CN102283793A - Traditional Chinese medicine compound with whitening function, and preparation method and application thereof - Google Patents
Traditional Chinese medicine compound with whitening function, and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine compound with a whitening function, a preparation method thereof and application thereof. The traditional Chinese medicine compound is prepared from the following raw medicinal materials in part by weight: 3 to 20 parts of Chinese gall carbon, 3 to 10 parts of phellodendron amurense and 3 to 10 parts of sinkiang arnebia root. The traditional Chinese medicine compound provided by the invention is prepared from traditional Chinese medicines in proportion by adopting treatment based on syndrome differentiation according to the theory of traditional Chinese medicine. An experimental result shows that: the traditional Chinese medicine compound provided by the invention has the effects of well inhibiting the activity of tyramine oxidase, inhibiting the growth of B16F10 cells, inhibiting the proliferation of B16F10 cells and promoting the apoptosis of B16F10 cells and has a definite whitening function. And the extract of the traditional Chinese medicine compound is safely used, has no toxic and side effects, has stable quality, can be prepared into a plurality of articles for daily use and is prospected to be developed into a new generation of whitening preparation.
Description
Technical field
The present invention relates to a kind of Chinese medicine compound, be specifically related to a kind of Chinese medicine compound and preparation method thereof and its application of Pear Power function.
Background technology
The color of skin depends primarily in the skin contained how much melanic.Melanin is generated by the melanocyte in the epidermis, and its synthetic influence that is subjected to plurality of enzymes in the cell comprises tryrosinase, dopachrome isomerase, 5, and 6-two is through oxyindole-2-carboxylic acid (DHICA) oxidase etc.Wherein tryrosinase is main rate-limiting enzyme, and this enzymatic activity size is determining the quantity that melanin forms.In addition, the melanic synthetic influence that also is subjected to factors such as ultraviolet, free radical.
Current whitening product great majority are based on restraint of tyrosinase.These existing medicaments, also unsatisfactory at aspects such as stability, side effect, effects.For example, the hydroquinone use that is under an embargo owing to side effect is excessive, kojic acid and derivant whitening function thereof are strong, but meet light, heat is easily decomposed, and causes the skin pigment deposition easily after the preparation of whitening that contains chemical constituent or heavy metal uses, side effect is bigger.The natural product of plant amedica is used for cosmetics can play good treatment and skin protection double effects, and have function many, widely applicable, safe, have no side effect or characteristic such as side effect is little substantially, thereby in the day chemical industry, more and more paid attention to, have a extensive future.
Summary of the invention
Goal of the invention: the objective of the invention is to solve the deficiencies in the prior art, provide a kind of proportioning scientific and reasonable, whitening effect is definite, clinical Chinese medicine compound safe in utilization, another object of the present invention provide the preparation method of this Chinese medicine compound and whiten in preparation, the application in the anti-acne article of everyday use.
Technical scheme: in order to realize above purpose, the technical scheme that the present invention takes is:
A kind of Chinese medicine compound of Pear Power function, it is to be made by the crude drug of following parts by weight: 3～10 parts of Galla Chinensis charcoals, 3～10 parts of Cortex Phellodendris, 3～10 parts of Radix Arnebiae (Radix Lithospermi)s.
As preferred version, the Chinese medicine compound of above-described Pear Power function, it is to be made by the crude drug of following parts by weight: 3～6 parts of Galla Chinensis charcoals, 3～6 parts of Cortex Phellodendris, 3～6 parts of Radix Arnebiae (Radix Lithospermi)s.
As more preferably scheme, the Chinese medicine compound of above-described Pear Power function, it is to be made by the crude drug of following parts by weight: 3 parts of Galla Chinensis charcoals, 3 parts of Cortex Phellodendris, 3 parts of Radix Arnebiae (Radix Lithospermi)s.
Above-described Galla Chinensis charcoal is fried to concoct by Galla Chinensis carbon and was obtained in 20～30 minutes.
The Chinese medicine compound of Pear Power function provided by the invention, can be with acceptable carrier on each extracts of bulk drugs and the article of everyday use, be prepared into ointment, ointment, facial cream, gel, emulsion, spray or cleansing milk as matrix components such as wetting agent, antioxidant, surfactant, alcohols, water, as other preferred version, also can be prepared into powder, fancy soap, oil, dry powder, foundation emulsion or wet-milling etc., the convenient use.
