CN102379361A - Antibiotic-free culture method of combined application of bacteria for feed, drinking water and sterilizing and biological isolation - Google Patents
Antibiotic-free culture method of combined application of bacteria for feed, drinking water and sterilizing and biological isolation Download PDFInfo
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Abstract
The invention belongs to the field of animal husbandry, in particular relates to an antibiotic-free culture method of combined application of bacteria for feed, drinking water and sterilizing and biological isolation. The method is characterized by comprising the following four aspects of: (1) adding a strain for feed into the fermented feed; (2) adding a bacterial suspension for drinking water into routine drinking water; (3) spraying a bacterial suspension for sterilizing in the feeding environment; and (4) planting greening trees and Chinese herbal medicines with sterilizing action to replace isolation strips between adjacent colony houses. The method provided by the invention obviously improves the feeding environment, improves the culture profit, lowers the feeding cost and culture risk, and provides a strong guarantee for obtaining high-quality antibiotic-free products.
Description
Technical field
The invention belongs to the animal husbandry field, be specifically related to a kind of feed, drinking-water, sterilization with bacterium and biological nonreactive cultural method of isolating associating.
Background technology
Along with the continuous expansion of breeding scale level and the raising of world agriculture economic globalization degree.China's animal husbandry faces unprecedented development space and huge challenge for existence.At present, China's animal husbandry exists many problems and difficulty, and for example: requiring of (1) human major product and byproduct to animal husbandry is increasingly high; (2) eqpidemic disease is more and more serious to the threat of the health of animal population and breeding environment; (3) the crop products price is constantly soaring, causes animal husbandry pressure increasing; (4) the international trade barrier influences the fast development of China's animal husbandry; (5) the breed pollution problem is more and more outstanding, becomes the bottleneck of herding enterprise development, and has caused the great attention of society and government; (6) abuse of antibiotic etc not only to animal, even causes bigger hidden danger to health.
The existence of these problems has produced tremendous influence to animal husbandry, so herding enterprise all begins actively to seek and a kind ofly can improve animal welfare, reduce environmental pollution, can improve the cultural method of consumption safety again.
At present, in animal husbandry is cultured, add useful microorganism species and become a scientific and reasonable technology, but these bacterial classifications of how arranging in pairs or groups have become the project that each enterprise falls over each other to study.
The EM bacterium is that a kind of Mixed Microbes generally comprises gemma bacterium, saccharomycete, actinomyces, nitrogen-fixing bacteria, cellulose-decomposing bacterium, lactic acid bacteria etc., cultivates the effectively compound microbial flora that forms through specific process.Can be used for food and add, culture disease control, waste water control etc.
Bacillus licheniformis cellular morphology and arrangement are shaft-like, Dan Sheng; Can adjust flora (Bifidobacterium Bifidum, Bacillus acidi lactici, bacteroid, peptostreptococcus are had the growth of promotion effect) imbalance and reach therapeutic purposes, can impel body to produce antibacterial substance, kill pathogenic bacteria (pathogenic bacteria such as staphylococcus, yeast-like fungus are had antagonism).Can produce the resistant activity material, and have unique biological and take the oxygen mechanism of action by force, can suppress the growth and breeding of pathogenic bacteria.
Bacillus subtilis is a kind of of bacillus.The subtilin that produces in the bacillus subtilis thalli growth process, polymyxins, nystatin, gramicidins isoreactivity material, these active materials have the obvious suppression effect to the conditioned pathogen of pathogenic bacteria or autogenous infection.Bacillus subtilis consumes the free oxygen in the environment rapidly, causes the enteron aisle hypoxemia, promotes the growth of useful anaerobic bacteria, and produces organic acid such as lactic acid, reduces the enteron aisle pH value, suppresses other pathogenic bacteria growth indirectly.Thalline self synthesizes enzymes such as AMS, protease, lipase, cellulase, in alimentary canal with animal body in digestive enzymes together play a role; Multiple B family vitamins such as ability synthetic vitamin B1, B2, B6, nicotinic acid, the activity of interferon and macrophage in the raising animal body.
