CN102205142B - Water-soluble hemostatic material and preparation method thereof - Google Patents

Abstract

The invention provides a water-soluble hemostatic material and a preparation method thereof and relates to a hemostatic material and a preparation method thereof, wherein the water-soluble hemostatic material and the preparation method thereof can be used for overcoming the problems that the existing oxidized regenerated cellulose materials are not water-soluble, have poor biological absorbability and slow hemostatic speed. The water-soluble hemostatic material is prepared from oxidized regenerated cellulose carboxylic sodium fabrics and hydrochloric acid. The preparation method comprises the following steps: winding the oxidized regenerated cellulose carboxylic sodium fabrics on a glass porous core of an acidification rotary reactor; adding the hydrochloric acid for close acidification; decompressing, filtering and washing with absolute ethanol; and freezing and drying to obtain the water-soluble hemostatic material. The water-soluble hemostatic material can be used as a hemostatic material.

Description

Water-soluble hemostatic material and preparation method thereof

technical field

The invention relates to a hemostatic material and a preparation method thereof.

Background technique

Oxidized regenerated cellulose (ORC) has been well known and widely used as a hemostatic material in the medical field. Oxidized regenerated cellulose is made of regenerated cellulose braided fabric, and it is selectively oxidized with a suitable oxidation system (such as nitrogen dioxide or TEMPO system) to obtain a carboxyl content between 3.56 and 5.33mmol/g. The oxidized regenerated cellulose fabric is then washed, dried, packaged and sterilized to make clinical medical oxidized regenerated cellulose hemostatic products.

Oxidized regenerated cellulose can activate platelets in the wound and accelerate hemostasis. Moreover, as certain components in the blood are adsorbed to the surface of the oxidized regenerated cellulose, the oxidized regenerated cellulose will slowly expand, and then compress the broken end of the blood vessel to stop the bleeding, but it is only suitable for small wounds, low bleeding volume, and the hemostasis speed is slow .

Since oxidized regenerated cellulose is a water-insoluble hemostatic material, its pH value is about 3.5, and it can only be dissolved in alkaline solutions with a pH value in the range of 10-14. Therefore, the bioabsorbability of oxidized regenerated cellulose in the body is poor, and the acidity of oxidized regenerated cellulose is strong, which may cause damage to some nervous systems, thereby limiting the application of oxidized regenerated cellulose in sensitive parts such as the brain . At the same time, the acidity of the oxidized regenerated cellulose hemostatic material will also cause some acid-sensitive drugs to fail, such as thrombin.

The acidic carboxyl structure in oxidized regenerated cellulose can combine with Fe ³⁺ in hemoglobin to play a certain hemostatic effect, but in the process of clinical use, the carboxyl group in oxidized regenerated cellulose will be quickly neutralized by buffer substances in the blood , so this hemostatic effect is short-lived and insignificant.

Research on the modification of oxidized regenerated cellulose as a hemostatic material has been in continuous effort. Doub et al. disclose a method of neutralizing oxidized regenerated cellulose with an aqueous solution of sodium bicarbonate or calcium acetate, impregnating the neutralized oxidized regenerated cellulose with thrombin, and then freezing the impregnated fabric And drying in this state, a highly efficient oxidized cellulose surgical hemostatic material is prepared. In the U.S. patent, Saferstein et al. describe the use of an alcoholic aqueous solution of a weak acid salt, such as sodium acetate, to neutralize the oxidized regenerated cellulose to a pH value between 5 and 8. This method not only makes the oxidized regenerated cellulose stable at room temperature storage, and can be loaded with acid-sensitive substances like thrombin, thereby improving the hemostatic performance of oxidized regenerated cellulose materials. At the same time, they also found that the method of neutralization by Doub et al. with sodium bicarbonate would lead to partial gelation and deformation of the oxidized regenerated cellulose fabric, and the tensile strength of the final neutralized oxidized regenerated cellulose fabric would be too low to be applied in practice. in the process of hemostasis. Although the oxidized regenerated cellulose neutralized by calcium acetate mentioned in the patent by Doub et al. has guaranteed the original shape of the fabric, due to the high calcium content of the material after neutralization, it will be harmful to mammals at the contact point during use. Skin and other body cells are irritated and form large whitish granulation bumps at the site of application, preventing bioabsorption of the material. The above method can improve the hemostatic performance of oxidized regenerated cellulose by modification, but its bioabsorbability is greatly reduced. In another U.S. patent, Stilwell et al. prepared a calcium-modified oxidized regenerated cellulose hemostatic material with a neutralization method. They found that when the calcium content in oxidized cellulose was between 0.4 and 5.0 Over time, the hemostatic properties of calcium-modified oxidized cellulose were superior to those of unmodified oxidized regenerated cellulose and sodium or potassium-modified oxidized regenerated cellulose, and the effect on the bioabsorbability of the material was not too great. When calcium and sodium or potassium are mixed and modified, the obtained modified oxidized regenerated cellulose has a higher pH value to meet the conditions of compatibility with acid-sensitive substances, and the calcium contained therein can achieve enhanced hemostatic effect, It will not cause biological tissue irritation.

Since oxidized regenerated cellulose can be completely dissolved in an alkaline solution with a pH value of 10 to 14, and the strength is greatly reduced or even the shape of the oxidized regenerated cellulose fabric cannot be maintained. Therefore, weak acid salt systems have been used in previous patents and researches. Neutralizes modified oxidized cellulose. Although the neutralization and modification of the oxidized regenerated cellulose hemostatic material in the above research weakens the acidity of the oxidized regenerated cellulose hemostatic material, the products after neutralizing the oxidized regenerated cellulose with weak acid salts are all water-insoluble, polymeric The degree of reduction in the degree of reduction is very small, or even does not degrade, so there are still defects in the poor bioabsorbability and the inability to improve its hemostasis speed.

