CN102424635A - Preparation method of special composite continuous cropping resistant agent for strawberries - Google Patents
Preparation method of special composite continuous cropping resistant agent for strawberries Download PDFInfo
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- CN102424635A CN102424635A CN2011102767103A CN201110276710A CN102424635A CN 102424635 A CN102424635 A CN 102424635A CN 2011102767103 A CN2011102767103 A CN 2011102767103A CN 201110276710 A CN201110276710 A CN 201110276710A CN 102424635 A CN102424635 A CN 102424635A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 54
- 239000002131 composite material Substances 0.000 title claims abstract description 31
- 238000009335 monocropping Methods 0.000 title abstract description 21
- 235000021012 strawberries Nutrition 0.000 title abstract description 6
- 240000009088 Fragaria x ananassa Species 0.000 title 1
- 239000003337 fertilizer Substances 0.000 claims abstract description 58
- 241000220223 Fragaria Species 0.000 claims abstract description 57
- 235000016623 Fragaria vesca Nutrition 0.000 claims abstract description 52
- 235000011363 Fragaria x ananassa Nutrition 0.000 claims abstract description 52
- 238000000034 method Methods 0.000 claims abstract description 49
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 39
- 239000011573 trace mineral Substances 0.000 claims abstract description 39
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 39
- 241000187395 Streptomyces microflavus Species 0.000 claims abstract description 25
- 239000007787 solid Substances 0.000 claims abstract description 19
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 8
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 7
- 235000015097 nutrients Nutrition 0.000 claims abstract description 7
- 239000011574 phosphorus Substances 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims description 38
- 229940029985 mineral supplement Drugs 0.000 claims description 30
- 230000001954 sterilising effect Effects 0.000 claims description 25
- 230000008569 process Effects 0.000 claims description 21
- PZDFRGGOXGETNN-UHFFFAOYSA-N phosphane;potassium Chemical compound P.[K] PZDFRGGOXGETNN-UHFFFAOYSA-N 0.000 claims description 20
- 230000003115 biocidal effect Effects 0.000 claims description 19
- 239000000843 powder Substances 0.000 claims description 18
- 238000007599 discharging Methods 0.000 claims description 15
- 239000002068 microbial inoculum Substances 0.000 claims description 15
- 238000004659 sterilization and disinfection Methods 0.000 claims description 15
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims description 12
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 11
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 claims description 11
- 229910052796 boron Inorganic materials 0.000 claims description 11
- 244000068988 Glycine max Species 0.000 claims description 10
- 235000010469 Glycine max Nutrition 0.000 claims description 10
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 10
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 10
- 239000011609 ammonium molybdate Substances 0.000 claims description 10
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical group [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 claims description 10
- 235000018660 ammonium molybdate Nutrition 0.000 claims description 10
- 229940010552 ammonium molybdate Drugs 0.000 claims description 10
- 230000001580 bacterial effect Effects 0.000 claims description 10
- 238000011081 inoculation Methods 0.000 claims description 10
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical group [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 10
- 239000002994 raw material Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 239000011701 zinc Substances 0.000 claims description 10
- 229910052725 zinc Inorganic materials 0.000 claims description 10
- 241000894006 Bacteria Species 0.000 claims description 9
- 229910021538 borax Inorganic materials 0.000 claims description 8
- 239000004328 sodium tetraborate Substances 0.000 claims description 8
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 8
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 8
- 229960001763 zinc sulfate Drugs 0.000 claims description 8
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 8
- 238000000855 fermentation Methods 0.000 claims description 7
- 230000004151 fermentation Effects 0.000 claims description 7
- 238000012360 testing method Methods 0.000 claims description 7
- 241000187747 Streptomyces Species 0.000 claims description 6
- RDXARWSSOJYNLI-UHFFFAOYSA-N [P].[K] Chemical compound [P].[K] RDXARWSSOJYNLI-UHFFFAOYSA-N 0.000 claims description 6
- 239000011733 molybdenum Substances 0.000 claims description 6
- 229910052750 molybdenum Inorganic materials 0.000 claims description 6
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 6
- 239000011592 zinc chloride Substances 0.000 claims description 6
- 235000005074 zinc chloride Nutrition 0.000 claims description 6
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 5
- 244000017020 Ipomoea batatas Species 0.000 claims description 5
- 235000002678 Ipomoea batatas Nutrition 0.000 claims description 5
- 229920002472 Starch Polymers 0.000 claims description 5
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 5
- 125000003118 aryl group Chemical group 0.000 claims description 5
- 239000004327 boric acid Substances 0.000 claims description 5
- 239000000796 flavoring agent Substances 0.000 claims description 5
- 235000019634 flavors Nutrition 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 5
- 239000002054 inoculum Substances 0.000 claims description 5
- 239000004571 lime Substances 0.000 claims description 5
- 239000011159 matrix material Substances 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- 244000005700 microbiome Species 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 229920001592 potato starch Polymers 0.000 claims description 5
- 238000005070 sampling Methods 0.000 claims description 5
- 238000011218 seed culture Methods 0.000 claims description 5
- 210000000582 semen Anatomy 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 239000000725 suspension Substances 0.000 claims description 5
- 239000003643 water by type Substances 0.000 claims description 5
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 4
- 229940072033 potash Drugs 0.000 claims description 4
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 4
- 235000015320 potassium carbonate Nutrition 0.000 claims description 4
- 241000233866 Fungi Species 0.000 claims description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 3
- 229910000160 potassium phosphate Inorganic materials 0.000 claims description 3
- 235000011009 potassium phosphates Nutrition 0.000 claims description 3
- OMUGFZNEOIWQOD-UHFFFAOYSA-N boric acid;zinc Chemical compound [Zn].OB(O)O OMUGFZNEOIWQOD-UHFFFAOYSA-N 0.000 claims 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 10
- 201000010099 disease Diseases 0.000 abstract description 9
- 239000002689 soil Substances 0.000 abstract description 9
- 235000013399 edible fruits Nutrition 0.000 abstract description 8