Candida tropicalis is at no O
2The time, decompose monose and produce CO
2And energy; O is arranged
2The time, monose thoroughly is decomposed into CO
2And H
2O.Candida tropicalis has very high nutritive value, particularly contains more protein, and a lot of B family vitamins, nucleic acid and mineral matter also can produce some health care active materials simultaneously.The candida tropicalis fermentation culture medium has the interferon of enhancing to tire, thereby the enhancing body immunologic function is arranged.
Lactobacillus acidophilus belongs to lactobacillus, Gram-positive bacillus, and bar terminal rounded mainly exists in the small intestine, and adjustment gut flora balance suppresses the propagation of enteron aisle undesirable microorganism.Lactobacillus acidophilus has antagonism to pathogenic microorganisms.Lactobacillus acidophilus also can produce lactic acid and acetic acid after in enteron aisle, fermenting, and can improve the utilization rate of calcium, phosphorus, iron; Promote the absorption of iron and vitamin D; Produce vitamin K and Cobastab, can also reduce absorption of cholesterol, and can reduce the injury of radiation human body.
Bafillus natto is to have beneficial bacterium acidproof, heat-resistant quality; Four hours survival rates are 100% under hydrochloric acid in gastric juice, have powerful pathogen simultaneously and suppress ability, are to environment tolerance one of the bacterial classification of small intestine that preferably can go directly in the middle of the various beneficial bacterium; It is ecological to change the animal intestinal flora after oral; The function that helps digest normalization so that defecation is smooth and easy, is kept the body physiological environmental protection.Can produce acid, regulate gut flora, the enhancing animal cell immunity is put should.And can generate multiple protein enzyme (particularly alkali protease), carbohydrase, lipase, amylase, some complicated carbohydrate of degrading plant forage kind, thereby the conversion ratio of raising feed.
Streptococcus fecalis is enterococcus faecalis again.Use the C polysaccharide antigen,, can streptococcus be divided into many crowds according to the Lan Shi serological classification.Streptococcus fecalis belongs to D crowd, and the D group streptococcus divides two types of enterococcus and non-enterococcus.The former comprises streptococcus fecalis, streptococcus faecalis and streptococcus durans, and the latter has bargen's streptococcus and streptococcus equinus.Streptococcus fecalis is an additive for farm animal feed, can prevent and treat the cub fledgling poultry because of the diarrhea that intestinal bacilli illness causes, has the effect of regulating the normal intestinal flora imbalance and promoting growth.
Bifidobacterium can be safeguarded enteron aisle normal bacteria colony balance, suppresses growth of pathogenic bacteria, prevents constipation, diarrhea and gastrointestinal disorders etc.Ability synthetic vitamin, amino acid and raising body reduce cholesterol levels in the blood to the absorption of calcium ion in enteron aisle, prevent and treat hypertension, and improve the anti-lactose property of dairy products, improve digestibility.Safeguard enteron aisle normal bacteria colony balance, suppress growth of pathogenic bacteria, have the disorderly effect of adjustment function of intestinal canal; Synthetic vitamin, amino acid and raising body are to the absorption of calcium ion in enteron aisle; The enhances human body immunity function prevents antibiotic side effect.
Summary of the invention
The purpose of this invention is to provide a kind of feed, drinking-water, sterilization with bacterium and biological nonreactive cultural method of isolating associating; Can each bacterial classification be carried out reasonably combined and then is applied in each link of breed; And take biological the isolation to make that whole breed is more scientific and reasonable; Avoid the eqpidemic disease and the problem of environmental pollution that occur in the breeding process, improve the quality of animal husbandry major product and byproduct.
A kind of feed of the present invention, drinking-water, sterilization comprise following four aspects with bacterium and biological nonreactive cultural method of isolating associating:
(1) according to adding the standard of 2～4kg feed in the feed per ton with bacterial classification; In feed, add feed and use bacterial classification; Wherein, It is 50～10,000,000,000 viable bacterias/g that feed uses the bacterial content of bacterial classification, and according to the parts by weight meter, feed is formulated by 2～3 parts of bacillus licheniformis and 1 part of bacillus subtilis with bacterial classification;
(2) the drinking water bacterial classification is processed the drinking water bacteria suspension that concentration is 50～10,000,000,000 viable bacterias/mL; The drinking water bacteria suspension compares thin up according to 1: 80～160 quality then; Maintain the standard of 1.5～300,000,000 viable bacterias/kg according to beneficial bacterium valid density in the animal body; In the daily drinking water of biology, add the drinking water bacteria suspension; Wherein, according to the parts by weight meter, the drinking water bacterial classification is formulated by 2～3 parts of lactobacillus acidophilus, 2～3 parts of bacillus licheniformis, 1 part of bacillus subtilis, 2～3 portions of candida tropicalises, 1～2 part of bafillus natto, 1 part of streptococcus fecalis and 1～2 part of Bifidobacterium;
(3) will sterilize that to use bacterial classification to process concentration be that bacteria suspension use in the sterilization of 50～10,000,000,000 viable bacterias/mL, then sterilization with bacteria suspension according to 1: 50～100 quality than thin up, spray disinfectant in feeding environment wherein, is sterilized with bacterial classification employing EM bacterium at last;
(4) plantation landscape tree and the Chinese herbal medicine with disinfective action replace the isolation strip of adjacent turn my humble abode.