Contents of the invention

The present invention aims to solve the problems that the existing oxidized regenerated cellulose materials are non-water-soluble, have poor bioabsorbability and slow hemostasis speed, and prepare a new oxidized regenerated cellulose from the perspective of neutralization and modification different from the existing oxidized regenerated cellulose. A water-soluble oxidized regenerated cellulose hemostatic material with a gradient distribution of functional group concentrations.

The water-soluble hemostatic material is made of oxidized regenerated cellulose sodium carboxylate fabric and hydrochloric acid, the carboxyl functional group content in the water-soluble hemostatic material is 0.10-5.21mmol/g, and the acidification degree of sodium carboxylate in the oxidized regenerated cellulose sodium carboxylate fabric is 3%～98%; Among them, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.30～4.77mmol/g, the molar ratio of sodium carboxylate in the oxidized regenerated cellulose sodium carboxylate fabric to hydrogen ions in hydrochloric acid It is (100:1) ~ (1:20).

The above-mentioned water-soluble hemostatic material is prepared according to the following steps:

Wrap the oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core of the acidification rotary reactor, then add hydrochloric acid for closed acidification for 0.5-72 hours, the acidification temperature is 10-30°C, and then rinse with absolute ethanol for 3-3~ 5 times, and then freeze-dried in an environment of -10 to -80°C for 24 to 120 hours to obtain a water-soluble hemostatic material;

Wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.30～4.77mmol/g, and the molar ratio of sodium carboxylate and hydrogen ion in the hydrochloric acid in the oxidized regenerated cellulose sodium carboxylate fabric is (100:1) ~(1:20); the rotating speed of the acidification rotary reactor is 100～400r/min, and the hydrochloric acid added to the acidification rotary reactor does not directly contact with the oxidized regenerated cellulose sodium carboxylate fabric when the acidification rotary reactor does not start .

In the present invention, hydrochloric acid is used to controlly acidify the oxidized regenerated cellulose sodium carboxylate fabric, and the sodium carboxylate structure part in the oxidized regenerated cellulose sodium carboxylate fabric is converted into a carboxylic acid structure, because the oxidized regenerated cellulose sodium carboxylate used There is no carboxyl group in the fabric structure (the carboxyl functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 0), so the acidified carboxylic acid functional group concentration presents a gradient distribution during the acidification process, and the original shape can still be maintained; due to the addition of The hydrochloric acid in the acidification rotary reactor does not contact the oxidized regenerated cellulose sodium carboxylate fabric when the acidification rotary reactor is not started, so the oxidized regenerated cellulose sodium carboxylate fabric does not dissolve, and its strength meets the clinical hemostasis requirements.

In addition to the carboxylic acid functional groups formed by acidification, the water-soluble hemostatic material of the present invention also has some sodium carboxylate functional groups. Therefore, the acidity of the water-soluble hemostatic material is weaker than that of the oxidized regenerated cellulose material, which can reduce or even eliminate the occurrence of nerve damage in the human body. The possibility of system damage, thereby expanding the application range of hemostatic materials, and the weak acidity of the water-soluble hemostatic materials of the present invention can be used in combination with some acid-sensitive hemostatic materials or drugs, which is expected to further produce faster Hemostatic products.

The concentration of carboxylic acid functional groups in the water-soluble hemostatic material of the present invention presents a gradient distribution, the content of sodium carboxylate functional groups gradually increases from the surface to the inside, and the content of carboxylic acid functional groups gradually decreases from the surface to the inside. The functional group concentration gradient distribution of the water-soluble hemostatic material of the present invention can produce different hemostatic mechanisms in different periods of hemostasis, which is more suitable for the bleeding process of the wound; the water-soluble hemostatic material of the present invention is in contact with blood at the initial stage, and the amount of blood absorbed by it is relatively small. The hemostatic effect mainly depends on the combination of carboxylic acid functional groups distributed on the surface and Fe ³⁺ in hemoglobin. With the increase of the amount of blood absorbed by the water-soluble hemostatic material, the blood penetrates into the water-soluble hemostatic material, and the carboxylic acid in the water-soluble hemostatic material The sodium functional group is water-soluble, and under the action of the sodium carboxylate functional group, the water-soluble hemostatic material quickly forms a gel, which fills the wound surface gap, oppresses and blocks the end of the blood vessel to stop bleeding. The water-soluble hemostatic material of the present invention can degrade rapidly to form a gel in a short time after encountering water or saline solution. Therefore, the dual hemostatic mechanisms of the water-soluble hemostatic material of the present invention, both biological hemostasis and physical hemostasis, play a role in a short time, and Greatly shorten the hemostatic time and improve the hemostatic effect. Moreover, the water-soluble hemostatic material of the present invention can also activate platelets at the wound to accelerate hemostasis.

Since the sodium carboxylate functional group in the water-soluble hemostatic material of the present invention has water solubility, the water-soluble hemostatic material of the present invention has better water solubility and degradability than oxidized regenerated cellulose, and can be absorbed by metabolism faster in the living body .

The water-soluble hemostatic material of the invention does not decompose or degrade during the preparation process, and the preparation method is simple, and the reaction conditions are easy to operate and control. In the preparation process of the water-soluble hemostatic material of the present invention, the absolute ethanol is only used for washing, and the dosage is small, which can save a lot of cost.

Description of drawings

Fig. 1 is a structural schematic diagram of an acidification rotary reactor, and the rotation direction is the direction of the arrow in Fig. 1 . Figure 2 is a sectional view of an acidification rotary reactor. Figure 3 is a cross-section of an acidification rotary reactor. Fig. 4 is an observation diagram after placing the oxidized regenerated cellulose hemostatic material on the wound surface for 60 seconds. Fig. 5 is an observation diagram after placing the water-soluble hemostatic material prepared in Embodiment 28 above the wound for 60 seconds.

Detailed ways

The technical solution of the present invention is not limited to the specific embodiments listed below, but also includes any combination of the specific embodiments.

