CN102187839B - Artificial crossing cultivation method of seawater takifugu species - Google Patents
Artificial crossing cultivation method of seawater takifugu species Download PDFInfo
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- CN102187839B CN102187839B CN2011101465924A CN201110146592A CN102187839B CN 102187839 B CN102187839 B CN 102187839B CN 2011101465924 A CN2011101465924 A CN 2011101465924A CN 201110146592 A CN201110146592 A CN 201110146592A CN 102187839 B CN102187839 B CN 102187839B
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- 239000013535 sea water Substances 0.000 title claims description 5
- 238000012364 cultivation method Methods 0.000 title 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 17
- 229940088597 hormone Drugs 0.000 claims description 15
- 239000005556 hormone Substances 0.000 claims description 15
- 230000012447 hatching Effects 0.000 claims description 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 9
- 239000004568 cement Substances 0.000 claims description 9
- 230000026109 gonad development Effects 0.000 claims description 9
- 238000005286 illumination Methods 0.000 claims description 9
- 229910052760 oxygen Inorganic materials 0.000 claims description 9
- 239000001301 oxygen Substances 0.000 claims description 9
- 230000032696 parturition Effects 0.000 claims description 9
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- 235000007516 Chrysanthemum Nutrition 0.000 claims description 7
- 241000980706 Takifugu obscurus Species 0.000 claims description 7
- 210000004907 gland Anatomy 0.000 claims description 7
- 238000009396 hybridization Methods 0.000 claims description 7
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- 244000068988 Glycine max Species 0.000 claims description 6
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
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- 241000239250 Copepoda Species 0.000 claims description 3
- 102000002322 Egg Proteins Human genes 0.000 claims description 3
- 108010000912 Egg Proteins Proteins 0.000 claims description 3
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- 239000009636 Huang Qi Substances 0.000 claims description 3
- 240000002817 Leonurus sibiricus Species 0.000 claims description 3
- 235000002434 Leonurus sibiricus Nutrition 0.000 claims description 3
- 244000241872 Lycium chinense Species 0.000 claims description 3
- 235000015468 Lycium chinense Nutrition 0.000 claims description 3
- 241001465754 Metazoa Species 0.000 claims description 3
- 241000595940 Notostraca Species 0.000 claims description 3
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- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims description 3
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 3
- 102000005320 Posterior Pituitary Hormones Human genes 0.000 claims description 3
- 108010070873 Posterior Pituitary Hormones Proteins 0.000 claims description 3
- 210000001015 abdomen Anatomy 0.000 claims description 3
- 238000005273 aeration Methods 0.000 claims description 3
- 239000012297 crystallization seed Substances 0.000 claims description 3
- 235000013399 edible fruits Nutrition 0.000 claims description 3
- 235000013345 egg yolk Nutrition 0.000 claims description 3
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- 235000018905 epimedium Nutrition 0.000 claims description 3
- 239000013505 freshwater Substances 0.000 claims description 3
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- 230000009027 insemination Effects 0.000 claims description 3
- 239000008267 milk Substances 0.000 claims description 3
- 235000013336 milk Nutrition 0.000 claims description 3
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- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 230000000384 rearing effect Effects 0.000 claims description 3
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- 238000012216 screening Methods 0.000 claims description 3
- 239000002689 soil Substances 0.000 claims description 3
- 230000001550 time effect Effects 0.000 claims description 3
- 230000007704 transition Effects 0.000 claims description 3
- 244000189548 Chrysanthemum x morifolium Species 0.000 claims 1
- 241001016310 Epimedium grandiflorum Species 0.000 claims 1
- 239000000872 buffer Substances 0.000 description 8
- 241000723353 Chrysanthemum Species 0.000 description 6
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses an artificial crossing cultivation method of seawater takifugu species. The method comprises screening, cultivating and artificial propagation of parent takifugu, incubating, fingerling cultivating and other processes, and is characterized in that on the basis of fully grasping the breeding ecological environment and gonad growth rules of takifugu obscurus and takifugu flavidus, the takifugu flavidus is used as a female parent and the relatively large takifugu obscurus is used as a male parent to simulate a natural ecological environment; mature gonad and ovulation of the parent takifugu are realized by hormone control; an artificial insemination method is employed to realize hybridization among the takifugu species. Synchronously, in the hybridization process, the ecology management is enhanced; and therefore, the oxytocic rate of the hybridization reaches 95 percent, the fertilization rate, the hatchability rate and the opening rate all reach above 80 percent, and the aberration rate of the fingerlings is below 2.0 thousandth.