- 239000002253 acid Substances 0.000 abstract description 6
- 230000000813 microbial effect Effects 0.000 abstract description 6
- 229910052700 potassium Inorganic materials 0.000 abstract description 5
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 abstract description 4
- 239000011591 potassium Substances 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 4
- 150000001875 compounds Chemical class 0.000 abstract description 3
- 238000003958 fumigation Methods 0.000 abstract description 3
- -1 potassium-phosphorus compound Chemical class 0.000 abstract description 3
- 239000000654 additive Substances 0.000 abstract 2
- 230000000996 additive effect Effects 0.000 abstract 2
- 239000003242 anti bacterial agent Substances 0.000 abstract 2
- 229940088710 antibiotic agent Drugs 0.000 abstract 2
- 238000013329 compounding Methods 0.000 abstract 1
- 231100000086 high toxicity Toxicity 0.000 abstract 1
- 239000000047 product Substances 0.000 description 9
- 230000000694 effects Effects 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 235000018343 nutrient deficiency Nutrition 0.000 description 3
- 241000186361 Actinobacteria <class> Species 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 208000002720 Malnutrition Diseases 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 125000005619 boric acid group Chemical group 0.000 description 2
- GZUXJHMPEANEGY-UHFFFAOYSA-N bromomethane Chemical compound BrC GZUXJHMPEANEGY-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000003381 solubilizing effect Effects 0.000 description 2
- 241001124076 Aphididae Species 0.000 description 1
- 241001600407 Aphis <genus> Species 0.000 description 1
- 241000193738 Bacillus anthracis Species 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 241000194107 Bacillus megaterium Species 0.000 description 1
- 208000031968 Cadaver Diseases 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 241000221785 Erysiphales Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000855602 Nothotylenchus acris Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241000488583 Panonychus ulmi Species 0.000 description 1
- 241000193943 Pratylenchus Species 0.000 description 1
- 241000413494 Strawberry mottle virus Species 0.000 description 1
- 241000218632 Strawberry vein banding virus Species 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- 230000001427 coherent effect Effects 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 239000004567 concrete Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
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- 230000018109 developmental process Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229940102396 methyl bromide Drugs 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000021231 nutrient uptake Nutrition 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000037039 plant physiology Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229910001948 sodium oxide Inorganic materials 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 230000028070 sporulation Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- BYGOPQKDHGXNCD-UHFFFAOYSA-N tripotassium;iron(3+);hexacyanide Chemical compound [K+].[K+].[K+].[Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] BYGOPQKDHGXNCD-UHFFFAOYSA-N 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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- 239000011718 vitamin C Substances 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to preparation of a microbial compound preparation, and particularly relates to a preparation method of a composite continuous cropping resistant agent special for strawberries. The method comprises the steps of preparing a streptomyces microflavus antibiotic agent, preparing a trace element additive, and compounding a microbial fertilizer biological potassium-phosphorus compound fertilizer, the streptomyces microflavus antibiotic agent and the trace element additive in proportion to prepare the special compound continuous cropping resistant agent for strawberries. The invention solves the problems of high cost, high toxicity and the like caused by adopting a chemical fumigation method to solve the strawberry continuous cropping disease in the prior art, improves the micro-ecological environment of soil and provides phosphorus and potassium nutrient elements for the growth of strawberries. The special continuous cropping resistant agent produced by the invention can effectively realize yield increase in strawberry planting, and can improve the soluble solid content, sugar-acid ratio and other qualities of fruits.
Description
Technical field
The invention belongs to the preparation of mikrobe compound preparation, be meant a kind of preparation method of strawberry specific complex successive planting resistant agent especially.
Background technology
The strawberry berry is scarlet gorgeous, fragrant succulence, and sweet acid is good to eat, and is nutritious, is regarded as treasure in the fruit.The existing planting strawberry area in the whole nation is nearly 40,000 hectares at present, more than 20 ten thousand tons of output.Strawberry continuous cropping disease is the major issue that strawberry produces; Domestic and international research shows; The major cause that causes continuous cropping is due to the shortage and soil-borne disease of soil nutrient elements, and strawberry is 2 years general underproduction 10-15% of continuous cropping on the piece of same field, 3 years underproduction 20%-25% of continuous cropping; Continuous cropping underproduction more than 40% more than 4 years, severe patient even total crop failure.
In the strawberry cultivating process, particularly the continuous cropping plantation very easily causes the nutrient deficiency of soil, and replenishing coherent element rationally and effectively is important measures in the modern agriculture plantation.The strawberry disease is that main, common anthrax, gray mold, Powdery Mildew and root rot etc. are all propagated by bacterium and mould with soil-borne disease; Virus disease mainly contains various plants viruses such as strawberry mottle virus, strawberry light yellow edge virus, strawberry veinbanding virus, mainly by aphis propagation; The insect pest of strawberry is main has strawberry bud nematode, strawberry pratylenchus, red spider and aphid.
The method that present strawberry wants continuous cropping generally to adopt is exactly the chemical fumigation method.More than 15,000 yuan, only good for the year of issue only, stifling simultaneously is violent in toxicity with chemical substance to this method use cost mostly, and atmosphere and soil are all had serious destruction up to per hectare.Under the continuous condition of enlarged in strawberry industrialization market, the green non-pollution product demand is also enlarged day by day.The biological control that the strawberry continuous cropping is sick to the requirement of environmental protection and green food, all has very important significance to improving the strawberry competitiveness in the international market.