Specific Embodiment 1: In this embodiment, the water-soluble hemostatic material is made of oxidized regenerated cellulose sodium carboxylate fabric and hydrochloric acid. The carboxyl functional group content in the water-soluble hemostatic material is 0.10-5.2 mmol/g. The acidification degree of sodium carboxylate is 3% to 98%; wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.30 to 4.77mmol/g, and the sodium carboxylate in the oxidized regenerated cellulose sodium carboxylate fabric The molar ratio of hydrogen ion to hydrochloric acid is (100:1)~(1:20).

In this embodiment, the carboxyl functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is zero.

Oxidized regenerated cellulose sodium carboxylate fabric is made by oxidation system of 2,2,6,6-tetramethylpiperidine oxide free radical TEMPO-NaBr-NaClO and regenerated cellulose fabric, wherein TEMPO(2, The quality of 2,6,6-tetramethylpiperidine oxide) is 0.1%～8.0% of the quality of regenerated cellulose fabric, the quality of NaBr is 5.0%～80.0% of the quality of regenerated cellulose fabric, the available chlorine of NaClO solution The content is 5%-16%, the volume of NaClO solution is 30-80ml, and the NaClO solution is added in batches, 5-10ml each time; the oxidation reaction temperature is 0-15°C, and the pH value of the oxidation system is between 9.0-11.0 , the stirring speed is 200-400 rev/min. Specific examples of the preparation method of oxidized regenerated cellulose sodium carboxylate fabric: 2g floc viscose fabric, TEMPO 100mg, NaBr 0.96g, NaClO solution 60ml, react at 8°C, and keep the concentration of the reaction solution with 0.4mol/L NaOH solution When the pH is around 10.0, add NaClO solution in 4 times. When 31ml NaOH is consumed, the reaction is terminated, and the reaction solution is poured into excess ethanol (300ml), and the precipitate is oxidized regenerated cellulose sodium carboxylate fabric.

Embodiment 2: The difference between this embodiment and Embodiment 1 is that the concentration of hydrochloric acid is 0.01-2.00 mol/L. Others are the same as the first embodiment.

Embodiment 3: The difference between this embodiment and Embodiment 1 is that the concentration of hydrochloric acid is 0.10-1.50 mol/L. Others are the same as the first embodiment.

Embodiment 4: The difference between this embodiment and Embodiments 1 to 3 is that the content of carboxyl functional groups in the water-soluble hemostatic material is 0.17-2.25 mmol/g, and the content of sodium carboxylate in the oxidized regenerated cellulose sodium carboxylate fabric is The degree of acidification is 5% to 45%. Others are the same as those of Embodiments 1 to 3.

Embodiment 5: The difference between this embodiment and Embodiment 1 to Embodiment 5 is that the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.40-4.14 mmol/g. Others are the same as one of Embodiments 1 to 4.

Specific embodiment six: the difference between this embodiment and one of the specific embodiments one to five is: the mol ratio of sodium carboxylate and hydrogen ion in hydrochloric acid in the oxidized regenerated cellulose sodium carboxylate fabric is (4: 1)～(1 : 10). Others are the same as one of Embodiments 1 to 4.

Embodiment 7: In this embodiment, the water-soluble hemostatic material is prepared according to the following steps:

Wrap the oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core of the acidification rotary reactor, then add hydrochloric acid for closed acidification for 0.5-72 hours, the acidification temperature is 10-30°C, and then rinse with absolute ethanol for 3-3~ 5 times, and then freeze-dried in an environment of -10 to -80°C for 24 to 120 hours to obtain a water-soluble hemostatic material;

Wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.30～4.77mmol/g, and the molar ratio of sodium carboxylate and hydrogen ion in the hydrochloric acid in the oxidized regenerated cellulose sodium carboxylate fabric is (100:1) ~(1:20); the rotating speed of the acidification rotary reactor is 100～400r/min, and the hydrochloric acid added to the acidification rotary reactor does not directly contact with the oxidized regenerated cellulose sodium carboxylate fabric when the acidification rotary reactor does not start .

Oxidized regenerated cellulose sodium carboxylate fabric is made by oxidation system of 2,2,6,6-tetramethylpiperidine oxide free radical TEMPO-NaBr-NaClO and regenerated cellulose fabric, wherein TEMPO(2, The quality of 2,6,6-tetramethylpiperidine oxide) is 0.1%～8.0% of the quality of regenerated cellulose fabric, the quality of NaBr is 5.0%～80.0% of the quality of regenerated cellulose fabric, the available chlorine of NaClO solution The content is 5%-16%, the volume of NaClO solution is 30-80ml, and the NaClO solution is added in batches, 5-10ml each time; the oxidation reaction temperature is 0-15°C, and the pH value of the oxidation system is between 9.0-11.0 , the stirring speed is 200-400 rev/min. Specific examples of the preparation method of oxidized regenerated cellulose sodium carboxylate fabric: 2g floc viscose fabric, TEMPO 100mg, NaBr 0.96g, NaClO solution 60ml, react at 8°C, and keep the concentration of the reaction solution with 0.4mol/L NaOH solution When the pH is around 10.0, add NaClO solution in 4 times. When 31ml NaOH is consumed, the reaction is terminated, and the reaction solution is poured into excess ethanol (300ml), and the precipitate is oxidized regenerated cellulose sodium carboxylate fabric.

The content of carboxyl functional groups in the water-soluble hemostatic material of this embodiment is 0.10-5.21 mmol/g, and the acidification degree of sodium carboxylate in the oxidized regenerated cellulose sodium carboxylate fabric is 3%-98%.

The schematic diagram of the structure of the acidification rotary reactor is shown in Figure 1, the cross section of the acidification rotary reactor is shown in Figure 2, the cross section of the acidification rotary reactor is shown in Figure 3, and the glass porous core 1 is located on the axis of the acidification rotary reactor. core, the direction of rotation is shown in Figure 1.

