CN102362844B - Aromatic plant bacteriostatic skin-care foot washing liquid and preparation method thereof - Google Patents

Abstract

The invention relates to an antibacterial skin care washing liquid in the field of traditional Chinese medicine, in particular to an aromatic plant antibacterial skin care foot washing liquid and a preparation method thereof. The invention aims at treating beriberi, the disadvantages of large side effects of western medicine and inconvenient use of traditional Chinese medicine, and provides an aromatic plant antibacterial skin care footwash and a preparation method thereof which has good antibacterial effect and is convenient to use. The main components of the antibacterial skin care washing liquid include, in parts by weight: 1-15 parts of white fresh bark and Sophora flavescens extract, 1-15 parts of aloe extract, 7-12 parts of ammonium lauryl polyoxyethylene ether sulfate, twelve 5-10 parts of alkyl sodium sulfate, 0.1-0.2 parts of EDTA-2Na, 0.05-0.1 parts of benzoic acid, 1-5 parts of salicylic acid, white fresh bark and flavescens in an equal weight ratio.

Description

Aromatic plant antibacterial skin care footwash and preparation method thereof

technical field

The invention relates to an antibacterial skin care washing liquid in the field of traditional Chinese medicine, in particular to an aromatic plant antibacterial skin care foot washing liquid and a preparation method thereof.

Background technique

Beriberi is a common skin disease, and the incidence of beriberi in my country is about 10%. There are many patients with this disease, which is universal, multiple, and easy to relapse. Beriberi is caused by dermatophytes (mainly fungi such as Trichophyton rubrum, Trichophyton mentagrophytes and Epidermophyton flocculum). At present, antifungal ointment drugs are mostly used in clinical Western medicine treatment. Although these drugs have good curative effects, they have many adverse reactions and are prone to drug resistance. Clinically, traditional Chinese medicine treats beriberi with traditional Chinese medicine water decoctions for soaking and washing. Although these water decoctions have good curative effect, they need to be decocted with fire before use, which brings great trouble to patients and is also inconvenient for transportation. Modern research on Chinese herbal medicine provides a certain basis for the further development of anti-dermatophyte effects of Chinese herbal medicine. Modern research shows that Sophora flavescens has obvious inhibitory effects on superficial fungi such as Trichophyton rubrum, Trichophyton gypsum, and Microsporum gypsum. Antibacterial effect, white fresh skin has inhibitory effect on a variety of pathogenic fungi, and has a certain effect on skin suppurative ulcers. Aloe vera has the functions of sterilization, anti-ulcer, promotion of wound healing, skin care, deodorant, dekeratinization, and softening of scars.

With the enhancement of people's health care awareness and the pursuit of health concepts, disinfection and sterilization hand sanitizers are popular among people, but this type of hand sanitizers is more focused on inhibiting bacteria, and has weak or even no inhibitory effect on fungi that cause beriberi.

Contents of the invention

The invention aims at treating beriberi, the disadvantages of large side effects of western medicine and inconvenient use of traditional Chinese medicine, and provides an aromatic plant antibacterial skin care footwash and a preparation method thereof which has good antibacterial effect and is convenient to use.

The present invention combines clinical and folk experience, screens out traditional Chinese medicine Sophorae flavescens and white fresh skin with good curative effect and strong antibacterial effect as main raw materials, adds skin care ingredients aloe extract, aromatic essential oil bergamot oil with transdermal absorption promoting effect, and Decontamination, emulsifying surfactants, etc., according to the preparation method of detergents, are developed into footwashes with antibacterial and skin care effects, which overcome the disadvantages of Western medicines that are prone to drug resistance and certain toxic and side effects, and satisfy consumers. Green products and nutrition, safe consumer psychology.