Along with the development of green food, green agriculture, bio-feritlizer begins to be used on a large scale on the modernized agricultural planting, and in bio-feritlizer, microbial fertilizer is few with its consumption, cost is low, action effect significantly forms and is first-selection.The potassium phosphorus composite fertilizer that colloid bacillus cereus with potassium decomposing effect and the bacillus megaterium with phosphate solubilization are processed is widely used a kind of microorganism live bacteria preparation in bio-feritlizer market in recent years.Microbial fertilizer is at culture fertility, and the raising chemical fertilizer utilization ratio purifies and rehabilitating soil, and reduce corps diseases and take place, the protection environment, and aspects such as raising crop products quality and food safety have all shown irreplaceable effect.
Streptomyces microflavus (
Streptomyces micrcflav) to be a strain have the antibiosis actinomycetes of antagonistic action to multiple pathogenic bacteria, the antibiotic fertilizer with this bacterial strain is produced not only can suppress the growth of multiple pathogenic bacteria, also has effects such as the crop of stimulation germinates, takes root.Actinomycetes fertilizer has been obtained good disease-resistant, effect of increasing production in use for many years.Producing antibiotic fertilizer with streptomyces microflavus at present has solid and two kinds of methods of liquid fermenting, and the solid fermentation production cycle is longer, unstable product quality; The liquid fermenting product exists with mycelial form, does not form spore, is unfavorable for preservation and storage on the one hand, and shelf life of products is short, and mycelial effect does not have spore good yet in application on the other hand.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of strawberry specific complex successive planting resistant agent, the method for utilizing liquid spawn and solid fermentation to combine, fermentative prodn streptomyces microflavus antibiotic fertilizer has guaranteed stable and a large amount of sporulation of quality product.Nutrient uptake feature according to strawberry adds trace element; And with the microbial-bacterial fertilizer with P and K decomposing function---biological phosphorus potash fertilizer is composite; Produce multi-functional control strawberry continuous cropping sick biotechnological formulation---strawberry specific complex successive planting resistant agent, this product both can solve by the microbial continuous cropping problem of soil-borne disease, can solve the continuous cropping problem that is caused by the soil nutritional deficiency again; Simultaneously owing to added the strain of phosphorus decomposing potassium solubilizing bacteria; Formed beneficial microbe colony at plant root, improved the micro-ecological environment of soil, also phosphorus potassium nutrition element is provided for the strawberry growth.
Overall technology design of the present invention is:
The preparation method of strawberry specific complex successive planting resistant agent comprises following process step:
The preparation of A, streptomyces microflavus antibiosis microbial inoculum
The preparation of A1, liquid seeds
Original strain is seeded to carries out shaking table in the liquid nutrient medium after the sterilization and cultivate and process liquid seeds, culture condition is 180 rev/mins of rotating speeds, 28 ℃-30 ℃ of culture temperature, culture cycle 24 hours; Add about 5 ml sterile waters during inoculation in test tube strains, sweep lawn, process bacteria suspension, 3-5 triangular flask of every inclined-plane inoculation with inoculating needle;
The aforesaid liquid substratum is made up of the raw material of following mass percent:
Semen Maydis powder 1.8%-2%, soybean cake powder 2%-2.5%, starch 0.8%-1.2%, surplus is a water;
Original strain select for use the bacterial strain streptomyces microflavus (
Streptomyces micrcflav), its deposit number is CGMCC4.891;
A2, solid fermentation
The A2-1 koji tray is cultivated: the koji tray substratum that the liquid seeds of preparation in the steps A 1 is inoculated into after the sterilization is cultivated; Inoculum size is that the mass ratio of the koji tray substratum after liquid seeds and the sterilization equals 5%-8%; Culture condition is 28 ℃-30 ℃ of temperature, humidity 50%-70%, culture cycle 5-6 days; Treat that the pink colour spore all appears in matrix, discharging during aromatic flavour;
The substratum that koji tray is cultivated is made up of the raw material of following mass percent:
Soybean cake powder 4%-5%, lime 1.5%-2%, sweet potato flour 12%-15%, sawdust 15%-20%, fine earth 33%-35%, surplus is a water;
A2-2 after-ripening: stir and smash the material piece during discharging, under 40 ℃ of-45 ℃ of conditions, be dried to that to contain the quality percentage composition be 10%-15%, pulverize and process streptomyces microflavus antibiosis microbial inoculum;
The preparation of B, trace mineral supplement
According to the requirement preparation trace mineral supplement of NY1428-2007 standard " trace element water-soluble fertilizer ", wherein boron, zinc, molybdenum, manganese element content sum are not less than 10% of trace mineral supplement total mass;
C, strawberry specific complex successive planting resistant agent composite
According to streptomyces microflavus antibiosis microbial inoculum: the biological potassium phosphorus of microbial-bacterial fertilizer composite fertilizer: the ratio of the mass ratio=30-34:65-69.2:0.8-1 of trace mineral supplement is mixed above-mentioned material and evenly processes strawberry specific complex successive planting resistant agent.
Process step and technical parameter concrete among the present invention are:
The sterilising conditions of liquid seed culture medium is that temperature is 121 ℃ in the described steps A 1, and the time is 25-35 minute.
The sterilising conditions of koji tray substratum is that temperature is 121 ℃ among the described steps A 2-1, and the time is 50-60 minute.
The sawdust fineness is 10 orders-20 orders in the koji tray substratum of described steps A 2-1, and the granularity of fine earth is 50 orders-70 orders, and the granularity of all the other solid materialss is 60 orders-80 orders.
Koji tray is cultivated the preceding sampling of discharging and is measured spore content with colony counting method among the described steps A 2-1, and spore content is not less than 8,000,000,000/gram.
Trace element in the trace mineral supplement of described step B can be selected multiple water-soluble back boron, zinc, molybdenum, the manganese element compound with ionic forms for use, does not break away from essence of the present invention.Wherein comparatively optimized technical scheme is, boron is selected from a kind of in borax or the boric acid, and zinc element is selected from a kind of in zinc sulfate or the zinc chloride, and molybdenum element is selected from ammonium molybdate, and manganese element is selected from manganous sulfate.