In the acidification process of this embodiment, the hydrochloric acid is brought to the top of the acidification rotary reactor by the raised ribs 2 inside the acidification rotary reactor, and then falls by gravity to the oxidized regenerated cellulose carboxylate wound on the glass porous core 1 of the acidification rotary reactor. Sodium acid fabric contact.

Embodiment 8: The difference between this embodiment and Embodiment 7 is that the concentration of hydrochloric acid is 0.01-2.00 mol/L. Other steps and parameters are the same as those in Embodiment 7.

Embodiment 9: The difference between this embodiment and Embodiment 7 is that the concentration of hydrochloric acid is 0.10-1.50 mol/L. Other steps and parameters are the same as those in Embodiment 7.

Embodiment 10: The difference between this embodiment and Embodiment 7 to Embodiment 9 is that the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.40-4.14 mmol/g. Other steps and parameters are the same as those in the seventh to ninth embodiments.

Embodiment eleven: the difference between this embodiment and embodiment seven to ten is: the mol ratio of sodium carboxylate and hydrogen ion in hydrochloric acid in the oxidized regenerated cellulose sodium carboxylate fabric is (4: 1)～ (1:10). Other steps and parameters are the same as Embodiments 7 to 11.

Embodiment 12: This embodiment differs from Embodiment 7 to Embodiment 11 in that: the acidification time is 8-48 hours, and the acidification temperature is 20-25°C. Other steps and parameters are the same as those in Embodiments 7 to 11.

This embodiment can adjust the acidification degree of the water-soluble hemostatic material, and endow the water-soluble hemostatic material with suitable strength and hemostatic effect.

Specific Embodiment Thirteen: The difference between this embodiment and Embodiments VII to Twelve is that the freeze-drying temperature is -20-65° C., and the freeze-drying time is 48-72 hours. Other steps and parameters are the same as those of Embodiments 7 to 12.

Specific Embodiment Fourteen: In this embodiment, the water-soluble hemostatic material is prepared according to the following steps:

Wrap 5.00g of oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor, then add 55.77ml of hydrochloric acid with a concentration of 0.01mol/L, and conduct airtight acidification for 24 hours at a temperature of 25°C, then use Water-soluble hemostatic material was obtained by vacuum filtration and washing with absolute ethanol for 5 times, and then freeze-drying at -50°C for 48 hours;

Wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.38mmol/g; Do not directly contact the oxidized regenerated cellulose sodium carboxylate fabric.

The schematic diagram of the structure of the acidification rotary reactor is shown in Figure 1, the cross section of the acidification rotary reactor is shown in Figure 2, the cross section of the acidification rotary reactor is shown in Figure 3, and the glass porous core 1 is located on the axis of the acidification rotary reactor. core, the direction of rotation is shown in Figure 1.

During the acidification process, the hydrochloric acid is carried from the raised corrugation 2 inside the acidification rotary reactor to the top inside the acidification rotary reactor, and then falls by gravity and is wound on the oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor touch.

Titration of the carboxyl functional group content of the water-soluble hemostatic material in this embodiment is 0.11 mmol/g, indicating that the acidification degree is 3.10%.

In this embodiment, the water-soluble hemostatic material can quickly form a gel within 10 seconds after encountering water or saline solution.

Embodiment 15: In this embodiment, the water-soluble hemostatic material is prepared according to the following steps:

Wrap 5.00 g of oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor, then add 135.20 ml of hydrochloric acid with a concentration of 0.01 mol/L, and carry out airtight acidification for 24 hours at a temperature of 25 °C, and then use Water-soluble hemostatic material was obtained by vacuum filtration and washing with absolute ethanol for 5 times, and then freeze-drying at -50°C for 48 hours;

Wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.38mmol/g; Do not directly contact the oxidized regenerated cellulose sodium carboxylate fabric.

The schematic diagram of the structure of the acidification rotary reactor is shown in Figure 1, the cross section of the acidification rotary reactor is shown in Figure 2, the cross section of the acidification rotary reactor is shown in Figure 3, and the glass porous core 1 is located on the axis of the acidification rotary reactor. core, the direction of rotation is shown in Figure 1.

During the acidification process, the hydrochloric acid is carried from the raised corrugation 2 inside the acidification rotary reactor to the top inside the acidification rotary reactor, and then falls by gravity and is wound on the oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor touch.

Titration of the carboxyl functional group content of the water-soluble hemostatic material in this embodiment is 0.27 mmol/g, indicating that the acidification degree is 7.93%.

In this embodiment, the water-soluble hemostatic material can quickly form a gel within 10 seconds after encountering water or saline solution.

Specific Embodiment Sixteen: In this embodiment, the water-soluble hemostatic material is prepared according to the following steps:

Wrap 5.00g of oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor, then add 1690.00ml of hydrochloric acid with a concentration of 0.01mol/L, and conduct airtight acidification for 24 hours at a temperature of 25°C. Water-soluble hemostatic material was obtained by vacuum filtration and washing with absolute ethanol for 5 times, and then freeze-drying at -50°C for 48 hours;

Wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.38mmol/g; Do not directly contact the oxidized regenerated cellulose sodium carboxylate fabric.

The schematic diagram of the structure of the acidification rotary reactor is shown in Figure 1, the cross section of the acidification rotary reactor is shown in Figure 2, the cross section of the acidification rotary reactor is shown in Figure 3, and the glass porous core 1 is located on the axis of the acidification rotary reactor. core, the direction of rotation is shown in Figure 1.

During the acidification process, the hydrochloric acid is carried from the raised corrugation 2 inside the acidification rotary reactor to the top inside the acidification rotary reactor, and then falls by gravity and is wound on the oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor touch.

Titration of the carboxyl functional group content of the water-soluble hemostatic material in this embodiment is 3.51 mmol/g, indicating that the acidification degree is 96.37%.

In this embodiment, the water-soluble hemostatic material can rapidly form a gel within 120 seconds after encountering water or saline solution.