The preparation method of the Chinese medicine compound of Pear Power function provided by the invention, it may further comprise the steps:
At first get Galla Chinensis carbon and fried 20～30 minutes, get Galla Chinensis carbon, get Galla Chinensis carbon, Cortex Phellodendri and Radix Arnebiae (Radix Lithospermi) by weight and beat fine powder, standby, add the extracting solution reflux, extract, then 1 to 3 time, refluxed merge extractive liquid, at every turn 1 to 3 hour, filter, get filtrate, concentrated medicament adds ethanol precipitation, get filtrate, concentrate promptly.
As preferred version, the preparation method of the Chinese medicine compound of above-described Pear Power function, described extracting solution is water, methanol, ethanol, aqueous alcohols or acetone etc.
As preferred version, the preparation method of the Chinese medicine compound of above-described Pear Power function, the extraction liquid measure that adds during reflux, extract, are 6 to 10 times of medical material amount.
As preferred version, the preparation method of the Chinese medicine compound of above-described Pear Power function, it is 70～80% that adding ethanol makes concentration of ethanol, can remove a large amount of macromole impurity, improves clinical efficacy.
As another preferred version, the preparation method of the Chinese medicine compound of above-described Pear Power function obtains also can removing impurity by resin column behind the extracting solution, obtains purified extract.
The Chinese medicine compound of Pear Power function provided by the invention is whitened in preparation, the application in the anti-acne article of everyday use.
Beneficial effect: the present invention is according to Chinese medical theory, adopt the theory of determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs to carry out the Chinese prescription screening, the pharmaceutical composition proportioning that provides is scientific and reasonable, clinical experiment is the result show, Chinese medicine compound provided by the invention has good restraining tryrosinase, free radical resisting and anti-ultraviolet effect, can effectively suppress melanin and generate, desalination pigmentation, senile plaque, freckle etc.Compare with the whitening agent of prior art, the Chinese medicine compound that invention provides can suppress melanic synthetic from different approach comprehensively, has excellent whitening effect, simultaneously, this Chinese medicine compound contains the plurality of Chinese active component, do not contain the chemosynthesis material, can promote the metabolism of skin, have no side effect, little to skin irritation, the nutrition that can improve skin makes skin pure white naturally tender with healthy, and stable in properties.
The preparation method of while Chinese medicine compound provided by the invention, process stabilizing, effective component extracting content height, impurity is few, and is workable, is fit to industrialized great production.
Description of drawings
Fig. 1 is the light microscopic figure of the traditional Chinese medicine compound extract of variable concentrations to the influence of B16F10 cellular morphology.
The specific embodiment:
Further illustrate the present invention below in conjunction with specific embodiment, should understand these embodiment only is used to the present invention is described and is not used in and limit the scope of the invention, after having read the present invention, those skilled in the art all fall within the application's claims institute restricted portion to the modification of the various equivalent form of values of the present invention.
Embodiment 1
1, the crude drug of the Chinese medicine compound of Pear Power function: 3 parts of Galla Chinensis charcoals, 3 parts of Cortex Phellodendris, 3 parts of Radix Arnebiae (Radix Lithospermi)s.
2, the preparation method of the Chinese medicine compound of Pear Power function:
At first getting Galla Chinensis carbon fried 20～30 minutes, get Galla Chinensis carbon, get 3 parts in Galla Chinensis carbon, 3 parts of Cortex Phellodendris and Radix Arnebiae (Radix Lithospermi) spend the night in oven drying for 3 parts, pulverize the back and cross 40 mesh sieves, standby, add the water of 7 times of medical material amounts, heating and refluxing extraction 1 hour then, filter, filtering residue adds the water of 3 times of amounts again, heating and refluxing extraction 1 hour, merge extractive liquid,, filter, get filtrate, concentrated medicament, it is 70% that the ethanol of adding 95% makes concentration of alcohol, leaving standstill forms sediment sinks, get filtrate, concentrate, add the active carbon of 0.2%-0.3%, 80～90 ℃ of heating 2 hours, filter and obtain Chinese medicine extract and wetting agent, antioxidant, surfactant, alcohols, matrix components such as water are prepared into ointment, ointment, facial cream, gel, emulsion, spray or cleansing milk.(lot number: 001)
Embodiment 2
1, the crude drug of the Chinese medicine compound of Pear Power function: 10 parts of Galla Chinensis charcoals, 10 parts of Cortex Phellodendris, 10 parts of Radix Arnebiae (Radix Lithospermi)s.
