CN102258796A - Microbial deodorant and preparation method thereof - Google Patents
Microbial deodorant and preparation method thereof Download PDFInfo
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- CN102258796A CN102258796A CN201110177886A CN201110177886A CN102258796A CN 102258796 A CN102258796 A CN 102258796A CN 201110177886 A CN201110177886 A CN 201110177886A CN 201110177886 A CN201110177886 A CN 201110177886A CN 102258796 A CN102258796 A CN 102258796A
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Abstract
The invention applies to provide a microbial deodorant which is a microbial colony composed of lactobacillus plantarum, bacillus subtilis, saccharomyces cerevisiae and rhodopseudomonas palustris, wherein counted by 108CFU (colony-forming unit)/ml as a unit, the total viable count is not less than 10, the lactobacillus plantarum count is not less than 3, the saccharomyces cerevisiae count is not less than 3, the rhodopseudomonas palustris count is not less than 2 and the bacillus subtilis count is not less than 2. The microbial deodorant applied by the invention has stronger zymolysis ability under both aerobic and anaerobic conditions, and the beneficial bacteria mutually play a role in inhibiting the growth of putrefactive bacteria and interrupting the putrefactive process, secrete organic acid while reproducing quickly, and generate antioxidation substances, thus converting odorous substances such as NH3, H2S, indoles and the like into non-odorous substances.
Description
Technical field
The present patent application relates to a kind of microbial deodorant and preparation method thereof, belongs to environmental protection technical field.
Background technology
Odorant is of a great variety, and different osmophores is arranged in the dissimilar material molecular structures, thereby different abnormal smells from the patients and threshold value are arranged.In odorant to human health damage bigger thio-alcohol, ammonia, hydrogen sulfide, dimethyl disulfide, trimethylamine, formaldehyde, styrene, chromic acid and phenols etc. are arranged.Respiratory system, blood circulation, digestive system, hormonal system and the nervous system of odor pollution main harm human body have had a strong impact on people's the mental status, have reduced quality of life.
Raising along with China's living standards of the people, malodorous discharging constantly increases the weight of for the pollution of environment, the foul smell that is produced constantly aggravates the pollution of environment, human beings'health is caused great threat, odor pollution more and more causes people's attention, therefore, the exploitation of deodorizer and a practical focus that has become research.
Deodorizer mainly is divided into physical deodorization agent, chemical deodorizing agent, microbial deodorant, plant type deodorizer and compound deodorizer etc.Its ultimate principle of microbiocidal deodorizer is to utilize microorganism that the odorant that is dissolved in the water is absorbed in microorganism in health, makes a kind of process of its degraded by the metabolic activity of microorganism.Microbial deodorization can be divided into three phases: 1, the course of dissolution of foul gas, promptly transfer to liquid phase by gas phase; 2, odor pollutant is adsorbed, absorbs by microorganism in the aqueous solution; 3, the odor pollutant that enters microbial cell is decomposed utilization as nutrient substance by Institute of Micro-biology, and pollutant are removed.
Microbiocidal deodorizer more and more is subject to people's attention owing to its advantage that has environmental protection, can reuse.But existing microbial deodorant agent producing process is comparatively complicated, and compound bacteria is difficult for obtaining, and decomposes the poor effect of junk, and these have all limited the further application of microbial deodorant.
Summary of the invention
The present patent application promptly is at the existing above-mentioned weak point of present microbial deodorant, and a kind of NEW TYPE OF COMPOSITE bacteria microorganism deodorizer is provided, and this deodorizer can efficiently decompose junk, and the technology of production fermentation is simple, and microorganism species obtains easily.
The present patent application provides a kind of compound bacteria microbial deodorant, and described deodorizer is made up of multiple beneficial microorganism species such as photosynthetic bacteria group, lactobacillus, yeast flora, bacillus cereus group, nitrifying bacteria community and denitrification floras.
Specifically, the described microbial deodorant of the present patent application is characterized in that: the microorganism species that described deodorizer is made up of Lactobacillus plantarum, bacillus subtilis, cereuisiae fermentum and swamp Rhodopseudomonas, wherein, with 10
8CFU/ml is a unit, total viable count 〉=10, Lactobacillus plantarum 〉=3, cereuisiae fermentum 〉=3, Rhodopseudomonas palustris 〉=2, bacillus subtilis 〉=2.