Description
Technical field
The invention belongs to the fishery resources technical field.Relate in particular to Fugu and belong to breeding technique, a kind of more precisely use the yellow Fugu of female chrysanthemum and male fugu obscurus carry out Fugu belong to kind between the method for artificial hybridization cultivation.
Background technology
Fugu belongs to (Fugu), is commonly called as filefish, creek fish, crust fish.Because the Fugu deterioration of the ecological environment, cruel in addition fishing is excessively captured, and Fugu quantity sharply descends in recent years, so people have carried out the artificial breeding technology research of fugu obscurus and the yellow Fugu of chrysanthemum, has formed one and has overlapped perfect people's propagating technology.Owing to the breakthrough of fugu obscurus and the yellow Fugu artificial propagation techniques of chrysanthemum, the breed of river Puffer fish is flourish trend in recent years, when obtaining distinct economic, owing to its long-term artificial propagation and breed, has occurred the phenomenon of deterioration of variety.Parent's quality can't be guaranteed in the artificial propagation process, and disease takes place frequently in the breeding process, and individual growth is uneven; Specification diminishes gradually, and these phenomenons have had a strong impact on the breed development of Fugu, in order to protect this precious stock of fish of Fugu; Realize long-term artificial propagation and the breed of Fugu; Must set about from breed improvement and seed selection, utilize existing technology and means, seed selection and cultivate fast, individual big, the Fugu kind that disease is few of growth.
Summary of the invention
The objective of the invention is to solve the technical barrier that the Fugu of always failing to capture for a long time belongs to interspecific cross; For Fugu belongs to the interspecific cross breeding a kind of feasible method is provided; The artificial propagation and the breed breeding of river Puffer lay the foundation; Be expected to cultivate thus good Puffer class new varieties, improve the economic benefit that the river Puffer is cultured, promote the development that China's Fugu culture fishery is healthy, stable, continue.
The present invention adopts following technical scheme for realizing above-mentioned purpose:
The method that artificial hybridization was cultivated between a kind of seawater Fugu genus was planted may further comprise the steps:
(1) select the parent: pick out individual big and healthy and strong, disease-free, do not have wounded or disabled, body surface does not have parasite, be in a good state of nutrition, the yellow Fugu of the normal chrysanthemum of gonad development is as female parent, the bigger fugu obscurus of individuality is a male parent;
(2) parent culture: male and female parent population individuality is put into indoor cement pit cultivate, cultivate density 1-10 tail/m
3, cement pit area 8-15m
2, the depth of water is greater than 100cm, and dissolved oxygen maintains more than the 5mg/L, illumination 600-800Lux, miniflow water, day is changed water 1-2 time; Be main with the animal bait of throwing something and feeding during the parent culture; Progressively to the transition of high protein mixed bait; The fish of in manufactured feed, adding 30-90ppm with hormone LH-RH-A, 1 ‰ by longspur epimedium, the bark of eucommia, when ginseng or radix scrophulariae, Radix Glycyrrhizae, the Radix Astragali, the fruit of Chinese wolfberry, siberian motherwort, Chinese herbal medicine that black soybean is formed, 1 ‰ VE and 1 ‰ edible salt; Every morning 8:00-9:00, afternoon 4:00-5:00 and evening 9:00-10:00 respectively feed once; Each feeding volume is the 3-7% of parent population gross weight, and first soil pick-up and additional fresh water aeration are kept 18 ± 0.5 ℃ of water temperatures before each bait throwing in;
(3) artificial insemination: hastening parturition preceding 30 and 60 days, and respectively the direct injection of exogenous hormone of parent population body LRHA was being carried out twice and accelerate the ripening in advance, the parent population ID is 0.1 μ g/kg for the first time, for the second time parent population ID 0.2 μ g/kg; Observe parent population gonad development situation, find that parent population belly outward appearance expands, sponginess and flexible, gonopore is rubescent and be when parting out state a little, carries out artificial induced spawning; When artificial induced spawning is annotated,, utilize hormone to promote the parent population sexual gland to transit to the V phase from the IV phase according to the gonad development state, and make it ovulation, row is smart; The IV phase is injected hormone LRH-A, and consumption is 0.4-0.6 μ g/kg, and the injection 0.5 μ g/kg pituitrin of hastening parturition for the last time amounts to and hastens parturition hormone time effect 36h 3-5 time; After treating parent population sexual gland full maturity, carry out wet method or dry method of artificial fertilization.