The trace mineral supplement of described step B is borax or boric acid according to mass ratio: the ratio preparation of zinc sulfate or zinc chloride: ammonium molybdate: manganous sulfate=45-55:25-35:15-25:10-20.
The biological potassium phosphorus of microbial-bacterial fertilizer composite fertilizer selects the GB20287-2006 standard of carrying out for use among the step C, above biological potassium phosphorus or the phosphorus potassium micro organism composite fertilizer of 200,000,000/gram of the effective bacterial content of mikrobe.A kind of in the biological potassium phosphorus of the microbial-bacterial fertilizer composite fertilizer biological potassium phosphate fertilizer of " Ji Wei " board of selecting for use Hebei province institute of microbiology to produce or composite fungus agent, the universal strain powder of " Yuyuan Garden " board that Hunan Yuyuan Biology Technology Co., Ltd produces, " A Musi " board biological phosphorus potash fertilizer that the Beijing Century Aims Biotechnology Co., Ltd produces comparatively preferably.
Utilize the experimental result of the prepared strawberry specific complex successive planting resistant agent of method of the present invention following:
Applicant's continuous cropping booth that 2 years strawberries have been planted in choosing in suburbs, Baoding carries out the result of use test of successive planting resistant agent.
Strawberry cultivars is Feng Xiang.Control group ck uses methyl bromide fumigation, and test group is used successive planting resistant agent by per hectare 60 (handling 1) and 80 (handling 2) kilogram respectively, applies in the planting pit equably before the field planting.In plantation with growing period management with apply fertilizer and all undertaken by ordinary method.
Gather commodity fruit and use scales/electronic balance weighing of gradation is added up ultimate production; Measure soluble sugar with Tripotassium iron hexacyanide method; With standard hydrogen sodium oxide titration measuring titratable acid; Measure ascorbic content with 2.6-dichloroindophenol method; Measure soluble solid with refractometer method.Wherein sugar, acid and ascorbic measuring method are the ordinary method of plant physiology and biochemistry measuring, and the measuring method of soluble solid meets the requirement of GB/T 12295-90 " mensuration-refractometer method of fruit, vegetables product soluble solid content ".
Test-results is seen table 1:
Table 1 strawberry specific complex successive planting resistant agent is to the influence of continuous cropping strawberry output and quality
Test-results shows with contrast ck to be compared, and processing 1 all has higher raising with the output of handling 2 pairs of strawberries and the quality of fruit, and output amplification has reached 6.71% and 6.84% respectively; Successive planting resistant agent is handled the sugar degree that has significantly improved strawberry fruit, has increased by 12.87% and 11.20%; Sugar-acid ratio has increased by 19.86% and 18.66%; Soluble solid has increased by 15.95% and 16.56%; Vitamin C content has increased by 23.81% and 23.25%.Improve fruit quality, also increased economic benefit.
The substantive distinguishing features that the present invention obtained is with significant technical progress:
Through the experiment proof; Adopt the product of the present invention's preparation both can solve by the microbial continuous cropping problem of soil-borne disease; Can solve the continuous cropping problem that causes by the soil nutritional deficiency again, owing to added the strain of phosphorus decomposing potassium solubilizing bacteria, form beneficial microbe colony simultaneously at plant root; Improved the micro-ecological environment of soil, also phosphorus potassium nutrition element is provided for the strawberry growth.The special-purpose successive planting resistant agent that in strawberry cultivating, uses the present invention to produce can reduce because the underproduction that the continuous cropping problem causes on average increases output 5%-10% effectively, and qualities such as the soluble solid of fruit, sugar degree, sugar-acid ratio also are significantly improved.
Embodiment
Below in conjunction with embodiment the present invention is done and to further describe; But conduct is not to qualification of the present invention; Protection scope of the present invention is as the criterion with the content of claims record, and any equivalence techniques means replacement of having done according to this specification sheets does not all break away from protection scope of the present invention.
Embodiment 1
The preparation method of strawberry specific complex successive planting resistant agent comprises following process step:
The preparation of A, streptomyces microflavus antibiosis microbial inoculum
The preparation of A1, liquid seeds
Original strain is seeded to carries out shaking table in the liquid nutrient medium after the sterilization and cultivate and process liquid seeds, culture condition is 180 rev/mins of rotating speeds, 28 ℃ of culture temperature, culture cycle 24 hours; Add about 5 ml sterile waters during inoculation in test tube strains, sweep lawn, process bacteria suspension, 3-5 triangular flask of every inclined-plane inoculation with inoculating needle;
The aforesaid liquid substratum is made up of the raw material of following mass percent:
Semen Maydis powder 1.8%, soybean cake powder 2%, starch 0.8%, surplus is a water;
Original strain select for use the bacterial strain streptomyces microflavus (
Streptomyces micrcflav), its deposit number is CGMCC4.891;
A2, solid fermentation
The A2-1 koji tray is cultivated: the koji tray substratum that the liquid seeds of preparation in the steps A 1 is inoculated into after the sterilization is cultivated; Inoculum size is that the mass ratio of the koji tray substratum after liquid seeds and the sterilization equals 5%; Culture condition is 28 ℃ of temperature, humidity 50%, culture cycle 5 days; Treat that the pink colour spore all appears in matrix, discharging during aromatic flavour;
The substratum that koji tray is cultivated is made up of the raw material of following mass percent:
Soybean cake powder 4%, lime 1.5%, sweet potato flour 12%, sawdust 15%, fine earth 33%, surplus is a water;
A2-2 after-ripening: stir and smash the material piece during discharging, under 40 ℃ of conditions, be dried to that to contain the quality percentage composition be 10%, pulverize and process streptomyces microflavus antibiosis microbial inoculum;
The preparation of B, trace mineral supplement
According to the requirement preparation trace mineral supplement of NY1428-2007 standard " trace element water-soluble fertilizer ", wherein boron, zinc, molybdenum, manganese element content sum are not less than 10% of trace mineral supplement total mass;
C, strawberry specific complex successive planting resistant agent composite
According to streptomyces microflavus antibiosis microbial inoculum: the biological potassium phosphorus of microbial-bacterial fertilizer composite fertilizer: the ratio of the mass ratio=30:69.2:0.8 of trace mineral supplement is mixed above-mentioned material and evenly processes strawberry specific complex successive planting resistant agent.