An aromatic plant antibacterial skin care footwash, the components of which include in parts by weight: 1-15 parts of white fresh bark and Sophora flavescens extract, 1-15 parts of aloe extract, lauryl polyoxyethylene ether ammonium sulfate 7 to 12 parts, 5 to 10 parts of sodium lauryl sulfate, 0.1 to 0.2 parts of EDTA-2Na, 0.05 to 0.1 parts of benzoic acid, 1 to 5 parts of salicylic acid, white fresh skin and flavescens in equal weight Compare. White fresh bark and Sophora flavescens extracts mainly have antibacterial, fungal and anti-inflammatory effects; aloe vera extracts mainly have skin care effects; ammonium lauryl polyoxyethylene ether sulfate is a detergent and foaming agent; Pollution, emulsification and foaming; salicylic acid and benzoic acid have antifungal, antiseptic, and keratin-dissolving effects.

The above-mentioned aromatic plant antibacterial skin care footwash, preferably, its components also include: 2-8 parts of ethylene glycol distearate. Ethylene glycol distearate produces a pearlescent effect.

The above-mentioned aromatic plant antibacterial skin care footwash, preferably, its components also include: 2-10 parts of alkanolamide. Alkanolamide nonionic surfactant that acts as a thickener and foam stabilizer.

The above-mentioned aromatic plant antibacterial skin care footwash, as a preference, its components also include: 3-15 parts of cocamidopropyl betaine in 35% by weight. Cocamidopropyl betaine is a zwitterionic surfactant with less irritation, more foam and strong detergency.

The above-mentioned aromatic plant antibacterial skin care footwash, as preferably, the pH of the solution is adjusted to 6.0 to 6.8 with citric acid.

The above-mentioned aromatic plant antibacterial skin care footwash, preferably, its components also include: 0.1 to 5 parts of bergamot oil. Bergamot oil has the effect of promoting transdermal absorption; its aromatic smell can improve foot odor.

The above-mentioned aromatic plant antibacterial skin care footwash, as a preference, its components also include: indigo. Indigo has a toning effect.

In terms of weight percentage, the components of the aromatic plant antibacterial skin care footwash are: 1-15% of white fresh bark and Sophora flavescens extract; 1-15% of aloe extract; 7-12% of ammonium laureth polyoxyethylene ether sulfate %; Sodium Lauryl Sulfate 5-10%; 35% Cocamidopropyl Betaine Solution 3-15%; Alkanolamide 2-10%; Ethylene Glycol Distearate 2-8%; EDTA -2Na 0.1-0.2%, salicylic acid 1-5%, benzoic acid 0.05-0.1%, indigo 0.01-0.05%, bergamot oil 0.1-5%, the rest is citric acid solution, the citric acid solution is adjusted to PH to 6.0 ~6.8.

In conclusion, the present invention solves the following problems. (1) Aiming at the problem that beriberi is caused by infection of fungi: the traditional Chinese medicine Sophora flavescens and white fresh cortex, which have good curative effect and strong antibacterial effect and are commonly used in the treatment of beriberi, are used as the main raw materials in the prescription. Ginseng extract has antifungal effect. (2) For the symptoms of inflammation, itching, and injury that often occur on the feet of patients: the white fresh skin, flavescens and aloe in the prescription have anti-inflammatory and anti-itching effects, which can relieve the patient's itching and unbearable pain. (3) For the problem of recurrent beriberi: the essential oil bergamot oil in the prescription can promote the transdermal absorption of drugs, and its aromatic smell can also improve the situation of patients with beriberi. (4) For the characteristics of pathogenic fungi that love alkali and fear acid: the salicylic acid in the prescription has antifungal, antiseptic, and keratin-dissolving effects. Facilitate drug penetration. (5) In order to make the footwash liquid have a mild decontamination effect: this recipe uses ammonium lauryl alcohol polyoxyethylene ether ammonium sulfate, which is less irritating, mild in performance, has no decomposition effect on antibacterial components, and has sufficient decontamination performance. Surfactants such as cocamidopropyl betaine.