(4) hatch: test-tube fish-egg is placed in the hatching pail hatches brooding time 4-6 days; Water temperature is 22 ± 1 ℃ in the incubator,, dissolved oxygen maintains more than the 5mg/L, increases intensity of illumination gradually, hatching early stage 1000Lux, the later stage is increased to 1500 Lux, salinity is less than 1.0 ‰, hatching pail diameter 1.0-1.3m, high 1.2-1.5m.
(5) seed rearing: in incubator, cultivated 1-3 days earlier behind the fry hatching, then seed is moved to 40m
2Cultivate in the cement pit; Hatch in 3-6 days, with 100 purpose bolting silks screening egg yolk, the seed of throwing something and feeding, throw something and feed in 7-10 days the fairy shrimp young or little copepoda; Water temperature is 22 ± 1 ℃ during cultivation, and dissolved oxygen maintains more than the 5mg/L, and intensity of illumination is greater than 1500Lux, and salinity is less than 1.0 ‰; When treating seed length to 0.8-1.5cm, band water moves to seed in the outdoor earth pond pond raises and train, and raises and train preceding pond full pool spilling head soya-bean milk or fertilising, sends out the pool.
The present invention is the breakthrough first of artificial hybridization between the Fugu genus is planted, for the artificial propagation and the breed breeding of river Puffer are laid a good foundation.The crossbreeding that belongs between planting for Fugu provides a feasible approach, is expected to cultivate thus good Puffer class and cultures new varieties.The female parent that the present invention selects for use is the yellow Fugu of chrysanthemum; Male parent is individual bigger fugu obscurus; Adopted reinforced cultivating and preparatory ripening technique during the parent culture, promoted the growth of parent population sexual gland, the spawning rate of hybridization has reached 95% simultaneously; Fertilization rate, incubation rate and aperture opening ratio have all reached more than 80%, and the seed abnormal rate is below 2.0 ‰.The invention solves owing to long-term artificial propagation and breed, the phenomenon of river Puffer deterioration of variety supports for river Puffer healthy aquaculture provides seed, promotes the development that China's culture fishery is healthy, stablize, continue.