Specific Embodiment Seventeen: In this embodiment, the water-soluble hemostatic material is prepared according to the following steps:

Wrap 5.00 g of oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor, then add 11.83 ml of hydrochloric acid with a concentration of 0.50 mol/L, and conduct airtight acidification for 48 hours at a temperature of 25 °C, and then use Water-soluble hemostatic material was obtained by vacuum filtration and washing with absolute ethanol for 5 times, and then freeze-dried at -50°C for 72 hours;

Wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.38mmol/g; Do not directly contact the oxidized regenerated cellulose sodium carboxylate fabric.

The schematic diagram of the structure of the acidification rotary reactor is shown in Figure 1, the cross section of the acidification rotary reactor is shown in Figure 2, the cross section of the acidification rotary reactor is shown in Figure 3, and the glass porous core 1 is located on the axis of the acidification rotary reactor. core, the direction of rotation is shown in Figure 1.

During the acidification process, the hydrochloric acid is carried from the raised corrugation 2 inside the acidification rotary reactor to the top inside the acidification rotary reactor, and then falls by gravity and is wound on the oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor touch.

Titration of the carboxyl functional group content of the water-soluble hemostatic material in this embodiment is 1.19 mmol/g, indicating that the acidification degree is 34.22%.

In this embodiment, the water-soluble hemostatic material can quickly form a gel within 60 seconds after encountering water or saline solution.

Embodiment 18: In this embodiment, the water-soluble hemostatic material is prepared according to the following steps:

Wrap 5.00 g of oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor, then add 6.65 ml of hydrochloric acid with a concentration of 0.20 mol/L, and conduct airtight acidification for 48 hours at a temperature of 25 °C, and then use Water-soluble hemostatic material was obtained by vacuum filtration and washing with absolute ethanol for 5 times, and then freeze-dried at -50°C for 72 hours;

Wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.80mmol/g; Do not directly contact the oxidized regenerated cellulose sodium carboxylate fabric.

The schematic diagram of the structure of the acidification rotary reactor is shown in Figure 1, the cross section of the acidification rotary reactor is shown in Figure 2, the cross section of the acidification rotary reactor is shown in Figure 3, and the glass porous core 1 is located on the axis of the acidification rotary reactor. core, the direction of rotation is shown in Figure 1.

During the acidification process, the hydrochloric acid is carried from the raised corrugation 2 inside the acidification rotary reactor to the top inside the acidification rotary reactor, and then falls by gravity and is wound on the oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor touch.

Titration of the carboxyl functional group content of the water-soluble hemostatic material in this embodiment is 0.25 mmol/g, indicating that the acidification degree is 6.64%.

In this embodiment, the water-soluble hemostatic material can quickly form a gel within 10 seconds after encountering water or saline solution.

Specific Embodiment Nineteen: In this embodiment, the water-soluble hemostatic material is prepared according to the following steps:

Wrap 5.00 g of oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor, then add 14.25 ml of hydrochloric acid with a concentration of 0.20 mol/L, and conduct airtight acidification for 48 hours at a temperature of 25 °C, and then use Water-soluble hemostatic material was obtained by vacuum filtration and washing with absolute ethanol for 5 times, and then freeze-dried at -50°C for 72 hours;

Wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.80mmol/g; Do not directly contact the oxidized regenerated cellulose sodium carboxylate fabric.

The schematic diagram of the structure of the acidification rotary reactor is shown in Figure 1, the cross section of the acidification rotary reactor is shown in Figure 2, the cross section of the acidification rotary reactor is shown in Figure 3, and the glass porous core 1 is located on the axis of the acidification rotary reactor. core, the direction of rotation is shown in Figure 1.

During the acidification process, the hydrochloric acid is carried from the raised corrugation 2 inside the acidification rotary reactor to the top inside the acidification rotary reactor, and then falls by gravity and is wound on the oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor touch.

Titration of the carboxyl functional group content of the water-soluble hemostatic material in this embodiment is 0.56 mmol/g, indicating that the acidification degree is 14.50%.

In this embodiment, the water-soluble hemostatic material can quickly form a gel within 30 seconds after encountering water or saline solution.

Specific Embodiment Twenty: In this embodiment, the water-soluble hemostatic material is prepared according to the following steps:

Wrap 5.00 g of oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor, then add 28.5 ml of hydrochloric acid with a concentration of 0.20 mol/L, and conduct airtight acidification for 48 hours at a temperature of 25 °C, and then use Water-soluble hemostatic material was obtained by vacuum filtration and washing with absolute ethanol for 5 times, and then freeze-dried at -50°C for 72 hours;

Wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.80mmol/g; Do not directly contact the oxidized regenerated cellulose sodium carboxylate fabric.

The schematic diagram of the structure of the acidification rotary reactor is shown in Figure 1, the cross section of the acidification rotary reactor is shown in Figure 2, the cross section of the acidification rotary reactor is shown in Figure 3, and the glass porous core 1 is located on the axis of the acidification rotary reactor. core, the direction of rotation is shown in Figure 1.

During the acidification process, the hydrochloric acid is carried from the raised corrugation 2 inside the acidification rotary reactor to the top inside the acidification rotary reactor, and then falls by gravity and is wound on the oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor touch.

Titration of the carboxyl functional group content of the water-soluble hemostatic material in this embodiment is 1.12 mmol/g, indicating that the acidification degree is 28.87%.

In this embodiment, the water-soluble hemostatic material can quickly form a gel within 60 seconds after encountering water or saline solution.

Specific Embodiment Twenty-one: In this embodiment, the water-soluble hemostatic material is prepared according to the following steps:

Wrap 5.00 g of oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor, then add 38.00 ml of hydrochloric acid with a concentration of 0.20 mol/L, and conduct airtight acidification for 48 hours at a temperature of 25 °C, and then use Water-soluble hemostatic material was obtained by vacuum filtration and washing with absolute ethanol for 5 times, and then freeze-dried at -50°C for 72 hours;

Wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.80mmol/g; Do not directly contact the oxidized regenerated cellulose sodium carboxylate fabric.

