CN102241653A - Membrane filtration and concentration technology of gibberellin - Google Patents
Membrane filtration and concentration technology of gibberellin Download PDFInfo
- Publication number
- CN102241653A CN102241653A CN2011101908551A CN201110190855A CN102241653A CN 102241653 A CN102241653 A CN 102241653A CN 2011101908551 A CN2011101908551 A CN 2011101908551A CN 201110190855 A CN201110190855 A CN 201110190855A CN 102241653 A CN102241653 A CN 102241653A
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- CN
- China
- Prior art keywords
- membrane
- filtration
- sheet frame
- plant hormones
- gibberellins
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229930191978 Gibberellin Natural products 0.000 title claims abstract description 32
- 239000003448 gibberellin Substances 0.000 title claims abstract description 32
- 238000005516 engineering process Methods 0.000 title claims abstract description 20
- 238000005374 membrane filtration Methods 0.000 title claims abstract description 12
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 title claims abstract description 10
- 239000012528 membrane Substances 0.000 claims abstract description 51
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 30
- 238000001728 nano-filtration Methods 0.000 claims abstract description 16
- 238000001914 filtration Methods 0.000 claims abstract description 8
- 238000001223 reverse osmosis Methods 0.000 claims abstract description 7
- 239000003375 plant hormone Substances 0.000 claims description 23
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 claims description 22
- 239000007788 liquid Substances 0.000 claims description 17
- 239000012141 concentrate Substances 0.000 claims description 14
- 238000005096 rolling process Methods 0.000 claims description 14
- 239000000706 filtrate Substances 0.000 claims description 13
- 239000006210 lotion Substances 0.000 claims description 12
- 238000005406 washing Methods 0.000 claims description 12
- 239000002033 PVDF binder Substances 0.000 claims description 4
- 239000004952 Polyamide Substances 0.000 claims description 4
- 239000004698 Polyethylene Substances 0.000 claims description 4
- 229920002301 cellulose acetate Polymers 0.000 claims description 4
- 239000002131 composite material Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 229920002492 poly(sulfone) Polymers 0.000 claims description 4
- 229920002647 polyamide Polymers 0.000 claims description 4
- 229920006393 polyether sulfone Polymers 0.000 claims description 4
- -1 polyethylene Polymers 0.000 claims description 4
- 229920000573 polyethylene Polymers 0.000 claims description 4
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 6
- 238000000605 extraction Methods 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 239000005648 plant growth regulator Substances 0.000 abstract description 2
- 239000004480 active ingredient Substances 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 239000010408 film Substances 0.000 description 10
- 239000000047 product Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 3
- 239000003814 drug Substances 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 241000223218 Fusarium Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 229930004069 diterpene Natural products 0.000 description 1
- 125000000567 diterpene group Chemical group 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
Landscapes
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention provides a membrane filtration and concentration technology of gibberellin and belongs to an extraction and production method of a plant growth regulator. The technology comprises the following steps: filtering gibberellin fermentation liquor by use of a plate ultrafiltration membrane, spiral ultrafiltration membrane or tubular ultrafiltration membrane; and then concentrating by use of a tubular nanofiltration membrane or reverse osmosis membrance. By adopting the technology, the water consumption, power consumption and steam consumption can be greatly reduced and the concentration yield can be increased; and the technology can be implemented at the room temperature and the content of the main active ingredients of gibberellin can be increased in all.
Description
Technical field
The invention belongs to the extraction preparation method of a plant growth regulators, be specifically related to a kind of filtration, concentration technology of Plant hormones regulators,gibberellins.
Background technology
Plant hormones regulators,gibberellins is a class plant hormone that extensively exists, and generally synthetic in young shoot, young root and immature seed, its chemical structure belongs to diterpenes acid, is derived and is got by the Fourth Ring skeleton.The main effect of Plant hormones regulators,gibberellins is to promote cell elongation, thereby causes that cane elongation and plant increase.The Plant hormones regulators,gibberellins that uses in the production utilizes gibberella to carry out liquid fermenting and produces, and its filtration, concentration technology generally adopt the film under vacuum concentration technology, and the consumption of this method water power vapour is big, production cycle is long, cause Plant hormones regulators,gibberellins product heat-sensitive substance effective ingredient inactivation, product quality is poor, and yield is low.
Summary of the invention
The objective of the invention is at top described defective, provide a kind of and can reduce water power vapour energy consumption, shortening production cycle, reduce heat-sensitive substance effective ingredient inactivation, improve Plant hormones regulators,gibberellins membrane filtration, the concentration technology of extract yield and product quality.