The sterilising conditions of liquid seed culture medium is that temperature is 121 ℃ in the described steps A 1, and the time is 25 minutes.
The sterilising conditions of koji tray substratum is that temperature is 121 ℃ among the described steps A 2-1, and the time is 50 minutes.
The sawdust fineness is 10 orders in the koji tray substratum of described steps A 2-1, and the granularity of fine earth is 50 orders, and the granularity of all the other solid materialss is 60 orders.
Koji tray is cultivated the preceding sampling of discharging and is measured spore content with colony counting method among the described steps A 2-1, and spore content is not less than 8,000,000,000/gram.
Boron in the trace mineral supplement of described step B is selected from borax, and zinc element is selected from zinc sulfate, and molybdenum element is selected from ammonium molybdate, and manganese element is selected from manganous sulfate.
The trace mineral supplement of described step B is a borax according to mass ratio: the ratio preparation of zinc sulfate: ammonium molybdate: manganous sulfate=45:30:15:10.
The biological potassium phosphorus of microbial-bacterial fertilizer composite fertilizer selects the GB20287-2006 standard of carrying out for use among the step C, above biological potassium phosphorus or the phosphorus potassium micro organism composite fertilizer of 200,000,000/gram of the effective bacterial content of mikrobe.Biological potassium phosphate fertilizer of " Ji Wei " board or composite fungus agent that the biological potassium phosphorus of microbial-bacterial fertilizer composite fertilizer selects for use Hebei province institute of microbiology to produce.
Embodiment 2
The preparation method of strawberry specific complex successive planting resistant agent comprises following process step:
The preparation of A, streptomyces microflavus antibiosis microbial inoculum
The preparation of A1, liquid seeds
Original strain is seeded to carries out shaking table in the liquid nutrient medium after the sterilization and cultivate and process liquid seeds, culture condition is 180 rev/mins of rotating speeds, 30 ℃ of culture temperature, culture cycle 24 hours; Add about 5 ml sterile waters during inoculation in test tube strains, sweep lawn, process bacteria suspension, 3-5 triangular flask of every inclined-plane inoculation with inoculating needle;
The aforesaid liquid substratum is made up of the raw material of following mass percent:
Semen Maydis powder 2%, soybean cake powder 2.5%, starch 1.2%, surplus is a water;
Original strain select for use the bacterial strain streptomyces microflavus (
Streptomyces micrcflav), its deposit number is CGMCC4.891;
A2, solid fermentation
The A2-1 koji tray is cultivated: the koji tray substratum that the liquid seeds of preparation in the steps A 1 is inoculated into after the sterilization is cultivated; Inoculum size is that the mass ratio of the koji tray substratum after liquid seeds and the sterilization equals 8%; Culture condition is 30 ℃ of temperature, humidity 70%, culture cycle 6 days; Treat that the pink colour spore all appears in matrix, discharging during aromatic flavour;
The substratum that koji tray is cultivated is made up of the raw material of following mass percent:
Soybean cake powder %, lime %, sweet potato flour 15%, sawdust 20%, fine earth 35%, surplus is a water;
A2-2 after-ripening: stir and smash the material piece during discharging, under 45 ℃ of conditions, be dried to that to contain the quality percentage composition be 15%, pulverize and process streptomyces microflavus antibiosis microbial inoculum;
The preparation of B, trace mineral supplement
According to the requirement preparation trace mineral supplement of NY1428-2007 standard " trace element water-soluble fertilizer ", wherein boron, zinc, molybdenum, manganese element content sum are not less than 10% of trace mineral supplement total mass;
C, strawberry specific complex successive planting resistant agent composite
According to streptomyces microflavus antibiosis microbial inoculum: the biological potassium phosphorus of microbial-bacterial fertilizer composite fertilizer: the ratio of the mass ratio=34:65:1 of trace mineral supplement is mixed above-mentioned material and evenly processes strawberry specific complex successive planting resistant agent.
The sterilising conditions of liquid seed culture medium is that temperature is 121 ℃ in the described steps A 1, and the time is 35 minutes.
The sterilising conditions of koji tray substratum is that temperature is 121 ℃ among the described steps A 2-1, and the time is 60 minutes.
The sawdust fineness is 20 orders in the koji tray substratum of described steps A 2-1, and the granularity of fine earth is 70 orders, and the granularity of all the other solid materialss is 80 orders.
Koji tray is cultivated the preceding sampling of discharging and is measured spore content with colony counting method among the described steps A 2-1, and spore content is not less than 8,000,000,000/gram.
Boron in the trace mineral supplement of described step B is selected from boric acid, and zinc element is selected from zinc chloride, and molybdenum element is selected from ammonium molybdate, and manganese element is selected from manganous sulfate.
The trace mineral supplement of described step B is a boric acid according to mass ratio: the ratio preparation of zinc chloride: ammonium molybdate: manganous sulfate=50:25:15:10.
The biological potassium phosphorus of microbial-bacterial fertilizer composite fertilizer selects the GB20287-2006 standard of carrying out for use among the step C, the universal strain powder of " Yuyuan Garden " board that the biological potassium phosphorus of microbial-bacterial fertilizer composite fertilizer selects for use Hunan Yuyuan Biology Technology Co., Ltd to produce.