The schematic diagram of the structure of the acidification rotary reactor is shown in Figure 1, the cross section of the acidification rotary reactor is shown in Figure 2, the cross section of the acidification rotary reactor is shown in Figure 3, and the glass porous core 1 is located on the axis of the acidification rotary reactor. core, the direction of rotation is shown in Figure 1.

During the acidification process, the hydrochloric acid is carried from the raised corrugation 2 inside the acidification rotary reactor to the top inside the acidification rotary reactor, and then falls by gravity and is wound on the oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor touch.

Titration of the carboxyl functional group content of the water-soluble hemostatic material in this embodiment is 1.47 mmol/g, indicating that the acidification degree is 37.52%.

In this embodiment, the water-soluble hemostatic material can quickly form a gel within 60 seconds after encountering water or saline solution.

Specific embodiment twenty-two: In this embodiment, the water-soluble hemostatic material is prepared according to the following steps:

Wrap 5.00 g of oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor, then add 61.75 ml of hydrochloric acid with a concentration of 0.20 mol/L, and conduct airtight acidification for 48 hours at a temperature of 25 °C, and then use Water-soluble hemostatic material was obtained by vacuum filtration and washing with absolute ethanol for 5 times, and then freeze-dried at -50°C for 72 hours;

Wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.80mmol/g; Do not directly contact the oxidized regenerated cellulose sodium carboxylate fabric.

The schematic diagram of the structure of the acidification rotary reactor is shown in Figure 1, the cross section of the acidification rotary reactor is shown in Figure 2, the cross section of the acidification rotary reactor is shown in Figure 3, and the glass porous core 1 is located on the axis of the acidification rotary reactor. core, the direction of rotation is shown in Figure 1.

During the acidification process, the hydrochloric acid is carried from the raised corrugation 2 inside the acidification rotary reactor to the top inside the acidification rotary reactor, and then falls by gravity and is wound on the oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor touch.

Titration of the carboxyl functional group content of the water-soluble hemostatic material in this embodiment is 2.49 mmol/g, indicating that the acidification degree is 62.17%.

In this embodiment, the water-soluble hemostatic material can quickly form a gel within 60 seconds after encountering water or saline solution.

Specific embodiment twenty-three: In this embodiment, the water-soluble hemostatic material is prepared according to the following steps:

Wrap 5.00 g of oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor, then add 78.89 ml of hydrochloric acid with a concentration of 0.20 mol/L, and conduct airtight acidification for 48 hours at a temperature of 25° C. Water-soluble hemostatic material was obtained by vacuum filtration and washing with absolute ethanol for 5 times, and then freeze-dried at -50°C for 72 hours;

Wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.80mmol/g; Do not directly contact the oxidized regenerated cellulose sodium carboxylate fabric.

The schematic diagram of the structure of the acidification rotary reactor is shown in Figure 1, the cross section of the acidification rotary reactor is shown in Figure 2, the cross section of the acidification rotary reactor is shown in Figure 3, and the glass porous core 1 is located on the axis of the acidification rotary reactor. core, the direction of rotation is shown in Figure 1.

During the acidification process, the hydrochloric acid is carried from the raised corrugation 2 inside the acidification rotary reactor to the top inside the acidification rotary reactor, and then falls by gravity and is wound on the oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor touch.

Titration of the carboxyl functional group content of the water-soluble hemostatic material in this embodiment is 3.39 mmol/g, indicating that the acidification degree is 83.04%.

In this embodiment, the water-soluble hemostatic material can rapidly form a gel within 120 seconds after encountering water or saline solution.

Specific Embodiment 24: In this embodiment, the water-soluble hemostatic material is prepared according to the following steps:

Wrap 5.00 g of oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor, then add 95.00 ml of hydrochloric acid with a concentration of 0.20 mol/L, and conduct airtight acidification for 48 hours at a temperature of 25 °C, and then use Water-soluble hemostatic material was obtained by vacuum filtration and washing with absolute ethanol for 5 times, and then freeze-dried at -50°C for 72 hours;

Wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.80mmol/g; Do not directly contact the oxidized regenerated cellulose sodium carboxylate fabric.

The schematic diagram of the structure of the acidification rotary reactor is shown in Figure 1, the cross section of the acidification rotary reactor is shown in Figure 2, the cross section of the acidification rotary reactor is shown in Figure 3, and the glass porous core 1 is located on the axis of the acidification rotary reactor. core, the direction of rotation is shown in Figure 1.

During the acidification process, the hydrochloric acid is carried from the raised corrugation 2 inside the acidification rotary reactor to the top inside the acidification rotary reactor, and then falls by gravity and is wound on the oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor touch.

Titration of the carboxyl functional group content of the water-soluble hemostatic material in this embodiment is 3.98 mmol/g, indicating that the acidification degree is 96.33%.

In this embodiment, the water-soluble hemostatic material can rapidly form a gel within 120 seconds after encountering water or saline solution.

Specific embodiment twenty-five: In this embodiment, the water-soluble hemostatic material is prepared according to the following steps:

Wrap 5.00 g of oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor, then add 95.00 ml of hydrochloric acid with a concentration of 0.20 mol/L, and conduct airtight acidification for 48 hours at a temperature of 25 °C, and then use Water-soluble hemostatic material was obtained by vacuum filtration and washing with absolute ethanol for 5 times, and then freeze-dried at -50°C for 72 hours;

Wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.80mmol/g; Do not directly contact the oxidized regenerated cellulose sodium carboxylate fabric.

The schematic diagram of the structure of the acidification rotary reactor is shown in Figure 1, the cross section of the acidification rotary reactor is shown in Figure 2, the cross section of the acidification rotary reactor is shown in Figure 3, and the glass porous core 1 is located on the axis of the acidification rotary reactor. core, the direction of rotation is shown in Figure 1.