2, the preparation method of the Chinese medicine compound of Pear Power function:
At first getting Galla Chinensis carbon fried 20～30 minutes, get Galla Chinensis carbon, get 10 parts in Galla Chinensis carbon, 10 parts of Cortex Phellodendris and Radix Arnebiae (Radix Lithospermi) spend the night in oven drying for 10 parts, pulverize the back and cross 40 mesh sieves, standby, add the water of 8 times of medical material amounts, heating and refluxing extraction 2 hours then, filter, filtering residue adds the water of 6 times of amounts again, heating and refluxing extraction 1 hour, merge extractive liquid,, filter, get filtrate, concentrated medicament, it is 80% that the ethanol of adding 95% makes concentration of alcohol, leaving standstill forms sediment sinks, get filtrate, concentrate, add the active carbon of 0.2%-0.3%, 80～90 ℃ of heating 2 hours, filter and obtain Chinese medicine extract and wetting agent, antioxidant, surfactant, alcohols, matrix components such as water are prepared into ointment, ointment, facial cream, gel, emulsion, spray or cleansing milk.(lot number: 002)
Embodiment 3
1, the crude drug of the Chinese medicine compound of Pear Power function: 6 parts of Galla Chinensis charcoals, 6 parts of Cortex Phellodendris, 6 parts of Radix Arnebiae (Radix Lithospermi)s.
2, the preparation method of the Chinese medicine compound of Pear Power function:
At first getting Galla Chinensis carbon fried 20～30 minutes, get Galla Chinensis carbon, get 6 parts in Galla Chinensis carbon, 6 parts of Cortex Phellodendris and Radix Arnebiae (Radix Lithospermi) spend the night in oven drying for 6 parts, pulverize the back and cross 40 mesh sieves, standby, add the water of 10 times of medical material amounts, heating and refluxing extraction 1 hour then, filter, filtering residue adds the water of 6 times of amounts again, heating and refluxing extraction 2 hours, merge extractive liquid,, filter, get filtrate, concentrated medicament, it is 70% that the ethanol of adding 95% makes concentration of alcohol, leaving standstill forms sediment sinks, get filtrate, concentrate, add the active carbon of 0.2%-0.3%, 80～90 ℃ of heating 2 hours, filter and obtain Chinese medicine extract and wetting agent, antioxidant, surfactant, alcohols, matrix components such as water are prepared into ointment, ointment, facial cream, gel, emulsion, spray or cleansing milk.(lot number: 003)
Embodiment 4
1, the crude drug of the Chinese medicine compound of Pear Power function: 8 parts of Galla Chinensis charcoals, 4 parts of Cortex Phellodendris, 6 parts of Radix Arnebiae (Radix Lithospermi)s.
2, the preparation method of the Chinese medicine compound of Pear Power function:
At first getting Galla Chinensis carbon fried 20～30 minutes, get Galla Chinensis carbon, get 8 parts in Galla Chinensis carbon, 4 parts of Cortex Phellodendris and Radix Arnebiae (Radix Lithospermi) spend the night in oven drying for 6 parts, pulverize the back and cross 40 mesh sieves, standby, add the water of 8 times of medical material amounts, heating and refluxing extraction 2 hours then, filter, filtering residue adds the water of 6 times of amounts again, heating and refluxing extraction 1 hour, merge extractive liquid,, filter, get filtrate, concentrated medicament, it is 75% that the ethanol of adding 95% makes concentration of alcohol, leaving standstill forms sediment sinks, get filtrate, concentrate, add the active carbon of 0.2%-0.3%, 80～90 ℃ of heating 2 hours, filter and obtain Chinese medicine extract and wetting agent, antioxidant, surfactant, alcohols, matrix components such as water are prepared into ointment, ointment, facial cream, gel, emulsion, spray or cleansing milk.(lot number: 004)
Embodiment 5 pharmacodynamics evaluations
1, the B16F10 melanoma cells is cultivated and is gone down to posterity
Under the aseptic condition, in the culture bottle of the DMEM culture medium that contains 10% hyclone, at 37 ℃, volume fraction is 5%CO with cell inoculation
2Cultivate in the incubator, change liquid every day, treat that cell fusion reaches 80% and goes down to posterity when above, per 2～3d goes down to posterity 1 time with 0.25% trypsinization, experiment with the 3rd～4 generation cell.Treat that cell grows into logarithmic (log) phase, with PBS washing, 0.25% trypsinization, cell counting count board counting, it is to be measured to be seeded to Tissue Culture Plate then.