Embodiment
The method that artificial hybridization was cultivated between a kind of seawater Fugu genus was planted may further comprise the steps:
(1) select the parent: pick out individual big and healthy and strong, disease-free, do not have wounded or disabled, body surface does not have parasite, be in a good state of nutrition, the yellow Fugu of the normal chrysanthemum of gonad development is as female parent, the bigger fugu obscurus of individuality is a male parent;
(2) parent culture: male and female parent population individuality is put into indoor cement pit cultivate, cultivate density 1-10 tail/m
3, cement pit area 8-15m
2, the depth of water is greater than 100cm, and dissolved oxygen maintains more than the 5mg/L, illumination 600-800Lux, miniflow water, day is changed water 1-2 time; Be main with the animal bait of throwing something and feeding during the parent culture; Progressively to the transition of high protein mixed bait; The fish of in manufactured feed, adding 30-90ppm with hormone LH-RH-A, 1 ‰ by longspur epimedium, the bark of eucommia, when ginseng or radix scrophulariae, Radix Glycyrrhizae, the Radix Astragali, the fruit of Chinese wolfberry, siberian motherwort, Chinese herbal medicine that black soybean is formed, 1 ‰ VE and 1 ‰ edible salt; Every morning 8:00-9:00, afternoon 4:00-5:00 and evening 9:00-10:00 respectively feed once; Each feeding volume is the 3-7% of parent population gross weight, and first soil pick-up and additional fresh water aeration are kept 18 ± 0.5 ℃ of water temperatures before each bait throwing in;
(3) artificial insemination: hastening parturition preceding 30 and 60 days, and respectively the direct injection of exogenous hormone of parent population body LRHA was being carried out twice and accelerate the ripening in advance, the parent population ID is 0.1 μ g/kg for the first time, for the second time parent population ID 0.2 μ g/kg; Observe parent population gonad development situation, find that parent population belly outward appearance expands, sponginess and flexible, gonopore is rubescent and be when parting out state a little, carries out artificial induced spawning; When artificial induced spawning is annotated,, utilize hormone to promote the parent population sexual gland to transit to the V phase from the IV phase according to the gonad development state, and make it ovulation, row is smart; The IV phase is injected hormone LRHA, and consumption is 0.4-0.6 μ g/kg, and the injection 0.5 μ g/kg pituitrin of hastening parturition for the last time amounts to and hastens parturition hormone time effect 36h 3-5 time; After treating parent population sexual gland full maturity, carry out wet method or dry method of artificial fertilization.
(4) hatch: test-tube fish-egg is placed in the hatching pail hatches brooding time 4-6 days; Water temperature is 22 ± 1 ℃ in the incubator,, dissolved oxygen maintains more than the 5mg/L, increases intensity of illumination gradually, hatching early stage 1000Lux, the later stage is increased to 1500 Lux, salinity is less than 1.0 ‰, hatching pail diameter 1.0-1.3m, high 1.2-1.5m.
(5) seed rearing: in incubator, cultivated 1-3 days earlier behind the fry hatching, then seed is moved to 40m
2Cultivate in the cement pit; Hatch in 3-6 days, with 100 purpose bolting silks screening egg yolk, the seed of throwing something and feeding, throw something and feed in 7-10 days the fairy shrimp young or little copepoda; Water temperature is 22 ± 1 ℃ during cultivation, and dissolved oxygen maintains more than the 5mg/L, and intensity of illumination is greater than 1500Lux, and salinity is less than 1.0 ‰; When treating seed length to 0.8-1.5cm, band water moves to seed in the outdoor earth pond pond raises and train, and raises and train preceding pond full pool spilling head soya-bean milk or fertilising, sends out the pool.
Claims (1)
1. the method that artificial hybridization was cultivated between a seawater Fugu genus was planted may further comprise the steps:
(1) select the parent: pick out individual big and healthy and strong, disease-free, do not have wounded or disabled, body surface does not have parasite, be in a good state of nutrition, the yellow Fugu of the normal chrysanthemum of gonad development is as female parent, the bigger fugu obscurus of individuality is a male parent;
(2) parent culture: male and female parent population individuality is put into indoor cement pit cultivate, cultivate density 1-10 tail/m
3, cement pit area 8-15m