During the acidification process, the hydrochloric acid is carried from the raised corrugation 2 inside the acidification rotary reactor to the top inside the acidification rotary reactor, and then falls by gravity and is wound on the oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor touch.

Titration of the carboxyl functional group content of the water-soluble hemostatic material in this embodiment is 3.98 mmol/g, indicating that the acidification degree is 96.33%.

In this embodiment, the water-soluble hemostatic material can rapidly form a gel within 120 seconds after encountering water or saline solution.

Specific embodiment twenty-six: In this embodiment, the water-soluble hemostatic material is prepared according to the following steps:

Wrap 5.00 g of oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor, then add 190.00 ml of hydrochloric acid with a concentration of 0.20 mol/L, and conduct airtight acidification for 48 hours at a temperature of 25 °C, and then use Water-soluble hemostatic material was obtained by vacuum filtration and washing with absolute ethanol for 5 times, and then freeze-dried at -50°C for 72 hours;

Wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.80mmol/g; Do not directly contact the oxidized regenerated cellulose sodium carboxylate fabric.

The schematic diagram of the structure of the acidification rotary reactor is shown in Figure 1, the cross section of the acidification rotary reactor is shown in Figure 2, the cross section of the acidification rotary reactor is shown in Figure 3, and the glass porous core 1 is located on the axis of the acidification rotary reactor. core, the direction of rotation is shown in Figure 1.

During the acidification process, the hydrochloric acid is carried from the raised corrugation 2 inside the acidification rotary reactor to the top inside the acidification rotary reactor, and then falls by gravity and is wound on the oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor touch.

Titration of the carboxyl functional group content of the water-soluble hemostatic material in this embodiment is 3.99 mmol/g, indicating that the acidification degree is 96.58%.

In this embodiment, the water-soluble hemostatic material can rapidly form a gel within 120 seconds after encountering water or saline solution.

Specific embodiment twenty-seven: In this embodiment, the water-soluble hemostatic material is prepared according to the following steps:

Wrap 5.00 g of oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor, then add 190.00 ml of hydrochloric acid with a concentration of 0.20 mol/L, and conduct airtight acidification for 48 hours at a temperature of 25 °C, and then use Water-soluble hemostatic material was obtained by vacuum filtration and washing with absolute ethanol for 5 times, and then freeze-dried at -50°C for 72 hours;

Wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.80mmol/g; Do not directly contact the oxidized regenerated cellulose sodium carboxylate fabric.

The schematic diagram of the structure of the acidification rotary reactor is shown in Figure 1, the cross section of the acidification rotary reactor is shown in Figure 2, the cross section of the acidification rotary reactor is shown in Figure 3, and the glass porous core 1 is located on the axis of the acidification rotary reactor. core, the direction of rotation is shown in Figure 1.

During the acidification process, the hydrochloric acid is carried from the raised corrugation 2 inside the acidification rotary reactor to the top inside the acidification rotary reactor, and then falls by gravity and is wound on the oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor touch.

Titration of the carboxyl functional group content of the water-soluble hemostatic material in this embodiment is 3.99 mmol/g, indicating that the acidification degree is 96.58%.

In this embodiment, the water-soluble hemostatic material can rapidly form a gel within 120 seconds after encountering water or saline solution.

Specific embodiment twenty-eight: In this embodiment, the water-soluble hemostatic material is prepared according to the following steps:

Wrap 5.00 g of oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor, then add 95.00 ml of hydrochloric acid with a concentration of 2.00 mol/L, and carry out airtight acidification for 48 hours at a temperature of 25 °C, and then use Water-soluble hemostatic material was obtained by vacuum filtration and washing with absolute ethanol for 5 times, and then freeze-dried at -50°C for 72 hours;

Wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.80mmol/g; Do not directly contact the oxidized regenerated cellulose sodium carboxylate fabric.

The schematic diagram of the structure of the acidification rotary reactor is shown in Figure 1, the cross section of the acidification rotary reactor is shown in Figure 2, the cross section of the acidification rotary reactor is shown in Figure 3, and the glass porous core 1 is located on the axis of the acidification rotary reactor. core, the direction of rotation is shown in Figure 1.

During the acidification process, the hydrochloric acid is carried from the raised corrugation 2 inside the acidification rotary reactor to the top inside the acidification rotary reactor, and then falls by gravity and is wound on the oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core 1 of the acidification rotary reactor touch.

Titration of the carboxyl functional group content of the water-soluble hemostatic material in this embodiment is 3.96 mmol/g, indicating that the acidification degree is 95.76%.

In this embodiment, the water-soluble hemostatic material can rapidly form a gel within 120 seconds after encountering water or saline solution.

Hemostatic performance comparison experiment:

Select 20 New Zealand white rabbits with a body weight of 3.0Kg, and randomly divide them into 4 groups of 5 each; the corresponding test materials are respectively: common sterile gauze (blank control group), oxidized regenerated cellulose (positive control group), specific implementation methods 20 A prepared water-soluble hemostatic material (with a carboxyl functional group content of 1.47 mmol/g) and Embodiment 14 A prepared water-soluble hemostatic material (with a carboxyl functional group content of 0.11 mmol/g). New Zealand white rabbits were fixed on the dissection table, and 1ml of 2.5% pentobarbital sodium solution was slowly injected after disinfection of the ear veins. After the rabbits were anesthetized, the abdominal cavity was opened layer by layer, and the tissue fluid and blood, exposing the liver. A wound of 1.0 cm×1.0 cm×0.3 cm was made on the liver lobe with a scalpel. During the process of wound formation, oozing blood is first absorbed with sterilized medical gauze, and then the above-mentioned hemostatic material of 1.5cm×1.5cm is applied to the wound surface, and the sterilized medical gauze is used as an aid in compressing, and the hemostatic effect is observed and timed with a stopwatch , observe the hemostatic effect, and record the hemostatic time after complete hemostasis; repeat the test 5 times for each material, and record the complete hemostatic time respectively, the results are shown in Table 1.