Embodiment 3
The preparation method of strawberry specific complex successive planting resistant agent comprises following process step:
The preparation of A, streptomyces microflavus antibiosis microbial inoculum
The preparation of A1, liquid seeds
Original strain is seeded to carries out shaking table in the liquid nutrient medium after the sterilization and cultivate and process liquid seeds, culture condition is 180 rev/mins of rotating speeds, 29 ℃ of culture temperature, culture cycle 24 hours; Add about 5 ml sterile waters during inoculation in test tube strains, sweep lawn, process bacteria suspension, 3-5 triangular flask of every inclined-plane inoculation with inoculating needle;
The aforesaid liquid substratum is made up of the raw material of following mass percent:
Semen Maydis powder 2%, soybean cake powder 2.3%, starch 1%, surplus is a water;
Original strain select for use the bacterial strain streptomyces microflavus (
Streptomyces micrcflav), its deposit number is CGMCC4.891;
A2, solid fermentation
The A2-1 koji tray is cultivated: the koji tray substratum that the liquid seeds of preparation in the steps A 1 is inoculated into after the sterilization is cultivated; Inoculum size is that the mass ratio of the koji tray substratum after liquid seeds and the sterilization equals 5%-8%; Culture condition is 29 ℃ of temperature, humidity 60%, culture cycle 6 days; Treat that the pink colour spore all appears in matrix, discharging during aromatic flavour;
The substratum that koji tray is cultivated is made up of the raw material of following mass percent:
Soybean cake powder 4.5%, lime 1.8%, sweet potato flour 13%, sawdust 16%, fine earth 35%, surplus is a water;
A2-2 after-ripening: stir and smash the material piece during discharging, under 42 ℃ of conditions, be dried to that to contain the quality percentage composition be 13%, pulverize and process streptomyces microflavus antibiosis microbial inoculum;
The preparation of B, trace mineral supplement
According to the requirement preparation trace mineral supplement of NY1428-2007 standard " trace element water-soluble fertilizer ", wherein boron, zinc, molybdenum, manganese element content sum are not less than 10% of trace mineral supplement total mass;
C, strawberry specific complex successive planting resistant agent composite
According to streptomyces microflavus antibiosis microbial inoculum: the biological potassium phosphorus of microbial-bacterial fertilizer composite fertilizer: the ratio of the mass ratio=32:67:1 of trace mineral supplement is mixed above-mentioned material and evenly processes strawberry specific complex successive planting resistant agent.
The sterilising conditions of liquid seed culture medium is that temperature is 121 ℃ in the described steps A 1, and the time is 30 minutes.
The sterilising conditions of koji tray substratum is that temperature is 121 ℃ among the described steps A 2-1, and the time is 55 minutes.
The sawdust fineness is 10 orders-20 orders in the koji tray substratum of described steps A 2-1, and the granularity of fine earth is 60 orders, and the granularity of all the other solid materialss is 70 orders.
Koji tray is cultivated the preceding sampling of discharging and is measured spore content with colony counting method among the described steps A 2-1, and spore content is not less than 8,000,000,000/gram.
Boron in the trace mineral supplement of described step B is selected from borax, and zinc element is selected from zinc sulfate, and molybdenum element is selected from ammonium molybdate, and manganese element is selected from manganous sulfate.
The trace mineral supplement of described step B is a borax according to mass ratio: the ratio preparation of zinc sulfate: ammonium molybdate: manganous sulfate=50:25:10:15.
The biological potassium phosphorus of microbial-bacterial fertilizer composite fertilizer selects the GB20287-2006 standard of carrying out for use among the step C, above biological potassium phosphorus or the phosphorus potassium micro organism composite fertilizer of 200,000,000/gram of the effective bacterial content of mikrobe." A Musi " board biological phosphorus potash fertilizer that the biological potassium phosphorus of microbial-bacterial fertilizer composite fertilizer selects for use the Beijing Century Aims Biotechnology Co., Ltd to produce.
Claims (9)
1. the preparation method of strawberry specific complex successive planting resistant agent is characterized in that comprising following process step:
The preparation of A, streptomyces microflavus antibiosis microbial inoculum
The preparation of A1, liquid seeds
Original strain is seeded to carries out shaking table in the liquid nutrient medium after the sterilization and cultivate and process liquid seeds, culture condition is 180 rev/mins of rotating speeds, 28 ℃-30 ℃ of culture temperature, culture cycle 24 hours; Add about 5 ml sterile waters during inoculation in test tube strains, sweep lawn, process bacteria suspension, 3-5 triangular flask of every inclined-plane inoculation with inoculating needle;
The aforesaid liquid substratum is made up of the raw material of following mass percent:
Semen Maydis powder 1.8%-2%, soybean cake powder 2%-2.5%, starch 0.8%-1.2%, surplus is a water;
Original strain select for use the bacterial strain streptomyces microflavus (
Streptomyces micrcflav), its deposit number is CGMCC4.891;
A2, solid fermentation
The A2-1 koji tray is cultivated: the koji tray substratum that the liquid seeds of preparation in the steps A 1 is inoculated into after the sterilization is cultivated; Inoculum size is that the mass ratio of the koji tray substratum after liquid seeds and the sterilization equals 5%-8%; Culture condition is 28 ℃-30 ℃ of temperature, humidity 50%-70%, culture cycle 5-6 days; Treat that the pink colour spore all appears in matrix, discharging during aromatic flavour;
The substratum that koji tray is cultivated is made up of the raw material of following mass percent:
Soybean cake powder 4%-5%, lime 1.5%-2%, sweet potato flour 12%-15%, sawdust 15%-20%, fine earth 33%-35%, surplus is a water;
A2-2 after-ripening: stir and smash the material piece during discharging, under 40 ℃ of-45 ℃ of conditions, be dried to that to contain the quality percentage composition be 10%-15%, pulverize and process streptomyces microflavus antibiosis microbial inoculum;
The preparation of B, trace mineral supplement
According to the requirement preparation trace mineral supplement of NY1428-2007 standard " trace element water-soluble fertilizer ", wherein boron, zinc, molybdenum, manganese element content sum are not less than 10% of trace mineral supplement total mass;
C, strawberry specific complex successive planting resistant agent composite
According to streptomyces microflavus antibiosis microbial inoculum: the biological potassium phosphorus of microbial-bacterial fertilizer composite fertilizer: the ratio of the mass ratio=30-34:65-69.2:0.8-1 of trace mineral supplement is mixed above-mentioned material and evenly processes strawberry specific complex successive planting resistant agent.
