CN102258810A - Preparation method of adipose tissue breast augmentation material enriched with autologous stem cells - Google Patents
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Abstract
The invention belongs to the biomedical field, and in particular discloses a preparation method of an adipose tissue breast augmentation material enriched with autologous stem cells. The preparation method comprises the following steps: firstly only extracting 5ml of the tissues from a patient; then separating, purifying, culturing and amplifying the tissues in vitro to obtain a large quantity of adipose-derived stem cells (ADSCs); and finally adding the amplified ADSCs to a newly prepared SVF (stroma vascular fraction), and then evenly mixing the SVF with the adipose tissues for breast augmentation implantation. By utilizing the preparation method, quantity of the adipose tissues for preparing the SVF can be reduced during the fat transplantation process so as to make up the disadvantage that autogenous fat breast augmentation can not be realized as a large quantity of the adipose tissues for preparing the SVF can not be obtained due to low body mass index (BMI) of large number of women.
Description
(1) technical field
The invention belongs to biomedical sector, particularly a kind of fatty tissue of enrichment autologous stem cells preparation methods of enlarging the bosom.
(2) background technology
Breast enlargement or breast enlargement art (mammary augumentation) are the modal cosmetic surgerys of plastic surgery, since the birth of 20 beginnings of the century, packing material just becomes focal issue, successively attempted many mamaplasty materials, for example paraffin wax, silicon gel, saline, auto derma fat flap or the like, yet, secular medical practice proves, all types of tissue substitute product more or less have some senses of discomfort and complication (to comprise contracture, distortion, hardening, seepage), this is asked doctor and doctors to bring very big puzzlement.So, have the people to begin to attempt to transplant on the basis of enlarging the bosom at dermis fat flap at the end of the seventies and utilize the autologous fat tissue to enlarge the bosom, it is a kind of art of enlarging the bosom that the lipochondrion at position that fat such as health waist, abdomen, buttocks, lower limb is abundanter is transplanted to chest that autologous fat is enlarged the bosom.Operation will the person of enlarging the bosom itself superabundant fats fine needle sucking-off, activation processing becomes pure lipochondrion, evenly injects the mamaplasty district by miniature bobbin itemize reason and makes it to survive; Do not operate on, not hemorrhage, breast enlargement effect true nature having reached simultaneously the fat-reducing body shaping again, has the effect of fat-reducing breast enlargement.Especially in recent years, increasingly mature along with suction lipectomy utilized autologous fat granule injection augmentation mammaplasty to develop rapidly, became one of at present the most popular breast enlarging method.But at present to fill back liquefaction absorbance too high for fat, can reach more than 70%, and survival rate lower (being lower than 40%) wants to reach lasting plentiful effect and must select that " on a small quantity repeatedly " way is given and asked doctor and doctors to bring greatly trouble for use.
The key component of fatty tissue has adipose cell, adipose stromal cells (ASC), vascular endothelial cell, adventitial cell.Adipose stromal cells (ASC) is counted as the exclusive CFU-GM of fatty tissue, promptly gives birth to fat CFU-GM and blood vessel source CFU-GM, and some adipose stromal cells can be divided into various kinds of cell, and is called as adipose-derived stem cell.Adipose stromal cells plays a significant role for fatty tissue running, and (the about every 2-10 circulation primary of fatty tissue), and provide cell for the next generation.What but liposuction obtained mainly is the scattered composition of fatty tissue, studies show that, the adipose stromal cells quantity that the fatty tissue that obtains liposuction contains only is half of complete fatty tissue content, compared with complete fatty tissue, liposuction gained fatty tissue is a kind of fatty tissue of stem cell source difference, and after the fat transplantation, from micro environment, in the acute stage of tissue repair, damage the performance that relevant growth factor and inflammation-related cytokine and chemokines can influence adipose stromal cells (ASC).Fat transplantation will be experienced adipose cell and blood capillary reorganization.Adipose stromal cells (ASC) is the main effect strength of repairing in the fatty tissue.If adipose stromal cells in the suction fatty tissue (ASC) relative deficiency can influence replacement process, cause postoperative to implant lipoatrophy.This also is that at present simple lipochondrion is transplanted the unendurable reason of the effect place of enlarging the bosom, therefore to seek out the persistent effect of enlarging the bosom, must augment the quantity of adipose stromal cells (ASC) or fat stem cell to the insufficient suction fatty tissue of CFU-GM, to improve the survival rate of transplant fat.