Wherein, in aspect (2), hour can suitably improve the dilution ratio of drinking-water, then will suitably turn down the dilution ratio of drinking water when opposite amount of drinking water is big with bacteria suspension with bacteria suspension at institute's domesticated animal amount of drinking water.
Landscape tree in the aspect (4) is preferably one or more in Chinese wax, method paulownia or the Chinese scholar tree, and Chinese herbal medicine is preferably honeysuckle.Landscape tree of being planted and Chinese herbal medicine are isolated the transfer barrier that forms daily pathogen diffusion as biology; Guarantee that occurring in eqpidemic disease that few generation between the letting animals feed of back even do not take place with air is infectious disease popular of main route of transmission, avoids as far as possible even stop the cross-infection between the letting animals feed eqpidemic disease.
The present invention does not add any antibiotic in whole breeding process, reduce aquaculture cost, and avoided the healthy hidden danger of antibiotic to animal and human's body.
The invention has the advantages that: in breeding process; More than four aspect Combined application; Add scientific and reasonable feeding and management; Guaranteeing that letting animals feed obtains under the condition of suitable growth temperature, suppressing to be harmful to growing of bacterium in the feeding environment, reducing ill probability through drinking-water, feed and environment disinfected advantageous effect with microorganism.The air borne approach of the plantation pathogenic microorganism capable of blocking through landscape tree and Chinese herbal medicine is being avoided importing into and reducing to reduce aspect the cross-infection and culture risk of eqpidemic disease.The present invention has obviously improved feeding environment, has improved the breed income, reduced feeding cost and has cultured risk, for obtaining high-quality nonreactive product powerful guarantee is provided.
The specific embodiment
Below in conjunction with instance the present invention is further specified.
Embodiment 1:
Present embodiment is an example with the feeding of broiler, comprises following 4 aspects:
(1) according to adding the standard of 4kg feed in the feed per ton with bacterial classification; In feed, add feed and use bacterial classification, wherein, it is 7,000,000,000 viable bacterias/g that feed uses the bacterial classification content of bacterial classification; According to the parts by weight meter, feed is formulated by 3 parts of bacillus licheniformis and 1 part of bacillus subtilis with bacterial classification;
(2) the drinking water bacterial classification is mixed with the drinking water bacteria suspension that concentration is 9,000,000,000 viable bacterias/mL; The drinking water bacteria suspension compares thin up according to 1: 120 quality then; Beneficial bacterium valid density maintains the standard of 200,000,000 viable bacterias/kg in according to animal body; In biological drinking water, add the drinking water bacteria suspension after diluting; Wherein, according to the parts by weight meter, the drinking water bacterial classification is formulated by 2 parts of lactobacillus acidophilus, 2 parts of bacillus licheniformis, 1 part of bacillus subtilis, 2 portions of candida tropicalises, 2 parts of bafillus nattos, 1 part of streptococcus fecalis and 2 parts of Bifidobacteriums;
(3) will sterilize that to use bacterial classification to be mixed with concentration be that bacteria suspension use in the sterilization of 8,000,000,000 viable bacterias/mL, then sterilization with bacteria suspension according to 1: 60 quality than thin up, spray disinfectant in feeding environment wherein, is sterilized with bacterial classification employing EM bacterium at last;
(4) alternately plant the isolation strip that Chinese wax, method paulownia, Chinese scholar tree, honeysuckle replace the adjacent turn my humble abode.