CFU (Colony-Forming Units) is the meaning of colony-forming units, be with the method for a certain amount of bacterium liquid after the dilution by cast or coating, allow microorganism in it is unicellular to be dispersed on the agar plate one by one, after waiting to cultivate, each living cells just forms a bacterium colony, and CFU/mL refers to the bacterial community sum that contains in every ml sample.
Described microbial deodorant is characterized in that: in the described deodorizer, and lead tolerance≤8mg/L, arsenic content≤4mg/L, fluorinated volume≤60mg/L, and must not detect Salmonella and mycete.
Described microbial deodorant is characterized in that: described total viable count culture medium is as described below:
Carnis Bovis seu Bubali cream: 10.0g glucose: 10.0g yeast extract: 3.0g
Peptone: 10.0g KH
2PO
4: 2.0g NaCl:4.0g
Agar: 20.0g distilled water: 1000ml pH value: 6.2-6.8
And sterilization is 20 minutes under 121 ℃ condition.
Described microbial deodorant is characterized in that: described Lactobacillus plantarum culture medium is as described below:
Cheese unit hydrolysate: 10.0g glucose: 20.0g yeast extract: 5.0g
Sodium acetate: 25.0g disodium citrate: 2.0g TWEEN80:1.0g
KH
2PO
43H
2O：6.0g MnSO
44H
2O：0.15g MgSO
47H
2O：0.58g
FeSO
47H
2O:0.03g agar: 15.0g distilled water: 1000ml
PH value: 5.4 and under 120 ℃ condition the sterilization 20 minutes.
Described microbial deodorant is characterized in that: described bacillus subtilis bacterium culture medium is as described below:
Carnis Bovis seu Bubali cream: 3.0g peptone: 10.0g NaCl:2.0g
Agar: 2.0g distilled water: 1000ml pH value: 6.2-7.4
And sterilization is 15-20 minute under 121 ℃ condition.
Described microbial deodorant is characterized in that: described beer yeast bacterium culture medium is as described below:
Sucrose: 50.0g (NH
4)
2SO
4: 6.0g MgSO
47H
2O:2.5g
(NH
4)
2HPO
4：2.0g K
2SO
4：4.0g CaSO
4：2.5g
Agar: 15.0-20.0g distilled water: 1000ml pH value: 6.0
And sterilization is 15 minutes under 116 ℃ condition.
Described microbial deodorant is characterized in that: described Rhodopseudomonas palustris culture medium is as described below:
NaH
2PO
4: 5.5g DL malic acid: 1.5g sodium acetate: 2.0g
NH
4Cl：1.0g MgCl
2：0.25g CaCl
2：0.05g
NaOH:2.0g growth factor solution: 1.0ml trace element solution: 1.0ml
Agar: 20.0g distilled water: 1000ml pH value: 8.0
And sterilization is 20 minutes under 121 ℃ condition.
The present patent application also provides the preparation method of described microbial deodorant, it is characterized in that: described preparation method comprises the steps, by the strain combined sorting, selects above-mentioned composition strain and according to its ratio, produces with following technological process again:
Preserve strain → slant strains → shake-flask culture → seed tank culture → seed → mixing fermentation culture → quality inspection and reserved sample observing → packing → storage or sale.
The described microbial deodorant of the present patent application all has stronger fermentation capacity of decomposition under aerobic and anaerobic condition, utilize the combined effect of these probioticss, suppress the putrefaction bacteria growth, interrupt the carrying out of decay process, in the process of breeding oneself rapidly, secrete organic acid, produce antioxidant, make NH
3, H
2Smelly source such as S, indoles material converts non-smelly source material to.
Beneficial microbe in the described microbial deodorant, can effectively suppress the corruption decomposition of putrefaction bacteria and turn to fermented type to decompose, the organic acid substance that produces can to N, S oxide degrade (decompositions) absorb and fix, thereby quantity of refuse is reduced, can obviously remove the stench of household refuse landfill sites and terminal again, eliminate the stench of sewage, obviously reduce the content of COD, NH4-N and SS, promote the purification of sewage.Simultaneously, because ethanol, lactic acid and other antibiotics that beneficial microbe produces in growth course can effectively suppress the growth of fly larvae, the metamorphosis process of upsetting abnormal insecticide, the growth and breeding that has suppressed fly adds the malodorous elimination of rubbish, and the quantity of external fly also significantly reduces.