(3) summary of the invention
The present invention is in order to remedy the deficiencies in the prior art, and a kind of persistency fatty tissue strong, that enlarge the bosom the enrichment autologous stem cells effective preparation methods of enlarging the bosom is provided.
The present invention is achieved through the following technical solutions:
A kind of fatty tissue of enrichment autologous stem cells preparation methods of enlarging the bosom mainly comprises the steps:
(1) Wicresoft extracts fatty tissue on one's body the sufferer, through steps such as separation and Extraction, purification, detection, amplifications, obtains the stem cell of high quantity in the GMP laboratory;
(2) general anesthesia is extracted fatty tissue with intubate and negative pressure liposuction machine;
(3) it is centrifugal to get half fatty tissue, and the fractionation of fatty inhalation (inhalatio) obtains the transplant fat tissue, and places in the sterile chamber ice bath to preserve;
(4) get second half fatty tissue centrifugalize fats portion and liquid part, separates wherein SVF with gradient centrifugation with enzyme digestion respectively then, and counting survey activity;
(5) amplification is good fat stem cell adds among the SVF that newly prepares, by 10
7The ratio of/100ml fat is added, and at last SVF and transplant fat is organized mixing.
Described sufferer is a Body Mass Index greater than tens years old healthy population to seven teens of 15, wherein, and on one's body except the athlete of few fat.
Packing material of the present invention mainly is made up of autologous fat tissue and adipose-derived substrate vascular components SVF (stroma vascular fraction), and this technology is called for short cell assist type fat transplantation CELL-ASSISTED LIPOTRANSFER (CAL).SVF is a mixing with cells body, has also comprised stromal cell, the fat stem cell of high concentration, hemocytees such as vascular endothelial cell, leukocyte and erythrocyte.The key of this technology has been to add the SVF composition of the fat stem cell that contains high concentration.The fat of taking out when enlarging the bosom divides two parts, and a part is used for transplanting through cryopreservation after the aseptic process, and another part prepares the SVF cell mass and is used for transplanting with the fatty mixing for preparing by special SVF isolation technics at the GMP laboratory.
SVF can keep the abundant of fat tissue cell, wherein each kind of cell can promote surrounding somatomedin as PDGF, EGF, FGF, VEGF, secretions such as IGF, thus further promote the angiogenesis effect, for the fat of transplanting provides blood and nutrient, improved the survival rate of CFU-GM and fatty tissue at short notice; On the other hand, SVF has immunoregulatory effect, can reduce inflammatory reaction, for the fat survival of transplanting provides an adapt circumstance.
In order to improve the concentration of fat stem cell, we at first only extract the 5ml tissue from the patient, through separation, purification, and cultured and amplified in vitro obtain the fat stem cell of high quantity, add at last in the freshly prepd SVF composition, with the transplanting that is used to enlarge the bosom of fatty tissue mixing.
More excellent scheme of the present invention is:
In the step (1), the amount that Wicresoft extracts fatty tissue is 5ml.
In the step (3), fatty tissue is with the centrifugal 5min of the speed of 500G.
In the step (4), fatty tissue is with the centrifugal 5min of the speed of 300G.
The present invention is by extracting the fatty tissue of trace in advance and obtaining a large amount of fat stem cells, be used to prepare the consumption of SVF fatty tissue in the time of so just can reducing fat transplantation, prepare SVF and can not carry out the drawback that autologous fat is enlarged the bosom because of Body Mass Index (at present less than 17 can't autologous fat enlarge the bosom) on the low side can't obtain a large amount of fatty tissuees to remedy a large amount of women.
(4) specific embodiment
Embodiment:
1. patient's selection: Body Mass Index is greater than 15 the healthy population from tens years old to seven teens (on one's body except the athlete of few fat), and liposuction must be done comprehensive health check-up in previous month, comprised the heart, lung, liver and renal function, routine blood test, four of blood coagulations etc.
Simultaneously, those think that the people who once increases (reaching 200 to 400 milliliters) in a large number should not be selected in, because the injection of a course of treatment is limited to 100 to 200 milliliters.
2. the amplification in vitro of fat stem cell
(1) Wicresoft's liposuction, the local anesthesia of injection lignocaine small size utilizes the aseptic extraction of miniature attraction pin patient 5ml fatty tissue;
(2) extraction of fat mesenchymal stem cell: adopt phosphate buffer to soak, 1500r/min centrifuge washing fat extremely, with 37 ℃ of about 30min of water-bath digestion of 0.1% I Collagen Type VI enzyme, add phosphate buffer dilution collagenase and centrifugal, the suspension lower sediment, 100 mesh filter screens filter, the centrifugal 10min of 1500r/m, drip to remove the adipose cell and the fat that suspend, abandon supernatant, carry out In vitro culture subsequently, cultivating system is a STEMPRO MSC SFM serum-free medium, contains the glutamine of 2mM/ml and green grass or young crops-streptomycin of 100U/ml.2 weeks can obtain 10
8-10
9Cell.