The method for the aromatic plant antibacterial skin care foot washing liquid of the present invention, the steps comprise:

(1) Add 5 to 10 times the amount of 95% ethanol to the medicinal materials of Sophora flavescens and white fresh skin, heat and reflux to extract 2 to 3 times, each time for half an hour, filter, and concentrate the filtrate to a thick extract to obtain Sophora flavescens and white fresh skin fresh bark extract;

(2) Heat ethylene glycol distearate to 75-80°C and melt to obtain liquid A;

(3) Dissolve EDTA-2Na in deionized water, heat to 75-80°C, add ammonium lauryl polyoxyethylene ether sulfate and sodium lauryl sulfate, stir and dissolve to obtain liquid B;

(4) Add liquid A heated to 75-80°C to liquid B at the same temperature, stir while adding, and mix well to obtain liquid C;

(5) Add Sophora flavescens and white fresh bark extract and aloe extract, salicylic acid, benzoic acid to C liquid, stir and mix;

(6) Add 35% cocamidopropyl betaine solution and alkanolamide, stir and mix. Then add citric acid to adjust the pH value to 6.0-6.8;

The preparation method of the above-mentioned aromatic plant antibacterial skin care footwash liquid, as a preference, also includes step (7) adding bergamot oil at the end, stirring and mixing.

The preparation method of above-mentioned aromatic plant antibacterial skin care footwash, as preferably, in the step (5), add indigo simultaneously.

The main effect of the invention is that the foot washing liquid has the advantages of decontamination, antibacterial, skin care, etc., is simple to make, low in cost, easy to use, and convenient to carry.

Detailed ways

Below in conjunction with embodiment: Example, the present invention is further described.

Example 1:

Aromatic plant antibacterial skin care foot wash raw materials: 10 grams of white fresh skin and Sophora flavescens extract; 10 grams of aloe vera extract; 10 grams of ammonium lauryl polyoxyethylene ether sulfate; 7 grams of sodium lauryl sulfate; 35% Cocamidopropyl betaine solution 14g; alkanolamide 2g; ethylene glycol distearate 4g; EDTA-2Na 0.2g; salicylic acid 1g; benzoic acid 0.1g; indigo 0.01g; Bergamot oil 0.5 grams. The white fresh skin and Sophora flavescens are in an equal weight ratio of 1:1.

According to the above-mentioned raw materials, the preparation method of aromatic plant antibacterial skin care footwash:

(1) Add 5 to 10 times the amount of 95% ethanol to the medicinal materials of Sophora flavescens and white fresh skin, heat and reflux to extract 2 to 3 times, each time for half an hour, filter, and concentrate the filtrate to a thick extract to obtain Sophora flavescens and white fresh skin fresh bark extract;

(2) Heat ethylene glycol distearate to 75-80°C and melt to obtain liquid A;

(3) Dissolve EDTA-2Na in deionized water, heat to 75-80°C, add ammonium lauryl polyoxyethylene ether sulfate and sodium lauryl sulfate, stir and dissolve to obtain liquid B;

(4) Add liquid A heated to 75-80°C to liquid B at the same temperature, stir while adding, and mix well to obtain liquid C;

(5) Add Sophora flavescens and white fresh bark extract and aloe extract, salicylic acid, benzoic acid, indigo to C liquid, stir and mix;

(6) Add 35% cocamidopropyl betaine solution and alkanolamide, stir and mix. Then add citric acid to adjust the pH value to 6.0-6.8;

(7) Finally, add bergamot oil, stir and mix well.

Example 2:

Aromatic plant antibacterial skin care foot wash raw materials: white fresh skin and Sophora flavescens extract 1g; aloe extract 1g; ammonium lauryl polyoxyethylene ether sulfate 7g; sodium lauryl sulfate 5g; 35% 3 grams of cocamidopropyl betaine solution; 2 grams of alkanolamide; 2 grams of ethylene glycol distearate; 0.1 grams of EDTA-2Na; 1 gram of salicylic acid; 0.05 grams of benzoic acid; 0.01 grams of indigo; Bergamot oil 0.5 grams.