2. the preparation method of strawberry specific complex successive planting resistant agent according to claim 1, the sterilising conditions that it is characterized in that liquid seed culture medium in the described steps A 1 is that temperature is 121 ℃, the time is 25-35 minute.
3. the preparation method of strawberry specific complex successive planting resistant agent according to claim 1, the sterilising conditions that it is characterized in that koji tray substratum among the described steps A 2-1 is that temperature is 121 ℃, the time is 50-60 minute.
4. the preparation method of strawberry specific complex successive planting resistant agent according to claim 1; It is characterized in that the sawdust fineness is 10 orders-20 orders in the koji tray substratum of described steps A 2-1; The granularity of fine earth is 50 orders-70 orders, and the granularity of all the other solid materialss is 60 orders-80 orders.
5. the preparation method of strawberry specific complex successive planting resistant agent according to claim 1 is characterized in that koji tray is cultivated the preceding sampling of discharging and measured spore content with colony counting method among the described steps A 2-1, and spore content is not less than 8,000,000,000/gram.
6. the preparation method of strawberry specific complex successive planting resistant agent according to claim 1; It is characterized in that boron in the trace mineral supplement of described step B is selected from a kind of in borax or the boric acid; Zinc element is selected from a kind of in zinc sulfate or the zinc chloride; Molybdenum element is selected from ammonium molybdate, and manganese element is selected from manganous sulfate.
7. the preparation method of strawberry specific complex successive planting resistant agent according to claim 6 is characterized in that the trace mineral supplement of described step B is borax or boric acid according to mass ratio: the ratio preparation of zinc sulfate or zinc chloride: ammonium molybdate: manganous sulfate=45-55:25-35:15-25:10-20.
8. the preparation method of strawberry specific complex successive planting resistant agent according to claim 1; It is characterized in that the biological potassium phosphorus of microbial-bacterial fertilizer composite fertilizer selects the GB20287-2006 standard of carrying out for use among the described step C, above biological potassium phosphorus or the phosphorus potassium micro organism composite fertilizer of 200,000,000/gram of the effective bacterial content of mikrobe.
9. according to the preparation method of claim 1 or 8 described strawberry specific complex successive planting resistant agents, it is characterized in that a kind of in the biological potassium phosphorus of microbial-bacterial fertilizer composite fertilizer selects for use Hebei province institute of microbiology to produce among the described step C biological potassium phosphate fertilizer of " Ji Wei " board or composite fungus agent, the universal strain powder of " Yuyuan Garden " board that Hunan Yuyuan Biology Technology Co., Ltd produces, " A Musi " board biological phosphorus potash fertilizer that the Beijing Century Aims Biotechnology Co., Ltd produces.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102838416A (en) * | 2012-09-19 | 2012-12-26 | 河北省微生物研究所 | Liquid biological potassium fertilizer protecting agent |
| CN103380693A (en) * | 2013-07-23 | 2013-11-06 | 安徽向荣农林生态产业有限公司 | Strawberry culture method |
| CN105084996A (en) * | 2014-05-14 | 2015-11-25 | 黄庆禄 | Preparation and field operation method of ecological planting targeted fertilization drug |
| CN109180275A (en) * | 2018-11-06 | 2019-01-11 | 天津天丰泽田生物科技有限公司 | A kind of disease in replanting prevention and treatment organic nutrition type foliar fertilizer |
| CN109315242A (en) * | 2017-07-31 | 2019-02-12 | 南丹兴农科技有限公司 | The implantation methods of leaf vegetables |
| CN110982740A (en) * | 2019-12-06 | 2020-04-10 | 山东蔚蓝生物科技有限公司 | Paenibacillus mucilaginosus and application thereof |
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1199723A (en) * | 1998-06-01 | 1998-11-25 | 四川四达生物工程产业开发股份有限公司 | Biological active composite fertilizer and its production process |
| CN1442477A (en) * | 2003-04-01 | 2003-09-17 | 青岛地恩地生物科技有限公司 | Solid fermentation process of streptomyces flavus and its production equipment |
| CN1442476A (en) * | 2003-04-01 | 2003-09-17 | 青岛地恩地生物科技有限公司 | Liquid fermentation process of streptomyces flavus and its production equipment |
| CN1456540A (en) * | 2003-03-31 | 2003-11-19 | 高银相 | High-performance composite biological nuisanceless granular fertilizer and producing method thereof |
| CN1927773A (en) * | 2006-09-22 | 2007-03-14 | 韩大山 | Anti-continuous-cropping and continuous-cropping fertilizer for agronomic crop |