Each item achievement data that present embodiment obtains is following:
(1) general index:
| Project | Breeding cycle | Feedstuff-meat ratio | Deliver average weight for sale | Survival rate | Case fatality rate | Antibiotic uses |
| Numerical value | 42d | 1.62∶1 | 2.74Kg | 95％ | 0 | Do not have |
(2) the residual detection of medicine: (detecting index is standard with the weight of animals)
| Sequence number | Project | Result (μ g/Kg) | Sequence number | Project | Result (μ g/Kg) |
| 1 | Nitroimidazole | Do not detect | 9 | Clenbuterol | 0 |
| 2 | Sulfanilamide (SN) (15) | 0 | 10 | Quinolone (13) | 0 |
| 3 | Tetracycline (4) | Do not detect | 11 | Salbutamol | 0 |
| 4 | Chloramphenicol | 0 | 12 | Ractopamine | 0 |
| 5 | Nitrofurazone | 0 | 13 | ROX | 0 |
| 6 | Furaltadone | 0 | 14 | Tylosin | 0 |
| 7 | Furantoin | 0 | 15 | Lincomycin | 0 |
| 8 | Furazolidone | 0 | 16 | Erythromycin | 0 |
Embodiment 2:
It is example that present embodiment is raised with the meat duck, comprises following 4 aspects:
(1) according to adding the standard of 4kg feed in the feed per ton with bacterial classification; In feed, add feed and use bacterial classification, wherein, it is 7,000,000,000 viable bacterias/g that feed uses the bacterial classification content of bacterial classification; According to the parts by weight meter, feed is formulated by 3 parts of bacillus licheniformis and 1 part of bacillus subtilis with bacterial classification;
(2) the drinking water bacterial classification is mixed with the drinking water bacteria suspension that concentration is 8,000,000,000 viable bacterias/mL; The drinking water bacteria suspension compares thin up according to 1: 150 quality then; Beneficial bacterium valid density maintains the standard of 2.5 hundred million viable bacterias/kg in according to animal body; In biological drinking water, add the drinking water bacteria suspension after diluting; Wherein, according to the parts by weight meter, the drinking water bacterial classification is formulated by 2 parts of lactobacillus acidophilus, 2 parts of bacillus licheniformis, 1 part of bacillus subtilis, 2 portions of candida tropicalises, 2 parts of bafillus nattos, 1 part of streptococcus fecalis and 2 parts of Bifidobacteriums;
(3) will sterilize that to use bacterial classification to be mixed with concentration be that bacteria suspension use in the sterilization of 7,000,000,000 viable bacterias/mL, then sterilization with bacteria suspension according to 1: 80 quality than thin up, spray disinfectant in feeding environment wherein, is sterilized with bacterial classification employing EM bacterium at last;
(4) alternately plant the isolation strip that Chinese wax, method paulownia, Chinese scholar tree, honeysuckle replace the adjacent turn my humble abode.
Each item achievement data that present embodiment obtains is following:
(1) general index:
| Project | Breeding cycle | Feedstuff-meat ratio | Deliver average weight for sale | Survival rate | Case fatality rate | Antibiotic uses |
| Numerical value | 42d | 1.82∶1 | 3.33Kg | 95％ | 0 | Do not have |
(2) the residual detection of medicine: (detecting index is standard with the weight of animals)
| Sequence number | Project | Result (μ g/Kg) | Sequence number | Project | Result (μ g/Kg) |
| 1 | Nitroimidazole | Do not detect | 9 | Clenbuterol | 0 |
| 2 | Sulfanilamide (SN) (15) | 0 | 10 | Quinolone (13) | 0 |
| 3 | Tetracycline (4) | Do not detect | 11 | Salbutamol | 0 |
| 4 | Chloramphenicol | 0 | 12 | Ractopamine | 0 |
| 5 | Nitrofurazone | 0 | 13 | ROX | 0 |
| 6 | Furaltadone | 0 | 14 | Tylosin | 0 |
| 7 | Furantoin | 0 | 15 | Lincomycin | 0 |
| 8 | Furazolidone | 0 | 16 | Erythromycin | 0 |
Claims (3)
1. a feed, drinking-water, sterilization are isolated the nonreactive cultural method of uniting with bacterium and biology, it is characterized in that comprising following four aspects:
(1) according to adding the standard of 2～4kg feed in the feed per ton with bacterial classification; In feed, add feed and use bacterial classification; Wherein, It is 50～10,000,000,000 viable bacterias/g that feed uses the bacterial content of bacterial classification, and according to the parts by weight meter, feed is formulated by 2～3 parts of bacillus licheniformis and 1 part of bacillus subtilis with bacterial classification;