3. liposuction
Anaesthetizing sb. generally with being furnished with 0.001% adrenergic saline solution, wait medicine to be penetrated into and get the fat position, is 2.5 millimeters intubate and negative pressure fat suction machine absorption fatty tissue with internal diameter.Extract 800 to 1000 milliliters of fatty tissuees altogether.
4. the preparation of graft materials
(1) preparation of transplant fat tissue: get half and extract thing with the centrifugal 5 min separation of the speed of 300G liposuction tissue, obtain upper strata fractionation of fatty part (PLA) and liquid part (LAF) respectively, the fatty tissue after the separation (PLA) is put into ice bath preservation in the sterile chamber; Liquid part (LAF) is separated wherein SVF.
(2) preparation of SVF: get half (about about 500ML) fatty tissue, with the centrifugal 5mim of the speed of 500G, fractionation of fatty part (PLA) and liquid part (LAF) adopt diverse ways step separation SVF wherein then respectively.
The PLA part:
1. digested 30 minutes with 37 degrees centigrade of water-baths of I-Collagen Type VI enzyme, and constantly rock.The collagenase concentration that the article of Ji Cun is used is 0.075% collagenase
2. 800g is centrifugal 10 minutes, and indigested fragment of tissue and sophisticated adipose cell can be in the upper strata of centrifuge tube, and SVF is in the bottom of centrifuge tube.
3. above bottom cell mass after centrifugal is resuspended with erythrocyte cracked liquid, room temperature effect 5min;
4. the disposable cell screen filtration with 100 μ m obtains unicellular with fragment of tissue and the impurity of removing wherein
5. use the DMEM re-suspended cell, with 300G centrifugal 5 minutes, repeat 3 times.
The LAF part:
1. 400g is centrifugal 10 minutes, and SVF is in the bottom of centrifuge tube;
2. above bottom cell mass after centrifugal is resuspended with erythrocyte cracked liquid, room temperature effect 5min;
3. the disposable cell screen filtration with 100 μ m obtains unicellular with fragment of tissue and the impurity of removing wherein
4. use the DMEM re-suspended cell, with 300G centrifugal 5 minutes, repeat 3 times.
(3) preparation of complete CAL graft: at first that amplification is good fat stem cell adds among the SVF that newly prepares, by 10
7The ratio of/100ml fat is added, at last with the fatty tissue mixing of SVF and transplanting.
Claims (5)
1. the fatty tissue of the enrichment autologous stem cells preparation methods of enlarging the bosom is characterized by, and mainly comprises the steps:
(1) Wicresoft extracts fatty tissue on one's body the sufferer, through steps such as separation and Extraction, purification, detection, amplifications, obtains the stem cell of high quantity in the GMP laboratory;
(2) general anesthesia is extracted fatty tissue with intubate and negative pressure liposuction machine;
(3) it is centrifugal to get half fatty tissue, and the fractionation of fatty inhalation (inhalatio) obtains the transplant fat tissue, and places in the sterile chamber ice bath to preserve;
(4) get second half fatty tissue centrifugalize fats portion and liquid part, separates wherein SVF with gradient centrifugation with enzyme digestion respectively then, and counting survey activity;
(5) amplification is good fat stem cell adds among the SVF that newly prepares, by 10
7The ratio of/100ml fat is added, and at last SVF and transplant fat is organized mixing.
2. the fatty tissue of the enrichment autologous stem cells according to claim 1 preparation methods of enlarging the bosom is characterized in that: described sufferer is a Body Mass Index greater than tens years old healthy population to seven teens of 15, wherein, and on one's body except the athlete of few fat.
3. the fatty tissue of the enrichment autologous stem cells according to claim 1 and 2 preparation methods of enlarging the bosom is characterized in that: in the step (1), the amount that Wicresoft extracts fatty tissue is 5ml.
4. the fatty tissue of the enrichment autologous stem cells according to claim 1 and 2 preparation methods of enlarging the bosom, it is characterized in that: in the step (3), fatty tissue is with the centrifugal 5min of the speed of 500G.