The described microbial deodorant of the present patent application is mainly used in following field:
1. the deodorization of house refuse, press down fly: have the extremely strong malodorous effect of elimination house refuse, odor concentration reaches the requirement of the governance standard (GB14554-93 " odorant pollutant discharge standard ", CJ/J3037-1995 " household refuse landfill sites environmental monitoring technology standard ") of national regulation, and have characteristics such as equipment is simple, energy consumption is low, small investment, have and significantly press down the fly effect, press down the fly rate and reach more than 90%;
2. the deodorization of city domestic sewage and industrial wastewater purifies.Reinforcing agent as sewage disposal system, add premixing pool and regulating reservoir, can remove the stench in the sewage disposal process, reduce COD, BOD content in city domestic sewage and the industrial wastewater (high concentrated organic wastewater) significantly, purifying water body, strengthen the disposal ability and the level of sewage disposal system, realize qualified discharge, deodorization and clean-up effect are remarkable;
3. innoxious, the recycling treatment of fecal sewage.Fecal sewage is after microbial fermentation decomposes, and it is fertile and liquid fertile feces and sewage can be made into solid biologic, can eliminate stink to greatest extent, is a kind of pollution-free, organic fertilizer that fertilizer efficiency is high, meets environmental protection requirement;
4. the cesspool pool, sewer, it is obvious that the public lavatory deodorization presses down the fly effect, plays an important role to improving air quality protection health.
Points for attention:
(1) lucifuge, 5 ℃ to 40 ℃ of storage temperatures are preserved in the sealing of shady and cool place.
(2) liquid surface can shake up the back and use if any a small amount of white Mycoderma or light-yellow precipitate.
(3) deodorizer can be used with some chemicals, but can not use simultaneously with antibacterial.
The specific embodiment
Below in conjunction with concrete embodiment the described microbial deodorant of the present patent application is described; purpose is better to understand the described technology contents of the present patent application for the public; rather than to the restriction of described technology contents; in fact; with of the improvement of identical with the present invention or approximate principle to described microbial deodorant and preparation method thereof; comprise the increase and decrease and the replacement of medium component, all within the present patent application technical scheme required for protection.
Embodiment one
The described microbial deodorant of the present patent application, the microorganism species of forming by Lactobacillus plantarum, bacillus subtilis, cereuisiae fermentum and swamp Rhodopseudomonas, wherein, with 10
8CFU/ml is a unit, total viable count 〉=10, Lactobacillus plantarum 〉=3, cereuisiae fermentum 〉=3, Rhodopseudomonas palustris 〉=2, bacillus subtilis 〉=2.
In addition, in described deodorizer, lead tolerance≤8mg/L, arsenic content≤4mg/L, fluorinated volume≤60mg/L, and must not detect Salmonella and mycete.
Described deodorizer, packing specification are 25kg/ bucket or 200kg/ bucket, pH value: 3.5-4.0, and nontoxic, have no side effect, the shelf-life is 1 year, storing mode is that room temperature, the sealing of shady and cool place are preserved, and is brown liquid.
Embodiment two
1, described microbial deodorant, wherein, the total viable count culture medium is as described below:
Carnis Bovis seu Bubali cream: 10.0g glucose: 10.0g yeast extract: 3.0g
Peptone: 10.0g KH
2PO
4: 2.0g NaCl:4.0g
Agar: 20.0g distilled water: 1000ml pH value: 6.2-6.8
And sterilization is 20 minutes under 121 ℃ condition.
2, the Lactobacillus plantarum culture medium is as described below:
Cheese unit hydrolysate: 10.0g glucose: 20.0g yeast extract: 5.0g
Sodium acetate: 25.0g disodium citrate: 2.0g TWEEN80:1.0g
KH
2PO
43H
2O：6.0g MnSO
44H
2O：0.15g MgSO
47H
2O：0.58g
FeSO
47H
2O:0.03g agar: 15.0g distilled water: 1000ml
PH value: 5.4 and under 120 ℃ condition the sterilization 20 minutes.