| CN1986417A (en) * | 2006-12-27 | 2007-06-27 | 北京中龙创科技有限公司 | Composite microbial fertilizer for preventing and controlling diseases of continuous crop and its preparing method |
| CN101318865A (en) * | 2008-07-16 | 2008-12-10 | 张迎秋 | Fertilizer for soil sterilization for preventing and controlling continuous cropping disease |
| CN101497870A (en) * | 2009-03-02 | 2009-08-05 | 北京市京圃园生物工程有限公司 | Microbial complex bacterial agent and use thereof |
| CN101564046A (en) * | 2009-06-09 | 2009-10-28 | 南开大学 | Bacterial preparation for killing wireworm and preparation method thereof |
| CN101822273A (en) * | 2010-04-21 | 2010-09-08 | 中国农业科学院草原研究所 | Application of streptomyces microflavus in alfalfa disease control |
| CN101898915A (en) * | 2009-05-26 | 2010-12-01 | 北京嘉博文生物科技有限公司 | Microbial agent suitable for use in crop seedling stage and preparation method thereof |
| CN101990907A (en) * | 2010-08-06 | 2011-03-30 | 湖北省农业科学院植保土肥研究所 | Composite biological antimicrobial for preventing wilt and greensickness of cotton |
-
2011
- 2011-09-19 CN CN201110276710.3A patent/CN102424635B/en active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1199723A (en) * | 1998-06-01 | 1998-11-25 | 四川四达生物工程产业开发股份有限公司 | Biological active composite fertilizer and its production process |
| CN1456540A (en) * | 2003-03-31 | 2003-11-19 | 高银相 | High-performance composite biological nuisanceless granular fertilizer and producing method thereof |
| CN1442477A (en) * | 2003-04-01 | 2003-09-17 | 青岛地恩地生物科技有限公司 | Solid fermentation process of streptomyces flavus and its production equipment |
| CN1442476A (en) * | 2003-04-01 | 2003-09-17 | 青岛地恩地生物科技有限公司 | Liquid fermentation process of streptomyces flavus and its production equipment |
| CN1927773A (en) * | 2006-09-22 | 2007-03-14 | 韩大山 | Anti-continuous-cropping and continuous-cropping fertilizer for agronomic crop |
| CN1986417A (en) * | 2006-12-27 | 2007-06-27 | 北京中龙创科技有限公司 | Composite microbial fertilizer for preventing and controlling diseases of continuous crop and its preparing method |
| CN101318865A (en) * | 2008-07-16 | 2008-12-10 | 张迎秋 | Fertilizer for soil sterilization for preventing and controlling continuous cropping disease |
| CN101497870A (en) * | 2009-03-02 | 2009-08-05 | 北京市京圃园生物工程有限公司 | Microbial complex bacterial agent and use thereof |
| CN101898915A (en) * | 2009-05-26 | 2010-12-01 | 北京嘉博文生物科技有限公司 | Microbial agent suitable for use in crop seedling stage and preparation method thereof |
| CN101564046A (en) * | 2009-06-09 | 2009-10-28 | 南开大学 | Bacterial preparation for killing wireworm and preparation method thereof |
| CN101822273A (en) * | 2010-04-21 | 2010-09-08 | 中国农业科学院草原研究所 | Application of streptomyces microflavus in alfalfa disease control |
| CN101990907A (en) * | 2010-08-06 | 2011-03-30 | 湖北省农业科学院植保土肥研究所 | Composite biological antimicrobial for preventing wilt and greensickness of cotton |
Non-Patent Citations (6)
| Title |
|---|
| 孙敬祖等: "放线菌制剂对连作草莓根区微生物区系的影响及其防病促生作用", 《西北农林科技大学学报（自然科学版）》, vol. 37, no. 12, 10 December 2009 (2009-12-10), pages 153 - 158 * |
| 张丽萍等: "土壤微生物制剂防治草莓连作病害的研究", 《土壤》, vol. 39, no. 4, 15 August 2007 (2007-08-15), pages 604 - 607 * |
| 张洪昌等: "《肥料应用手册》", 31 January 2011, article "抗生菌肥料", pages: 326-328 * |
| 李保会等: "复合微生物菌肥对连作草莓产量和品质的影响", 《河北农业科学》, vol. 11, no. 1, 28 February 2007 (2007-02-28), pages 15 - 17 * |
| 李智等: "放线菌固体培养基原料筛选与确定的研究", 《黑龙江农业科学》, no. 5, 10 September 2003 (2003-09-10), pages 30 - 31 * |
| 胡江春等: "大豆连作障碍研究III 海洋放线菌MB-97促进连作大豆增产机理", 《应用生态学报》, vol. 13, no. 9, 30 September 2002 (2002-09-30), pages 1095 - 1098 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102838416A (en) * | 2012-09-19 | 2012-12-26 | 河北省微生物研究所 | Liquid biological potassium fertilizer protecting agent |
| CN103380693A (en) * | 2013-07-23 | 2013-11-06 | 安徽向荣农林生态产业有限公司 | Strawberry culture method |
| CN105084996A (en) * | 2014-05-14 | 2015-11-25 | 黄庆禄 | Preparation and field operation method of ecological planting targeted fertilization drug |
| CN109315242A (en) * | 2017-07-31 | 2019-02-12 | 南丹兴农科技有限公司 | The implantation methods of leaf vegetables |
| CN109180275A (en) * | 2018-11-06 | 2019-01-11 | 天津天丰泽田生物科技有限公司 | A kind of disease in replanting prevention and treatment organic nutrition type foliar fertilizer |
| CN110982740A (en) * | 2019-12-06 | 2020-04-10 | 山东蔚蓝生物科技有限公司 | Paenibacillus mucilaginosus and application thereof |
| CN117800784A (en) * | 2023-12-27 | 2024-04-02 | 长春奢爱农业科技发展有限公司 | Biological compound fertilizer and preparation method thereof |
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Address after: No. 2089, Wusi Middle Road, Baoding City, Hebei Province 071051 Patentee after: Hebei Institute of Microbiology Co.,Ltd. Address before: No. 2089, Wusi Middle Road, Baoding City, Hebei Province 071051 Patentee before: HEBEI Research Institute OF MICROBIOLOGY |