The preparation method is the same as in Example 1.

Example 3:

Aromatic plant antibacterial skin care foot wash raw materials: 15 grams of white fresh skin and Sophora flavescens extract; 15 grams of aloe extract; 12 grams of ammonium lauryl polyoxyethylene ether sulfate; 10 grams of sodium lauryl sulfate; 35% 15 grams of cocamidopropyl betaine solution; 10 grams of alkanolamide; 8 grams of ethylene glycol distearate; 0.2 grams of EDTA-2Na; 5 grams of salicylic acid; 0.1 grams of benzoic acid; 0.01 grams of indigo; Bergamot oil 0.5 grams.

The preparation method is the same as in Example 1.

Example 4:

Aromatic plant antibacterial skin care foot wash raw materials: 5 grams of white fresh skin and Sophora flavescens extract; 5 grams of aloe extract; 7 grams of ammonium lauryl polyoxyethylene ether sulfate; 6 grams of sodium lauryl sulfate; 35% 15 grams of cocamidopropyl betaine solution; 10 grams of alkanolamide; 8 grams of ethylene glycol distearate; 0.2 grams of EDTA-2Na; 5 grams of salicylic acid; 0.1 grams of benzoic acid; 0.01 grams of indigo; Bergamot oil 0.5 grams.

The preparation method is the same as in Example 1.

Experimental example 1:

According to the preparation method, the plant antibacterial skin care footwash is developed into a grass-green viscous liquid in appearance, fine and uniform in texture, free of foreign body sensation, and has a slight fragrance of bergamot oil.

After stirring, the foam height was measured to be 245mm (40°C).

Experimental example 2:

Stability test (cold resistance and heat resistance test): put the footwash liquid in a refrigerator at (-5±2)°C for 24 hours, take it out and observe it when it returns to room temperature, there is no precipitation and discoloration, and the transparent product is not cloudy; at (40±2) 1) Put it in an incubator at ℃ for 24 hours, take it out and observe it when it returns to room temperature, there is no peculiar smell, no delamination and discoloration.

Experimental example 3:

The evaluation method of antibacterial effect of footwash is based on QB/T 2738-2005 Antibacterial effect test method of antibacterial daily chemical products.

Antibacterial ring method, the specific operation is as follows:

(1) Preparation of Antibacterial Tablets

Take four pieces of sterile dry filter paper, add 20 μL of footwash solution dropwise to each piece, then place the filter paper piece flat in a clean sterile plate, let it dry naturally at room temperature before use;

(2) Preparation of control samples

Take sterile dry filter paper pieces, add 20 μL of sterile distilled water dropwise to each piece, dry and set aside;

(3) Inoculation of test bacteria

Use a sterile cotton swab to dip the test bacteria suspension with a concentration of 1×10 ⁴ to 9×10 ⁴ cfU/mL, and evenly spread it on the surface of the nutrient agar medium plate for 3 times. Every time you apply it, the tablet should be rotated 60 degrees, and finally use a cotton swab to apply it around the edge of the tablet for a week. Cover the plate and let it dry at room temperature for 5 min.

Use sterile tweezers to stick 4 pieces of test samples and 1 piece of control sample on the surface of each plate. The distance between the centers of each sample is more than 25mm, and the distance between the center of each plate is more than 15mm. After pasting, use sterile tweezers to gently press the sample to make it stick to the surface of the plate. Cover the plate well, and when the test bacteria are coliform bacteria, Staphylococcus aureus or Pseudomonas aeruginosa, culture in a 37°C incubator for 16-18 hours; In the case of ringworm, Microsporum gypsumoidus or Microsporum canis, culture it in an incubator at 26°C for 7 days, and then observe the results. The diameter of the inhibition zone was measured with a vernier caliper, and the experiment was repeated three times.