2, traditional Chinese medicine compound extract is to the influence of B16F10 cellular morphology
Observation of cell form and growing state under light microscopic, as described in Figure 1, discovery eumelanin oncocyte is fusiformis, and cell space is bigger, and endochylema is abundant, and homogeneous transparent, cell have 2～3 dendroids prominent, and projection is short but thick obvious; B16F10 melanoma cells after Chinese medicine extraction liquid (extracting solution 5 and 10ug/ml that the embodiment 1 prepares) effect slightly is fusiformis, the flat attenuation of cell space, and cell space increases, and endochylema is thin, and part cell dendron is not obvious.When concentration reach (extracting solution 20,40 that embodiment 1 prepares, 80ug/ml) after, the B16F10 melanoma cells is polygon, cell space dwindles, the endochylema transparency descends, and along with the increase of dosage, survivaling cell reduces, and more iuntercellular cell debris occurs.
3, traditional Chinese medicine compound extract is to the influence of B16F10 cell proliferation
By of the influence of MTT experimental observation Chinese medicine compound provided by the invention to B16F10 melanoma cells propagation, the experiment medicine: the traditional Chinese medicine compound extract that the embodiment of the invention 1 prepares is divided into 4 groups, dosage is respectively 5,10,40,80ug/ml, control drug: arbutin, show through the MTT of table 1 experimental result, traditional Chinese medicine compound extract has stronger inhibitory action to the B16F10 cell proliferation, in 5～80ug/ml dosage range, be proportional relation preferably, after the administration after 24 hours, the normal cell group is along with the prolongation of incubation time, cytoactive obviously weakens, and shows that traditional Chinese medicine compound extract is having stronger inhibitory action to melanoma cells.
Table 1 Chinese medicine extraction liquid to the influence of B16F10 cell proliferation (n=5,
)
Compare with the cell matched group
*P＜0.05,
*P＜0.01
4, traditional Chinese medicine compound extract is to the influence of B16F10 cell tyrosinase activity
4.1 experiment medicine: the traditional Chinese medicine compound extract that the embodiment of the invention 2 prepares is divided into 4 groups, and dosage is respectively 5,10,20,40ug/ml, control drug: arbutin.
4.2 experimental procedure: treat that cell fusion reaches 80% when above, conventional trypsinization is handled after-blow and is broken into single cell suspension, divides 3 bottles of adding 50ml cell bottles, cultivated 1 day, the prescription that contains that adds variable concentrations is trained every bottle of 6ml, the medicine final concentration is respectively 10,20,40,80ug/ml, after cultivating 24h, find that the normal group cell is full, the observation of cell growing state is taken the photograph sheet under the light microscopic; Every bottle of cell adds the 2ml0.25% trypsinization, after the cell shrinkage, adds culture fluid 2ml and stops digestion, and piping and druming comes off cell fully and is uniformly distributed in culture fluid, adds 2 centrifuge tubes respectively, is respectively applied for to detect melanocyte and tyrosinase activity.
Centrifuge tube is in 1000r/min, centrifugal 5min, abandon supernatant, add 1%Triton X-100 solution 1ml, put-80 ℃ of frozen 1h rapidly, room temperature thawing is subsequently broken cell fully, and 37 ℃ of pre-temperature backs add 0.1%L-DOPA solution 1ml, 37 ℃ of reaction 1h measure absorbance (A) value immediately in microplate reader 490nm wavelength place.Each concentration is established 5 multiple holes.