(2) the drinking water bacterial classification is processed the drinking water bacteria suspension that concentration is 50～10,000,000,000 viable bacterias/mL; The drinking water bacteria suspension compares thin up according to 1: 80～160 quality then; Maintain the standard of 1.5～300,000,000 viable bacterias/kg according to beneficial bacterium valid density in the animal body; In the daily drinking water of biology, add the drinking water bacteria suspension; Wherein, according to the parts by weight meter, the drinking water bacterial classification is formulated by 2～3 parts of lactobacillus acidophilus, 2～3 parts of bacillus licheniformis, 1 part of bacillus subtilis, 2～3 portions of candida tropicalises, 1～2 part of bafillus natto, 1 part of streptococcus fecalis and 1～2 part of Bifidobacterium;
(3) will sterilize that to use bacterial classification to process concentration be that bacteria suspension use in the sterilization of 50～10,000,000,000 viable bacterias/mL, then sterilization with bacteria suspension according to 1: 50～100 quality than thin up, spray disinfectant in feeding environment wherein, is sterilized with bacterial classification employing EM bacterium at last;
(4) plantation landscape tree and the Chinese herbal medicine with disinfective action replace the isolation strip of adjacent turn my humble abode.
2. feed according to claim 1, drinking-water, sterilization be with bacterium and biological nonreactive cultural method of isolating associating, it is characterized in that landscape tree in the aspect (4) is preferably one or more in Chinese wax, method paulownia or the Chinese scholar tree.
3. feed according to claim 1, drinking-water, sterilization is characterized in that with bacterium and biological nonreactive cultural method of isolating associating the Chinese herbal medicine in the aspect (4) is preferably honeysuckle.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104178433A (en) * | 2014-09-04 | 2014-12-03 | 山东省农业科学院生物技术研究中心 | Compound microbial preparation for treating urban river water pollution and preparation method of compound microbial preparation |
| CN107691348A (en) * | 2017-09-19 | 2018-02-16 | 郝天伟 | A kind of cultural method of low fat reed catkins chicken |
| CN108112541A (en) * | 2017-12-26 | 2018-06-05 | 杨凌欣益生态农业科技发展有限公司 | A kind of cultural method of livestock and poultry cultivation zero-emission |
| CN109496981A (en) * | 2018-12-27 | 2019-03-22 | 北京市畜牧总站 | A method of for improving Egg Production of Laying Hens quality |
| CN109601446A (en) * | 2018-11-23 | 2019-04-12 | 王熙涛 | A kind of preparation and application of the dedicated micro- raw first preparation of the steady water of prawn culturing |
| CN110419643A (en) * | 2019-09-04 | 2019-11-08 | 刘泳君 | Fermented tcm preparation and the preparation method and application thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104178433A (en) * | 2014-09-04 | 2014-12-03 | 山东省农业科学院生物技术研究中心 | Compound microbial preparation for treating urban river water pollution and preparation method of compound microbial preparation |
| CN107691348A (en) * | 2017-09-19 | 2018-02-16 | 郝天伟 | A kind of cultural method of low fat reed catkins chicken |
| CN108112541A (en) * | 2017-12-26 | 2018-06-05 | 杨凌欣益生态农业科技发展有限公司 | A kind of cultural method of livestock and poultry cultivation zero-emission |
| CN109601446A (en) * | 2018-11-23 | 2019-04-12 | 王熙涛 | A kind of preparation and application of the dedicated micro- raw first preparation of the steady water of prawn culturing |
| CN109496981A (en) * | 2018-12-27 | 2019-03-22 | 北京市畜牧总站 | A method of for improving Egg Production of Laying Hens quality |
| CN110419643A (en) * | 2019-09-04 | 2019-11-08 | 刘泳君 | Fermented tcm preparation and the preparation method and application thereof |
| CN110419643B (en) * | 2019-09-04 | 2023-05-23 | 刘泳君 | Fermented traditional Chinese medicine preparation and preparation method and application thereof |
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