The objective of the invention is to be achieved by the following technical programs.
A kind of membrane filtration of Plant hormones regulators,gibberellins, concentration technology is characterized in that may further comprise the steps successively:
Earlier with gibberellin fermented liquid through Plate Filtration, obtain sheet frame filtrate and sheet frame washing lotion, sheet frame filtrate and sheet frame washing lotion be after flat plate ultrafiltration membrane filters again, ultrafiltrated and ultrafiltration and concentration liquid, ultrafiltration and concentration liquid returns sheet frame to be continued to filter, ultrafiltrated;
2. ultrafiltrated is adopted the rolling nanofiltration membrane to carry out one-level and concentrate 3-5v/v, adopt the tubular type nanofiltration membrane to carry out secondary and concentrate 1.5-3v/v, get concentrated solution, operating temperature is a normal temperature.
The also available rolling ultra-filtration membrane of filtrate of sheet frame described in the step 1 and sheet frame washing lotion or tubular ultra-filtration membrane filter, and mould material is a kind of in polysulfone membrane, polyethylene film, poly (ether sulfone) film, cellulose acetate film, polyvinylidene fluoride film, polyamide membrane or the composite membrane.
The specification of flat plate ultrafiltration membrane described in the step 1 is 1-10 ten thousand molecular weight cut-offs.
The specification of ultra-filtration membrane of rolling described in the step 1 and tubular ultra-filtration membrane is 1-10 ten thousand molecular weight cut-offs.
Secondary described in the step 2 concentrates can adopt reverse osmosis membrane.
The specification of nanofiltration membrane of rolling described in the step 2 and tubular type nanofiltration membrane is the 100-300 molecular weight cut-off, and mould material is a kind of in polysulfone membrane, polyethylene film, poly (ether sulfone) film, cellulose acetate film, polyvinylidene fluoride film, polyamide membrane or the composite membrane.
The specification of reverse osmosis membrane described in the step 2 is the 50-100 molecular weight cut-off.
The invention has the beneficial effects as follows: the present invention adopts flat plate ultrafiltration membrane or rolling ultra-filtration membrane to carry out extraction separation, and gibberellin fermented liquid foreign protein obtains more than 99% removing, and has improved filtrate quality; The present invention adopts rolling nanofiltration membrane, tubular type nanofiltration membrane and reverse osmosis membrane to concentrate; reduce water consumption, power consumption, the steam consumption significantly; improve and concentrate yield; normal temperature running environment based on membrane module; Plant hormones regulators,gibberellins thermo-sensitivity mainly contains effective constituent GA3 and obtains safeguard protection; Plant hormones regulators,gibberellins product G A3 content is whole to be improved, and the former medicine powder color and luster whiteness grade that rises has been saved the consumption of extraction agent when the concentrated solution that obtains carries out solvent extraction again.
Embodiment
Below in conjunction with embodiment the present invention is described further.
Embodiment one.
60 tons of gibberellin fermented liquid are through Plate Filtration, obtain 50 tons of sheet frame filtrates, 18 tons of sheet frame washing lotions, sheet frame filtrate and sheet frame washing lotion after the flat plate ultrafiltration membrane of 50,000 molecular weight cut-offs filters, get 67 tons of ultrafiltrateds, 1 ton of ultrafiltration and concentration liquid again, ultrafiltration and concentration liquid returns sheet frame to be continued to filter, get a small amount of ultrafiltrated, ultrafiltrated concentrates 67 tons of ultrafiltrateds through the one-level rolling nanofiltration membrane of 200 molecular weight cut-offs, obtains 22 tons of one-level concentrated solutions, two-pass reverse osmosis film through 80 molecular weight cut-offs concentrates, obtain 6.5 tons of final concentrated solutions of Plant hormones regulators,gibberellins, the concentrated solution yield reaches 98.1%, 16 hours working hours, power consumption 5920KWH, 20 tons of water consumptions, 2 tons of steam consumptions, Plant hormones regulators,gibberellins crystalline product GA3 content 87.5%, improve 3.1% than thin film concentration traditional technology concentrated solution yield, water consumption reduces 95%, and power consumption reduces 75%, and consumption vapour reduces 99%.Increase economic benefit 11.4%; The former medicine powder GA3 of Plant hormones regulators,gibberellins improves 3.5%.
Embodiment two.