4.3 experimental result: the experimental result by table 2 shows, compare with the normal group cell, after the traditional Chinese medicine compound extract effect 24 hours, can be the dose-dependent inhibition tyrosinase activity, experimental result shows significantly restraint of tyrosinase activity of traditional Chinese medicine compound extract provided by the invention, generate thereby can suppress melanocyte, reach the effect of good skin whitening.And the experimental result by table 3 shows, traditional Chinese medicine compound extract provided by the invention and normal group cell are relatively, behind the drug effect 24 hours, can obviously suppress the generation of melanocyte, P＜0.01, and present good dose dependent, and stronger than the activity of arbutin, further proof traditional Chinese medicine compound extract of the present invention has the generation of good restraining melanocyte, can bring into play good white-skinned face function.
Table 2 traditional Chinese medicine compound extract to the influence of B16F10 tyrosinase activity (n=5,
)
Compare with the cell matched group
*P＜0.05,
*P＜0.01
Table 3 traditional Chinese medicine compound extract to the influence of B16F10 melanocyte (n=5,
)
Compare with the cell matched group
*P＜0.05,
*P＜0.01
5, traditional Chinese medicine compound extract is to the apoptotic influence of B16F10
5.1 experiment medicine: the traditional Chinese medicine compound extract that the embodiment of the invention 2 prepares is divided into 4 groups, and dosage is respectively 5,10,20,40ug/ml.
5.2 experimental procedure: the trophophase cell of taking the logarithm, conventional trypsinization is handled after-blow and is broken into single cell suspension, and cell concentration is adjusted to 5*10
4Individual/ml, be inoculated in 6 porocyte culture plates, every hole 3ml, after treating cell attachment, abandoning supernatant adds the culture medium that contains the variable concentrations medicine respectively, every hole 3ml, the medicine final concentration is respectively 5,10, and 20,40ug/ml, after continuing to cultivate 24h, detect the apoptosis situation with flow cytometer, each concentration is established 3 multiple holes.
5.3 experimental result: the experimental result by table 4 shows, traditional Chinese medicine compound extract provided by the invention and normal group cell compare, and drug effect can obviously promote the B16F10 apoptosis after 24 hours, and present good dose dependent, thereby can bring into play good white-skinned face function.
Table 4 Chinese medicine extraction liquid to the apoptotic influence of B16F10 (n=3,
)
Compare with the cell matched group
*P＜0.05,
*P＜0.01
Show by above experimental result, Chinese medicine compound provided by the invention has the good restraining tyrosinase activity, suppresses the generation of B16F10 cell melanocyte, suppresses the B16F10 cell proliferation and promote the apoptotic effect of B16F10, thereby can bring into play good white-skinned face function.
The present invention is according to Chinese medical theory, adopt the theory of determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs to carry out the proportioning prescription, show by above experimental result, Chinese medicine compound provided by the invention has the effect that good restraining melanin generates, have definite white-skinned face function, and the Chinese medicine compound extract does not contain toxicity chemical substance or heavy metal, safe in utilization having no side effect, steady quality is expected to be developed to the preparation of whitening of new generation.
The above only is a preferred implementation of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (9)
1. the Chinese medicine compound of a Pear Power function is characterized in that, it is to be made by the crude drug of following parts by weight: 3 ~ 10 parts of Galla Chinensis charcoals, 3 ~ 10 parts of Cortex Phellodendris, 3 ~ 10 parts of Radix Arnebiae (Radix Lithospermi)s.
2. the Chinese medicine compound of Pear Power function according to claim 1 is characterized in that, it is to be made by the crude drug of following parts by weight: 3 ~ 6 parts of Galla Chinensis charcoals, 3 ~ 6 parts of Cortex Phellodendris, 3 ~ 6 parts of Radix Arnebiae (Radix Lithospermi)s.
3. the Chinese medicine compound of Pear Power function according to claim 2 is characterized in that, it is to be made by the crude drug of following parts by weight: 3 parts of Galla Chinensis charcoals, 3 parts of Cortex Phellodendris, 3 parts of Radix Arnebiae (Radix Lithospermi)s.