The results showed that the antibacterial ring diameters of the footwash to Trichophyton rubrum, Trichophyton mentagrophytes, Epidermophyton flocculus, Microsporum gypsumoid and Microsporum canis were significantly greater than 7mm; , Staphylococcus aureus, and Pseudomonas aeruginosa had a diameter of inhibition zone greater than 7mm.

According to QB/T 2738-2005 Inspection and evaluation of antibacterial effect of antibacterial daily chemical products: if the diameter of the antibacterial ring is greater than 7mm, it belongs to the level above B2.

Experimental example 4:

The evaluation method of antibacterial effect of footwash is based on QB/T 2738-2005 Antibacterial effect test method of antibacterial daily chemical products.

Suspension quantitative method, the specific operation is as follows:

(1) Take the stock solution of footwash liquid and put it into a 5.0mL sterilized test tube, keep the temperature at 20°C for 5min. Draw 0.1mL of the test bacteria solution (concentration is about 1×10 ⁴ ~9×10 ⁴ cfU/mL) and add it to the footwash sample solution, mix quickly, and immediately count the time. After 30 minutes of action, take the test bacteria and the sample Add 1 mL of the mixture to a test tube containing 5.0 mL of sterilized PBS, and mix well. After standing for 10 minutes, draw 1mL of the sample solution after mixing, place it in a sterilized plate, and inoculate two sterilized plates with each sample solution. Pour 15mL of medium cooled to 40°C-45°C, rotate the plate to make it fully uniform, turn over the plate after the agar solidifies, and place it at 37°C when the test bacteria are coliform bacteria, Staphylococcus aureus or Pseudomonas aeruginosa Cultivate in an incubator for 16 to 18 hours; when the test bacteria are Trichophyton rubrum, Trichophyton mentagrophytes, Epidermophyton flocculus, Microsporum gypsumiformis or Microsporum canis, culture them in a 26°C incubator for 7 days, and do live Bacterial colony count;

(2) Replace the test sample with PBS, and operate according to the above steps at the same time, as a control sample; repeat the experiment three times, and calculate the average value.

Bacterial inhibition rate = (average number of colonies in control sample - average number of colonies in test sample) ÷ average number of colonies in control sample

As a result, the footwash liquid was effective against Trichophyton rubrum, Trichophyton mentagrophytes, Epidermophyton flocculus, Microsporum gypsum, Microsporum canis, coliform bacteria, Staphylococcus aureus, and Pseudomonas aeruginosa. Bacteria rates were greater than 90%.

According to literature reports, Trichophyton rubrum, Trichophyton mentagrophytes, Epidermophyton flocculus, Microsporum gypsumoides and Microsporum canis are the main dermatophytes causing beriberi infection. The antibacterial ring method and suspension quantitative method antibacterial test results show that the footwash has obvious inhibitory effect on the main fungi that cause beriberi infection. Bacteria also have an inhibitory effect.

Claims (1)

1. an aromatic type plant antibacterial skin care footwash, is characterized in that, its component is by weight percentage: white fresh bark and Sophora flavescens extract 1～15%, aloe extract 1～15%, lauryl polymer Oxyethylene ether ammonium sulfate 7-12%, sodium lauryl sulfate 5-10%, ETA-2Na 0.1-0.2%, benzoic acid 0.05-0.1%, salicylic acid 1-5%, distearic acid Ethylene glycol ester 2-8%, alkanolamide 2-10%, 35% cocamidopropyl betaine solution 3-15%, bergamot oil 0.1-5%, indigo 0.01-0.05%, the balance is citric acid solution, adjust the pH value to 6.0-6.8 with citric acid, Among them, the extracts of Sophora flavescens and Sophora flavescens are prepared by the following process: adding equal weight of Sophora flavescens and Sophora flavescens to 95% ethanol of 5 to 10 times the weight, heating and refluxing for 2 to 3 times, each half Hours, filtered, combined filtrates, concentrated to extract, that is. the