3, the bacillus subtilis bacterium culture medium is as described below:
Carnis Bovis seu Bubali cream: 3.0g peptone: 10.0g NaCl:2.0g
Agar: 2.0g distilled water: 1000ml pH value: 6.2-7.4
And sterilization is 15-20 minute under 121 ℃ condition.
4, the beer yeast bacterium culture medium is as described below:
Sucrose: 50.0g (NH
4)
2SO
4: 6.0g MgSO
47H
2O:2.5g
(NH
4)
2HPO
4：2.0g K
2SO
4：4.0g CaSO
4：2.5g
Agar: 15.0-20.0g distilled water: 1000ml pH value: 6.0
And sterilization is 15 minutes under 116 ℃ condition.
5, the Rhodopseudomonas palustris culture medium is as described below:
NaH
2PO
4: 5.5g DL malic acid: 1.5g sodium acetate: 2.0g
NH
4Cl：1.0g MgCl
2：0.25g CaCl
2：0.05g
NaOH:2.0g growth factor solution: 1.0ml trace element solution: 1.0ml
Agar: 20.0g distilled water: 1000ml pH value: 8.0
And sterilization is 20 minutes under 121 ℃ condition.
Embodiment three
The present patent application also provides the preparation method of described microbial deodorant, comprises the steps, by the strain combined sorting, optimizes above-mentioned composition strain and proportionate relationship thereof, produces according to following technological process again:
Preserve strain → slant strains → shake-flask culture → seed tank culture → seed → mixing fermentation culture → quality inspection and reserved sample observing → packing → storage or sale.
Embodiment four
The using method of the described microbial deodorant of the present patent application is as follows:
The deodorization of house refuse, press down fly:
Spray with 10 times of microbial deodorant diluents, every square metre of exposed surface spray 0.5-0.8 kilogram diluent, the diafiltration liquid pool: every cubic metre is sprayed 0.1-0.7 kilogram diluent.
Embodiment five
City domestic sewage and trade effluent deodorization are handled:
Sewage per ton evenly adds comprehensive distributing reservoir to by 0.01 kilogram of microbial deodorant.Different its actual amount of in time adjusting of concentration according to sewage with composition.
Embodiment six
The deodorization of filter pressing workshop is handled:
Utilize spray appliance evenly to spray by 20 times of microbial deodorant diluents, 0.5 kilogram of every square metre of spray deodorant.
Embodiment seven
Feces of toilet sewage, the deodorization of poultry colony house:
With microbial deodorant dilution agent 100-200 doubly, with aerosol apparatus evenly be sprayed at each position of poultry colony house (comprise that 7 days initial stages such as ground, corner, cage tool, fecaluria groove spray once, spray 2-3 time continuously after, but treat that stink alleviates sprinkling in 10-15 days once.
The described microbial deodorant of the present patent application, can suppress the growth of Putrefying bacteria, improve organic decomposition approach, reduce ammonia and the burst size of hydrogen sulfide and the generation of Ammonia material, the biogas amount that simultaneously also reduces bacterial decomposition and produced, thus reach the deodorant purpose; And can effectively suppress the breeding of pathogenic microorganism, prevent the generation of disease.
Claims (8)
1. microbial deodorant is characterized in that: the microorganism species that described deodorizer is made up of Lactobacillus plantarum, bacillus subtilis, cereuisiae fermentum and swamp Rhodopseudomonas, wherein, with 10
8CFU/ml is a unit, total viable count 〉=10, Lactobacillus plantarum 〉=3, cereuisiae fermentum 〉=3, Rhodopseudomonas palustris 〉=2, bacillus subtilis 〉=2.
2. microbial deodorant according to claim 1 is characterized in that: in the described deodorizer, and lead tolerance≤8mg/L, arsenic content≤4mg/L, fluorinated volume≤60mg/L, and must not detect Salmonella and mycete.