2, the depth of water is greater than 100cm, and dissolved oxygen maintains more than the 5mg/L, illumination 600-800Lux, miniflow water, day is changed water 1-2 time; Be main with the animal bait of throwing something and feeding during the parent culture; Progressively to the transition of high protein mixed bait; The fish of in manufactured feed, adding 30-90ppm with hormone LH-RH-A, 1 ‰ by barrenwort, the bark of eucommia, when ginseng or radix scrophulariae, Radix Glycyrrhizae, the Radix Astragali, the fruit of Chinese wolfberry, siberian motherwort, Chinese herbal medicine that black soybean is formed, 1 ‰ VE and 1 ‰ edible salt; Every morning 8:00-9:00, afternoon 4:00-5:00 and evening 9:00-10:00 respectively feed once; Each feeding volume is the 3-7% of parent population gross weight, and first soil pick-up and additional fresh water aeration are kept 18 ± 0.5 ℃ of water temperatures before each bait throwing in;
(3) artificial insemination: hastening parturition preceding 30 and 60 days, and respectively the direct injection of exogenous hormone of parent population body LRH-A was being carried out twice and accelerate the ripening in advance, the parent population ID is 0.1 μ g/kg for the first time, for the second time parent population ID 0.2 μ g/kg; Observe parent population gonad development situation, find that parent population belly outward appearance expands, sponginess and flexible, gonopore is rubescent and be when parting out state a little, carries out artificial induced spawning; When artificial induced spawning is annotated,, utilize hormone to promote the parent population sexual gland to transit to the V phase from the IV phase according to the gonad development state, and make it ovulation, row is smart; The IV phase is injected hormone LRHA, and consumption is 0.4-0.6 μ g/kg, and the injection 0.5 μ g/kg pituitrin of hastening parturition for the last time amounts to and hastens parturition hormone time effect 36h 3-5 time; After treating parent population sexual gland full maturity, carry out wet method or dry method of artificial fertilization;
(4) hatch: test-tube fish-egg is placed in the hatching pail hatches brooding time 4-6 days; Water temperature is 22 ± 1 ℃ in the incubator,, dissolved oxygen maintains more than the 5mg/L, increases intensity of illumination gradually, hatching early stage 1000Lux, the later stage is increased to 1500 Lux, salinity is less than 1.0 ‰, hatching pail diameter 1.0-1.3m, high 1.2-1.5m;
(5) seed rearing: in incubator, cultivated 1-3 days earlier behind the fry hatching, then seed is moved to 40m
2Cultivate in the cement pit; Hatch in 3-6 days, with 100 purpose bolting silks screening egg yolk, the seed of throwing something and feeding, throw something and feed in 7-10 days the fairy shrimp young or little copepoda; Water temperature is 22 ± 1 ℃ during cultivation, and dissolved oxygen maintains more than the 5mg/L, and intensity of illumination is greater than 1500Lux, and salinity is less than 1.0 ‰; When treating seed length to 0.8-1.5cm, band water moves to seed in the outdoor earth pond pond raises and train, and raises and train preceding pond full pool spilling head soya-bean milk or fertilising, sends out the pool.
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| CN105010193B (en) * | 2015-07-27 | 2017-10-24 | 上海市水产研究所 | One kind hybridizes Fugu seed pond breeding method then |
| CN105123573B (en) * | 2015-07-27 | 2017-10-24 | 上海市水产研究所 | Fugu obscurus raun and takifugu flavidus milter cross breeding method |
| KR101632842B1 (en) * | 2015-11-18 | 2016-06-23 | 충청남도 | Hybridized species of olive takifugu obscurus and takifugu rubripes, and process for preparing the same |
| CN107624682A (en) * | 2017-08-22 | 2018-01-26 | 大连富泰水产养殖有限公司 | A kind of Fugu rubripes cultural method |
| CN110050731A (en) * | 2019-05-29 | 2019-07-26 | 江苏中洋集团股份有限公司 | The high globe fish cultural method of toxin |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101032228A (en) * | 2006-03-09 | 2007-09-12 | 上海能正渔业科技开发有限公司 | Establishing and culture selecting method of breeding a pure system of fugu obscurus |
| CN101248770A (en) * | 2008-03-28 | 2008-08-27 | 河北省水产研究所 | Method for shortening cultivation period of Fugu flavidus |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101032228A (en) * | 2006-03-09 | 2007-09-12 | 上海能正渔业科技开发有限公司 | Establishing and culture selecting method of breeding a pure system of fugu obscurus |
| CN101248770A (en) * | 2008-03-28 | 2008-08-27 | 河北省水产研究所 | Method for shortening cultivation period of Fugu flavidus |
Non-Patent Citations (1)
| Title |
|---|
| 施永海等.温度对暗纹东方魨和菊黄东方魨幼鱼瞬时耗氧速率的影响.《上海海洋大学学报》.2011,第20卷(第1期),第50-55页. * |
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