Table 1

Test material Average hemostasis time (min) Ordinary sterile gauze ＞15 oxidized regenerated cellulose 3.6 Specific implementation methods 21 Prepared water-soluble hemostatic material 2.7 Specific Embodiment 14 Water-soluble hemostatic material prepared 2.2

The experimental results show that the water-soluble hemostatic material has a faster hemostasis speed.

The oxidized regenerated cellulose hemostatic material was placed on the wound surface, and after 60 seconds, it was observed that the oxidized regenerated cellulose hemostatic material was still located on the wound surface, and the bleeding did not improve (as shown in Figure 4). The water-soluble hemostatic material prepared in this embodiment was placed on the wound surface, and after 60 seconds, it was observed that the water-soluble hemostatic material prepared in this embodiment had merged with the liver of New Zealand white rabbits and became a transparent gel (as shown in Figure 5 ), and seal the wound to achieve hemostasis.

Biodegradation performance comparison experiment:

18 New Zealand white rabbits with a body weight of 3.0Kg were selected, randomly divided into 3 groups of 6, and the corresponding test materials were respectively: oxidized regenerated cellulose (control group), water-soluble hemostatic material (carboxyl functional group) prepared in specific embodiment 21 content of 1.47mmol/g) and the water-soluble hemostatic material prepared in Embodiment 14 (carboxyl functional group content of 0.11mmol/g). New Zealand white rabbits were fixed on the dissecting table, and 1ml of 2.5% pentobarbital sodium solution was slowly injected to anesthetize the rabbits after disinfection of the ear veins. Incisions were made on both sides of the spine, and test materials were implanted in the muscles with a size of 2.0cm×0.5cm, and then sutured. Take out the muscle tissue and implanted material at the implanted site 1 day, 3 days, 7 days, 10 days, 14 days, and 21 days after the operation, and make pathological section observations to summarize the time when the implanted material is completely absorbed by the organism , the experimental results are shown in Table 2.

Table 2

Test material Complete absorption time (days) oxidized regenerated cellulose 14～21 Specific implementation methods 21 Prepared water-soluble hemostatic material 7～10 Specific Embodiment 14 Water-soluble hemostatic material prepared 5～7

The experimental results show that the water-soluble hemostatic material has good bioabsorbability.

Claims (10)

1. Water-soluble hemostatic material, characterized in that the water-soluble hemostatic material is made of oxidized regenerated cellulose sodium carboxylate fabric and hydrochloric acid, the carboxyl functional group content in the water-soluble hemostatic material is 0.10～5.21mmol/g, and the oxidized regenerated cellulose carboxylic acid The degree of acidification of sodium carboxylate in the sodium fabric is 3% to 98%; wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.30 to 4.77mmol/g, and the carboxylate in the oxidized regenerated cellulose sodium carboxylate fabric The molar ratio of sodium carboxylate to hydrogen ions in hydrochloric acid is (100:1)～(1:20), wherein the concentration of carboxylic acid functional groups in the water-soluble hemostatic material presents a gradient distribution, and the content of sodium carboxylate functional groups is shown in the table and The content of carboxylic acid functional groups gradually decreased from the surface to the inside. 2. The water-soluble hemostatic material according to claim 1, characterized in that the carboxyl functional group content in the water-soluble hemostatic material is 0.17 to 2.25 mmol/g, and the acidification degree of sodium carboxylate in the oxidized regenerated cellulose sodium carboxylate fabric is 5. %~45%. 3. The water-soluble hemostatic material according to claim 1, characterized in that the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.40-4.14 mmol/g. 4. The water-soluble hemostatic material according to claim 1, characterized in that the molar ratio of sodium carboxylate in the oxidized regenerated cellulose sodium carboxylate fabric to hydrogen ions in hydrochloric acid is (4:1) to (1:10). 5. The preparation method of water-soluble hemostatic material as claimed in claim 1, characterized in that the water-soluble hemostatic material is prepared according to the following steps: Wrap the oxidized regenerated cellulose sodium carboxylate fabric on the glass porous core of the acidification rotary reactor, then add hydrochloric acid for closed acidification for 0.5-72 hours, the acidification temperature is 10-30°C, and then rinse with absolute ethanol for 3-3~ 5 times, and then freeze-dried in an environment of -10 to -80°C for 24 to 120 hours to obtain a water-soluble hemostatic material; Wherein, the sodium carboxylate functional group content in the oxidized regenerated cellulose sodium carboxylate fabric is 3.30～4.77mmol/g, and the molar ratio of sodium carboxylate and hydrogen ion in the hydrochloric acid in the oxidized regenerated cellulose sodium carboxylate fabric is (100:1) ~(1:20); the rotating speed of the acidification rotary reactor is 100～400r/min, and the hydrochloric acid added to the acidification rotary reactor does not directly contact with the oxidized regenerated cellulose sodium carboxylate fabric when the acidification rotary reactor does not start . 6. The preparation method of the water-soluble hemostatic material according to claim 5, characterized in that the concentration of hydrochloric acid is 0.01-2.00 mol/L. 7. The preparation method of the water-soluble hemostatic material according to claim 6, characterized in that oxidized regenerated cellulose carboxyl The content of sodium carboxylate functional group in sodium acid fabric is 3.40～4.14mmol/g. 8. The preparation method of the water-soluble hemostatic material according to claim 7, characterized in that oxidized regenerated cellulose carboxyl The molar ratio of sodium carboxylate to hydrogen ions in hydrochloric acid in sodium acid fabric is (4:1)～(1:10). 9. The preparation method of the water-soluble hemostatic material according to claim 8, characterized in that the acidification time is 8-48 hours, and the acidification temperature is 20-25°C. 10. The method for preparing the water-soluble hemostatic material according to claim 8, characterized in that the freeze-drying temperature is -20--65°C, and the freeze-drying time is 48-72 hours.

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