4. the Chinese medicine compound of each described Pear Power function of claim 1 to 3 is characterized in that, each extracts of bulk drugs and article of everyday use acceptable carrier are prepared into ointment, emulsifiable paste, facial cream, gel, emulsion, spray or cleansing milk.
5. the preparation method of the Chinese medicine compound of claim 1,2 or 3 described Pear Power functions is characterized in that may further comprise the steps:
At first get Galla Chinensis carbon and fried 20 ~ 30 minutes, get Galla Chinensis carbon, get Galla Chinensis carbon, Cortex Phellodendri and Radix Arnebiae (Radix Lithospermi) by claim 1,2 or 3 described parts by weight and beat fine powder, standby, add the extracting solution reflux, extract, then 1 to 3 time, refluxed merge extractive liquid, at every turn 1 to 3 hour, filter, get filtrate, concentrated medicament adds ethanol precipitation, get filtrate, concentrate promptly.
6. the preparation method of the Chinese medicine compound of Pear Power function according to claim 5 is characterized in that: described extracting solution is water, methanol, ethanol, aqueous alcohols or acetone.
7. the preparation method of the Chinese medicine compound of Pear Power function according to claim 5 is characterized in that: the extraction liquid measure that adds during reflux, extract, is 6 to 10 times of medical material amount.
8. the preparation method of the Chinese medicine compound of Pear Power function according to claim 5 is characterized in that: it is 70 ~ 80% that adding ethanol makes concentration of ethanol.
9. the application of Chinese medicine compound in preparation is whitened in preparation of each described Pear Power function of claim 1 to 3.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105534857A (en) * | 2016-01-07 | 2016-05-04 | 江苏海昇药业有限公司 | Chinese herb and gel skin care product and preparation method and application thereof |
| CN107440950A (en) * | 2016-05-30 | 2017-12-08 | 上海家化联合股份有限公司 | Radix Arnebiae extract and its preparation and application |
| CN108393027A (en) * | 2018-03-22 | 2018-08-14 | 四川美酷投资管理有限公司 | A kind of face cream production system |
| CN109350668A (en) * | 2018-10-16 | 2019-02-19 | 新疆医科大学 | Jieduhuaban paste and its preparation method and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1300623A (en) * | 1999-12-20 | 2001-06-27 | 张发平 | Chinese patent medicine |
| CN1761450A (en) * | 2003-02-13 | 2006-04-19 | 株式会社林原生物化学研究所 | External preparation for skin characterized by containing saccharide derivative of α,α-trehalose |
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2011
- 2011-08-19 CN CN2011102379254A patent/CN102283793B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1300623A (en) * | 1999-12-20 | 2001-06-27 | 张发平 | Chinese patent medicine |
| CN1761450A (en) * | 2003-02-13 | 2006-04-19 | 株式会社林原生物化学研究所 | External preparation for skin characterized by containing saccharide derivative of α,α-trehalose |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105534857A (en) * | 2016-01-07 | 2016-05-04 | 江苏海昇药业有限公司 | Chinese herb and gel skin care product and preparation method and application thereof |
| CN107440950A (en) * | 2016-05-30 | 2017-12-08 | 上海家化联合股份有限公司 | Radix Arnebiae extract and its preparation and application |
| CN108393027A (en) * | 2018-03-22 | 2018-08-14 | 四川美酷投资管理有限公司 | A kind of face cream production system |
| CN109350668A (en) * | 2018-10-16 | 2019-02-19 | 新疆医科大学 | Jieduhuaban paste and its preparation method and application |
| CN109350668B (en) * | 2018-10-16 | 2021-07-06 | 新疆医科大学 | Jieduhuaban paste and its preparation method and application |
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Effective date of registration: 20160412 Address after: Yongjin road high tech Industrial Development Zone, Nanjing city of Jiangsu Province, No. 12 210061 Patentee after: Nanjing Haiyuan Prepared Chinese Crude Drugs Co., Ltd. Address before: Yongjin road high tech Industrial Development Zone, Nanjing city of Jiangsu Province, No. 12 210061 Patentee before: Nanjing Haichang Chinese Medicine Group Co., Ltd. |
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