411A (en) * | 2007-06-27 | 2008-01-30 | 东华大学 | A method for inducing and preparing EGCG esterase and using the enzyme to produce EGC and gallic acid |
| WO2008092297A1 (en) * | 2007-01-24 | 2008-08-07 | Chengdu Institute Of Biology, The Chinese Academy Of Sciences | A new process for preparing natural abscisic acid |
| CN102329739A (en) * | 2011-08-31 | 2012-01-25 | 云南大学 | Aspergillus versicolor for fermenting Jatropha cake to manufacture biological bacterial manure |
-
2011
- 2011-12-27 CN CN2011104429900A patent/CN102424799A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008092297A1 (en) * | 2007-01-24 | 2008-08-07 | Chengdu Institute Of Biology, The Chinese Academy Of Sciences | A new process for preparing natural abscisic acid |
| CN101113411A (en) * | 2007-06-27 | 2008-01-30 | 东华大学 | A method for inducing and preparing EGCG esterase and using the enzyme to produce EGC and gallic acid |
| CN102329739A (en) * | 2011-08-31 | 2012-01-25 | 云南大学 | Aspergillus versicolor for fermenting Jatropha cake to manufacture biological bacterial manure |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104082525A (en) * | 2014-06-19 | 2014-10-08 | 四川大学 | Application of Klebsiella variicola in fermentation detoxification of Jatropha curcas seed cake |
| WO2017095212A1 (en) * | 2015-11-30 | 2017-06-08 | Centro De Investigación Y Asistencia En Tecnología Y Diseño Del Estado De Jalisco A.C. | Biotechnological method for the detoxification and associated production of biofuels/biolubricants from oleaginous pastes |
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Application publication date: 20120425 |