120 tons of gibberellin fermented liquid are through Plate Filtration, obtain 100 tons of sheet frame filtrates, 36 tons of sheet frame washing lotions, sheet frame filtrate and sheet frame washing lotion are again behind the rolling ultrafiltration membrance filter of 100,000 molecular weight cut-offs, get 134 tons of ultrafiltrateds, 2 tons of ultrafiltration and concentration liquid, ultrafiltration and concentration liquid returns sheet frame to be continued to filter, get a small amount of ultrafiltrated, ultrafiltrated concentrates 134 tons of ultrafiltrateds through the one-level rolling nanofiltration of 300 molecular weight cut-offs, obtain 44 tons of one-level concentrated solutions, concentrate, obtain 13 tons of final concentrated solutions of Plant hormones regulators,gibberellins through the secondary tubular type nanofiltration membrane of 100 molecular weight cut-offs.
Embodiment three.
120 tons of gibberellin fermented liquid are through Plate Filtration, obtain 100 tons of sheet frame filtrates, 36 tons of sheet frame washing lotions, sheet frame filtrate and sheet frame washing lotion are again after the tubular ultra-filtration membrane of 10,000 molecular weight cut-offs filters, get 134.2 tons of ultrafiltrateds, 1.8 tons of ultrafiltration and concentration liquid, ultrafiltration and concentration liquid returns sheet frame to be continued to filter, get a small amount of ultrafiltrated, ultrafiltrated concentrates 134.2 tons of ultrafiltrateds through the one-level rolling nanofiltration of 100 molecular weight cut-offs, obtain 43.5 tons of one-level concentrated solutions, concentrate, obtain 13.3 tons of final concentrated solutions of Plant hormones regulators,gibberellins through the secondary tubular type nanofiltration membrane of 300 molecular weight cut-offs.
Claims (7)
1. the membrane filtration of a Plant hormones regulators,gibberellins, concentration technology is characterized in that may further comprise the steps successively:
(1) earlier with gibberellin fermented liquid through Plate Filtration, obtain sheet frame filtrate and sheet frame washing lotion, sheet frame filtrate and sheet frame washing lotion be after flat plate ultrafiltration membrane filters again, ultrafiltrated and ultrafiltration and concentration liquid, ultrafiltration and concentration liquid returns sheet frame to be continued to filter, ultrafiltrated;
(2) ultrafiltrated is adopted the rolling nanofiltration membrane carry out one-level and concentrate 3-5v/v, adopt the tubular type nanofiltration membrane to carry out secondary and concentrate 1.5-3v/v, get concentrated solution.
2. the membrane filtration of a kind of Plant hormones regulators,gibberellins according to claim 1, concentration technology, it is characterized in that: the also available rolling ultra-filtration membrane of sheet frame filtrate and sheet frame washing lotion described in the step (1) or tubular ultra-filtration membrane filter, and mould material is a kind of in polysulfone membrane, polyethylene film, poly (ether sulfone) film, cellulose acetate film, polyvinylidene fluoride film, polyamide membrane or the composite membrane.
3. the membrane filtration of a kind of Plant hormones regulators,gibberellins according to claim 1, concentration technology, it is characterized in that: the specification of flat plate ultrafiltration membrane described in the step (1) is 1-10 ten thousand molecular weight cut-offs.
4. the membrane filtration of a kind of Plant hormones regulators,gibberellins according to claim 2, concentration technology, it is characterized in that: the specification of rolling ultra-filtration membrane and tubular ultra-filtration membrane described in the step (1) is 1-10 ten thousand molecular weight cut-offs.
5. the membrane filtration of a kind of Plant hormones regulators,gibberellins according to claim 1, concentration technology is characterized in that: secondary described in the step (2) concentrates can adopt reverse osmosis membrane.
6. the membrane filtration of a kind of Plant hormones regulators,gibberellins according to claim 1, concentration technology, it is characterized in that: the specification of rolling nanofiltration membrane and tubular type nanofiltration membrane is the 100-300 molecular weight cut-off described in the step (2), and mould material is a kind of in polysulfone membrane, polyethylene film, poly (ether sulfone) film, cellulose acetate film, polyvinylidene fluoride film, polyamide membrane or the composite membrane.