5. the fatty tissue of the enrichment autologous stem cells according to claim 1 and 2 preparation methods of enlarging the bosom, it is characterized in that: in the step (4), fatty tissue is with the centrifugal 5min of the speed of 300G.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103667185A (en) * | 2012-08-31 | 2014-03-26 | 孙勇 | New adipose-derived stem cell technology for plastic surgery and cosmetology |
| CN105358188A (en) * | 2013-06-26 | 2016-02-24 | 斯戴弗有限责任公司 | Lipofilling with ex-vivo expanded adipose tissue-derived stem cells for cosmetic breast filling or for facial filling and/or rejuvenation |
| CN109078172A (en) * | 2018-08-27 | 2018-12-25 | 白晋 | A kind of preparation and its application based on autologous tissue |
| CN109745147A (en) * | 2019-02-28 | 2019-05-14 | 浙江省肿瘤医院 | A method for fabricating an all-autologous tissue double-layered-encapsulated breast prosthesis |
| CN110038165A (en) * | 2019-04-24 | 2019-07-23 | 成都远山原力生物科技有限公司 | A kind of enrichment high activity fatty granule cell and the fat graft of fat stem cell and its preparation method and application |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2207757C (en) * | 1994-12-16 | 2005-04-05 | Children's Medical Center Corporation | Breast tissue engineering |
| CA2170753C (en) * | 1993-09-07 | 2006-11-14 | Ernst Janzen | Improved soft tissue implant |
| CN101850132A (en) * | 2009-03-31 | 2010-10-06 | 中山大学附属第一医院 | Tissue engineering breast implant and construction method thereof |
| CN101974486A (en) * | 2010-11-25 | 2011-02-16 | 王泰华 | Method for extracting mesenchymal stem cells from trace human fatty tissues and massively culturing |
| CN102058905A (en) * | 2009-11-18 | 2011-05-18 | 四川大学 | Composite fat granule and preparation method thereof |
-
2011
- 2011-07-20 CN CN2011102032107A patent/CN102258810A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2170753C (en) * | 1993-09-07 | 2006-11-14 | Ernst Janzen | Improved soft tissue implant |
| CA2207757C (en) * | 1994-12-16 | 2005-04-05 | Children's Medical Center Corporation | Breast tissue engineering |
| CN101850132A (en) * | 2009-03-31 | 2010-10-06 | 中山大学附属第一医院 | Tissue engineering breast implant and construction method thereof |
| CN102058905A (en) * | 2009-11-18 | 2011-05-18 | 四川大学 | Composite fat granule and preparation method thereof |
| CN101974486A (en) * | 2010-11-25 | 2011-02-16 | 王泰华 | Method for extracting mesenchymal stem cells from trace human fatty tissues and massively culturing |
Non-Patent Citations (1)
| Title |
|---|
| MELVIN A.SHIFFMAN: "《Autologous Fat Transfer》", 31 December 2010 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103667185A (en) * | 2012-08-31 | 2014-03-26 | 孙勇 | New adipose-derived stem cell technology for plastic surgery and cosmetology |
| CN105358188A (en) * | 2013-06-26 | 2016-02-24 | 斯戴弗有限责任公司 | Lipofilling with ex-vivo expanded adipose tissue-derived stem cells for cosmetic breast filling or for facial filling and/or rejuvenation |
| CN105358188B (en) * | 2013-06-26 | 2019-06-28 | 斯戴弗有限责任公司 | Fat implantation is carried out with the adipose tissue derived stem cells expanded in vitro to realize the filling of cosmetic breast or face filling and/or rejuvenation |
| CN109078172A (en) * | 2018-08-27 | 2018-12-25 | 白晋 | A kind of preparation and its application based on autologous tissue |
| CN109745147A (en) * | 2019-02-28 | 2019-05-14 | 浙江省肿瘤医院 | A method for fabricating an all-autologous tissue double-layered-encapsulated breast prosthesis |
| CN109745147B (en) * | 2019-02-28 | 2021-06-29 | 浙江省肿瘤医院 | A method for fabricating an all-autologous tissue double-layered-encapsulated breast prosthesis |
| CN110038165A (en) * | 2019-04-24 | 2019-07-23 | 成都远山原力生物科技有限公司 | A kind of enrichment high activity fatty granule cell and the fat graft of fat stem cell and its preparation method and application |
| CN110038165B (en) * | 2019-04-24 | 2021-09-28 | 成都远山原力生物科技有限公司 | Fat graft for enriching high-activity fat granular cells and fat stem cells and preparation method and application thereof |
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Application publication date: 20111130 |