3. microbial deodorant according to claim 1 is characterized in that: described total viable count culture medium is as described below:
Carnis Bovis seu Bubali cream: 10.0g glucose: 10.0g yeast extract: 3.0g
Peptone: 10.0g KH
2PO
4: 2.0g NaCl:4.0g
Agar: 20.0g distilled water: 1000ml pH value: 6.2-6.8
And sterilization is 20 minutes under 121 ℃ condition.
4. microbial deodorant according to claim 1 is characterized in that: described Lactobacillus plantarum culture medium is as described below:
Cheese unit hydrolysate: 10.0g glucose: 20.0g yeast extract: 5.0g
Sodium acetate: 25.0g disodium citrate: 2.0g TWEEN80:1.0g
KH
2PO
43H
2O：6.0g MnSO
44H
2O：0.15g MgSO
47H
2O：0.58g
FeSO
47H
2O:0.03g agar: 15.0g distilled water: 1000ml
PH value: 5.4 and under 120 ℃ condition the sterilization 20 minutes.
5. microbial deodorant according to claim 1 is characterized in that: described bacillus subtilis bacterium culture medium is as described below:
Carnis Bovis seu Bubali cream: 3.0g peptone: 10.0g NaCl:2.0g
Agar: 2.0g distilled water: 1000ml pH value: 6.2-7.4
And sterilization is 15-20 minute under 121 ℃ condition.
6. microbial deodorant according to claim 1 is characterized in that: described beer yeast bacterium culture medium is as described below:
Sucrose: 50.0g (NH
4)
2SO
4: 6.0g MgSO
47H
2O:2.5g
(NH
4)
2HPO
4：2.0g K
2SO
4：4.0g CaSO
4：2.5g
Agar: 15.0-20.0g distilled water: 1000ml pH value: 6.0
And sterilization is 15 minutes under 116 ℃ condition.
7. microbial deodorant according to claim 1 is characterized in that: described Rhodopseudomonas palustris culture medium is as described below:
NaH
2PO
4: 5.5g DL malic acid: 1.5g sodium acetate: 2.0g
NH
4Cl：1.0g MgCl
2：0.25g CaCl
2：0.05g
NaOH:2.0g growth factor solution: 1.0ml trace element solution: 1.0ml
Agar: 20.0g distilled water: 1000ml pH value: 8.0
And sterilization is 20 minutes under 121 ℃ condition.
8. the preparation method of the described microbial deodorant of claim 1 is characterized in that: described preparation method comprises the steps, by the strain combined sorting, selects above-mentioned composition strain and according to its ratio, produces with following technological process again:
Preserve strain → slant strains → shake-flask culture → seed tank culture → seed → mixing fermentation culture → quality inspection and reserved sample observing → packing → storage or sale.
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|---|---|---|---|
| CN201110177886A CN102258796A (en) | 2011-06-28 | 2011-06-28 | Microbial deodorant and preparation method thereof |
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|---|---|---|---|
| CN201110177886A CN102258796A (en) | 2011-06-28 | 2011-06-28 | Microbial deodorant and preparation method thereof |
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| CN102258796A true CN102258796A (en) | 2011-11-30 |
Family
ID=45005759
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| CN103173384A (en) * | 2013-01-30 | 2013-06-26 | 中国科学院成都生物研究所 | Lactobacillus strain and application thereof |
| CN103357056A (en) * | 2013-07-22 | 2013-10-23 | 上海绿博生物科技发展有限公司 | Healthy biological spraying agent for removing peculiar smell and preparation method thereof |
| CN103468619A (en) * | 2013-09-26 | 2013-12-25 | 青岛蔚蓝生物集团有限公司 | Microbial preparation for purifying air |
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| CN103173384A (en) * | 2013-01-30 | 2013-06-26 | 中国科学院成都生物研究所 | Lactobacillus strain and application thereof |
| CN103357056A (en) * | 2013-07-22 | 2013-10-23 | 上海绿博生物科技发展有限公司 | Healthy biological spraying agent for removing peculiar smell and preparation method thereof |
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| CN103881929A (en) * | 2014-03-13 | 2014-06-25 | 湛江德玥生物工程有限公司 | Purification compound bacterium and purification compound probiotic preparation as well as preparation method of compound probiotic preparation |
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Application publication date: 20111130 |