7. the membrane filtration of a kind of Plant hormones regulators,gibberellins according to claim 5, concentration technology, it is characterized in that: the specification of reverse osmosis membrane is the 50-100 molecular weight cut-off described in the step (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110190855 CN102241653B (en) | 2011-07-08 | 2011-07-08 | Membrane filtration and concentration technology of gibberellin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110190855 CN102241653B (en) | 2011-07-08 | 2011-07-08 | Membrane filtration and concentration technology of gibberellin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102241653A true CN102241653A (en) | 2011-11-16 |
| CN102241653B CN102241653B (en) | 2013-09-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110190855 Active CN102241653B (en) | 2011-07-08 | 2011-07-08 | Membrane filtration and concentration technology of gibberellin |
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| Country | Link |
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| CN (1) | CN102241653B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102775372A (en) * | 2012-07-19 | 2012-11-14 | 浙江钱江生物化学股份有限公司 | Application method of reverse osmose membrane technology in gibberellin extraction |
| CN103360355A (en) * | 2012-12-28 | 2013-10-23 | 三达膜科技（厦门）有限公司 | Method for extracting gibberellin from fermentation liquor |
| CN103641868A (en) * | 2013-11-18 | 2014-03-19 | 宁夏泰瑞制药股份有限公司 | Method for producing daunorubicin hydrochloride by utilizing daunorubicin fermentation broth |
| CN105541603A (en) * | 2015-12-23 | 2016-05-04 | 江西新瑞丰生化有限公司 | Method for separating natural abscisic acid crude product from abscisic acid fermentation broth |
| CN105622559A (en) * | 2016-02-03 | 2016-06-01 | 昆山东大智汇技术咨询有限公司 | Gibberellin fermentation liquid membrane extraction water crystallization process |
| CN106749140A (en) * | 2017-01-23 | 2017-05-31 | 同舟纵横（厦门）流体技术有限公司 | A kind of gibberellin high power concentration technology and concentration systems |
| CN108176226A (en) * | 2018-01-18 | 2018-06-19 | 四川龙蟒福生科技有限责任公司 | The membrane filtration method for concentration of gibberellic acid and membrane filtration concentration systems |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1055738A (en) * | 1990-04-16 | 1991-10-30 | 中外合资福州榕山生化制品开发有限公司 | Gibberellin extraction process |
-
2011
- 2011-07-08 CN CN 201110190855 patent/CN102241653B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1055738A (en) * | 1990-04-16 | 1991-10-30 | 中外合资福州榕山生化制品开发有限公司 | Gibberellin extraction process |
Non-Patent Citations (1)
| Title |
|---|
| 韦海宁: "赤霉素提取方案的研究", 《上海化工》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102775372A (en) * | 2012-07-19 | 2012-11-14 | 浙江钱江生物化学股份有限公司 | Application method of reverse osmose membrane technology in gibberellin extraction |
| CN103360355A (en) * | 2012-12-28 | 2013-10-23 | 三达膜科技（厦门）有限公司 | Method for extracting gibberellin from fermentation liquor |
| CN103360355B (en) * | 2012-12-28 | 2015-10-21 | 三达膜科技（厦门）有限公司 | The method of Plant hormones regulators,gibberellins is extracted from fermented liquid |
| CN103641868A (en) * | 2013-11-18 | 2014-03-19 | 宁夏泰瑞制药股份有限公司 | Method for producing daunorubicin hydrochloride by utilizing daunorubicin fermentation broth |
| CN103641868B (en) * | 2013-11-18 | 2015-12-09 | 宁夏泰瑞制药股份有限公司 | A kind of method utilizing daunorubicin fermentation liquor to produce daunorubicin hydrochloride |
| CN105541603A (en) * | 2015-12-23 | 2016-05-04 | 江西新瑞丰生化有限公司 | Method for separating natural abscisic acid crude product from abscisic acid fermentation broth |
| CN105541603B (en) * | 2015-12-23 | 2019-04-19 | 江西新瑞丰生化有限公司 | A method of separating natural falls off acid crude from abscisic acid fermentation liquid |
| CN105622559A (en) * | 2016-02-03 | 2016-06-01 | 昆山东大智汇技术咨询有限公司 | Gibberellin fermentation liquid membrane extraction water crystallization process |
| CN106749140A (en) * | 2017-01-23 | 2017-05-31 | 同舟纵横（厦门）流体技术有限公司 | A kind of gibberellin high power concentration technology and concentration systems |
| CN108176226A (en) * | 2018-01-18 | 2018-06-19 | 四川龙蟒福生科技有限责任公司 | The membrane filtration method for concentration of gibberellic acid and membrane filtration concentration systems |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102241653B (en) | 2013-09-25 |
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Address after: Ji'an City Chengnan Industrial Zone 331300 Jiangxi Xingan Patentee after: Jiangxi new Ruifeng biochemical Limited by Share Ltd Address before: Ji'an City Chengnan Industrial Zone 331300 Jiangxi Xingan Patentee before: Jiangxi Xinruifeng Biochemical Co., Ltd. |
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